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Lactobacillus plantarum BUFX and application thereof in metabolic syndrome

A technology of Lactobacillus plantarum and metabolic syndrome, applied in the field of microbiology and its application, can solve problems such as differences in the influence of intestinal flora, complex pathogenesis of diabetes, etc., achieve excellent hypoglycemic ability, improve glucose tolerance and insulin resistance, reduce Effects of Body Weight and Lipid Levels

Active Publication Date: 2021-08-27
BEIJING QUANTIHEALTH TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, my country has published 35 kinds or subspecies of edible probiotics, but due to the complex pathogenesis of diabetes and the strain-specific functions of probiotics, their effects on intestinal flora and host metabolism are also different

Method used

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  • Lactobacillus plantarum BUFX and application thereof in metabolic syndrome
  • Lactobacillus plantarum BUFX and application thereof in metabolic syndrome
  • Lactobacillus plantarum BUFX and application thereof in metabolic syndrome

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1: Sample collection and strain isolation

[0043] The feces of healthy adults living in the urban area of ​​Beijing for a long time were collected as screening samples. The collected subjects had never taken antibiotics, probiotics, or gastrointestinal diseases before collection. Dilute the collected feces samples and spread them on YCFA medium, culture them anaerobically at 37°C for 24-48 hours, and separate and obtain different single colonies on the plate. Pick different single colonies with a sterilized inoculation loop, streak and purify them on a new YCFA solid medium plate, culture anaerobically at 37°C for 48 hours, and obtain 29 purified colonies, which are marked as L1-L29 respectively.

[0044] It should be noted that the dilution of the stool sample is a conventional method in the art, and the present invention is not limited. In one embodiment of the present invention, the stool sample is diluted with clean water.

Embodiment 2

[0045] Embodiment 2: preparation of living bacteria liquid, metabolites and inactivated bacteria

[0046] 2.1 Culture of bacteria

[0047] Take -80°C frozen bacteria liquid and spread it on the YCFA solid plate, and after inverting culture at 37°C for 24-48 hours, take a single colony and inoculate it in the liquid YCFA medium, and cultivate it at 37°C for 18-24 hours to obtain the first-generation bacterial liquid; Take 10% of the first-generation bacterial solution and inoculate it into fresh YCFA liquid medium, incubate at 37°C for 18-24 hours to obtain the second-generation bacterial solution; take 10% of the second-generation bacterial solution and inoculate it into fresh YCFA liquid medium, and incubate at 37°C for 18-24 hours to obtain working bacteria.

[0048] Obtaining live bacteria

[0049] In an embodiment of the present invention, the working bacterial solution obtained in step 1 was placed at 13,000 rpm and centrifuged at 4°C for 15 minutes, the supernatant was...

Embodiment 3

[0055] Embodiment 3: have the bacterial strain screening and the identification that promotes cell glucose cell effect

[0056] 3.1 Strain identification

[0057] Streak culture the isolated single colony, then pick a single colony and spread it on the mass spectrometer plate, add the lysate and matrix to dry, and then use the MALDI-TOF MS 1000 mass spectrometer (Autobio) to identify each strain. information.

[0058] Cellular glucose consumption assay

[0059] The human liver cancer HepG2 cells used in the present invention were purchased from the National Biomedical Laboratory Cell Resource Bank. The cell culture adopts a culture method known in the art, which is not limited in the present invention.

[0060] Human liver cancer HepG2 cells were cultured at 37°C and 5% CO 2 In high glucose DMEM medium containing 10% fetal bovine serum (FBS) under the condition, double antibodies (100 µg / ml penicillin and 100 µg / ml streptomycin) were added to the medium at a ratio of 1:100...

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Abstract

The invention provides a lactobacillus plantarum BUFX (Lactobacillus plantarum) and application thereof in metabolic syndrome, which relate to the technical field of microorganisms and application of the microorganisms. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.22172. The lactobacillus plantarum is separated and screened from feces of adults, can promote glucose consumption of HepG2 hepatoma carcinoma cells, inhibits alpha-amylase activity, can remarkably reduce the weight and blood fat content of high-fat diet mice, improves glucose tolerance, and has important application significance for preventing or treating metabolic diseases such as diabetes, obesity and the like.

Description

technical field [0001] The present invention relates to the technical field of microbes and their applications, in particular to a plant Lactobacillus ( Lactobacillus plantarum ) BUFX and its application in metabolic syndrome. Background technique [0002] With the improvement of people's living standards and changes in diet structure, eating and drinking are not taboo, eating more and moving less, diabetes is also known as the "disease of the rich". This modern lifestyle tends to cause excess glucose in the body, leading to elevated blood sugar, and long-term hyperglycemia or elevated blood sugar usually leads to diabetes. Over time, diabetes may damage the cardiovascular, kidney, and nervous systems. Diabetes is currently one of the leading causes of blindness, kidney failure, heart attack, stroke, and lower limb amputation, seriously endangering human health. According to the statistics of the World Health Organization, the number of diabetic patients has increased from...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/747A61P3/00A61P3/10A61P3/06A61P1/16A61P19/06A61P7/00A61P9/12A61P3/04A61P5/50A23L33/135C12R1/25
CPCC12N1/20A61K35/747A61P3/00A61P3/10A61P3/06A61P1/16A61P19/06A61P7/00A61P9/12A61P3/04A61P5/50A23L33/135A23V2002/00A23V2400/169A23V2200/328A23V2200/332A23V2200/30A23V2200/3262A23V2250/206
Inventor 徐文艺李转羽赵柏闻韩璐
Owner BEIJING QUANTIHEALTH TECH CO LTD
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