251 results about "Antibiotics" patented technology

The invention discloses an infection risk assessment method and system for burn nursing, and the system comprises a long-term trend analysis module and a short-term anomaly detection module, the long-term trend analysis module employs an improved Transform-XL model, and captures the chronic cumulative effect of inflammation indexes within 72 hours through a relative position coding and memory multiplexing mechanism; the short-term anomaly module is based on a bidirectional LSTM reconstruction network, combines a dynamic threshold algorithm to identify sudden physiological anomaly in a window of 6 hours in real time, generates a final infection risk index through a confidence-driven adaptive fusion algorithm and dynamic weighted dual-channel output, triggers clinical response in combination with a hierarchical early warning mechanism, and introduces closed-loop feedback optimization, so as to achieve the early warning of the physiological anomaly. The actual intervention effect feedback model is iterated, the infection recognition time is shortened to 3.2 hours, the ROC-AUC reaches 0.94, and the reasonable utilization rate of antibiotics is increased to 89%.

The invention discloses a method for traceless gene editing of gram negative bacteria, a carrier and application of the carrier, and belongs to the technical field of microbial genetic engineering. The invention provides a vector for performing traceless gene editing on bordetella pertussis. The vector simultaneously comprises an antibiotic positive screening gene expression box and a sacB and galk negative screening gene expression box. According to the method, the sacB gene and the galk genome are synthesized into the double-reverse-screening system for the first time, and compared with a single-reverse-screening system, the reverse-screening efficiency is remarkably improved, and the dosage of a reverse-screening reagent is remarkably reduced. The invention provides a novel gene editing method for non-mode bacteria such as pertussis bordetella and the like, and has a good application prospect.

The invention relates to a microbial agent prepared based on a new strain of bacillus velezensis, which has the function of broad-spectrum antagonism of common pathogenic bacteria of aquatic products and can effectively prevent and / or treat bacterial diseases and / or fungal diseases of aquatic organisms including crustaceans and fishes. Meanwhile, the content of nitrite and ammonia nitrogen in aquaculture water can be effectively reduced, and the usage amount and residual quantity of antibiotics and chemical drugs in aquaculture are reduced.

The invention discloses a high-valued graded utilization method of marine shells, and belongs to the technical field of comprehensive utilization of biological resources and preparation of functional materials. The method comprises the following steps: cleaning, air-drying and superfine grinding marine shell wastes such as abalone shells, and extracting water-soluble organic components in the marine shell wastes by adopting mild water extraction, so that the obtained extract is rich in protein, polysaccharide or small molecule peptide, has good biological activity, and can be used for preparing functional materials such as biomimetic mineralization, tissue repair and the like; extracted shell powder residues are subjected to heat treatment or chemical activation treatment and then compounded with natural polymer materials such as chitosan and sodium alginate, and the composite adsorbent which is loose in structure and large in specific surface area is prepared and can be used for removing dye, heavy metal, antibiotics and other water pollutants. According to the invention, separate utilization of organic components and inorganic framework materials in the marine shells is realized, and the method has the characteristics of environmental protection, efficient resource conversion and multifunctional application, and has a good industrial popularization prospect.

The invention discloses a lytic bacteriophage for preventing and controlling vibrio in an aquaculture environment. The bacteriophage vBValMR42H belongs to a muscle tail bacteriophage and is insensitive to chloroform, the capsid of the bacteriophage vBValMR42H is free of lipid substances, and the preservation number is GDMCC No: 67434-B1. The bacteriophage has the characteristics of high adsorption speed, short incubation period and high cracking amount, keeps high activity in a wide range of temperature (4-55 DEG C) and pH (2-11), and has strong environmental adaptability. Genome analysis shows that the bacteriophage does not contain virulence factors, antibiotic resistance genes and lyogen related genes, and is high in biological safety. Physical development analysis shows that the method can be divided into a new genus. The bacteriophage R42H has the advantages of high splitting efficiency, good environmental stability, strong specificity, gene safety and the like, and can be used as an ideal biological prevention and control agent for preventing and controlling vibrio alginolyticus diseases in aquaculture.

The invention belongs to the technical field of wastewater treatment and recycling, and relates to a low-pressure ultrafiltration sewage treatment method and device based on bioelectrochemical enhancement and application. The treatment device consists of a water inlet tank, a constant water level water tank, a cathode, a resistor, a membrane assembly, a biological filter cake layer, a pre-filtering layer-anode, an ultrafiltration membrane, a water collecting device, a data acquisition module and a computer. The treatment device is used for treating effluent of a secondary sedimentation tank of a sewage plant and tail water of livestock and poultry breeding wastewater; on the basis of retaining nitrogen and phosphorus resources, deep removal of heavy metals and antibiotics is realized, propagation of resistance genes is inhibited, and safe reuse of nitrogen and phosphorus resources in effluent of sewage plants and tail water of livestock and poultry breeding wastewater can be realized. In the invention, low-pressure ultrafiltration adopts transmembrane pressure difference generated by gravitational potential energy to realize continuous flow filtration, coupled bioelectrochemistry utilizes electric energy generated by biodegradation of organic matters to realize reduction of heavy metals and deep removal of reinforced antibiotics, and the method has the advantages of greenness, low carbon and sustainability.

The invention relates to bacillus subtilis, the bacillus subtilis is named as bacillus subtilis BS02, the bacillus subtilis is preserved in the China Center for Type Culture Collection, the preservation date is April 11, 2025, and the preservation number is CCTCC NO: M 2025753. The bacillus subtilis can be used for preparing a bacillus subtilis strain. According to the technical scheme, the bacillus subtilis BS02 is provided and can replace antibiotics for livestock after being processed, the number of viable bacteria of intestinal escherichia coli is reduced, the number of viable bacteria of beneficial bacteria is increased, then the intestinal health effect can be improved, and the incidence rate and the fatality rate of diarrhea can be reduced; meanwhile, the bacillus subtilis BS02 can also improve the average daily gain of the piglets to a certain extent and reduce the feed conversion ratio, and has objective application value in the field of feed additives.

The invention discloses a 5 'UTR library, a recombinant expression vector and a genetically engineered bacterium for improving the yield of folamin in pathogenic bacterium Badei and application of the 5' UTR library and the genetically engineered bacterium, and belongs to the technical field of microbial genetic engineering. The method comprises the following steps: designing an SD region based on a 16S rRNA (ribosomal Ribonucleic Acid) 3'end complementary sequence of a Buddei pathogenic bacterium XBD101 serving as an original strain, regulating and controlling the number of bases between the SD sequence and an initiation codon AUG and optimizing an UTR secondary structure to construct a library containing 14 UTR sequences, and replacing three sequences N4A, N7A and N18A with the strongest translation ability in the library to the UTR region of a folamin synthesis key gene fclC, so as to obtain the folamin peptide. And finally, the capacity upgrading of the folamin chassis strain is realized, and an important support is provided for the industrial production of agricultural antibiotics.

The invention relates to a specific culture medium for detecting propionibacterium acnes in cosmetics and a detection method thereof, and belongs to the technical field of microbiological detection. Every 1000 mL of the specific culture medium contains 5-25 g of a nitrogen source, 5-10 g of a carbon source, 0.02-16 g of an inhibitor, 0.01-0.02 g of a color indicator, 0.1-13 g of a conditioning agent and the balance of a solvent. The inhibitor comprises Tween 80, a tea tree oil extract, cholate, sodium laurate and hydroxyproline. The specific culture medium provided by the invention is a propionibacterium acnes selective culture medium which is free of antibiotics, high in selectivity, sensitive in color development and low in cost. By optimizing the components of the culture medium and adding the selective inhibitor and the color indicator, the growth of other facultative anaerobic bacteria such as lactic acid bacteria can be effectively inhibited, specific culture and color development detection of propionibacterium acnes are carried out, and the accuracy and reliability of detection are improved.

The invention provides a modified biochar material embedded with electroactive bacteria as well as a preparation method and a use method of the modified biochar material. The preparation method comprises the following steps: S1, preparing modified biochar; s2, preparing an electroactive bacterium liquid, putting the modified biochar obtained in the step S1 into the electroactive bacterium liquid, and fully mixing, so that electroactive bacteria are uniformly adsorbed on the surface of the modified biochar; s3, adding the modified biochar uniformly adsorbed with the electroactive bacteria obtained in S2 into an embedding material solution, and fully and uniformly stirring to obtain a mixed solution; and S4, dropwise adding the mixed solution obtained in S3 into a cross-linking agent, so that the embedding material is cross-linked and cured on the surface of the modified biochar adsorbed with the electroactive bacteria, and washing and drying to obtain the modified biochar material embedded with the electroactive bacteria. The preparation method can provide a suitable growth environment and attachment surface for the electroactive bacteria, promote electron transfer between the electroactive bacteria and the external environment, accelerate the metabolic process of microorganisms, and further improve the degradation efficiency of pollutants such as antibiotics.

The invention relates to an mRNA (messenger Ribonucleic Acid) vaccine targeting FomA and application of the mRNA vaccine in immunotherapy of esophageal squamous carcinoma in the technical field of tumor immunotherapy. The problems that in the prior art, a specific targeting vaccine for the fusobacterium nucleatum membrane protein FomA lacks, traditional antibiotics can only kill extracellular bacteria and cannot remove intracellular colonized fusobacterium nucleatum, and cellular immunity cannot be effectively activated to remove fusobacterium nucleatum colonized in tumor tissue; the immunotherapy drug resistance of esophageal squamous carcinoma caused by fusobacterium nucleatum infection is difficult to reverse. According to the technical scheme, an mRNA molecule with a fusobacterium nucleatum FomA extracellular domain tandem repeat sequence and a lipid nanoparticle delivery system are coded, an extramembrane segment tandem repeat immune enhancement technology is adopted for design, two extracellular ring sequences are repeated for 2-5 times and connected through a connector sequence, lipid nanoparticles are composed of optimized quaternary lipids, and the lipid nanoparticles are prepared from the quaternary lipids. And a PD-1 monoclonal antibody is combined to activate FomA specific CD8 + T cell immunoreaction, so that comprehensive removal of intracellular and extracellular bacteria and bacteria targeted immune remodeling are realized.

The invention relates to the technical field of microorganisms, and particularly discloses a lactobacillus paracasei KQNIU, a microbial preparation and application of the lactobacillus paracasei KQNIU. The Lactobacillus paracasei KQNIU is preserved in the China General Microbiological Culture Collection Center on August 25, 2025, the preservation number is CGMCC No.35739, and the Lactobacillus paracasei KQNIU is classified and named as Lactobacillus paracasei, and the Lactobacillus paracasei KQNIU is named as Lactobacillus paracasei KQNIU. The Lactobacillus paracasei KQNIU is named as Lactobacillus paracasei KQNIU. The lactobacillus paracasei KQNIU provided by the invention has good gastrointestinal tract tolerance, has an inhibition effect on pathogenic bacteria such as escherichia coli, salmonella and the like, can be prepared into a microbial preparation or a feed additive for preventing and treating calf diarrhea, replaces antibiotics and realizes green and healthy breeding.

The invention relates to the field of adsorption materials, in particular to a discrete carbon-based biological base material for targeted treatment of high-concentration organic wastewater and a preparation process thereof.The discrete carbon-based biological base material is prepared from, by weight, 65-70 parts of activated carbon fibers, 8-10 parts of PLA fibers, 5-7 parts of sodium lignin sulfonate, 5-8 parts of ferroferric oxide magnetic particles and 8-10 parts of a carbon nitride catalyst; biPO4 is doped in the carbon nitride catalyst. According to the discrete carbon-based biological base material, the activated carbon fibers rapidly adsorb organic matters in high-concentration wastewater, the PLA releases a carbon source and accelerates formation of a biological membrane, and the biological membrane is degraded by taking the adsorbed organic matters as a substrate; the carbon nitride catalyst generates active oxygen under visible light to oxidize small molecular acid, and ferroferric oxide magnetic particles catalyze the reaction to break refractory toxins (such as antibiotics); after the reaction, the magnetic field recovers the material, sodium lignin sulfonate protects ferroferric oxide magnetic particles from corrosion, and regeneration performance is provided.

The invention discloses an escherichia coli-bacillus subtilis shuttle plasmid and application, and belongs to the field of gene engineering. The shuttle plasmid comprises the following elements: a promoter sequence, a coding sequence of bacillus subtilis signal peptide, a replication initiation sequence of bacillus subtilis, a replication initiation sequence of escherichia coli and an antibiotic resistance gene. According to the plasmid design, a modular structure is adopted, and quick replacement of key elements such as a promoter, a signal peptide and RBS is supported through optimized enzyme cutting site layout; a 6 * His tag introduced at the tail end of a multiple cloning site (MCS) can be adapted to nickel column affinity chromatography, and the purification process of the recombinant protein is simplified. The plasmid has the characteristics of small molecular weight and convenience in operation, can be efficiently cloned and constructed in escherichia coli and then directly converted into bacillus subtilis for protein expression, is particularly suitable for development of a gram-positive bacterium secretory expression system, and provides an efficient tool for large-scale production of industrial enzyme preparations.

The invention provides a compound microbial agent and application thereof in regulating aquaculture water quality, and the compound microbial agent is prepared by the following steps: firstly, embedding a bacterial liquid composed of alcaligenes faecalis, pseudomonas, ochrobactrum and sphingosine box bacteria through self-assembly by adopting gallic acyl quinic acid, and then modifying lignin by using ferrocene for composite loading. And immersing into a bacterial liquid consisting of photosynthetic bacteria, bacillus and nitrifying bacteria, and drying. According to the invention, ferrocene modified lignin and galloylquinic acid construct a multi-layer load structure to fix microorganisms in a layered manner, so that the microorganisms can stably survive in a complex water body environment, and meanwhile, a plurality of florae act synergistically to degrade organic matters, nitrogen, phosphorus, antibiotics and the like in aquaculture water, so that the degradation efficiency of water pollutants can be remarkably improved; effective adjustment of aquaculture water quality is facilitated, and good data support and theoretical basis are provided for water body improvement in the field of aquatic products.

The invention belongs to the technical field of microorganisms, and discloses a plesiomonas shigelloides bacteriophage with cross-genus splitting decomposition capacity and application of the plesiomonas shigelloides bacteriophage, the plesiomonas shigelloides bacteriophage is classified and named as Plesiomonas virus, the plesiomonas shigelloides bacteriophage is preserved in China General Microbiological Culture Collection Center on March 21, 2025, and the preservation number is CGMCC No.46409; the plesiomonas shigelloides bacteriophage has a splitting decomposition effect on both plesiomonas shigelloides and aeromonas veronii. The bacteriophage has a high cracking rate on plesiomonas shigelloides and also has a cracking effect on aeromonas veronii, is a bacteriophage with cross-genus-species cracking, and is good in alkali resistance stability and good in thermal stability. The compound can be prepared into drugs, environment disinfectants, water additives and the like for preventing and treating plesiomonas shigelloides diseases and / or aeromonas veronii diseases of cultured fishes, so that the use of antibiotics is reduced.

The present invention provides methods, compositions, and articles of manufacture useful for the prophylactic and therapeutic amelioration and treatment of gram-positive bacteria, and related conditions. The present invention provides compositions and methods incorporating and utilizing antibiotics represented by Formulae (I)-(IX) or derivatives or variants thereof.

The present invention relates to the early treatment, including pre-diagnosis treatment, of sepsis and acute inflammatory syndromes such as systemic inflammatory response syndrome (SIRS) by PLA2 and metalloprotease inhibitors to improve the performance of antibiotics and outcomes prior to and after confirmation of the diagnosis of sepsis and / or SIRS in a patient or subject. Additional embodiments include methods of treating sepsis, anthrax and severe acute respiratory syndrome coronavirus (SARS and SARS-CoV2) and related inflammatory syndromes and compositions, including pharmaceutical compositions and blood sample compositions. In further embodiments, the present invention is directed to embodiments which evidence that LY315920, LY333013 and related sPLA2 inhibitors are particularly effective COVID-19 / cytokine release syndrome therapeutics-prophylactics. In embodiments, the PLA2 inhibitor is varespladib (LY315920), methyl varespladib (LY333013), AZD2716-(R)-3-(5′-benzyl-2′-carbamoyl-[1,1′-biphenyl]-3-yl)-2-methylpropanoic acid—as a racemic mixture or separately, as the “R” enantiomer), compound 4 (3-(5′-benzyl-2′-carbamoyl-[1,1′-biphenyl]-3-yl)-propanoic acid) and LY433771 ((9-[(phenyl)methyl]-5-carbamoylcarbazol-4-yl) oxyacetic acid), a pharmaceutically acceptable salt thereof or a mixture thereof. In embodiments, the metalloprotease inhibitor is Prinomastat, Batimastat, marimastat or vorinostat dosed alone or in combination with preferred sPLA2 inhibitors for the treatment of infection, inflammatory and wound conditions arising from various causes. Methods and compositions for achieving accelerated treatment of wounds and burns, anthrax metalloprotease toxin (lethal factor) driven complications, ARDS, neo-natal and pediatric acute respiratory distress syndrome (neo-natal / pediatric ARDS), including meconium aspiration syndrome and other disease states and conditions are also disclosed.

The present invention discloses a recombinant Escherichia coli capable of efficiently producing monophospholipid A vaccine adjuvant, belonging to the fields of genetic engineering and synthetic biology. The present invention knocks out 132 genes related to membrane wall surface molecules, phospholipid and glucose transport, phosphoethanolamine modification and enoyl-ACP reductase in Escherichia coli MG1655, expresses lipid A modification genes FnlpxE, SepagP and SepagL, and constructs strain MW021 / pTEPL. The growth performance of MW021 / pTEPL is greatly improved and antibiotics and inducers are no longer required during the fermentation process; in a 2-L fermentation tank system, the highest OD of MW021 / pTEPL growth is 600 It can reach 38.54, and can produce 13.84g / L of dry cells and about 88.99mg / L of total lipid A; total lipid A contains only two structures, namely MPL and D-MPLA.

This invention discloses a hepatocyte model with silencing or overexpression of LCMT1 and its construction method, comprising: passage and seeding cells in logarithmic growth phase into well plates; diluting plasmid siRNA1 or siRNA2 or an overexpression plasmid to an appropriate concentration, and then mixing it with transfection reagent dilution to obtain a transfection mixture; washing the cells in each well of the plate and adding another culture medium, then gently dripping the transfection mixture into the well plate, shaking well, and culturing in a cell culture incubator for a period of time; replacing the culture medium with complete culture medium without antibiotics to obtain the constructed hepatocyte model with silencing or overexpression of LCMT1. This invention, by constructing a hepatocyte model with silencing or overexpression of LCMT1 in the liver, allows for validation experiments to be conducted to study the development mechanism of liver fibrosis. It is suitable for widespread application in various laboratories and provides an experimental model basis for research on liver fibrosis and drug trials.

The invention discloses a porous carbon composite material for degrading antibiotics through combination of photocatalysis and microorganisms and a preparation method of the porous carbon composite material, and belongs to a water treatment technology and catalyst development. Mixing and dispersing the titanium dioxide-porous carbon, a silane coupling agent, ferroferric oxide powder, deionized water and absolute ethyl alcohol to form dispersion liquid, and heating the dispersion liquid to react to obtain magnetized titanium dioxide-porous carbon; and carrying out mixed culture on the magnetized titanium dioxide and porous carbon and the bacterial suspension to obtain the porous carbon composite material. Titanium dioxide porous carbon obtained through modification and magnetic ferroferric oxide react through a silane coupling agent to obtain magnetized titanium dioxide porous carbon, and then bacterium suspension capable of degrading antibiotics and the magnetized titanium dioxide porous carbon are mixed and cultured to obtain the porous carbon composite material. The degradation capacity on antibiotics in the water body is improved, meanwhile, separation is easy, and secondary pollution to the water body is avoided.

The invention relates to the technical field of agricultural biology, and discloses a traditional Chinese medicine microbial probiotic stock solution, the active component of the stock solution comprises a fermentation product obtained by fermenting a traditional Chinese medicine fermentation substrate with a compound microbial flora, and the compound microbial flora comprises bacillus licheniformis, bacillus subtilis, saccharomyces cerevisiae and lactobacillus plantarum; the carbon and nitrogen source comprises 10-20 parts of brown sugar, 5-15 parts of bran and 1-3 parts of peptone, multiple strains are coordinated and complemented, the survival rate and colonization ability of the strains are improved by matching with the traditional Chinese medicine fermentation substrate, and the problem of unstable effect is solved; the composite carbon and nitrogen source and the functional traditional Chinese medicine group promote growth of multi-element metabolites, and insufficient efficacy is avoided; the fertilizer is suitable for breeding and planting multiple scenes, can replace antibiotics, and improves the fertilizer efficiency and delays the pesticide effect; the staged fermentation stimulates the synergism of strains, increases the active components, guarantees the stable efficiency, and assists the large-scale application of green agriculture.

This invention discloses an ecological water treatment process, comprising the following steps: treatment using a fluidized bed biofilm reactor; adding cellulose / sepiolite composite packing material and nutrient solution to the fluidized bed biofilm reactor; inoculating the fluidized bed biofilm reactor with activated sludge and performing an aeration reaction; operating the fluidized bed biofilm reactor with continuous ecological water intake, aeration reaction, and pH adjustment within the system; continuous sludge recirculation from the secondary sedimentation tank of the fluidized bed biofilm reactor during operation; when the water treatment packing material in the fluidized bed biofilm reactor reaches a certain thickness, adjusting the dissolved oxygen and pH within the system; continuous sludge recirculation from the secondary sedimentation tank of the fluidized bed biofilm reactor, and effluent. This treatment process can effectively degrade toxic organic substances in ecological water and exhibits excellent adsorption and high removal rates for antibiotic residues, achieving the purpose of purifying ecological water.

Provided herein are methods for identifying antimicrobial peptides deriving from extinct proteomes using a multitask deep learning approach. Also disclosed are antimicrobial peptides, and methods of treating an antimicrobial infection comprising contacting the infection with the present antimicrobial peptides. Also disclosed are methods of treating a microbial infection comprising administering to a subject in need thereof a pharmaceutically effective amount of an antimicrobial peptide according to the present disclosure.

The application discloses a preparation method and application of a polytriazimide / zinc ferrite photocatalytic composite material, and the composite material adopts a low-temperature molten salt method to prepare a high-crystallinity polytriazimide hexagonal prism as a structural reference; then zinc ferrite nanoparticles are in-situ grown on the surface of the hexagonal prism. The application in-situ grows visible light response semiconductor zinc ferrite nanoparticles on the surface of a polytriazimide hexagonal prism photocatalytic material for the first time; by constructing an organic / inorganic heterostructure, the defects of low utilization rate of visible light of the polytriazimide and acceleration of photogenerated carrier separation of a single-component material can be effectively solved, so that the overall photocatalytic activity of the composite material is improved. The photocatalytic composite material can be directly applied to removal of pathogenic microorganisms and organic antibiotic pollutants in water under visible light irradiation, and has great application prospect.

The present application belongs to the technical field of water pollution treatment, and particularly relates to a kind of constructed wetland coupled microbial fuel cell and a kind of water pollution treatment method.The modified iron-carbon material in the present application can reduce the inhibitory effect of antibiotics on device electricity generation, well maintain the activity of electricity-generating bacteria, and maintain stable electricity generation of the system;The modified iron-carbon material in the present application not only has efficient electron transport capacity, strengthens electron transfer, but also has a large number of active adsorption sites, can effectively remove ofloxacin, and is helpful for denitrification;At the same time, the surface area of the modified iron-carbon material is huge, and the surface is loaded with Fe, which can improve the removal capacity of phosphorus.The present application realizes efficient degradation of ofloxacin and conventional pollutants by reasonably constructing a constructed wetland coupled microbial fuel cell, and has higher electricity generation performance.

The application relates to the field of biotechnology, in particular to the application of Caenorhabditis elegans in constructing an animal model for detecting combined toxicity of antibiotics and metal ions. The application provides the application of Caenorhabditis elegans in constructing an animal model for detecting combined toxicity of antibiotics and metal ions. The application takes Caenorhabditis elegans as a model organism, and provides a simple, fast, low-cost, short-cycle and reproducible method for constructing an animal model for detecting combined toxicity. The application provides technical support for combined toxicity of antibiotics and metal ions in a natural environment and specific action aspects, and has practical application value.

Provided herein are hydrogels that include a plurality of amine-containing antimicrobial agents within the interiors of the hydrogels. The antimicrobial agents are connected to the hydrogel interiors via dynamic covalent imine bonds, and the hydrogel is engineered to facilitate controlled release of the antimicrobial agents. Also provided are methods for forming the provided hydrogels, and for using the hydrogels to treat a subject suffering from a microbial infection.

The utility model discloses a culture device of probiotics for replacement of antibiotics, which belongs to the technical field of culture of probiotics for replacement of antibiotics, and is characterized in that the culture device comprises a constant-temperature box, a warm lamp is arranged in the constant-temperature box, a storage mechanism is arranged in the constant-temperature box, the storage mechanism comprises a rotating shaft, the rotating shaft is rotatably connected with a sliding groove column, and the sliding groove column is connected with the warm lamp. A plurality of bearing pieces are connected to the sliding groove column in a sliding mode, each bearing piece comprises a sliding rod, one side of each sliding rod is connected with a culture dish in a sliding mode, the other side of each sliding rod is provided with a threaded rod, each threaded rod is connected with a T-shaped block, each T-shaped block is connected with the corresponding sliding rod in a sliding mode, and a pressing rod is connected to the side, away from the sliding groove column, of each threaded rod in a screwed mode. The device can ensure that other factors in the test are the same, and the reliability of the test result is ensured only by contrasting the warm light temperature intensity.