164 results about "Antibiotics" patented technology

The invention discloses a lytic bacteriophage for preventing and controlling vibrio in an aquaculture environment. The bacteriophage vBValMR42H belongs to a muscle tail bacteriophage and is insensitive to chloroform, the capsid of the bacteriophage vBValMR42H is free of lipid substances, and the preservation number is GDMCC No: 67434-B1. The bacteriophage has the characteristics of high adsorption speed, short incubation period and high cracking amount, keeps high activity in a wide range of temperature (4-55 DEG C) and pH (2-11), and has strong environmental adaptability. Genome analysis shows that the bacteriophage does not contain virulence factors, antibiotic resistance genes and lyogen related genes, and is high in biological safety. Physical development analysis shows that the method can be divided into a new genus. The bacteriophage R42H has the advantages of high splitting efficiency, good environmental stability, strong specificity, gene safety and the like, and can be used as an ideal biological prevention and control agent for preventing and controlling vibrio alginolyticus diseases in aquaculture.

The invention discloses a 5 'UTR library, a recombinant expression vector and a genetically engineered bacterium for improving the yield of folamin in pathogenic bacterium Badei and application of the 5' UTR library and the genetically engineered bacterium, and belongs to the technical field of microbial genetic engineering. The method comprises the following steps: designing an SD region based on a 16S rRNA (ribosomal Ribonucleic Acid) 3'end complementary sequence of a Buddei pathogenic bacterium XBD101 serving as an original strain, regulating and controlling the number of bases between the SD sequence and an initiation codon AUG and optimizing an UTR secondary structure to construct a library containing 14 UTR sequences, and replacing three sequences N4A, N7A and N18A with the strongest translation ability in the library to the UTR region of a folamin synthesis key gene fclC, so as to obtain the folamin peptide. And finally, the capacity upgrading of the folamin chassis strain is realized, and an important support is provided for the industrial production of agricultural antibiotics.

The invention relates to the technical field of microorganisms, and particularly discloses a lactobacillus paracasei KQNIU, a microbial preparation and application of the lactobacillus paracasei KQNIU. The Lactobacillus paracasei KQNIU is preserved in the China General Microbiological Culture Collection Center on August 25, 2025, the preservation number is CGMCC No.35739, and the Lactobacillus paracasei KQNIU is classified and named as Lactobacillus paracasei, and the Lactobacillus paracasei KQNIU is named as Lactobacillus paracasei KQNIU. The Lactobacillus paracasei KQNIU is named as Lactobacillus paracasei KQNIU. The lactobacillus paracasei KQNIU provided by the invention has good gastrointestinal tract tolerance, has an inhibition effect on pathogenic bacteria such as escherichia coli, salmonella and the like, can be prepared into a microbial preparation or a feed additive for preventing and treating calf diarrhea, replaces antibiotics and realizes green and healthy breeding.

The invention relates to the field of adsorption materials, in particular to a discrete carbon-based biological base material for targeted treatment of high-concentration organic wastewater and a preparation process thereof.The discrete carbon-based biological base material is prepared from, by weight, 65-70 parts of activated carbon fibers, 8-10 parts of PLA fibers, 5-7 parts of sodium lignin sulfonate, 5-8 parts of ferroferric oxide magnetic particles and 8-10 parts of a carbon nitride catalyst; biPO4 is doped in the carbon nitride catalyst. According to the discrete carbon-based biological base material, the activated carbon fibers rapidly adsorb organic matters in high-concentration wastewater, the PLA releases a carbon source and accelerates formation of a biological membrane, and the biological membrane is degraded by taking the adsorbed organic matters as a substrate; the carbon nitride catalyst generates active oxygen under visible light to oxidize small molecular acid, and ferroferric oxide magnetic particles catalyze the reaction to break refractory toxins (such as antibiotics); after the reaction, the magnetic field recovers the material, sodium lignin sulfonate protects ferroferric oxide magnetic particles from corrosion, and regeneration performance is provided.

The present invention relates to the early treatment, including pre-diagnosis treatment, of sepsis and acute inflammatory syndromes such as systemic inflammatory response syndrome (SIRS) by PLA2 and metalloprotease inhibitors to improve the performance of antibiotics and outcomes prior to and after confirmation of the diagnosis of sepsis and / or SIRS in a patient or subject. Additional embodiments include methods of treating sepsis, anthrax and severe acute respiratory syndrome coronavirus (SARS and SARS-CoV2) and related inflammatory syndromes and compositions, including pharmaceutical compositions and blood sample compositions. In further embodiments, the present invention is directed to embodiments which evidence that LY315920, LY333013 and related sPLA2 inhibitors are particularly effective COVID-19 / cytokine release syndrome therapeutics-prophylactics. In embodiments, the PLA2 inhibitor is varespladib (LY315920), methyl varespladib (LY333013), AZD2716-(R)-3-(5′-benzyl-2′-carbamoyl-[1,1′-biphenyl]-3-yl)-2-methylpropanoic acid—as a racemic mixture or separately, as the “R” enantiomer), compound 4 (3-(5′-benzyl-2′-carbamoyl-[1,1′-biphenyl]-3-yl)-propanoic acid) and LY433771 ((9-[(phenyl)methyl]-5-carbamoylcarbazol-4-yl) oxyacetic acid), a pharmaceutically acceptable salt thereof or a mixture thereof. In embodiments, the metalloprotease inhibitor is Prinomastat, Batimastat, marimastat or vorinostat dosed alone or in combination with preferred sPLA2 inhibitors for the treatment of infection, inflammatory and wound conditions arising from various causes. Methods and compositions for achieving accelerated treatment of wounds and burns, anthrax metalloprotease toxin (lethal factor) driven complications, ARDS, neo-natal and pediatric acute respiratory distress syndrome (neo-natal / pediatric ARDS), including meconium aspiration syndrome and other disease states and conditions are also disclosed.

This invention discloses a hepatocyte model with silencing or overexpression of LCMT1 and its construction method, comprising: passage and seeding cells in logarithmic growth phase into well plates; diluting plasmid siRNA1 or siRNA2 or an overexpression plasmid to an appropriate concentration, and then mixing it with transfection reagent dilution to obtain a transfection mixture; washing the cells in each well of the plate and adding another culture medium, then gently dripping the transfection mixture into the well plate, shaking well, and culturing in a cell culture incubator for a period of time; replacing the culture medium with complete culture medium without antibiotics to obtain the constructed hepatocyte model with silencing or overexpression of LCMT1. This invention, by constructing a hepatocyte model with silencing or overexpression of LCMT1 in the liver, allows for validation experiments to be conducted to study the development mechanism of liver fibrosis. It is suitable for widespread application in various laboratories and provides an experimental model basis for research on liver fibrosis and drug trials.

The invention relates to the technical field of agricultural biology, and discloses a traditional Chinese medicine microbial probiotic stock solution, the active component of the stock solution comprises a fermentation product obtained by fermenting a traditional Chinese medicine fermentation substrate with a compound microbial flora, and the compound microbial flora comprises bacillus licheniformis, bacillus subtilis, saccharomyces cerevisiae and lactobacillus plantarum; the carbon and nitrogen source comprises 10-20 parts of brown sugar, 5-15 parts of bran and 1-3 parts of peptone, multiple strains are coordinated and complemented, the survival rate and colonization ability of the strains are improved by matching with the traditional Chinese medicine fermentation substrate, and the problem of unstable effect is solved; the composite carbon and nitrogen source and the functional traditional Chinese medicine group promote growth of multi-element metabolites, and insufficient efficacy is avoided; the fertilizer is suitable for breeding and planting multiple scenes, can replace antibiotics, and improves the fertilizer efficiency and delays the pesticide effect; the staged fermentation stimulates the synergism of strains, increases the active components, guarantees the stable efficiency, and assists the large-scale application of green agriculture.

This invention discloses an ecological water treatment process, comprising the following steps: treatment using a fluidized bed biofilm reactor; adding cellulose / sepiolite composite packing material and nutrient solution to the fluidized bed biofilm reactor; inoculating the fluidized bed biofilm reactor with activated sludge and performing an aeration reaction; operating the fluidized bed biofilm reactor with continuous ecological water intake, aeration reaction, and pH adjustment within the system; continuous sludge recirculation from the secondary sedimentation tank of the fluidized bed biofilm reactor during operation; when the water treatment packing material in the fluidized bed biofilm reactor reaches a certain thickness, adjusting the dissolved oxygen and pH within the system; continuous sludge recirculation from the secondary sedimentation tank of the fluidized bed biofilm reactor, and effluent. This treatment process can effectively degrade toxic organic substances in ecological water and exhibits excellent adsorption and high removal rates for antibiotic residues, achieving the purpose of purifying ecological water.

The application discloses a preparation method and application of a polytriazimide / zinc ferrite photocatalytic composite material, and the composite material adopts a low-temperature molten salt method to prepare a high-crystallinity polytriazimide hexagonal prism as a structural reference; then zinc ferrite nanoparticles are in-situ grown on the surface of the hexagonal prism. The application in-situ grows visible light response semiconductor zinc ferrite nanoparticles on the surface of a polytriazimide hexagonal prism photocatalytic material for the first time; by constructing an organic / inorganic heterostructure, the defects of low utilization rate of visible light of the polytriazimide and acceleration of photogenerated carrier separation of a single-component material can be effectively solved, so that the overall photocatalytic activity of the composite material is improved. The photocatalytic composite material can be directly applied to removal of pathogenic microorganisms and organic antibiotic pollutants in water under visible light irradiation, and has great application prospect.

The present application belongs to the technical field of water pollution treatment, and particularly relates to a kind of constructed wetland coupled microbial fuel cell and a kind of water pollution treatment method.The modified iron-carbon material in the present application can reduce the inhibitory effect of antibiotics on device electricity generation, well maintain the activity of electricity-generating bacteria, and maintain stable electricity generation of the system;The modified iron-carbon material in the present application not only has efficient electron transport capacity, strengthens electron transfer, but also has a large number of active adsorption sites, can effectively remove ofloxacin, and is helpful for denitrification;At the same time, the surface area of the modified iron-carbon material is huge, and the surface is loaded with Fe, which can improve the removal capacity of phosphorus.The present application realizes efficient degradation of ofloxacin and conventional pollutants by reasonably constructing a constructed wetland coupled microbial fuel cell, and has higher electricity generation performance.

The present application relates to the field of biotechnology, and particularly relates to a KASP molecular marker related to the resistance of tilapia to streptococcus agalactiae disease and an application thereof.Two SNP sites (SNP-1 and SNP-2) significantly associated with the resistance are screened from the intron region of EGFRX2 gene of tilapia, a typing method of the above sites is developed through KASP technology, and the correlation between the SNP-1 genotype and the resistance phenotype is verified.Further, an advantage haplotype individual with the resistance to streptococcus agalactiae disease is screened by combining the two SNP sites, and the survival rate of the individual in the challenge experiment is significantly improved.The detection kit provided by the present application can realize high-throughput and high-precision typing, is suitable for the identification of the resistance genotype of tilapia and the marker-assisted selection, provides an efficient genetic improvement tool for the disease-resistant breeding of tilapia, and is helpful to reduce the loss of breeding diseases and reduce the dependence on antibiotics.

The application discloses a kind of fermented feed fermented by three strains of probiotics of Bacillus YA ( Bacillus sp ), yeast Y ( Saccharomyces cerevisiae ) and capsular red bacteria R3 ( Rhodobacter capsulatus ), and preparation method and application in aquaculture thereof, and viable count is not less than 1×10 7 CFU / g.The fermented feed is replaced ordinary feed according to certain proportion, and feeding result shows that aquaculture water quality is significantly improved after adding fermented feed, and fish growth performance, digestive enzyme activity, immunity, and muscle quality are also obviously improved.Using the feed in suitable range can shorten growth cycle, reduce cost, improve aquaculture environment, reduce antibiotic use, and improve muscle quality of farmed animals, with good economic and ecological benefits, simple fermentation process, low cost, and suitable for industrial large-scale production.

The present invention provides stable antimicrobial and disinfectant compositions comprising use of a solid precursor of an oxidized state of chlorine. The invention also provides on-demand storage and mixing vessels and methods for preparing and delivering on demand formulations. In addition, the invention provides antiviral, antibiotic and general antimicrobial uses, in vivo, on surfaces and via spray applications.

The application discloses a method for preparing porous zeolite composite materials from iron tailings and application, and converts fine-grained iron tailings with complex composition and low reaction activity into soluble salt through alkali fusion activation, and then converts the soluble salt into zeolite composite materials with uniform particle size and rich pores through hydrothermal crystallization. The application does not perform raw material pretreatment steps such as grinding and impurity removal, does not introduce a structure directing agent, controls the crystal structure of the iron tailings through alkali fusion activation-hydrothermal crystallization reaction, makes [SiO4] and [AlO6] structure units dissolve and recombine, simultaneously realizes in-situ doping and surface loading of iron elements, and constructs micrometer-level octahedral porous zeolite composite materials. The prepared porous zeolite composite materials have a large specific surface area, uniform pore size distribution and complete crystal structure, and exhibit excellent catalytic oxidation performance in a Fenton-like system, can efficiently remove emerging pollutants such as antibiotics, and are helpful to efficient and high-value utilization of fine-grained iron tailings resources, and have a wide application prospect in the field of wastewater treatment.

Helicobacter pylori (HP) is a chronic bacterium that infects the gastric mucosa. It is widespread in the general population, with an infection rate between 55% and 70%. HP is closely related to gastric ulcers and gastritis. Western medicine treatment for HP infection is relatively complex, including bismuth substituents and proton pump inhibitors plus two antibiotics. Gastric ulcers and gastritis are common digestive system diseases. Compound 1, "Exterminate the Ghost," is a traditional Chinese medicine composition mainly composed of Coptis chinensis, plus Scutellaria baicalensis and Fraxinus chinensis, which can eradicate HP. Compound 2, "Ulcer Relief," is a traditional Chinese medicine composition mainly composed of Coptis chinensis, plus Rheum palmatum and Bletilla striata, which can cure gastric ulcers. Compound 3, "Gasitis Relief," is a traditional Chinese medicine composition mainly composed of Coptis chinensis, plus Glycyrrhiza uralensis or Taraxacum mongolicum, made into a tea bag, which can cure chronic gastritis.

The invention discloses a preparation method and application of a photocatalytic membrane loaded on the basis of a charcoal-based g-C3N4 material. The invention relates to a preparation method of a photocatalytic membrane loaded on the basis of a charcoal-based gC3N4 material, which comprises the following steps: 1) dispersing PBC-gC3N4 in water, and carrying out ultrasonic treatment for 20-30 minutes; and (2) enabling the PBC-gC3N4 mixed aqueous solution in the step (1) to continuously pass through a PVDF (Polyvinylidene Fluoride) membrane with the pore diameter of 0.45 mu m by using a vacuum pump until the PBC-gC3N4 is firmly fixed on the surface of the PVDF membrane, and drying in a drying oven at 50-60 DEG C to obtain the photocatalytic membrane loaded with the biochar-based gC3N4 material. The photocatalytic membrane has adsorption and degradation capabilities and is applied to removal of pesticides and antibiotics.

This application relates to the field of environmental functional technology and discloses a magnetic porous chitosan-functionalized biochar and its preparation method. The material is prepared by solid-phase synthesis from a pre-carbonized biochar precursor, chitosan, organic acid iron salt, and sodium bicarbonate. The method includes: ball milling the raw materials to obtain an ultrafine composite powder, placing it in a constant temperature and humidity environment for moisture-induced solid-phase aging to allow the components to assemble at the interface; subsequently, subjecting it to restricted pyrolysis and in-situ magnetization under an inert atmosphere, and finally washing and drying to obtain the target product. This invention employs a solid-phase mechanochemical process combined with moisture aging, avoiding pore blockage caused by liquid-phase impregnation and inhibiting nitrogen loss during pyrolysis through coordination. The obtained product has a well-developed mesoporous structure, high nitrogen doping content, and rapid magnetic response characteristics, exhibiting excellent adsorption capacity, kinetic rate, and cycling stability for heavy metals and antibiotics.

The invention discloses a bacteriophage capable of splitting pathogenic escherichia coli and application of the bacteriophage. The phage is named as Escherichia coli phage vBEcoMYS1, and is preserved in the Guangdong Microbial Culture Collection Center, and the preservation number of the phage is GDMCC No: 67465-B1vBEcoMYS1. Experiments prove that the bacteriophage has relatively strong splitting capacity, relatively strong thermal stability and relatively strong pH stability, and can keep high titer under the conditions that the temperature is 4-40 DEG C and the pH is 5-9. The bacteriophage can be used for splitting multiple escherichia coli, including enterotoxigenic escherichia coli (ETEC) and shiga toxin-producing escherichia coli (STEC), has a relatively wide splitting spectrum, and can be used as an antibiotic substitute. In addition, the genome of the bacteriophage does not contain virulence genes and antibiotic resistance genes, so that the bacteriophage has good biological safety.

The invention belongs to the technical field of microorganisms, and particularly relates to pseudomonas aeruginosa and application thereof. According to the specific technical scheme, the pseudomonas aeruginosa HQ-2 is preserved in the Guangdong Microbial Culture Collection Center (GDMCC) on October 24, 2024, and the preservation number of the pseudomonas aeruginosa HQ-2 is GDMCC NO.65338. The pseudomonas aeruginosa HQ-2 is named as Pseudomonas aeruginosa HQ-2. The pseudomonas aeruginosa HQ-2 is named as Pseudomonas aeruginosa HQ-2. The pseudomonas aeruginosa HQ-2 is low in toxicity and sensitive to four indicating antibiotics, the yield of rhamnolipid is not reduced, and the pseudomonas aeruginosa HQ-2 is wide in application prospect.

The invention belongs to the technical field of microorganisms, and particularly discloses a bacillus aryabhattai strain and application thereof, the bacillus aryabhattai strain is identified as the bacillus aryabhattai strain through morphology, physiology, biochemistry and 16S rDNA (ribosomal deoxyribonucleic acid) molecules, is named as GR-6, is preserved in the China General Microbiological Culture Collection Center (CGMCC), and has the preservation number of CGMCC No.37494. The bacillus aryabhattai GR-6 disclosed by the invention has an in-vitro control effect of 67.58% and a living potting control effect of 38.67% on tomato gray leaf spot disease, and has good nitrogen fixation and potassium solubilization capabilities, and generated volatile substances have an obvious inhibition effect on various plant pathogenic fungi such as Stemphylium solani, Corynespora cassiicola, Botryis cinerea, fusarium oxysporum and Colletotrichum capsici; according to the present invention, the genome contains Surfactin, Macrolactin, Difficidin and other lipopeptide and polyketone antibiotic synthesis related genes, such that the biocontrol effect is significant, the growth promoting function and the broad-spectrum antibacterial ability are provided, the environmental protection is provided, the new microbial resource is provided for the green prevention and control of the tomato gray leaf spot disease, and the important application development value is provided.

The invention relates to the technical field of biochar materials, in particular to a soil carbon sequestration synergistic organic pollutant degradation method based on an iron-modified oxygen-carrying biochar material. According to the preparation method of the iron-modified oxygen-carrying biochar, iron modification and nano oxygen loading modification are combined, micropores of the prepared iron-modified oxygen-carrying biochar are obviously increased, the opening degree of the pores is increased, and the specific surface area and the number of surface active sites of the material are remarkably increased; the modified biochar material has very excellent performance in the aspects of promoting fixation of organic carbon in soil and adsorbing and degrading antibiotics, and has a relatively good application prospect in soil carbon fixation and emission reduction and pollutant degradation.

An AgCuTe nanowire composite material, its preparation method, and its application in medical antibacterial gauze are disclosed. The material consists of two phases: Cu7Te4 and Ag2Te, exhibiting a one-dimensional nanowire morphology with uniform silver distribution and nanowire diameters ranging from 7 to 9 nm. This material is prepared via a tellurium nanowire template combined with a two-step liquid-phase displacement reaction. First, tellurium nanowires are synthesized hydrothermally, followed by sequential reactions with copper and silver sources at room temperature to obtain the product. The AgCuTe nanowires exhibit concentration-dependent and power-dependent photothermal heating effects under 808 nm near-infrared light irradiation, with good photothermal stability. Under near-infrared light irradiation, the material demonstrates significant synergistic antibacterial activity against Staphylococcus aureus and Escherichia coli. Loading this material onto medical gauze can produce a near-infrared responsive photothermal antibacterial dressing for wound care and infection control, without antibiotic dependence, thus helping to reduce the risk of bacterial resistance.

The invention discloses a pseudomonas aeruginosa bacteriophage vBPaePpha0325 and an application of the pseudomonas aeruginosa bacteriophage vBPaePpha0325. The pseudomonas aeruginosa phage vBPaePpha0325 is classified and named as pseudomonas aeruginosa phage, and is preserved in the Guangdong Microbiological Culture Collection Center on December 29, 2025, the preservation number is GDMCC NO: 67562-B1, and the preservation address is Institute of Microbiology, Academy of Sciences, Guangdong Province. The bacteriophage vBPaePpha0325 shows remarkable splitting activity on pseudomonas aeruginosa, can efficiently kill and split host bacteria, and has the biological characteristics of short incubation period and high splitting quantity. The bacteriophage has strong adaptability to environmental conditions, and shows good temperature and acid-base tolerance. Genome analysis proves that the strain does not contain virulence genes and antibiotic resistance genes, has good biological safety, and shows application potential as an antibacterial preparation.

The invention relates to the field of microorganisms, in particular to cat-source lactobacillus plantarum capable of degrading oxalic acid and application of the cat-source lactobacillus plantarum, the preservation number of the cat-source lactobacillus plantarum is CGMCC NO.35212, and the cat-source lactobacillus plantarum is preserved in the China General Microbiological Culture Collection Center. The cat-derived lactobacillus plantarum has degradation capability on oxalic acid, has relatively strong acid resistance and cholate resistance, has a good inhibition effect on common pathogenic bacteria, is sensitive to various antibiotics and has good probiotic practical potential, and a new candidate strain is provided for pet food, health-care treatment products or probiotic preparations. In addition, the strain has a potential effect of preventing and treating calcium oxalate calculus, and has a wide prospect in the aspects of preparation of pet food, health care products, probiotic preparations, medicines and the like.

The present application belongs to the field of molecular biology detection, and particularly relates to a composition, method and use for simultaneous detection and differentiation of swine fever virus, psittacosis chlamydia, porcine reproductive and respiratory syndrome virus and porcine circovirus type II. The composition for simultaneous detection provided by the present application can realize rapid detection of four pathogen target genes in one tube of reaction solution by using multiplex fluorescence RPA detection technology, and has the advantages of high throughput, short time and low cost. On one hand, the present application can accurately detect pathogens and analyze mixed infection of pathogens, so as to achieve the effect of precise medication, reduce the use of antibiotics, save costs, and reduce the cost and increase the benefit of enterprises, and on the other hand, compared with fluorescence PCR, the detection time is greatly shortened, and the purpose of rapid detection is achieved.

The present application relates to the field of biotechnology, and more particularly to a detection method for resistance of tilapia to streptococcosis, a KASP primer set and application. The present application identifies a key SNP site of the ABCB4 gene significantly associated with resistance of tilapia to streptococcosis by the method of population genetics, and develops a genotyping method for the SNP site by KASP technology, thereby realizing efficient evaluation of the disease-resistant trait. Experimental data show that the survival time of AA genotype individuals in the challenge test is longer than that of GA / GG genotype individuals, the proportion of resistant fish is significantly improved, and the genotype frequency distribution is strongly correlated with the disease resistance of the population. The breeding strategy based on the SNP marker can improve the overall disease resistance of the breeding population and reduce the mortality rate, thereby providing an efficient genetic improvement tool for breeding of tilapia resistant to streptococcosis, and helping to reduce the loss of breeding diseases and reduce the dependence on antibiotics.

The invention belongs to the technical field of biology, and discloses a broad-spectrum avibacterium paragallinarum bacteriophage and application thereof. The avibacterium paragallinarum bacteriophage PIC25104 disclosed by the invention is separated from sewage collected from a farm, and the preservation number is CCTCC (China Center For Type Culture Collection) NO: M20253004. The bacteriophage can split avibacterium paragallinarum of different serotypes, the splitting rate is as high as 92.3%, and the bacteriophage can continuously inhibit bacteria for a long time, is high in stability and good in safety, and has good killing, prevention and control effects on the avibacterium paragallinarum in the environment and chickens. The bacteriophage can be used for developing a novel biological preparation to replace or supplement existing vaccines and antibiotics, fundamentally solves the problem of prevention and control of chicken infectious rhinitis, has urgent practical demands and great industrial value, and has a good prospect in clinical application of prevention and control of chicken infectious rhinitis.

The invention discloses a bacillus subtilis strain WN0101 and an application of the bacillus subtilis strain WN0101. The bacillus subtilis WN0101 is classified and named as bacillus subtilis, the bacillus subtilis WN0101 is preserved in the China Center for Type Culture Collection on September 29, 2025, and the preservation number of the bacillus subtilis WN0101 is CCTCC (China Center for Type Culture Collection) NO: M 20252145. The fermentation supernatant of the bacillus subtilis WN0101 can be used for specifically killing vibrio fluvialis and streptococcus iniae. In addition, the bacillus subtilis WN0101 has no hemolytic activity, and the fermentation supernatant of the bacillus subtilis WN0101 has good temperature stability and protease stability. Therefore, the bacillus subtilis WN0101 can replace antibiotics and is applied to prevention and treatment of vibrio fluvialis and streptococcus iniae infection.

The application discloses a novel bio-photoelectric coupling device, which comprises a shell, a water inlet formed in the bottom of the shell, and a water outlet formed in the top of the shell; an artificial wetland unit, which comprises a shunt layer and an intermediate substrate layer sequentially laid in the shell from bottom to top, and plants planted in the intermediate substrate layer; the water inlet is located at the bottom of one side of the shunt layer; a microbial fuel cell unit, which comprises an anode layer and a cathode layer, and the anode layer and the cathode layer are electrically connected through an external circuit, the anode layer is located between the shunt layer and the intermediate substrate layer, the cathode layer is located above the intermediate substrate layer, the top of the plants penetrates through the cathode layer and extends out of the shell, and the water outlet is located above the cathode layer. The coupling of the microbial fuel cell unit and the artificial wetland unit can not only remove common pollutants, but also remove composite pollutants such as antibiotics and heavy metal substances, improve the pollutant removal capacity, and realize more economical input and output.