421 results about "Antibiotics" patented technology

Disclosed is a novel Streptomyces sporosporus strain SC263 produced by UV mutagenesis, the produced antibiotics exhibit increased thermal stability, which exhibit antibiotic activity against bacteria and fungi. Antibiotics produced by SC263 and compositions thereof are also provided for the treatment of bacterial and fungal infections. The antibiotics have a very high molecular weight, which is suitable for topical administration.

The invention discloses an infection risk assessment method and system for burn nursing, and the system comprises a long-term trend analysis module and a short-term anomaly detection module, the long-term trend analysis module employs an improved Transform-XL model, and captures the chronic cumulative effect of inflammation indexes within 72 hours through a relative position coding and memory multiplexing mechanism; the short-term anomaly module is based on a bidirectional LSTM reconstruction network, combines a dynamic threshold algorithm to identify sudden physiological anomaly in a window of 6 hours in real time, generates a final infection risk index through a confidence-driven adaptive fusion algorithm and dynamic weighted dual-channel output, triggers clinical response in combination with a hierarchical early warning mechanism, and introduces closed-loop feedback optimization, so as to achieve the early warning of the physiological anomaly. The actual intervention effect feedback model is iterated, the infection recognition time is shortened to 3.2 hours, the ROC-AUC reaches 0.94, and the reasonable utilization rate of antibiotics is increased to 89%.

The invention discloses a method for traceless gene editing of gram negative bacteria, a carrier and application of the carrier, and belongs to the technical field of microbial genetic engineering. The invention provides a vector for performing traceless gene editing on bordetella pertussis. The vector simultaneously comprises an antibiotic positive screening gene expression box and a sacB and galk negative screening gene expression box. According to the method, the sacB gene and the galk genome are synthesized into the double-reverse-screening system for the first time, and compared with a single-reverse-screening system, the reverse-screening efficiency is remarkably improved, and the dosage of a reverse-screening reagent is remarkably reduced. The invention provides a novel gene editing method for non-mode bacteria such as pertussis bordetella and the like, and has a good application prospect.

The invention discloses a deep learning-based marine antibiotic pollution migration analysis method and system. The method comprises the steps of obtaining a target marine area water body image to form a first data set; according to the first data set, obtaining water body flow characteristics of the target ocean area, and analyzing a water body flow rule of the target ocean area by using an SARIMA model; the method comprises the following steps: acquiring driving factors influencing the flow change of a marine water body, performing driving force analysis by utilizing a Logistic regression model, constructing a state transition rule of water body flow, and constructing a pollution migration prediction model; obtaining water quality pollution detection information of each sampling point, carrying out pollution diffusion simulation based on a water body flow rule to obtain a pollution diffusion coefficient, and carrying out pollution migration prediction to obtain pollution migration prediction information; according to the pollution migration prediction information, the antibiotic pollution condition of the target ocean area is analyzed, the area needing antibiotic pollution treatment is analyzed, and an antibiotic pollution treatment scheme is generated. The prediction precision of pollutant migration is improved, and a scientific basis is provided for pollution prevention and control.

The invention belongs to the technical field of sewage treatment, and discloses a biodegradable-photocatalytic composite material as well as a preparation method and application thereof. The preparation method comprises the following steps: mixing a photocatalyst, sodium alginate and water to obtain gel containing the photocatalyst, then adding a photosynthetic bacteria concentrated solution, then adsorbing photosynthetic bacteria and the photocatalyst by using a carrier, and finally immobilizing in a calcium chloride solution to obtain the composite material. Photosynthetic bacteria are selected as microorganisms, and the method has the advantages of being flexible in metabolism mode, high in antibiotic tolerance, high in breeding speed, free of sludge treatment and the like. The photocatalyst and the photosynthetic bacteria are loaded on the polyurethane sponge, so that the photosynthetic bacteria are easily separated from water while being protected. The photocatalyst outside the carrier generates high-activity substances, and refractory antibiotics are converted into biodegradable intermediates. And a generated intermediate product is decomposed by microorganisms in the carrier, so that efficient degradation and mineralization of antibiotics in the sewage are realized.

The invention relates to a microbial agent prepared based on a new strain of bacillus velezensis, which has the function of broad-spectrum antagonism of common pathogenic bacteria of aquatic products and can effectively prevent and / or treat bacterial diseases and / or fungal diseases of aquatic organisms including crustaceans and fishes. Meanwhile, the content of nitrite and ammonia nitrogen in aquaculture water can be effectively reduced, and the usage amount and residual quantity of antibiotics and chemical drugs in aquaculture are reduced.

The invention discloses a high-valued graded utilization method of marine shells, and belongs to the technical field of comprehensive utilization of biological resources and preparation of functional materials. The method comprises the following steps: cleaning, air-drying and superfine grinding marine shell wastes such as abalone shells, and extracting water-soluble organic components in the marine shell wastes by adopting mild water extraction, so that the obtained extract is rich in protein, polysaccharide or small molecule peptide, has good biological activity, and can be used for preparing functional materials such as biomimetic mineralization, tissue repair and the like; extracted shell powder residues are subjected to heat treatment or chemical activation treatment and then compounded with natural polymer materials such as chitosan and sodium alginate, and the composite adsorbent which is loose in structure and large in specific surface area is prepared and can be used for removing dye, heavy metal, antibiotics and other water pollutants. According to the invention, separate utilization of organic components and inorganic framework materials in the marine shells is realized, and the method has the characteristics of environmental protection, efficient resource conversion and multifunctional application, and has a good industrial popularization prospect.

The invention discloses a lytic bacteriophage for preventing and controlling vibrio in an aquaculture environment. The bacteriophage vBValMR42H belongs to a muscle tail bacteriophage and is insensitive to chloroform, the capsid of the bacteriophage vBValMR42H is free of lipid substances, and the preservation number is GDMCC No: 67434-B1. The bacteriophage has the characteristics of high adsorption speed, short incubation period and high cracking amount, keeps high activity in a wide range of temperature (4-55 DEG C) and pH (2-11), and has strong environmental adaptability. Genome analysis shows that the bacteriophage does not contain virulence factors, antibiotic resistance genes and lyogen related genes, and is high in biological safety. Physical development analysis shows that the method can be divided into a new genus. The bacteriophage R42H has the advantages of high splitting efficiency, good environmental stability, strong specificity, gene safety and the like, and can be used as an ideal biological prevention and control agent for preventing and controlling vibrio alginolyticus diseases in aquaculture.

The invention relates to the field of biomedical materials, and discloses a preparation method and application of an antibacterial and anti-adhesion ultra-high molecular weight polyethylene fiber. According to the preparation method disclosed by the invention, the aminoglycoside antibiotics and the polysulfobetaine are stably modified on the surface of the UHMWPE fiber by utilizing the oxidation self-polymerization reaction of the polyphenol, and the UHMWPE fiber can be endowed with excellent antibacterial property and adhesion resistance by virtue of the broad-spectrum antibacterial property of the aminoglycoside antibiotics and the strong hydration effect of the polysulfobetaine; the application in the fields of antibacterial fabrics, biological materials and the like is facilitated.

The invention relates to an aerobic denitrification halomonas tianxiuensis LHT6, the preservation number of which is CGMCC No.32312. The strain can tolerate salinity, heavy metal ions and antibiotic stress under the condition of low carbon nitrogen ratio to realize efficient denitrification, and has application significance in actual treatment of water bodies such as aquaculture wastewater and pharmaceutical wastewater.

The invention belongs to the technical field of wastewater treatment and recycling, and relates to a low-pressure ultrafiltration sewage treatment method and device based on bioelectrochemical enhancement and application. The treatment device consists of a water inlet tank, a constant water level water tank, a cathode, a resistor, a membrane assembly, a biological filter cake layer, a pre-filtering layer-anode, an ultrafiltration membrane, a water collecting device, a data acquisition module and a computer. The treatment device is used for treating effluent of a secondary sedimentation tank of a sewage plant and tail water of livestock and poultry breeding wastewater; on the basis of retaining nitrogen and phosphorus resources, deep removal of heavy metals and antibiotics is realized, propagation of resistance genes is inhibited, and safe reuse of nitrogen and phosphorus resources in effluent of sewage plants and tail water of livestock and poultry breeding wastewater can be realized. In the invention, low-pressure ultrafiltration adopts transmembrane pressure difference generated by gravitational potential energy to realize continuous flow filtration, coupled bioelectrochemistry utilizes electric energy generated by biodegradation of organic matters to realize reduction of heavy metals and deep removal of reinforced antibiotics, and the method has the advantages of greenness, low carbon and sustainability.

Provided are compositions and methods for selectively reducing the amount of antibiotic resistant and / or virulent bacteria in a mixed bacteria population, or for reducing any other type of unwanted bacteria in a mixed bacteria population. The compositions and methods involve targeting bacteria that are differentiated from other members of the population by at least one unique clustered regularly interspaced short palindromic repeats (CRISPR) targeted DNA sequence. The compositions and methods can be readily adapted to target any bacteria or any bacteria plasmid, or both.

The invention relates to application of tryptophol in prevention and treatment of corn bacterial stem rot and relates to the technical field of biological prevention and control, the pathogenic bacterium of the corn bacterial stem rot is Dickeya zeae, it is found for the first time that tryptophol can inhibit the mobility of the Dickeya zeae, reduce the activity of cell wall degrading enzyme and relieve the disease degree, and the tryptophol can inhibit the activity of the Dickeya zeae, so that the tryptophol can be used for preventing and treating the corn bacterial stem rot. The tryptophol can effectively reduce the pathogenicity of tryptophol, but has no adverse effect on the cell viability and form of the corn dibasic bacteria, which indicates that the effect of tryptophol on the corn dibasic bacteria is realized by inhibiting the pathogenicity of bacteria instead of killing bacteria, so that tryptophol acts on the corn bacterial stem rot, the drug resistance is not easy to generate, and the tryptophol can be used for treating the corn bacterial stem rot. The tryptophol is expected to become a substitute of antibiotics and is used for preventing and treating the corn bacterial stem rot, the diseases can be effectively controlled, the selective pressure of drug resistance development can be relieved, and a new solution is provided for sustainable prevention and treatment of the corn bacterial stem rot.

The invention discloses a method and application for enhancing cytidine production, and belongs to the field of genetic engineering and microbial engineering. The present invention knocks out cytidine deaminase gene cdd, uridine kinase gene udk, pyrimidine-specific ribonucleoside hydrolase gene rihB, nucleotide 5'-monophosphate nucleosidase gene ppnN, pyruvate oxidase gene poxB and integrates and expresses 5'-CTP diphosphohydrolase, orotate phosphoribosyltransferase, uridylate kinase mutant gene PyrH (R92G / D93G), as well as phosphoribosyl pyrophosphate synthetase gene prs, glucose-6-phosphate dehydrogenase gene zwf and 6-phosphogluconate dehydrogenase gene gnd, and constructs a recombinant Escherichia coli with enhanced cytidine synthesis ability, and the cytidine yield reaches 61.9g / L in a 30L fermenter without adding antibiotics and inducers, which can be used to realize the industrial production of cytidine and realize green biomanufacturing.

The present application belongs to the field of microbial technology, and specifically discloses the application of Lactobacillus salivarius strain CGMCC No. 23518 in the preparation of antibacterial extracts. The present application inoculates Lactobacillus salivarius strain PC114 (CGMCC No. 23518) in the supernatant after fermentation in TPY medium, and obtains an antibacterial extract that inhibits intestinal pathogens, oral pathogens, epidermal pathogens, environmental pathogens and antibiotic-resistant microorganisms after ammonium sulfate precipitation, desalination and freeze-drying. The molecular weight of the effective ingredient of the antibacterial extract is greater than 3000Da, and it has no inhibitory effect on conventional probiotics. The present application discloses a new process and new use for preparing antibacterial substances by fermentation of Lactobacillus salivarius, and has a potential prospect for producing antibacterial substances.

The invention relates to bacillus subtilis, the bacillus subtilis is named as bacillus subtilis BS02, the bacillus subtilis is preserved in the China Center for Type Culture Collection, the preservation date is April 11, 2025, and the preservation number is CCTCC NO: M 2025753. The bacillus subtilis can be used for preparing a bacillus subtilis strain. According to the technical scheme, the bacillus subtilis BS02 is provided and can replace antibiotics for livestock after being processed, the number of viable bacteria of intestinal escherichia coli is reduced, the number of viable bacteria of beneficial bacteria is increased, then the intestinal health effect can be improved, and the incidence rate and the fatality rate of diarrhea can be reduced; meanwhile, the bacillus subtilis BS02 can also improve the average daily gain of the piglets to a certain extent and reduce the feed conversion ratio, and has objective application value in the field of feed additives.

The invention discloses a 5 'UTR library, a recombinant expression vector and a genetically engineered bacterium for improving the yield of folamin in pathogenic bacterium Badei and application of the 5' UTR library and the genetically engineered bacterium, and belongs to the technical field of microbial genetic engineering. The method comprises the following steps: designing an SD region based on a 16S rRNA (ribosomal Ribonucleic Acid) 3'end complementary sequence of a Buddei pathogenic bacterium XBD101 serving as an original strain, regulating and controlling the number of bases between the SD sequence and an initiation codon AUG and optimizing an UTR secondary structure to construct a library containing 14 UTR sequences, and replacing three sequences N4A, N7A and N18A with the strongest translation ability in the library to the UTR region of a folamin synthesis key gene fclC, so as to obtain the folamin peptide. And finally, the capacity upgrading of the folamin chassis strain is realized, and an important support is provided for the industrial production of agricultural antibiotics.

Provided are compositions and methods for selectively reducing the amount of antibiotic resistant and / or virulent bacteria in a mixed bacteria population, or for reducing any other type of unwanted bacteria in a mixed bacteria population. The compositions and methods involve targeting bacteria that are differentiated from other members of the population by at least one unique clustered regularly interspaced short palindromic repeats (CRISPR) targeted DNA sequence. The compositions and methods can be readily adapted to target any bacteria or any bacteria plasmid, or both.

The invention belongs to the technical field of fruit tree cultivation, and particularly relates to a nutritional bacterial fertilizer and application thereof in continuous cropping orchard planting. The nutritional bacterial fertilizer provided by the invention comprises a nitrogen fertilizer, a phosphorus fertilizer, a potassium fertilizer, a calcium fertilizer, an organic fertilizer, bacillus subtilis and trichoderma harzianum, and the organic fertilizer can improve the content of organic matters in soil; probiotic fungi (trichoderma harzianum) and probiotic bacteria (bacillus subtilis) are cooperatively applied, so that the absorption of mineral nutrition by the root system of the fruit tree can be promoted, and pathogenic fungi are prevented from infecting the root system of the fruit tree; meanwhile, antibiotic substances capable of inhibiting growth of bacteria, viruses, fungi and pathogens can be generated, and the effect of killing harmful microorganisms in soil is achieved; on the basis of optimizing the proportion of a conventional nitrogen-phosphorus-potassium formula, the calcium nutrition proportion is added, the formula is scientific and accurate, the main problems of serious physiological diseases, quality reduction and the like of apple fruits are solved, the utilization rate of tree body nutrients is remarkably increased, and the fruit quality is remarkably improved.

The invention belongs to the technical field of sewage purification treatment, and discloses an aquaculture wastewater ecological comprehensive treatment method and system.The method comprises the following steps that a plurality of hollowed-out composite filler balls prefabricated in a factory are placed in a sieve type purification filter dam assembling unit of an ecological purification filter dam; particles in aquaculture wastewater are filtered by filter holes with different diameters formed in a plurality of positions of a sieve type purification filter dam assembly unit, and meanwhile, when wastewater flows in, through iron-carbon micro-electrolysis coupling and an autotrophic-heterotrophic denitrification process of a solid-state carbon source and microorganisms, the particles in the aquaculture wastewater are filtered by the composite filler which is uniformly mixed and filled in a hollow composite filler ball according to a proportion. According to the aquaculture wastewater treatment system and the aquaculture wastewater treatment method, comprehensive ecological purification treatment including filtration, nitrification, denitrification, pollutant and harmful substance degradation and precipitation is performed on the wastewater, solid particles, total nitrogen, total phosphorus and antibiotics in the aquaculture wastewater are efficiently removed, up-to-standard discharge or cyclic utilization is realized, the treatment process and system are greatly simplified, and meanwhile, the construction and operation and maintenance cost of the whole system is also reduced.

The invention relates to a specific culture medium for detecting propionibacterium acnes in cosmetics and a detection method thereof, and belongs to the technical field of microbiological detection. Every 1000 mL of the specific culture medium contains 5-25 g of a nitrogen source, 5-10 g of a carbon source, 0.02-16 g of an inhibitor, 0.01-0.02 g of a color indicator, 0.1-13 g of a conditioning agent and the balance of a solvent. The inhibitor comprises Tween 80, a tea tree oil extract, cholate, sodium laurate and hydroxyproline. The specific culture medium provided by the invention is a propionibacterium acnes selective culture medium which is free of antibiotics, high in selectivity, sensitive in color development and low in cost. By optimizing the components of the culture medium and adding the selective inhibitor and the color indicator, the growth of other facultative anaerobic bacteria such as lactic acid bacteria can be effectively inhibited, specific culture and color development detection of propionibacterium acnes are carried out, and the accuracy and reliability of detection are improved.

The invention provides a modified biochar material embedded with electroactive bacteria as well as a preparation method and a use method of the modified biochar material. The preparation method comprises the following steps: S1, preparing modified biochar; s2, preparing an electroactive bacterium liquid, putting the modified biochar obtained in the step S1 into the electroactive bacterium liquid, and fully mixing, so that electroactive bacteria are uniformly adsorbed on the surface of the modified biochar; s3, adding the modified biochar uniformly adsorbed with the electroactive bacteria obtained in S2 into an embedding material solution, and fully and uniformly stirring to obtain a mixed solution; and S4, dropwise adding the mixed solution obtained in S3 into a cross-linking agent, so that the embedding material is cross-linked and cured on the surface of the modified biochar adsorbed with the electroactive bacteria, and washing and drying to obtain the modified biochar material embedded with the electroactive bacteria. The preparation method can provide a suitable growth environment and attachment surface for the electroactive bacteria, promote electron transfer between the electroactive bacteria and the external environment, accelerate the metabolic process of microorganisms, and further improve the degradation efficiency of pollutants such as antibiotics.

The invention discloses a strain for de novo synthesis of hydroxytyrosol and application of the strain, and belongs to the technical field of biology. The recombinant escherichia coli E.coli HT delta-glk provided by the invention can realize conversion of glucose and glycerol into hydroxytyrosol, the yield of produced hydroxytyrosol can reach 4.27 g / L, which is 3.62 times of the yield of hydroxytyrosol produced by YMGRH1D1 fermentation, and an inducer does not need to be added in the fermentation process; therefore, the raw materials are cheap and easy to obtain, additives, heavy metals and antibiotics which are harmful to human health are avoided, a good foundation is laid for later product purification, and the requirements of commercial application can be better met.

The invention relates to an mRNA (messenger Ribonucleic Acid) vaccine targeting FomA and application of the mRNA vaccine in immunotherapy of esophageal squamous carcinoma in the technical field of tumor immunotherapy. The problems that in the prior art, a specific targeting vaccine for the fusobacterium nucleatum membrane protein FomA lacks, traditional antibiotics can only kill extracellular bacteria and cannot remove intracellular colonized fusobacterium nucleatum, and cellular immunity cannot be effectively activated to remove fusobacterium nucleatum colonized in tumor tissue; the immunotherapy drug resistance of esophageal squamous carcinoma caused by fusobacterium nucleatum infection is difficult to reverse. According to the technical scheme, an mRNA molecule with a fusobacterium nucleatum FomA extracellular domain tandem repeat sequence and a lipid nanoparticle delivery system are coded, an extramembrane segment tandem repeat immune enhancement technology is adopted for design, two extracellular ring sequences are repeated for 2-5 times and connected through a connector sequence, lipid nanoparticles are composed of optimized quaternary lipids, and the lipid nanoparticles are prepared from the quaternary lipids. And a PD-1 monoclonal antibody is combined to activate FomA specific CD8 + T cell immunoreaction, so that comprehensive removal of intracellular and extracellular bacteria and bacteria targeted immune remodeling are realized.

The invention relates to the technical field of biology, in particular to a tilapia mossambica streptococcus agalactiae resistance related KASP molecular marker and application thereof. According to the invention, two SNP (Single Nucleotide Polymorphism) loci, namely SNP-1 and SNP-2, which are remarkably associated with resistance are screened based on an intron region of an EGFRX2 gene of tilapia mossambica, a typing method of the loci is developed through a KASP technology, and relevance between the genotype of the SNP-1 and a resistance phenotype is verified. Furthermore, streptococcus agalactiae resistance dominant diplotype individuals are screened in combination with double SNP loci, and the survival rate of a challenge experiment is remarkably improved. The detection kit provided by the invention can realize high-throughput and high-precision typing, is suitable for tilapia mossambica resistance genotype identification and molecular marker-assisted breeding, provides an efficient genetic improvement tool for tilapia mossambica disease-resistant breeding, and is beneficial to reduction of breeding disease loss and reduction of antibiotic dependence.

The invention relates to the technical field of microorganisms, and particularly discloses a lactobacillus paracasei KQNIU, a microbial preparation and application of the lactobacillus paracasei KQNIU. The Lactobacillus paracasei KQNIU is preserved in the China General Microbiological Culture Collection Center on August 25, 2025, the preservation number is CGMCC No.35739, and the Lactobacillus paracasei KQNIU is classified and named as Lactobacillus paracasei, and the Lactobacillus paracasei KQNIU is named as Lactobacillus paracasei KQNIU. The Lactobacillus paracasei KQNIU is named as Lactobacillus paracasei KQNIU. The lactobacillus paracasei KQNIU provided by the invention has good gastrointestinal tract tolerance, has an inhibition effect on pathogenic bacteria such as escherichia coli, salmonella and the like, can be prepared into a microbial preparation or a feed additive for preventing and treating calf diarrhea, replaces antibiotics and realizes green and healthy breeding.

Provided are compositions and methods for selectively reducing the amount of antibiotic resistant and / or virulent bacteria in a mixed bacteria population, or for reducing any other type of unwanted bacteria in a mixed bacteria population. The compositions and methods involve targeting bacteria that are differentiated from other members of the population by at least one unique clustered regularly interspaced short palindromic repeats (CRISPR) targeted DNA sequence. The compositions and methods can be readily adapted to target any bacteria or any bacteria plasmid, or both.

The invention discloses a method for degrading breeding manure antibiotics and synchronously preparing artificial soil by using coal gasification slag co-compost, and relates to a method for preparing artificial soil by using coal gasification slag co-compost. The invention aims to solve the technical problems of low utilization rate of existing coal gasification slag and residual antibiotics in livestock manure. According to the method, the coal gasification slag is added once during composting, the coal gasification slag is added once again after composting is completed, the artificial soil is prepared through mixing, the coal gasification slag is added in a large proportion by adding the coal gasification slag step by step, the composting temperature is increased, degradation of antibiotics is facilitated, and effective utilization of industrial waste is achieved; the chicken manure and the coal gasification slag are jointly fermented to prepare the artificial soil, so that a large amount of coal gasification slag can be consumed, the waste utilization rate is increased, the artificial soil which is stable in performance and capable of keeping an excellent application effect is prepared, and the problem of soil shortage in China is solved.

The invention relates to the field of adsorption materials, in particular to a discrete carbon-based biological base material for targeted treatment of high-concentration organic wastewater and a preparation process thereof.The discrete carbon-based biological base material is prepared from, by weight, 65-70 parts of activated carbon fibers, 8-10 parts of PLA fibers, 5-7 parts of sodium lignin sulfonate, 5-8 parts of ferroferric oxide magnetic particles and 8-10 parts of a carbon nitride catalyst; biPO4 is doped in the carbon nitride catalyst. According to the discrete carbon-based biological base material, the activated carbon fibers rapidly adsorb organic matters in high-concentration wastewater, the PLA releases a carbon source and accelerates formation of a biological membrane, and the biological membrane is degraded by taking the adsorbed organic matters as a substrate; the carbon nitride catalyst generates active oxygen under visible light to oxidize small molecular acid, and ferroferric oxide magnetic particles catalyze the reaction to break refractory toxins (such as antibiotics); after the reaction, the magnetic field recovers the material, sodium lignin sulfonate protects ferroferric oxide magnetic particles from corrosion, and regeneration performance is provided.

The invention discloses an escherichia coli-bacillus subtilis shuttle plasmid and application, and belongs to the field of gene engineering. The shuttle plasmid comprises the following elements: a promoter sequence, a coding sequence of bacillus subtilis signal peptide, a replication initiation sequence of bacillus subtilis, a replication initiation sequence of escherichia coli and an antibiotic resistance gene. According to the plasmid design, a modular structure is adopted, and quick replacement of key elements such as a promoter, a signal peptide and RBS is supported through optimized enzyme cutting site layout; a 6 * His tag introduced at the tail end of a multiple cloning site (MCS) can be adapted to nickel column affinity chromatography, and the purification process of the recombinant protein is simplified. The plasmid has the characteristics of small molecular weight and convenience in operation, can be efficiently cloned and constructed in escherichia coli and then directly converted into bacillus subtilis for protein expression, is particularly suitable for development of a gram-positive bacterium secretory expression system, and provides an efficient tool for large-scale production of industrial enzyme preparations.