84 results about "Pyricularia" patented technology

The present invention relates to an application of anthraquinone derivative as pesticice for controlling plant fungous diseases. The anthraquinone derivative pesticide contains one kind or several kinds of 10 anthraquinone derivative compounds. The anthraquinone derivative compounds have obvious action for preventing and curing several plant diseases, and can be used for preventing and curing economic crop, grain crop, vegetable, fruit tree and melon diseases due to fungi, including sphaerotheca, erysiphe, blumeria, fusarium, sclerotinia, pyricularia, rhizoctonia, colletotrichum, botrytis, verticillium and alternaria.

The invention discloses bacillus subtilis CYY-25 and belongs to the field of microorganisms. The bacillus subtilis CYY-25 is preserved with the preservation number CGMCC No.8995 in China General Microbiological Culture Collection Center and is capable of producing cellulase when salinity ranges from 0% to 15%. The bacillus subtilis CYY-25 is capable of synthetizing IAA and siderophore, dissolving sparingly soluble inorganic phosphorus, promoting plant growth and well inhibiting multiple kinds of plant pathogenic fungi such as fusarium oxysporum, pyricularia oryzae, sclerotinia sclerotiorum, fusarium moniliforme and sclerotinia sclerotiorum, is of great significance to prevention and control of alfalfa root rot and is suitable for being developed and used as an agricultural fungicide.

The invention discloses application of bacillus amyloliquefaciens in preventing and controlling fungal disease of plants. The application has the advantages that found by study, the bacillus amyloliquefaciens, especially bacillus amyloliquefaciens BSY3 separated and screened from African marigold by an inventor, has obvious antagonistic function on phellinus noxius, pyricularia cannaecola hashioka, sclerotinia sclerotiorum, colletotrichum sp., pestalotiopsissp, fusarium oxysporum f.sp.cubense and the like, and has obvious biological control function and potential application value on preventing and controlling of the fungi diseases due to pathogenic bacteria; the bacillus amyloliquefaciens comes from endophytic bacteria in plants, so that compared with the traditional chemical agent preventing and control method, the safer biological control effect on the fungi diseases is realized; because the bacillus amyloliquefaciens stays in the plant for a long time, the survival relationship with harmonious coexistence and stable development is established with the plant; compared with the chemical agent, the biocontrol function can be more effectively and stably realized.

The invention discloses a pyraclostrobin-containing agricultural bactericidal compound composition. The composition is compounded by active ingredients, namely pyraclostrobin (A) and a bactericide (B), wherein the bactericide (B) is any one of triflumizole, hymexazol and carbendazim; the weight ratio of the pyraclostrobin (A) to the bactericide (B) is (1:50)-(50:1); and the active ingredients account for 5 to 60 percent of the total weight of the agricultural bactericidal compound composition. The composition can be prepared into the conventional formulations such as water dispersible granules, wettable powder, a suspension, an oil-based suspension, microemulsion, emulsion in water, spreading oil, sustained-release granules and the like. The composition is used for controlling agricultural plant diseases, and is particularly used for controlling diseases such as powdery mildew, anthracnose and leaf spot disease of melons, vegetables and fruit trees, rice seedling blight, pyricularia oryzae, rice sheath blight disease and the like. The invention has the advantages that: the bactericide composition is efficient and has long duration, the environmental pollution is reduced, and the like.

The invention relates to burkholderia cepacia, and a cultivation method and an application thereof, and belongs to the field of plant protection (microbial pesticide). A preservation number of the burkholderia cepacia is CGCC No. 5384. According to the cultivation method, fermentation conditions are as follows: an LB culture medium is used; liquid volume is 50 mL; 1 mL of 2-day old seed strain is added; and the strain is cultured for 20-24 h at a temperature of 25-30 DEG C and at a rotation speed being 200 r/min. The burkholderia cepacia has significant inhibition effects for rhizoctonia solani, pyricularia oryzae, collectotrichum musae, fusarium oxysporum f. sp. cubense, gibberella zeae, peronophythora litchi, ceratocystis adiposum, fulvia fulva, xanthomonas oryzae pv. oryzae, xanthomonas oryzae pv. oryzicola and the like, can be used for biological control of plant pathogenic bacteria, has the advantages of low toxicity, easy degradation, environment friendliness and the like, and has wide application prospects.

The invention discloses a culture and preparation method for pyricularia oryza conidia. According to the method, the multi-starting-point culture technology and the aerial mycelium conidium production technology are utilized for fast culturing and preparing the pyricularia oryza conidia. The method comprises the following implementation steps that 1, a conidium production culture medium is prepared; 2, a multi-starting-point transplanting thallus is prepared; 3, the multi-starting-point transplanting thallus is planted in a culture plate; 4, the conidia are prepared and cultured; 5, the conidia are eluted and separated. The method has the advantages that fastness and high efficiency are achieved, and conidia of a new generation can reach the ideal state of a high sporulation quantity after five days of culture usually; sterile operation can be kept in the whole culture process, and the contamination probability of a finished conidium solution is greatly reduced; the operation technology is simple, and the result sporulation quantity is large.

The invention discloses a PCR detection and identification method capable of distinguishing pyricularia grise and magnaporthe oryzae and belongs to the technical field of plant fungus molecule biological detection. The PCR detection and identification method includes the steps of A, extracting DNA, to be specific, respectively extracting DNA of pyricularia grise and magnaporthe oryzae; B, designing primers, to be specific, designing a pair of specific primers Mol3-F/Mol3-F by inserting two ends of the retrotransposon of 849bp into a coding region of avirulence gene PWL3 of pyricularia grise; C, performing PCR, to be specific, performing PCR amplification by taking the extracted total DNA of pyricularia grise and magnaporthe oryzae as a template, so as to obtain an amplification product; D,determiningresults, to be specific, subjecting the amplification product to electrophoresis detection, if a target band of 1771bp is detected, then determining the product as pyricularia grise; if aband of 922bp is detected, then determining the product as magnaporthe oryzae. The PCR detection and identification method can rapidly accurately distinguish pyricularia grise and magnaporthe oryzae which are difficult to distinguish, and has a great significance in field spreading, breeding and evolving of Pyricularia on natural conditions.

The invention provides actinomycetes which is streptomyces samsunensis XJC-2-6, and the preservation number is CCTCC NO:M 2017620. The streptomyces samsunensis XJC-2-6 provided by the invention has anantagonism on fusarium oxysporum f.sp cubense No. 4, curvularia fallax, curvularia lunata, btoryosphaeria dothidea, colletotrichum acutatum, colletotrichum gloeosporioides, colletotrichum fragariae,botrytis cinerea, fusarium oxysporum (schl.) f.sp cucumerinum owen, colletotrichum acutatum, pyricularia oryae cav, fusahum graminearum sehw, botryosphaeria dothidea and the like, has wide developmentspace on prevention and treatment of Panama disease of banana, microscler streak disease of banana, large grey speck disease of banana, tree canker of banana, anthracnose of pepper, anthracnose caused by colletotrichum gloeosporioides, anthracnose of strawberry, disease caused by botrytis cinerea, fusarium wilt of cucumber, anthracnose of mango, rice blast, wheat scab, ring rot of apple and the like, and has very good development and application prospects.

The invention belongs to the technical field of pesticides, and particularly relates to a sterilizing composite containing tetramycin and prochloraz and application of the sterilizing composite in the aspect of controlling pyricularia oryzae. The sterilizing composite has the advantages that since the tetramycin and the prochloraz are adopted for compounding, the purpose of synergy is achieved, the controlling effect on the pyricularia oryzae is further improved, the used amount of drugs is reduced, the pesticide residue is reduced, and simultaneously the sterilizing composite plays a better role in delaying the drug resistance of pathogenic bacteria.

The present invention provides one new gene MgKIN17 from Pyricularia gisea, and the gene features that it has the amino acid sequence from the site 1 to the site 3544 in the SEQ ID No. 1, the cDNA with the nucleotide sequence of SEQ ID No. 2, the promoter with the nucleotide sequence of SEQ ID No. 4, and coded protein with the amino acid sequence of SEQ ID No. 3. The insertion mutation and gene complementation of the present invention shows that the destruction of the gene leads the formation of Pyricularia gisea infecting organ and reduced lesion capacity of rice. Wheat bakanae fungus, corn stalk rot and other pathogenic fungus have homogenous protein with homogeneity over 40 % to MgKIN17 and maybe similar biological function. The gene of the present invention and its protein expression and modification may be used as the target site for designing and screening antifungal medicines.

The invention discloses a pyricularia oryzae resistant oryza sativa gene OsWRKY47 and an application thereof. The gene is used for coding the protein with the amino acid sequence shown in SEQ IDNo:3 in a sequence table. The genomic DNA sequence and cDNA sequence of the gene are respectively shown in SEQ IDNo:1 and SEQ IDNo:2 in the sequence table. The gene OsWRKY47 has the function of improving the pyricularia oryzae resistance of the plants, is utilized to transform oryza sativa to obtain a disease resistant plant strain and enhances the resistance of oryza sativa to pyricularia oryzae.

The invention provides a method for selecting and breeding a new rice variety resistant to Pyricularia oryzae and Xanthomonas oryzae, and belongs to the technical field of rice disease resistance breeding. The method comprises the steps of selecting and using a two-line restoring line 9311 with a maximum promotion area in production as a to-be-improved parent, and performing hybridization with a Pyricularia oryzae and Xanthomonas oryzae resistant parent, backcrossing and selfing by utilizing a method of combining molecular marker-assisted selection with field agronomic selection; performing resistance identification on an obtained target gene homozygous line, and selecting and breeding three lines with constant agronomic traits and improved target resistance traits; and finally mixing the three lines by different ratios according to actual conditions of different planting areas to obtain the new variety with the diversified resistance, namely, "a super-resistant variety 9311". According to the method, the Pyricularia oryzae and Xanthomonas oryzae resistance of the rice variety with the diversified resistance is hugely improved on the basis of ensuring agronomic traits of an original background material, namely, the production increment and stabilization are facilitated, and the environmental protection is facilitated; and the method is of great significance for ensuring grain sustainable development.

The invention belongs to the field of heterosis utilization, and particularly discloses a method for producing hybrid rice seeds Chuanxiangzhan. The hybrid rice seeds Chuanxiangzhan are new three-line early rice compositions produced by hybridizing a cytoplasmic male sterile line Chuanxiang 29A and a restorer line Huazhan, wherein the female parent Chuanxiang 29A is a high-quality and pyricularia oryzae-resistant aromatic indica sterile line commonly bred by the crop research institute of Sichuan academy of agricultural sciences and Sichuan Huafeng seed industry Co., Ltd., and has good flowering habit and high outcrossing rate; and the male parent restorer line Huazhan is an inbred line. The hybrid seeds have the characteristics of high yield, stable production, high disease resistance and high biological yield.

An anti-phytopathogenic fungi activity psoralen flavanone relates to a compound, in particular to anti-phytopathogenic fungi activity psoralen flavanone. The preparation process of the compound comprises drying psoralen seeds, coldly soaking and extracting industrial alcohol four times, decompressing and condensing leachate to obtain alcohol extract, mixing solution with appropriate amount of water, extracting with petroleum ether and acetic acid ethyl ester in turn, taking acetic acid ethyl ester to extract position extract, conducting silica gel column chromatography, eluting in gradient with petroleum ether-acetic acid ethyl ester, separating to obtain psoralen flavanone (petroleum ether: acetic acid ethyl ester =85:15). The compound can be used as bactericide to prevent various plant diseases such as valsa mali, physalospora piricola nose, pyricularia oryzae, rhizoctonia solani AG-1 IA, cucumber fusarium oxysporum, gibberella zeae, cucumber anthracnose and phytophthora parasitica and the like.

This invention encompasses the identification and isolation of genes that confer disease control properties in plants, as well as plants comprising such genes. These genes are derived from the following sources: Nicotiana benthamiana, Oryzae sativa (var. Indica IR7), Papaver rhoeas, Saccharomyces cerevisiae and Trichoderma harzianum (Rifai 1295-22). The control conferred is against the one or more of the following phytopathogens: Aspergillus flavus, Cercospora zeae-maydis, Fusarium monilforme, Fusarium graminearum, Helminthosporium maydis, Phoma lingam, Phomopsis helianthi, Phytopthera infestans, Pyricularia oryzae, Pythium ultimum, Rhizoctonia solani, Sclerotinia sclerotiorum, Ustilago maydis, and Verticillium dahliae. Further, this invention encompasses other homologous and heterologous sequences with a high degree of functional similarity.

The invention relates to a pyricularia amide compound sterilization composition which belongs to a sterilization composition and comprises the effective gradients of pyricularia amide and prochloraz, wherein the mass ratio of the pyricularia amide to the prochloraz is 20: 1-1: 50, and the preferable mass ratio is 5: 1-1: 30. The invention has remarkable synergic effect after the pyricularia amide and the prochloraz are compounded, and is used for preventing and treating diseases, in particular to rice blast of crops, such as fruit trees, cottons, rice, vegetables, and the like.

The invention discloses stenotrophomonas rhizophila S11 capable of preventing and treating rice blast diseases of rice. The stenotrophomonas rhizophila S11 is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC No.18346. The invention discloses an application of the stenotrophomonas rhizophila S11 to preparation of medicines for preventingand treating the rice blast diseases of the rice. Besides, the invention discloses an antibiological inoculant capable of preventing and treating the rice blast diseases of the rice, a preparation method of the antibiological inoculant, and an application of the antibiological inoculant to prevention and treatment of the rice blast diseases of the rice. The antibiological inoculant contains the stenotrophomonas rhizophila S11. Through determination by a flat face-to-face culturing method, the inventor of the invention finds that the S11 strain has high antagonistic action on magnaporthe oryzae/pyricularia oryzae, and the diameter of an inhibition zone can reach 44mm; and green house inoculation and field experiment both indicate that the S11 antibiological inoculant can effectively prevent and treat the rice blast diseases of the rice. Therefore, the stenotrophomonas rhizophila S11 strain disclosed by the invention can be used for biological prevention and treatment of the rice blastdiseases of the rice, and has great popularization and application prospects.

The invention discloses a composite type biological pesticide fertilizer for preventing a rice disease and a preparation method thereof. The composite type biological pesticide fertilizer is composed of the following raw materials by weight percentage: 25-35% of any one of rapeseed cake or cotton seed cake, a peanut cake and tea fruit cake, 25-40% of fragrant eupatorium herb, 5-10% of goldthread, 5-15% of folium eucalypti, 5-15% of validamycin soluble powder, 3-8% of carbendazim, 3-8% of water-soluble boron, 5-10% of water-soluble zinc and 2-5% of copper sulfate solution. The preparation method comprises the following steps of: step 1, crushing any one of the rapeseed cake and the like into a cake; crushing the goldthread into grains; and cutting the fragrant eupatorium herb and the folium eucalypti into blocks or strips; step 2, mixing the cake with industrial acid and water to prepare an effective component extracting solution; step 3, respectively crushing the fragrant eupatorium herb, the goldthread and the folium eucalypti and adding the water to prepare the effective component extracting solution; and step 4, adding the effective component extracting solution, the validamycin soluble powder and the like into a reaction kettle to prepare a finished product. The product disclosed by the invention is green and environment-friendly and has higher fertilizer effect; and the composite type biological pesticide fertilizer has good disease-resistant sterilizing effects of Pyricularia oryzae, seedling blight and banded sclerotial blight.

The invention relates to a transplanting method of conidia of magnaporthe oryzae. The conidia are adsorbed from a spore-producing bacterial colony and are directly transplanted to an observation plate, and the operation steps of conidia transplanting are as follows: (1) preparing pathogen spores; (2) sterilizing tools; (3) preparing the observation plate; (4) adsorbing and diluting the spores; (5) releasing the spores; and 6) performing culturing and observing. The transplanting method has the advantages that (1) a key working material (the conidia of the magnaporthe oryzae) do not need to be made into a spore suspension, so that the pollution risk is reduced; (2) the conidia of the pyricularia oryzae can be dispersed on the strip-shaped culture plate, and the germination and growth activities of the conidia of the pyricularia oryzae are observed, so that the efficiency and the effect of observation work are improved, and development of multi-factor action design and batch test comparison in spore germination biological research is facilitated.

The invention discloses the avirulence gene AvrPi9 of rice blast fungus, the encoded polypeptide, polynucleotide and application thereof. The present invention provides the nucleotide sequence of a new avirulent gene Avr-Pi9 of the rice blast fungus (Magna-porthe grisea) and its encoded amino acid polypeptide sequence, and the gene is a gene whose expression is specifically induced when infecting plants. Based on this gene, transgenic plants with enhanced disease resistance can be prepared, or molecular markers for tracking changes in the population of M. oryzae can be prepared.

The invention discloses a bactericidal composition containing picarbutrazox and application of the bactericidal composition. Effective components comprise the picarbutrazox as a first active component and one of tricyclazole, fenoxanil, isoprothiolane and kasugamycin as a second active component, wherein the weight ratio of the first active component to the second active component is (1 to 80):(60 to 1), preferably (1 to 60):(40 to 1). The composition can be prepared into agriculturally-allowable dosage forms of wettable powder, a water dispersible granule, a suspending agent, suspension emulsion, emulsion in water, microemulsion, an aqueous capsule suspension, a microcapsule suspension-suspending agent, a dispersible oil suspending agent and dry suspension. The bactericidal composition disclosed by the invention is reasonable in composition and good in sterilization effect; in addition, the activity and the sterilization effect of the bactericidal composition are not simple superposition of the activities of all the components; compared with the conventional single preparation, the bactericidal composition has the advantages of obvious sterilization effect, remarkable synergistic effect and good safety to crops; the bactericidal composition disclosed by the invention has a remarkable prevention and control effect on pyricularia oryzae cav.

The invention discloses a pesticide for controlling pyricularia oryzae. The pesticide comprises kasugamycin and prochloraz in a mass ratio of (1-5):(1-5). According to the invention, after being compounded and mixed, kasugamycin and prochloraz have a synergistic effect, thus solving the problem of drug resistance of drug resistant pyricularia oryzae to single kasugamycin or prochloraz; the use amount of the pesticide can be effectively reduced; the labor intensity is lowered; the cost is saved; the problem of applying excessive pesticides is solved; and the pesticide is simple and practicable and has wide material source, low cost and good using effect.

The invention discloses a pesticide composition containing tetramycin and sedaxane. The pesticide composition comprises an inert ingredient, tetramycin and the sedaxane; the ratio of the parts by weight of the tetramycin to the sedaxane ranges from 1:1 to 1:80, and the total weight of the tetramycin and the sedaxane in the composition ranges from 1% to 80%; the tetramycin and the sedaxane have the obvious synergistic interaction effect on pathogen fungi after being compounded, and the pesticide composition is suitable for preventing and treating crop diseases caused by the pathogen fungi, and is particularly suitable for preventing and treating rice blast caused by pyricularia oryae.