58 results about "Pyricularia" patented technology

The invention belongs to the field of molecular biology, and particularly relates to application of OsDOF3 protein or a coding gene thereof to improvement of rice broad-spectrum disease resistance and regulation of agronomic traits. According to the application, an OsDOF3 knockout mutant and an overexpression transgenic line are constructed under the background of susceptible rice, resistance phenotype analysis is performed by inoculating various pathogenic bacteria (including pyricularia oryzae, rhizoctonia solani and bacterial blight), and it is proved that OsDOF3 participates in regulation and control of an immunoreaction pathway of rice to fungal diseases (rice blast and sheath blight) and bacterial diseases (bacterial blight). Meanwhile, the application also finds that after a strong promoter ubi is adopted for driving, an overexpression material of the OsDOF3 is shorter than that of a wild plant, and the OsDOF3 influences normal growth and development of rice while stimulating plant immune response. The invention provides important gene resources and molecular theoretical basis for developing a new variety of broad-spectrum disease-resistant rice.

The invention belongs to the field of microorganisms, and particularly relates to a banana endophyte compound fungicide as well as a preparation method and application thereof. According to the invention, a novel endophytic bacillus velezensis JDB15 strain and an endophytic trichoderma harzianum JDL4 strain are separated from a banana plant. The trichoderma harzianum JDL4 can inhibit botrytis cinerea, fusarium graminearum, rhizoctonia solani, peach anthracnose pathogen, peach brown rot pathogen and alternaria alternata, and has a good broad-spectrum antibacterial effect. Meanwhile, after the filtrate of the fermentation liquor of the trichoderma harzianum JDL4 and the bacillus velezensis JDB15 are compounded, the antibacterial effect is remarkably improved, and the control effect on banana fusarium wilt, pyricularia oryzae, tomato fusarium wilt and corn bipolaris maydis is also remarkably improved. The compound bacterial agent has wide biocontrol bacterial activity, can be developed deeply subsequently, and provides a sustainable and green solution for prevention and treatment of banana wilt and other plant diseases.

The invention belongs to the technical field of prevention and treatment of rice blast, and particularly relates to a polypeptide MP1 for inhibiting rice blast bacteria and application thereof. The invention discloses a polypeptide MP1 for inhibiting magnaporthe oryzae. The amino acid sequence of the polypeptide MP1 is as shown in SEQ ID NO. 1. The polypeptide MP1 has an obvious inhibition effect on the magnaporthe oryzae appressorium, and the EC50 formed by the MP1 polypeptide on the appressorium is 134.60 mu M. Experiments show that the polypeptide can effectively interfere the normal differentiation and development of the appressorium in the key pathogenic stage of magnaporthe oryzae, so that the polypeptide has a strong prevention and control potential in the early stage of pathogenic bacterium infection. The polypeptide MP1 provided by the invention can significantly reduce the pathogenicity of magnaporthe oryzae to barley and rice, and provides an experimental basis for developing a novel biopesticide for preventing and treating the magnaporthe oryzae.

The invention discloses an application of a small peptide GLP-1 (28-36) amide in preparation of a medicine for preventing and treating rice blast and a method of the small peptide GLP-1 (28-36) amide. The invention discloses an application of the small peptide GLP-1 (28-36) amide in preparation of a medicine for preventing and treating magnaporthe oryzae, and an application of the small peptide GLP-1 (28-36) amide in inhibition of magnaporthe oryzae and prevention and treatment of magnaporthe oryzae. The small peptide GLP-1 (28-36) amide can effectively inhibit pathogenicity of pyricularia oryzae spores in the concentration range of 12.5-100 [mu] M, especially the small peptide GLP-1 (28-36) amide with the concentration of 100 [mu] M can completely prevent and treat pyricularia oryzae, and the small peptide GLP-1 (28-36) amide can be used for developing novel green pesticides.

The application discloses application and a method of a small peptide GLP-1(28-36)amide in preparation of a medicine for preventing and treating rice blast. A scheme discloses application of the small peptide GLP-1(28-36)amide in preparation of a medicine for preventing and treating Pyricularia oryzae, and application of the provided small peptide GLP-1(28-36)amide in inhibiting Pyricularia oryzae and preventing and treating rice blast. The small peptide GLP-1(28-36)amide of the application can effectively inhibit pathogenicity of spores of Pyricularia oryzae in a concentration range of 12.5-100 muM, in particular, the small peptide GLP-1(28-36)amide with a concentration of 100 muM can completely prevent and treat rice blast, and can be used for developing a new type of green pesticide.

The present application provides a novel structure of thiophene compound, the compound has significant inhibitory effect on spore germination and appressorium formation of various plant pathogenic fungi and oomycetes (such as Pyricularia oryzae, Colletotrichum gloeosporioides, Gibberella, Phytophthora, Phytophthora capsici), which can be used for preventing and treating plant diseases caused by fungi and / or oomycetes.

The invention discloses application of a compound DBP-64 in preparation of a medicine for preventing and treating magnaporthe oryzae, belongs to the field of prevention and treatment of plant fungal diseases, and finds that the compound DBP-64 has a remarkable effect on inhibiting the magnaporthe oryzae or / and other pathogenic fungi, can effectively inhibit the pathogenicity of magnaporthe oryzae spores in a concentration range of 3-50 ppm, and can be used for preparing a medicine for preventing and treating the magnaporthe oryzae. Especially, 50 ppm of the compound DBP-64 can completely prevent and treat rice blast, and the application method is simple. The compound DBP-64 and a magnaporthe oryzae spore liquid are mixed and sprayed for 0 hour, so that a certain prevention and treatment effect is achieved, and the compound DBP-64 can be used as a protection medicament in the early stage of magnaporthe oryzae infection and has a better effect; moreover, the rice sprayed with the compound is good in growth situation and free of obvious adverse effects, which indicates that the compound is safe to the rice and has practical significance in preventing rice blast.

The application belongs to the field of agricultural fungicide compound, discloses a composition containing new white folium altheae alkaloid derivative Z-24 and anthraquinone components (emodin, emodin methyl ether, chrysophanol, aloe emodin) in rhubarb and mixed concentration thereof. The application of the composition in preventing and treating plant diseases caused by rhizoctonia solani, sclerotinia sclerotiorum, fusarium graminearum, botrytis cinerea, pyricularia oryzae and phytophthora capsici is disclosed, and the mass percentage of new white folium altheae alkaloid derivative Z-24 and anthraquinone components is 1:500, 1:100, 1:50 or 1:25. The fungicidal composition disclosed in the application has the advantages of wide fungicidal spectrum, low toxicity, effective delay of drug resistance of plant pathogens and the like, and has the value of further research and development.

The invention belongs to the technical field of pesticides, and relates to 11 bactericide compositions for mainly preventing and treating crop diseases caused by pyricularia oryzae and colletotrichum gloeosporioides. The compound YJY-22 provided by the invention is novel in structure and unique in action mode, and has no cross resistance with isoprothiolane, fenoxanil, pyraclostrobin, tricyclazole, azoxystrobin, mancozeb, captan, difenoconazole, prochloraz, chlorothalonil and tebuconazole. As the action mechanism of the compound YJY-22 is different from that of the medicament, and the compound YJY-22 and the medicament have no cross resistance, the compound YJY-22 and the medicament are compounded, so that the sterilization range can be expanded, the sterilization effect can be enhanced, the drug resistance of phytopathogen can be delayed, the safety to crops is good, the prevention and control spectrum is expanded, and the requirements of pesticide reduction and synergism are met.

The invention discloses application of pseudomonas aeruginosa T17 in prevention and treatment of rice bacterial blight. The invention discloses pseudomonas aeruginosa T17 and a function of a fermentation broth thereof as a biological agent in the aspect of disease control. Experiments prove that the pseudomonas aeruginosa T17 can directly inhibit growth of bacterial blight pathogenic bacteria (Xoo) and pyricularia oryzae (M.oryzae). Meanwhile, the fermentation product filtrate can directly induce up-regulated expression of rice defense related genes and up-regulated expression of jasmonic acid (JA) synthesis key genes OsAOS2, and the resistance of rice to bacterial blight can be enhanced by pretreating rice with the fermentation product filtrate. Therefore, the research lays a foundation for taking the pseudomonas aeruginosa T17 and the fermented filtrate thereof as the rice immune resistance inducer, and provides a new thought for developing innovative pollution-free botanical pesticides.

The invention discloses a solid fermentation method of a piriformospora indica solid fungicide. The solid fermentation method comprises the following steps: S1, activating a piriformospora indica strain; s2, inoculating a piriformospora indica strain into a solid fermentation culture medium, fully and uniformly mixing, adding sterile water to adjust the water content, and carrying out solid fermentation in a shady, cool and ventilated environment; wherein the solid fermentation medium is composed of a solid fermentation substrate, a carbon source, a nitrogen source and inorganic salt, the solid fermentation substrate selects crushed rape straw, the carbon source comprises glucose, cane sugar, corn starch and glycerin, the nitrogen source comprises peptone, urea, ammonium sulfate and potassium nitrate, and the inorganic salt comprises monopotassium phosphate, magnesium sulfate, sodium chloride and calcium sulfate. The water content of the solid fermentation culture medium is 40-70%, and the initial pH value is 4-7. The crushed rape straw is used as a fermentation substrate, an optimized nutrition formula and a one-step solid fermentation process are combined, efficient preparation of the microbial inoculum can be achieved, and the microbial inoculum is suitable for tobacco floating seedling raising and field transplanting application.

The invention discloses application of trans, cis-2, 6-nonadienal in prevention and treatment of rice diseases, and belongs to the technical field of plant disease prevention and treatment. Research finds that the trans, cis-2, 6-nonadienal as a plant self-synthesized substance has an obvious inhibition effect on the growth of xanthomonas oryzae and pyricularia grisea, and the trans, cis-2, 6-nonadienal can be used as an effective component to be applied to prevention and treatment of field bacterial streak and rice blast and improve plant immunity. The invention provides theoretical support for developing novel pesticides for preventing and treating rice bacterial streak and rice blast, and has important significance.

11 fungicide compositions mainly used for controlling crop diseases caused by Pyricularia oryzae and Colletotrichum spp.. Compound YJY-22 has a novel structure and a unique mode of action, and exhibits no cross-resistance with isoprothiolane, fenoxanil, pyraclostrobin, tricyclazole, azoxystrobin, mancozeb, captan, difenoconazole, prochloraz, chlorothalonil, or tebuconazole. Because compound YJY-22 and the described agents have different mechanisms of action and have no cross-resistance, combining compound YJY-22 and the described agents not only can broaden the antifungal spectrum and enhance antifungal efficacy, but also can delay fungicide resistance of plant pathogens, ensure high crop safety, expand the control spectrum, and meet the requirement of pesticide reduction with increased efficiency.

The application provides a pyroglutamic acid transaminase mutant and application thereof, the mutant is obtained by mutation on the basis of wild-type pyroglutamic acid transaminase, and specific mutation includes at least one of the following sites: (1) the 13th isoleucine is mutated into arginine; (2) the 35th valine is mutated into arginine; (3) the 183rd asparagine is mutated into aspartic acid; and the mutant is suitable for industrial enzymatic production of L-aspartic acid and the process of amino acids taking L-aspartic acid as a precursor; especially, the I13R / V35R / N183D mutant obtained by modification has high positive catalytic activity and strict direction selectivity, and completely loses the reverse reaction activity of decomposing L-aspartic acid; the mutant is overexpressed with phosphoenolpyruvate carboxylase in L-threonine production strain THRS-6, and a high-efficiency L-threonine synthesis metabolic pathway is constructed, and the yield of L-threonine is significantly improved when glucose is used as a substrate.

The invention relates to the technical field of pesticides for rice, and provides application of Chymostatin in prevention and control of rice blast in order to solve the problem that existing chemical pesticides cause harm to the ecological environment, and the application comprises the following steps: S1, inhibiting rice blast bacteria; s2, preventing and controlling rice blast; the CAS No of the Chymostatin is 9076 to 44 to 2, and the CAS No of the Chymostatin is 9076 to 44 to 2. Chymostatin has a very strong inhibition effect on pyricularia oryzae, is low in harm degree to animals such as human and livestock, is easy to degrade, is environment-friendly, and is beneficial to ecological balance and stability. The Chymostatin can obviously inhibit the formation of the magnaporthe oryzae appressoma so as to inhibit the infection of the magnaporthe oryzae appressoma. The compound has good inhibitory activity on various rice blast bacteria, and has broad spectrum.

The invention provides a preparation method and application of methyl 2-hydroxyphenylacetate. The methyl 2-hydroxyphenylacetate is separated from a fermentation culture of streptomyces wetland 13-3 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No. 22830. According to the method, the compound methyl 2-hydroxyphenylacetate is obtained from streptomycete for the first time, verification proves that the methyl 2-hydroxyphenylacetate has broad-spectrum antibacterial activity on various plant pathogenic fungi, particularly, the inhibitory activity on aspergillus fumigatus, colletotrichum truncatum, rhizoctonia solani, verruca verrucosa and magnaporthe oryzae is higher than that of amphotericin B, and the methyl 2-hydroxyphenylacetate can be used for preparing antibacterial agent drugs and has broad-spectrum antibacterial activity. A novel natural antibacterial compound is provided for the fields of agriculture and medicine, and the method is simple and convenient to operate, high in purification efficiency and environment-friendly.

The invention discloses an effect of an extracellular zinc metalloproteinase gene MoZMP1 in regulation and control of pathogenicity of magnaporthe oryzae. The full-length sequence of the MoZMP1 gene is as shown in SEQ ID NO.1, the cDNA sequence of the MoZMP1 gene is as shown in SEQ ID NO.2, and the amino acid sequence of the protein coded by the MoZMP1 gene is as shown in SEQ ID NO.3. According to the invention, a MoZMP1 gene is knocked out from magnaporthe oryzae, and a knockout mutant [delta] Mozmp1 is obtained. The analysis on the growth and development and pathogenicity of the knockout mutant delta Mozmp1 shows that the knockout of the MoZMP1 gene does not influence the growth and development process of the magnaporthe oryzae, but the pathogenicity of the magnaporthe oryzae is remarkably reduced, which indicates that the MoZMP1 gene can specifically regulate and control the virulence of the magnaporthe oryzae; the further analysis on the rice immunoreaction shows that a large amount of active oxygen and callose can be accumulated in the rice infected by the knockout mutant delta Mozmp1, and meanwhile, the expression of the rice defensive reaction gene is remarkably up-regulated, which indicates that the MoZMP1 gene has a remarkable effect on inhibiting the rice immunity and promoting the pathogenicity of the pyricularia oryzae.

This invention belongs to the field of bioengineering technology, specifically relating to a soluble powder of rice blast fungus immune protein and its preparation method. The powder mainly consists of rice blast fungus immune protein and specific carrier filler adjuvants. Through optimized formulation and processing technology, the problems of easy inactivation and difficult storage of protein-based active ingredients during industrialization are effectively solved, ultimately obtaining a soluble powder product with rapid dissolution, high suspension rate, and good stability. Field trials show that this formulation can efficiently activate the plant immune system, significantly control rice blast and sheath blight while reducing the amount of chemical pesticides used, and promote crop growth, resulting in increased yield and income.

The application discloses a benzisothiazole carboxamide compound and application thereof. The benzisothiazole carboxamide compound has the characteristics of high specificity and high activity as an inhibitor of myosin motor domain, and can significantly inhibit the growth and development process and pathogenicity of pathogenic fungi, especially Pyricularia oryzae.

The application discloses application of a broad-spectrum antibacterial biocontrol Cytospora fungus F-XTBG8 in preparation of antifungal active substances. Chaetomella raphigera The application of the broad-spectrum antibacterial biocontrol Cytospora fungus (F-XTBG8) is applied in preparation of antifungal active substances. The strain has obvious antagonism to more than 100 environmental fungi and plant pathogenic fungi including Pyricularia oryzae (Monascus oryzae), Mycosphaerella fijiensis (Mycosphaerella fijiensis), Alternaria tenuissima (Alternaria tenuissima), Fusarium graminearum (Fusarium graminearum), Fusarium oxysporum (Fusarium oxysporum) and Fusarium verticillioides (Fusarium verticillioides), and fermentation liquor of the strain can significantly reduce occurrence of corn large spot, tobacco black spot and sclerotinia rot. Magnaporthe oryzae Setosphaeria turcica Alternaria tenuissima Fusarium graminearum Fusarium oxysporum Fusarium concentricum The application provides an excellent biocontrol strain for development and research of microbial source pesticides and provides a new idea for green prevention and control of plant diseases.​​​​​

The invention relates to a method for rapidly and stably identifying rice blast resistance indoors and application, and belongs to the technical field of plant disease prevention and control. The invention provides a plum culture medium which takes water as a solvent and comprises 30-60 mL / L of prune juice, 3-10 g / L of alpha-lactose, 0.5-2 g / L of yeast extract and 10-20 g / L of agar powder. The pH value of the plum culture medium is 6.5 to 7.5. According to the inoculation method disclosed by the invention, the inoculation success rate and the scab appearing degree of the pyricularia oryzae are remarkably improved by optimizing the key steps of seed soaking and germination acceleration, strain activation and propagation, conidium suspension preparation, spray inoculation and the like, and the inoculation method has the advantages of low cost, simplicity and convenience in operation, high standardization degree, good inoculation effect, small batch difference and high repeatability.

The application discloses application of a compound DBP-64 in preparation of a medicine for preventing and treating Magnaporthe oryzae, and belongs to the field of plant fungal disease prevention and treatment. It is found that the compound DBP-64 has a remarkable effect in inhibiting Magnaporthe oryzae or / and other pathogenic fungi, can effectively inhibit the pathogenicity of Magnaporthe oryzae spores in a concentration range of 3-50 ppm, especially, the compound DBP-64 at 50 ppm can completely prevent and treat rice blast, and the application method is simple. The compound DBP-64 has a certain prevention and treatment effect when mixed with Magnaporthe oryzae spore liquid and sprayed at 0 h, indicating that the compound DBP-64 can be used as a protective agent at the initial stage of rice blast infection and has better effect; moreover, the sprayed compound has a good growth trend of rice and has no obvious adverse effect, indicating that the compound is safe to rice and has practical significance in preventing rice blast.

The invention belongs to the technical field of agricultural disease control, and particularly relates to an indole compound and application thereof. The structural formula of the indole compound is shown in the specification. The indole compound has the beneficial effects that the indole compound serving as an inhibitor of EMP1 has the characteristics of high specificity and high activity, and can remarkably inhibit the growth and development process and pathogenicity of pathogenic fungi (especially magnaporthe oryzae).

The application provides a Beauveria bassiana strain Bb-YY for preventing and controlling various diseases and pests, a derivative product and application thereof, and belongs to the technical field of functional microorganisms. Beauveria bassiana The strain Bb-YY is screened from the rhizosphere soil of a peanut healthy plant planted in a serious continuous cropping obstacle plot, and is identified as Beauveria bassiana (Bb) by molecular identification, has high efficient prevention and control effects on white grubs, peanut fruit rot pathogens and peanut root rot pathogens, and has certain inhibition effects on Bacterospora coriacea causing tobacco black foot disease and Pyricularia oryzae causing rice blast. It can be seen that the Bb-YY strain can also play a prevention and control effect on tobacco, rice and other crops in addition to peanut planting, and can effectively reduce the use of chemical pesticides, and provides a strong support for green prevention and control of peanut diseases and pests.

The invention relates to a triazole thioether compound containing a quinazoline structure as well as a preparation method and application of the triazole thioether compound, and relates to the technical field of chemical engineering and pesticides. According to the invention, 2-aminobenzoic acid and substituted benzoyl chloride are used as raw materials to synthesize a series of triazole thioether compounds containing a quinazoline structure; the prepared compound can treat plant bacterial diseases (rice bacterial leaf blight, rice bacterial leaf streak and citrus canker) or plant fungal diseases (cucumber botrytis cinerea, fusarium graminearum, eggplant verticillium wilt, pyricularia oryzae, potato late blight, rhizoctonia solani and the like). ) can be effectively prevented and controlled.

The application discloses application of a nano pesticide in inhibiting formation of appressorium of Pyricularia oryzae, wherein the nano pesticide is composed of copper ions and nano ZIF-8, and the copper ions are doped on the nano ZIF-8; wherein the mass ratio of the copper ions in the nano pesticide is 4.06 wt%. The nano pesticide has the advantages that not only can the formation of the appressorium of Pyricularia oryzae be inhibited, but also the production of active oxygen in mycelium can be induced, so that the morphological structure of the mycelium is caused to be oxidatively damaged; the nano pesticide mixed with conidia of Pyricularia oryzae is sprayed to inoculate rice leaves, the incidence of Pyricularia oryzae on the rice can be significantly reduced, and the prepared nano pesticide has no toxicity to the rice; Cu 2+ The copper ions doped into ZIF-8 and applied in inhibiting the formation of the appressorium of Pyricularia oryzae can not only improve the antibacterial activity of ZIF-8 on Pyricularia oryzae, but also reduce the pollution of traditional chemical pesticides to the environment.

The application provides an application of usnic acid or sodium usnate in prevention and treatment of wheat scab, and belongs to the technical field of pesticides.The natural product usnic acid and its sodium salt have inhibitory effects on plant pathogenic fungi Fusarium graminearum, Fusarium pseudograminearum, Pyricularia oryzae and Monographella nivalis, can significantly reduce the content of DON toxin of Fusarium graminearum, and have good inhibitory activity on wheat scab fungi in both indoor and field, thereby providing an important foundation for discovery and creation of new natural product pesticides.

The invention relates to a bacterial strain for antagonizing peach branch blight pathogen, a fungicide and application of the bacterial strain and the fungicide, and belongs to the technical field of agricultural microorganisms. The bacterial strain for antagonizing the peach branch blight pathogen is paenibacillus polymyxa JD9, and is preserved in the China General Microbiological Culture Collection Center on September 8, 2025, and the preservation number is CGMCC No. 35855. The JD9 strain is obtained by being separated from rhizosphere soil of a vegetable field, has a remarkable antagonistic effect on Diaporthe amygdali, and has broad-spectrum bacteriostatic activity on 10 plant pathogenic fungi such as peach monilinia, colletotrichum gloeosporioides and magnaporthe oryzae. The strain JD9 can be prepared into a microbial inoculum (the concentration is 1 * 10 < 8 > cfu / mL) through fermentation culture, is used for preventing and treating branch blight of peach fruits and branches, and has remarkable prevention and treatment effects. The biocontrol bacterium provided by the invention is green and safe, solves the problems of generation of drug resistance of germs and pesticide residues, environmental pollution and the like caused by excessive and nonstandard use of chemical agents, and has a good application prospect.

The invention relates to the technical field of antibacterial agents, in particular to an andrographolide derivative and a synthesis method and application thereof.The andrographolide is structurally modified, and a series of 14-site aryloxy / acylamino substituted derivatives are synthesized; the preparation method comprises the following steps: firstly, protecting 3, 19-dihydroxyl to obtain 3, 19-isopropylidene andrographolide; then, carrying out Mitsunobu reaction, so as to obtain a series of compounds of which the configuration at the C-14 site is reversed; and finally, in the presence of p-toluenesulfonic acid monohydrate, removing 3, 19-site protecting groups to obtain the target compound. The andrographolide derivative provided by the technical scheme has an inhibition rate of more than 60% on six test fungi at a concentration of 100 [mu] g / mL, and the activity of the andrographolide derivative is superior to that of a positive control drug azoxystrobin; and meanwhile, the hypha form of the pyrethrum can be obviously destroyed, and the vigna unguiculata seed germination is not influenced, so that high safety is shown.

The present application relates to the field of plant filamentous fungal disease prevention and treatment, in particular to the application of compound NSC-32 (N-[(5Z)-4-oxo-5-(phenylmethylidene)-2-sulfanylidene-1,3-thiazolidin-3-yl]pyridine-4-carboxamide) in the prevention and treatment of Magnaporthe oryzae. The present application takes MoPex19 protein as a target, screens compound NSC-32, and proves that the compound can inhibit the growth of Magnaporthe oryzae and reduce the pathogenicity of the latter. In addition, the present application also explores the applicable object, use method, target specificity and action mechanism of compound NSC-32, and provides a new green pesticide and new idea for the prevention and treatment of Magnaporthe oryzae.