48 results about "Mycosphaerella" patented technology

The invention discloses a bacillus belleus GT11 and application thereof. The bacillus belleus CT11 is collected at China General Microbiological Culture Collection Center on December 13th, 2016; the collection number is CGMCC No.13446; the bacillus belleus CT11 can be prepared into water agent, wettable powder or coating material and can be used for preventing and controlling the root rot diseases of pseudo-ginseng and plukenetia volubilis and Chinese cabbage clubroot. The invention provides an efficient, nontoxic, safe, non-residual and conveniently used biological agent for preventing and controlling the diseases such as root rot and clubroot; the effective active ingredient is bacillus belleus CT11 strain; the strain can generate the secondary metabolite antimicrobial active matter, is capable of degrading the cell wall of pathogenic bacteria and has an excellent growth-promoting effect for the crops, such as, plukenetia volubilis, pseudo-ginseng and Chinese cabbage; besides, the bacillus belleus CT11 has a better antibacterial effect for pseudo-ginseng phytophthora sojae, pseudo-ginseng fusarium solani, plukenetia volubilis gray mold, paddy bacterial mycosphaerella, and the like.

The invention belongs to the technical field of veterinary microbial additive preparation, and specifically relates to a strain of separated and screened Enterococcus faecium providing significant bacterial inhibition effects for common enteropathogenic bacteria such as staphylococcus aureus, escherichia coli and salmonella in breeding animals, and an application thereof. The probiotic Enterococcus faecium of the present invention is characterized in that: the strain is Enterococcus faecium HDRsEf1, and is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CCTCC NO:M2011031. The probiotic Enterococcus faecium of the present invention has characteristics of fast growth, high acid production capacity, strong stress resistance, safety, disease resistance and growth promotion, and can be used as the microbial feed additive for livestock and poultry feeds.

The invention discloses a pediococcus pentosaceus strain, ferment produced thereby and the use of ferment in meat product, wherein the docket number of the R9(Pediiococcus Pentosaceus I9) strain in the Chinese Microbiological Culture Preservation Administration Commission Common Microbiological Center is CGMCC No.0918. The leaven prepared from the bacterial can be applied into the fermentation production of meat food.

The invention relates to a salt-tolerant COD removal denitrifying microbial agent. The microbial agent consists of at least one of paracoccus sp FSTB-2, microbacterium kitamiense FSTB-4 and pseudomonas stutzeri FSTB-5, and further consists of at least one of paracoccus denitrificans DN-3 and methylobacterium phyllosphaerae SDN-3, wherein the paracoccus sp FSTB-2, the microbacterium kitamiense FSTB-4 and the pseudomonas stutzeri FSTB-5 are preserved in China General Microbiological Culture Collection Center on June 1, 2015, with preservation numbers of CGMCC No.10938, CGMCC No.10939 and CGMCC No.10940; and the paracoccus denitrificans DN-3 and the methylobacterium phyllosphaerae SDN-3 are disclosed in CN102465104A and CN102465103, and the preservation numbers of the paracoccus denitrificans DN-3 and the methylobacterium phyllosphaerae SDN-3 are CGMCC No.3658 and CGMCC No.3660. The microbial agent can be used for directly processing total nitrogen and COD in high-salinity wastewater, and the microbial agent can be also added to various biochemical reaction constructions so as to improve microbiological composition, optimize the salt tolerance of a microbial system in wastewater treatment and improve total nitrogen and COD removal rate of the entire process.

The invention relates to the technical field of biology and particularly relates to paracoccus denitrificans and an application thereof in treatment of livestock farm wastewater. The paracoccus denitrificans TDFQ-1 is collected at the China Center for Type Culture Collection (CCTCC), and the collection number is CCTCC NO:M2017238. The bacterial strain has the advantages of being high in growth speed, high in environmental tolerance and good in growth in an organic culture medium. The paracoccus denitrificans has a heterotrophic nitrification capability and an aerobic denitrification capability, and is capable of reducing harmful substances, such as ammonia nitrogen and nitrate in water, especially removing the ammonia nitrogen in the livestock farm wastewater with relatively high organic matter content and improving the breeding environment. The bacterial strain is capable of efficiently degrading fluoroquinolone antibiotics when treating the wastewater, reducing the pollution of the antibiotics to soil and water sources and protecting the human health.

The present invention relates to a new strain of coronavirus and its application. Said invention utilizes the throat-swabbed cell colture of SARS patient to separate out a new type coronavirus, called by name of SARS coronavirus Fudan I strain. By infecting Vero E 6 cell and patient's serum the indirect FA display is positive, after the influenza virus A and B, RS virus and pathogens of pneumococcus, etc. are excluded, the tests show that said separated coronavirus is a variant strain of new coronavirus. Said strain can be used for providing basis for preparing SARS diagnosis reagent and researchnig and producing medicine for resisting and curing SARS disease.

The invention relates to a pediococcus pentosaceus G11 strain as well as screening and applications of the pediococcus pentosaceus G11 strain. The pediococcus pentosaceus G11 strain is preserved in the China Center for Type Culture Collection, and is assigned with the accession number of CCTCC NO:2016248. The pediococcus pentosaceus G11 strain has tolerance to compound sulfamethoxazole and ceftazidime, has antibacterial activity for vibrio parahaemolyticus, aeromonas hydrophila, vibrio alginolyticus, staphylococcus aureus and beta streptococcus, has certain stress resistance and enzyme producing ability, is free of hemocyte solubility, and has no drug tolerance for most antibiotics; the dipping bath experiment proves that the pediococcus pentosaceus G11 strain is harmless to the host, can improve the immunity of the body, has wide application prospects, can be applied to probiotic preparations, and also can be prepared into bacterial powder which is then added into a feed as a feed additive, further, the growth of pathogenic bacteria in the bodies of the animals is inhibited, so that the death rate of animals is reduced, and the pediococcus pentosaceus G11 strain is a probiotic brain worthy of development.

The invention discloses an application of a distyrene-like compound containing thiazole ring as a sterilizing agent. A synthesized compound is subjected to antifungal activity testing as fusahum graminearum, mycosphaerella melonis, botrytis cinerea and thanatephorus cucumeris, testing structures can prove that (E)-4-(2,6-difluorophenyl)-2-substituted styryl thiazole compounds have certain inhibition activity for test targets, the inhibition rates of compounds Ic, If and Ii for fusahum graminearum are 50% or above, and the inhibition rates of compounds Id and Ii for mycosphaerella melonis are 55% or above, wherein the inhibition rate of the compound Id for mycosphaerella melonis is 61.6%, which shows medium inhibition activity.

The invention relates to a molecular marker method for a melon blight resistance gene, belonging to the field of biotechnology. There are four AFLP molecular markers that are linked to the gene for resistance to blight blight, the marker EcoRI-TG / MseI-CTC200 is a 200bp band; the marker EcoRI-AT / MseI-CTG90 is a 90bp band; the EcoRI-TC / MseI-CAG60 is a 60bp band band; the marker EcoRI-TG / MseI-CTA70 is a 70bp band; the linkage distances are 2.0, 6.0, 5.4 and 6.0cM, respectively. Detect whether PI 420145 and its derivative varieties (lines) contain the gene through the molecular marker method provided by the present invention, predict its resistance level to vine blight, improve the selection efficiency, and help to accelerate the selection of excellent vine blight-resistant melon varieties in my country It is used in the production process to identify the purity of muskmelon varieties resistant to vine blight.

The invention discloses Plectosphaerella sp. EF01 separated from a root tuber of radix tetrastigme and application of the Plectosphaerella sp. EF01 in promotion of growth of the radix tetrastigme and increasing of total flavonoid content. An endophytic fungus, namely the Plectosphaerella sp. EF01, is separated from the root tuber of the radix tetrastigme, the Plectosphaerella sp. EF01 has effects of promoting growth of the radix tetrastigme and increasing the total flavonoid content, the fresh weight of the plant is increased by above two times, the length of a root is above two times greater than that of a control group, expression quantity of Th-exp in leaves, stems and roots of the radix tetrastigme is increased, and the total flavonoid content is increased by 87-121%.

The invention relates to a genetically engineered bacterium with high-yield electroactivity and environmental stress tolerance. The lactic dehydrogenase gene ldhA in a pseudomonas aeruginosa PAO1 genome is knocked out, and the PAOl knockout strain is obtained; then a global regulatory factor IrrE of the deinococcus radiodurans is guided into the ldhA-, and the genetic engineering strain ldhA--irrE is obtained. After the thallus treated through a chemical agent is inoculated to a microbial fuel cell, the generated voltage and power density are improved by 46.76%-53.33% than those of the wild type strain, the time for stabilizing a system is shortened by 28.57%, the internal resistance of the system is reduced by 12.66%, the voltage under polyethylene glycol treatment is 543, the power density is 207, the stabilizing time is 130 h, the internal resistance reaches 420.32, and the survival rate of the engineered strain under the hunger condition, the high-acid-base condition and high-salt condition are improved by one time than those of the wild type strain.

The invention relates to the technical field of microorganisms and in particular relates to tetragenococcus halophilus. The tetragenococcus halophilus can be used for remarkably improving the contentof 4-vinylguaiacol in soy sauce or thick sauce, so that the aroma of the soy sauce is increased and the flavor and quality are improved. Therefore, the invention also relates to a method for preparingthe soy sauce or the thick sauce; the method comprises the step of adding the tetragenococcus halophilus provided by the invention before soy sauce mash is fermented or in a soy sauce mash fermentation process.

The invention discloses a soy sauce flavor Pixian bean paste production method based on 4-ethyl guaiacol fermentation intensification. The biological fungicide comprises yeast fungicide and tetragenococcus halophilus fungicide, wherein the yeast fungicide inoculates a 5Be' malt agar culture medium through zygosaccharomyces rouxii and intermediate aroma yeast to obtain fermentation broth; the tetragenococcus halophilus fungicide inoculates an improved mMRS culture medium to tetragenococcus halophilus to obtain the fermentation broth; then, porous starch, saccharose and glycerinum are added intothe fermentation broth to obtain corresponding fungicide. The yeast fungicide and the tetragenococcus halophilus fungicide are added into the Pixian bean paste in a normal after fermentation period to be subjected to joint fermentation, the content of 4-ethyl guaiacol can be improved by three times or more, the quality of a traditional Pixian bean paste product is comprehensively improved, and the traditional Pixian bean paste product is full of new life.

The invention relates to processes of culture of a Deinococcus or a related bacterium using an oxidizer in conditions suitable for allowing the growth of Deinococcus and decreasing the growth of at least one other microorganism.

The invention discovers that dap gene (DRB0118) in Deinococcus radiodurans R1 improves the resistance of prokaryotes and plants. The invention constructs a recombinant vector comprising the gene; and the recombinant vector is transferred into prokaryotic and eukaryotic host cells. Experiments prove that: after the dap gene (DRB0118) is expressed in prokaryotic host cells and tobacco, the salt resistance of the prokaryotic host cells and the tobacco can be improved.

The invention belongs to the field of sewage treatment, and relates to a microbial preparation for urban green land sewage treatment and a using method thereof.The preparation is prepared from mixed bacterium liquid and a carrier according to the weight ratio of 1:2, wherein the mixed bacterium liquid is prepared from actinomycetes, alcaligenes, bacillus pumilus, candida mycoderma bacteria, bacillus mucilaginosus, clostridium papyrosolvens and paracoccus denitrificans.According to the microbial preparation and the using method thereof, various bacterium strains which can form dominant bacterial communities are prepared into the efficient microbial preparation, and the sewage treatment effect is good.

The invention relates to a Pediococcus pentosaceus R1 strain with in-vitro antioxidation activity and application thereof as an antioxidant in fermented meat products, belonging to the technical field of microbes. The Pediococcus pentosaceus R1 is collected at China General Microbiological Culture Collection Center (CGMCC), wherein the culture collection number is CGMCC No.9171, the collection date is May 16th, 2014, and the classification designation is Pediococcus pentosaceus. The Pediococcus pentosaceus R1 has favorable antioxidation activity in in-vitro antioxidation tests; the complete cell and intracellular extract have high capacity for removing hydroxy free radicals, and can inhibit the lipids from peroxidation; and the cell surface substances have antioxidation activity. Therefore, the Pediococcus pentosaceus R1 can be used as an antioxidant in fermented meat products to postpone the product deterioration caused by oxidation in the fermentation and storage process, thereby reducing the consumption of the antioxidant.

The invention provides enterococcus faecium, a high-density fermentation method of enterococcus faecium, and a microecological preparation prepared by adopting enterococcus faecium, belonging to the technical field of culturing of lactic acid bacteria. An enterococcus faecium strain with the obvious probiotic property and stress resistance is screened and taken as a feed additive, and the enterococcus faecium strain can effectively replace part of antibiotics. The enterococcus faecium provided by the technical scheme is E13-5, and is assigned with the preservation number of CCTCC M2017191. Theenterococcus faecium provided by the invention can be used as the safe and efficient novel feed additive for replacing part of the antibiotics, and thus the demands of the modern feed industry and livestock breeding industry are satisfied.

The invention provides paracoccus denitrificans and method for biological denitrification with combination of mineralized bed and paracoccus denitrificans. The paracoccus denitrificans is autotrophicdenitrification paracoccus denitrificans TNS-1, mineralized garbage is selected as a main ingredient of a packed bed, waste is recycled, the biologic enhancement effect is adopted for total nitrogen removal, and the wastewater nitrogen removal method is environmentally friendly, free of secondary contamination, low in treatment cost, low in energy consumption and low in carbon.

The invention discloses a culture method of watermelon and melon. The method specifically includes the following steps of soaking plump watermelon and melon seeds in a saturated table salt water solution for 4-4.2 hours at the vacuum degree from -0.08 MPa to -0.085 MPa, scooping up the watermelon and melon seeds to be thoroughly flushed, taking garden soil where watermelon and melon is continuously planted for 2-5 years to serve as a seedling breeding substrate, sowing the watermelon and melon seeds in the seedling breeding substrate, thoroughly watering the seeds after sowing ends, inoculating young stems and leaves of watermelon and melon with melon type fusarium oxysporum through a needle point method three days after inoculation of mycosphaerella, and instantly and comprehensively spraying an allium caelureum extraction solution to the young stems and leaves of watermelon and melon 10-11 hours after inoculation. The cultured watermelon and melon is good in overall disease resistance, the later-period maintenance pesticide spraying frequency is greatly reduced, the damage of pesticides to fruits is reduced, and thus the healthiness degree is higher.

The invention relates to a method for screening mycosphaerella melonis-resistant IL 14 of cucumber-pickled cucumber introgression line. According to batch treatment of different concentrations, plants with good resistance are screened, and the method belongs to the field of biotechnology breeding. The method comprises the following steps: by taking wild species pickled cucumber as a female parent, cultivating cucumber 'Beijing jietou' as a male parent, and hybridizing to obtain hybrid F1; combining the hybrid F1 with a somaclone variation chromosome reduplication technology by virtue of embryo rescue, thereby obtaining a fertile inter-specific heterologous tetraploid new species which is named as Cucumis hytivus; and by taking the heterologous tetraploid new species Cucumis hytivus as a female parent, performing system backcrossing and self-cross selective breeding with the cultivated variety 'Beijing jietou', thereby obtaining 86 parts of cucumber inter-specific introgression line materials carried with wild species chromosome segments, and respectively numbering as IL1-IL86. The 86 parts of introgression line groups are screened by adopting the greenhouse seedling stage artificial inoculation identification technology, stable moderate resistance or more strains are obtained by virtue of inoculation treatment in spring and autumn, the inoculation treatment is performed by increasing the concentration of bacterium, and the mycosphaerella melonis resistance strain IL14 is screened.

The invention discloses a method for improving the quality of tobacco leaves by using blastococcus cavernae sp.. The strain is classified and named as Blastococcus cavernae sp., and is preserved in the China Center for Type Culture Collection CCTCC, and the preservation number is M2016739. Microorganism property analysis and 16S rDNA gene sequence analysis show that the strain is a novel bacterium. After an SYSUZL-2 agent is sprayed to tobacco leaves, and after alcoholization fermentation, the fragrance quality of the tobacco leaves is improved, the content of reduction sugar is increased, thecontents of proteins, cellulose, total nitrogen and nicotine are reduced, the ratios of sugar to nitrogen, sugar to nicotine and nitrogen to nicotine trend are increased, chemical components of the tobacco leaves are relatively well harmonized, and the sensory quality of the tobacco leaves is also remarkably improved.

The invention provides a novel neisseria gonorrhoeae liquid culture medium, and a method for applying the same to a drug sensitivity test of neisseria gonorrhoeae, and relates to the technical field of drug sensitivity detection. The novel neisseria gonorrhoeae liquid culture medium is prepared from beef infusion liquid, fetal bovine serum, peptone, agar, a growth additive and basic meat soup, sothat the neisseria gonorrhoeae is grown well in the culture medium and observation of a bacterial colony influencing the growth of the neisseria gonorrhoeae caused by pigments or turbidity phenomena does not exist; according to the detection method of the drug sensitivity of the neisseria gonorrhoeae, co-incubation of the neisseria gonorrhoeae cultured in the neisseria gonorrhoeae culture medium and medicines with different concentration is carried out, and the minimum inhibitory concentration of the medicines is determined according to the turbidity extent of the neisseria gonorrhoeae culturemedium to obtain the drug sensitivity of the neisseria gonorrhoeae; moreover, detection speed is high, and the technical problem that a detection method for quickly, efficiently and accurately detecting the neisseria gonorrhoeae drug sensitivity is lacked in the prior art is solved.

The invention relates to a Pediococcus pentosaceus R1 strain with in-vitro antioxidation activity and application thereof as an antioxidant in fermented meat products, belonging to the technical field of microbes. The Pediococcus pentosaceus R1 is collected at China General Microbiological Culture Collection Center (CGMCC), wherein the culture collection number is CGMCC No.9171, the collection date is May 16th, 2014, and the classification designation is Pediococcus pentosaceus. The Pediococcus pentosaceus R1 has favorable antioxidation activity in in-vitro antioxidation tests; the complete cell and intracellular extract have high capacity for removing hydroxy free radicals, and can inhibit the lipids from peroxidation; and the cell surface substances have antioxidation activity. Therefore, the Pediococcus pentosaceus R1 can be used as an antioxidant in fermented meat products to postpone the product deterioration caused by oxidation in the fermentation and storage process, thereby reducing the consumption of the antioxidant.

The invention provides a hybridoma cell strain Sino11A of a type 11A pneumococcal monoclonal antibody. The preservation number of the pneumococcal monoclonal antibody is CGMCC No.9234. The monoclonal antibody provided by the invention is good in specificity and high in level, can be stored for a long period of time, and can be applied to content detection of type 11A pneumococcal polysaccharide and type 11A polysaccharide in a pneumonia polysaccharide vaccine.

The invention relates to the field of desorption of pollutants in environment or food through microorganism, in particular to pediococcus acidilactici, with high adsorption capacity to cypermethrin, which is separated and screened out from homemade pickles in Sichuan traditional families. The pediococcus acidilactici capable of effectively absorbing cypermethrin, with the strain number being D15,is registered and preserved in China General Microbiological Culture Collection Center; the preservation number is CGMCC No.16133, and the preservation data is July 19, 2018. The pediococcus acidilactici can effectively absorb cypermethrin, and further has certain absorption effect on other pyrethroid pesticides. The pediococcus acidilactici D15 can be developed into corresponding biological agents to be used for the desorption of pyrethroid in environment or food; or the pediococcus acidilactici D15 can be developed into health food or medicine to reduce the harm to human exposure by the pyrethroid pesticides.