Plectosphaerella sp. EF01 separated from root tuber of radix tetrastigme and application of Plectosphaerella sp. EF01

A cloverleaf, non-integrity technology, applied in the field of strains for improving the growth and development of cloverleaf, can solve the problems of long time to form spherical tuberous roots and slow root development, and achieve the effects of increasing the content of total flavonoids and promoting growth.

Active Publication Date: 2017-04-26
HANGZHOU NORMAL UNIVERSITY
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  • Abstract
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Problems solved by technology

[0008] The purpose of the present invention is to provide a small incomplete globular shell fungus EF01 and its application in promoting the growth of clover and increasing the total flavonoid content; it can overcome the problems of long time for forming spherical tubers and slow development of tubers in the growth process of clover

Method used

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  • Plectosphaerella sp. EF01 separated from root tuber of radix tetrastigme and application of Plectosphaerella sp. EF01
  • Plectosphaerella sp. EF01 separated from root tuber of radix tetrastigme and application of Plectosphaerella sp. EF01
  • Plectosphaerella sp. EF01 separated from root tuber of radix tetrastigme and application of Plectosphaerella sp. EF01

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Embodiment 1

[0021] 1. Materials and methods

[0022] (1) Isolation and purification of endophytic fungi in root tubers

[0023] Wild clover collected in the mountainous area of ​​Jinyun County, Zhejiang Province, take a spherical tuber with a diameter of about 2cm, wash off the surface soil with tap water, soak it in detergent for 10 minutes, rinse it with running water, and soak the tuber in a diluted 1 times saturated sodium hypochlorite (Antiformin) solution for 30 minutes, and finally rinsed with sterile water 3 times. Cut into small pieces with a length and width of 3-5 mm, place them in PDA medium containing 50 mg / L Str (streptomycin) (200 g / L of potatoes (peeled and cut into pieces, boiled in water and then filtered through gauze to remove slag), glucose 20 g / L and agar 14g / L, the solvent is deionized water, pH7.0), the cut surface faces the culture medium, 4-6 pieces per dish. At the same time, collect the sterile water used to rinse the tubers for the last time and spread them ...

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Abstract

The invention discloses Plectosphaerella sp. EF01 separated from a root tuber of radix tetrastigme and application of the Plectosphaerella sp. EF01 in promotion of growth of the radix tetrastigme and increasing of total flavonoid content. An endophytic fungus, namely the Plectosphaerella sp. EF01, is separated from the root tuber of the radix tetrastigme, the Plectosphaerella sp. EF01 has effects of promoting growth of the radix tetrastigme and increasing the total flavonoid content, the fresh weight of the plant is increased by above two times, the length of a root is above two times greater than that of a control group, expression quantity of Th-exp in leaves, stems and roots of the radix tetrastigme is increased, and the total flavonoid content is increased by 87-121%.

Description

[0001] (1) Technical field [0002] The invention relates to a bacterial strain for improving the growth and development of cloverleaf, in particular to the application of Plectosphaerella sp. EF01 in promoting the growth of cloverleaf and increasing the total flavonoid content. [0003] (2) Background technology [0004] Clover (Tetrastigma hemsleyanum Diels&Gilg ex Diels) is a rare medicinal plant. It is mainly used as medicine with spherical roots. It contains flavonoids, flavonoids, vitamins, amino acids and other medicinal ingredients. It can clear away heat and detoxify, reduce inflammation and relieve pain. In recent years, it has been found that it has anti-tumor effect. Under natural growth conditions, the environmental conditions (such as: temperature, humidity and light conditions, etc.) are harsh, and the growth is slow. It usually takes 3-5 years to form spherical roots (Qian Lihua, Dai Danli, Jiang Huiyan, Lin Weihong. Endangered medicine Advances in research on ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01H4/00C12R1/645
CPCA01H4/001A01H4/008C12N1/145C12R2001/645
Inventor 向太和宋亚玲武盼李亚菲童霞秀郑亦芳茅佳能
Owner HANGZHOU NORMAL UNIVERSITY
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