32 results about "Fresh weight" patented technology

The application relates to the field of microbial technology and discloses a Pseudomonas asiatica HNHMPs-260 and application thereof. The strain is preserved in the Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC No: 66854. Fermentation supernatant of the strain can prevent and treat root-knot nematode disease of Hami melon caused by Meloidogyne javanica. Experiments show that the fermentation supernatant has strong toxic effects on second instar larvae of root-knot nematodes and can significantly inhibit the hatching of egg masses and egg grains. In addition, the fermentation supernatant has good thermal stability and storage stability, and still maintains more than 80% nematocidal activity after high-temperature treatment at 121 DEG C and long-term storage at 4 DEG C. Meanwhile, the fermentation supernatant of the strain can promote the growth of Hami melon plants, improve physiological indexes such as root length, stem length, fresh weight and dry weight, and has the application potential of being developed into efficient, environment-friendly and multifunctional biological pesticides.

This invention provides a two-stage cultivation method for Ophiopogon japonicus, belonging to the field of Ophiopogon japonicus planting technology. The two-stage cultivation method includes the following steps: dividing the planting field into a paddy field and an Ophiopogon japonicus seedling field, and planting rice and Ophiopogon japonicus seedlings together in the paddy field and the Ophiopogon japonicus seedling field respectively; after the rice harvest, planting the Ophiopogon japonicus seedlings in the planting field. This invention enables crop rotation of Ophiopogon japonicus and rice within the same planting field within one year, ensuring Ophiopogon japonicus production while increasing rice production for one season, solving the problem of competition for land between medicinal and grain crops, thus protecting both medicinal and grain crops. Compared with traditional cultivation methods, the cultivation method of this invention significantly improves indicators such as leaf length, root number, number of fruit roots per plant, fresh weight of stems and leaves, fresh weight of roots, fresh weight of Ophiopogon japonicus per plant, and yield per acre. The two-stage cultivation method of this invention results in high Ophiopogon japonicus yield and low cost, while also increasing rice production. Therefore, the net benefit of the two-stage cultivation method of this invention is far higher than that of traditional cultivation methods.

The application belongs to the technical field of vegetable cultivation, and particularly relates to a seed priming method for improving radish seedling emergence rate. The radish seeds are primed by using water extract of broccoli seeds, and are dried through variable-temperature drying, so that seed germination in the priming process is avoided, and indexes such as seedling emergence rate, seedling emergence time and seedling fresh weight are significantly improved, and the improvement effect is obviously better than that of water priming and polyethylene glycol priming. The problem of low radish seedling emergence rate is effectively solved.

The application provides a strain of protozoan nephrospora NJAU-HN1 and an application thereof, and belongs to the technical field of agricultural microorganisms. The nephrospora NJAU-HN1 has a preservation number of CCTCC NO: C2025172. The nephrospora NJAU-HN1 can directly and effectively inhibit the survival of root-knot nematodes. Application of the nephrospora NJAU-HN1 or a culture thereof can significantly promote the growth of melon plants, increase the fresh weight, dry weight and root length of the plants. The nephrospora NJAU-HN1 provided by the application provides valuable microbial resources for the development of a new microbial preparation for green prevention and control of melon root-knot nematode disease and crop health promotion, and has a good application prospect.

The application discloses a kind of for decomposing forest litter composite microbial inoculant, preparation method and its application method, it is related to litter decomposition technical field.The application includes a kind of for decomposing forest litter composite microbial inoculant preparation method, comprising the following steps: S1.to Phanerochaete chrysosporium, Trichoderma reesei and ropegillus catenulatum are respectively carried out directional metabolism preactivation;S2.the three preactivation wet bacteria bodies obtained in step S1 are mixed according to the fresh weight ratio of Phanerochaete chrysosporium:Trichoderma reesei:ropegillus catenulatum 3:2:1, obtain mixed bacteria body;S3.mixed bacteria body is mixed with the straw carrier that is preprocessed by steam explosion according to the mass ratio of bacteria body fresh weight:carrier dry weight 1:2, under the condition of temperature 28 DEG C, relative humidity 92~95%, stationary culture 72 hours, carry out phased micro-perturbation aeration, construct physical symbiosis;S4.the material obtained in step S3 is dried to 15% moisture content at 40 DEG C, obtain composite microbial inoculant;Efficient and stable decomposition of pine forest litter is realized.

This invention discloses the application of TaCYP51H37 protein and related biomaterials in regulating plant stem base rot resistance. This invention constructs a transgenic... TaCYP51H37 wheat and TaCYP51H37 Silent wheat, through identification of its resistance to stem base rot, was found to show that, compared with the recipient control, it was more susceptible to *Fusarium graminearum* infection. TaCYP51H37 The disease severity index of wheat was significantly reduced, while the aboveground length, aboveground fresh weight, and aboveground dry weight were all significantly increased. TaCYP51H37 The phenotype of silent wheat was the opposite, with a disease severity index significantly higher than that of the control plants, and significantly lower aboveground part length, aboveground fresh weight, and aboveground dry weight. This invention is the first to discover that the TaCYP51H37 protein positively regulates plant resistance to stem rot, which is of great significance and application value for improving plant disease resistance and cultivating new disease-resistant plant varieties.

The application belongs to the technical field of edible fungi, and particularly relates to a wild field collected broad-adaptability pilose fungus strain NHBZ3 and a separation and culture method, a liquid strain preparation method and application thereof. The pilose fungus strain is named pilose fungus (Lyophyllum decastes) NHBZ3, and is preserved in the China General Microbiological Culture Collection Center on August 21, 2025, with a preservation number of CGMCC No. 42085. The strain has outstanding broad adaptability and can be adapted to various cultivation raw materials and different climate regions in the north and the south. The commodity is unique, the cap is lace-shaped and has high recognition, and the fresh weight of a single fungus is increased by 50% to 108% compared with that of an ordinary strain, and the taste is excellent.

The application discloses a high-efficiency ammonia-assimilating Bacillus compostus and application thereof, and relates to the technical field of microorganisms. Bacillus stercoris The strain is QY6, which has been preserved in the China General Microbiological Culture Collection Center (No. 3, Beichen West Road, Chaoyang District, Beijing) on December 23, 2024, with a preservation number of CGMCC No. 33139. The strain or a biological bacterial agent prepared from the strain can be used for composting of organic solid waste, so that ammonia emission can be significantly reduced; the composting product can be applied to soil, so that the contents of soil available nitrogen, organic matter and humic acid can be significantly increased; and the product can be used for cultivation of Chinese cabbage, so that the fresh weight can be significantly increased and the content of soluble sugar can be increased. The application provides a high-efficiency nitrogen-preserving and decay-promoting microbial strain, which can be used in the fields of agricultural waste resource utilization, composting quality improvement and efficiency enhancement and agricultural planting.

The application discloses a plant salt-tolerance promoting active metabolite separated from a rhizosphere growth-promoting Pseudomonas proteus and application thereof. The metabolite is dibutyl phthalate (DBP) with a molecular formula of C 16 H 22 O4, has the effect of improving the salt tolerance of rice, and obviously increases the plant height, root length, aboveground fresh weight and underground fresh weight of the rice, is stable in effect, and has a good application prospect; and can be used as a preparation for improving the salt tolerance of crops in agricultural green production.

The application provides a peach PpNAC2 gene in improving plant cold resistance, belongs to the technical field of plant breeding. A peach PpNAC2 gene or a gene derivative product containing the peach PpNAC2 gene is applied in at least one of improving plant cold resistance, constructing cold-resistant transgenic plants and breeding cold-resistant plants. The application transforms the peach PpNAC2 gene into peach callus and Arabidopsis thaliana respectively under the mediation of Agrobacterium, and obtains transgenic materials. Low-temperature treatment (-4 DEG C) test finds that the fresh weight of the transgenic peach callus is obviously higher than that of the untransgenic peach callus, and the survival rate of the transgenic Arabidopsis thaliana is obviously higher than that of the wild type (WT). The peach PpNAC2 gene provides a candidate resource for plant cold-resistant breeding.

The present application discloses a strain of azotobacter chroococcum with the abilities of producing protease, decomposing potassium and cellulose, promoting growth and relieving plant NaCl stress, and application thereof. Azospirillum dongyingense The present application provides the azotobacter chroococcum or its offspring, the azotobacter chroococcum is azotobacter chroococcum (Azotobacter chroococcum) of Dongying, the strain number is RS252, and the registration number of the Center of General Microbiological Culture Collection of China Microbial Culture Collection Committee is CGMCC No. 31494. The strain is a new strain of azotobacter chroococcum genus, has the abilities of producing protease, decomposing potassium and cellulose. Potted test shows that compared with the negative control group without inoculation, the strain can improve the root length and underground fresh weight of rice under NaCl stress, and the strain can be used as microbial organic fertilizer to improve soil fertility, relieve crop NaCl stress and improve the adaptability of crops to NaCl stress.

This invention discloses a microbial compound inoculant and its application in blueberry growth, belonging to the field of microbial inoculant technology. The compound inoculant is composed of *Bacillus cereus*, *Pseudomonas koreensis*, and *Rhodococcus qingshengii* in a viable count-to-mass ratio of 1:1:1. The three strains exhibit significant synergistic effects, not only improving growth indicators such as stem length, root length, fresh weight, and dry weight of blueberries, but also increasing the content of soluble sugars, soluble proteins, and proline in blueberry leaves. The microbial compound inoculant constructed in this invention is adapted to the acidic growth environment of blueberries, is environmentally friendly, and low in cost, solving the problems of fertilizer dependence, slow growth, and lack of specialized inoculants in blueberry cultivation, and providing technical support for large-scale artificial cultivation of blueberries.

The invention discloses an application of a transcription factor MYB39 (Th4) in improving plant biomass, overexpression of the transcription factor MYB39 gene in corn can effectively improve the plant biomass and nitrogen utilization efficiency, and when transgenic corn is subjected to low-nitrogen treatment, the overground part fresh weight is improved by 31.5%, the underground part fresh weight is improved by 15.9%, and the total biomass is improved by 23.9%; during high nitrogen treatment, the fresh weight of the overground part is increased by 18.2%, the fresh weight of the underground part is increased by 2.2%, and the total biomass is increased by 11.3%, which indicates that the MYB39 gene has the effect of increasing the biomass of the corn plant and can be used for cultivating high-biomass plant varieties with high nitrogen efficiency.

This invention belongs to the field of green pest control technology in agriculture, and provides a method for controlling underground pests in peanuts: before crop sowing, previously planted Cnidium monnieri is pressed into the soil as green foliage; the sowing rate of Cnidium monnieri is 1-6.5 kg per mu, and the amount of green foliage returned to the soil is 7000-37500 kg / ha fresh weight, with the green foliage pressing occurring 5-10 days before crop sowing. The preferred underground pests are grubs, wireworms, and cutworms. This invention regulates the soil ecological environment through agronomic measures, which can increase the enrichment level of Metarhizium anisopliae in the soil and repel grubs, thereby reducing the occurrence of underground pests and mitigating yield losses caused by them. This method does not require the application of additional chemical agents specifically for underground pests and has good ecological and application value.

The application discloses a preparation method and application of PDRN (plant-derived RNA) from rice callus. The preparation method comprises the following steps: inducing and suspending culturing of the rice callus; homogenizing the rice callus suspension cells obtained in step 1; sequentially using cellulase, pectinase and protease K for enzymolysis; nucleic acid purification and precise ultrasonic shearing are performed in sequence, so that PDRN with a molecular weight of 25-500 bp is obtained. Through optimization of the rice callus culture condition and combination of the joint lysis process of "mechanical grinding-composite enzymolysis-protease treatment", efficient and high-purity preparation of the plant source PDRN is realized, the extraction rate can be higher than 0.5 mg / g of fresh weight of the callus, and the purity A260 / A280 is greater than or equal to 1.8. The obtained PDRN has significant effects in terms of anti-wrinkle, soothing, moisturizing, anti-inflammatory and anti-aging.

The present application relates to the field of traditional Chinese medicinal material cultivation and enzyme engineering technology, and particularly relates to a kind of ophiopogon japonicus control boom regulator based on carbon-based ecological fertilizer load composite enzyme preparation and its preparation method and application.The regulator comprises the following components in mass fraction:80-95 parts of functional liquid carbon-based ecological fertilizer matrix, 3-15 parts of targeted composite enzyme preparation, 1-3 parts of enzyme stabilizing protection system and 1-2 parts of cofactor activation liquid.The regulator can significantly increase the fresh weight and dry weight of ophiopogon japonicus tuber, the total saponin content and the crude polysaccharide content, has a significant control boom effect, has multiple functions of yield increase, quality improvement and soil improvement, and is suitable for green, efficient and sustainable cultivation of traditional Chinese medicinal materials.

The application belongs to the technical field of plant genetic engineering, and particularly relates to TaSIZ1 protein, TaSIZ1 gene and application of related biological materials of the TaSIZ1 gene in improving drought resistance of plants. The application uses a gene editing technology to knock out a wheat TaSIZ1 gene, and two homozygous knockout strains Tasiz1-6 # and Tasiz1-13 # are screened. Compared with a wild type, the TaSIZ1 gene knockout mutant has a significantly higher fresh weight of an aboveground part and a significantly lower wilting degree under a drought treatment condition; after a rehydration treatment is performed on the plant after the drought stress, the survival rate of the TaSIZ1 gene knockout mutant is significantly improved, the drought resistance of the wheat can be significantly enhanced, and a feasible method for cultivating stress-resistant wheat is provided.

The application discloses a Pseudomonas burcheri ZJL-8 and application thereof, and belongs to the technical field of microorganisms. The application is to solve the problems of environmental pollution caused by chemical fertilizer application and lack of special bacterial agent in the production of blueberry. The technical scheme comprises the following steps: a bacterium ZJL-8 is screened and separated in the rhizosphere soil of blueberry, the bacterium ZJL-8 is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: M 20242867, and the bacterium ZJL-8 is named as Pseudomonas brenneri ZJL-8. The strain has the abilities of nitrogen fixation, inorganic phosphorus dissolution, iron carrier secretion and indole acetic acid (IAA) synthesis, can significantly improve the stem length, root length, fresh weight and dry weight of the blueberry after inoculation, increase the proline content in the leaf, and reduce the malondialdehyde (MDA) content, which indicates that the bacterium has a certain promoting capacity for the growth of the blueberry, and the application provides a technical reference for the large-scale planting and commercial development of the blueberry in the later period.

This invention discloses a disease-resistant and growth-promoting strain of *Streptomyces oryzae* P9-1 and its applications. *Streptomyces oryzae* P9-1 has the accession number CGMCC No. 36797 at the China General Microbiological Culture Collection Center. This strain exhibits typical actinomycete characteristics, excellent salt tolerance, and possesses multiple enzyme activities and potassium-solubilizing activities. Antagonism tests show that this strain has significant inhibitory effects on *Alternaria maculata*, the pathogen of apple tree rot, and *Alternaria spp.*, the pathogen of apple leaf spot. Growth-promoting tests show that this strain can significantly increase the plant height, stem diameter, fresh weight, and various root morphological indicators of maize seedlings. This strain can be used to prepare biocontrol agents for apple diseases and growth-promoting agents for maize, possessing dual application value in both biological control and crop growth promotion.

The application relates to the field of biological technology, in particular to application of cyclic adenosine monophosphate in promoting biomass increase of pasture and root nodule, wherein the cyclic adenosine monophosphate significantly improves the fresh weight biomass of the above-ground part, the root part and the single-plant root system root nodule of Medicago truncatula inoculated with rhizobium, and the difference is significant.

The application discloses a strain of Talaromyces intermedial (D117) inbred in seeds of upland rice, Diaporthe tulliensis The strain has strong abilities of producing indole acetic acid (IAA), dissolving phosphorus and producing iron carrier, can be inbred in rice, can significantly enhance the drought resistance of the rice, and can significantly improve the root length, fresh weight, overground dry weight and underground dry weight of crops, and provides excellent strain resources for development of new microbial fertilizers.

This invention discloses a plant stress resistance gene, MsPAP22, and its applications. When overexpressed in plants (such as alfalfa), the MsPAP22 gene screened in this invention can maintain longer taproots and lateral roots, as well as higher biomass (fresh weight) under salt stress, enhancing the plant's antioxidant defense capabilities and maintaining cell membrane integrity, thereby effectively improving its salt tolerance. MsPAP22 overexpression can effectively alleviate the inhibition of aboveground and root growth caused by low phosphorus stress, increase root length to expand the phosphorus absorption range, and improve phosphorus absorption or utilization efficiency. Under low phosphorus stress, it can maintain higher chlorophyll content, better preserving leaf greenness and photosynthetic system stability.

This invention discloses a strain of *Bacillus halophilus* and its application in promoting sesame growth and improving salt and alkali tolerance, belonging to the field of sesame cultivation technology. This strain of *Bacillus halophilus* is... Halobacillus sp. OCR1-S1, with the preservation number CCTCC NO:M 20252396. Experiments showed that inoculation with the OCR1-S1 strain during the sesame germination stage under salt-alkali stress significantly promoted the growth of fresh weight, root length, and shoot length in sesame buds; inoculation with the OCR1-S1 strain during the sesame seedling stage significantly enhanced the sesame's resistance to salt-alkali stress; simultaneously, treatment of sesame roots with the OCR1-S1 strain under normal soil conditions also promoted sesame plant growth and increased phosphorus content. This invention provides a new microbial resource and a reliable solution for sesame to cope with salt-alkali stress, and is of great significance for utilizing my country's large areas of saline-alkali land to meet the needs of sesame industry expansion.

This invention discloses a semi-dominant gene for controlling maize plant height. GLK53 This invention relates to and its applications, belonging to the field of maize breeding technology. The invention discovers maize... GLK53 Genetic glk53-1 mutants in GLK53 A G / A point mutation occurs 394 bp downstream of the start codon, resulting in a transcript change from wild-type T2 to mutant T1. The T1 transcript has an extra 9 bases at the end of the second exon, causing the insertion of three amino acids VVR into the Myb DNA-binding domain. This mutation has a semi-dominant inheritance characteristic, increasing maize plant height by 25.84%, the total number of leaves by 3.4, and fresh weight by 15.06%; in F1 hybrids, it still significantly increases plant height by 5.71%–10.07% and fresh weight by 13.26%–15.27%. This invention provides a method based on… glk53-1 The breeding methods and molecular markers for mutant maize can be used to rapidly breed tall maize varieties, which has important application value.

A kind of low potassium content of quantitative control of potassium content and its cultivation method and cultivation system, the method is: two sets of high concentration mother liquor of conventional potassium content and no potassium are prepared, after dilution, potassium-containing nutrient solution is continuously supplied in the early stage of plant growth;When the fresh weight of the whole plant reaches the potassium threshold value calculated from the target fresh sample potassium content, conventional lettuce potassium content and aboveground fresh weight, accurately switch to no potassium nutrient solution until harvest;At the same time, replace potassium salt with equimolar sodium salt / calcium salt to maintain conductivity and ion balance during the no potassium stage, fine control with 1.3-1.5 mS / cm EC and 5.5-6.5 pH, ensure root health and yield quality.The system does not require special light source or temperature control facilities, suitable for deep liquid flow, shallow liquid flow and aeroponic multiple soilless platform lettuce production, batch stable to achieve 100±10 mg / 100 g (fresh sample) of low potassium product, with the advantages of simple operation, strong repeatability and high industrialization promotion value.

This invention relates to the field of microbial technology, and more particularly to a strain of *Bacillus subtilis* for kimchi and its applications. The preservation number of *Bacillus subtilis* for kimchi is CGMCC No. 30790. This invention screened a strain of *Bacillus subtilis* for kimchi from saline-alkali soil. This strain possesses the ability to promote plant growth under saline-alkali stress. Applying this strain under saline-alkali stress can effectively increase root length, stem length, fresh weight, and crude protein content of plants, while significantly reducing soil salinity and pH, and increasing available phosphorus content. The *Bacillus subtilis* for kimchi provided by this invention offers a good solution for planting in saline-alkali soil, which has significant application value in the fields of ecological restoration and soil improvement.

This invention discloses the wheat heat-resistant protein TaNAC034, its encoding gene, and its applications, addressing the technical problem of regulating plant heat resistance. Specifically, it discloses the application of the protein, a substance regulating the expression of the gene encoding the protein, or a substance regulating the activity or content of the protein in any of the following: A1) application in regulating plant heat resistance and / or application in preparing products that regulate plant heat resistance; A2) application in regulating plant dry weight under heat stress and / or application in preparing products that regulate plant dry weight under heat stress; A3) application in regulating plant fresh weight under heat stress and / or application in preparing products that regulate plant fresh weight under heat stress; the protein is any of the following: B1) a protein with the amino acid sequence shown in Sequence 2; B2) a protein obtained by substituting and / or deleting and / or adding amino acid residues of the protein in B1) that has more than 80% identity and the same function as the protein shown in B1); B3) a fusion protein obtained by attaching a protein tag to the N-terminus and / or C-terminus of B1) or B2). By regulating the expression of the TaNAC034 gene in plants, heat tolerance can be controlled, which can be used in industrial production.

The application discloses endophytic fungus YT15-1 of Genus Xylaria and application thereof, and belongs to the technical field of microorganisms. Sarocladium strictum The endophytic fungus YT15-1 of Genus Xylaria is isolated from the root system of Sonchus arvensis L., is classified and named as YT15-1, is preserved in the China Center for Type Culture Collection, and has a preservation number of CCTCC NO: M 20252189. The endophytic fungus YT15-1 can colonize the root system of rice by being co-cultured with the rice, and can effectively enhance the salt stress resistance of the rice seedling stage. Under the condition of high salt stress, compared with a control group, the stem and leaf height, root length and stem and leaf fresh weight of the strain treatment group of the endophytic fungus YT15-1 are increased by 75.54%, 214.71% and 52.63% respectively.