349 results about "Fresh weight" patented technology

The invention discloses a PgEGY3 gene for improving cold resistance of pennisetum alopecuroides and increasing fresh weight of leaves and application of the PgEGY3 gene, and relates to the technical field of genetic engineering, and the nucleotide sequence of the PgEGY3 gene of the pennisetum alopecuroides is shown as SEQ ID NO.1. The invention also discloses a protein coded by the Pennisetum alopecuroides PgEGY3 gene. The invention also discloses a recombinant expression vector and a recombinant host cell comprising the Pennisetum alopecuroides PgEGY3 gene. The invention also discloses application of the PgEGY3 gene, a recombinant expression vector and a recombinant host cell in improving cold resistance and biomass of pennisetum alopecuroides. It is verified that the Pennisetum alopecuroides PgEGY3 gene can improve the cold resistance of plants, and the growth state of the plants under cold stress is remarkably improved through overexpression of the PgEGY3 gene.

The invention provides a Kitasatospora xanthophycea JY01 strain and application thereof, the strain is separated from intestinal tracts of spring silkworms, has good disease-resistant and growth-promoting effects, and is specifically expressed in that cellulase, beta-1, 3-glucanase, siderophore and auxin IAA can be secreted; pot experiments show that the biocontrol efficiency of the microbial inoculum to wheat stalk rot is 57.90%, the biocontrol efficiency of the microbial inoculum to corn stalk rot is 46.41%, and the plant height and fresh weight are both significantly increased. The microbial agent is expected to be applied to preparation of biological bacterial fertilizer for effectively preventing and controlling soil-borne fungal diseases.

The invention belongs to the technical field of agricultural microorganisms, and particularly relates to pseudomonas fluorescens as well as a microbial agent and application thereof. The invention discloses a pseudomonas fluorescens TJ194, which is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.33697. The pseudomonas fluorescens TJ194 has the advantages that the pseudomonas fluorescens TJ194 can be used for preparing the pseudomonas fluorescens TJ194, and the pseudomonas fluorescens TJ194 can be used for preparing the pseudomonas fluorescens TJ194; tests prove that the strain has the activity of inhibiting the growth of fusarium pseudograminearum; by applying a fungicide containing the strain, the disease index of the wheat stem rot is remarkably reduced, and meanwhile, the fresh weight of the overground part and the underground part of wheat is increased, so that the pseudomonas fluorescens TJ194 and the fungicide thereof can be applied to prevention and treatment of the plant stem rot and promotion of plant growth.

The invention discloses preparation of a trichoderma composite flora and application of the trichoderma composite flora in prevention and control of crop fusarium wilt and improvement of soil, and relates to the field of agricultural production, and the composite flora is prepared by mixing trichoderma asperellum (5 * 10 < 7 > CFU / L), streptomyces (108 CFU / L), Chinese monospora (108 CFU / L), ensifer adhesion (108 CFU / L), pseudomonas nitroreducens (108 CFU / L) and paenibacillus alvei (108 CFU / L) according to the volume ratio of 6: 1: 1: 1: 1: 1: 1: 1 (V: V). According to the composite flora in the scheme, the composite flora is used for promoting growth of cucumber plants, and compared with a clear water control group (Foc), the fresh weight of cucumbers is increased by 55.51%, the stem diameter is increased by 12.76%, and the plant height is increased by 34.25%. The composite flora can also effectively prevent and treat fusarium wilt of cucumbers, and the prevention and treatment effect reaches 71.43%. Compared with the Trichoderma asperellum FJ035 treated group, the Trichoderma asperellum FJ035 has the advantages that the Trichoderma asperellum FJ035 is better than the Trichoderma asperellum FJ035 which is independently applied in the aspects of promoting the growth of cucumber plants and preventing and treating cucumber fusarium wilt, and compared with the Trichoderma asperellum FJ035 treated group, the control effect is improved by 56.25%. Meanwhile, the composite flora can be used for improving soil and preventing and treating multi-crop fusarium wilt.

The invention discloses a microbial agent and application thereof in preventing and treating rice blast and promoting rice growth, active ingredients of the microbial agent are formed by compounding cold bacillus with the preservation number of CGMCC (China General Microbiological Culture Collection Center) NO: 33492 and bacillus velezensis with the preservation number of CGMCC NO: 33493, and the microbial agent aims at solving the synergistic problem of preventing and treating rice blast and promoting rice growth. According to the complex microbial inoculant, through the synergistic interaction between the two strains, the metabolite of the complex microbial inoculant can completely inhibit the spore germination of magnaporthe oryzae; meanwhile, the fungicide can degrade cellulose, obviously promote the growth of plant height, fresh weight and dry weight of rice, and realize the integration of disease prevention and growth promotion. The invention further discloses a preparation method of the fungicide and an application method of the fungicide for preventing and treating rice blast and promoting growth in a root irrigation mode in the three-leaf stage of rice. The microbial agent disclosed by the invention has dual functions of efficiently preventing diseases and stably promoting growth, and an efficient and safe biological solution is provided for green and sustainable development of agriculture.

The invention relates to the technical field of plant genetic engineering, and discloses application of a soybean low-phosphorus-tolerant gene GmAP2 to improvement of low-phosphorus stress tolerance of plants. Research finds that under the condition of low phosphorus stress, the expression level of the GmAP2 gene in plants is remarkably increased. The GmAP2 gene is subjected to heterologous overexpression in arabidopsis thaliana, so that stress on plants under a low-phosphorus condition can be effectively relieved, the fresh weight and lateral root length of the arabidopsis thaliana can be remarkably increased, and the growth of the arabidopsis thaliana plants is promoted. In addition, under the low-phosphorus condition, overexpression of the GmAP2 gene can improve the phosphorus deficiency symptom of the soybean, improve the absorption of phosphorus nutrients by plants and promote the growth of plant roots and leaves. Therefore, the GmAP2 gene plays a very important role in dealing with the phosphorus starvation stress environment by the plant.

The invention discloses a cold response PaIBH1 protein and application of a coding gene thereof in enhancing cold stress resistance of plants. The cold response PaIBH1 protein is a protein PaIBH1 with an amino acid sequence as shown in SEQ ID NO: 2. Experiments prove that the gene for coding the protein PaIBH1, namely the gene PaIBH1, is overexpressed in wild type arabidopsis thaliana, so that the cold resistance of the arabidopsis thaliana can be improved; the improvement of the cold resistance shows that after cold treatment, the fresh weight of the overground part is increased, the wilting rate is reduced, the leaf membrane damage and electrolyte leakage are reduced, the photosynthetic ability is improved, the membrane lipid peroxidation damage is relieved, the active oxygen scavenging ability is enhanced and / or the active oxygen accumulation in the plant body is reduced. Therefore, the protein PaIBH1 can improve the cold resistance of the plants. The method has a very wide application prospect in the aspect of cold-resistant genetic improvement of crops or pasture.

The invention provides a growth-promoting rhizobacteria for enhancing salt tolerance of corn and application of the growth-promoting rhizobacteria, and belongs to the technical field of agricultural microorganisms. The invention provides a pseudomonas bacterium TJ1-185, and the preservation number of the pseudomonas bacterium TJ1-185 is CDMCC (China Description of Microbiological Culture Collection Center) No.64979. The pseudomonas bacteria TJ1-185 can improve the salt stress resistance of plants and relieve the damage of salt stress to the plants. The result of the embodiment shows that the pseudomonas bacterium TJ1-185 can promote corn to germinate under salt stress, improve the plant height, root length, overground part fresh weight, root fresh weight, overground part dry weight and root dry weight of corn under the salt stress condition, and reduce the malondialdehyde content, thereby promoting the growth of corn under the salt stress condition and improving the salt stress resistance of corn. The damage of salt stress to the corn is relieved.

The invention discloses a method for evaluating and screening salt tolerance of triticale germplasm resources in a germination stage, and belongs to the technical field of evaluation and screening of plant salt tolerance, salt stress concentration screening: triticale seeds are treated by NaCl solutions with different concentrations, indexes such as survival rate, plant height and overground part fresh weight are measured, the NaCl solution with the concentration of 200 mmol. L <-1 > is determined as the salt tolerance evaluation appropriate concentration, and the salt stress concentration is determined as the salt tolerance evaluation appropriate concentration. Salt tolerance evaluation test: carrying out salt stress treatment by adopting a 200mmol. L <-1 > NaCl solution, determining phenotypic indexes such as survival rate, plant height, fresh weight of overground part, root length and chlorophyll content, and physiological and biochemical indexes such as catalase, peroxidase and superoxide dismutase activity and malondialdehyde content, and carrying out principal component analysis, membership function analysis and clustering analysis to obtain a salt tolerance evaluation result. Calculating a salt tolerance comprehensive evaluation value, and screening salt tolerance triticale germplasm resources.

The invention relates to a growth-promoting compound microbial agent, which is prepared from bacillus pseudomycoides, bacillus subtilis and Trichoderma longibrachiatum, and is characterized in that the growth-promoting compound microbial agent is prepared from bacillus pseudomycoides, bacillus subtilis and Trichoderma longibrachiatum, and the growth-promoting compound microbial agent is prepared from bacillus amyloliquefaciens, bacillus subtilis and Trichoderma longibrachiatum. The compound microbial agent (bacillus subtilis, pseudofungal bacillus and trichoderma longibrachiatum) has excellent performance in the aspects of promoting plant growth and improving the soil environment, and the plant height, the fresh weight and the root length of plants are remarkably increased. The application of the complex microbial inoculant effectively relieves the chive continuous cropping obstacle, improves the chive growth potential and soil fertility, and provides a theoretical basis and technical support for sustainable planting of chives in facility agriculture.

The invention discloses three growth-promoting bacteria combined with sinorhizobium freudenreanum and application thereof, and belongs to the technical field of microorganisms. According to the invention, three growth-promoting bacteria of fish pseudomonas ZMC01, pseudomonas puticola ZMC129 and cellulomicrobe vinckii ZMC94 are separated from the root of an oxytropis glabra plant in a saline-alkali beach, and the three growth-promoting bacteria have the ability to grow in a saline-alkali environment. Tests prove that the growth-promoting bacteria ZMC01, ZMC129 and ZMC94 can promote the growth of soybeans in a saline-alkali environment or a non-saline-alkali environment by independently acting or jointly acting with the sinorhizobium freudenreanum, and indexes such as plant height, root length, stem and leaf fresh weight, root fresh weight, root nodule number and root nodule fresh weight of plants are remarkably improved or increased. The growth-promoting bacteria can also improve the nodulation capacity of the sinorhizobium freudenreanum in a saline-alkali environment, the nodulation and nitrogen fixation capacity of the soybeans under saline-alkali stress is expected to be fundamentally improved, and the growth-promoting bacteria have important significance for guaranteeing safe production of the soybeans.

The invention discloses a nano-material complex microbial inoculant and application thereof, and relates to the technical field of microorganisms. The invention discloses application of a nano-material complex microbial inoculant in promoting growth of overground parts of plants, and results show that compared with a treatment group singly inoculated with YH-18, the plant height, leaf area, ground diameter, fresh weight, dry weight and chlorophyll content of tomato seedlings subjected to complex treatment are all remarkably increased. The invention discloses application of a nano-material complex microbial inoculant in promoting plant root growth, and results show that the root length, the root surface area and the root tip number are respectively increased by 35.76%, 13.19% and 57.84% compared with those of single inoculation of YH-18. After compounding and inoculating, the content increase of rapidly available potassium, rapidly available phosphorus and hydrolytic nitrogen in the soil is effectively promoted, the content of organic matters in the soil is remarkably increased, and the nutrient structure of the soil is improved; pseudomonas is significantly enriched through composite inoculation, and the abundance of TRA3-20 is significantly reduced.

The invention relates to a streptomyces sp. Strain EC15 and an application of the streptomyces sp. Strain EC15. The streptomyces strain EC15 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation address is No.3, yard 1, Beichen West Road, Chaoyang District, Beijing, the preservation date is December 25, 2024, and the preservation number is CGMCC NO.33195. The streptomyces strain EC15 has the advantages that the streptomyces strain EC15 can be used for preparing the streptomyces strain EC15; the strain has the functions of dissolving organic phosphorus, dissolving inorganic phosphorus, producing a biological membrane, producing an iron carrier, producing IAA and producing ACC deaminase. The EC15 bacterial liquid is used for soaking seeds under the stress of 200 mmol / L and 250 mmol / L NaCl, so that the germination potential, the germination rate, the germination index, the radicle length, the germ length and the fresh weight of group seedlings of the alfalfa seeds can be obviously improved. After the EC15 strain is inoculated, under the stress of 200 mmol / L NaCl, the plant height, stem diameter, leaf area and overground part fresh and dry weight of alfalfa plants can be obviously improved.

The invention relates to the technical field of microbial fermentation, and discloses an agricultural ecological probiotic complex microbial inoculant, which is prepared from the following components in percentage by mass: 30 to 35 weight percent of beneficial microbial inoculant, 20 to 25 weight percent of mineral source potassium fulvate, 15 to 20 weight percent of slow release agent, 10 to 15 weight percent of nano polypeptide fermentation liquor and the balance of carrier, the processing method comprises the following steps: S1, pretreating the raw materials; s2, preparing nano polypeptide fermentation liquor; s3, complex microbial inoculants are compounded; and S4, drying and forming. According to the invention, the mineral source potassium fulvic acid and the nano-polypeptide fermentation liquor are added, so that effective regulation and control of soil micro-ecology are realized; crop growth is promoted through cooperation of multiple strains, growth hormone is secreted through bacillus subtilis and the like, nitrogen is fixed through bacillus amyloliquefaciens, root development is stimulated in cooperation with mineral source potassium fulvic acid, and the plant height, stem diameter and fresh weight of crops are remarkably increased; the particle size of the polypeptide subjected to high-pressure homogenization treatment is 80-120nm, the absorption efficiency is improved by 60%, and a crop disease-resistant signal channel is activated.

The invention discloses a medicago sativa MsCBR3 gene as well as an encoding protein and application thereof, and belongs to the technical field of plant genetic engineering. According to the invention, a medicago sativa gene MsCBR3 is found, and through construction of a medicago sativa MsCBR3 gene overexpression plant, it is found that compared with a control plant, the number and total length of secondary branches of the plant, the fresh weight of an overground part, and the number of branches and the number of leaves after cutting are significantly increased. Therefore, it is determined that the MsCBR3 gene has a regulation effect on the biomass of medicago sativa. Therefore, the invention provides a basis for cultivating high-yield alfalfa plants and breeding the alfalfa plants, and has great application value.

The invention discloses application of bacillus megaterium capable of synthesizing indole-3-lactic acid to promotion of soybean growth and nodulation, the bacillus megaterium has the characteristic of promoting growth of various plants, and the fresh weight and nodulation of soybeans are remarkably improved; a large amount of indole-3-lactic acid (ILA) is synthesized and secreted, and the fresh weight and nodulation of soybean plants are remarkably improved; the strain has a good application prospect as a biological inoculant for leguminous crops.

The invention provides application of a poplar PtoHsfB1 gene in plant growth, and belongs to the technical field of gene engineering. The function of promoting plant growth of the populus tomentosa gene PtoHsfB1 is found for the first time. Experimental results show that the expression quantity of the PtoHsfB1 gene in the transgenic populus tomentosa is greatly improved compared with that of non-transgenic populus tomentosa, the height, dry weight and fresh weight of a transgenic plant are higher than those of a non-transgenic plant, ABA synthesis and signal transduction can be reduced through overexpression of the gene PtoHsfB1, growth and development of the populus tomentosa are promoted, and the yield of the populus tomentosa is increased. Therefore, a new genetic resource is provided for improvement and molecular breeding of the poplar and other woody plants.

The invention belongs to the technical field of wheat planting, and particularly relates to an exercise planting method for improving the salt tolerance of wheat in the seedling stage, which comprises the following steps: S1, planting first-generation wheat seeds in soil, performing water treatment in the planting period into dry farming and rain culture treatment, harvesting second-generation wheat seeds after the wheat seeds are mature, and setting the first-generation wheat seeds in groups; s2, putting the second-generation wheat seeds into a culture dish, culturing by using a NaCl solution, observing the germination rate and the fresh bud weight or the dry bud weight in the bud stage, and screening the seeds with the emergence rate of more than or equal to 90% and the fresh bud weight or the dry bud weight ranked in the first 30% of the group in the same group. According to the method, the wheat grows in the drought and saline-alkali environment, the salt tolerance of the next generation of wheat is improved through trans-generation stress exercise, meanwhile, the memory ability of seeds is improved through contemporary initiation treatment, and the drought and saline-alkali trans-generation stress exercise is matched with the contemporary initiation treatment to generate a synergistic effect, so that the salt tolerance of the wheat is further improved.

The invention discloses a trans-boundary synthetic flora (SMC) for preventing and treating wheat stem rot as well as preparation and application of the trans-boundary synthetic flora. The synthetic bacterial flora is prepared by compounding Trichoderma harzianum T19 with the preservation number of CCTCC (China Center for Type Culture Collection) No: M 20251896, bacillus subtilis BS-Z15 with the preservation number of CCTCC No: M 20251892, two strains of growth promoting bacillus PM9 and PM16, and two strains of auxiliary bacillus S8 and S32 according to a specific proportion. Compared with an existing single antagonistic bacterial agent, the synthetic flora disclosed by the invention has the advantages that the bacteriostasis rate of the synthetic flora on Fusarium pseudograminearum under a non-sterile soil condition can reach 70% or above, and the disease index is reduced by 70.01%; meanwhile, the plant height of wheat is increased by 28.63%, the root length is increased by 24.53%, the fresh weight is increased by 181.21%, the dry weight is increased by 184.64%, the thousand seed weight is increased by 10.39%, and the rhizosphere microbial community structure and the soil enzyme activity are remarkably improved. The method can be used for green prevention and control and yield increase of basal stem rot of wheat and other gramineous crops.

The invention relates to a plant fresh weight prediction method and system based on monocular depth estimation. The method comprises the following steps that a plant growth image is collected through a digital camera in real time and preprocessed; performing depth prediction on the growth image by using an optimized monocular depth estimation model, and outputting a depth estimation image, the optimized monocular depth estimation model adding a lightweight spatial self-attention module between an encoder and a decoder; segmenting the growth image by using an image segmentation model to obtain a mask, extracting plant areas of the growth image and the depth estimation image by using the mask, and respectively obtaining an RGB segmented image and a depth segmented image; reconstructing a three-dimensional model of the plant by combining spatial information in the RGB segmented image and the depth segmented image; and carrying out feature extraction on the reconstructed three-dimensional model, extracting features related to fresh weight, and carrying out fresh weight prediction on the plant by utilizing the extracted features. Compared with the prior art, the method has the advantages that dynamic accurate prediction can be carried out, and the like.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to the salt tolerance of island cotton and application of the SNP molecular marker. The site of the SNP molecular marker is located at the 45674375th site of a D02 chromosome of gossypium barbadense, and the allele at the 45674375th site is A or G. According to the invention, whole genome association analysis is carried out on the relative plant height and the relative overground part fresh weight in salt stress and salt-free stress environments, the SNP site related to the salt tolerance of the sea island cotton is obtained through screening, and the SNP molecular marker is accurate and efficient in detection and convenient and stable in amplification, can be used for molecular marker-assisted selection, and can be used for detecting the salt tolerance of the sea island cotton. The identification efficiency of different salt-tolerant cotton varieties is improved.

The invention discloses oyster shell-starch sludge carbon quantum dots and application thereof as a pepper growth promoter, and belongs to the technical field of organic fertilizers, soil improvement and waste utilization. Comprising the following steps: crushing oyster shells, mixing the crushed oyster shells with starch sludge, performing hydrothermal reaction, centrifuging, taking supernate, dialyzing, freezing and drying. The promoter can be used for enhancing photosynthesis of pepper leaves, promoting growth and yield increase of pepper in saline-alkali soil, and can be used as a promoter for growth and yield increase of pepper in saline-alkali soil. The plant height, stem diameter, leaf length, leaf width and fresh weight of the pepper treated by the oyster shell starch sludge carbon quantum dots are obviously improved. The maximum quantum yield, the actual photosynthesis efficiency, the absolute electron conductivity and the non-photochemical quenching coefficient of pepper plants can be obviously improved. The plant height, stem diameter, leaf area and single plant yield of pepper plants can be obviously improved when the fertilizer is applied to the field. After the calcium-doped carbon quantum dots are treated, the growth and development of the saline-alkali soil peppers are promoted, the photosynthesis of the saline-alkali soil peppers is promoted, and the yield of the peppers is increased.

The invention discloses a strain NJSH56 for promoting plant growth and application thereof, the strain NJSH56 is identified as bacillus pumilus, the strain NJSH56 is preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation time is November 14, 2024, and the strain preservation number is CGMCC NO.32614. The strain NJSH56 can promote plant growth. The dendrobium officinale endophytic growth-promoting strain NJSH56 for promoting plant growth is obtained through separation and screening, growth of dendrobium officinale tissue culture seedlings can be effectively promoted, and the fresh weight, the rooting number and the leaf number are increased; greenhouse tests show that the number of new buds of dendrobium officinale plants in a strain NJSH56 treatment group reaches 4.67 and is obviously higher than that of a control group; meanwhile, the NJSH56 is used for treating the non-host plant Chinese cabbage, so that the growth of the overground part of the Chinese cabbage can be remarkably promoted, and the biomass accumulation of the Chinese cabbage is improved.

The invention provides a peptide-enriched organic fertilizer as well as a preparation method and application thereof, and belongs to the technical field of organic fertilizer preparation. The preparation method of the peptide-enriched organic fertilizer provided by the invention comprises the following steps: mixing corn soaking water with aspergillus oryzae seed koji, and fermenting for the first time to obtain the peptide-enriched organic fertilizer; the aspergillus oryzae mold starter is obtained by inoculating aspergillus oryzae YY-21 into a culture medium and fermenting 2. According to the preparation method provided by the invention, the peptide-enriched organic fertilizer with high amino acid nitrogen content, high small peptide content, high soluble protein content and high thallus biomass can be prepared, and when the peptide-enriched organic fertilizer is used for planting lettuce, the plant height, the fresh weight of a single plant, the root length, the leaf number and the leaf area of the lettuce can be remarkably increased; the chlorophyll content, the vitamin C content and the total sugar content in the lettuce can be remarkably improved, and the nitrate content of lettuce leaves can be reduced.

The invention relates to the technical field of biology, in particular to a ZmTCP19 gene mutant and application of the ZmTCP19 gene mutant to regulation and control of the nitrogen utilization rate, the growth amount and the plant height of corn. ZmTCP19 gene mutants (a mutant zmtcp19-1 and a mutant zmtcp19-2) are obtained through a molecular genetics means, the functions of the ZmTCP19 gene mutants are deeply analyzed and identified, and results show that under the condition of low nitrogen, the total fresh weight of the zmtcp19-1 is reduced by 30.33% compared with that of a control group, and under the condition of normal nitrogen, the total fresh weight of the zmtcp19-1 is reduced by 25.54% compared with that of the control group; under the condition of low nitrogen, the total fresh weight of zmtcp19-2 is reduced by 25.19% compared with that of a control group, and under the condition of normal nitrogen, the total fresh weight is reduced by 17.30% compared with that of the control group. Meanwhile, the ZmTCP19 gene mutant can reduce the plant height of the corn and improve the lodging resistance of the corn.

The present application relates to the technical field of tomato genetic breeding, and more specifically to molecular markers associated with tomato traits under low-phosphorus stress and their applications. The molecular marker comprises a nucleotide sequence formed by a single nucleotide A>G mutation at position 52526904 of chromosome 11 of the tomato genome. Using this molecular marker, not only can the plant height, fresh weight, and dry weight of tomatoes at the seedling stage under low-phosphorus stress be predicted and identified, but the degree of low-phosphorus tolerance of the plant can also be determined. This molecular marker can assist in cloning genes controlling plant height, fresh weight, and dry weight of tomatoes at the seedling stage under low-phosphorus conditions and in breeding tomatoes for low-phosphorus tolerance, and has important application value.

The invention relates to the technical field of rice planting methods, in particular to a nutrient solution for culturing Guizhou indica rice variety 'Guizhou Red No.1' as well as application and a culture method of the nutrient solution. According to the invention, a nutrient solution specially aiming at 'Guihong No.1' is researched and determined, the nutrient solution is added with micromolecular substances and Br with the final concentration of 0.1-0.6 [mu] M, GABA with the final concentration of 0.3-0.9 mM, melatonin with the final concentration of 0.5-5 [mu] M and salicylic acid with the final concentration of 10-20 [mu] M, and the nutrient solution specially aiming at 'Guihong No.1' can effectively improve the plant height, the biomass (fresh weight), the nitrogen content, the chlorophyll content and the dry weight of a single plant of 'Guihong No.1'. The nutrient solution provided by the invention can solve the technical problem of lack of an effective culture method in the prior art, and can better promote large-scale, intensive and factory planting management and large-area popularization and application of indica rice varieties in Guizhou by utilizing synergistic interaction and advantage complementation among different treatment modes.

The invention discloses a trichoderma complex microbial inoculant capable of promoting wheat growth and preventing and treating wheat stem rot and application of the trichoderma complex microbial inoculant. The trichoderma complex microbial inoculant comprises a trichoderma asperellum strain XX-57 and a trichoderma harzianum strain PY-127, and the preservation number of the trichoderma asperellum strain XX-57 is CGMCC No. 41937; the preservation number of the trichoderma harzianum strain PY-127 is CGMCC (China General Microbiological Culture Collection Center) NO. 41938. The invention further specifically discloses application of the trichoderma complex microbial inoculant in promoting wheat seed germination and wheat growth and preventing and treating wheat basal stem rot, the trichoderma complex microbial inoculant is applied 10-20 days after wheat seedling emergence, and it is found that compared with single trichoderma inoculation, the trichoderma complex microbial inoculant has the promoting effect on wheat plant height, root length, SPAD value, fresh weight and dry weight; the control effect on wheat stem rot is better, and particularly the control effect is more obvious when pathogenic bacteria are inoculated in advance.

The invention relates to the technical field of microorganisms, and discloses a talaromyces funiculi LD-YGH31 and an application of the talaromyces funiculi LD-YGH31 in salt tolerance. The strain is separated from the root system of halophyte, and the preservation number of the strain is CCTCC NO: M 20251815. After the strain is cultured, the strain and plants are co-cultured, and the growth performance and stress resistance of the plants under salt stress can be remarkably improved. Experiments prove that when the strain and tomato seedlings are co-cultured, under the stress condition of 200 mM NaCl salt, the fresh weight and plant height of the tomato seedlings can be remarkably improved, the activity of superoxide dismutase and peroxidase is improved, the content of malondialdehyde and the relative conductivity are reduced, and the K < + > / Na < + > steady state in plant roots and leaves is maintained. The strain is novel, the application method is simple and easy to implement, and the strain can be developed into a microbial fertilizer for saline-alkali soil crop planting and has remarkable agricultural application value.

The application relates to the field of microbial technology and discloses a Pseudomonas asiatica HNHMPs-260 and application thereof. The strain is preserved in the Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC No: 66854. Fermentation supernatant of the strain can prevent and treat root-knot nematode disease of Hami melon caused by Meloidogyne javanica. Experiments show that the fermentation supernatant has strong toxic effects on second instar larvae of root-knot nematodes and can significantly inhibit the hatching of egg masses and egg grains. In addition, the fermentation supernatant has good thermal stability and storage stability, and still maintains more than 80% nematocidal activity after high-temperature treatment at 121 DEG C and long-term storage at 4 DEG C. Meanwhile, the fermentation supernatant of the strain can promote the growth of Hami melon plants, improve physiological indexes such as root length, stem length, fresh weight and dry weight, and has the application potential of being developed into efficient, environment-friendly and multifunctional biological pesticides.