241 results about "Fresh weight" patented technology

The invention discloses a PgEGY3 gene for improving cold resistance of pennisetum alopecuroides and increasing fresh weight of leaves and application of the PgEGY3 gene, and relates to the technical field of genetic engineering, and the nucleotide sequence of the PgEGY3 gene of the pennisetum alopecuroides is shown as SEQ ID NO.1. The invention also discloses a protein coded by the Pennisetum alopecuroides PgEGY3 gene. The invention also discloses a recombinant expression vector and a recombinant host cell comprising the Pennisetum alopecuroides PgEGY3 gene. The invention also discloses application of the PgEGY3 gene, a recombinant expression vector and a recombinant host cell in improving cold resistance and biomass of pennisetum alopecuroides. It is verified that the Pennisetum alopecuroides PgEGY3 gene can improve the cold resistance of plants, and the growth state of the plants under cold stress is remarkably improved through overexpression of the PgEGY3 gene.

The invention belongs to the technical field of agricultural microorganisms, and particularly relates to pseudomonas fluorescens as well as a microbial agent and application thereof. The invention discloses a pseudomonas fluorescens TJ194, which is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.33697. The pseudomonas fluorescens TJ194 has the advantages that the pseudomonas fluorescens TJ194 can be used for preparing the pseudomonas fluorescens TJ194, and the pseudomonas fluorescens TJ194 can be used for preparing the pseudomonas fluorescens TJ194; tests prove that the strain has the activity of inhibiting the growth of fusarium pseudograminearum; by applying a fungicide containing the strain, the disease index of the wheat stem rot is remarkably reduced, and meanwhile, the fresh weight of the overground part and the underground part of wheat is increased, so that the pseudomonas fluorescens TJ194 and the fungicide thereof can be applied to prevention and treatment of the plant stem rot and promotion of plant growth.

The invention discloses a microbial agent and application thereof in preventing and treating rice blast and promoting rice growth, active ingredients of the microbial agent are formed by compounding cold bacillus with the preservation number of CGMCC (China General Microbiological Culture Collection Center) NO: 33492 and bacillus velezensis with the preservation number of CGMCC NO: 33493, and the microbial agent aims at solving the synergistic problem of preventing and treating rice blast and promoting rice growth. According to the complex microbial inoculant, through the synergistic interaction between the two strains, the metabolite of the complex microbial inoculant can completely inhibit the spore germination of magnaporthe oryzae; meanwhile, the fungicide can degrade cellulose, obviously promote the growth of plant height, fresh weight and dry weight of rice, and realize the integration of disease prevention and growth promotion. The invention further discloses a preparation method of the fungicide and an application method of the fungicide for preventing and treating rice blast and promoting growth in a root irrigation mode in the three-leaf stage of rice. The microbial agent disclosed by the invention has dual functions of efficiently preventing diseases and stably promoting growth, and an efficient and safe biological solution is provided for green and sustainable development of agriculture.

The invention discloses a cold response PaIBH1 protein and application of a coding gene thereof in enhancing cold stress resistance of plants. The cold response PaIBH1 protein is a protein PaIBH1 with an amino acid sequence as shown in SEQ ID NO: 2. Experiments prove that the gene for coding the protein PaIBH1, namely the gene PaIBH1, is overexpressed in wild type arabidopsis thaliana, so that the cold resistance of the arabidopsis thaliana can be improved; the improvement of the cold resistance shows that after cold treatment, the fresh weight of the overground part is increased, the wilting rate is reduced, the leaf membrane damage and electrolyte leakage are reduced, the photosynthetic ability is improved, the membrane lipid peroxidation damage is relieved, the active oxygen scavenging ability is enhanced and / or the active oxygen accumulation in the plant body is reduced. Therefore, the protein PaIBH1 can improve the cold resistance of the plants. The method has a very wide application prospect in the aspect of cold-resistant genetic improvement of crops or pasture.

The invention discloses three growth-promoting bacteria combined with sinorhizobium freudenreanum and application thereof, and belongs to the technical field of microorganisms. According to the invention, three growth-promoting bacteria of fish pseudomonas ZMC01, pseudomonas puticola ZMC129 and cellulomicrobe vinckii ZMC94 are separated from the root of an oxytropis glabra plant in a saline-alkali beach, and the three growth-promoting bacteria have the ability to grow in a saline-alkali environment. Tests prove that the growth-promoting bacteria ZMC01, ZMC129 and ZMC94 can promote the growth of soybeans in a saline-alkali environment or a non-saline-alkali environment by independently acting or jointly acting with the sinorhizobium freudenreanum, and indexes such as plant height, root length, stem and leaf fresh weight, root fresh weight, root nodule number and root nodule fresh weight of plants are remarkably improved or increased. The growth-promoting bacteria can also improve the nodulation capacity of the sinorhizobium freudenreanum in a saline-alkali environment, the nodulation and nitrogen fixation capacity of the soybeans under saline-alkali stress is expected to be fundamentally improved, and the growth-promoting bacteria have important significance for guaranteeing safe production of the soybeans.

The invention relates to a streptomyces sp. Strain EC15 and an application of the streptomyces sp. Strain EC15. The streptomyces strain EC15 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation address is No.3, yard 1, Beichen West Road, Chaoyang District, Beijing, the preservation date is December 25, 2024, and the preservation number is CGMCC NO.33195. The streptomyces strain EC15 has the advantages that the streptomyces strain EC15 can be used for preparing the streptomyces strain EC15; the strain has the functions of dissolving organic phosphorus, dissolving inorganic phosphorus, producing a biological membrane, producing an iron carrier, producing IAA and producing ACC deaminase. The EC15 bacterial liquid is used for soaking seeds under the stress of 200 mmol / L and 250 mmol / L NaCl, so that the germination potential, the germination rate, the germination index, the radicle length, the germ length and the fresh weight of group seedlings of the alfalfa seeds can be obviously improved. After the EC15 strain is inoculated, under the stress of 200 mmol / L NaCl, the plant height, stem diameter, leaf area and overground part fresh and dry weight of alfalfa plants can be obviously improved.

The invention discloses a trans-boundary synthetic flora (SMC) for preventing and treating wheat stem rot as well as preparation and application of the trans-boundary synthetic flora. The synthetic bacterial flora is prepared by compounding Trichoderma harzianum T19 with the preservation number of CCTCC (China Center for Type Culture Collection) No: M 20251896, bacillus subtilis BS-Z15 with the preservation number of CCTCC No: M 20251892, two strains of growth promoting bacillus PM9 and PM16, and two strains of auxiliary bacillus S8 and S32 according to a specific proportion. Compared with an existing single antagonistic bacterial agent, the synthetic flora disclosed by the invention has the advantages that the bacteriostasis rate of the synthetic flora on Fusarium pseudograminearum under a non-sterile soil condition can reach 70% or above, and the disease index is reduced by 70.01%; meanwhile, the plant height of wheat is increased by 28.63%, the root length is increased by 24.53%, the fresh weight is increased by 181.21%, the dry weight is increased by 184.64%, the thousand seed weight is increased by 10.39%, and the rhizosphere microbial community structure and the soil enzyme activity are remarkably improved. The method can be used for green prevention and control and yield increase of basal stem rot of wheat and other gramineous crops.

The invention discloses oyster shell-starch sludge carbon quantum dots and application thereof as a pepper growth promoter, and belongs to the technical field of organic fertilizers, soil improvement and waste utilization. Comprising the following steps: crushing oyster shells, mixing the crushed oyster shells with starch sludge, performing hydrothermal reaction, centrifuging, taking supernate, dialyzing, freezing and drying. The promoter can be used for enhancing photosynthesis of pepper leaves, promoting growth and yield increase of pepper in saline-alkali soil, and can be used as a promoter for growth and yield increase of pepper in saline-alkali soil. The plant height, stem diameter, leaf length, leaf width and fresh weight of the pepper treated by the oyster shell starch sludge carbon quantum dots are obviously improved. The maximum quantum yield, the actual photosynthesis efficiency, the absolute electron conductivity and the non-photochemical quenching coefficient of pepper plants can be obviously improved. The plant height, stem diameter, leaf area and single plant yield of pepper plants can be obviously improved when the fertilizer is applied to the field. After the calcium-doped carbon quantum dots are treated, the growth and development of the saline-alkali soil peppers are promoted, the photosynthesis of the saline-alkali soil peppers is promoted, and the yield of the peppers is increased.

The invention discloses a strain NJSH56 for promoting plant growth and application thereof, the strain NJSH56 is identified as bacillus pumilus, the strain NJSH56 is preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation time is November 14, 2024, and the strain preservation number is CGMCC NO.32614. The strain NJSH56 can promote plant growth. The dendrobium officinale endophytic growth-promoting strain NJSH56 for promoting plant growth is obtained through separation and screening, growth of dendrobium officinale tissue culture seedlings can be effectively promoted, and the fresh weight, the rooting number and the leaf number are increased; greenhouse tests show that the number of new buds of dendrobium officinale plants in a strain NJSH56 treatment group reaches 4.67 and is obviously higher than that of a control group; meanwhile, the NJSH56 is used for treating the non-host plant Chinese cabbage, so that the growth of the overground part of the Chinese cabbage can be remarkably promoted, and the biomass accumulation of the Chinese cabbage is improved.

The invention provides a peptide-enriched organic fertilizer as well as a preparation method and application thereof, and belongs to the technical field of organic fertilizer preparation. The preparation method of the peptide-enriched organic fertilizer provided by the invention comprises the following steps: mixing corn soaking water with aspergillus oryzae seed koji, and fermenting for the first time to obtain the peptide-enriched organic fertilizer; the aspergillus oryzae mold starter is obtained by inoculating aspergillus oryzae YY-21 into a culture medium and fermenting 2. According to the preparation method provided by the invention, the peptide-enriched organic fertilizer with high amino acid nitrogen content, high small peptide content, high soluble protein content and high thallus biomass can be prepared, and when the peptide-enriched organic fertilizer is used for planting lettuce, the plant height, the fresh weight of a single plant, the root length, the leaf number and the leaf area of the lettuce can be remarkably increased; the chlorophyll content, the vitamin C content and the total sugar content in the lettuce can be remarkably improved, and the nitrate content of lettuce leaves can be reduced.

The invention relates to the technical field of biology, in particular to a ZmTCP19 gene mutant and application of the ZmTCP19 gene mutant to regulation and control of the nitrogen utilization rate, the growth amount and the plant height of corn. ZmTCP19 gene mutants (a mutant zmtcp19-1 and a mutant zmtcp19-2) are obtained through a molecular genetics means, the functions of the ZmTCP19 gene mutants are deeply analyzed and identified, and results show that under the condition of low nitrogen, the total fresh weight of the zmtcp19-1 is reduced by 30.33% compared with that of a control group, and under the condition of normal nitrogen, the total fresh weight of the zmtcp19-1 is reduced by 25.54% compared with that of the control group; under the condition of low nitrogen, the total fresh weight of zmtcp19-2 is reduced by 25.19% compared with that of a control group, and under the condition of normal nitrogen, the total fresh weight is reduced by 17.30% compared with that of the control group. Meanwhile, the ZmTCP19 gene mutant can reduce the plant height of the corn and improve the lodging resistance of the corn.

The invention relates to the technical field of rice planting methods, in particular to a nutrient solution for culturing Guizhou indica rice variety 'Guizhou Red No.1' as well as application and a culture method of the nutrient solution. According to the invention, a nutrient solution specially aiming at 'Guihong No.1' is researched and determined, the nutrient solution is added with micromolecular substances and Br with the final concentration of 0.1-0.6 [mu] M, GABA with the final concentration of 0.3-0.9 mM, melatonin with the final concentration of 0.5-5 [mu] M and salicylic acid with the final concentration of 10-20 [mu] M, and the nutrient solution specially aiming at 'Guihong No.1' can effectively improve the plant height, the biomass (fresh weight), the nitrogen content, the chlorophyll content and the dry weight of a single plant of 'Guihong No.1'. The nutrient solution provided by the invention can solve the technical problem of lack of an effective culture method in the prior art, and can better promote large-scale, intensive and factory planting management and large-area popularization and application of indica rice varieties in Guizhou by utilizing synergistic interaction and advantage complementation among different treatment modes.

The invention relates to the technical field of microorganisms, and discloses a talaromyces funiculi LD-YGH31 and an application of the talaromyces funiculi LD-YGH31 in salt tolerance. The strain is separated from the root system of halophyte, and the preservation number of the strain is CCTCC NO: M 20251815. After the strain is cultured, the strain and plants are co-cultured, and the growth performance and stress resistance of the plants under salt stress can be remarkably improved. Experiments prove that when the strain and tomato seedlings are co-cultured, under the stress condition of 200 mM NaCl salt, the fresh weight and plant height of the tomato seedlings can be remarkably improved, the activity of superoxide dismutase and peroxidase is improved, the content of malondialdehyde and the relative conductivity are reduced, and the K < + > / Na < + > steady state in plant roots and leaves is maintained. The strain is novel, the application method is simple and easy to implement, and the strain can be developed into a microbial fertilizer for saline-alkali soil crop planting and has remarkable agricultural application value.

The application relates to the field of microbial technology and discloses a Pseudomonas asiatica HNHMPs-260 and application thereof. The strain is preserved in the Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC No: 66854. Fermentation supernatant of the strain can prevent and treat root-knot nematode disease of Hami melon caused by Meloidogyne javanica. Experiments show that the fermentation supernatant has strong toxic effects on second instar larvae of root-knot nematodes and can significantly inhibit the hatching of egg masses and egg grains. In addition, the fermentation supernatant has good thermal stability and storage stability, and still maintains more than 80% nematocidal activity after high-temperature treatment at 121 DEG C and long-term storage at 4 DEG C. Meanwhile, the fermentation supernatant of the strain can promote the growth of Hami melon plants, improve physiological indexes such as root length, stem length, fresh weight and dry weight, and has the application potential of being developed into efficient, environment-friendly and multifunctional biological pesticides.

This invention provides an application and method for using *Gastrodia galactosum* NBG001 to promote the germination of *Isatis indigotica* seeds or the growth of seedlings, belonging to the field of plant growth promotion technology. The *Gastrodia galactosum* NBG001 was deposited at the China Center for Type Culture Collection on July 7, 2025, with accession number CCTCC NO: M 20251547. After treating *Isatis indigotica* seeds with *Gastrodia galactosum* NBG001, the germination rate of *Isatis indigotica* increased to 40.6%, which is 1.6 times that of the control group. After transplanting, the fresh weight, number of leaves, and number of roots of *Isatis indigotica* seedlings treated with *Gastrodia galactosum* NBG001 were significantly higher than those of the control group, increasing by 1.6, 1.5, and 1.2 times, respectively.

This invention provides a two-stage cultivation method for Ophiopogon japonicus, belonging to the field of Ophiopogon japonicus planting technology. The two-stage cultivation method includes the following steps: dividing the planting field into a paddy field and an Ophiopogon japonicus seedling field, and planting rice and Ophiopogon japonicus seedlings together in the paddy field and the Ophiopogon japonicus seedling field respectively; after the rice harvest, planting the Ophiopogon japonicus seedlings in the planting field. This invention enables crop rotation of Ophiopogon japonicus and rice within the same planting field within one year, ensuring Ophiopogon japonicus production while increasing rice production for one season, solving the problem of competition for land between medicinal and grain crops, thus protecting both medicinal and grain crops. Compared with traditional cultivation methods, the cultivation method of this invention significantly improves indicators such as leaf length, root number, number of fruit roots per plant, fresh weight of stems and leaves, fresh weight of roots, fresh weight of Ophiopogon japonicus per plant, and yield per acre. The two-stage cultivation method of this invention results in high Ophiopogon japonicus yield and low cost, while also increasing rice production. Therefore, the net benefit of the two-stage cultivation method of this invention is far higher than that of traditional cultivation methods.

The invention belongs to the technical field of gene engineering, and particularly relates to application of PaVAMP711-a protein in regulating and controlling potassium stress resistance of plants. The PaVAMP711-a protein disclosed by the invention is a vesicle transport related protein and belongs to a class of SNARE proteins, and the potassium stress resistance of plants can be improved by knocking out the PaVAMP711-a gene. Through a gene editing technology, the PaVAMP711-a gene in the poplar is knocked out, two obtained transgenic lines have high potassium stress resistance phenotypes, and the fresh weight and the plant height of the transgenic lines are remarkably increased compared with those of wild types. Theoretical basis and technical support are provided for reasonable fertilization, soil improvement and cultivation of high-potassium-stress-resistant forest tree varieties in production, so that the growth performance and yield of crops in an adverse soil environment are improved, and sustainable development of forestry is guaranteed.

The invention discloses a plant growth-promoting bacterium and application thereof, relates to the field of microorganisms, and provides a plant growth-promoting bacterium which can generate a growth-promoting effect on corn plants and further improve the growth condition of the corn plants. The growth-promoting bacterium is pumilus minutus E2 and is preserved in the China Center for Type Culture Collection, the preservation address is Wuhan University in Wuhan, the preservation date is September 19, 2025, and the preservation number is CCTCC NO: M 20252071. By applying the strain to a corn culture medium, the bifurcation number, the root tip number, the root length, the dry weight, the fresh weight and the plant height of corn can be increased, the growth promoting effect of the strain on corn plants is proved, and a basic support is provided for further application to production.

The invention provides a penicillium oxalicum strain K3R2 and a preparation method and application of a biological agent of the penicillium oxalicum strain K3R2. The biocontrol bacterium Penicillium oxalicum strain K3R2 screened by the invention is preserved in the China General Microbiological Culture Collection Center on September 25, 2025, the preservation number is CGMCC NO.42219, and the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No. 3, No.1 yard, Beichen West Road, Chaoyang District, Beijing; the ITS sequence of the Penicillium oxalicum strain K3R2 is as shown in SEQ ID NO: 1, and the CaM sequence is as shown in SEQ ID NO: 2. The penicillium oxalicum strain K3R2 provided by the invention can inhibit hypha growth and spore germination of beet neocam spores, the hypha inhibition rate is 83.60%, the spore inhibition rate is 65.31%, and the penicillium oxalicum strain K3R2 has the effect of preventing and treating quinoa stem blight. Meanwhile, the penicillium oxalicum strain K3R2 has the capabilities of salt resistance, alkali resistance, phosphate solubilization, potassium solubilization, cellulose solubilization and nitrogen fixation; the strain K3R2 remarkably promotes the growth of quinoa plants, so that the chlorophyll content is increased by 20.22%, and the accumulation of nutrient substances is accelerated; the fresh weight, the dry weight and the stem diameter of quinoa plants are increased, and the lodging resistance is enhanced.

The application provides a growth promoting microbial preparation for relieving saline-alkali stress and application thereof, and belongs to the technical field of agricultural microorganisms. The microbial preparation provided by the application can significantly relieve the adverse effect of saline-alkali stress on the growth and development of corn, and can stably improve the growth indexes of corn such as plant height, stem diameter, fresh weight, dry weight, leaf area index and chlorophyll content in different saline-alkali degree plots, and continuously promotes the growth in the whole growth period of corn, especially promotes the grain fresh weight in the late growth period, which is beneficial to yield formation. Meanwhile, the different strains in the compound microbial preparation have good interaction ability, can play the synergistic effect of multiple bacteria and functional complementary effect, and further improve the adaptability and growth promoting effect of the microbial preparation in the complex soil environment. The application has the advantages of environmental friendliness, low cost, difficulty in producing drug resistance and the like, and has a good application prospect in the fields of agricultural yield increase in saline-alkali land and development of microbial fertilizers.

The application belongs to the technical field of vegetable cultivation, and particularly relates to a seed priming method for improving radish seedling emergence rate. The radish seeds are primed by using water extract of broccoli seeds, and are dried through variable-temperature drying, so that seed germination in the priming process is avoided, and indexes such as seedling emergence rate, seedling emergence time and seedling fresh weight are significantly improved, and the improvement effect is obviously better than that of water priming and polyethylene glycol priming. The problem of low radish seedling emergence rate is effectively solved.

The invention belongs to the technical field of agricultural biology, and particularly relates to a method for promoting germination of celery seeds based on an acidic electrolyzed water initiation technology, which comprises the following steps: soaking the celery seeds in an acidic electrolyzed water solution for acidic electrolyzed water initiation treatment, and culturing in an artificial plant incubator to promote seed germination after treatment; the germination time of the celery seeds is shortened to 3 days, the germination rate is remarkably increased, and the germination rate is increased by 6% compared with that of a control group and is increased by 29% compared with that of a control group; the fresh weight is increased by about 0.27 g compared with that of a control group, and is increased by about 0.11 g compared with that of water initiation treatment; the germination index is 2.4 times that of a control group and 1.4 times that of water initiation treatment; the vitality index is 6.6 times that of a control group and 1.8 times that of water initiation treatment; the method provided by the invention can improve the germination characteristics of the celery seeds, and has important theoretical value and practical significance for developing industrialized seedling production of celery and promoting quality improvement and efficiency improvement of the celery industry in China.

The invention discloses application of gold nanoclusters in improvement of salt tolerance of cotton. According to the application, a solution containing the L-gold nanoclusters and / or the D-gold nanoclusters is applied to cotton roots or leaves. The concentration of the L-gold nanocluster in the solution is 0.001 to 0.01 mM; or in the solution, the concentration of the D-gold nanocluster is 0.003 to 0.008 mM. The gold nanocluster can well improve the fresh weight of the cotton under salt stress, can improve the chlorophyll content and photosynthesis of the cotton under salt stress, and can improve the oxidation resistance of the cotton under salt stress.

The application discloses a kind of stem and leaf intelligent separation and high-precision measuring device and method, and it is related to plant phenotype analysis technical field.The device includes clamping module, weighing module, visual identification module, intelligent separation module, stem processing module, leaf processing module, chopping module, drying and weighing module and central control system.After clamping module fixes plant sample, weighing module obtains initial fresh weight;Visual identification module constructs stem and leaf point cloud model by three-dimensional scanning;Intelligent separation module plans cutting path based on model and executes separation;Stem processing module measures stem diameter and fresh stem weight;Leaf processing module flattens leaf and measures leaf area;After stem and leaf are chopped, send into drying and weighing module, and determine dry weight stable point by automatic weighing.The application integrates separation, measurement, drying function in one, realizes full-process automation, solves the problem of low efficiency, sample easy to damage, poor data consistency of traditional method, significantly improves the precision and efficiency of plant phenotype parameter acquisition.

The invention discloses a serratia pumila strain for preventing and treating soybean root rot and application of the serratia pumila strain. The serratia pumila strain G25-16 is separated from rhizosphere soil of soybeans and wheat, and the microbial preservation number of the serratia pumila strain G25-16 is CGMCC (China General Microbiological Culture Collection Center) No.34772; a plate confrontation experiment result shows that the strain has an obvious antagonistic effect on fusarium solani and fusarium malodosa; a control effect experiment result shows that the strain has a remarkable control effect on fusarium solani and fusarium malodosa; potted plant growth promotion experiments find that after soybeans are treated by the separated serratia pumila strain, the fresh weight and plant height of the soybeans are obviously improved compared with those of a contrast. The serratia pumila strain G25-16 separated by the invention has application prospects in the aspects of promoting soybean growth, preventing and treating soybean diseases and the like.

The application discloses a seedling stage resistance identification method for salvia miltiorrhiza root knot nematode disease and application thereof. After seedling of salvia miltiorrhiza seeds to the 5-6 leaf stage, 2mL of a 2-stage young nematode (J2) suspension with a concentration of 200 pieces / mL is inoculated, and the root knot condition of salvia miltiorrhiza and the influence of different infection durations on the growth condition of salvia miltiorrhiza are investigated after inoculation. The number of root knots of the to-be-tested variety is investigated on the 9th day after inoculation of the nematodes, if the number of root knots is less than 15, the to-be-tested variety enters the next stage of identification, that is, comprehensive trait investigation is carried out on the to-be-tested variety on the 21st day after inoculation of the nematodes, if there is no significant difference between the root fresh weight, the height of the aboveground part and the leaf fresh weight of the to-be-tested variety and those of the non-inoculation group, the to-be-tested variety is preliminarily determined as a resistant variety. The application provides a rapid, simple and efficient indoor identification method for seedling stage resistance identification of salvia miltiorrhiza root knot nematode disease.

The present application relates to the technical field of plant genetic engineering, and particularly relates to an application of Medicago sativa MsPL19 protein and gene in regulating plant height, fresh weight or biological yield of Medicago sativa. The present application provides the MsPL19 protein, gene and application thereof, which can significantly improve the plant height, biological yield and fresh weight of Medicago sativa, and further improve the quality of Medicago sativa. Meanwhile, it is found that the MsPL19 protein and gene can respond to low temperature, drought, salt, hormone and other pathways, the proteins interacting with the MsPL19 are obtained through immunoprecipitation-mass spectrometry analysis, the corresponding molecular module can be enriched, the molecular mechanism of Medicago sativa MsPL19 responding to low temperature environment is analyzed, and a new cold-resistant variety can be selected in an alpine region, thereby providing a new breeding direction for improving the yield of Medicago sativa.

According to the folium artemisiae argyi extract, the preparation method thereof and the application of the folium artemisiae argyi extract in growth promotion, the folium artemisiae argyi extract required by a plant biostimulant comes from folium artemisiae argyi, the folium artemisiae argyi extract has the advantages of being rich in source, easy to extract and the like, green and sustainable agricultural development is facilitated, and a new scheme is provided for yield increase of crops. According to the extract, leaves and stems of traditional Chinese medicine folium artemisiae argyi serve as raw materials and are subjected to two times of ultrasonic extraction (absolute ethyl alcohol), filtrate is combined, then a crude extract is prepared, a purified component is a 60% ethanol component obtained by separating the crude extract through macroporous resin, the purified component mainly comprises a flavonoid component, and the content of eupatilin is the highest. The plant height, leaf length and fresh weight of rice, pakchoi, cotton and soybean are all improved after the fertilizer is applied according to the application mode mentioned in the invention.

The invention relates to the technical field of weed suppression active substances, in particular to application of 2-piperidinecarboxylic acid in inhibition of barnyard grass seed germination and growth. According to the method, the influence of the 2-piperidinecarboxylic acid on the germination of the barnyard grass seeds is investigated by taking the germination rate of the barnyard grass seeds as an index, and the influence of the 2-piperidinecarboxylic acid on the growth of the barnyard grass is investigated by taking the root length, the seedling length / plant height and the fresh plant weight of the barnyard grass seedlings as indexes. The inhibition effect is enhanced along with the increase of the concentration of the 2-piperidinecarboxylic acid, and under the inhibition effect concentration, the 2-piperidinecarboxylic acid is safe to the growth of transplanted rice seedlings and does not generate obvious phytotoxicity. Therefore, the 2-piperidinecarboxylic acid can be effectively applied to inhibition of germination and growth of the barnyard grass seeds, and can also be applied to preparation of products for inhibiting germination and growth of the barnyard grass seeds.

The application discloses a method for controlling low-temperature stress of tomatoes based on nano copper sulfide, and belongs to the technical field of novel cold resistance. The application prepares copper sulfide nanoparticles, and uses the copper sulfide nanoparticles to promote the low-temperature stress resistance of tomatoes. The application controls the low-temperature stress of tomatoes by foliar application of CuS NPs, so that the plant height of the tomatoes under low-temperature stress is increased by 14.96%, and the fresh weight of the aboveground part and the fresh weight of the underground part are respectively increased by 32.21% and 64.37%. The prepared copper sulfide nanoparticles are superior to conventional copper-based materials, sulfur-based materials, traditional cold resistance agents and other sulfur-based nanoparticles in effect.