738 results about "Fresh weight" patented technology

The invention relates to a determining method for indicating salt tolerance of different varieties of barleys at seedling stages and belongs to the field of agriculture. The determining method includes adopting a water culturing manner to simulate salt environment artificially, investigating salt damage degree of samples to be tested under the condition of salt stress compared with the blank control condition by measuring seedling height, root length and fresh weight of each seedling of different treatment, and finally determining salt tolerance of the variety. The determining method can be widely applied to screening and evaluation of plant stress resistance genetic resources, particularly used for screening of barley salt-tolerance variety resources, salt-tolerance QTL (quantitative trait locus) genetic analysis, salt-tolerance gene positioning and seed selection of salt-tolerance barley varieties. The determining method has the advantages of simplicity, easiness in mastering and reliable results, the whole salt-tolerance determining process is under control and is slightly affected by the environment, requirements for equipment are low so that only one common illumination culturing box is required.

The invention discloses a rapid propagation method of culturing medical anoectochilus roxburghii tissue through which seedling is formed in only one step. The invention mainly consists of the flowing steps: firstly, culture medium is prepared by three steps of preparation, prepackaging and sterilization; secondly, explant is treated and inoculated, which step has two conditions: when a natural growth plant is used as an explant, the step consists of three steps of cleaning, sterilizing and inoculation, and when a tissue culture sterilized seedling is used as an explant, the sterilized inoculation is directly carried out; thirdly, cultivation; and fourthly, transplantation. The invention uses the middle stem of an anoectochilus roxburghii plant, which is produced by subculture, as an explant. A stem section which contains a stem node is inoculated on culture medium under sterile condition. The results show that after the optimum culture medium is cultured for four months, the average seedling number of each explant is 4.8; the average net increased number of the leaves of each plant is 3.6; the average increased root number is 2.5 and the average net increased fresh weight is 525.6mg. The growing condition of the tissue culture seedling is quite good after being transplanted to substrate. The survival rate of plants achieves to above 95 percent after three months. The invention can greatly shorten the tissue culture seedling time of anoectochilus roxburghii, significantly improve the culture efficiency and reduce the seedling cost. The invention can be used as an important method of producing anoectochilus roxburghii seedlings.

The invention discloses a bacillus atrophaeus strain DPPG-28 and an application in crop disease control, and belongs to the field of agricultural biotechnology. The strain is separated from soil of drip irrigation fields with damping off in tomato processing production areas, and has an accession number of CGMCC No. 9394. The strain can be used for biological control of crop pathogens such as rhizoctonia solani, pythium aphanidermatum, alternaria solani, phytophthora capsici, tomato scab fungi, and the like; the control effects on tomato damping off, capsicum early blight, and cotton damping off reach respectively 74.17%, 66.83%, and 70.12%; the strain can significantly improve the crop germination rate, plant height, fresh weight, and dry weight at the seedling stage. The strain of the invention has high-efficient wide-spectrum germicidal activity, and has wide application prospects in biocontrol agents.

The invention discloses a film mulching and ridging salt-tolerance planting method for sweet sorghum in coastal beach saline-alkali soil, and belongs to the technical field of crop growing. According to the method, base fertilizer is sufficiently applied in the saline-alkali soil in a coastal beach area, wherein the salt content of the saline-alkali soil is lower than 0.5%; after the soil is finely prepared, ditching is carried out to make beds according to the standard that the width of each bed face is 3-4m; the sweet sorghum is planted in large lines and small lines in suitable density according to the salt content of the soil, wherein the large line spacing is 80cm, and the small line spacing is 40cm; sweet sorghum species of which the growth period ranges from 110 days to 120 days are selected and used to be seeded in the last ten-day period of May and the first ten-day period of June; if soil moisture content is low, irrigation can be carried out to improve the soil moisture content, and seeding is carried out after the soil moisture content reaches a suitable value; after seeding and earthing, the small lines are covered by mulching film, small high ridges with the height of about 5cm and the width of about 10cm are heaped at the film edges, and seedlings are led out of the film timely after emerging. The high-yield culture techniques such as final singling and seedling keeping management, base fertilizer application, jointing fertilizer dressing and disease pest and weed integrated control are adopted after seedling emerging for field management, and the high yield of the sweet sorghum with the stem and leave fresh weight reaching 4-5ton per mu can be achieved.

The invention provides a water culture technique for anoectochilus formosanus and relates to a pollution-free water (fog) culture production technique for Chinese medicament anoectochilus formosanus. The water culture technique adopts plastic disks and water grooves as culture containers and comprises the following steps of: transplanting the seedlings which are prepared by tissue culture and subjected to cleaning and sterilizing into the plastic disks; wherein the plastic disks are placed in the water grooves with nutrient solution; and performing culture management by fertilizing, changing the nutrient solution, atomizing, lighting, controlling humidity, controlling temperature and changing air. The water culture technique has the advantages that: the survival rate of transplanting the noectochilus formosanus seedlings prepared by tissue culture can be improved to over 98 percent; the fresh weight of the finished products in each square meter area is over 800 g; the production process is simple; the yield is high; the occupied area is small; and the water culture technique is suitable for culture by intensive and pollution-free facilities or scattered culture by farmers.

The invention relates to a herbicide composition for preventing and removing farmland weed and an application, in particular to a herbicide used for preventing gramineous weed and broadleaf weed in cornfields, which is a ternary herbicide mixed agent taking nicosulfuron, artrex and sodium sulcotrione as active components. In the composition, the weight ratio of the nicosulfuron to the artrex to the sodium sulcotrione is 1-50:1-80:1-50, preferably 1-10:1-50:1-20. The invention has reasonable components, better preventing effect in comparison with the activity of each component when being applied independently and synergistic action, expands the preventing spectra of the weed, reduces the application frequency, lowers the preventing cost, delays the generation of the resistibility of the weed, has good safety to crops and conforms to the safety requirement of pesticide preparations. The invention has the fresh-weight average preventing effect of 86.19-96.37% to the gramineous weed and the broadleaf weed in the cornfields.

The invention aims at providing a method for efficiently and quickly producing a bletilla striata seedling. The method comprises (1) a liquid culture stage and (2) a solid medium culture stage, wherein (1) the liquid culture stage comprises the particular steps that a bletilla striata capsule is disinfected via sodium hypochlorite; the capsule is cut; a seed is sown in a liquid medium for culture; the seed is developed into a protocorm; (2) the solid medium culture stage comprises the particular steps that the protocorm is induced to be differentiated into a root-free seedling, and then transferred to a rooting culture medium for rooting culture, and a cultivation substrate transplanted into a greenhouse is domesticated before field transplanting. The method realizes efficient and quick production of the bletilla striata seedling; compared with whole-course solid culture, the method overcomes the defect that the seed germination rate is low when an early seed is cultured on a solid medium; the seed germination rate is increased significantly and reaches 99%; the fresh weight and the dry weight of the protocorm are increased greatly; and the whole production cycle is shortened significantly, and can be shortened to about 110 days.

The invention discloses separation of arbuscular mycorrhizal fungi as well as preparation and application of arbuscular mycorrhizal fungi fungicide, and belongs to the technical field of biology. The arbuscular mycorrhizal fungi is arbuscular mycorrhizal fungi rhizophagus irregularis which is called arbuscular mycorrhizal fungi rhizophagus irregularis CD-1 and is separated from soils; a specific sequence of verifying ribosome 28S rDNA is shown in SEQ ID NO.1; by utilizing the fungicide prepared by the fungi, the height of strains, the number of petioles, the overground fresh weight, the overground dry weight, the root fresh weight and the root dry weight of tomatoes can be increased, and the mycorrhizal infection rate delta gt can be obviously prompted; 80 percent of corns are high in selfing line strains and heavy in overground fresh weight and overground dry weight; the resistance to banded sclerotial blight by the corns is improved, and the arbuscular mycorrhizal fungi has an extremely-high genetic use value.

The invention provides a method of detecting the growth promoting effect of plant rhizosphere growth promoting bacteria, which comprises the following steps:(1) solution of plant rhizosphere growth promoting bacteria is prepared and seeds after germination acceleration are soaked in the above solution for 2-4 hours;(2) double filter papers are soaked in the above solution and the seeds are placed at the center of the double filter papers; the double filter papers are then wrapped into a cylinder and placed in a test tube containing nutrient solution; (3) stem length, root length, dry and fresh weights of the seeds are measured after the double filter papers is placed in a climate incubator for 3-4 days. The above method is applicable to the rapid screening of plant rhizosphere growth promoting bacteria and rapid measurement of promoting effect of plant growth promoting materials. The screening time of plant rhizosphere growth promoting bacteria is reduced from one month when the seeds are cultivated in a greenhouse to one week in the present invention. The above method is of great importance to the research and industry development of microorganism fertilizers.

The invention provides a maize salt tolerance identification method. According to the method, the salt tolerance of materials is evaluated by comparing the fresh weights of the overground parts, the dry weights of the overground parts and the K+/Na+ ion content ratios of different materials which are subjected to salt stress. The experiment period is short, one month is enough from the start of treatment to the end of investigation, and the problem of long period of field identification is avoided. Operation is easy, identification indices only include material weights and ion contents, and the method is suitable for large-scale maize material identification. Furthermore, the fresh weights of the overground parts, the dry weights of the overground parts and the K+/Na+ ion content ratios are adopted as the identification indices to conduct mutual verification with the overground part dry weight and fresh weight indices, and manual operating errors are avoided. The method has the advantages of being accurate and reliable.

The method discloses a method for promoting hardening-off and rooting of dendrobium tissue culturing seedlings. The method comprises the following steps: 1, transferring the dendrobium tissue culturing seedlings to a specific hardening-off and rooting culture medium for illumination culture, and performing hardening-off and root induction; 2, hardening the rooted tissue culturing seedlings and then transplanting; and 3, transplanting regenerated plants of the rooted dendrobium tissue culturing seedlings to a culture medium mixing bark with vermiculite. The method is simple to operate and is feasible and quick in hardening-off, each plant is 3-4 cm higher than that of a control group, the diameter of the stem is 2-3mm larger than that of the control group, the fresh weight is 3 times larger than that of the control group, early rooting can be realized, roots can be formed in 4-5 days, the rooting rate is as high as 100% within 30 days, each plant can take 7-8 roots, and the transplanting survival rate is as high as 95%; the time for root induction can be effectively shortened, the root development is good, the plants are robust, the transplanting survival rate is high, and important reference value is achieved for culture of other orchidaceae tissue culturing seedlings.

The invention relates to a method for improving growth performance of lawn plants by adopting rare earth lanthanum. The method comprises the following steps of: soaking seeds of lawn plants with lanthanum nitrate water solution at a concentration of 50mg.L<-1>-300mg.L<-1> for 18-24 hours; then sowing the seeds in a culture dish with filter paper spread, and putting the culture dish with the sowed seeds in the sun to germinate the seeds; beginning recording the germination number 2-3 days after sowing the seeds; and after grass seed growth ends, cutting off the plants at ground level with scissors, measuring the fresh weight of the single plant and the total fresh weight, drying the samples in a drying box at 80 DEG C for 8 hours, and measuring each index. Adopted to soak the seeds, the rare earth lanthanum has an influence on germination and initial growth of Lolium perenne L. and Festuca arundinacea.L, has an effect on promoting germination of the two grass seeds, and has an obvious effect on promoting growth of the underground root system parts. Therefore, the research results show that the rooting degrees and green degrees of the grass seeds on the lawns are regulated to achieve the best effects by soaking the seeds with the rare earth element.

The invention relates to a method for producing dendrobium officinale tissue culture mycorrhizal seedlings, which comprises the following steps of: (1) culturing mycorrhizal fungi by using a solid or liquid culture medium, wherein the mycorrhizal fungi are mycorrhizal fungi LP1, namely Chaetomium sp.; (2) culturing mycorrhizal bacteria, wherein the mycorrhizal bacteria DP6 are Azotobacter sp.; (3) culturing the dendrobium officinale tissue culture seedlings; and (4) carrying out water culturing and training on the tissue culture seedlings. With the method for producing the dendrobium officinale tissue culture mycorrhizal seedlings, provided by the invention, not only the growth of the dendrobium officinale tissue culture seedlings is promoted, but also the tissue culture seedlings are obviously thickened. After transplantation, the survival rate can reach more than 95%, and the tissue culture seedlings have high growth rate, great bud tillering rate, rapid growth rate and strong stress resistance. Compared with the control group of dendrobium officinale seedlings which are not inoculated to mycorrhizal fungi and bacteria to perform tissue culture, the dendrobium officinale seedlings inoculated to mycorrhizal fungi and bacteria have more prosperous root systems, obviously inflated plant internodes and high fresh weight increased by 10-20%.

The invention relates to control of sandy soil, in particular to a method and application for improving aeolian sandy soil by using peat in the southeast region of Horqin Sands. Peat is mixed in aeolian sandy soil so as to improve the texture, structure, moisture retention and organic content of the soil. After mixing, the aeolian sandy soil contains 2-8 percent of peat by weight percent. When the aeolian sandy soil with peat is used for planting celery cabbage, both production and quality of celery cabbage are increased, and plant height, dry weight, fresh weight and root length of celery cabbage are greatly increased.

The invention discloses a device and method for producing nuisanceless bean sprouts by adopting an extremely-low-frequency pulsed electric field. The device comprises an extremely-low-frequency high-voltage pulse generator, wherein two poles of the extremely-low-frequency high-voltage pulse generator are respectively connected with a copper plate; the two parallel copper plates are used as electrodes; and a nonmetal bracket for holding seeds is arranged between the two copper plates. The method comprises the following steps of: cleaning and sterilizing bean seeds or peanut seeds to be planted, killing surface microorganisms, soaking for 3-24 hours in water, putting water-absorbed and germinating seeds in the extremely-low-frequency pulsed electric field for treatment; and culturing the treated seeds under the normal temperature till to be harvested, and obtaining the nuisanceless bean sprouts. The device and method disclosed by the invention have the advantages that the obtained seeds treated and cultured in the pulsed electric field germinate quickly, the fresh weight, the sprout length and the like of seedlings are greatly different from those of the seedlings which are not treated by the pulsed electric field, and the quality and the yield are greatly increased.

The invention belongs to the technical field of flower culturing, especially the technical field of eustoma grandiflorum propagation. A method for accelerating germination of eustoma grandiflorum seeds is characterized by comprising the following steps of: placing the eustoma grandiflorum seeds in magnetic water containing 0.05-0.5 mmol/L of salicylic acid to soak for 2-3 hours, fetching out the seeds, drying, then placing the seeds in the magnetic water to soak for 2-3 hours, repeating the above operation 3-5 times, wherein the total soaking time is 12-16 hours; the seed soaking conditions are as follows: the temperature is 23-27 DEG C; and the illumination is 2000-2500 Lx. The method in the invention obviously accelerates germination trend and germination ratio of the eustoma grandiflorum seeds; and the complete fresh weight of the seedling is obviously larger than that of the control group. The magnetic water is added with the salicylic acid so as to accelerate the growth of the adventitious roots so that the root system is relatively developed, and the survival rate of the seedling after being transplanted is largely improved.

The invention discloses a land surface plant canopies ecological water content remote sensing retrieval method based on spectral analysis. The method comprises the following steps: (1) actually measuring plant canopies spectrum data and biochemical data, wherein the biochemical data comprises fresh weight, dry weight and leaf area; (2) preprocessing the actually measured spectrum data and performing spectrum feature extraction; performing moisture noise removing, spectrum differential computation and envelope line removing; (3) computing and extracting the required plant index according to theactually measured reflecting spectrum feature; (4) preforming correlation analysis on the plant field actually measured spectrum data and the biochemical data, and establishing the model of the computed plant index and the plant water content; and (5) analyzing the remote-sensing image spectrum feature, and realizing the plant canopies ecological water remote-sensing quantitative retrieval by combining the plant water content model established through the actually measured data. By utilizing the method disclosed by the invention, the dynamic monitoring on the plant canopies ecological water content can be realized, and the monitoring accuracy is high.

The invention provides a method for implanting and cultivating Gracilaria bursa-pastoris in a north pond. By using the method, the problems of water eutrophication and the like, caused by the ecological imbalance owning to using the prior art for signal animal breeding in the north pond, can be solved. The cultivation process of the invention can be divided to a sprout proliferation phase, a bottom sowing propagation phase, an increasing interharvesting phase and an overwintering seedling protection phase. By using the invention, the problem that owning to the change of factors such as climate, growing environment and the like in the north, the transplanting of south macrophyte fails, can be solved; the method has effects of low input and high output, and the ratio of the fresh weight input to fresh weight output of the algae is 1:1500. The yield per mu is 1500kg. The method of the invention can be used to fill the gap of cultivating macrophyte in aestival pond of northern areas and the diversity of algae resources in northern China is enriched.

The invention relates to a method for investigating lake benthophyte. The method comprises the steps of collecting and generating an image of the measuring area of a lake by using a double-frequency identification sonar, calculating the coverage degree of the benthophyte in the measuring area according to the image, conducting quadrat measurement for the measuring area to obtain the types of the benthophyte, the proportion of different types of benthophyte and the fresh weight density in the quadrat, obtaining the biomass of the benthophyte of a certain type in the measuring area by the totalarea of the measuring area * the coverage degree of the benthophyte in the measuring area * the fresh weight density in the quadrat * the proportion of the benthophyte of the type in the quadrat basedon the coverage degree of the benthophyte in the measuring area and the types of the benthophyte, the proportion of different types of benthophyte and the fresh weight density in the quadrat, and then obtaining the biomass of different types of benthophyte in the measuring area. The lake ecological investigation efficiency and the result accuracy can be improved. The method can be widely appliedto the investigation, treatment, and repair of the lake ecological environment.

The invention relates to an epulorhiza sp. strain S1 (CGMCC No.8145) and application of the epulorhiza sp. strain S1 in promoting of the growth of dendrobium officinale and the accumulation of dendrobium officinale polysaccharides. According to the application, a dendrobium officinale tissue culture seedling is taken as a material and is manually inoculated with the S1 (epulorhiza sp.) strain, after the tissue culture seedling and the S1 strain are co-cultured for 60 days, S1 fungus and the root of a host form a symbiotic relationship, the fresh weight increment, dry weight, plant height, stem height, stem diameter, number of nodes, internode length, number of tillers, number of fresh roots and polysaccharide content of the tissue culture seedling are remarkably higher than a contrast, and the polysaccharide content is 141.63% higher than the content of an uncultured seedling and is about 2.4 times of the polysaccharide content of the uncultured seedling. By utilizing a novel technique, the growth of the dendrobium officinale tissue culture seedling is greatly promoted, and the biomass and the polysaccharide content of the dendrobium officinale tissue culture seedling are increased; according to a technical method, the operability is strong, the high-quality dendrobium officinale tissue culture seedling can be produced, and high economic benefits are brought to an efficient cultivation technique and industrialization production of the dendrobium officinale.

The invention discloses a herbicide composition for preventing/killing farmland weeds, and application thereof, and in particular relates to herbicide composition for preventing and controlling gramineae weeds and broad-leaved weeds in cornfields. The composition is a three-element herbicide composition taking Nicosulfuron, Atrazine and Fluroxypyr as active components. The weight ratio of the Nicosulfuron to the Atrazine to the Fluroxypyr in the composition is 1-50:1-80:1-50, preferably 1-10:1-50:1-20. The composition has the advantages of having reasonable components, prevention and control effects superior to the activity when the components are applied separately, synergistic effect and high safety to the crops, expanding weed-controlling spectrum, reducing the frequency of pesticide application, reducing prevention and control cost, slowing down the resistance generation of the weeds and meeting the requirement of pesticide preparations on safety. The fresh-weight prevention and control effect of the composition on gramineae weeds and broad-leaved weeds in cornfields reaches 85.80 to 95.13 percent.

The invention provides a method for rapidly identifying and selecting salt-tolerant peanut varieties in a room. The method comprises the following steps of: 1) preparing saline solutions at concentrations of 0.15%, 0.30% and 0.45% by using NaCl, and applying the saline solutions in soil of potted plants; 2) selecting and sowing a to-be-tested peanut variety or a strain in potted plant pots, and observing and recording sprouting and growth conditions; 3) harvesting plants in 40 days after the sowing, and measuring the heights of the plants, the number of leaves, the dry and fresh weight of the part above the ground, and the dry and fresh weight index under the ground; 4) calculating a relative value of each index, carrying out clustering analysis on each salt-tolerant index, and determining the salt tolerance of each to-be-tested peanut variety or strain. By adopting the method combining indoor cultivation, seed germination and seedling growth conditions, the method for rapidly identifying and selecting salt-tolerant peanut varieties in the room can finish the indoor selection of salt tolerance of large batches of varieties or genes conveniently, rapidly and efficiently, is simple in operation, short in period, low in cost and high in identification efficiency, and has relatively strong operability and repeatability.

The invention discloses a bacteria strain FY4-2 selected from mass gen-seng endophytic bacterias; the bacteria strain FY4-2 produces auxin, azotification, phosphate dissolving, potassium releasing and ironophore, and has growth promotion function to the host gen-seng, and is identified to be enterobacterasburiae; the bacteria strain FY4-2 is strong in IAA production capacity, can reach 13.5 ug/mL, has good growth vigor under nitrogen-free condition, has azotification potential, phosphate dissolving content can be measured in quantity to be 130.6ug/mL, potassium releasing can obtain 4.33ug/mL, and can produce ironophore with activity at 92%; the bacteria strain FY4-2 can promote gen-seng seed to germinate and grow, bud length can be increased by 81.97%; the bacteria strain FY4-2 has obvious promotion effect for gen-seng root growth, fresh weight can be increased by 40.5%, and dry weight can be increased by 43.97%; the bacteria strain FY4-2 is found to provide excellent basis for developing and using gen-seng growth promotion bacterial manure.

The invention relates to an asparagus pond culture method and belongs to the technical field of alga culture. The substrate of a pond is selected, a water inlet and outlet system is arranged, and seawater is directly introduced into the pond; a culture raft frame is arranged in the pond, the row length of a single culture raft is 30-50 m, and the direction of the rafts is parallel to the water inlet and outlet direction; each culture raft comprises mooring ropes, seedling ropes and a floating ball, wherein the floating ball and the seedling ropes are tied onto the mooring ropes, the line spacing of the mooring ropes in the culture raft is 2.5-4.5 m, 30-40 seedling ropes are hung on each row of single raft, and the space between the rafts is 1-4 m. Asparagus breeding alga seedling clamping is conducted, the distance between the alga body and the water surface is adjusted to be 20-70 cm during culture, and the alga body is washed in a swing mode; when the fresh weight of asparagus on each seedling rope reaches 4-6 kg, 1/2-3/4 weight of asparagus is harvested, and the rest continues to grow. By the adoption of the method, high-yield culture of asparagus in northern ponds is achieved, and the defect that asparagus can only be cultured in an open offshore area is overcome.

The invention discloses a fast breeding method for dendrobium candidum protocorm tissue culture. The method comprises the following steps that 1, explants are cleaned and sterilized; then, the explants are inoculated into an induction medium to perform induction culture; 2, the protocorm inducted in the first step is inoculated into a liquid proliferation culture medium to perform suspension shaking culture; 3, the protocorm obtained in the second step is transferred into a differentiating culture medium to be cultured for obtaining differentiated seedlings; 4, the seedlings with the height being 1 to 2cm in the third step are transferred into a rooting culture medium for rooting. The method has the advantages that the proliferation times can be improved; the industrial fast breeding can be realized. The dendrobium candidum protocorm cultured by the method has high differentiation rate; the dry weight and fresh weight content is improved; the transplanting survival rate is correspondingly improved.