566 results about "Fresh weight" patented technology

The invention discloses a PgEGY3 gene for improving cold resistance of pennisetum alopecuroides and increasing fresh weight of leaves and application of the PgEGY3 gene, and relates to the technical field of genetic engineering, and the nucleotide sequence of the PgEGY3 gene of the pennisetum alopecuroides is shown as SEQ ID NO.1. The invention also discloses a protein coded by the Pennisetum alopecuroides PgEGY3 gene. The invention also discloses a recombinant expression vector and a recombinant host cell comprising the Pennisetum alopecuroides PgEGY3 gene. The invention also discloses application of the PgEGY3 gene, a recombinant expression vector and a recombinant host cell in improving cold resistance and biomass of pennisetum alopecuroides. It is verified that the Pennisetum alopecuroides PgEGY3 gene can improve the cold resistance of plants, and the growth state of the plants under cold stress is remarkably improved through overexpression of the PgEGY3 gene.

The invention provides a Kitasatospora xanthophycea JY01 strain and application thereof, the strain is separated from intestinal tracts of spring silkworms, has good disease-resistant and growth-promoting effects, and is specifically expressed in that cellulase, beta-1, 3-glucanase, siderophore and auxin IAA can be secreted; pot experiments show that the biocontrol efficiency of the microbial inoculum to wheat stalk rot is 57.90%, the biocontrol efficiency of the microbial inoculum to corn stalk rot is 46.41%, and the plant height and fresh weight are both significantly increased. The microbial agent is expected to be applied to preparation of biological bacterial fertilizer for effectively preventing and controlling soil-borne fungal diseases.

The invention belongs to the technical field of agricultural microorganisms, and particularly relates to pseudomonas fluorescens as well as a microbial agent and application thereof. The invention discloses a pseudomonas fluorescens TJ194, which is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.33697. The pseudomonas fluorescens TJ194 has the advantages that the pseudomonas fluorescens TJ194 can be used for preparing the pseudomonas fluorescens TJ194, and the pseudomonas fluorescens TJ194 can be used for preparing the pseudomonas fluorescens TJ194; tests prove that the strain has the activity of inhibiting the growth of fusarium pseudograminearum; by applying a fungicide containing the strain, the disease index of the wheat stem rot is remarkably reduced, and meanwhile, the fresh weight of the overground part and the underground part of wheat is increased, so that the pseudomonas fluorescens TJ194 and the fungicide thereof can be applied to prevention and treatment of the plant stem rot and promotion of plant growth.

The invention discloses an FtACA13 gene derived from tartary buckwheat as well as an expression vector and application of the FtACA13 gene. According to the invention, the FtACA13 gene is separated from tartary buckwheat; the polynucleotide sequence of the FACA13 gene is as shown in SEQ ID NO.1, and the amino acid sequence of the encoded protein of the FACA13 gene is as shown in SEQ ID NO.2. The FtACA13 gene is genetically transformed into buckwheat to obtain transgenic buckwheat hairy roots with the overexpressed FtACA13 gene, compared with hairy roots of a control group, the growth vigor and fresh weight of the fagopyrum tataricum hairy roots with the overexpressed FtACA13 gene under salt stress are increased, Na < + > accumulation in a plant body is remarkably low, it is proved that the FtACA13 gene can regulate and control the salt stress resistance of the plant, and the salt stress resistance of the fagopyrum tataricum hairy roots is improved. The invention has application prospects in the aspects of cultivating or breeding salt stress resistant plant varieties and the like.

The invention relates to the technical field of agricultural biological prevention and control, in particular to a pseudomonas chlororaphis subsp. Aurantiaca strain for preventing and controlling wheat basal stem rot and application of the pseudomonas chlororaphis subsp. Aurantiaca strain. The pseudomonas chlororaphis subsp. Aurantiaca strain for preventing and treating wheat stem rot is P. chlororaphis subsp. Aurantiaca, has very good antagonistic activity to fusarium pseudograminearum and fusarium flavum, the inhibition rate to the fusarium pseudograminearum strain reaches 76.07%, the inhibition rate to the fusarium flavum strain reaches 73.26%, and the inhibition rate to the fusarium flavum strain reaches 76.07%, so that the pseudomonas chlororaphis subsp. Aurantiaca strain for preventing and treating wheat stem rot can be used for preventing and treating wheat stem rot. The P. chlororaphis subsp. Aurantiaca can induce wheat to generate resistance to Fusarium pseudograminearum and Fusarium fulvum, the prevention and treatment effect on the Fusarium pseudograminearum strain reaches 55.77%, the prevention and treatment effect on the Fusarium fulvum strain reaches 51.19%, therefore, the P. chlororaphis subsp. Aurantiaca can significantly reduce the severity of the wheat basal stem rot, and the P. chlororaphis subsp. Aurantiaca can be used for preventing and treating the wheat basal stem rot. Meanwhile, the plant height, fresh weight and root length of wheat can be increased, and a remarkable growth promoting effect on wheat plants is achieved.

The invention discloses an application of a GmAIR12-2 protein in improving the low-phosphorus stress resistance of leguminous plants. According to the application disclosed by the invention, under low-phosphorus stress, GmAIR12-2 protein is excessively expressed in soybean plants, so that the root growth vigor of the soybean plants can be improved, the number and fresh weight of small root nodules of the soybean plants can be increased, the dry weight of overground parts and root systems of the soybean plants can be increased, and the total root length and the root surface area can be increased; it is shown that the protein can improve the tolerance of soybeans to low-phosphorus stress and relieve adverse effects of low-phosphorus stress on soybean growth and the like. Therefore, the invention provides the application of the GmAIR12-2 protein in improving the low-phosphorus stress resistance of the leguminous plants, and the protein can also be used for cultivating low-phosphorus stress resistant transgenic leguminous plant varieties, so that the leguminous plants planted in soil with relatively low available phosphorus content can also grow normally, and the yield of the leguminous plants is improved. Meanwhile, the invention also provides an important gene resource for breeding leguminous plant varieties resistant to low phosphorus stress.

The invention discloses preparation of a trichoderma composite flora and application of the trichoderma composite flora in prevention and control of crop fusarium wilt and improvement of soil, and relates to the field of agricultural production, and the composite flora is prepared by mixing trichoderma asperellum (5 * 10 < 7 > CFU / L), streptomyces (108 CFU / L), Chinese monospora (108 CFU / L), ensifer adhesion (108 CFU / L), pseudomonas nitroreducens (108 CFU / L) and paenibacillus alvei (108 CFU / L) according to the volume ratio of 6: 1: 1: 1: 1: 1: 1: 1 (V: V). According to the composite flora in the scheme, the composite flora is used for promoting growth of cucumber plants, and compared with a clear water control group (Foc), the fresh weight of cucumbers is increased by 55.51%, the stem diameter is increased by 12.76%, and the plant height is increased by 34.25%. The composite flora can also effectively prevent and treat fusarium wilt of cucumbers, and the prevention and treatment effect reaches 71.43%. Compared with the Trichoderma asperellum FJ035 treated group, the Trichoderma asperellum FJ035 has the advantages that the Trichoderma asperellum FJ035 is better than the Trichoderma asperellum FJ035 which is independently applied in the aspects of promoting the growth of cucumber plants and preventing and treating cucumber fusarium wilt, and compared with the Trichoderma asperellum FJ035 treated group, the control effect is improved by 56.25%. Meanwhile, the composite flora can be used for improving soil and preventing and treating multi-crop fusarium wilt.

The invention discloses a bacillus subtilis strain and application thereof, the bacillus subtilis strain is bacillus subtilis X-NY1 and is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC NO: M 20242038. Bacillus subtilis X-NY1, bacillus velezensis and bacillus amyloliquefaciens are compounded into a microbial agent, a film-forming agent, a dispersing agent, a coloring agent, a stabilizing agent, a preservative, a conditioning agent and a nutritional agent are further added to prepare the compound microbial seed coating agent, the effective viable count of the microbial agent is 108-1011, and the quality guarantee period is longer than 6 months. The compound microbial seed coating agent can improve the plant height, root length, fresh weight and the like in the growth process of pennisetum alopecuroides, can effectively improve the stress resistance of the pennisetum alopecuroides under the salinization condition, and is small in addition amount, low in cost, safe, reliable, obvious in growth promoting effect and suitable for application and popularization.

The invention discloses a multifunctional peoriae paenibacillus and application thereof, and relates to the technical field of microorganisms, and the key point of the technical scheme is as follows: Paenibacillus peoriae S6330 is separated from rhizosphere soil; the peoriae paenibacillus has a good inhibition effect on pathogenic bacteria, pathogenic fungi and oomycetes; meanwhile, the peoriae paenibacillus has the functions of dissolving potassium, producing protease, producing amylase, producing cellulase, producing siderophore and the like, has a growth promoting effect on various crops such as wheat, corn, watermelons, small oilseed rapes, tobaccos and the like, and can remarkably improve indexes such as chlorophyll content, plant height, fresh weight, overground part weight, underground part weight and the like of plants; in addition, the peoriae paenibacillus can be used for effectively inhibiting the tobacco bacterial wilt; the peoriae paenibacillus has no environmental pollution problem, is simple in culture condition, and has wide disease prevention and growth promotion application prospects in agricultural production.

The invention discloses a microbial agent and application thereof in preventing and treating rice blast and promoting rice growth, active ingredients of the microbial agent are formed by compounding cold bacillus with the preservation number of CGMCC (China General Microbiological Culture Collection Center) NO: 33492 and bacillus velezensis with the preservation number of CGMCC NO: 33493, and the microbial agent aims at solving the synergistic problem of preventing and treating rice blast and promoting rice growth. According to the complex microbial inoculant, through the synergistic interaction between the two strains, the metabolite of the complex microbial inoculant can completely inhibit the spore germination of magnaporthe oryzae; meanwhile, the fungicide can degrade cellulose, obviously promote the growth of plant height, fresh weight and dry weight of rice, and realize the integration of disease prevention and growth promotion. The invention further discloses a preparation method of the fungicide and an application method of the fungicide for preventing and treating rice blast and promoting growth in a root irrigation mode in the three-leaf stage of rice. The microbial agent disclosed by the invention has dual functions of efficiently preventing diseases and stably promoting growth, and an efficient and safe biological solution is provided for green and sustainable development of agriculture.

The invention relates to the technical field of plant genetic engineering, and discloses application of a soybean low-phosphorus-tolerant gene GmAP2 to improvement of low-phosphorus stress tolerance of plants. Research finds that under the condition of low phosphorus stress, the expression level of the GmAP2 gene in plants is remarkably increased. The GmAP2 gene is subjected to heterologous overexpression in arabidopsis thaliana, so that stress on plants under a low-phosphorus condition can be effectively relieved, the fresh weight and lateral root length of the arabidopsis thaliana can be remarkably increased, and the growth of the arabidopsis thaliana plants is promoted. In addition, under the low-phosphorus condition, overexpression of the GmAP2 gene can improve the phosphorus deficiency symptom of the soybean, improve the absorption of phosphorus nutrients by plants and promote the growth of plant roots and leaves. Therefore, the GmAP2 gene plays a very important role in dealing with the phosphorus starvation stress environment by the plant.

The invention relates to the technical field of saline-alkali soil improvement and microorganisms, in particular to bacillus pumilus S3-9 and application thereof. The bacillus pumilus S3-9 is preserved in the China General Microbiological Culture Collection Center on December 19, 2024, and the preservation number of the bacillus pumilus S3-9 is CGMCC (China General Microbiological Culture Collection Center) No. 33120. The bacillus pumilus S3-9 provided by the invention has the effects of reducing salinity in saline-alkali soil, increasing nutrients of the saline-alkali soil and improving the diversity and stability of rhizosphere microbial communities of the saline-alkali soil, and can be applied to improving soil fertility, promoting nutrient circulation, improving soil quality, relieving soil salinization and the like. The strain can also be used for increasing the yield of sweet sorghum in saline-alkali land, has a remarkable growth promoting effect on plant height, overground part fresh weight and underground part fresh weight, can effectively improve the K < + > / Na < + > ratio, chlorophyll content, superoxide dismutase activity and catalase activity, and is beneficial to enhancing the adaptive capacity of plants to salt stress.

The invention discloses a cold response PaIBH1 protein and application of a coding gene thereof in enhancing cold stress resistance of plants. The cold response PaIBH1 protein is a protein PaIBH1 with an amino acid sequence as shown in SEQ ID NO: 2. Experiments prove that the gene for coding the protein PaIBH1, namely the gene PaIBH1, is overexpressed in wild type arabidopsis thaliana, so that the cold resistance of the arabidopsis thaliana can be improved; the improvement of the cold resistance shows that after cold treatment, the fresh weight of the overground part is increased, the wilting rate is reduced, the leaf membrane damage and electrolyte leakage are reduced, the photosynthetic ability is improved, the membrane lipid peroxidation damage is relieved, the active oxygen scavenging ability is enhanced and / or the active oxygen accumulation in the plant body is reduced. Therefore, the protein PaIBH1 can improve the cold resistance of the plants. The method has a very wide application prospect in the aspect of cold-resistant genetic improvement of crops or pasture.

The invention discloses an SSR (simple sequence repeat) molecular marker associated with fresh weight character of corydalis tuber bulbules as well as primers and application of the SSR molecular marker, and belongs to the technical field of molecular genetics. According to the SSR molecular marker associated with the fresh weight character of the corydalis tuber bulbous disclosed by the invention, the amplification primer sequences of the SSR molecular marker are shown as SEQ ID NO.1 and SEQ ID NO.2. The SSR molecular marker associated with the fresh weight character of the rhizoma corydalis coleobulb and the amplification primer thereof can be applied to rhizoma corydalis population structure analysis, rhizoma corydalis coleobulb fresh weight character associated marker analysis, rhizoma corydalis excellent germplasm breeding and preparation of a kit for screening rhizoma corydalis excellent germplasm with high coleobulb weight. The method can be directly used for excellent germplasm screening, gene localization and cloning and molecular marker-assisted breeding, has chromosome localization information, is beneficial to further fine localization and cloning of genes for controlling fresh weight characters of corpus balls, and has important significance in breeding of high-yield rhizoma corydalis.

The invention discloses a method and system for predicting plant photosynthetic ability by using plant leaf traits, and the method comprises the following steps: obtaining fresh weight, actual leaf area, specific leaf weight and relative water content of leaves, measuring leaf nitrogen content and leaf carbon content of the leaves, obtaining unit area leaf nitrogen content, unit area leaf carbon content and leaf carbon nitrogen ratio of the leaves, and determining the plant photosynthetic ability according to the unit area leaf nitrogen content, the unit area leaf carbon content and the leaf carbon nitrogen ratio of the leaves. And calculating the photosynthetic capability index of the plant to which the leaf belongs based on the obtained leaf character data and the prediction linear model of the leaf character photosynthetic capability parameter. By adopting the scheme, in-situ determination work does not need to be carried out in the field, the workload of technicians is greatly reduced, the working process and the operation difficulty of field operation are simplified, meanwhile, the measurement precision and the accuracy of a measurement result are considered, a large number of samples can be synchronously processed and determined, and the measurement efficiency is improved. The photosynthetic capacity parameters of various plants can be obtained in the same time period, and meanwhile, the dependence on the number of photosynthetic instruments in the photosynthetic in-situ determination process is avoided.

The content of IAA produced by the enterobacter ludwigii XL0404 can reach 18.94 mg / L, the content of ACC deaminase produced by the enterobacter ludwigii XL0404 is 484.48 U / L, and the enterobacter ludwigii XL0404 has multiple functions of dissolving phosphorus, dissolving potassium, fixing nitrogen, producing siderophore and the like, has an inhibition effect on main root and stem disease pathogenic bacteria such as phytophthora nicotianae, rhizoctonia solani, rhizoctonia tectorum, fusarium oxysporum, fusarium solani and the like, and has an inhibition rate of 41.20-55.88%; the germination rate of the tobacco seeds is increased through treatment of the strain XL0404, and the root length is remarkably promoted; the root activity, the whole plant fresh weight, the overground fresh weight, the root fresh weight, the maximum leaf length and the maximum leaf width of the tobacco seedlings treated by the strain XL0404 with the bacterium content of 1 * 10 < 7 > cfu / mL in a seedling culture substrate are obviously higher than those of the tobacco seedlings treated by contrast treatment, and the strain XL0404 can be used as plant growth-promoting rhizobacteria and has a good application prospect in research and development of microbial fertilizers and biological prevention and control of tobacco rhizome diseases.

The invention belongs to the technical field of plant molecular breeding, and particularly relates to an LpBPM5.6 gene for regulating and controlling a perennial ryegrass plant type, a low expression vector, a construction method and application of the LpBPM5.6 gene. The invention discloses a perennial ryegrass LpBPM5.6 gene for regulating and controlling a plant type. The nucleotide sequence of the perennial ryegrass LpBPM5.6 gene is shown as SEQ ID No. 1. According to the invention, a gene full-length sequence, namely LpBPM5.6, for regulating and controlling the perennial ryegrass plant type is cloned for the first time. The LpBPM5.6, as a gene for regulating and controlling the plant type of perennial ryegrass, can enable the perennial ryegrass to present an ideal plant type. The inventor constructs an LpBPM5.6 low expression vector to obtain a ryegrass plant with low expression of the LpBPM5.6 gene. Results show that compared with wild perennial ryegrass, after low expression of the gene, the tiller number of the perennial ryegrass can be increased by more than 20%, the plant height and fresh weight can be reduced by more than half, fibrous roots are obviously reduced, and obvious changes of the characters provide support for plant type improvement of turfgrass such as the perennial ryegrass.

The invention provides a growth-promoting rhizobacteria for enhancing salt tolerance of corn and application of the growth-promoting rhizobacteria, and belongs to the technical field of agricultural microorganisms. The invention provides a pseudomonas bacterium TJ1-185, and the preservation number of the pseudomonas bacterium TJ1-185 is CDMCC (China Description of Microbiological Culture Collection Center) No.64979. The pseudomonas bacteria TJ1-185 can improve the salt stress resistance of plants and relieve the damage of salt stress to the plants. The result of the embodiment shows that the pseudomonas bacterium TJ1-185 can promote corn to germinate under salt stress, improve the plant height, root length, overground part fresh weight, root fresh weight, overground part dry weight and root dry weight of corn under the salt stress condition, and reduce the malondialdehyde content, thereby promoting the growth of corn under the salt stress condition and improving the salt stress resistance of corn. The damage of salt stress to the corn is relieved.

The invention discloses a method for evaluating and screening salt tolerance of triticale germplasm resources in a germination stage, and belongs to the technical field of evaluation and screening of plant salt tolerance, salt stress concentration screening: triticale seeds are treated by NaCl solutions with different concentrations, indexes such as survival rate, plant height and overground part fresh weight are measured, the NaCl solution with the concentration of 200 mmol. L <-1 > is determined as the salt tolerance evaluation appropriate concentration, and the salt stress concentration is determined as the salt tolerance evaluation appropriate concentration. Salt tolerance evaluation test: carrying out salt stress treatment by adopting a 200mmol. L <-1 > NaCl solution, determining phenotypic indexes such as survival rate, plant height, fresh weight of overground part, root length and chlorophyll content, and physiological and biochemical indexes such as catalase, peroxidase and superoxide dismutase activity and malondialdehyde content, and carrying out principal component analysis, membership function analysis and clustering analysis to obtain a salt tolerance evaluation result. Calculating a salt tolerance comprehensive evaluation value, and screening salt tolerance triticale germplasm resources.

The invention relates to a growth-promoting compound microbial agent, which is prepared from bacillus pseudomycoides, bacillus subtilis and Trichoderma longibrachiatum, and is characterized in that the growth-promoting compound microbial agent is prepared from bacillus pseudomycoides, bacillus subtilis and Trichoderma longibrachiatum, and the growth-promoting compound microbial agent is prepared from bacillus amyloliquefaciens, bacillus subtilis and Trichoderma longibrachiatum. The compound microbial agent (bacillus subtilis, pseudofungal bacillus and trichoderma longibrachiatum) has excellent performance in the aspects of promoting plant growth and improving the soil environment, and the plant height, the fresh weight and the root length of plants are remarkably increased. The application of the complex microbial inoculant effectively relieves the chive continuous cropping obstacle, improves the chive growth potential and soil fertility, and provides a theoretical basis and technical support for sustainable planting of chives in facility agriculture.

The invention discloses a biocontrol bacterium for preventing and treating soybean phomopsis longicolla caused by the phomopsis longicolla and application of the biocontrol bacterium, the biocontrol bacterium is named as Aspergillus qilianyuensis JSNL-ZJ66, the preservation unit is China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC NO.41157. The invention further discloses a preparation method of the biocontrol bacterium for preventing and treating the soybean phomopsis longicolla caused by the phomopsis longicolla. The strain is mainly applied to prevention and control of soybean pith seed rot caused by P.lon gilcola, the severity of the soybean pith seed rot can be remarkably reduced, and growth indexes such as the budding rate, the plant height and the fresh weight of the soybean pith seed rot are remarkably improved.

The invention relates to bacillus velezensis CGMCC (China General Microbiological Culture Collection Center) No.32593 as well as preparation and application of a microbial agent of the bacillus velezensis, and belongs to the technical field of microorganisms. The strain disclosed by the invention has the effects of dissolving phosphorus, dissolving potassium, fixing nitrogen and producing indoleacetic acid (IAA) at the same time. The method comprises the following steps: inoculating bacillus velezensis CGMCC No.32593 into an LB liquid culture medium, and fermenting by using a 5L fermentation tank to obtain the liquid microbial agent. The microbial agent provided by the invention is simple in preparation method and convenient for industrial production. By applying the liquid microbial agent, the plant height of tomatoes and the fresh weight of overground parts can be effectively increased, and the utilization rate of soil phosphorus in crop production is increased.

The invention belongs to the technical field of vegetable planting, and particularly relates to a seedling treatment composition and application thereof in improving stress resistance of solanaceous vegetables and promoting growth of the solanaceous vegetables. According to the method, solanaceous vegetable seeds are soaked in the seed treatment liquid obtained by dissolving the seedling treatment composition in water, the soaked seeds are subjected to dark culture, and the seedling treatment liquid is sprayed in the solanaceous vegetable seedling stage, so that the low temperature resistance of solanaceous vegetables can be effectively improved; the vigor of solanaceous vegetable seeds and the quality of seedlings under the low-temperature condition are improved. The test result shows that the seedling emergence potential, the seedling emergence rate and the seedling emergence index of the solanaceous vegetable seeds under the low-temperature condition can be improved and the average seedling emergence time can be shortened by using the seedling treatment liquid and culturing mode, and meanwhile, the chlorophyll content, the plant height, the root length and the fresh weight of the solanaceous vegetable seedlings under the low-temperature condition can be improved.

The invention discloses three growth-promoting bacteria combined with sinorhizobium freudenreanum and application thereof, and belongs to the technical field of microorganisms. According to the invention, three growth-promoting bacteria of fish pseudomonas ZMC01, pseudomonas puticola ZMC129 and cellulomicrobe vinckii ZMC94 are separated from the root of an oxytropis glabra plant in a saline-alkali beach, and the three growth-promoting bacteria have the ability to grow in a saline-alkali environment. Tests prove that the growth-promoting bacteria ZMC01, ZMC129 and ZMC94 can promote the growth of soybeans in a saline-alkali environment or a non-saline-alkali environment by independently acting or jointly acting with the sinorhizobium freudenreanum, and indexes such as plant height, root length, stem and leaf fresh weight, root fresh weight, root nodule number and root nodule fresh weight of plants are remarkably improved or increased. The growth-promoting bacteria can also improve the nodulation capacity of the sinorhizobium freudenreanum in a saline-alkali environment, the nodulation and nitrogen fixation capacity of the soybeans under saline-alkali stress is expected to be fundamentally improved, and the growth-promoting bacteria have important significance for guaranteeing safe production of the soybeans.

The invention discloses a nano-material complex microbial inoculant and application thereof, and relates to the technical field of microorganisms. The invention discloses application of a nano-material complex microbial inoculant in promoting growth of overground parts of plants, and results show that compared with a treatment group singly inoculated with YH-18, the plant height, leaf area, ground diameter, fresh weight, dry weight and chlorophyll content of tomato seedlings subjected to complex treatment are all remarkably increased. The invention discloses application of a nano-material complex microbial inoculant in promoting plant root growth, and results show that the root length, the root surface area and the root tip number are respectively increased by 35.76%, 13.19% and 57.84% compared with those of single inoculation of YH-18. After compounding and inoculating, the content increase of rapidly available potassium, rapidly available phosphorus and hydrolytic nitrogen in the soil is effectively promoted, the content of organic matters in the soil is remarkably increased, and the nutrient structure of the soil is improved; pseudomonas is significantly enriched through composite inoculation, and the abundance of TRA3-20 is significantly reduced.

The invention discloses an enterobacter strain and application thereof in improving stress resistance of plants. The invention relates to the field of biological agriculture, and provides Enterobacter sphaeroides YJD-AD2-2, and the enrollment number of the Enterobacter sphaeroides YJD-AD2-2 in the General Microbiological Culture Collection Center of the China Committee for Culture Collection of Microorganisms is CGMCC No.29524. The Enterobacter sphaeroides YJD-AD2-2 can be used for preparing the enterobacter sphaeroides YJD-AD2-2. The enterobacter sphaeroides YJD-AD2-2 provided by the invention can improve the resistance of arabidopsis thaliana, alfalfa, corn and rice to salt and alkali stress, specifically, the dry weight, fresh weight, plant length, leaf area and chlorophyll content are increased, the oxidation resistance is improved, wilt is reduced, and physiological and biochemical indexes are improved. The YJD-AD2-2 microbial inoculum improves the property and activity of saline alkali soil and improves the abundance and composition of microbial communities. Detection shows that the YJD-AD2-2 strain has the capabilities of secreting IAA (Indoleacetic Acid), ACC (Acetyl Cellulose) deaminase and siderophore, dissolving phosphorus and fixing nitrogen. The indexes show that the YJD-AD2-2 microbial inoculum has the potential of growth-promoting bacteria and has important application value in the field of agriculture.

The invention relates to a streptomyces sp. Strain EC15 and an application of the streptomyces sp. Strain EC15. The streptomyces strain EC15 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation address is No.3, yard 1, Beichen West Road, Chaoyang District, Beijing, the preservation date is December 25, 2024, and the preservation number is CGMCC NO.33195. The streptomyces strain EC15 has the advantages that the streptomyces strain EC15 can be used for preparing the streptomyces strain EC15; the strain has the functions of dissolving organic phosphorus, dissolving inorganic phosphorus, producing a biological membrane, producing an iron carrier, producing IAA and producing ACC deaminase. The EC15 bacterial liquid is used for soaking seeds under the stress of 200 mmol / L and 250 mmol / L NaCl, so that the germination potential, the germination rate, the germination index, the radicle length, the germ length and the fresh weight of group seedlings of the alfalfa seeds can be obviously improved. After the EC15 strain is inoculated, under the stress of 200 mmol / L NaCl, the plant height, stem diameter, leaf area and overground part fresh and dry weight of alfalfa plants can be obviously improved.

The invention relates to the technical field of microbial fermentation, and discloses an agricultural ecological probiotic complex microbial inoculant, which is prepared from the following components in percentage by mass: 30 to 35 weight percent of beneficial microbial inoculant, 20 to 25 weight percent of mineral source potassium fulvate, 15 to 20 weight percent of slow release agent, 10 to 15 weight percent of nano polypeptide fermentation liquor and the balance of carrier, the processing method comprises the following steps: S1, pretreating the raw materials; s2, preparing nano polypeptide fermentation liquor; s3, complex microbial inoculants are compounded; and S4, drying and forming. According to the invention, the mineral source potassium fulvic acid and the nano-polypeptide fermentation liquor are added, so that effective regulation and control of soil micro-ecology are realized; crop growth is promoted through cooperation of multiple strains, growth hormone is secreted through bacillus subtilis and the like, nitrogen is fixed through bacillus amyloliquefaciens, root development is stimulated in cooperation with mineral source potassium fulvic acid, and the plant height, stem diameter and fresh weight of crops are remarkably increased; the particle size of the polypeptide subjected to high-pressure homogenization treatment is 80-120nm, the absorption efficiency is improved by 60%, and a crop disease-resistant signal channel is activated.

The invention discloses application of N-acetyl-L-cysteine in improving drought tolerance of plants, and belongs to the technical field of plant cultivation. The invention provides the application of N-acetyl-L-cysteine (N-acetyl-L-cysteine, NAC) in the improvement of the drought tolerance of the brassica napus for the first time. Experiments prove that compared with a control group, the fresh weight, the dry weight, the root length and the chlorophyll content of rape seedlings subjected to foliage spraying of 150 mu M of N-acetyl-L-cysteine under the drought stress condition are improved, the water loss rate of leaves is reduced, and the drought stress tolerance of the rape is enhanced. Therefore, the drought stress tolerance of the rape treated by the N-acetyl-L-cysteine is obviously improved. Furthermore, an optimal application concentration of 150 mu M is obtained through experimental exploration, so that the N-acetyl-L-cysteine can be optimally utilized in improving the drought stress tolerance of the oilseed rape.

The invention belongs to the technical field of molecular biology, and particularly relates to application of an OsSCL25 gene in regulating and controlling salt stress resistance of rice. Under the stress of salt, the survival rate, chlorophyll, fresh weight and dry weight of strain seedlings of the overexpressed OsSCL25 gene are higher than those of a wild type, the content of superoxide dismutase, peroxidase and malondialdehyde is lower than that of the wild type, and the content of proline is remarkably higher than that of the wild type and a mutant. The soluble sugar content of the wild type, the soluble sugar content of the OsSCL25 mutant and the soluble sugar content of the overexpression system are not obviously different under normal treatment, and the soluble sugar content of the overexpression system is obviously higher than that of the wild type and the soluble sugar content of the gene mutant after salt stress, so that the OsSCL25 gene participates in rice salt tolerance regulation. After the gene is over-expressed, the salt tolerance of the rice is obviously improved, so that a rice material suitable for saline-alkali soil planting improvement can be cultivated by utilizing the OsSCL25 through a biotechnology, and a new thought is provided for improving the planting rate of the rice in the saline-alkali soil.