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Bacillus atrophaeus strain DPPG-28 and application in crop disease control

A technology of DPPG-28 and Bacillus atrophs, applied in the field of agricultural biology, can solve problems such as unsatisfactory effects, achieve good ecological and social benefits, strong adaptability, and improve the effect of plant height at seedling stage

Inactive Publication Date: 2015-04-22
BEIJING INSTITUTE OF TECHNOLOGYGY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, some commercialized biocontrol preparations at home and abroad are not effective in preventing and controlling some soil-borne diseases on crops in Xinjiang due to the above reasons. It is urgent to screen out some suitable for local soil and climate based on the unique microbial resources in Xinjiang. Conditional biocontrol bacteria are used for the control of crop diseases, which is of great significance both in terms of technical invention and practical application

Method used

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  • Bacillus atrophaeus strain DPPG-28 and application in crop disease control
  • Bacillus atrophaeus strain DPPG-28 and application in crop disease control
  • Bacillus atrophaeus strain DPPG-28 and application in crop disease control

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1 Isolation, purification and screening of disease-preventing and growth-promoting bacterial strains

[0017] (1) Isolation, purification and preservation of rhizosphere microorganisms

[0018] Weigh 10g of the soil samples collected from the processing tomato production area, add 100ml of sterilized water, then add an appropriate amount of glass beads and shake vigorously for 30min, then let it stand for 10min, take 1ml of the supernatant and add it to a triangle filled with 99ml of sterilized water. bottle, serially diluted to 10 -6 concentration. Add 200 μl of each of the above dilutions to PDA, NA, and Gao’s No. 1 medium plates, spread evenly with a spreader, and after cooling, place them upside down in an incubator at 28°C for cultivation, and repeat twice. After 3-7 days, single colonies of different shapes were picked from each dish and purified on the slant of the test tube for testing. Strains were preserved with distilled water preservation method. ...

Embodiment 2

[0034] Identification characteristics of embodiment 2 DPPG-28 bacterial strain

[0035] According to the morphological characteristics of the bacteria, the morphological characteristics of the colony and the results of Gram staining, combined with the eighth edition of Bergey's Manual of Etermitive Bacteriology and the "Manual of Common Bacteria Identification", the physiological and biochemical characteristics of the strain DPPG-28 were analyzed. identification. The results are shown in Table 3. After preliminary identification, the strain DPPG-28 belonged to the genus Bacillus.

[0036] Table 3 Physiological and biochemical characteristics of strain DPPG-28

[0037]

[0038] Note: 10: The reaction is positive or can be grown and utilized; 1: The reaction is negative or cannot be grown and utilized.

[0039] 16S rDNA Identification of Strain DPPG-28

[0040] The 16S rDNA of strain DPPG-28 was sequenced, and the size of the 16S rDNA sequence fragment of DPPG-28 obtained ...

Embodiment 3

[0041] The preparation of embodiment 3 Bacillus atrophaeusDPPG-28 biocontrol agent

[0042] (1) Preparation of seed solution

[0043] After the bacterial strain DPPG-28 obtained in Example 1 was inoculated on NA medium for activation, it was transferred to a 250mL Erlenmeyer flask containing 100mL NA medium, and cultivated at 30°C and 200rpm for 24 hours to obtain a seed solution.

[0044] (2) Preparation of bacterial agent

[0045] Prepare medium, beef extract 5.0g, peptone 10.0g, yeast powder 5.0g, NaCl 5.0g, glucose 10.0g, distilled water 1000mL, sterilize at 121℃ for 20min. The seed solution was inoculated in the fermentation medium at a ratio of 1:100, and cultured at 30°C and 200rpm for 48h. The concentration of viable bacteria in the fermentation broth was 10 8 -10 11 cfu / mL.

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Abstract

The invention discloses a bacillus atrophaeus strain DPPG-28 and an application in crop disease control, and belongs to the field of agricultural biotechnology. The strain is separated from soil of drip irrigation fields with damping off in tomato processing production areas, and has an accession number of CGMCC No. 9394. The strain can be used for biological control of crop pathogens such as rhizoctonia solani, pythium aphanidermatum, alternaria solani, phytophthora capsici, tomato scab fungi, and the like; the control effects on tomato damping off, capsicum early blight, and cotton damping off reach respectively 74.17%, 66.83%, and 70.12%; the strain can significantly improve the crop germination rate, plant height, fresh weight, and dry weight at the seedling stage. The strain of the invention has high-efficient wide-spectrum germicidal activity, and has wide application prospects in biocontrol agents.

Description

technical field [0001] The invention relates to a newly screened bacillus atrophaeus DPPG-28 and its application, belonging to the field of agricultural biotechnology. The object of the present invention is to provide a Bacillus atrophaeus DPPG-28 which can be used for the prevention and treatment of tomato wilt disease, has high-efficiency and broad-spectrum bactericidal activity, and can be used for the prevention and control of various crop diseases. Background technique [0002] Xinjiang is my country's largest cotton production area and high-quality commodity cotton base, and occupies a pivotal position in Xinjiang's economic production. In recent years, the chili processing industry has also gradually emerged in Xinjiang, occupying an important position in Xinjiang's economic production. In recent years, the tomato processing industry, known as the "red industry", has gradually become a pillar industry in Xinjiang. The planting area of ​​processed tomatoes in Xinjiang...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/07
CPCA01N63/00C12N1/205C12R2001/07
Inventor 李春武占省樊艳爽凃亮何艳慧
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
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