Method for manufacturing dendrobium officinale artificial seeds

Abstract

The invention provides a practical technique for breeding dendrobium officinale by using artificial seeds. The invention aims to provide a technique for breeding dendrobium officinale with high efficiency and low cost. The technique comprises the following steps of: inducing dendrobium officinale seeds on a germination culture medium to obtain protocorms, then performing propagation culture on the protocorms, transferring the protocorms to MS culture solution containing 0.5mg / L of ABA to perform shaking culture (100r / min), and replacing the culture solution once every 10 days; selecting the protocorms with length * width of about 0.5-1.5mm * 2.0-3.4mm as embedding propagation bodies by using a 6-mesh nylon mesh screen after 30 days, manufacturing the artificial seeds by adopting an instillation method, preparing basic artificial endosperm from MS, 0.5 mg / L of BA, 0.5mg / L of NAA, 0.3 percent of AC, 3 percent of sodium alginate and 0.3 percent of chlorothalonil, adding 1.5 to 2.0 percent of starch, 1.0 to 2.0 percent of water-retaining agent and 1.0 to 3.0 percent of nano TiO2 into the basic artificial endosperm, preparing artificial seed coat matrix by adding 2 percent of chitosan and 5.0 to 10 percent of nano TiO2 into sodium alginate, and performing germination and storage research of the artificial seeds.

Description

Technical field [0001] The invention belongs to the field of bioengineering technology. Specifically, it relates to a practical technology for using artificial seeds to breed Dendrobium officinale. Background technique [0002] Dendrobium candidum Wall.ex Lindl. is a plant of the orchid family Dendrobium. It is a traditional and precious Chinese medicine in my country. It ranks first among the "Nine Great Immortals of China". It has the effects of nourishing yin and clearing heat, promoting fluid and quenching thirst. Modern pharmacological research shows that it also has the effects of anti-tumor, anti-aging, strengthening the body's immunity, dilating blood vessels and anti-platelet aggregation, so it is widely used in clinical and Chinese medicine compound prescriptions. Dendrobium officinale is an epiphytic plant of the orchid family that grows slowly and has very low self-reproductive capacity. Due to its huge medicinal and health value, it has been predatory for a long tim...