4509 results about "Dendrobium nobile" patented technology

This invention relates to polysaccharides extract from Dendrobium candidum and its preparation method. The molecular weight of the polysaccharide extract of Dendrobium candidum is 10000-300000. The content of polysaccharide is no less than 25 wt%. The invention also relates to a Chinese medicinal composition containing polysaccharide extract from Dendrobium candidum and its preparation method, and application of the polysaccharide extract in preparing medicine for auxiliary treatment of diseases caused by hypoimmunity. The extract and Chinese medicinal composition containing the same can improve immunologic function with high bioavailability, no toxicity and adverse side effect. the preparation method is simple and convenient for operation and commercial process.

The invention discloses health tea which is manufactured by mixing 0 to 80 weight parts of herbal tea, 0 to 50 weight parts of multicolor tea and 0 to 10 weight parts of medicinal and edible nourishing medicinal material, wherein the herbal tea is manufactured by adopting one or more from prunus persica, flos rosae rugosae, tulipa, rosa chinensis, chrysanthemum, aloe, apple flower, hibiscus sabdariffa, jasminum sambac, lemon slices, lavandula pedunculata, lilium, lemon grass, prunus mume, trollius chinensis bunge, superslim, sterculia lychnophora, fiveleaf gynostemma herbs, stevia rebaudiana, radix notoginseng, osmanthus fragrans, jade beauty, stellaria alsine grimm, dianthus caryophyllus, apocynum venetum, cordyceps, flos lonicerae, paeonia lactiflora and arctium lappal; the multicolor tea is manufacrured by adopting one or more from green tea, black tea, dark green tea and white tea; and the medicinal and edible nourishing medicinal material is manufactured by adopting one or more from panax quinquefolius, radix glycyrrhizae, dendrobium nobile, salvia miltiorrhiza, cornus officinalis, radix pseudostellariae and codonopsis pilosula. The health tea has the advantages that fragrance, sweetness and mellowness of scented tea are maintained, a health care function of the nourishing medicinal material is further fulfilled, and besides, tea soup is rich in taste and is sweet, refreshing and fragrant.

The invention discloses a tree tissue culture method of dendrobium candidum, which belongs to the agriculture technical field, and aims at solving the technical problem that a tree tissue culture method of the dendrobium candidum is provided, and belongs to the bionics wild cultivation. In order to solve the problems, the tissue culture method of the dendrobium candidum comprises the following steps that: 1) a tree with surface of a tree bark having grains is selected as an epiphytic tree, a dendrobium candidum seedling after being domesticated is selected as an epiphytic seedling, and a hay band is selected as a binding rope; 2) the epiphytic seedling is arranged on the surface of a trunk of the epiphytic tree, and root of the epiphytic seedling is wound by the binding rope to be fixed; and 3) the tree tissue culture humidity is controlled at 70 percent to 85 percent. Due to the adoption of the method, the dendrobium candidum can be planted on the tree with the surface having the grains.

The invention discloses a method for tissue-culture breeding and wild-simulated cultivation of dendrobium candidum, which comprises: inoculating dendrobium candidum tender straw slices onto a culturemedium for culture under conditions of a temperature of 22 to 24 DEG C and an illuminance of 1,000 to 1,500 Lx to first induce the growth of calles to form protocorms and then promote the multiplicative growth of the protocorms into 5 to 8 centimeter strong seedlings; and moving the seedlings out of a culture room, hardening the seedlings at a normal temperature for 3 to 5 days and transplanting the seedlings into the ground in a green house. The plane basic layer of a cultivation check field in the green house are provided with spaced breather holes; the upper layer of the cultivation check field is a cultivation medium; the seedlings are planted at a row spacing of 12*8cm and a spacing in the row of 12*8cm; in the green house, the temperature is 0 to 28 DEG C, the humidity is 60 to 70 percent and the shade density is 70 to 80 percent; and disease and pest prevention and control are carried out periodically and the dendrobium candidum can be harvested in 3 to 4 years. The method improves the civil year breeding coefficient of the dendrobium candidum by about 10 times, provides enough seedlings for large-scale cultivation, solves the problem of wild-simulated cultivation and realizes scale cultivation.

The invention discloses a planting method for Dendrobium officinale. The method comprises the following steps of building a greenhouse, preparing a culture substrate, conducting transplanting, conducting field management, preventing and controlling plant diseases and insect pests, and conducting harvesting. According to the planting method for the Dendrobium officinale, the cultivation method for the Dendrobium officinale is standardized, significant technical problems and production problems, such as the problems of the substrate processing technology, the spraying technology and the pest control technology, existing in production are solved, the quality technology test and research are improved, and standardization production technical regulations are established.

The invention provides a tie-to-tree dendrobium officinale cultivation method, comprising the steps of seedling tissue-culture breeding, tissue-culture seedling acclimatization and tie-to-tree cultivation of acclimatized seedlings. The step of tissue-culture seedling acclimatization comprises selection of bottled tissue-culture seedlings, selection and arrangement of a seedling acclimatization medium, selection of seedling acclimatization fertilizer, planting and after-planting management and is characterized in that the seedling acclimatization medium consists of hardwood sawdust and broken stone mixed in a mass ratio of 6.5-7.5:2.5-3.5, and the seedling acclimatization fertilizer is organic fertilizer prepared by fermenting herbivorous animal waste. The step of tie-to-tree cultivation of acclimatized seedlings comprises selection of acclimatized seedlings, selection of cultivation forest land, selection of variety of trees to be tied, seedling tying up and planting management. The tie-to-tree dendrobium officinale cultivation method provided by the invention has the advantages of low planting cost, easiness in management, high survival rate and long harvesting period; moreover, the harvest products have high quality.

The invention discloses a Dendrobium officinale culture solution and belongs to the technical field of agricultural planting. The culture solution comprises the following ingredients: potassium nitrate, ammonium nitrate, magnesium sulfate, manganese sulfate, zinc sulfate, monopotassium phosphate, copper sulfate, potassium iodide, cobalt dichloride, boric acid, sodium molybdate, vitamin VB1, vitamin VB6, vitamin VB5, glycine, ferrous sulfate, disodium ethylenediamine tetraacetic acid, calcium chloride, naphthylacetic acid, mashed banana, mashed potato, white sugar, inositol, and powdered agar. The dendrobium officinale culture solution is reasonable in compatibility, can provide comprehensive nutrition for a Dendrobium officinale stem, fast promote the stem to grow out of a root, increase the polysaccharide content and the amino acid content in the Dendrobium officinale, shorten the time for culturing the Dendrobium officinale stem to a complete plant to about 25 days, and significantly improve the transplanting survival rate of the Dendrobium officinale.

The present invention discloses a prescription for treating gastropathy, pertaining to a Chinese medicine. The recipe includes 10 chunhua, 10 finger citron flower, 10 official magnolia flower, 10 green tangerine peel, 10 zhixiangfu, 10 heterophylly falsestarwort root, 15 fried largehead atractylode with earth, 6 prepared pinellia tuber, 12 white poria, 10 dried citrus peel, 6 liquorice, 10 gongying, 12 bullbrier, 10 lobed kudzuvine, 10 hovenia dulcis, 15 parched Chinese radish seed, 10 glabrous greenbrier rhizome, 12 white peony root, 10 Chinese angelica root, 15 radix adenophorae, 12 dwarf lilyturf , 10 nobile dendrobium, 6 bamboo shavings, 10 virgate wormwood herb, 6 bupleurum falcatum, 6 parched gentian with wine, 10 ground ivy, 6 jinlingzi, 10 xuanhusuo, 6 betelnut, 10 zhichuanjun, 15 chicken's gizzard skin, 15 fried barley sprout and 10 citrus medica, as well as three pieces of gingers and four jujubes as pharmacal. The prescription has good curative effect on superficial gastritis, atrophic gastritis, erosive gastritis, hypertrophic gastritis, bile reflux gastritis and gastric ulcer.

The invention relates to a propagating method of Dendrobium officinale Kimura et Migo seedlings, characterized in that, vigorous Dendrobium officinale Kimura et Migo maternal plant is performed with artificial pollination, the obtained fruit is sanitized and cut, seeds are used for bourgeoning culture medium, causing the seeds to bourgeon protocorm and further form young seedling, then the young seedling propagated in s test tube is cultivated by test tube blossom culture medium, the vigorous plant is selected with artificial pollination in the test tube after blossom, cut after fruit ripening with aseptic seeding, causing the seeds to develop into small plant, which is cultivated on good seedling culture medium, mature shoot is formed, the mature shoot is performed with seedling exercising in the natural light, furthermore cultivated into the seedling. The invention is simple in operation, low in cost, greatly shortened in maturing stage of the fruit of Dendrobium officinale Kimura et Migo, high in efficiency and no plant diseases and insect pests, and transplanting success ratio reaches more than 95%.

The invention discloses a hormone-free tissue culture method for dendrobium candidum, which comprises four tissue culture stages including germinating of seeds of the dendrobium candidum into spherical stems, multiplication and differentiation, and rootage and sound seedlings at stem sections; culture media used at the four stages include a hormone-free MS (Murashige and Skoog) culture medium, a hormone-free MS basal culture medium plus 15-20 g/l mashed potato culture medium, a hormone-free MS basal culture medium plus 15-20 g/l mashed banana plus 15-20 g/l mashed potato culture medium and a hormone-free 1/2 MS basal culture medium plus 15-20 g/l mashed banana plus 15-20 g/l mashed potato culture medium. The polysaccharide content and total alkaloid content in the culture of the dendrobium candidum obtained by hormone-free culture are increased compared with hormone-containing culture; the growth state of the dendrobium candidum is normal; the genetic diversity of the plant variety is protected; with the adoption of the method, the tissue culture of the dendrobium candidum is more convenient, green and environmentally friendly, and the resources are saved.

The invention discloses a three-dimensional original ecological cultivation method for dendrobium candidum in forest. The three-dimensional original ecological cultivation method comprises the following steps of: firstly, selecting a cultivation environment; secondly, preparing a cultivation pot; thirdly, paving a matrix in the pot; fourthly, selecting seedlings; fifthly, permanently planting; sixthly, planting in a hanging manner or binding the seedling on the tree; seventhly, managing the cultivation; and eighthly, harvesting. The three-dimensional original ecological cultivation method disclosed by the invention provides a three-dimensional environment of original forest; the healthy growth of the dendrobium candidum is ensured; and the industrial and scale development of the dendrobium candidum can be realized; and the three-dimensional original ecological cultivation method disclosed by the invention has the advantages of high survival rate of seedlings, high yield, high quality of products, low cultivation cost and simpleness for management and operation.

The invention discloses a manufacturing method for extracting and processing fresh juice stock solution by using dendrobium officinale as a raw material. The method furthest protect the color, flavor, nutrition and effective ingredients of dendrobium officinale fresh juice by surface sterilization of the collected fresh dendrobium officinale and technology of juicing, enzymolysis, vacuum degasification, micro-filtration, ultra-filtration, sterile filling, high-temperature instantaneous sterilization and the like, and meanwhile, the product is favorable for storing and realizing industrialized production. The dendrobium officinale is processed into the dendrobium officinale fresh juice stock solution which is easy to store, convenient to carry, simple to administrate, good in mouthfeel and easy to absorb and has health-care nutritional components and medicinal functions; the fresh juice stock solution does not contain any additive; and the content of the fresh juice stock solution reaches over 99 percent. The dendrobium officinale fresh juice stock solution can be administrated separately, also can be added into mineral water to form health-care beverage, also can be dissolved into wine to form nourishing health-care wine, and can be added into high-grade dishes to form nourishing dishes for eating.

The invention provides a preparation method of dendrobium nobile polysaccharide extracts, which is realized by steps of degreasing, extracting, alcohol precipitating, protein removing, monose and oligosaccharide removing and purifying gel columns and the like. The polysaccharide content in the polysaccharide extract is more than 93 percent. The polysaccharide extract has remarkable oxidation prevention activity, can be combined with medicament excipients and carriers permitted by preparation agents, and can be applied in preparing medicaments or medicament combinations for treating and preventing cardiovascular diseases. The preparation method of the dendrobium nobile polysaccharide extracts is reasonable in design and convenient in operation.

The invention provides a processing method capable of reserving all components of dendrobium candidum. The processing method comprises the following steps: (1) preliminarily processing the medicinal material; (2) freeze-drying in vacuum; (3) preparing common powder; (4) preparing ultrafine powder; and (5) preparing ultrafine particles, wherein the step of freeze-drying in vacuum comprises the following three processes: pre-freezing; sublimation drying; and desorption drying. The product produced by the method has the advantages of fresh color, aromatic flavor and fully reserved nutrient components without loss, and is convenient to serve; meanwhile, the product is thoroughly dewatered, thus being convenient for long-term preservation, reprocessing and transportation at normal temperature.

The invention discloses a method for cultivating algam Dendrobium nobile in group in water. Since the blades of algam Dendrobium nobile before output bottle will not form complete horny layer to process effective photosynthesis, according to the growth and physiological characters, the algam Dendrobium nobile output bottle is cultivated by water cultivate device, to be cultivated for 30 days at right condition, therefore, cultivated seeds can be transplanted into large field. The invention can effectively resolve the problems as low survival rate and improve survival rate to 95%.

The invention discloses a method for extracting various active ingredients from dendrobium officinale. The method comprises the following steps of: carrying out flash-extracting; centrifuging the flash-extracted crude extracting solution; carrying out alcohol precipitation and tertiary dehydration to the centrifuged liquid supernatant to obtain free polysaccharide; combining the centrifuged precipitates with polysaccharide and alkaloid extract; and extracting the various active ingredients from the dendrobium officinale, so that the comprehensive utilization of the dendrobium officinale resources is realized. The method disclosed by the invention is high in yield, capable of greatly shortening the extraction time during the extraction process, capable of avoiding the influences of thermal destruction to the products, capable of reducing the production cost and applicable to large-scale production.

The invention relates to a light control method for dendrobium officinale tissue culture, which comprises the following steps of: obtaining powdery seeds after dendrobium officinale fruits are sterilized, culturing the powdery seeds through a seed germination culture medium, and germinating the cultured seeds to form protocorms. The method is characterized in that: in the tissue culture process of dendrobium officinale seedlings, a light emitting diode (LED) plant growth illumination system is used as a light source, and different illumination treatment is implemented according to different stages of dendrobium officinale tissue culture. The different illumination treatment is implemented according to different growth stages so that the dendrobium officinale seedlings can furthest utilize light energy for photosynthesis and the highest growth amount is obtained. Experimental results show that: the culture period of the dendrobium officinale seedlings can be effectively shortened according to the technical scheme, the time from the seeds to the rooted finished seedlings is only 150 to 180 days, the growth period is shortened by 30 to 60 days compared with the culture under a common fluorescent lamp, and the seedlings cultured by the method have stable hereditary character and good quality.

The invention provides a traditional Chinese medicinal dendrobium officinale preparation and a preparation method thereof. In the preparation method, by adopting freeze-drying and ultra-fine pulverization methods, dendrobium officinale is frozen to a brittle point or below vitreous temperature so that the dendrobium officinale is in a brittle state, then the dendrobium officinale is dried and pulverized in mechanical and pneumatic modes so that cell walls are broken, and finally ultra-fine powder is obtained. The preparation method has the beneficial effects of increasing specific surface area of the preparation, improving dissolution rate and bioavailability of effective ingredients, increasing absorption rate and absorption capacity for human body, enhancing pharmacological action and improving medicament effect up to 3-10 times as that of the traditional dendrobium officinale decoction pieces, thus reducing medicament consumption and saving medicinal materials.

The invention relates to a planting method of famous and precious Chinese herb medicine dendrobium officinale, and the method is characterized in that a cultivation bed of a plantation trough with an undulated bottom is established under a certain facility environment, multiple mixed organic matrixes mainly comprising pine bark are paved, a nondestructive planting technology for stretching tissue culture seedling roots is adopted, and scientific and reasonable dendrobium officinale planting method adopting comprehensive cultivation measures such as a photo and temperature control technology, a fertilizer control technology, an insect and harmful organism nuisanceless control technology and a sustainable harvesting technology is adopted in different growth seasons and for different plants according to the biological characteristics of the dendrobium officinale; and due to the adoption of the method, the ventilation, moisture evaporation and moistening functions of a plant root environment can be enhanced, and harmful substances influencing the growth of the plant can be eliminated in time, so that the plant can grow strong, active components are highly concentrated, the resistance is enhanced, a purpose of high yield and excellent quality can be realized, the high yield and stable yield of the dendrobium officinale can be guaranteed, the product quality is excellent, sustainability for harvesting the dendrobium officinale for multiple years can be realized, and super high yield of fresh products about 600kg per Mu every year can be realized after the method is put into production.

The invention discloses a vacuum freeze-drying processing method of dendrobium officinale, comprising the following steps of: (1) washing and draining fresh steam sections of dendrobium officinale and cutting into segments; (2) arranging the drained dendrobium officinale in a freeze-drying device for pre-freezing and constant-temperature freezing at 30 DEG C below zero to 35 DEG C below zero; (3)carrying out sublimation drying on the dendrobium officinale processed in the step (2); (4) heating the dendrobium officinale after the sublimation drying in the step for (3) for evaporating residualwater; and (5) packing the condensed dendrobium officinale products with water content of 6-9% dried in the step (4) into clean and sanitary food-grade aluminum-foil bags, sealing to the dendrobium officinale products. The dendrobium officinale products processed by the method disclosed by the invention are rich in dendrobium officinale polysaccharide, and can be directly eaten or taken as ingredients for stewing soup to eat or making tea to drink. The dendrobium officinale products keep original state, taste and pharmacological effects of the dendrobium officinale, can be stored and transported at normal temperature, and has more than five years of shelf life.

The invention belongs to the biotechnology field and relates to a hormone-free cultivation and rapid propagation method of dendrobium candidum axenic seedlings. The hormone-free cultivation and rapid propagation method comprises the steps: germinating dendrobium candidum seeds in an axenic mode; forming and preserving protocorms and seedlings; rooting and proliferating unrooted seedlings, cultivating sound seedlings; and toughening and transplanting the axenic seedlings. The technology has convenient operation, small occupied space and low production cost, is convenient to industrially produce and apply on a large scale and is beneficial to retaining the excellent property of the seedlings and increase the use safety of the seedlings when being used for propagating the dendrobium candidum.

The invention relates to a method for cultivating algam Dendrobium nobile, wherein it comprises that putting the seed of algam Dendrobium nobile into 1/2MS basic medium with NNA (a- fruitone), banana juice and agar; then transplanting the former corm into 1/2MS basic medium with BA (6- benzyl adenine), banana juice and agar, to cultivate small seed into middle and large seeds; then planting the large seed into the hole of wall.The invention can improve the yield and confirm the better penetration and enough light of large seed.

The invention discloses a method for brewing dendrobium nobile wine. Dendrobium nobile is used as one of raw materials, corns and potatoes are used as matrixes, and the dendrobium nobile wine is prepared by the procedures of pretreating, mixing, medicine feeding, primary fermenting, secondary fermenting, squeezing, clarifying, wine decocting, inspecting, bottling and storing. During production, the dendrobium nobile serving as the raw material is subjected to combined hydrolysis by using cellulose and pectinase, so that nutrients beneficial to a human body, contained in the Dendrobium nobile,can be separated out fully; and meanwhile, the corns and the potatoes are used as the matrixes, so that the production cost is reduced and simultaneously the prepared dendrobium nobile wine is red brown, has a unique scorch flavor, is mellow, palatable and slightly bitter and has a long-lasting aftertaste. A product of the dendrobium nobile wine has multiple obvious health-care functions of removing lead, enhancing memory ability and immunity, deferring senility, increasing anoxia resistance, preventing osteoporosis and the like. The method is easy to master and can be applied to scale production.

The invention discloses an artificial light cultivating method of dendrobium candidum, belonging to the technical field of medical plant cultivation. The method comprises the following steps: naturalized young plants are transplanted to a nutrition pot or a potted tray containing base material and placed in a three-dimensional cultivating shelf of an artificial light closed plant factory; wherein cultivating conditions are as follows: the illumination intensity is 60-150 mu mol.m-2.s-1; the illumination time is 9-12h.d-1, the temperature is 24-27 DEG C; the relative humidity is 65-80%; the CO2 concentration is 400-800 mu mol.m-1; and the air velocity is 0.3-0.6 m.s-1; after the young plants are transplanted, atomizing keeps the humidifying state of leaf surfaces of the young plants before 2 weeks; after two weeks, the young plants are watered; according to the principle of keeping the humidification of the base material, macroelement nutrient solution is applied every 7 to 15 days; microelement nutrient solution is applied every 25 to 35 days; bottom surface is watered every 3 to 5 days; the young plants are harvested after being cultivated for 1.5 to 2 years and every half year is a picking time. The method shortens the production period, guarantees the uniform quality of the products, increases the yield, and is easy to realize standardization, industrialization and scale production.

A method for directly seedling dendrobe seedlings by using a plastic greenhouse comprises the following steps: constructing a greenhouse; making a seedling bed; preparing a base material and performing sterilization, which can be realized by mixing pine barks and sheep manure, filtering the pine barks and the sheep manure through a screen after completely fermenting the pine barks and the sheep manure, soaking the pine barks and the sheep manure by using carbendazim, spreading the pine barks and the sheep manure on the seedling bed, placing the pine barks and the sheep manure on the seedling bed for 5-10 days, and controlling the water content; inoculating the symbiotic bacteria, which can be realized by taking a two years old base material of the dendrobe and uniformly scattering the two years old substrate on the seedling bed on which a new substrate is laid; pre-treating seeds, which can be realized by completely moisturizing the seeds by using sterile water containing naphthyl acetic acid; sterilizing the seeds in the greenhouse, which can be realized by fumigating every cubic meter of seeds by using 4 g mushroom protector, fumigating the seeds for one time before sowing, and fumigating the seeds every half a month in the future; scattering, which can be realized by after the seeds are scattered and before the seedlings are transplanted, controlling temperature in the greenhouse, water content of the seedling bed and air humidity, treating the seeds by weak lights within the first month of the scattering, and gradually enhancing illumination after budding; managing water, fertilizers and diseases of the seedlings; and transplanting the seedlings. The method for directly seedling the dendrobe seedlings by using the plastic greenhouse, provided by the invention has the advantages that as the dendrobe are transplanted after directly becoming to the seedlings, the seedling breeding cost is effectively reduced, the seedling rate of the seeds is more than 90 percent, and the transplanting survival rate of the seedlings is 100 percent.