The invention relates to the technical field of
tissue culture, and particularly discloses a
tissue culture and rapid propagation method of a new variety of
Dendrobium nobile, which comprises the following steps: S1, selecting current-year new lateral buds, removing leaf sheaths and retaining
bud points, and sequentially washing with running water, soaking with 75%
alcohol, disinfecting with 0.1%
mercury bichloride and washing with
sterile water; s2, the disinfected stem segments with buds are inoculated into an induction culture medium, the induction culture medium is a 1 / 2 MS culture medium, and 3.0 mg / L of 6-BA, 0.5 mg / L of NAA and 60 g / L of potato
powder are added; s3, adventitious buds generated through induction are
cut off and inoculated into a proliferation culture medium, the proliferation culture medium is a 1 / 2 MS culture medium, and 3.0 mg / L of 6-BA, 0.5 mg / L of NAA and 80 g / L of potato
powder are added; s4, the proliferated adventitious buds are inoculated into a rooting culture medium, the rooting culture medium is a 1 / 2 MS culture medium, and 0.5 mg / L of NAA or 0.5 mg / L of IBA is added; according to the method, the culture medium formula and the culture process are optimized, so that the
tissue culture efficiency of the
Dendrobium nobile 'Hooke Chaoya' is remarkably improved.