Preparation method of plant composition

Abstract

The invention relates to the technical field of plant compositions, in particular to a preparation method of a plant composition, which is simple, convenient and reasonable in process, and the obtained plant extract has obvious effects of delaying skin aging and improving body functions, can promote the generation of ATP in fibroblasts, and has a good application prospect. The traditional Chinese medicine composition can inhibit the gene expression of metalloproteinase-1 and metalloproteinase-3 in senescence fibroblasts, can achieve the effects of improving the body function and resisting the senescence of organs such as the skin through oral administration or skin administration, and is rich in resource because the astragalus membranaceus and the dendrobium nobile are mainly adopted as the raw materials; the extracted plant composition extract has a remarkable anti-aging effect, and the plant composition which is rich in resource and remarkable in effect can be obtained, so that the problems that an existing plant composition is high in price and not remarkable in effect are solved.

Description

Technical Field

[0001] The present invention relates to the technical field of plant compositions, and in particular to a method for preparing the plant composition. Background Art

[0002] Aging refers to the natural phenomenon that after an organism matures under normal conditions, its own tissue structure gradually undergoes degenerative changes with age, the function of the body's organs declines, the ability of the internal environment to stabilize itself weakens, and the resistance to internal and external environmental damage factors decreases, leading to death. Studies have found that the production of ATP by fibroblasts decreases during the aging process, so the reduction of ATP indicates the decline of the body's skills. At the same time, during the aging process, the expression of extracellular matrix metalloproteinase-1 and extracellular matrix metalloproteinase-3 increases, causing the degradation of different types of collagen and other proteins, resulting in aging phenomena such as deepening wrinkles and sagging skin.

[0003] At present, skin care products containing plant active ingredients are favored by consumers because of their mild effects, few side effects, and easily absorbed components compared to traditional cosmetics.

[0004] Based on the research methods of modern molecular biology, people are constantly developing various preparations to inhibit skin aging, among which Chinese medicine based on TCM theory is one of the research hotspots. Studies have found that the effect of Chinese herbal medicines in delaying aging has the following problems to be solved: herbal medicines with significant effects often have the problem of scarce resources; herbal medicines with abundant resources are not effective enough. Summary of the invention

[0005] The purpose of the present invention is to provide a method for preparing a plant composition, aiming to solve the problem that the existing plant composition is expensive and has insignificant effect.

[0006] To achieve the above object, the present invention provides a method for preparing a plant composition, comprising the following steps:

[0007] The raw materials of astragalus and dendrobium are transformed by microorganisms using ganoderma lucidum to obtain microbial transformation substances;

[0008] extracting the microbial conversion substance with an aqueous alcohol solution to obtain a plant extract;

[0009] The plant extract is allowed to stand at a low temperature of 0°C to 10°C for 3 to 24 hours, then extracted with a solvent, filtered and purified to obtain an extract;

[0010] Passing the extract through a molecular interception device to intercept the throttling liquid less than 5000 Daltons, and concentrating it into an extract;

[0011] One or more of glycerol, propylene glycol, butylene glycol and 1,2-hexanediol are added to the extract, and lactic acid is added to adjust the pH value to 3-8, stir and fill to obtain a liquid finished product of the plant composition.

[0012] Among them, "using Ganoderma lucidum to transform raw materials Astragalus and Dendrobium to obtain microbial transformation substances" includes:

[0013] Take 1.0L of potato extract, 20.0g of glucose, 3.0g of KH2PO4, 1.5g of MgSO4·7H2O, a trace amount of vitamin B1 and 15.0g of agar at pH 6.0, sterilize at 126℃ for 30 minutes, and cool to prepare a liquid culture medium;

[0014] Inoculating 1% of the Ganoderma lucidum strains on the liquid culture medium, and fermenting and culturing at a temperature of 15° C. to 40° C. for 3 to 15 days to obtain Ganoderma lucidum liquid strains;

[0015] A preset amount of astragalus and a preset amount of dendrobium are mixed together, and after the mixed powder is crushed into a particle size of 20 mesh to 60 mesh, 1.5 to 2.5 times of water in terms of the total mass of astragalus and dendrobium are added, and after sterilization, 2% to 12% of the total mass of astragalus and dendrobium liquid strains are inoculated, and fermented and cultured at a temperature of 15° C. to 40° C. for 5 to 18 days, and then high-temperature inactivation is performed to obtain microbial transformation substances.

[0016] Among them, in “1.0L of potato extract, 20.0g of glucose, 3.0g of KH2PO4, 1.5g of MgSO4·7H2O, a trace amount of vitamin B1 and 15.0g of agar with pH 6.0, sterilize at 126°C for 30 minutes, and cool to obtain a liquid culture medium;”, the preparation steps of the potato extract are:

[0017] Take 200 g of peeled potatoes, cut them into small pieces, add 1.0 L of water and boil for 30 minutes; filter the potato pieces to obtain a potato extract.

[0018] Among them, in "mixing a preset amount of astragalus and a preset amount of dendrobium together, crushing the mixed particles into a particle size of 20-60 mesh, adding water 1.5-2.5 times the total mass of astragalus and dendrobium, sterilizing, inoculating 2%-12% of the total mass of astragalus and dendrobium liquid culture, fermenting and culturing at a temperature of 15°C-40°C for 5-18 days, and then inactivating at high temperature to obtain microbial transformation material.", the preset amount of astragalus is 60%-80%, and the preset amount of dendrobium is 20%-40% of the astragalus.

[0019] Among them, the process of “standing the plant extract at a low temperature of 0°C to 10°C for 3 to 24 hours, extracting with a solvent, filtering and purifying to obtain an extract” includes:

[0020] Placing the plant extract at a temperature of 0°C to 10°C for 3 to 24 hours;

[0021] According to the polarity of the target compound, choose a suitable solvent for extraction;

[0022] Mixing the extract after standing at low temperature with a selected solvent in a preset ratio to obtain a mixed solution;

[0023] Performing preliminary filtration on the mixed solution using a filter material to obtain a filtrate;

[0024] The filtrate is eluted using an adsorption column and an eluent system to obtain an extract.

[0025] The preparation method of a plant composition of the present invention comprises the following steps: subjecting raw materials, astragalus and dendrobium, to microbial transformation using ganoderma lucidum to obtain microbial transformation substances; extracting the microbial transformation substances using an alcohol aqueous solution to obtain a plant extract; allowing the plant extract to stand at a low temperature of 0°C to 10°C for 3 to 24 hours, then extracting with a solvent, filtering and purifying to obtain an extract; subjecting the extract to a molecular interception device to intercept a throttling liquid less than 5000 Daltons, and concentrating the extract to an extract; adding one or more of glycerol, propylene glycol, butylene glycol, and 1,2-hexanediol to the extract, and adding lactic acid, adjusting the pH value to 3 to 8, stirring and filling to obtain a liquid finished product of the plant composition. The invention is simple and convenient, with reasonable process. The obtained plant extract has obvious effects of delaying skin aging and improving body functions, can promote the production of ATP in fibroblasts, and inhibit the gene expression of metalloproteinase-1 and metalloproteinase-3 in aged fibroblasts. It can be administered orally or by skin administration to exert the effects of improving body functions and resisting aging of organs such as skin. Astragalus and dendrobium are mainly used as raw materials, which are rich in resources, and the extracted plant combination extract has a more significant anti-aging effect, a plant composition with rich resources and significant effects can be obtained, thereby solving the problems of the existing plant combination being expensive and having insignificant effects. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0027] Figure 1 The present invention is a flow chart of a method for preparing a plant composition.

[0028] Figure 2 The present invention is a flow chart of microbial transformation of raw materials, astragalus and dendrobium, by using ganoderma lucidum to obtain microbial transformation substances.

[0029] Figure 3 The invention is a flow chart of placing the plant extract at a low temperature of 0°C to 10°C for 3 to 24 hours, extracting with a solvent, filtering and purifying to obtain an extract. DETAILED DESCRIPTION

[0030] Embodiments of the present invention are described in detail below, examples of which are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are intended to be used to explain the present invention, and should not be construed as limiting the present invention.

[0031] See also Figures 1 to 3 The present invention provides a method for preparing a plant composition, comprising the following steps:

[0032] S1: using Ganoderma lucidum to transform raw materials of astragalus and dendrobium to obtain microbial transformation substances;

[0033] S11: 1.0 L of potato extract, 20.0 g of glucose, 3.0 g of KH2PO4, 1.5 g of MgSO4·7H2O, a trace amount of vitamin B1 and 15.0 g of agar at pH 6.0 were sterilized at 126°C for 30 minutes and cooled to obtain a liquid culture medium;

[0034] Take 200 g of peeled potatoes, cut them into small pieces, add 1.0 L of water and boil for 30 minutes; filter the potato pieces to obtain a potato extract.

[0035] Specifically, microbial transformation refers to the structural modification of complex substrates by microbial cells, that is, the catalytic reaction of specific parts of the substrate using one or a series of enzymes produced during microbial metabolism.

[0036] S12 inoculating 1% of the Ganoderma lucidum strain on the liquid culture medium, and fermenting and culturing at a temperature of 15° C. to 40° C. for 3 to 15 days to obtain a liquid Ganoderma lucidum strain;

[0037] Specifically, the Ganoderma lucidum strain is the Ganoderma lucidum strain stored in the China Forestry Microorganism Culture Collection Management Center, and the Ganoderma lucidum liquid strain is preferably fermented and cultured at 23° C. to 28° C. for 5 to 8 days.

[0038] S13: Mix a preset amount of astragalus and a preset amount of dendrobium together, grind the mixed particles into a particle size of 20-60 mesh, add water of 1.5-2.5 times the total mass of astragalus and dendrobium, sterilize, inoculate 2%-12% of the total mass of astragalus and dendrobium in liquid form, ferment and culture at a temperature of 15°C-40°C for 5-18 days, and then perform high-temperature inactivation to obtain microbial transformation substances.

[0039] The preset amount of the astragalus is 60% to 80%, and the preset amount of the dendrobium is 20% to 40% of the astragalus.

[0040] Specifically, the liquid spawn of Ganoderma lucidum is inoculated into a solid culture medium composed of Astragalus and Dendrobium for solid fermentation culture; the particle size of Astragalus and Dendrobium is preferably 30-40 mesh; the mass of the inoculated liquid spawn of Ganoderma lucidum is preferably 5% of the total mass of Astragalus and Dendrobium; the solid fermentation culture is preferably cultured at 23°C-28°C for 8-12 days.

[0041] S2 extracting the microbial transformation substance with an alcohol aqueous solution to obtain a plant extract;

[0042] Specifically, the concentration of the alcohol aqueous solution is 10% to 75% by mass, preferably 30%, the mass of the alcohol aqueous solution is 8 to 20 times the mass of the microbial transformation material, preferably 10 to 15 times, the extraction temperature is 40°C to 95°C, preferably 65°C to 85°C, the number of extractions is 1 to 3 times, preferably 2 times.

[0043] S3: placing the plant extract at a low temperature of 0°C to 10°C for 3 to 24 hours, extracting with a solvent, filtering and purifying to obtain an extract;

[0044] S31 placing the plant extract at a temperature of 0° C. to 10° C. for 3 to 24 hours;

[0045] Specifically, the liquid spawn of Ganoderma lucidum is inoculated into a solid culture medium composed of Astragalus and Dendrobium for solid fermentation culture; the particle size of Astragalus and Dendrobium is preferably 30-40 mesh; the mass of the inoculated liquid spawn of Ganoderma lucidum is preferably 5% of the total mass of Astragalus and Dendrobium; the solid fermentation culture is preferably cultured at 23°C-28°C for 8-12 days.

[0046] S32 selects a suitable solvent for extraction according to the polarity of the target compound;

[0047] Specifically, according to the polarity of the target compound, a suitable solvent is selected for extraction, such as water, ethanol, ethyl acetate, etc.

[0048] S33: mixing the extract after standing at low temperature with a selected solvent in a preset ratio to obtain a mixed solution;

[0049] Specifically, the extract after standing at low temperature is mixed with a selected solvent in a certain proportion, and the extraction efficiency can be increased by stirring, oscillating or ultrasonic-assisted methods.

[0050] S34 uses a filter material to preliminarily filter the mixed solution to obtain a filtrate;

[0051] Specifically, the filter material includes any one of gauze, filter paper or filter cloth, and the mixed liquid is preliminarily filtered using the filter material to obtain a filtrate.

[0052] S35 uses an adsorption column and an eluent system to elute the filtrate to obtain an extract.

[0053] Specifically, an appropriate adsorption column and eluent system are selected so that the impurities are eluted first and the target compound flows out later.

[0054] S4: passing the extract through a molecular interception device to intercept a throttling liquid with a molecular weight less than 5000 Daltons and concentrating the extract into an extract;

[0055] Specifically, the optimal temperature for low-temperature standing is 1° C. to 4° C., the optimal low-temperature standing time is 10 to 18 hours, and the best concentration is 1.5 to 2.5 times the mass of the raw material.

[0056] S5: adding one or more of glycerol, propylene glycol, butylene glycol, 1,2-hexanediol, and lactic acid to the extract, adjusting the pH value to 3-8, stirring and filling to obtain a liquid finished product of the plant composition.

[0057] Specifically, the optimal amount of glycerol is 10% to 30% of the mass of the concentrated plant extract, the optimal amount of propylene glycol is 10% to 20% of the mass of the concentrated plant extract, the pH value is optimally adjusted to 3.5 to 6.5, in addition to lactic acid, citric acid and hydrochloric acid can also be used to adjust the pH value, and the addition amount of 1,2-hexanediol is about 5%, which will have a clear preservative effect and can be used to replace preservatives. After mixing, stir evenly and fill to obtain a liquid product.

[0058] Example 1

[0059] (1) Take 200 g of peeled potatoes, cut them into small pieces, add 1.0 L of water and boil for 30 min; filter the potato pieces to obtain a potato extract.

[0060] (2) 1.0 L of potato extract, 20.0 g of glucose, 3.0 g of KH2PO4, 1.5 g of MgSO4·7H2O, a trace amount of vitamin B1 and 15.0 g of agar at pH 6.0 were sterilized at 126°C for 30 minutes and cooled to obtain a liquid culture medium;

[0061] (3) inoculating 1% of the Ganoderma lucidum strain on the liquid culture medium, and fermenting and culturing at a temperature of 25° C. for 7 days to obtain a liquid strain of Ganoderma lucidum;

[0062] (4) 70 g of Astragalus and 20 g of Dendrobium were mixed together, and the mixture was crushed into a particle size of 30 mesh. Then, 150 g of water was added, and after sterilization, 4.5 g of liquid spawn of Ganoderma lucidum was inoculated. The mixture was fermented and cultured at 25° C. for 10 days, and then inactivated at high temperature to obtain microbial transformation substances.

[0063] (5) the microbial transformation material was allowed to stand at 1° C. for 10 hours, stirred evenly with 10 times 70° ethanol, and extracted twice with stirring at 70° C. to obtain a plant extract;

[0064] (6) using gauze to preliminarily filter the mixed solution to obtain a filtrate;

[0065] (7) Using an adsorption column and an eluent system to elute the filtrate to obtain an extract.

[0066] (8) passing the extract through a molecular interception device to intercept the throttling liquid with a mass less than 5000 Daltons, and concentrating the extract to 1.5 times the mass of the raw material;

[0067] (9) Add 10% of the mass of the plant extract to the extract, add lactic acid, adjust the pH value to 3.5, add 5% of the total amount of 1,2-hexanediol, stir well and fill to obtain a liquid finished product of the plant composition.

[0068] Example 2

[0069] (1) Take 200 g of peeled potatoes, cut them into small pieces, add 1.0 L of water and boil for 30 min; filter the potato pieces to obtain a potato extract.

[0070] (2) 1.0 L of potato extract, 20.0 g of glucose, 3.0 g of KH2PO4, 1.5 g of MgSO4·7H2O, a trace amount of vitamin B1 and 15.0 g of agar at pH 6.0 were sterilized at 126°C for 30 minutes and cooled to obtain a liquid culture medium;

[0071] (3) inoculating 1% of the Ganoderma lucidum strain on the liquid culture medium, and fermenting and culturing at a temperature of 15° C. for 3 days to obtain a liquid Ganoderma lucidum strain;

[0072] (4) 70 g of Astragalus and 28 g of Dendrobium were mixed together, and the mixture was crushed into a particle size of 20 mesh. Then, 196 g of water was added, and after sterilization, 1.96 g of liquid spawn of Ganoderma lucidum was inoculated. The mixture was fermented and cultured at a temperature of 15° C. for 5 days, and then inactivated at high temperature to obtain microbial transformation substances.

[0073] (5) the microbial transformation material was allowed to stand at 3° C. for 15 hours, stirred evenly with 12 times 25° ethanol, and extracted once by shaking at 40° C. to obtain a plant extract;

[0074] (6) using filter paper to preliminarily filter the mixed solution to obtain a filtrate;

[0075] (7) Using an adsorption column and an eluent system to elute the filtrate to obtain an extract.

[0076] (8) passing the extract through a molecular interception device to intercept the throttling liquid with a mass less than 5000 Daltons and concentrating it to twice the mass of the raw material;

[0077] (9) Add propylene glycol in an amount of 10% by weight of the plant extract to the extract, add lactic acid, adjust the pH value to 5, add 1,2-hexanediol in an amount of 5% by weight, stir well, and fill to obtain a liquid finished product of the plant composition.

[0078] Example 3

[0079] (1) Take 200 g of peeled potatoes, cut them into small pieces, add 1.0 L of water and boil for 30 min; filter the potato pieces to obtain a potato extract.

[0080] (2) 1.0 L of potato extract, 20.0 g of glucose, 3.0 g of KH2PO4, 1.5 g of MgSO4·7H2O, a trace amount of vitamin B1 and 15.0 g of agar at pH 6.0 were sterilized at 126°C for 30 minutes and cooled to obtain a liquid culture medium;

[0081] (3) inoculating 1% of the Ganoderma lucidum strain on the liquid culture medium, and fermenting and culturing at a temperature of 40° C. for 15 days to obtain a liquid strain of Ganoderma lucidum;

[0082] (4) 80 g of Astragalus and 20 g of Dendrobium were mixed together, and the mixture was crushed into a particle size of 60 mesh. Then, 250 g of water was added, and after sterilization, 12 g of liquid spawn of Ganoderma lucidum was inoculated. The mixture was fermented and cultured at 40° C. for 18 days, and then inactivated at high temperature to obtain microbial transformation substances.

[0083] (5) the microbial transformation material was allowed to stand at 4° C. for 18 hours, stirred evenly with 15 times 50% ethanol, and then ultrasonically extracted three times at 80° C. to obtain a plant extract;

[0084] (6) performing preliminary filtering on the mixed solution using a filter cloth to obtain a filtrate;

[0085] (7) Using an adsorption column and an eluent system to elute the filtrate to obtain an extract.

[0086] (8) passing the extract through a molecular interception device to intercept the throttling liquid with a mass less than 5000 Daltons and concentrating it to 2.5 times the mass of the raw material;

[0087] (9) Add propylene glycol in an amount of 10% by weight of the plant extract to the extract, add citric acid, adjust the pH value to 8, add 1,2-hexanediol in an amount of 5% by weight, stir well and fill to obtain a liquid finished product of the plant composition.

[0088] Effect Example

[0089] 1. Experimental materials: DMEM high glucose medium (Thermo Fisherscientific), fetal bovine serum (Thermo Fisherscientific), trypsin-EDTA (Thermo Fisherscientific), H2O2 (Sigma), PrestoBlue kit (Thermo Fisherscientific), ATP detection kit (Biyuntian), HFF fibroblasts (Stem Cell Bank, Shanghai Cell Bank, Chinese Academy of Sciences), etc.

[0090] 2. Experimental methods

[0091] 2.1 Cytotoxicity assay

[0092] Take the HFF fibroblast suspension in the logarithmic phase and grow well, and inoculate it into a 96-well plate containing 10% fetal bovine serum and 1% double-antibody DMEM medium, with 2-3×10 cells per well. 4 After 24 hours of incubation, different concentrations of samples were added, and a control group (wells without samples and cells), blank wells (wells without samples and cells) and sample blank wells (wells with samples and cells) were set up. Three replicate wells were set up for each concentration. After 24 hours of growth, the fluorescence absorbance value was detected using the Presto Blue method to calculate the cell survival rate.

[0093] Cell viability (%) = (sample OD value - sample blank group OD value) / (control group OD value - blank group OD value).

[0094] 2.2 Effects on APT of HFF fibroblasts

[0095] HFF fibroblasts were seeded in 48-well culture plates with 3 replicate wells in each group. After 24 hours of culture, the samples were applied to HFF fibroblasts. After 24 hours, the culture medium was discarded from each well, and fresh culture medium was added. 300 μM H2O2 was added to stimulate the experimental group cells. After 2 hours of action, the cells were lysed for ATP detection.

[0096] 2.3 Statistical analysis of experimental data

[0097] All data were expressed as mean ± standard deviation. The groups were compared using t-test. P < 0.05 was considered to be significantly different, and P < 0.01 was considered to be extremely significantly different.

[0098] 3. Experimental results

[0099] 3.1 Cytotoxicity test results

[0100] Table 1 Toxicity results of each sample on HFF fibroblasts

[0101]

[0102]

[0103] According to the results in Table 1, the concentrations of 0.01% to 5% of Examples 1 to 3 were not cytotoxic to HFF fibroblasts, and 10% of Examples 1 to 3 had certain cytotoxicity. In the later experiments, samples with a concentration of 0.5% to 5% were selected for testing.

[0104] 3.2 Effects of samples on APT of HFF fibroblasts

[0105] Table 2 ATP concentration detection of H2O2-induced fibroblast senescence cell model treated with samples of different concentrations

[0106]

[0107] Note: The significance analysis between the model group and the blank group was #p<0.05; the significance analysis between the tested samples and the model group was *p<0.05, **p<0.01.

[0108] According to the results in Table 2, there is a difference in ATP content between the model group and the blank group, indicating that the modeling is successful. At the same time, according to Table 2, the ATP content of Examples 1 to 3 at a concentration of 0.05% is equivalent to that of the model group, while the ATP content of Examples 1 to 3 at concentrations of 0.1% and 0.2% is significantly higher than that of the model group and the blank group, and the ATP content of Example 1 is slightly higher than that of Examples 2 and 3.

[0109] The above disclosure is only a preferred embodiment of the method for preparing a plant composition of the present invention, and it is certainly not used to limit the scope of the present invention. A person skilled in the art can understand that all or part of the processes of the above embodiment and equivalent changes made according to the claims of the present invention still fall within the scope of the invention.

Claims

1. A method for preparing a plant composition, characterized in that: The following steps are involved: The raw materials of astragalus and dendrobium are transformed by microorganisms using ganoderma lucidum to obtain microbial transformation substances; extracting the microbial conversion substance with an aqueous alcohol solution to obtain a plant extract; The plant extract is allowed to stand at a low temperature of 0°C to 10°C for 3 to 24 hours, then extracted with a solvent, filtered and purified to obtain an extract; Passing the extract through a molecular interception device to intercept the throttling liquid less than 5000 Daltons, and concentrating it into an extract; One or more of glycerol, propylene glycol, butylene glycol and 1,2-hexanediol are added to the extract, and lactic acid is added to adjust the pH value to 3-8, stir and fill to obtain a liquid finished product of the plant composition.

2. The method for preparing the plant composition according to claim 1, characterized in that: In "subjecting raw materials of Astragalus and Dendrobium to microbial transformation using Ganoderma lucidum to obtain microbial transformation substances", it includes: Take 1.0L of potato extract, 20.0g of glucose, 3.0g of KH2PO4, 1.5g of MgSO4·7H2O, a trace amount of vitamin B1 and 15.0g of agar at pH 6.0, sterilize at 126℃ for 30 minutes, and cool to prepare a liquid culture medium; Inoculating 1% of the Ganoderma lucidum strains on the liquid culture medium, and fermenting and culturing at a temperature of 15° C. to 40° C. for 3 to 15 days to obtain Ganoderma lucidum liquid strains; A preset amount of astragalus and a preset amount of dendrobium are mixed together, and after the mixed powder is crushed into a particle size of 20 mesh to 60 mesh, 1.5 to 2.5 times of water in terms of the total mass of astragalus and dendrobium are added, and after sterilization, 2% to 12% of the total mass of astragalus and dendrobium liquid strains are inoculated, and fermented and cultured at a temperature of 15° C. to 40° C. for 5 to 18 days, and then high-temperature inactivation is performed to obtain microbial transformation substances.

3. The method for preparing the plant composition according to claim 2, characterized in that: In "1.0 L of potato extract, 20.0 g of glucose, 3.0 g of KH2PO4, 1.5 g of MgSO4·7H2O, a trace amount of vitamin B1 and 15.0 g of agar at pH 6.0, sterilize at 126° C. for 30 minutes, and cool to obtain a liquid culture medium;", the preparation steps of the potato extract are: Take 200 g of peeled potatoes, cut them into small pieces, add 1.0 L of water and boil for 30 minutes; filter the potato pieces to obtain a potato extract.

4. The method for preparing the plant composition according to claim 3, characterized in that: In the method of "mixing a preset amount of astragalus and a preset amount of dendrobium together, crushing the mixed particles into a particle size of 20-60 mesh, adding water of 1.5-2.5 times the total mass of astragalus and dendrobium, sterilizing, inoculating 2%-12% of the total mass of astragalus and dendrobium in the liquid form of Ganoderma lucidum, fermenting and culturing at a temperature of 15°C-40°C for 5-18 days, and then inactivating the mixture at high temperature to obtain microbial transformation material", the preset amount of astragalus is 60%-80%, and the preset amount of dendrobium is 20%-40% of the astragalus.

5. The method for preparing the plant composition according to claim 4, characterized in that: The step of “standing the plant extract at a low temperature of 0°C to 10°C for 3 to 24 hours, extracting with a solvent, filtering and purifying to obtain an extract” includes: Placing the plant extract at a temperature of 0°C to 10°C for 3 to 24 hours; According to the polarity of the target compound, choose a suitable solvent for extraction; Mixing the extract after standing at low temperature with a selected solvent in a preset ratio to obtain a mixed solution; Performing preliminary filtration on the mixed solution using a filter material to obtain a filtrate; The filtrate is eluted using an adsorption column and an eluent system to obtain an extract.

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