50 results about "Inorganic selenium" patented technology

The invention provides bacillus megaterium and application thereof in preparation of a nano-selenium organic fertilizer and improvement of the selenium content of tea leaves, and belongs to the technical field of microorganisms, the bacillus megaterium NT2 is preserved in the China Center for Type Culture Collection with the preservation number of CCTCC NO: M 20251806, and the bacillus megaterium NT2 is preserved in the China Center for Type Culture Collection with the preservation number of CCTCC NO: M 20251806. The bacillus megatherium NT2 has relatively strong resistance to reduction on selenium element, can survive in high-concentration inorganic selenium, can reduce the inorganic selenium into nano-selenium, and has relatively weak capability of enriching arsenic, cadmium and lead; the selenium source of the nano-selenium organic fertilizer can be prepared by mixed fermentation with high-selenium rock powder which is calcined and activated at high temperature, so that the content of heavy metals does not exceed the national standard while the selenium content of the tea leaves is increased. The bacillus megaterium provided by the invention not only can reduce the toxicity of inorganic selenium in high-selenium rock and convert the inorganic selenium into nano-selenium, but also can stably, safely and standardly produce selenium-rich tea leaves, the heavy metal content of the tea leaves does not exceed the standard, and the bacillus megaterium has a wide market prospect.

The invention provides pantoea agglomerans and application thereof, and belongs to the technical field of microorganisms, the strain is preserved in China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.36122; the strain has very high selenium tolerance and transformation capacity, and can transform inorganic selenium (such as Se (IV)) with relatively high toxicity into organic selenium (such as selenomethionine and selenocystine) with low toxicity and zero-valent nano-selenium (Se0) in a culture medium containing sodium selenite. The pantoea agglomerans obtained by the invention has the characteristics of selenium-addictive microorganisms, and has potential application value in biotransformation of selenium, microbial synthesis of nano-selenium, and growth promotion and selenium enrichment of crops. The pantoea agglomerans can promote plant growth, a liquid fermentation process developed by the strain is utilized, the highest conversion rate of organic selenium can reach 92.5%, and the synthesis efficiency of nano-selenium reaches 88.3%. And development of organic selenium agricultural products is well facilitated.

The invention belongs to the technical field of microorganisms, and particularly relates to bifidobacterium longum with high yield of nano-selenium and application of the bifidobacterium longum in production of nano-selenium, the bifidobacterium longum with high yield of nano-selenium can convert inorganic selenium salt into nano-selenium, and the content of nano-selenium enriched in freeze-dried bacterial powder is up to 24000mg / kg. The selenium-rich feed additive can be directly used for selenium dietary supplements or selenium-rich animal product production, and has potential application prospects in the fields of selenium-rich food and feed additives.

The invention belongs to the technical field of plant active ingredient extraction, and relates to a segmented enzymolysis-cascade membrane separation extraction method of cyclocarya paliurus combined-state selenium polysaccharide and the combined-state selenium polysaccharide prepared by the method. The method comprises the following steps: firstly, constructing a first extraction system for a cyclocarya paliurus leaf raw material, carrying out first-stage enzymolysis, inactivation and solid-liquid separation, separating a first liquid phase through a nanofiltration membrane to form a low-molecular selenium-containing component flow, and excluding the low-molecular selenium-containing component flow in a subsequent alcohol precipitation and recovery step; and constructing a second extraction system from the first solid phase, carrying out second-stage enzymolysis, inactivation and solid-liquid separation, sequentially carrying out microfiltration, first ultrafiltration, second ultrafiltration and nanofiltration washing on the second liquid phase, and carrying out ethanol precipitation to obtain the combined-state selenium polysaccharide. The proportion of target molecular weight interval components in the obtained product is increased, the mass fraction of polysaccharide is increased, and the proportion of inorganic selenium, the mass fraction of protein and the ash content are reduced.

The application discloses a Limosilactobacillus reuteri strain capable of improving selenoenzyme activity and high in selenium selenocysteine, and belongs to the technical field of microorganisms.The Limosilactobacillus reuteri CCFM1303 screened by the application can efficiently enrich inorganic selenium and convert the inorganic selenium into organic selenium (selenium selenocysteine), which can be better absorbed and utilized by a body, and effectively improve selenoenzyme activity of the body.Animal experiments prove that the selenium-rich Limosilactobacillus reuteri can improve selenoenzyme activity in male mice, has higher biological activity than inorganic selenium, and meets physiological requirements of selenium supplement.The Limosilactobacillus reuteri CCFM1303 of the application can efficiently enrich inorganic selenium, produce selenium selenocysteine, and be used for preparing probiotic preparations for improving selenoenzyme activity of a body, and has great application prospects in the fields of food and medicine.

This invention discloses a highly active Lactobacillus acidophilus strain that produces high levels of organic selenium, belonging to the field of microbial technology. The Lactobacillus acidophilus CCFM1304 obtained by screening in this invention exhibits high activity and can efficiently enrich inorganic selenium, converting it into organic selenium for better absorption and utilization by the body. Specifically: (1) Lactobacillus acidophilus CCFM1304 can efficiently enrich inorganic selenium, converting it into organic selenium with an organic selenium content of 95.3%, and the content of selenocysteine ​​in the organic selenium is as high as 67.8%; (2) Lactobacillus acidophilus CCFM1304 has high activity, and the viable count of the freeze-dried bacterial powder can reach 6.5 × 10⁻⁶. 11 CFU / g. The highly active Lactobacillus CCFM1304 of this invention can efficiently enrich inorganic selenium, and has great application prospects in the field of inorganic selenium biotransformation.

The invention discloses a method for separating inorganic selenium from a selenoprotein primary product. The technical problem of separating inorganic selenium from the existing selenoprotein primary product is solved, the selenoprotein is purer, the organic selenium content (99.99%) is higher, the biological activity and effectiveness of selenium are improved, absorption and utilization of life bodies are facilitated, and the side effects of the selenium are reduced. Based on the characteristics that inorganic selenium is larger than organic selenium in density and higher in electric conductivity, the technical scheme of combining a precipitation method, an ultrafiltration method, a reverse osmosis method and a dialysis method is adopted, the inorganic selenium existing in selenoprotein is separated out, and a purer selenoprotein product is obtained. After selenium protein powder, selenium yeast powder, selenium mushroom powder, selenium algae powder and other organic selenium primary products are separated through the process steps, high-purity (95%-99.99%) selenium protein, inorganic selenium and purified water are obtained. Environmental protection requirements are considered preferentially, zero emission is achieved in the separation process, and the method is extremely environment-friendly. The selenium protein is pure, selenium supplementation is not limited any more, and selenium supplementation popularization becomes possible.

The invention discloses a selenium-rich lactobacillus plantarum LP3 and application thereof in crayfish breeding, and belongs to the technical field of crayfish breeding, the preservation number of the lactobacillus plantarum LP3 is CCTCC NO: M 20252688, and after a composite biological agent mainly composed of the lactobacillus plantarum LP3 and SeNPs synthesized in situ of the lactobacillus plantarum LP3 is added into crayfish feed for use, the selenium-rich lactobacillus plantarum LP3 is added into the crayfish feed for use, and the selenium-rich lactobacillus plantarum LP3 is added into the crayfish feed. Meanwhile, the growth of the crayfish can be remarkably promoted, the disease resistance is improved, the content of flavor peptides in muscles is increased, and the toxic risk of traditional inorganic selenium can be thoroughly avoided. According to the method, the core pain points of frequent diseases, low growth efficiency and poor meat quality in crayfish breeding are solved, and a new scheme is provided for healthy crayfish breeding.

The invention relates to the technical field of feed additives, and provides a feed additive which is composed of an organic trace element group, a medicinal and edible plant group, a functional nutrition group and a compound vitamin group according to specific parts by weight, wherein the organic trace element group comprises 550-650 g of organic selenium, 250-350 g of zinc methionine and 150-250 g of glycine iron; the medicinal and edible plant group comprises 35-45 kg of astragalus membranaceus powder, 25-35 kg of Chinese wolfberry fruit powder and 20-30 kg of Chinese yam powder; and the functional nutrition group comprises 45-65kg of spirulina powder of 100-200 meshes and 1.0-1.5 kg of soybean lecithin with the phosphatidylcholine content of more than or equal to 20%. Medicinal and edible plants and organic trace elements are combined, risk raw materials such as inorganic selenium are abandoned, and scientific synergistic matching is combined, so that the selenium content and the calcium content of egg products are stably increased, heavy metal and antibiotic residues are not detected, the cholesterol content is remarkably reduced, and the egg products completely meet the food safety standard; the core problems that a traditional additive is insufficient in safety and unstable in nutrition enrichment are solved.

The invention discloses a nano-selenium foliar fertilizer capable of rapidly increasing the selenium content of wheat and a preparation method of the nano-selenium foliar fertilizer, and belongs to the technical field of agricultural planting. The nano-selenium leaf fertilizer consists of the following components: 0.3-0.8 mM of nano-selenium, 0.1%-1% (m / v) of attapulgite irradiated by electron beams, 0.35-1.4% (m / v) of bacillus metabolite and the balance of water. The method has the beneficial effects that compared with the prior art and the traditional inorganic selenium fertilizer, the toxicity of nano-selenium is lower, and the damage to plants is smaller. In the use process, even under the condition of relatively high concentration, phytotoxicity to plants is not easy to generate; meanwhile, the nano-selenium is extremely small in particle size, is at a nano level and has a relatively large specific surface area, so that the nano-selenium can more easily penetrate through cuticles and cell walls on the surfaces of wheat leaves, and can be quickly absorbed and utilized by the wheat, the efficiency of supplementing the selenium element for the wheat is higher, and the selenium-rich wheat fertilizer is more environment-friendly.

The invention discloses an organic selenium biological bacterial fertilizer and a preparation method thereof, and relates to the technical field of agricultural fertilizer.The organic selenium biological bacterial fertilizer is prepared from, by weight, 1-20 parts of sodium selenite, 300-500 parts of carbohydrates, 0.1-0.3 part of protein, 0.1-0.3 part of iron, 0.2-0.6 part of phosphorus, 0.2-0.6 part of calcium, 4-8 parts of sodium, 4-7.8 parts of potassium and 0.4-0.8 part of magnesium. The fertilizer is prepared from the following components in parts by weight: 20-30 parts of humic acid, 3-5 parts of amino acid, 10-30 parts of humic acid, 0.5-2 parts of trace elements, 1-5 parts of a microbial agent and the balance of water, the pH is 6.0-7.5, and the water content is 25%-35%. According to the invention, the activity and stability of microbial flora can be remarkably improved, inorganic selenium is efficiently converted into organic selenium which is easily absorbed by crops, and meanwhile, comprehensive and balanced nutrition is provided for crop growth; the preparation technology is simple and easy to control, effective components in the product can be effectively reserved, stable product quality is guaranteed, large-scale production is achieved, the crop yield and quality can be improved after application, the soil ecological environment can be improved, and many defects of existing selenium fertilizer and biological bacterial fertilizer are overcome.

The present application belongs to the field of health food, and particularly relates to a preparation method of selenium-rich soybean enzymatic hydrolysate with blood lipid and blood sugar lowering function and application thereof. The present application reasonably designs the components and proportion of the selenium-rich nutrient solution, uses ultrasonic treatment to promote soybean absorption and conversion of inorganic selenium into organic selenium in cooperation with vitamins, simultaneously uses multi-enzyme cooperation, combines specific ultrasonic and enzymatic hydrolysis, significantly improves the selenium-rich capacity of soybean, shortens the production time of selenium-rich soybean and solves other problems, and the prepared selenium-rich soybean enzymatic hydrolysate has blood lipid and blood sugar lowering function. Compared with non-selenium-rich soybean enzymatic hydrolysate and conventional diet, the selenium-rich soybean enzymatic hydrolysate can simultaneously improve the blood lipid and blood sugar conditions of patients with hyperlipidemia and hyperglycemia. In addition, the selenium-rich soybean enzymatic hydrolysate produced by the method of the present application has high total selenium and organic selenium content, short production cycle, blood lipid and blood sugar lowering activity, is safe, green and environmentally friendly, and has wide application prospect.

The invention provides a bio-fertilizer for promoting plant growth and enriching selenium and a preparation method thereof, and belongs to the technical field of bio-fertilizers, the bio-fertilizer is based on a dried and sterilized mixed organic matter and a direct vat set pantoea inoculant, the mass fraction of N in the mixed organic matter is 4-8%, the mass fraction of P2O5 in the mixed organic matter is 1.6-3.3%, and the mass fraction of K2O in the mixed organic matter is 1.6-3.3%. The selenium-enriched biological fertilizer for promoting plant growth is characterized in that the selenium-enriched biological fertilizer for promoting plant growth is prepared by mixing and fermenting mixed organic matters, calcium superphosphate, sodium selenite and a direct-vat-set pantoea agglomerans fungicide, the pantoea agglomerans AKFX-001 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.36122. The strain disclosed by the invention has the effects of resisting high-concentration selenium, converting inorganic selenium into organic selenium and promoting plant growth at the same time, and a single strain realizes the functions which can be realized by a previous mixed strain, so that the strain has a very good market prospect.

The invention relates to the technical field of microorganisms and the technical field of dairy product processing, and discloses a screening method and application of selenium-rich lactobacillus delbrueckii subsp. Bulgaricus. The method comprises the steps that selenium-rich environmental soil is collected, pretreated and then inoculated to a sodium selenite-containing culture medium for enrichment culture; after gradient dilution, separation and purification, the intracellular selenium content is determined by using a diaminobenzidine colorimetric method, and a high-selenium-rich strain is screened out and identified. In the application aspect, the screened strains are induced to be rich in selenium in selenium-containing broth, washing is performed to remove residual inorganic selenium, then the strains are compounded with streptococcus thermophilus to prepare a leavening agent, and the leavening agent is inoculated into mixed emulsion to be fermented to prepare the selenium-rich yoghourt. The screening efficiency is high, and the obtained strain is high in selenium enrichment capacity; the prepared yoghurt realizes efficient conversion from inorganic selenium to organic selenium, and has the advantages of good fermentation performance, fine texture, good flavor and wide application prospect.

This invention provides a nano-selenium, its preparation method, and its applications, relating to the field of nano-selenium fertilizer technology. Using sodium selenate or sodium selenite as raw material A and thiol-functionalized graphene as raw material B, raw material A and raw material B are mixed and reacted for a certain period to obtain nano-selenium, which can be used in fertilizers, food anti-oxidation and preservation, industrial catalysis, or chemical analysis. This invention uses inorganic selenium and thiol-functionalized graphene as raw materials. Through a simple solution mixing, reaction, and settling process, the in-situ reduction of selenite by thiol-functionalized graphene and the stable loading of nano-selenium are achieved, completing the conversion from inorganic selenium to nano-selenium in one step without the need for reducing agents.

This invention relates to the field of microbiology, specifically to a marine yeast strain and its application in the preparation of nano-selenium. It proposes that this strain can efficiently synthesize nano-selenium in a culture medium containing sodium selenite, obtaining a bio-nano-selenium with an amorphous structure, a particle size distribution of 100–500 nm, a Zeta potential of -26.1 mV, and a surface coating of biomolecules. It achieves a sodium selenite reduction rate of up to 95.4% and a nano-selenium yield of 18.5 mg / g. Furthermore, the prepared nano-selenium exhibits excellent antioxidant activity both in vitro and at the cellular level, with significantly lower cytotoxicity than inorganic selenium, effectively alleviating hydrogen peroxide-induced oxidative stress. Therefore, it provides a safe, efficient, and stable novel nano-selenium feed additive and antioxidant source for aquaculture.

The invention discloses a method for extracting polysaccharide from selenium-rich rhodopseudomonas palustris fermentation broth, and belongs to the technical field of microbial fermentation engineering and biotechnology application. S2, decoloring: decoloring the primary concentrated solution by using a d-limonene and ethyl acetate mixed system under the action of aminated silicon dioxide nanoparticles to obtain a colorless and transparent polysaccharide solution; s3, precipitation and impurity removal: adding an organic acid salt composed of magnesium citrate and ferrous malate, stirring, adjusting the pH value to 4.5-5.5, reacting, and centrifuging to remove the precipitate; s4, desalting and purifying; and S5, drying. According to the method, the use of high-concentration ethanol is avoided in the whole process, the organic solvent residue in the product is extremely low, and multiple beneficial effects of zero alcohol precipitation, zero inorganic selenium residue, high polysaccharide yield and extremely high product purity are realized in the field of selenium-rich polysaccharide extraction for the first time; and reliable and irreplaceable technical support is provided for developing safe and efficient selenium-rich polysaccharide functional food.

The invention discloses a plant organic selenium supplement and a preparation method and application thereof, and belongs to the technical field of selenium-rich agricultural product planting. The plant organic selenium supplement is prepared by the following steps: heating seleninic acid and honey in an aqueous solution to react for 0.5-2 hours, adding an alkali solution to adjust the pH value to be neutral, and adding purified water to dilute. The plant organic selenium supplement is dissolved in water for dilution, uniformly stirred and sprayed on the rape after 2 months, so that the selenium content in the rape moss can reach more than 200mu g / kg, and the harm caused by inorganic selenium residue can be avoided. The method has practical significance for promoting the development of selenium-rich agricultural products.

The present application relates to the technical field of bioengineering, and particularly relates to a selenium-rich lactobacillus acidophilus and application thereof. The present application provides a selenium-rich lactobacillus acidophilus JGSLA09, and the strain is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC No. 34272. The strain provided by the present application has the key ability of efficiently converting inorganic selenium into organic selenium and nano selenium, and is applied to the directional fermentation of asparagus tea extraction liquid, and a selenium-rich fermentation product with high selenium content and excellent antioxidant activity is successfully prepared. The technical scheme effectively breaks through the technical bottleneck of traditional selenium-rich tea, that is, the dependence on natural selenium-rich raw materials, low selenium element dissolution rate and low bioavailability, and provides a reliable microbial fermentation process path for developing safe, efficient and functionally synergistic new selenium-rich food.

The application discloses a selenium-rich Phellinus baumii strain domestication, cultivation and selenium enrichment method, which follows the gradient domestication principle of low-selenium starting and gradual increase, sequentially completes selenium source domestication of the Phellinus baumii primary mother strain, secondary original strain and tertiary cultivation strain, and obtains the cultivation strain suitable for the high-selenium environment; then, the tertiary cultivation strain is taken as the inoculation source, standardized cultivation is carried out by adopting the bagged solid culture medium, and the temperature, humidity, light and air conditions are regulated and controlled in two stages of mycelium culture and fruiting body growth management; and through gradient selenium source supply, continuous selenium supplement during the cultivation period, accurate regulation and control of the environmental conditions and scientific treatment after harvesting, efficient absorption and conversion of selenium by the Phellinus baumii are realized, inorganic selenium is converted into organic selenium, and the organic selenium is enriched in the fruiting body. The application solves the problems of low selenium tolerance and poor selenium enrichment efficiency of the Phellinus baumii, the selenium-rich Phellinus baumii fruiting body has high and stable organic selenium content, and the strain domestication success rate and cultivation survival rate are greatly improved.

The invention discloses a selenium-rich compound microbial fertilizer regenerated by waste and a preparation method thereof. The selenium-rich compound microbial fertilizer comprises a basic raw material, a compound flora, a selenium element gradient conversion and solid-liquid two-phase fermentation system and a selenium element gradient loading process, wherein the basic raw material comprises agricultural waste and industrial by-products; the composite flora is prepared by the following steps: preparing bacillus mucilaginosus, bacillus subtilis and bacillus amyloliquefaciens into a mixed bacteria solution; the step of gradient conversion of the selenium element comprises the steps that zymophyte and biochar are added into the basic raw materials, and preliminary fermentation is conducted for 5-7 days in a fermentation tank. The invention relates to the technical field of selenium-rich bacterial fertilizers. The composite flora of bacillus mucilaginosus, bacillus subtilis and bacillus amyloliquefaciens is used; efficient degradation of the lignocellulose and in-situ synthesis of the organic selenium are realized through metabolism complementation of the three. Through compounding and fermentation processes of sodium selenite and selenium ore powder, inorganic selenium is converted into bio-available nano-selenium and seleno-amino acid.

The invention belongs to the technical field of bioactive substances, and particularly relates to a preparation method of a wheat oligopeptide selenium chelate. The preparation method comprises the following steps: firstly preparing wheat oligopeptide, then mixing the wheat oligopeptide with sodium selenite, carrying out chelation reaction under the assistance of supercritical fluid, and separating and purifying a product to obtain the wheat oligopeptide selenium chelate. The wheat oligopeptide and inorganic selenium are chelated, and compared with other protein peptide and selenium chelating, the wheat oligopeptide is higher in chelating rate and better in oxidation resistance. The chelating rate of the wheat oligopeptide selenium chelate obtained through the method is larger than or equal to 40%, and the DPPH free radical scavenging rate in an in-vitro anti-oxidation experiment is larger than or equal to 35%.

The invention discloses lactobacillus plantarum LPBai and application thereof, and belongs to the technical field of microorganisms. The lactobacillus plantarum LPBai is preserved in the China Center for Type Culture Collection on July 16, 2024, and the preservation number of the lactobacillus plantarum LPBai is CCTCC NO: M 20241579. The lactobacillus plantarum LPBai has high tolerance to sodium selenite, inorganic selenium can be converted into organic selenium and / or elemental selenium to be enriched in thalli, and the conversion rate reaches 88.45%-92.04%, so that the selenium element can be more safely supplemented, and the absorption and utilization rate is increased; meanwhile, the lactobacillus plantarum LPBai is high in oxidation resistance and nitrite degradation capacity, the nitrite degradation rate before selenium enrichment reaches 64.54%, the degradation rate after selenium enrichment reaches 79.11%, the lactobacillus plantarum LPBai can be used for degrading nitrite, and the degradation efficiency is improved.

The invention belongs to the technical field of microorganisms, and particularly relates to haematococcus pluvialis KM2-1 with a function of synergistically enriching organic selenium and astaxanthin and application of the haematococcus pluvialis KM2-1. One purpose of the invention is to provide an application of haematococcus pluvialis KM2-1 in enrichment of at least one of organic selenium and astaxanthin in a selenium ion stress environment, the haematococcus pluvialis KM2-1 is preserved in China Center for Type Culture Collection on April 3, 2025, and the preservation number of the haematococcus pluvialis KM2-1 is CCTCC NO: M 2025704. The KM2-1 algal strain has outstanding tolerance (1500 mg.L <-1 >) to inorganic selenium ions, and can keep vigorous growth and metabolism activity in a selenium stress environment with relatively high concentration, so that a biological foundation is laid for efficient selenium enrichment.

The invention provides a fermentation additive as well as a preparation method and application thereof, and relates to the technical field of livestock and poultry breeding. The preparation method of the fermentation additive comprises the following steps: inoculating saccharomyces cerevisiae into a composite pomace culture medium for first aerobic culture, wherein the composite pomace culture medium contains at least one of apple pomace, pear pomace, citrus pomace and grape pomace; adding an inorganic selenium compound into the culture system, and performing anaerobic culture to obtain yeast selenium pomace containing organic selenium; crushing the yeast selenium pomace to obtain a secondary fermentation substrate; adding astragalus membranaceus, liquorice, divaricate saposhnikovia root, eucommia ulmoides, hawthorn and other Chinese herbal medicines and aspergillus oryzae, and performing solid-state fermentation to obtain a solid fermentation substrate; and inoculating bacillus and lactic acid bacteria, adding a manganese source and a phosphate buffer system, and carrying out multi-strain synergistic fermentation to obtain the fermentation additive. The fermentation additive disclosed by the invention can cope with oxidative stress of laying hens, delay senescence, improve intestinal health and balance reproductive hormone, and completely replace antibiotics, so that the aims of long-period, high-quality and antibiotic-free healthy breeding of the laying hens are fulfilled.

The invention belongs to the technical field of selenium-enriched yeast preparations, and particularly relates to a yeast preparation for fermenting organic selenium-enriched soybean meal as well as a preparation method and application of the yeast preparation. The fermentation saccharomyces fermenti for fermenting the soybean meal rich in organic selenium is an AKULF01 strain, the preservation number of the fermentation saccharomyces fermenti is CGMCC (China General Microbiological Culture Collection Center) No.7.629, the preservation date of the fermentation saccharomyces fermenti is January 7, 2026, and the fermentation saccharomyces fermenti is used for converting inorganic selenium and increasing the white content of acid-soluble eggs. After soybean meal is fermented by the AKULF01 strain, the content of organic selenium reaches 18.63 mg / kg-19.56 mg / kg, the content of acid-soluble protein reaches 91.8 g / kg-125g / kg, the content of the organic selenium and the content of the acid-soluble protein in the fermented soybean meal are remarkably increased, and the AKULF01 strain can be used for preparing selenium-enriched soybean meal through fermentation.

The application provides a bacillus megaterium and application of the bacillus megaterium in preparation of nano selenium organic fertilizer for increasing selenium content of tea, and belongs to the technical field of microorganisms, wherein the bacillus megaterium NT2 is preserved in China Center for Type Culture Collection, and the preservation number is CCTCC NO:M 20251806; the bacillus megaterium NT2 has strong tolerance and reduction capacity to selenium elements, can survive in high-concentration inorganic selenium, and reduce the inorganic selenium into nano selenium, and has weak capacity to enrich arsenic, cadmium and lead; can be mixed with high-temperature calcination activated high-selenium rock powder to carry out mixed fermentation, to prepare a selenium source of nano selenium organic fertilizer, to increase the selenium content of tea while ensuring that the heavy metal content does not exceed the national standard. The bacillus megaterium provided by the application can reduce the toxicity of inorganic selenium in high-selenium rock and convert it into nano selenium, and can stably, safely and standardly produce selenium-rich tea, so that the heavy metal content of tea does not exceed the standard, and the application has a broad market prospect.

The invention discloses application of an organic selenium compound in preparation of anti-tumor angiogenesis drugs. The anti-tumor angiogenesis activity of selenium compounds (inorganic selenium, organic selenium and selenium nanoparticles) in different forms is compared by the system, the effect of organic selenium is most prominent, and the organic selenium can effectively induce active oxygen mediated apoptosis and cell cycle arrest of human umbilical vein endothelial cells (HUVECs). The organic selenium is used as a lead compound for drug design, and a good reference is provided for developing novel high-efficiency and low-toxicity anti-tumor angiogenesis drugs.

The invention relates to the technical field of crop management, in particular to a selenium-rich crop safety determination method and system and a storage medium. The method comprises the following steps: acquiring selenium element detection data of each detection part of a to-be-detected crop sample, and determining selenium form distribution corresponding to different existence forms in crops according to the selenium element detection data; calculating the relative proportion relation of organic selenium and inorganic selenium in the to-be-detected crop sample according to the selenium form distribution, and judging the safety risk level; collecting soil sample detection data corresponding to the to-be-detected crop sample, and determining the total selenium content and potential associated risk factors of the soil sample; and correcting the safety risk level judgment result according to the total selenium content of the soil sample and the potential associated risk factor, and determining the overall safety of the crop sample to be detected so as to output a corresponding safety determination report. The method is helpful for shortening the detection period and reducing the operation complexity, and is suitable for real-time monitoring and safety management of production and circulation links.