129 results about "Inorganic selenium" patented technology

The invention provides bacillus megaterium and application thereof in preparation of a nano-selenium organic fertilizer and improvement of the selenium content of tea leaves, and belongs to the technical field of microorganisms, the bacillus megaterium NT2 is preserved in the China Center for Type Culture Collection with the preservation number of CCTCC NO: M 20251806, and the bacillus megaterium NT2 is preserved in the China Center for Type Culture Collection with the preservation number of CCTCC NO: M 20251806. The bacillus megatherium NT2 has relatively strong resistance to reduction on selenium element, can survive in high-concentration inorganic selenium, can reduce the inorganic selenium into nano-selenium, and has relatively weak capability of enriching arsenic, cadmium and lead; the selenium source of the nano-selenium organic fertilizer can be prepared by mixed fermentation with high-selenium rock powder which is calcined and activated at high temperature, so that the content of heavy metals does not exceed the national standard while the selenium content of the tea leaves is increased. The bacillus megaterium provided by the invention not only can reduce the toxicity of inorganic selenium in high-selenium rock and convert the inorganic selenium into nano-selenium, but also can stably, safely and standardly produce selenium-rich tea leaves, the heavy metal content of the tea leaves does not exceed the standard, and the bacillus megaterium has a wide market prospect.

The invention provides pantoea agglomerans and application thereof, and belongs to the technical field of microorganisms, the strain is preserved in China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.36122; the strain has very high selenium tolerance and transformation capacity, and can transform inorganic selenium (such as Se (IV)) with relatively high toxicity into organic selenium (such as selenomethionine and selenocystine) with low toxicity and zero-valent nano-selenium (Se0) in a culture medium containing sodium selenite. The pantoea agglomerans obtained by the invention has the characteristics of selenium-addictive microorganisms, and has potential application value in biotransformation of selenium, microbial synthesis of nano-selenium, and growth promotion and selenium enrichment of crops. The pantoea agglomerans can promote plant growth, a liquid fermentation process developed by the strain is utilized, the highest conversion rate of organic selenium can reach 92.5%, and the synthesis efficiency of nano-selenium reaches 88.3%. And development of organic selenium agricultural products is well facilitated.

The invention belongs to the technical field of microorganisms, and particularly relates to bifidobacterium longum with high yield of nano-selenium and application of the bifidobacterium longum in production of nano-selenium, the bifidobacterium longum with high yield of nano-selenium can convert inorganic selenium salt into nano-selenium, and the content of nano-selenium enriched in freeze-dried bacterial powder is up to 24000mg / kg. The selenium-rich feed additive can be directly used for selenium dietary supplements or selenium-rich animal product production, and has potential application prospects in the fields of selenium-rich food and feed additives.

The invention discloses an application of a selenium-rich lactobacillus rhamnosus microbial inoculum in preparation of antitumor drugs, and relates to the field of biotechnology and tumor treatment, the selenium-rich lactobacillus rhamnosus is prepared through a selenium-rich fermentation process, inorganic selenium difficult to absorb can be converted into an organic form suitable for body absorption, and bioavailability is improved. The selenium-enriched lactobacillus rhamnosus disclosed by the invention can inhibit tumor growth in an oral administration manner, and a new carrier is provided for probiotic anti-tumor research.

The invention relates to the technical field of biological extraction, in particular to a preparation method of selenium polysaccharide in cardamine violifolia, which comprises the following steps: extracting selenium polysaccharide in cardamine violifolia by using a deep eutectic solvent as an extraction solvent and adopting an ultrasonic method, and removing protein in the selenium polysaccharide by using an isoelectric precipitation method and an enzymolysis method, thereby obtaining the selenium polysaccharide in cardamine violifolia. Removing water, enzymolysis product amino acid, inorganic selenium and other impurities by a membrane separation concentration system to obtain cardamine violifolia selenium polysaccharide; wherein the eutectic solvent comprises choline chloride and malonic acid in a molar ratio of 1: 1-1: 3, and the water content is 40-60% (mass percent); the compound enzyme is prepared from bromelain and papain in a mass ratio of 1: 1. Protein in the selenium polysaccharide is removed by adopting deep eutectic solvent ultrasonic extraction and an isoelectric precipitation method combined with an enzymolysis method, enzymolysis product amino acid, inorganic selenium and other impurities are removed by adopting an ultrafiltration membrane separation and concentration system, the structure of the polysaccharide is not damaged, and the high-purity cardamine violifolia selenium polysaccharide is obtained.

The present invention discloses an organic selenium microbial fertilizer and a preparation method thereof, wherein the composite bacteria are composed of Bacillus subtilis, Bacillus megaterium, Bacillus colloidus and Saccharomyces cerevisiae in a specific ratio, and work synergistically. A novel slow-release organic compound N-(2-hydroxypropyl)-3-selenopropionamidoacetate (HPSePA) is designed, which has a unique group and a slow-release mechanism. During preparation, agricultural waste is first treated to obtain organic materials, composite bacteria are cultured, and then an inorganic selenium source, a nutrient element additive and HPSePA are added, and the finished product is obtained by granulation and drying. The fertilizer can promote plant growth, efficiently utilize selenium, improve soil fertility, and enhance plant stress resistance, and has good application prospects.

The invention discloses bacillus velezensis and application thereof in selenium enrichment of crops and inhibition of disease bacteria, and belongs to the technical field of selenium-enriched biocontrol bacteria. The bacillus velezensis is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the bacillus velezensis is CGMCC No.34490. The bacillus velezensis is named as bacillus velezensis. The strain has efficient selenium-rich conversion capability, and can convert inorganic selenium (such as sodium selenite) with relatively high toxicity into organic selenium or nano-selenium particles with low toxicity or no toxicity and high bioavailability. In addition, the strain also has a better antifungal effect, and has an excellent inhibition effect on fungi such as fusarium solani, arbuscular oryzae, fusarium equiseti, fusarium oxysporum and the like, so that the purpose of biological prevention and control can be achieved, plant diseases can be prevented, and healthy growth of plant crops is facilitated.

The invention relates to the technical field of polyanionic sodium ion battery positive electrode materials, and discloses a method for coating a sodium ion battery polyanionic positive electrode material with an inorganic sulfur / selenium source. The method comprises the following steps: uniformly mixing a core material with an inorganic sulfur source or an inorganic selenium source, carrying out high-temperature calcination in a protective atmosphere, cooling, grinding and sieving to obtain the sulfur or selenium coated polyanionic sodium ion battery positive electrode material, and the core material is a compound of a polyanionic positive electrode material and carbon. The sulfur / selenium coating layer is formed on the surface of the polyanionic sodium ion battery positive electrode material, so that the electronic conductivity and the sodium ion diffusion rate of the positive electrode material can be improved, and the specific capacity, the rate capability and the cycling stability of the battery are improved; the material can be used as a protective layer to effectively isolate direct contact between a positive electrode material and electrolyte, so that the occurrence of interface side reaction is reduced; in addition, shedding of a positive active material can be reduced, and the cycle performance of the battery is improved.

The invention discloses bacillus licheniformis and application thereof, and relates to the technical field of microorganisms, the bacillus licheniformis is named as bacillus licheniformis ZY3, the preservation number of the bacillus licheniformis ZY3 is CCTCC NO: M 20241959, the preservation time is September 11, 2024, and the preservation address is China Center for Type Culture Collection. The bacillus licheniformis ZY3 can be used for efficiently reducing exogenous inorganic selenium into nano-selenium, is a non-tryptophan-dependent strain, has high indoleacetic acid secretion capacity and can be used for preparing fertilizers.

The invention belongs to the technical field of selenium-rich blueberry planting, and particularly relates to a selenium-rich blueberry cultivation method which comprises the following steps: S1, selecting a proper variety according to climate conditions of a planting field; s2, selecting a place with loose soil, rich organic matters and good drainage for planting; s3, the selected soil is subjected to impurity removal, and meanwhile protection facilities are built around the soil; s4, planting pre-selected high-quality nursery stocks in the planting furrows or holes between autumn without drought in winter and early spring germination; the invention innovatively proposes that in the early stage, the middle stage and the later stage of selenium-rich blueberry growth, the selenium absorption of blueberry leaves is realized by accurately spraying an inorganic selenium solution. And the cost is low. Meanwhile, the selenium content of the blueberries is rapidly increased. According to the method, the problems of enrichment nonuniformity and low absorptivity possibly caused by soil selenium application are solved. Therefore, the selenium content of the selenium-rich blueberries is greatly increased, and the overall quality and yield of the blueberries are remarkably improved.

The invention discloses a preparation method of selenium-enriched sea cucumber polypeptide, which comprises the following steps of: feeding sea cucumber with selenium-enriched basal feed containing organic selenium, cleaning selenium-enriched sea cucumber intestines, homogenizing, performing enzymolysis treatment, sequentially adding trypsin and papain to obtain enzymatic hydrolysate, centrifuging, taking supernate, and performing freeze drying to obtain the selenium-enriched sea cucumber polypeptide. The selenium-rich sea cucumber polypeptide capable of improving the immunity of the human body is obtained through operation such as membrane separation. The invention has the following beneficial effects: compared with inorganic selenium, organic selenium converted from selenium-enriched yeast can be better absorbed and does not generate toxic and side effects on sea cucumbers and human bodies; the content of selenium and protein in the sea cucumber is increased, and the activity of superoxide dismutase (SOD), catalase (CAT) and acid phosphatase (ACP) is increased; the active polypeptide is extracted from sea intestines of selenium-enriched sea cucumbers, and the prepared active polypeptide has the effect of improving immunity, can be used for preparing health-care products for resisting oxidation and improving immunity, and has excellent social and economic effects.

The invention belongs to microbial fermentation, and particularly relates to rhizobium massa and application of rhizobium massa in preparation of selenium-rich beta-1, 3 / alpha-1, 3-glucan fermentation liquor. The preparation method comprises the following steps: inoculating rhizobium pustatae of which the Latin name is Rhizobium push and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.34553 into a sterile fermentation culture medium containing inorganic selenium, and carrying out aerated fermentation, thereby obtaining the selenium-enriched rhizobium pustatae. The method solves the problems that in the prior art, a fermentation strain is low in tolerance to selenium element, the capacity of converting inorganic selenium into organic selenium is weak, and the like, and has the advantages that the product yield and the fermentation liquor viscosity are high, and the selenium content in the selenium-rich beta-1, 3 / alpha-1, 3-glucan product is high.

The invention relates to the technical field of microorganisms, in particular to lactobacillus plantarum, selenium-rich lactobacillus plantarum and application thereof. The lactobacillus plantarum with the preservation number of GDMCC No: 64006 is provided, selenium is enriched through the lactobacillus plantarum, and inorganic selenium is converted into biological nano-selenium which can be preserved in vivo for a longer time. The invention provides a technical scheme that lactobacillus plantarum is subjected to selenium-enriched culture to prepare the selenium-enriched lactobacillus plantarum, the elimination capability of the prepared selenium-enriched lactobacillus plantarum to free radicals DPPH and ABTS < + > and the endurance capability of the prepared selenium-enriched lactobacillus plantarum to simulated gastric juice can be remarkably improved, and the tolerance of the prepared selenium-enriched lactobacillus plantarum to cholate and simulated artificial intestinal juice cannot be reduced; meanwhile, the AIEC associated colitis can be relieved from the aspects of accelerating weight recovery, reducing colon tissue pathological damage, reducing AIEC colonization, promoting intestinal barrier repair, reducing the proinflammatory factor level, improving the selenium level, improving intestinal flora disorder and the like.

The invention relates to the technical field of determination of selenium in seawater, and provides a determination method for dissolved inorganic selenium in seawater. The determination method comprises the following steps: sampling seawater, filtering, adding concentrated hydrochloric acid with the mass concentration greater than or equal to 36%, carrying out a heating reaction at 98-105 DEG C for 12-18 min, cooling, and carrying out a constant volume to prepare a seawater sample to be detected: preparing a plurality of tetravalent selenium standard sample solutions according to gradient concentrations, drawing a standard curve, and testing the seawater sample to be detected. According to the method disclosed by the invention, seawater is treated by concentrated hydrochloric acid, excessive reduction of tetravalent selenium is avoided, a hydride generation-atomic fluorescence method is combined, and a heating reaction process is improved, so that the measurement of the dissolved selenium in the seawater is small in matrix interference, accurate in result, good in precision, high in sensitivity, low in detection limit, high in measurement speed and simple to operate.

The invention relates to the technical field of bio-organic fertilizers, in particular to a selenium-rich bio-organic fertilizer for navel oranges and a preparation method of the selenium-rich bio-organic fertilizer. The selenium-rich bio-organic fertilizer for navel oranges comprises the following raw materials in parts by mass: 20-40 parts of dried astragalus sinicus, 10-20 parts of livestock manure, 10-30 parts of plant ash, 1-5 parts of rice husk, 1-5 parts of sawdust, 3-6 parts of potassium fulvate, 1-3 parts of dendritic polyamidoamine, 5-8 parts of nano calcium carbonate, 1-5 parts of sodium lignin sulfonate, 1-2 parts of a complex microbial inoculant A, 1-2 parts of a complex microbial inoculant B and 2-4 parts of a selenium-rich functional microbial inoculant. And 0.25 to 1.5 parts of sodium alginate. The quality of the navel oranges is guaranteed, the toxic action of a large amount of inorganic selenium on the navel oranges in the previous planting process is solved, meanwhile, the fertilizer is rich in gap structure through mutual cooperation of the raw materials, the problem of soil hardening can be solved, the soil fertility is improved, the fertilizer efficiency is long and stable, the yield of the navel oranges is increased, and rapid growth of the navel oranges is promoted.

The invention belongs to the technical field of plant active ingredient extraction, and relates to a segmented enzymolysis-cascade membrane separation extraction method of cyclocarya paliurus combined-state selenium polysaccharide and the combined-state selenium polysaccharide prepared by the method. The method comprises the following steps: firstly, constructing a first extraction system for a cyclocarya paliurus leaf raw material, carrying out first-stage enzymolysis, inactivation and solid-liquid separation, separating a first liquid phase through a nanofiltration membrane to form a low-molecular selenium-containing component flow, and excluding the low-molecular selenium-containing component flow in a subsequent alcohol precipitation and recovery step; and constructing a second extraction system from the first solid phase, carrying out second-stage enzymolysis, inactivation and solid-liquid separation, sequentially carrying out microfiltration, first ultrafiltration, second ultrafiltration and nanofiltration washing on the second liquid phase, and carrying out ethanol precipitation to obtain the combined-state selenium polysaccharide. The proportion of target molecular weight interval components in the obtained product is increased, the mass fraction of polysaccharide is increased, and the proportion of inorganic selenium, the mass fraction of protein and the ash content are reduced.

The present invention discloses a Lactobacillus plantarum with the functions of selenium enrichment and nitrite degradation, named Lactobacillus plantarum h32, and its preservation number is: CGMCC No. 29092. Lactobacillus plantarum h32 has high nitrite degradation and selenium enrichment capabilities. Under certain conditions, it can not only degrade harmful substances and reduce food safety risks, but also convert inorganic selenium into organic selenium, improving the nutritional quality of food. The present invention also discloses the application of the Lactobacillus plantarum with the functions of selenium enrichment and nitrite degradation in pickles. By applying Lactobacillus plantarum h32 to produce selenium-enriched pickles, the obtained pickles have a lower nitrite content, and their selenium content, color, smell, taste, texture and tissue structure are all higher than those of naturally fermented pickles.

The invention belongs to the field of meat product processing, and particularly relates to a method for chelating inorganic selenium in amino acid chelated selenium-rich pork. The invention provides a method for chelating inorganic selenium in amino acid chelated selenium-enriched pork. The method comprises the following steps: S1, cutting the selenium-enriched pork into meat blocks of 3cm * 3cm * 2cm; s2, seasoning is added into the prepared selenium-enriched pork for pickling, lysine is added, the pH is adjusted to be 7, and the pickling time is 6 h; s3, taking the pickled selenium-enriched pork, adjusting the vacuum degree of the vacuum packaging machine to 0.09 MPa, and performing vacuum packaging; s4, cooking in a constant-temperature cooking pot at set temperature and time; the boiling temperature is 70 DEG C, and the boiling time is 30 minutes; and S5, cooling to obtain the selenium-enriched pork product. According to the method, inorganic selenium in the selenium-enriched pork can be chelated into organic selenium, so that the stability of the selenium element in the selenium-enriched pork can be enhanced, and the bioavailability of the selenium-enriched pork can be improved.

The application discloses a Limosilactobacillus reuteri strain capable of improving selenoenzyme activity and high in selenium selenocysteine, and belongs to the technical field of microorganisms.The Limosilactobacillus reuteri CCFM1303 screened by the application can efficiently enrich inorganic selenium and convert the inorganic selenium into organic selenium (selenium selenocysteine), which can be better absorbed and utilized by a body, and effectively improve selenoenzyme activity of the body.Animal experiments prove that the selenium-rich Limosilactobacillus reuteri can improve selenoenzyme activity in male mice, has higher biological activity than inorganic selenium, and meets physiological requirements of selenium supplement.The Limosilactobacillus reuteri CCFM1303 of the application can efficiently enrich inorganic selenium, produce selenium selenocysteine, and be used for preparing probiotic preparations for improving selenoenzyme activity of a body, and has great application prospects in the fields of food and medicine.

This invention discloses a highly active Lactobacillus acidophilus strain that produces high levels of organic selenium, belonging to the field of microbial technology. The Lactobacillus acidophilus CCFM1304 obtained by screening in this invention exhibits high activity and can efficiently enrich inorganic selenium, converting it into organic selenium for better absorption and utilization by the body. Specifically: (1) Lactobacillus acidophilus CCFM1304 can efficiently enrich inorganic selenium, converting it into organic selenium with an organic selenium content of 95.3%, and the content of selenocysteine ​​in the organic selenium is as high as 67.8%; (2) Lactobacillus acidophilus CCFM1304 has high activity, and the viable count of the freeze-dried bacterial powder can reach 6.5 × 10⁻⁶. 11 CFU / g. The highly active Lactobacillus CCFM1304 of this invention can efficiently enrich inorganic selenium, and has great application prospects in the field of inorganic selenium biotransformation.

The invention discloses a method for separating inorganic selenium from a selenoprotein primary product. The technical problem of separating inorganic selenium from the existing selenoprotein primary product is solved, the selenoprotein is purer, the organic selenium content (99.99%) is higher, the biological activity and effectiveness of selenium are improved, absorption and utilization of life bodies are facilitated, and the side effects of the selenium are reduced. Based on the characteristics that inorganic selenium is larger than organic selenium in density and higher in electric conductivity, the technical scheme of combining a precipitation method, an ultrafiltration method, a reverse osmosis method and a dialysis method is adopted, the inorganic selenium existing in selenoprotein is separated out, and a purer selenoprotein product is obtained. After selenium protein powder, selenium yeast powder, selenium mushroom powder, selenium algae powder and other organic selenium primary products are separated through the process steps, high-purity (95%-99.99%) selenium protein, inorganic selenium and purified water are obtained. Environmental protection requirements are considered preferentially, zero emission is achieved in the separation process, and the method is extremely environment-friendly. The selenium protein is pure, selenium supplementation is not limited any more, and selenium supplementation popularization becomes possible.

The present invention provides a selenium- and zinc-enriched yeast and its application, which relates to the field of microbial fermentation. The selenium- and zinc-enriched yeast provided by the present invention is obtained by culturing Saccharomyces cerevisiae mei2101.1 in a selenium- and zinc-enriched medium. Saccharomyces cerevisiae mei2101.1 can not only convert inorganic selenium and zinc into organic selenium and zinc simultaneously (the selenium enrichment amount is 1.99 mg / g, and the zinc enrichment amount is 48.80 mg / g), but also has high temperature resistance (resistant to high temperatures of 50-60 °C) and acid-base resistance (resistant to acidity with a pH of 4.5-5.6 and alkalinity with a pH of 11-12). When the selenium- and zinc-enriched yeast is added as a fermentation agent, selenium supplement, and zinc supplement to animal feeds that need to be granulated (such as piglet, fish, or layer chick feeds, etc.), it can resist the damage of high temperature during granulation to the yeast activity, greatly reduce the toxicity of inorganic selenium and zinc additives, and gradually replace zinc oxide and sodium selenite as an antibiotic-free livestock and poultry feed additive, having broad application prospects.

The invention discloses an anti-radiation organic selenium salt and a preparation method and application thereof in the field of functional food additives. The anti-radiation organic selenium salt is prepared from the following components in percentage by weight: basic salt: 80-95% of lake salt, rock salt, bamboo salt, sea salt or refined table salt; the organic selenium active ingredients comprise 0.1 to 2 percent of L-selenomethionine, and / or 1 to 3 percent of selenium-enriched yeast extract, and / or 0.5 to 2 percent of selenized carrageenan. Through fluidized bed coating and microcapsule technologies, the six-month selenium retention rate is greater than or equal to 95%, the problem that organic selenium is easy to degrade is solved, the stability is high, L-selenomethionine and vitamin E cooperate, and simulated digestion experiments show that the selenium bioavailability is greater than or equal to 80% and is superior to that of inorganic selenium (lt); 50%); the organic selenium scavenges free radicals, the vitamin E protects cell membranes, and the zinc and the astragalus extract enhance immunity, so that all-around radiation protection is realized; the amino acid mixture covers the bitter taste of the organic selenium, the delicate flavor score is greater than or equal to 7.5 / 10, and the palatability is good; the method can be directly integrated to an existing salt production line, and is suitable for industrial production.

The invention provides a condiment rich in organic selenium and a preparation method thereof, the condiment rich in organic selenium comprises the following components in percentage by mass: a) 10-100% of malt flour rich in organic selenium, the purity of the organic selenium is greater than or equal to 99.9% (detected by HPLC (High Performance Liquid Chromatography), based on seleno-amino acid); b) 0-90% of an auxiliary seasoning; the seasoning rich in organic selenium comprises pure malt flour rich in organic selenium or a composition of the pure malt flour rich in organic selenium and auxiliary seasoning, and the total selenium content in the seasoning is not lower than 20 micrograms / g. The method has the advantages that the organic selenium malt flour with the purity larger than or equal to 99.9% (HPLC detection) is adopted, and the toxicity risk of inorganic selenium is avoided; the freshness of the seasoning is enhanced through natural umami substances (such as selenoamino acid) of organic selenium, dependence on an artificial freshener is reduced, high-temperature treatment is not needed in the preparation process, and the activity of selenoprotein is reserved to the maximum extent; the quality guarantee period of the product can be prolonged through the homogenization stirring and sterile packaging technology, the bioavailability of selenium is improved by more than two times through the pH response type nano targeting carrier, and the selenium is prevented from being damaged by gastric acid.

The invention belongs to the technical field of selenium fertilizers, and particularly relates to a preparation method of an organic-inorganic compound selenium fertilizer based on soil healthy cultivation, which comprises the following steps: cleaning a porous carrier, depositing sodium selenate and sodium selenite, depositing bacillus on the surface, coating an organic selenium film layer, and finally coating a slow-release film on the surface. According to the invention, the defects of the existing selenium fertilizer are overcome, the sodium selenate and the sodium selenite are matched with the humic acid selenium fertilizer and the amino acid selenium fertilizer to form the organic-inorganic compound selenium fertilizer, and the organic selenium fertilizer and the inorganic selenium fertilizer are stably isolated according to the sequence of adsorption and coating, so that the gradient absorption selenium fertilizer is formed.

The invention discloses a preparation process of selenium-enriched gynostemma pentaphylla Fuzhuan tea. The preparation process comprises the following steps: selecting materials, spreading and airing, deactivating enzymes, rolling, adding selenium, carrying out pile fermentation on mucor, drying, ageing, steaming tea for the second time, pressing, shaping and growing fungus, and ageing for the second time. By adopting the mucor pile fermentation, on one hand, the added inorganic selenium is converted into organic selenium, and the organic selenium is tightly combined with the tea leaves, so that the loss of the selenium element is avoided; and on the other hand, the process of combining mucor pile fermentation with high-temperature instantaneous fixation reduces the degradation effect of gypenoside glycosidase on gypenoside, retains most of gypenoside in the gynostemma pentaphyllum, and improves the health-care effect of the gynostemma pentaphyllum Fuzhuan tea.

The invention relates to the technical field of microbiology, in particular to a marine yeast strain and application thereof in preparation of nano-selenium, and provides a method for efficiently synthesizing nano-selenium in a culture medium containing sodium selenite by using the strain. The biological nano-selenium has an amorphous structure, the particle size distribution of the biological nano-selenium is 100-500 nm, the Zeta potential of the biological nano-selenium reaches-26.1 mV, and the surface of the biological nano-selenium is coated with biological molecules. According to the method, sodium selenite reduction rate as high as 95.4% and nano-selenium yield as high as 18.5 mg / g can be achieved, meanwhile, the prepared nano-selenium shows excellent antioxidant activity in vitro and on the cellular level, the cytotoxicity of the nano-selenium is remarkably lower than that of inorganic selenium, oxidative stress induced by hydrogen peroxide can be effectively relieved, and the nano-selenium can be used for preparing a nano-selenium product. Therefore, a safe, efficient and stable novel nano-selenium feed additive and antioxidant source is provided for aquaculture.

The invention discloses an aquatic feed based on selenium yeast and application thereof, and belongs to the technical field of aquaculture. The invention relates to an aquatic feed additive based on selenium yeast, which systematically studies the influence of addition of different selenium sources (organic selenium and inorganic selenium) into feed on the growth performance, muscle nutrition, texture characteristics, serum biochemistry and main nutrient components of muscles of hybrid culter. The cultivation efficiency of the hybrid culter can be obviously optimized by enhancing the feed organic selenium, and the specific expression is as follows: the growth rate increase and the feed conversion efficiency improvement have obvious dose dependence; the selenium deposition amount and the crude protein enrichment degree in muscle tissue are synchronously improved, meanwhile, the synergistic effect of essential amino acid composition optimization (EAA / TAA value improvement) and n-3 series fatty acid characteristic enrichment is presented, and the two-way improvement of the nutritional quality and the flavor value is achieved from the molecular nutriology level. Mechanism research shows that the intervention strategy verifies the comprehensive regulation and control effect of the intervention strategy on the body health status by remarkably reducing the activity of serum transaminase (AST and ALT) and the total cholesterol concentration, effectively promoting the steady-state reconstruction of liver metabolism and activating an endogenous antioxidant defense system.

The invention discloses a selenium-rich lactobacillus plantarum LP3 and application thereof in crayfish breeding, and belongs to the technical field of crayfish breeding, the preservation number of the lactobacillus plantarum LP3 is CCTCC NO: M 20252688, and after a composite biological agent mainly composed of the lactobacillus plantarum LP3 and SeNPs synthesized in situ of the lactobacillus plantarum LP3 is added into crayfish feed for use, the selenium-rich lactobacillus plantarum LP3 is added into the crayfish feed for use, and the selenium-rich lactobacillus plantarum LP3 is added into the crayfish feed. Meanwhile, the growth of the crayfish can be remarkably promoted, the disease resistance is improved, the content of flavor peptides in muscles is increased, and the toxic risk of traditional inorganic selenium can be thoroughly avoided. According to the method, the core pain points of frequent diseases, low growth efficiency and poor meat quality in crayfish breeding are solved, and a new scheme is provided for healthy crayfish breeding.

The invention provides a selenium-rich agrocybe cylindracea cultivation method and a selenium-rich agrocybe cylindracea product, and belongs to the technical field of edible mushroom cultivation. According to the cultivation method of the selenium-rich agrocybe cylindracea, the organic peptide chelated selenium and the compost are mixed, then agrocybe cylindracea inoculation and cultivation are conducted, conventional inorganic selenium is replaced with organic selenium, and the cultivation method has the advantages that safety is achieved, the yield is increased, and the quality is improved. According to the invention, the organic peptide chelated selenium is added into a cultivation raw material, and the selenium-rich agrocybe cylindracea is produced through the transformation and fruiting of the agrocybe cylindracea hyphae by absorbing selenium. In one embodiment of the invention, when the content of the organic peptide chelated selenium is more than 900 mu g / kg of the cultivation material, the selenium content of the produced fresh agrocybe cylindracea is more than 0.1 mg / kg, which reaches the standard of selenium-rich products.