5 results about "Acid phosphatase" patented technology

The application discloses ZmBTB130 The application discloses application of a gene in regulating crop growth and development under low-phosphorus stress, and belongs to the technical field of genetic engineering. ZmBTB130 The application finds that, under normal phosphorus conditions, the growth difference between the knockout plant and the wild-type corn is not obvious; and under low-phosphorus stress conditions, compared with the wild-type corn, the root length and the plant height of the knockout plant are significantly increased, and the inorganic phosphorus content and the acid phosphatase activity in the body are significantly increased. The application provides a new improvement idea for low-phosphorus tolerance breeding and yield improvement of important food crop corn under the background of global phosphorus resource shortage.

This invention discloses a promoter specifically induced by low phosphorus in root cells and its application, relating to the field of genetic engineering technology. The key technical points are: the promoter has the nucleotide sequence shown in SEQ ID NO: 1. This invention also provides a vector containing the above promoter and its application in expressing the reporter gene GUS or the acid phosphatase gene OsPAP16 in rice. The beneficial effects of this invention are: the Pro-PRE provided by this invention is induced by low phosphorus and specifically expressed in root cells, driving the expression of exogenous genes, such as acid phosphatase or organic acid secretion proteins, under low phosphorus conditions, thereby improving the crop's tolerance to low phosphorus and providing important guidance for breeding low phosphorus-tolerant varieties.

This invention relates to a sulfate-reducing bacterium with phosphorus-solubilizing ability and its application in soil ecological restoration, belonging to the field of soil environmental microbiology technology. The sulfate-reducing bacterium described in this invention is *Desulfosporosinus* sp. QY25, which was deposited at the Guangdong Provincial Microbial Culture Collection Center on August 6, 2024, with accession number GDMCC No: 64963. This invention provides a sulfate-reducing bacterium, *Desulfosporosinus* sp. QY25, with strong dissimilatory sulfate-reducing ability, capable of converting insoluble phosphorus in the soil into soluble phosphorus that can be absorbed by plants. This strain can restore soil ecology, neutralize pH, and reduce SO4 levels. 2‑ It reduces the content of heavy metals, decreases their bioavailability, improves soil phosphorus utilization efficiency, and enhances tailings acid phosphatase activity, thus promoting healthy plant growth.

The application discloses a polychromatic visual kit for detecting acid phosphatase and construction and application thereof, and belongs to the technical field of biological detection and clinical diagnosis. The kit is based on a double-effect strategy of "substrate anchoring-microenvironment adaptation" of PtFe NPs, triggers a cascade reaction through ACP hydrolysis of pyrophosphate (PPi), and realizes color development through coupling of TMB oxidation and ammonium phosphomolybdate complexation, so that a composite color signal with double enhanced hue and color difference is formed. The kit can realize naked-eye accurate differentiation of ACP in a concentration range of 0-30 U / L, a linear equation can be established in a range of 0.5-20 U / L of the hue change, and quantitative detection can be carried out. The kit is simple to operate and has strong anti-interference capability, and solves the technical problems of weak signal and insufficient recognition degree of a traditional single color development method in a low concentration range.