36 results about "Acid phosphatase" patented technology

The invention discloses bifidobacterium longum subsp.infantis capable of promoting bone growth and a metagen thereof, and belongs to the technical field of biology. The bifidobacterium longum subsp. Infantis CCFM1445 provided by the invention can promote the increase of bone volume fraction, femoral bone trabecula thickness and far-end growth plate thickness of mammals in a growth period, reduce the levels of serum bone resorption markers I-type collagen amino-terminal peptide and tartrate-resistant acid phosphatase, improve the levels of serum bone alkaline phosphatase and parathyroid hormone, and improve the clinical application value of the bifidobacterium longum subsp. Infantis CCFM1445. And the relative abundance of the other bacteria in the intestinal flora and the levels of creatine and indole-3-lactic acid are increased. The thermally inactivated bifidobacterium longum subsp. Infantis CCFM1445 can promote the increase of the volume fraction of femoral bones and the number of bone trabecula of malnourished mice and improve the N-terminal propeptide level of serum I-type procollagen. Therefore, the medicine or health care product prepared from the bifidobacterium longum subsp. Infantis CCFM1445 can be used for assisting in regulating bone metabolism and bone mass, and has a wide market prospect.

A Bifidobacterium longum subsp. longum dipro-017. The Bifidobacterium longum subsp. longum dipro-017 used has the ability to metabolize tryptophan to produce indole-3-lactic acid, can inhibit the pathogenic bacteria Salmonella Typhimurium, Staphylococcus aureus, and Escherichia coli, and can effectively mitigate weight loss caused by ulcerative colitis, improve a DAI score, improve colon length, mitigate the content of lipopolysaccharide (LPS) in blood, and increase the level of anti-inflammatory cytokine IL-10, as well as mitigate the content of bone metabolism indicators tartrate-resistant acid phosphatase (TRAP) and N-terminal propeptide of type I procollagen (PINP) in blood.

The invention relates to sulfate reducing bacteria with phosphate solubilizing capacity and application of the sulfate reducing bacteria in soil ecological restoration, and belongs to the technical field of soil environment microorganisms. The sulfate reducing bacterium disclosed by the invention is Desulfosporosinus sp.QY25, and is preserved in the Guangdong Microbial Culture Collection Center on August 6, 2024, and the preservation number of the sulfate reducing bacterium is GDMCC No: 64963. The invention provides a sulfate reducing bacterium Desulfosporosinus sp.QY25 which is high in dissimilatory sulfate reducing capacity and can be used for converting insoluble phosphorus in soil into soluble phosphorus which can be absorbed by plants. The invention further provides a preparation method of the sulfate reducing bacterium Desulfosporosinus sp.QY25 and an application of the sulfate reducing bacterium Desulfosporosinus sp.QY25. The strain can restore soil ecology, neutralize pH, reduce the content of SO4 < 2->, reduce the bioavailability of heavy metals, improve the utilization efficiency of phosphorus in soil, improve the activity of acid phosphatase in tailings and enable plants to grow well.

The invention provides application of linariin-6-O-glucoside in preparation of a product for preventing and treating osteoporosis, and relates to the field of biological medicine. According to the invention, dyeing experiments of alkaline phosphatase, alizarin red and tartaric acid resistant acid phosphatase prove that the sesaminin-6-O-glucoside has affinity for FPPS receptors, has the capability of promoting bone marrow mesenchymal stem cells to form bone cells, is superior to that of bisphosphate, and can inhibit the generation of osteoclasts; microCT (micro computed tomography) detection results show that the sesaminin-6-O-glucoside has the effect of relieving the osteoporosis and can promote the formation of bone trabecula. According to the application disclosed by the invention, it is proved for the first time that the sesaminin-6-O-glucoside has the effect of resisting osteoclast formation, and meanwhile, the sesaminin-6-O-glucoside also has a better osteogenesis effect, so that a basis is provided for developing the sesaminin-6-O-glucoside as a potential medicine for treating osteoporosis.

This invention relates to a method for quantifying mRNA capping efficiency, the method comprising mixing a sample, an enzyme mixture, and an isotope standard solution in a buffer solution to produce an incubation mixture, the enzyme mixture comprising a nonspecific single-stranded nuclease and an acid phosphatase, and the isotope standard comprising isotopically labeled m7G and isotopically labeled 2'-O-methylated nucleoside; incubating the mixture; and analyzing the mixture using liquid chromatography-mass spectrometry to determine at least one of capping efficiency and 2-O-methyltransferase efficiency.

The invention belongs to the field of feed protein processing, and particularly relates to a method for improving the bioavailability of animal feed protein through chemical enzymolysis. The method comprises the following steps: mixing a compound protein raw material with water to form a suspension; sodium tripolyphosphate is added for phosphorylation pretreatment, so that protein is subjected to specific modification; after the pH is adjusted to be acidic, pepsase and acid phosphatase are added at the same time for an enzymolysis reaction; and after the reaction is finished, performing enzyme deactivation and drying to obtain the modified feed protein. According to the method, a specific enzyme cutting site is created on a protein molecule through chemical phosphorylation, then directional hydrolysis is achieved through efficient recognition of pepsin on the site, and meanwhile dynamic regulation and control of the hydrolysis process are achieved by means of the dephosphorylation effect of acid phosphatase. The obtained product has the outstanding advantages of high content of small peptides, good digestive absorbability, low bitterness, strong biological activity and the like, and the bioavailability of feed protein is remarkably improved.

The invention relates to an artificial phosphate-solubilizing module and application thereof in improving the nitrogen-fixing and phosphate-solubilizing capabilities of nitrogen-fixing microorganisms. The artificial phosphate solubilizing module sequentially comprises an inducible promoter element and a conservative acid phosphatase gene acpA from a pseudomonas fluorescens strain from upstream to downstream. The artificial phosphate solubilizing module is inserted into a preset target site of a host microorganism chromosome in a homologous recombination manner, so that a host has the characteristic of efficiently expressing acid phosphatase, and the organic phosphorus degradation capacity and nitrogen fixation capacity of the host are remarkably improved. The method has high application value in the field of preparation of high-efficiency microbial fertilizer products. The method opens up a new path for agricultural green sustainable development, and has extremely important scientific significance and wide application prospects.

A fertilizer is provided that promotes plant growth with limited phosphate run-off. The fertilizer has reduced run-off even when compared to a phosphorus normalized application of struvite. The fertilizer is based on suspended colloidal particles in aqueous solution. The colloidal particles have a size distribution in which 95 percent of the colloidal particles are less than 60 microns and have an atomic number ratio of magnesium:nitrogen:phosphorus of 1:0-3:0.5-3. The colloidal particles are heterogeneous and include domains of only struvite, or of struvite in combination with domains of pyrophosphate species, poly phosphate species, or combinations thereof. The fertilizer composition is formulated to include additional components while still retaining colloidal suspension storage stability. The colloidal particles are produced from concentrated solutions that facilitate industrial scale production. The colloidal particles promote bioavailability through root acid phosphatases activation of insoluble phosphorus species. The colloid domain size facilitates access and soil intercalation over run-off mechanisms.

The application discloses ZmBTB130 The application discloses application of a gene in regulating crop growth and development under low-phosphorus stress, and belongs to the technical field of genetic engineering. ZmBTB130 The application finds that, under normal phosphorus conditions, the growth difference between the knockout plant and the wild-type corn is not obvious; and under low-phosphorus stress conditions, compared with the wild-type corn, the root length and the plant height of the knockout plant are significantly increased, and the inorganic phosphorus content and the acid phosphatase activity in the body are significantly increased. The application provides a new improvement idea for low-phosphorus tolerance breeding and yield improvement of important food crop corn under the background of global phosphorus resource shortage.

The application provides a strain of mineralized soil organic phosphorus, Domibacillus indicus D99, and application of the strain in soil organic phosphorus mineralization, growth promotion of phosphorus solubilizing bacteria and plant growth promotion. The application first shows the function and role of Domibacillus indicus in soil organic phosphorus mineralization and plant growth promotion, and has important value for development of phosphorus solubilizing bacteria fertilizer and other microbial fertilizers. The strain of Domibacillus indicus D99 provided by the application has the ability of dissolving soil organic phosphorus, the acid phosphatase activity is 17.32, the alkaline phosphatase activity is 1.3, can promote plant growth, and improves the aboveground biomass by 50-115.

The invention discloses a method for visually detecting the activity of acid phosphatase. Based on the efficient oxidase-like characteristic and the unique photosensitive characteristic of CS-AgI NPs and the property that AA induces AgI photochemical reaction to generate AgNPs, a CS-AgI NPs-TMB composite sensing system is utilized, and a visual colorimetric method for detecting the activity of ACP is developed for the first time. Through combination of CS-AgI NPs and TMB, the method has the advantages of simple operation, good selectivity, visual reading of ACP and the like. The method shows a good linear relation to ACP in the range of 0-2 U / L, the detection limit is as low as 0.58 U / L, the method can be used for detecting the activity of ACP in an actual serum sample, and the result is reliable. According to the method, the application range of CS-AgINPs in visual analysis is widened, and a new method is developed for diagnosing ACP related diseases under physiological conditions.

The application discloses doxycycline for injection and a preparation method thereof, and belongs to the field of pharmaceutical preparations. The preparation contains doxycycline hydrochloride, polyethylene glycol-poly (caprolactone) and other raw materials, vitamin C phosphate is used to replace vitamin C, and an 'acid phosphatase-GSH' double-reducing agent hierarchical response nano-carrier is constructed. The preparation is prepared through an organic phase, an aqueous phase, homogenization, evaporation, centrifugation and other steps. The problems of poor stability of doxycycline and lack of specificity of release are solved, the storage period is extended to 36 months, the release at an infected site reaches 85% in 48 hours, the release at normal tissue is less than 20%, and precise targeted drug release is realized.

The invention discloses a rice OsHAD6 gene mutant had6 and application thereof, and relates to the technical field of biotechnology and plant genetic engineering, the technical scheme is as follows: the rice OsHAD6 gene mutant had6 is obtained by performing directed mutation on a rice acid phosphatase OsHAD6 gene through a gene editing technology, a mutant vector is constructed, and the rice OsHAD6 gene mutant had6 is obtained. A transgenic rice material is obtained by using a transgenic technology. Phosphorus treatment at different levels and phosphorus content determination are carried out on transgenic plants, it is found that the phosphorus content in rice plants can be remarkably improved after the gene is mutated, and the key effect of the gene in rice phosphorus metabolism is revealed. The invention has important application value in rice breeding and agricultural production.

The invention relates to a synchronous differentiation method of single-sample-derived osteoclasts and osteoblasts and application of the synchronous differentiation method, and belongs to the technical field of biology. According to the method, bone marrow cells are extracted from long bones of mammals, adherent cell populations and suspension cell populations are separated after culture, the suspension cell populations are pre-cultured, the adherent cells are differentiated into osteoblasts, and the suspension cells are differentiated into osteoclasts after pre-culture, so that two functional cells are synchronously obtained from the same sample, and the dosage of experimental animals is remarkably reduced. The invention further provides a tartrate-resistant acid phosphatase dye liquor formula and a dyeing method on the basis, the dyeing time is shortened to be within 10 minutes, a high-risk concentrated hydrochloric acid reagent is not needed, heating at 37 DEG C is not needed, operation is easy and convenient, cost is low, and safety is high. According to the scheme, the problems of different sample sources, tedious dyeing process, long period, high cost and the like in the traditional technology are effectively solved, and an efficient and reliable experimental tool is provided for basic researches of bone development, osteoporosis and the like.

This invention relates to the field of in vitro diagnostics, and particularly to a combined diagnostic kit for prostate diseases. The invention provides reagents including pH, peroxidase, elastase, lecithin bodies, citric acid, zinc, and acid phosphatase under mild reaction conditions, enabling rapid differentiation of inflammatory and non-inflammatory prostatitis, benign prostatic hyperplasia, and non-prostatic genital tract infections using a single test card. Through extensive experimentation, this invention has developed a one-step reaction scheme suitable for the dry chemical determination of acid phosphatase and lecithin bodies in seminal plasma. This method eliminates the need for a stop solution, achieving a one-step reaction under mild conditions that will not cause damage to operators or fully automated instruments. The samples used are seminal plasma or semen, allowing for large sample volumes and easy acquisition, significantly improving diagnostic efficiency for doctors and patient comfort.

The invention discloses a synchronous staining method for pasteurization and acid phosphatase. The synchronous staining method comprises the following steps: placing a 10ml sample bottle in a sample plate; opening a cover plate of the centrifugal machine, and symmetrically placing required centrifugal tubes; the glass slides are placed in the clamping grooves of the slide preparation plate in sequence, each glass slide needs to be fixedly provided with two slide preparation bins used for receiving samples and staining fluid, and a filter screen is placed on each slide preparation bin; the method comprises the following steps: injecting a separation liquid into a centrifuge tube, sucking a fixed volume of sample liquid from a sample bottle through a suction nozzle, injecting the sample liquid into the centrifuge tube, putting the centrifuge tube into a centrifuge according to a serial number sequence, ensuring symmetrical balance, and starting the centrifuge for centrifugation. The double-staining process is integrated on the single slide, so that the complexity of sample preparation is reduced, the sample dosage is reduced, and the diagnosis efficiency is improved.

The invention discloses a tomato acid phosphatase promoter and application thereof. The invention provides a DNA (Deoxyribonucleic Acid) molecule which is any one of the following: (A1) a DNA molecule as shown in SEQ ID No.1; (A2) a DNA molecule which has more than 99%, more than 95%, more than 90%, more than 85% or more than 80% of homology with the nucleotide sequence limited by (A1) and has a promoter function; and (A3) a DNA molecule which is hybridized with the nucleotide sequence defined by (A1) or (A2) under strict conditions and has a promoter function. The DNA molecule is a root specific expression promoter, and a key promoter resource is provided for accurately improving the tomato phosphorus absorption efficiency through genetic engineering and enhancing rhizosphere stress resistance.

The present invention relates to microbiological methods and media dedicated for the non-denaturing detection, differentiation and / or identification of Candida spp. Yeasts, in particular Candida auricula, Candida albicans, Candida glabrata, Candida tropicalis, Candida parapsilosis and Candida krusei. The invention more specifically relates to a method for identifying a yeast of the genus Candida, optionally present in a biological sample, comprising the steps of: culturing the yeast to be identified on or in a culture medium comprising:-a nutritional ingredient capable of allowing the development and growth of a yeast of the genus Candida; and-an alpha-glucosidase inducer and, optionally, an alpha-glucosidase repressor; -an acid phosphatase inducer and a phosphatase repressor; a-N-acetyl-beta-glucosaminase inducer, and a method for producing the same; and-detecting any alpha-glucosidase, acid phosphatase and / or N-acetyl-beta-glucosaminase activity expressed by said yeast; and-identifying the species to which said yeast belongs, based on the activity of alpha-glucosidase, acid phosphatase and / or N-acetyl-beta-glucosaminase actually expressed by said yeast.

The invention belongs to the technical field of gene engineering, and provides a rice transcription factor OsbHLH18 responding to phosphorus deficiency stress and application thereof, the transcription factor OsbHLH18 is subjected to overexpression to be applied to increase of the phosphorus content of rice, an expression mode of the OsbHLH18 is explored, the effect of an overexpression OsbHLH18 carrier on rice growth is evaluated, and the result shows that the OsbHLH18 is a transcription factor positioned on a cell nucleus, and the OsbHLH18 is a transcription factor positioned on a cell nucleus. The transcription activity is realized; osbHLH18 is subjected to phosphorus deficiency induced expression on leaves and roots of rice, and the phosphorus deficiency induced expression is faster and higher in response to the roots; the plant height of the overexpressed OsbHLH18 rice is higher than that of a wild type, the overexpressed OsbHLH18 can regulate and control expression of downstream acid phosphatase genes, then the activity of acid phosphatase of root systems and root surfaces is improved, the phosphorus deficiency stress adaptability of the rice is relieved by degrading organic phosphorus, and the phosphorus content of the rice is improved.

The invention discloses a rice OsHAD5 mutant and application thereof, and relates to the technical field of biotechnology and plant genetic engineering, and the technical scheme is characterized in that the rice acid phosphatase OsHAD5 gene is subjected to directed mutation through a gene editing technology, and the rice acid phosphatase OsHAD5 mutant is obtained by performing different levels of phosphorus treatment and phosphorus content determination on a mutant plant. It is found that the phosphorus content in rice leaves can be remarkably increased after the gene is mutated, and the key effect of the gene in rice phosphorus metabolism is revealed. The invention has important application value in rice breeding and agricultural production, can effectively improve the phosphorus utilization efficiency of rice and reduce the use of phosphate fertilizer, and has wide application prospect.

The invention discloses a recombinant acid phosphatase mutant, a coding gene and application of the recombinant acid phosphatase mutant, and belongs to the technical field of enzyme engineering. Existing acid phosphatase is screened, an acid phosphatase mutant with high activity and high substrate conversion rate is constructed, the acid phosphatase mutant is expressed in escherichia coli, and a cell catalyst is prepared and used for catalytic preparation of pyridoxal phosphate. The invention further provides a novel approach for synthesizing pyridoxal phosphate by microorganisms, and the conversion rate of preparing pyridoxal phosphate by utilizing pyridoxal through recombination of expression mutants can reach 57.0%.

This invention discloses a promoter specifically induced by low phosphorus in root cells and its application, relating to the field of genetic engineering technology. The key technical points are: the promoter has the nucleotide sequence shown in SEQ ID NO: 1. This invention also provides a vector containing the above promoter and its application in expressing the reporter gene GUS or the acid phosphatase gene OsPAP16 in rice. The beneficial effects of this invention are: the Pro-PRE provided by this invention is induced by low phosphorus and specifically expressed in root cells, driving the expression of exogenous genes, such as acid phosphatase or organic acid secretion proteins, under low phosphorus conditions, thereby improving the crop's tolerance to low phosphorus and providing important guidance for breeding low phosphorus-tolerant varieties.

The invention provides an application of FeCoP-NC diatomic nano-enzyme in organophosphorus pesticide residue detection, a water sample to be detected is contacted with a chromogenic reagent and FeCoP-NC diatomic nano-enzyme to generate chromogenic reaction, the content of organophosphorus pesticide is calculated according to the chromogenic intensity, or the content of the organophosphorus pesticide is calculated according to the chromogenic intensity, or the content of the organophosphorus pesticide is calculated according to the chromogenic intensity. A to-be-detected water sample is mixed with FeCoP-NC diatomic nano-enzyme, acid phosphatase, L-ascorbic acid-2-trisodium phosphate and a color developing reagent for incubation, the content of the organophosphorus pesticide is calculated according to the absorbance value of reaction liquid obtained after reaction, the FeCoP-NC diatomic nano-enzyme has FeN3P-CoN3P active sites, has oxidoid enzyme activity, and can be used for detecting the content of the organophosphorus pesticide, and the content of the organophosphorus pesticide is calculated according to the absorbance value of the reaction liquid obtained after reaction. And the chromogenic reagent contains a chromogenic substrate of peroxidase. The method can be used for rapidly detecting one or more organophosphorus pesticide residues.

The invention discloses an application of a hydrogen bond organic framework nano-enzyme in detection of acid phosphatase, and the nano-enzyme is prepared by taking H4TBAPy as a monomer, successfully synthesizing a hydrogen bond organic framework (HOFs), and developing an innovative HOFs (at) Fe nano-enzyme through a Fe < 3 + > doping technology. The nano enzyme shows excellent peroxidase-like activity under a physiological condition. Compared with a traditional method, the scheme has the advantages of simplicity and convenience in operation, low material synthesis difficulty, mild reaction conditions, no need of fluorescent labeling and the like, and shows excellent selectivity and low detection limit. It is worthy of noting that the novel sensor integrating multiple functions of loading, catalysis and sensing is successfully developed by using the frequently neglected multifunctional characteristic of the hydrogen bond organic framework nano material. The method shows huge application potential in the field of biomedical bedside diagnosis.

The application discloses a method for promoting growth of peanuts in low-phosphorus soil environment by adding exogenous acid phosphatase, which comprises the following steps: sowing peanut seeds in low-phosphorus soil, after one month of sowing, configuring an acid phosphatase aqueous solution with a concentration of 100 U / mL, and uniformly applying the acid phosphatase aqueous solution into the low-phosphorus soil at a rate of 10 U / kg of soil; detecting photosynthetic indexes of the plants two weeks after the application of the acid phosphatase aqueous solution; after three months of sowing the peanuts, detecting changes in phenotypes and soil chemical properties of the peanut plant samples, and detecting changes in phosphorus-dissolving and nitrogen-fixing bacteria in the rhizosphere soil of the peanuts. The method is scientific and effective, simple to operate, and can provide planting techniques for the growth of peanuts in low-phosphorus nutrient soil, and provide technical support for reducing fertilization and increasing efficiency in peanut planting.

This invention relates to the field of peptide preparation technology, specifically disclosing an immune-enhancing polypeptide derived from *Houttuynia cordata*, its preparation method, and its applications. The amino acid sequence of the polypeptide is shown in SEQ ID NO.1. This invention obtains the polypeptide through enzymatic extraction, chromatographic separation and purification, activity tracking screening, and mass spectrometry identification. Zebrafish experiments have confirmed that this polypeptide can significantly increase the activities of alkaline phosphatase, acid phosphatase, superoxide dismutase, and lysozyme content, exhibiting excellent immune-enhancing activity. In a *Shigella spp.* challenge experiment, the survival rate of zebrafish in the 200 mg / kg dose group reached 90.00%, far exceeding the 6.67% of the model control group. The *Houttuynia cordata*-derived immune-enhancing polypeptide of this invention has a well-defined structure and significant activity, and can be safely and efficiently used to enhance the immunity of aquatic animals and resist *Shigella spp.* infection, leaving no residue and unlikely to induce drug resistance, showing broad application prospects.

This invention provides a group of recombinant *E. coli* strains capable of synthesizing D-allulose, wherein the recombinant *E. coli* strains include the following modifications: (a) introduction and expression of dTDP-glucose-3-epimerase gene, NDP sugar hydrolase gene, allosugar-1 phosphate-isomerase gene, and acid phosphatase gene; (b) enhanced expression of glucose-1-phosphate thymidine transferase gene, glucose-1-phosphate thymidine transferase 1 gene, and glucose-1-phosphate thymidine transferase 2 gene. This disclosure produces recombinant *E. coli* strains capable of high-yield D-allulose production, increasing the yield of the target compound while reducing the yield of by-products, thus solving a bottleneck problem in industrial production and enabling large-scale production of D-allulose.

The invention discloses doxycycline for injection and a preparation method thereof, and belongs to the field of pharmaceutical preparations. The preparation contains doxycycline hydrochloride, polyethylene glycol-polycaprolactone and other raw materials, vitamin C phosphate is used for replacing vitamin C, and the'acid phosphatase-GSH 'dual-reducing-agent graded response nano-carrier is constructed. The preparation method comprises the following steps: preparing an organic phase and a water phase, homogenizing, evaporating, centrifuging and the like. The problems of poor stability and lack of release specificity of doxycycline are solved, the storage period is prolonged to 36 months, the release rate of an infected part within 48 hours reaches 85%, the release rate of a normal tissue is less than 20%, and accurate targeted drug release is realized.

This invention relates to a sulfate-reducing bacterium with phosphorus-solubilizing ability and its application in soil ecological restoration, belonging to the field of soil environmental microbiology technology. The sulfate-reducing bacterium described in this invention is *Desulfosporosinus* sp. QY25, which was deposited at the Guangdong Provincial Microbial Culture Collection Center on August 6, 2024, with accession number GDMCC No: 64963. This invention provides a sulfate-reducing bacterium, *Desulfosporosinus* sp. QY25, with strong dissimilatory sulfate-reducing ability, capable of converting insoluble phosphorus in the soil into soluble phosphorus that can be absorbed by plants. This strain can restore soil ecology, neutralize pH, and reduce SO4 levels. 2‑ It reduces the content of heavy metals, decreases their bioavailability, improves soil phosphorus utilization efficiency, and enhances tailings acid phosphatase activity, thus promoting healthy plant growth.

The invention relates to an acid phosphatase producing strain and application thereof. The classification name of the strain for producing the acid phosphatase is Acinetobacter nosocomialis LY202409191, and the preservation number of the strain is CCTCC NO: M20242039. The strain screened by the invention can efficiently produce the acid phosphatase, and the strain has a good phosphate solubilizing effect and has a relatively strong phosphate solubilizing capability on both organic phosphorus and inorganic phosphorus. The acid phosphatase produced by the strain obtained by screening has the characteristics of safety, environmental protection and high efficiency, and has wide application prospects in the fields of medical treatment, food and health.