18 results about "Acid phosphatase" patented technology

A Bifidobacterium longum subsp. longum dipro-017. The Bifidobacterium longum subsp. longum dipro-017 used has the ability to metabolize tryptophan to produce indole-3-lactic acid, can inhibit the pathogenic bacteria Salmonella Typhimurium, Staphylococcus aureus, and Escherichia coli, and can effectively mitigate weight loss caused by ulcerative colitis, improve a DAI score, improve colon length, mitigate the content of lipopolysaccharide (LPS) in blood, and increase the level of anti-inflammatory cytokine IL-10, as well as mitigate the content of bone metabolism indicators tartrate-resistant acid phosphatase (TRAP) and N-terminal propeptide of type I procollagen (PINP) in blood.

The invention relates to sulfate reducing bacteria with phosphate solubilizing capacity and application of the sulfate reducing bacteria in soil ecological restoration, and belongs to the technical field of soil environment microorganisms. The sulfate reducing bacterium disclosed by the invention is Desulfosporosinus sp.QY25, and is preserved in the Guangdong Microbial Culture Collection Center on August 6, 2024, and the preservation number of the sulfate reducing bacterium is GDMCC No: 64963. The invention provides a sulfate reducing bacterium Desulfosporosinus sp.QY25 which is high in dissimilatory sulfate reducing capacity and can be used for converting insoluble phosphorus in soil into soluble phosphorus which can be absorbed by plants. The invention further provides a preparation method of the sulfate reducing bacterium Desulfosporosinus sp.QY25 and an application of the sulfate reducing bacterium Desulfosporosinus sp.QY25. The strain can restore soil ecology, neutralize pH, reduce the content of SO4 < 2->, reduce the bioavailability of heavy metals, improve the utilization efficiency of phosphorus in soil, improve the activity of acid phosphatase in tailings and enable plants to grow well.

The invention provides application of linariin-6-O-glucoside in preparation of a product for preventing and treating osteoporosis, and relates to the field of biological medicine. According to the invention, dyeing experiments of alkaline phosphatase, alizarin red and tartaric acid resistant acid phosphatase prove that the sesaminin-6-O-glucoside has affinity for FPPS receptors, has the capability of promoting bone marrow mesenchymal stem cells to form bone cells, is superior to that of bisphosphate, and can inhibit the generation of osteoclasts; microCT (micro computed tomography) detection results show that the sesaminin-6-O-glucoside has the effect of relieving the osteoporosis and can promote the formation of bone trabecula. According to the application disclosed by the invention, it is proved for the first time that the sesaminin-6-O-glucoside has the effect of resisting osteoclast formation, and meanwhile, the sesaminin-6-O-glucoside also has a better osteogenesis effect, so that a basis is provided for developing the sesaminin-6-O-glucoside as a potential medicine for treating osteoporosis.

This invention relates to a method for quantifying mRNA capping efficiency, the method comprising mixing a sample, an enzyme mixture, and an isotope standard solution in a buffer solution to produce an incubation mixture, the enzyme mixture comprising a nonspecific single-stranded nuclease and an acid phosphatase, and the isotope standard comprising isotopically labeled m7G and isotopically labeled 2'-O-methylated nucleoside; incubating the mixture; and analyzing the mixture using liquid chromatography-mass spectrometry to determine at least one of capping efficiency and 2-O-methyltransferase efficiency.

The application discloses ZmBTB130 The application discloses application of a gene in regulating crop growth and development under low-phosphorus stress, and belongs to the technical field of genetic engineering. ZmBTB130 The application finds that, under normal phosphorus conditions, the growth difference between the knockout plant and the wild-type corn is not obvious; and under low-phosphorus stress conditions, compared with the wild-type corn, the root length and the plant height of the knockout plant are significantly increased, and the inorganic phosphorus content and the acid phosphatase activity in the body are significantly increased. The application provides a new improvement idea for low-phosphorus tolerance breeding and yield improvement of important food crop corn under the background of global phosphorus resource shortage.

This invention relates to the field of in vitro diagnostics, and particularly to a combined diagnostic kit for prostate diseases. The invention provides reagents including pH, peroxidase, elastase, lecithin bodies, citric acid, zinc, and acid phosphatase under mild reaction conditions, enabling rapid differentiation of inflammatory and non-inflammatory prostatitis, benign prostatic hyperplasia, and non-prostatic genital tract infections using a single test card. Through extensive experimentation, this invention has developed a one-step reaction scheme suitable for the dry chemical determination of acid phosphatase and lecithin bodies in seminal plasma. This method eliminates the need for a stop solution, achieving a one-step reaction under mild conditions that will not cause damage to operators or fully automated instruments. The samples used are seminal plasma or semen, allowing for large sample volumes and easy acquisition, significantly improving diagnostic efficiency for doctors and patient comfort.

The present invention relates to microbiological methods and media dedicated for the non-denaturing detection, differentiation and / or identification of Candida spp. Yeasts, in particular Candida auricula, Candida albicans, Candida glabrata, Candida tropicalis, Candida parapsilosis and Candida krusei. The invention more specifically relates to a method for identifying a yeast of the genus Candida, optionally present in a biological sample, comprising the steps of: culturing the yeast to be identified on or in a culture medium comprising:-a nutritional ingredient capable of allowing the development and growth of a yeast of the genus Candida; and-an alpha-glucosidase inducer and, optionally, an alpha-glucosidase repressor; -an acid phosphatase inducer and a phosphatase repressor; a-N-acetyl-beta-glucosaminase inducer, and a method for producing the same; and-detecting any alpha-glucosidase, acid phosphatase and / or N-acetyl-beta-glucosaminase activity expressed by said yeast; and-identifying the species to which said yeast belongs, based on the activity of alpha-glucosidase, acid phosphatase and / or N-acetyl-beta-glucosaminase actually expressed by said yeast.

The invention belongs to the technical field of gene engineering, and provides a rice transcription factor OsbHLH18 responding to phosphorus deficiency stress and application thereof, the transcription factor OsbHLH18 is subjected to overexpression to be applied to increase of the phosphorus content of rice, an expression mode of the OsbHLH18 is explored, the effect of an overexpression OsbHLH18 carrier on rice growth is evaluated, and the result shows that the OsbHLH18 is a transcription factor positioned on a cell nucleus, and the OsbHLH18 is a transcription factor positioned on a cell nucleus. The transcription activity is realized; osbHLH18 is subjected to phosphorus deficiency induced expression on leaves and roots of rice, and the phosphorus deficiency induced expression is faster and higher in response to the roots; the plant height of the overexpressed OsbHLH18 rice is higher than that of a wild type, the overexpressed OsbHLH18 can regulate and control expression of downstream acid phosphatase genes, then the activity of acid phosphatase of root systems and root surfaces is improved, the phosphorus deficiency stress adaptability of the rice is relieved by degrading organic phosphorus, and the phosphorus content of the rice is improved.

This invention discloses a promoter specifically induced by low phosphorus in root cells and its application, relating to the field of genetic engineering technology. The key technical points are: the promoter has the nucleotide sequence shown in SEQ ID NO: 1. This invention also provides a vector containing the above promoter and its application in expressing the reporter gene GUS or the acid phosphatase gene OsPAP16 in rice. The beneficial effects of this invention are: the Pro-PRE provided by this invention is induced by low phosphorus and specifically expressed in root cells, driving the expression of exogenous genes, such as acid phosphatase or organic acid secretion proteins, under low phosphorus conditions, thereby improving the crop's tolerance to low phosphorus and providing important guidance for breeding low phosphorus-tolerant varieties.

The invention provides an application of FeCoP-NC diatomic nano-enzyme in organophosphorus pesticide residue detection, a water sample to be detected is contacted with a chromogenic reagent and FeCoP-NC diatomic nano-enzyme to generate chromogenic reaction, the content of organophosphorus pesticide is calculated according to the chromogenic intensity, or the content of the organophosphorus pesticide is calculated according to the chromogenic intensity, or the content of the organophosphorus pesticide is calculated according to the chromogenic intensity. A to-be-detected water sample is mixed with FeCoP-NC diatomic nano-enzyme, acid phosphatase, L-ascorbic acid-2-trisodium phosphate and a color developing reagent for incubation, the content of the organophosphorus pesticide is calculated according to the absorbance value of reaction liquid obtained after reaction, the FeCoP-NC diatomic nano-enzyme has FeN3P-CoN3P active sites, has oxidoid enzyme activity, and can be used for detecting the content of the organophosphorus pesticide, and the content of the organophosphorus pesticide is calculated according to the absorbance value of the reaction liquid obtained after reaction. And the chromogenic reagent contains a chromogenic substrate of peroxidase. The method can be used for rapidly detecting one or more organophosphorus pesticide residues.

This invention relates to the field of peptide preparation technology, specifically disclosing an immune-enhancing polypeptide derived from *Houttuynia cordata*, its preparation method, and its applications. The amino acid sequence of the polypeptide is shown in SEQ ID NO.1. This invention obtains the polypeptide through enzymatic extraction, chromatographic separation and purification, activity tracking screening, and mass spectrometry identification. Zebrafish experiments have confirmed that this polypeptide can significantly increase the activities of alkaline phosphatase, acid phosphatase, superoxide dismutase, and lysozyme content, exhibiting excellent immune-enhancing activity. In a *Shigella spp.* challenge experiment, the survival rate of zebrafish in the 200 mg / kg dose group reached 90.00%, far exceeding the 6.67% of the model control group. The *Houttuynia cordata*-derived immune-enhancing polypeptide of this invention has a well-defined structure and significant activity, and can be safely and efficiently used to enhance the immunity of aquatic animals and resist *Shigella spp.* infection, leaving no residue and unlikely to induce drug resistance, showing broad application prospects.

This invention provides a group of recombinant *E. coli* strains capable of synthesizing D-allulose, wherein the recombinant *E. coli* strains include the following modifications: (a) introduction and expression of dTDP-glucose-3-epimerase gene, NDP sugar hydrolase gene, allosugar-1 phosphate-isomerase gene, and acid phosphatase gene; (b) enhanced expression of glucose-1-phosphate thymidine transferase gene, glucose-1-phosphate thymidine transferase 1 gene, and glucose-1-phosphate thymidine transferase 2 gene. This disclosure produces recombinant *E. coli* strains capable of high-yield D-allulose production, increasing the yield of the target compound while reducing the yield of by-products, thus solving a bottleneck problem in industrial production and enabling large-scale production of D-allulose.

This invention relates to a sulfate-reducing bacterium with phosphorus-solubilizing ability and its application in soil ecological restoration, belonging to the field of soil environmental microbiology technology. The sulfate-reducing bacterium described in this invention is *Desulfosporosinus* sp. QY25, which was deposited at the Guangdong Provincial Microbial Culture Collection Center on August 6, 2024, with accession number GDMCC No: 64963. This invention provides a sulfate-reducing bacterium, *Desulfosporosinus* sp. QY25, with strong dissimilatory sulfate-reducing ability, capable of converting insoluble phosphorus in the soil into soluble phosphorus that can be absorbed by plants. This strain can restore soil ecology, neutralize pH, and reduce SO4 levels. 2‑ It reduces the content of heavy metals, decreases their bioavailability, improves soil phosphorus utilization efficiency, and enhances tailings acid phosphatase activity, thus promoting healthy plant growth.

The invention provides a serinol-producing escherichia coli genetic engineering strain and application thereof. The strain contains an expression plasmid vector or an expression plasmid vector combination, the vector or combination comprises a first group of polynucleotides and a second group of polynucleotides, the first group of polynucleotides comprises at least one polynucleotide encoding phosphatase, and the second group of polynucleotides comprises at least one polynucleotide encoding phosphatase. The second group of polynucleotides comprises at least one polynucleotide encoding a dihydroxyacetone phosphate transaminase; the phosphatase is selected from at least one of histidine phosphatase, acid phosphatase and alkaline phosphatase, and the dihydroxyacetone phosphate transaminase is selected from at least one of CJ1437 and CJ1361. The strain realizes efficient fermentation production of serinol, and provides a new method for biosynthesis of serinol.

The invention discloses a broad-spectrum Chinese herbal medicine compound for resisting main pathogenic bacteria of prawns as well as a preparation method and application of the broad-spectrum Chinese herbal medicine compound, and belongs to the technical field of aquaculture disease control. The compound consists of sanguisorba officinalis, myrobalan, coptis chinensis and smoked plum in a mass ratio of 1: 4: 4, and is prepared by the steps of crushing and sieving the raw materials, mixing in proportion, decocting in water, concentrating and sterilizing. The compound has a remarkable broad-spectrum antibacterial effect on six main pathogenic bacteria causing common diseases such as penaeus vannamei royal death disease, red-leg disease, luminescence disease and enteritis disease, the average inhibition zone reaches 22.08 mm, the minimum inhibitory concentration is as low as 2.67 mg / mL, the safety is high, the median lethal dose is larger than 10000 mg / kg, and the actual dosage is far lower than a toxicity threshold value. Meanwhile, the compound also can obviously improve the activity of acid phosphatase and alkaline phosphatase of the prawns to two times that of a control group, and the immune protection rate reaches 46.67%. When the feed additive is added into a prawn feed according to the addition amount of 1300-1500mg / kg, common bacterial diseases of prawns can be effectively prevented and treated, and hepatopancreas and midgut tissues of the prawns are not damaged.

The application discloses a polychromatic visual kit for detecting acid phosphatase and construction and application thereof, and belongs to the technical field of biological detection and clinical diagnosis. The kit is based on a double-effect strategy of "substrate anchoring-microenvironment adaptation" of PtFe NPs, triggers a cascade reaction through ACP hydrolysis of pyrophosphate (PPi), and realizes color development through coupling of TMB oxidation and ammonium phosphomolybdate complexation, so that a composite color signal with double enhanced hue and color difference is formed. The kit can realize naked-eye accurate differentiation of ACP in a concentration range of 0-30 U / L, a linear equation can be established in a range of 0.5-20 U / L of the hue change, and quantitative detection can be carried out. The kit is simple to operate and has strong anti-interference capability, and solves the technical problems of weak signal and insufficient recognition degree of a traditional single color development method in a low concentration range.

The application discloses a Bifidobacterium breve and application thereof, and belongs to the technical field of microorganisms. The Bifidobacterium breve CCFM1310 provided by the application has the functions of improving immune organs, increasing the number of white blood cells in blood, up-regulating the activities of acid phosphatase (ACP) and lactate dehydrogenase (LDH), increasing the contents of immunoglobulin IgA, IgG and IgM in serum, regulating intestinal flora and the like, and can improve the immune capacity of the body by increasing the organ index, non-specific immune response and specific immune response of the body. The strain provided by the application can be used as a daily intake of probiotics to replace drugs for improving the immune capacity of the human body, and can be used as a new choice for treating patients with low immunity.

The invention discloses application of an acid phosphatase OsPAP23 gene in efficient rice phosphorus breeding, the acid phosphatase OsPAP23 is found to have organophosphorus hydrolase activity and participate in rice phosphorus absorption and utilization regulation for the first time, expression of the acid phosphatase OsPAP23 gene is increased in a plant body, the acid phosphatase activity of rice is improved, and the yield of rice phosphorus is increased. Further, the utilization efficiency of phosphorus in the body and the utilization efficiency of organic phosphorus are improved. According to the invention, research on phosphorus absorption and transport regulation and control mechanisms is enriched, a reference mechanism is provided for corresponding phosphorus deficiency stress of rice, and the created PAP23 overexpression strain is expected to show growth and yield advantages in fields with organic fertilizers.