70 results about "Acid phosphatase" patented technology

The invention provides a plant lactobacillus TH13, and the preservation number of the plant lactobacillus TH13 is CGMCC (China General Microbiological Culture Collection Center) NO.32112. The plant lactobacillus TH13 provided by the invention can be used for fermenting folium cortex eucommiae and enzymatically hydrolyzed bone meal, and the fermentation product of the plant lactobacillus TH13 has high content of chlorogenic acid, proline, glycine and lysine. The fermentation product can significantly improve the content of alkaline phosphatase (ALP) and osteocalcin (OCN) in mouse serum and reduce the content of tartrate-resistant acid phosphatase (TRACP). Therefore, the fermentation product can significantly improve the bone mineral density of the femur, increase the number of bone trabecula and significantly reduce the separation degree of the bone trabecula, thereby promoting bone formation of a mouse with senile osteoporosis, inhibiting bone resorption and improving senile bone loss and bone microstructure deterioration. The preparation method of the probiotic preparation containing the plant lactobacillus and the probiotic fermentation product is simple, and large-scale production can be realized.

The invention discloses a ganoderma lucidum fermentation liquid capable of improving the content of total triterpenes and immunity and a preparation method and application thereof, the preparation method comprises the following steps: heating a ganoderma lucidum liquid, and carrying out hot water extraction to obtain a ganoderma lucidum extracting liquid; heating the lucid ganoderma extracting solution to be boiled, after sterilization is finished, after the lucid ganoderma liquid is cooled, adding lactic acid bacteria powder in a specific proportion for fermentation to obtain lucid ganoderma fermentation liquid; and carrying out solid-liquid separation on the fermentation liquor to obtain fermentation supernate, and sterilizing the ganoderma lucidum fermentation liquor to obtain the sterilized ganoderma lucidum fermentation liquor. According to the preparation method disclosed by the invention, the content of total triterpenes in the ganoderma lucidum fermentation liquor is increased, the immunomodulatory activity of the ganoderma lucidum product can be enhanced, and the ganoderma lucidum product has relatively high effects of resisting oxidation, improving the activity of phagocytes, improving the activity of acid phosphatase, improving the immunity and the like, and has a wide application prospect and market potential.

The invention relates to the technical field of new strains, and provides a new strain which is screened from Xinjiang saline-alkali soil, is classified and named as sphingomonas sp. And is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCCNO.32220. Research finds that the strain can remarkably reduce the soil conductivity, the exchangeable sodium content, the alkalization degree and the total salt content, so that the soil quality is improved. The organic matter content and the acid phosphatase activity of the soil are remarkably improved, and the quality of the saline-alkali soil can be efficiently improved. Meanwhile, the invention provides a compound bacterial fertilizer for effectively improving saline-alkali soil, the compound bacterial fertilizer comprises bacillus subtilis, Exiguobacterium acetylbacter, Bacillus velezensis, Pseudomonas fluorescens, Rhodococcus capsulatus and a new strain Sphingomonas sp., and research finds that compared with a single bacterial solution, the compound bacterial solution has a more prominent remediation effect on saline-alkali soil.

The invention discloses a comprehensive prevention and control method for tobacco root knot nematode disease containing a biological agent. The method comprises the steps of crop rotation of tobacco and buckwheat / quinoa, host resistance induction of the microbial agent in the seedling raising period, tobacco planting soil quality improvement in the transplanting period and the like. The agronomic characters of tobacco seedlings are effectively improved, and the physical characters of soil are improved; the chemical properties of soil pH, alkali-hydrolyzable nitrogen, available phosphorus, quick-acting potassium, exchangeable calcium, exchangeable magnesium and the like are improved; the activity of soil urease, sucrase, acid phosphatase, catalase and peroxidase is improved; the content of water-soluble phenols, compound phenols and total phenols in soil is reduced; the disease index of the tobacco root knot nematode disease can be remarkably reduced, the survival rate of tobacco is increased, the application amount of chemical agents is reduced, and the healthy and sustainable development of the tobacco industry is promoted.

The invention discloses bifidobacterium longum subsp.infantis capable of promoting bone growth and a metagen thereof, and belongs to the technical field of biology. The bifidobacterium longum subsp. Infantis CCFM1445 provided by the invention can promote the increase of bone volume fraction, femoral bone trabecula thickness and far-end growth plate thickness of mammals in a growth period, reduce the levels of serum bone resorption markers I-type collagen amino-terminal peptide and tartrate-resistant acid phosphatase, improve the levels of serum bone alkaline phosphatase and parathyroid hormone, and improve the clinical application value of the bifidobacterium longum subsp. Infantis CCFM1445. And the relative abundance of the other bacteria in the intestinal flora and the levels of creatine and indole-3-lactic acid are increased. The thermally inactivated bifidobacterium longum subsp. Infantis CCFM1445 can promote the increase of the volume fraction of femoral bones and the number of bone trabecula of malnourished mice and improve the N-terminal propeptide level of serum I-type procollagen. Therefore, the medicine or health care product prepared from the bifidobacterium longum subsp. Infantis CCFM1445 can be used for assisting in regulating bone metabolism and bone mass, and has a wide market prospect.

A Bifidobacterium longum subsp. longum dipro-017. The Bifidobacterium longum subsp. longum dipro-017 used has the ability to metabolize tryptophan to produce indole-3-lactic acid, can inhibit the pathogenic bacteria Salmonella Typhimurium, Staphylococcus aureus, and Escherichia coli, and can effectively mitigate weight loss caused by ulcerative colitis, improve a DAI score, improve colon length, mitigate the content of lipopolysaccharide (LPS) in blood, and increase the level of anti-inflammatory cytokine IL-10, as well as mitigate the content of bone metabolism indicators tartrate-resistant acid phosphatase (TRAP) and N-terminal propeptide of type I procollagen (PINP) in blood.

The disclosure relates to rare earth element (REE) phosphates and methods for making the same. The REE phosphates can be formed by combining a REE sulfate with a phosphate source and a biological catalyst, for example an acid phosphatase.

The invention provides a medicinal and edible composition for preventing and treating osteoporosis and a preparation method thereof. The medicinal and edible composition comprises the following raw materials in parts by weight: 15-24 parts of prepared rehmannia root, 12-15 parts of dogwood, 12-20 parts of Chinese yam, 15-20 parts of astragalus membranaceus, 3-10 parts of angelica sinensis and 10-20 parts of folium cortex eucommiae; the medicinal and edible composition provided by the invention promotes osteoblast differentiation, inhibits osteoclast absorption, regulates bone metabolism balance, increases the maximum load of thighbone, and improves bone density and bone strength by increasing osteoprotegerin and reducing type I collagen C-terminal peptide and tartrate-resistant acid phosphatase. The medicinal and edible composition disclosed by the invention is high in safety, can be developed into medicines, health care products or functional foods, and is applied to prevention and treatment of osteoporosis.

The invention provides a group of recombinant Escherichia coli for forming D-psicose. The recombinant Escherichia coli comprises the following multiple modifications: (a) introducing and expressing a dTDP-glucose-3-epimerase gene, an NDP glycohydrolase gene, an allose-1 phosphate-isomerase gene and an acid phosphatase gene; and (b) enhancing the expression of the glucose phosphate mutase gene, the expression of the glucose 1-phosphate thymine transferase 1 gene and the expression of the glucose 1-phosphate thymine transferase 2 gene. The recombinant Escherichia coli capable of producing D-psicose at high yield is prepared, the yield of by-products is reduced while the yield of a target object is increased, the bottleneck problem in industrial production is solved, and the recombinant Escherichia coli can be used for mass production of D-psicose.

The invention discloses a tetrahydro-beta-carboline compound as well as a preparation method and application thereof. The tetrahydro-beta-carboline compound is shown as a formula P. The tetrahydro-beta-carboline compound has good RANKL inhibitory activity, the preparation method is simple, raw materials are easy to obtain, and the yield is high. The inhibition activity of the compound on generation of RANKL-induced osteoclasts is determined through a tartrate acid phosphatase (TRAP) staining experiment, it is determined through a surface plasmon resonance (SPR) that the compound has high combining capacity with RANKL protein, and the compound can be used for preparing drugs for preventing and / or treating diseases caused by abnormal osteoclast activity. # imgabs0 #

The invention relates to sulfate reducing bacteria with phosphate solubilizing capacity and application of the sulfate reducing bacteria in soil ecological restoration, and belongs to the technical field of soil environment microorganisms. The sulfate reducing bacterium disclosed by the invention is Desulfosporosinus sp.QY25, and is preserved in the Guangdong Microbial Culture Collection Center on August 6, 2024, and the preservation number of the sulfate reducing bacterium is GDMCC No: 64963. The invention provides a sulfate reducing bacterium Desulfosporosinus sp.QY25 which is high in dissimilatory sulfate reducing capacity and can be used for converting insoluble phosphorus in soil into soluble phosphorus which can be absorbed by plants. The invention further provides a preparation method of the sulfate reducing bacterium Desulfosporosinus sp.QY25 and an application of the sulfate reducing bacterium Desulfosporosinus sp.QY25. The strain can restore soil ecology, neutralize pH, reduce the content of SO4 < 2->, reduce the bioavailability of heavy metals, improve the utilization efficiency of phosphorus in soil, improve the activity of acid phosphatase in tailings and enable plants to grow well.

The invention discloses a preparation method of selenium-enriched sea cucumber polypeptide, which comprises the following steps of: feeding sea cucumber with selenium-enriched basal feed containing organic selenium, cleaning selenium-enriched sea cucumber intestines, homogenizing, performing enzymolysis treatment, sequentially adding trypsin and papain to obtain enzymatic hydrolysate, centrifuging, taking supernate, and performing freeze drying to obtain the selenium-enriched sea cucumber polypeptide. The selenium-rich sea cucumber polypeptide capable of improving the immunity of the human body is obtained through operation such as membrane separation. The invention has the following beneficial effects: compared with inorganic selenium, organic selenium converted from selenium-enriched yeast can be better absorbed and does not generate toxic and side effects on sea cucumbers and human bodies; the content of selenium and protein in the sea cucumber is increased, and the activity of superoxide dismutase (SOD), catalase (CAT) and acid phosphatase (ACP) is increased; the active polypeptide is extracted from sea intestines of selenium-enriched sea cucumbers, and the prepared active polypeptide has the effect of improving immunity, can be used for preparing health-care products for resisting oxidation and improving immunity, and has excellent social and economic effects.

The invention provides application of linariin-6-O-glucoside in preparation of a product for preventing and treating osteoporosis, and relates to the field of biological medicine. According to the invention, dyeing experiments of alkaline phosphatase, alizarin red and tartaric acid resistant acid phosphatase prove that the sesaminin-6-O-glucoside has affinity for FPPS receptors, has the capability of promoting bone marrow mesenchymal stem cells to form bone cells, is superior to that of bisphosphate, and can inhibit the generation of osteoclasts; microCT (micro computed tomography) detection results show that the sesaminin-6-O-glucoside has the effect of relieving the osteoporosis and can promote the formation of bone trabecula. According to the application disclosed by the invention, it is proved for the first time that the sesaminin-6-O-glucoside has the effect of resisting osteoclast formation, and meanwhile, the sesaminin-6-O-glucoside also has a better osteogenesis effect, so that a basis is provided for developing the sesaminin-6-O-glucoside as a potential medicine for treating osteoporosis.

The present invention belongs to the field of medicine and relates to the use of benzothiazinone vinylidene indene compounds in the preparation of osteoclast differentiation inhibitors. The present invention uses tartrate-resistant acid phosphatase (TRAP) staining and reverse transcription real-time fluorescence quantitative polymerase chain reaction (RT-qPCR) technology to confirm for the first time that this series of compounds can significantly inhibit RANKL-induced osteoclast differentiation and the formation of multinucleated osteoclasts, and are non-cytotoxic at concentrations of 5μM and below. Based on these findings, the benzothiazinone vinylidene indene compounds provided by the present invention can be used as candidate drug molecules for novel osteoclast differentiation inhibitors, for the prevention and / or treatment of bone metabolic diseases caused by excessive activation of osteoclasts, such as osteoporosis, periprosthetic bone dissolution, tumor metastasis bone destruction, and rheumatoid arthritis.

This invention relates to a method for quantifying mRNA capping efficiency, the method comprising mixing a sample, an enzyme mixture, and an isotope standard solution in a buffer solution to produce an incubation mixture, the enzyme mixture comprising a nonspecific single-stranded nuclease and an acid phosphatase, and the isotope standard comprising isotopically labeled m7G and isotopically labeled 2'-O-methylated nucleoside; incubating the mixture; and analyzing the mixture using liquid chromatography-mass spectrometry to determine at least one of capping efficiency and 2-O-methyltransferase efficiency.

The invention belongs to the field of feed protein processing, and particularly relates to a method for improving the bioavailability of animal feed protein through chemical enzymolysis. The method comprises the following steps: mixing a compound protein raw material with water to form a suspension; sodium tripolyphosphate is added for phosphorylation pretreatment, so that protein is subjected to specific modification; after the pH is adjusted to be acidic, pepsase and acid phosphatase are added at the same time for an enzymolysis reaction; and after the reaction is finished, performing enzyme deactivation and drying to obtain the modified feed protein. According to the method, a specific enzyme cutting site is created on a protein molecule through chemical phosphorylation, then directional hydrolysis is achieved through efficient recognition of pepsin on the site, and meanwhile dynamic regulation and control of the hydrolysis process are achieved by means of the dephosphorylation effect of acid phosphatase. The obtained product has the outstanding advantages of high content of small peptides, good digestive absorbability, low bitterness, strong biological activity and the like, and the bioavailability of feed protein is remarkably improved.

The invention relates to an artificial phosphate-solubilizing module and application thereof in improving the nitrogen-fixing and phosphate-solubilizing capabilities of nitrogen-fixing microorganisms. The artificial phosphate solubilizing module sequentially comprises an inducible promoter element and a conservative acid phosphatase gene acpA from a pseudomonas fluorescens strain from upstream to downstream. The artificial phosphate solubilizing module is inserted into a preset target site of a host microorganism chromosome in a homologous recombination manner, so that a host has the characteristic of efficiently expressing acid phosphatase, and the organic phosphorus degradation capacity and nitrogen fixation capacity of the host are remarkably improved. The method has high application value in the field of preparation of high-efficiency microbial fertilizer products. The method opens up a new path for agricultural green sustainable development, and has extremely important scientific significance and wide application prospects.

The invention provides an application of farrerol in preparation of a medicine for preventing osteoporotic diseases caused by inflammatory bowel diseases, and belongs to the technical field of inflammatory bone disease treatment.The application comprises the steps that firstly, an ulcerative colitis (UC) mouse is constructed to simulate occurrence of the osteoporosis induced by enteritis, and the occurrence of the osteoporosis induced by enteritis is simulated through microcomputer tomography (CT), hematoxylin and eosin (Hamp; e) and tartrate-resistant acid phosphatase (TRAP) staining are used for analyzing the bone destruction degree and the number of osteoclasts, and the influence of the farrerol on osteoclast generation and bone loss is detected. The influence of the farrerol on the inflammatory bowel disease is analyzed through 16S rRNA (ribosomal Ribonucleic Acid) sequencing. In addition, PI3K-AKT and MAPK signal pathways related to osteoclast differentiation of the farrerol are detected through WB and PCR experiments to clarify the basic molecular mechanism of the farrerol for preventing osteoporosis. Results show that the farrerol can improve bone loss caused by enteritis, recover intestinal flora composition and repair intestinal barriers.

The invention discloses araban obtained from fructus lycii and application of the araban. According to the molecular structure of the araban, a main chain is formed by repeated-> 5)-alpha-Araf-(1-> structural units, and the C-2 position of each sugar residue is substituted by T-alpha-Araf-(1-> structural units. According to the method, free protein is removed through a water extraction and alcohol precipitation method and an alkali solution method, graded alcohol precipitation and purification are combined, and the araban component only containing arabinose is extracted and separated from the fructus lycii for the first time. Compared with an arabinogalactan component, the arabinogalactan component has the advantages that the phagocytic ability of macrophages is remarkably improved, the macrophages are induced to secrete NO, the activity of acid phosphatase is improved, the phagocytic ability of the macrophages and the activity of the acid phosphatase can be activated in an LPS-induced inflammation model, NO secretion is inhibited, and the arabinogalactan component can be used for preparing an LPS-induced inflammation model. Therefore, an anti-inflammatory effect is generated, and a better bidirectional immunoregulation effect is achieved.

A fertilizer is provided that promotes plant growth with limited phosphate run-off. The fertilizer has reduced run-off even when compared to a phosphorus normalized application of struvite. The fertilizer is based on suspended colloidal particles in aqueous solution. The colloidal particles have a size distribution in which 95 percent of the colloidal particles are less than 60 microns and have an atomic number ratio of magnesium:nitrogen:phosphorus of 1:0-3:0.5-3. The colloidal particles are heterogeneous and include domains of only struvite, or of struvite in combination with domains of pyrophosphate species, poly phosphate species, or combinations thereof. The fertilizer composition is formulated to include additional components while still retaining colloidal suspension storage stability. The colloidal particles are produced from concentrated solutions that facilitate industrial scale production. The colloidal particles promote bioavailability through root acid phosphatases activation of insoluble phosphorus species. The colloid domain size facilitates access and soil intercalation over run-off mechanisms.

The application discloses ZmBTB130 The application discloses application of a gene in regulating crop growth and development under low-phosphorus stress, and belongs to the technical field of genetic engineering. ZmBTB130 The application finds that, under normal phosphorus conditions, the growth difference between the knockout plant and the wild-type corn is not obvious; and under low-phosphorus stress conditions, compared with the wild-type corn, the root length and the plant height of the knockout plant are significantly increased, and the inorganic phosphorus content and the acid phosphatase activity in the body are significantly increased. The application provides a new improvement idea for low-phosphorus tolerance breeding and yield improvement of important food crop corn under the background of global phosphorus resource shortage.

The invention provides citrobacter mulesii and an application of the citrobacter mulesii in dissolving and dissolving phosphorus, the citrobacter mulesii is citrobacter mulesii LY2024080101 and is preserved in the China Center for Type Culture Collection, the preservation date is December 30, 2024, and the preservation number is CCTCC (China Center for Type Culture Collection) NO: M20242931. Compared with the prior art, the strain capable of producing the acid phosphatase is obtained through screening, belongs to citrobacter mulesii, can be used for producing the acid phosphatase, can be used for dissolving phosphorus and is excellent in effect. The acid phosphatase produced by the strain obtained by screening has the characteristics of safety, environmental protection and high efficiency, and has wide application prospects in the fields of medical treatment, food and health.

A polyphenol additive for improving the frozen storage quality of unwashed surimi and its application. The polyphenol additive for improving the frozen storage quality of unwashed surimi has active ingredients including polyphenols that can inhibit the activity of acid phosphatase, polyphenols that can inhibit the activity of adenosine deaminase, and polyphenols that can inhibit the activity of xanthine oxidase. The present invention also includes the application of the polyphenol additive for improving the frozen storage quality of unwashed surimi. The present invention can effectively reduce the contents of inosine and hypoxanthine in frozen unwashed surimi, delay the increase of K value, and improve the gel strength of frozen unwashed surimi. The usage method is simple and the application range is wide. It is derived from edible plants and has high safety. The product has low cost, simple processing technology, and is suitable for industrial production.

The application provides a strain of mineralized soil organic phosphorus, Domibacillus indicus D99, and application of the strain in soil organic phosphorus mineralization, growth promotion of phosphorus solubilizing bacteria and plant growth promotion. The application first shows the function and role of Domibacillus indicus in soil organic phosphorus mineralization and plant growth promotion, and has important value for development of phosphorus solubilizing bacteria fertilizer and other microbial fertilizers. The strain of Domibacillus indicus D99 provided by the application has the ability of dissolving soil organic phosphorus, the acid phosphatase activity is 17.32, the alkaline phosphatase activity is 1.3, can promote plant growth, and improves the aboveground biomass by 50-115.

The invention discloses a method for visually detecting the activity of acid phosphatase. Based on the efficient oxidase-like characteristic and the unique photosensitive characteristic of CS-AgI NPs and the property that AA induces AgI photochemical reaction to generate AgNPs, a CS-AgI NPs-TMB composite sensing system is utilized, and a visual colorimetric method for detecting the activity of ACP is developed for the first time. Through combination of CS-AgI NPs and TMB, the method has the advantages of simple operation, good selectivity, visual reading of ACP and the like. The method shows a good linear relation to ACP in the range of 0-2 U / L, the detection limit is as low as 0.58 U / L, the method can be used for detecting the activity of ACP in an actual serum sample, and the result is reliable. According to the method, the application range of CS-AgINPs in visual analysis is widened, and a new method is developed for diagnosing ACP related diseases under physiological conditions.

The present invention discloses an application of a trisubstituted pyrazole compound in the preparation of a drug for inhibiting osteoclast differentiation, and belongs to the field of medicine. The present invention uses a variety of experimental methods such as tartrate-resistant acid phosphatase (TRAP) staining, reverse transcription real-time fluorescence quantitative PCR (qPCR), protein immunoblotting (Western blot), etc., and proves for the first time that the trisubstituted pyrazole compound of the following formula can significantly increase the expression of antioxidant proteins HO-1 and Catalase, thereby effectively inhibiting the differentiation and generation of osteoclasts. Therefore, the trisubstituted pyrazole compound provided by the present invention can be used as a candidate compound for the development of a new osteoclast differentiation inhibitor to treat diseases caused by excessive osteoclast activation.

The application discloses doxycycline for injection and a preparation method thereof, and belongs to the field of pharmaceutical preparations. The preparation contains doxycycline hydrochloride, polyethylene glycol-poly (caprolactone) and other raw materials, vitamin C phosphate is used to replace vitamin C, and an 'acid phosphatase-GSH' double-reducing agent hierarchical response nano-carrier is constructed. The preparation is prepared through an organic phase, an aqueous phase, homogenization, evaporation, centrifugation and other steps. The problems of poor stability of doxycycline and lack of specificity of release are solved, the storage period is extended to 36 months, the release at an infected site reaches 85% in 48 hours, the release at normal tissue is less than 20%, and precise targeted drug release is realized.

The invention relates to microbiological methods and to culture media dedicated to the non-denaturing detection, discrimination and / or identification of yeasts of the Candida genus, in particular C. auris, C. albicans, C. glabrata, C. tropicalis, C. parapsilosis and C. krusei. The invention more particularly relates to a method for identifying yeasts of the Candida genus which are optionally present in a biological sample, comprising the following steps: - culturing the yeasts to be identified on or in a culture medium comprising: - a nutrient component that is capable of allowing the development and growth of yeasts of the Candida genus; and - an α-glucosidase inducer and optionally an α-glucosidase repressor; - an acid phosphatase inducer and a phosphatase repressor; - an N-acetyl-β-glucosaminidase inducer; and - detecting any α-glucosidase, acid phosphatase and / or N-acetyl-β-glucosaminidase activities expressed by the yeasts; and - identifying the species to which the yeasts belong on the basis of the α-glucosidase, acid phosphatase and / or N-acetyl-β-glucosaminidase activities actually expressed by the yeasts.

The invention belongs to the field of medicines, and relates to application of a benzothiazinone vinylidene indene compound in preparation of an osteoclast differentiation inhibitor. Through tartrate-resistant acid phosphatase (TRAP) staining and reverse transcription real-time fluorescent quantitative polymerase chain reaction (RT-qPCR) technologies, it is proved for the first time that the series of compounds can significantly inhibit RANKL-induced osteoclast differentiation and polykaryotic osteoclast formation, and have no cytotoxicity at the concentration of 5 [mu] M or below. Based on the discoveries, the benzothiazinone vinylidene indene compound provided by the invention can be used as a candidate drug molecule of a novel osteoclast differentiation inhibitor, and is used for preventing and / or treating osteoporosis, periprosthetic osteolysis, tumor metastasis bone destruction, rheumatoid arthritis and other bone metabolism diseases caused by overactivity of osteoclasts.

The present invention discloses a strain of Bifidobacterium longum subsp. longum dipro-017. The Bifidobacterium longum subsp. longum ([ Bifidobacterium longum subsp. longum ) dipro-017 used in the present invention has the ability to metabolize tryptophan to produce indole-3-lactic acid, can inhibit pathogenic bacteria such as Salmonella typhimurium, Staphylococcus aureus, and Escherichia coli, can effectively improve the weight loss caused by ulcerative colitis, improve the DAI score, improve the colon length, improve the content of lipopolysaccharide LPS in the blood and increase the level of anti-inflammatory cytokine IL-10, and at the same time improve the content of bone metabolism indexes tartrate-resistant acid phosphatase (TRAP) and N-terminal propeptide of type I collagen (PINP) in the blood.