5 results about "Tert-leucine" patented technology

The invention relates to a compound MDMB-BUTINACA-D3 as well as preparation and application thereof, and the structural formula of the compound MDMB-BUTINACA-D3 is as follows: 2-(1H-indazole-3-formamido)-3, 3, 4-triazole-3-carboxylic acid is obtained through condensation reaction of indazole-3-carboxylic acid and tert-leucine methyl ester hydrochloride; the preparation method comprises the following steps: adding 2-(1H-indazole-3-formamido)-3, 3-dimethyl butyric acid methyl ester into 2-(1H-indazole-3-formamido)-3, 3-dimethyl butyric acid methyl ester, adding 3-phenoxybromopropane into a Grignard reagent, carrying out a substitution reaction to obtain butoxybenzene-D4, further adding a brominating agent boron tribromide, carrying out a bromination reaction to obtain bromobutane-D3, and finally carrying out a substitution reaction on 2-(1H-indazole-3-formamido)-3, 3-dimethyl butyric acid methyl ester and bromobutane-D3 again to obtain MDMB-BUTINACA-D3. According to the method, the matrix effect can be well avoided, meanwhile, mass spectrum cross signal overlapping caused by natural isotopes can be avoided, and the mass spectrum quantitative analysis precision can be remarkably improved when the method is used as an internal standard substance for MS quantitative determination.

The invention discloses a preparation method of a nonionic Trimeric surfactant for efficient wafer cleaning, and the specific preparation method comprises the following steps: S1, mixing polyethylene glycol monomethyl ether and BOC-tertiary leucine, adding dichloromethane as a reaction solvent, adding a catalyst and an activator, reacting at room temperature for 20-30 hours, and collecting a crude product BOC-tertiary leucine ester; s2, extracting the crude product BOC-tertiary leucine ester obtained in the step S1 with deionized water, performing rotary evaporation drying, and performing column chromatography purification to obtain purified BOC-tertiary leucine ester; s3, mixing the BOC-tert-leucine ester purified in the step S2 with dichloromethane, dropwise adding trifluoroacetic acid at 0 DEG C, gradually heating to room temperature, and stirring to react for 2-4 hours to obtain a crude product tert-leucine ester; s4, adjusting the pH value of the crude product tert-leucine ester to 8-9 by using a NaOH aqueous solution with the concentration of 1 mol / L, extracting, and carrying out rotary evaporation drying to obtain tert-leucine ester; and S5, adding the tertiary leucine ester in the step S4 and trimesic aldehyde into an ethanol solution, mixing, carrying out reflux reaction for 10-15 hours at 78-80 DEG C, and carrying out rotary evaporation drying and column chromatography purification to obtain the nonionic Trimeric surfactant.

The application relates to an application method of L-tert-leucine for improving the quality and storability of Gala apples, which comprises the following steps: selecting a test apple orchard base, selecting Gala apple trees, and spraying different concentrations of exogenous L-tert-leucine on the selected test apple trees in a fruit development period; picking 8-9 mature Gala fruits, storing the fruits, and comparing the fruit quality indexes of different groups and comparing various measured data. According to the application method, spraying 500 mg / L mass concentration L-tert-leucine on the leaves of Gala apples in a growth period can significantly promote the yellow coloration and brightness increase of the Gala apples at the time of harvesting, improve the fruit hardness, TSS content, TA content, soluble protein content and free amino acid content, is beneficial to maintaining the fruit hardness and soluble protein content during cold storage, delaying the decrease of the fruit hardness and soluble protein content, inhibiting the decrease of the TSS content and TA content and the increase of the free amino acid during cold storage, and keeping the Gala apple fruits with lower POD activity and higher SOD activity.

The invention discloses a preparation method and application of tert-butyl peptide, and relates to the field of agricultural biology.The preparation method comprises the following steps that S1, L-tertiary leucine and ethylene glycol are mixed and react for 10-14 hours at the temperature of 180-200 DEG C under the reflux condition; and S2, after the reaction is finished, cooling to separate out a solid, and washing and drying to obtain the tert-butyl peptide, according to the preparation method and application of the tert-butyl peptide, L-tert-leucine and ethylene glycol which are low in price and easy to obtain serve as raw materials, the tert-butyl peptide can be prepared through one-step reaction, conditions are mild, the yield is high, and the preparation method is suitable for large-scale preparation; the tert-butyl peptide provided by the invention is a novel compound which is not reported, and experiments show that the tert-butyl peptide shows excellent biological activity at extremely low concentration; compared with a known regulator, the tert-butyl peptide disclosed by the invention shows a full-chain and systematic improvement effect on nitrogen metabolism of various crops at an extremely low concentration.

The application provides a process for preparing L-tert-leucine by an enzymatic method. The application first performs carboxyl group treatment on graphene oxide, promotes the combination of the graphene oxide with a phenolic aldehyde prepolymer by increasing surface active sites, forms graphene oxide-phenolic aldehyde copolymer, and then loads gold nanoparticles by a light deposition method to prepare a modified phenolic aldehyde photocatalyst. In the catalyst, the quinone-type-benzene type units formed by the polycondensation of the phenolic aldehyde structure constitute an electronic D-A conjugate pair, the plasmonic resonance effect of the gold nanoparticles can effectively broaden the light absorption range, enrich the photo-generated electrons and accelerate the carrier separation, and the NADH regeneration efficiency is significantly improved; meanwhile, the photocatalytic activity of the graphene oxide and the two-dimensional sheet layer and the surface functional groups thereof can optimize the internal electron transport path of the catalyst, and synergistically enhance the electron transfer efficiency of the system with other components. The above modification strategy builds an efficient coenzyme regeneration system through the synergistic effect of multiple components, and realizes the high substrate conversion rate and high light purity preparation of L-tert-leucine.