28 results about "Small peptide" patented technology

The application discloses application and a method of a small peptide GLP-1(28-36)amide in preparation of a medicine for preventing and treating rice blast. A scheme discloses application of the small peptide GLP-1(28-36)amide in preparation of a medicine for preventing and treating Pyricularia oryzae, and application of the provided small peptide GLP-1(28-36)amide in inhibiting Pyricularia oryzae and preventing and treating rice blast. The small peptide GLP-1(28-36)amide of the application can effectively inhibit pathogenicity of spores of Pyricularia oryzae in a concentration range of 12.5-100 muM, in particular, the small peptide GLP-1(28-36)amide with a concentration of 100 muM can completely prevent and treat rice blast, and can be used for developing a new type of green pesticide.

The application belongs to the technical field of genetic engineering, and particularly relates to a soybean unconventional small peptide miPEP160b, a gene and application thereof. The application provides a soybean unconventional small peptide miPEP160b, and an amino acid sequence of the soybean unconventional small peptide miPEP160b is shown as SEQ ID NO. 2. The soybean unconventional small peptide miPEP160b can regulate symbiotic nodule formation of legume plants, can be used as a gene resource for biological breeding, and can be used for cultivating new varieties of legume plants with improved nodule formation ability and nitrogen fixation efficiency by using biological materials related to the soybean unconventional small peptide miPEP160b, thereby providing a new idea for soybean biological breeding and agricultural production application. In addition, the soybean unconventional small peptide miPEP160b can be used as a plant stimulant, can promote nodule formation ability and nitrogen fixation efficiency of soybeans by spraying around the roots of drip irrigation soybeans, and can also promote growth and development of soybean plants.

The application belongs to the technical field of cottonseed meal processing and animal feed, and particularly relates to application of enzymatic cottonseed meal in preparation of low-protein daily ration, promotion of animal intestinal development, improvement of animal production performance and improvement of carcass quality, wherein the small peptide content, free amino acid content and antioxidant activity of the enzymatic cottonseed meal are all significantly improved compared with cottonseed meal, the change of protein secondary structure and the significant reduction of relative molecular weight are more conducive to promotion of animal absorption of small molecules, are beneficial to animal intestinal health development, and can effectively improve animal growth performance. The feed prepared by replacing part of the conventional feed with the enzymatic cottonseed meal can still achieve the effects of promoting animal intestinal development and improving animal growth performance under the condition that the crude protein content is reduced by 1% to 2% compared with the basic daily ration or the conventional feed, and the feeding cost is significantly reduced compared with the use of the conventional feed.

This invention discloses a method for preparing and applying plant sulfonated small peptide PSK based on synthetic biology, belonging to the field of biotechnology. Based on the design concept of synthetic biology, this invention constructs an orthogonal synthetic system composed of genetic code expansion technology and SUMO fusion protease digestion technology. A specific PSK dotted vector and a pUltra-sY vector are co-transformed into *E. coli* to construct a genetically engineered strain. The sulfonated small peptide PSK precursor is expressed in this genetically engineered strain, and mature plant sulfonated small peptide PSK is obtained through specific hydrolysis by SUMO protease. This method significantly improves the synthesis efficiency and purity of sulfonated small peptides and has broad application prospects in agricultural production and biotechnology.

This invention discloses the application of the CLE11 small peptide gene in regulating plant salt tolerance, belonging to the field of biomaterials technology. This invention uses millet as the research material and clones... SiPROCLE11 Gene (CLE11 small peptide gene), construction SiPROCLE11 Overexpression and silencing of millet materials, exogenous application of the synthetic peptide SiCLE11p, and its role in enhancing millet salt tolerance were determined. Further investigation revealed that it can promote lateral root development and reduce Na+ in the roots. + This accumulation of knowledge laid the foundation for the discovery of salt-tolerant genes in millet and for salt-tolerant millet breeding.

The present application aims to provide a preparation method of rice bran fermented peptide for goose feed, which comprises the following steps: fermenting fresh rice bran to obtain an enzymatic fermentation compound, and feeding the goose with the enzymatic fermentation compound, so that the small peptides and intestinal beneficial bacteria produced can provide better nutrition, are easy to absorb, improve the disease resistance rate, and can avoid oxidation of the rice bran and prolong the storage period of the rice bran.

The application discloses a lincRNA small peptide coding potential identification method and system, and belongs to the technical field of computer technology and bioinformatics. The method first processes lincRNA sequences into a standardized sequence set; then identifies and integrates potential coding fragments to obtain a candidate coding region set; after extracting the multi-dimensional feature codes of each region, the fusion prediction confidence and label are obtained through the Mamba model and the machine learning model; finally, the coding potential is determined, and if there is no coding potential, the conclusion is output, and if there is coding potential, the amino acid sequence is deduced, the comprehensive ranking confidence is calculated and ranked by combining multiple indexes, and the high and low confidence small peptide sets are screened according to the threshold. The application introduces an efficient sequence state space modeling structure, does not need to rely on ribosome sequencing or mass spectrometry data, realizes high-precision, low-complexity and scalable prediction of the potential small peptide coding capacity in the lincRNA sequence, and solves the technical defects that the existing methods are difficult to balance between accuracy and computational efficiency.

This invention discloses the Anti-WOX11 small peptide from Aquilaria sinensis and its application in promoting plant root regeneration. Through genetic transformation, this invention obtained transgenic Aquilaria sinensis and Arabidopsis thaliana containing the Anti-WOX11 fragment. Experiments demonstrated that Anti-WOX11 can promote the regeneration of plant cuttings and the development of lateral roots. This invention fills the research gap on the direct regulation of cutting regeneration by small peptides in Aquilaria sinensis, providing a feasible solution for improving the survival rate of Aquilaria sinensis after root pruning and transplanting. It has great research potential and broad application prospects, and can be applied to promoting agarwood formation and the production and preparation of agarwood-related products.

The application discloses a kind of complex small molecule coral food and its application in adjusting coral symbiotic microbial community structure.The raw materials of the complex small molecule coral food include: soybean meal small peptide protein, cod small peptide protein, haematococcus pluvialis powder, complex amino acid, squid meal, chitosan oligosaccharide and complex nucleotide.The complex small molecule coral food of the application takes soybean meal small peptide protein and cod small peptide protein as main component, haematococcus pluvialis as secondary component, and is supplemented with complex amino acid and squid meal, and is additionally added with chitosan oligosaccharide and complex nucleotide.The obtained complex small molecule coral food not only has good palatability, but also ensures the feeding amount of coral.It is verified by test that the complex small molecule coral food of the application can adjust the symbiotic microbial community structure of aquarium coral, improve the proportion of coral beneficial symbiotic bacteria, and further improve the environmental tolerance of coral.

Methods and materials for preventing and modulating atherosclerosis. In particular, small peptides that are capable of interacting with CD40, thereby interfering with the ability of CD40 to interact with CD154, which impacts inflammation and atherosclerosis. The use of such peptides in reducing atherosclerosis, and in particular, the autoimmune inflammatory response that may be a driving factor thereof. The use of such short peptides to lower LDL cholesterol. Methods and materials for detecting T-cells that express CD40 (Th40 cells). Methods and materials for preventing, modulating, reducing and / or reversing type 2 diabetes and auto-inflammatory disease. In particular, small peptides that are capable of interacting with CD40, thereby interfering with the ability of CD40 to interact with CD154, which impacts inflammation and type 2 diabetes. The use of such peptides in reducing type 2 diabetes, and in particular, the autoimmune inflammatory response that may be a driving factor thereof. The use of such short peptides to lower IL2, INFγ, and IL17a. The use of such peptides to increase glucose transport protein (GLUT4). Methods and materials for detecting T-cells that express CD40 (Th40 cells).

The application belongs to the field of molecular biological technology, and specifically discloses application of a small peptide miPEP171b in improving cold resistance of grape. The miPEP171b is derived from grape, and the amino acid sequence is MKGPGGKAGHLIERPSV*. The application proves that the miPEP171b has translation activity, is induced to express under low temperature, can regulate transcription of pri-miR171b in a positive direction, promote accumulation of mature vvi-miR171, and inhibit expression of a target gene VvSCL15 / VvSCL27, effectively improves cold resistance of grape, and is helpful to deal with disastrous weather such as "early spring cold". The application provides two methods of improving cold resistance of grape, i.e. exogenous application of the miPEP171b small peptide and overexpression of a coding gene of the miPEP171b, and provides a brand-new molecular target and application strategy for crop stress resistance breeding and stress resistance cultivation.

This application relates to site-specific conjugation of antibody lysine residues using solid phase immobilized microbial transglutaminase MTG and MTG in solution. Site-specific modification of proteins using microbial transglutaminase (MTG) is a powerful and versatile strategy for controlled modification of proteins under physiological conditions. We present evidence that solid phase microbead immobilization can be used to site-specifically and efficiently link different functional molecules important for further downstream applications to therapeutically relevant proteins, including scFV, Fab fragments and antibodies. We demonstrate that MTG remains firmly immobilized and there is no detectable column bleed and the enzyme activity is maintained during continuous operation, which allows for convenient recycling of the enzyme, thus outperforming solution phase MTG conjugation. Furthermore, it is shown that immobilized MTG exhibits an enhanced selectivity for certain residues in the presence of several reactive residues, all of which are targeted if conjugation is performed in solution. Site-specific lysine conjugation of antibodies using immobilized and solution MTG with a highly efficient glutamine-containing peptide is also reported. In addition, the generation of bis-site-specific conjugated IgGl with site-specific conjugation of both glutamine and lysine residues of an IgGl antibody using immobilized and solution MTG is reported. Site-specific glutamine conjugation with small peptides containing lysine residues and functional moieties is also described.

The application relates to the technical field of animal feed processing, and discloses a pork pig fattening feed based on composite microbial fermentation and application thereof, wherein the feed comprises basic feed and nutrient-loaded biochar; the biochar is loaded with amino acids and trace elements and has pH response characteristics: stable adsorption in a neutral environment with pH 6.0-7.0, and release in an acidic environment with pH 4.0-5.0. The application also provides a three-stage fermentation process, through second-stage lactic acid bacteria acidification, triggering the biochar to release nutrients in the third stage (spore bacillus proliferation stage). The application realizes timed supply of nutrients, significantly improves the enzyme activity, small peptide content and spore bacillus viable count of the feed, and shows lower feed-meat ratio and diarrhea rate in animal feeding.

Disclosed herein are small anti-inflammatory peptides for blocking NF-κB. The peptides and compositions including the peptides can be administered to inhibit cytokine production and / or to inhibit lung inflammation in individuals in need thereof. Particular embodiments include administration to individuals suffering from coronavirus disease 2019 (COVID-19).

This application discloses an immunoassay kit, its preparation method, and its application. The immunoassay kit includes a small peptide-coated solid-phase carrier, a bispecific antibody, and a labeled detection antibody. The bispecific antibody includes an anti-small peptide antibody and a linked capture antibody. The anti-small peptide antibody specifically binds to the small peptide, and both the capture antibody and the labeled detection antibody specifically bind to the target analyte and have different antigen recognition epitopes. The highly specific binding of the small peptide to the anti-small peptide antibody achieves efficient immobilization of the bispecific antibody, significantly improving antibody immobilization density and activity retention compared to traditional detection methods. The targeted binding effect of the bispecific antibody amplifies the detection signal while avoiding competitive interference from endogenous biotin, achieving highly sensitive and interference-resistant immunoassay.

The application belongs to the technical field of biological fermentation, and discloses a method for efficiently preparing earthworm functional liquid by using earthworm intestinal endogenous probiotics and enzymes and the earthworm functional liquid. The method comprises the following steps: step 1: activating and expanding B. velezensis XY1, P. megaspora and Lactococcus lactis respectively to obtain B. velezensis XY1 bacterial liquid, P. megaspora bacterial liquid and Lactococcus lactis bacterial liquid; and step 2: inoculating the B. velezensis XY1 bacterial liquid, the P. megaspora bacterial liquid, the Lactococcus lactis bacterial liquid and a compound enzyme into earthworm slurry to obtain the earthworm functional liquid. The B. velezensis XY1, the P. megaspora, the Lactococcus lactis and the compound enzyme are used for synergistic fermentation to obtain the earthworm functional liquid, the content of total amino acids and small peptides is significantly improved, and the effect of the earthworm functional liquid can be further improved.

This invention belongs to the field of feed processing technology and provides a compound bacterial seed liquid for solid-state fermentation of small peptide cottonseed meal feed, and a method for preparing small peptide cottonseed meal feed using this seed liquid. This invention uses a mixture of Aspergillus niger, Aspergillus oryzae, and Saccharomyces cerevisiae seed liquids in a volume ratio of 1:3:1, wherein the viable cell concentrations of Aspergillus niger, Aspergillus oryzae, and Saccharomyces cerevisiae are 1.0×10¹¹ CFU / mL, 1.1×10¹¹ CFU / mL, and 1.2×10¹¹ CFU / mL, respectively. Cottonseed meal is used as the substrate, with the addition of ammonium chloride and potassium dihydrogen phosphate, and fermented under constant temperature. The fermented cottonseed meal prepared by this invention is characterized by high protein, high small peptide content, and zero gossypol, which can significantly improve animal growth performance and provide a feasible solution for the high-value application of cottonseed meal in the feed industry.

Enzymatic Preparation and Immunological Application of Small Molecule Turtle Peptides. The method for preparing turtle peptides includes: providing turtle protein raw materials; performing a primary enzymatic hydrolysis of the turtle protein raw materials using an alkaline protease; performing a secondary enzymatic hydrolysis of the primary hydrolysis product using a complex enzyme containing flavor protease and trypsin; and inactivating, separating, and purifying the secondary hydrolysis product to obtain turtle peptides. This invention efficiently degrades large molecule turtle protein into small peptides and oligopeptides with an average molecular weight of less than 1000 Da, especially 180-800 Da, through stepwise synergistic enzymatic hydrolysis. These small peptides can be directly and completely absorbed through the intestinal peptide transport system, with an absorption rate and efficiency far exceeding that of free amino acids and whole proteins, significantly improving bioavailability. The small molecule turtle peptides prepared by this invention can significantly enhance the phagocytic capacity of macrophages and promote the proliferation and differentiation of lymphocytes, thereby enhancing the body's immune function at both the innate and adaptive immune levels.

This invention discloses a bio-organic fertilizer, its preparation method, and its application, belonging to the field of bio-organic fertilizer technology. The invention separates dead silkworms and / or silkworm moths into two parts. The first part undergoes preservation, sterilization, and modification treatments, causing partial hydrolysis of proteins into small peptides and free amino acids, and degradation of chitin into chitosan oligosaccharides, yielding modified silkworm body material. The second part undergoes carbonization treatment to obtain silkworm biochar. The modified silkworm body material, silkworm biochar, and silkworm excrement are compounded in a weight ratio of (10-40):(10-40):(50-80), inoculated with a compound microbial agent, and subjected to aerobic fermentation to obtain the bio-organic fertilizer. This invention achieves the synergistic utilization of dead silkworms, silkworm moths, and silkworm excrement for the first time, overcoming the technical prejudice that dead silkworms "can only be discarded." The product has a total nitrogen content of 5%-7%, possessing both a readily available nitrogen source and the plant immune-inducing function of chitosan oligosaccharides, realizing the complete closed-loop utilization of silkworm breeding waste. This invention can be widely applied to the planting of mulberry trees, rice, rapeseed, corn, wheat, sugarcane, beans, and other cash crops.

The application discloses a kind of SuSy2-SUT4-SWEET5 gene tandem modules of Liangshan dendrocalamus, comprising: promoter 35S, DfSuSy2 gene, 3×Flag label, self-cleavage small peptide P2A, DfSWEET5 gene, 3×HA label, DfSUT4 gene and EGFP label;The permutation and combination is 35S::DfSUSy2-3×Flag-P2A-DfSWEET5-3×HA-P2A-DfSUT4-EGFP.The SuSy2-SUT4-SWEET5 gene tandem module of Liangshan dendrocalamus of the application is related to the whole pathway of regulation bamboo plant sucrose transport, distribution, metabolism, and the above three linear processes are jointly studied, i.e., tandem co-expression, can dose-increased cellulose content in plant, and then cultivate high-fiber content, high biomass excellent plant resources, which has far-reaching significance for improving, application, popularization and promotion of fiber bamboo.

The application discloses a Rosa chinensis organ development regulation gene, a small peptide thereof and application, and belongs to the technical field of plant genetic engineering.The application provides a kind of gene RwCLV3 for regulating the development of Rosa chinensis flower organ, and the nucleotide sequence is shown as SEQ ID NO.1;The application effectively regulates the development of Rosa chinensis and Arabidopsis thaliana flower organ by means of genetic engineering, can significantly change the size of apical meristem and the development and quantity of flower organ.The application provides a new gene resource for improving plant flower organ development and improving ornamental.

A pharmaceutical composition for boosting an immune response contains TREM-like transcript-1 (TREML1) extracellular domain (ECD) or stalk polypeptide. The TREML1 ECD or stalk polypeptide is derived from human or mouse TREML1. The pharmaceutical composition further contains an antigen as a vaccine, wherein the TREML1 ECD or stalk polypeptide functions as an adjuvant or immune booster.

The application discloses a method for promoting adventitious shoot regeneration of sunflower in vitro, and belongs to the technical field of plant tissue culture. The method comprises the following steps: taking sunflower 'YK86' sterile seedling cotyledon as an explant, inoculating the explant into a callus induction medium containing 0.01 nM exogenous HaREF1 small peptide and a bud differentiation medium in sequence after pretreatment, effectively promoting sunflower callus induction and adventitious shoot differentiation and regeneration, reducing the browning rate, and successfully inducing adventitious shoots. Through screening of the genotype and explant of sunflower in vitro regeneration, in combination with the synergistic effect of screening and adding different concentrations of HaREF1 to the callus induction medium and the bud differentiation medium, the application effectively promotes the differentiation and regeneration of sunflower adventitious shoots in vitro, solves the problems of difficult sunflower in vitro regeneration, low regeneration efficiency and instability, and provides technical support for sunflower genetic transformation, gene function verification and variety improvement.

The application discloses application of CLE small peptides in regulation of peach adventitious root regeneration. Group-A CLE small peptides in the application can promote peach adventitious root regeneration, and specifically, PpCLE4 small peptides, PpCLE5 small peptides and PpCLE7. After the peach stem segments of the PpCLE4 small peptides, the PpCLE5 small peptides and the PpCLE7 are used as explants, the adventitious root regeneration efficiency is respectively increased to 69.70%, 43.33% and 38.79%, and the number of adventitious roots regenerated from a single explant is respectively increased to 2.40, 2.63 and 2.58, which is much better than that of a control group without adding CLE small peptides and a treatment group with other types of CLE small peptides. The Group-A CLE small peptides provided by the application have good research potential and wide application prospect, and provide a new method for promoting adventitious root regeneration of peach cutting rooting.

The application discloses a peanut meal fermentation process by using composite bacteria and a fermentation product thereof, and belongs to the technical field of peanut meal fermentation. The process comprises the following steps: fermenting peanut meal mixture by using composite bacteria; the composite bacteria are bacillus velezensis, bacillus amyloliquefaciens, pediococcus acidilactici and lactobacillus rhamnosus; the composite bacteria are prepared into composite bacteria liquid before fermentation; the composite bacteria liquid is composed of bacillus velezensis liquid, bacillus amyloliquefaciens liquid, pediococcus acidilactici liquid and lactobacillus rhamnosus liquid. By adopting the co-fermentation system of bacillus and lactic acid bacteria, the functions of enzymolysis, detoxification, pH adjustment and bacteriostasis are synergized, the retention rate of crude protein in the fermentation product is high, the contents of acid-soluble protein and small peptides are greatly increased, the removal effect of aflatoxin is remarkable, the pH is reduced to the acidic range, and the biological safety is improved.

The application discloses application of a RALF small peptide in improving the hardness of strawberry fruits, and the RALF small peptide is a FvRALF5 mature peptide or a FvRALF6 mature peptide. The application can significantly improve the hardness of strawberry fruits by injecting the RALF small peptide, and then delay the softening process, which is helpful to prolong the shelf life of the strawberry fruits after harvesting and reduce postharvest loss.