171 results about "Small peptide" patented technology

The invention provides copper-loaded duck-origin lactobacillus plantarum with high antibacterial peptide yield and application of the copper-loaded duck-origin lactobacillus plantarum. The preservation number of the provided copper-loaded duck-origin lactobacillus plantarum is CGMCC (China General Microbiological Culture Collection Center) NO.32835. The domesticated and bred duck-origin lactobacillus plantarum with high antibacterial peptide yield can tolerate high-concentration copper ions, the domesticated and bred lactobacillus plantarum can stably grow, and the contents of antibacterial peptide, extracellular protein, lipase, amylase, lactic acid, acetic acid and the like in the domesticated and bred lactobacillus plantarum strain are increased; and the amino acid composition is changed. The strain can convert inorganic copper into organic copper, the utilization rate of copper is increased, and growth and development of organisms are promoted. Moreover, small peptides and extracellular proteins in metabiotics generated after fermentation have the effect of effectively inhibiting harmful bacteria such as riemerella anatipestifer, escherichia coli, salmonella and staphylococcus of poultry, and are beneficial to eliminating intestinal inflammatory factors, maintaining intestinal health and improving the feed utilization rate.

The invention belongs to the field of corn disease prevention, relates to a novel small peptide, provides a small peptide NCRP246 and application thereof in improvement of corn southern leaf blight resistance, and provides feasibility for plant disease resistance by using non-classical peptides. A corn southern leaf blight resistance identification experiment proves that NCRP246 can significantly improve the resistance of corn to southern leaf blight, and further researches that the NCRP246 reduces oxidative damage of pathogenic bacteria infection of the southern leaf blight to the corn by reducing H2O2 accumulation so as to mediate the resistance of the corn to the southern leaf blight; the analysis from the molecular perspective proves that the NCRP246 can induce the expression of defense related genes.

The invention relates to the field of agricultural products, and particularly discloses a polypeptide for improving plant disease resistance, and the polypeptide is LAND2 small peptide and homologous peptide thereof. The LAND2 small peptide disclosed by the invention is screened and found from an arabidopsis thaliana proteome, and homologous peptides of the LAND2 small peptide are respectively derived from chilies and cucumbers. Tests prove that when the LAND2 small peptide is externally applied to arabidopsis thaliana, the local and systematic resistance of plants to botrytis cinerea can be enhanced, and the LAND2 small peptide has broad-spectrum immunocompetence in chilies and cucumbers. The polypeptide is practically applied to agricultural production, can enhance the disease resistance of plants and improve the yield and quality of the plants, and has practical popularization and application values.

The invention discloses an antibacterial small peptide CsCAPE9 and application of the antibacterial small peptide CsCAPE9 in prevention and control of citrus huanglongbing. The amino acid sequence of the antibacterial small peptide CsCAPE9 is as shown in SEQ ID NO.1; the antibacterial small peptide CsCAPE9 provided by the invention is a small peptide synthesized in a plant body, and the antibacterial small peptide CsCAPE9 can activate biosynthesis of salicylic acid and induce systematic defense reaction of citrus. The antibacterial small peptide CsCAPE9 has a good inhibition effect on pathogenic bacteria of citrus huanglongbing in greenhouses and fields, and has a wide application prospect in comprehensive prevention and control of the huanglongbing.

The invention discloses saccharomyces boulardii, a culture and a preparation method and application of the saccharomyces boulardii. The preservation number of the strain is CCTCC (China Center for Type Culture Collection) NO: M 20252633. The preparation method comprises the following steps: preparing saccharified liquid by taking white spirit vinasse or brewer's grains as a main raw material and adopting a high-temperature alkali treatment and composite enzymolysis process, inoculating strains for liquid-state high-density fermentation, carrying out solid-liquid separation on fermentation liquor, taking a liquid phase, and carrying out spray drying. According to the method, high-value utilization of the waste vinasse is realized, and the production cost is remarkably reduced. The obtained culture is rich in viable bacteria and active small peptides, and has the characteristics of strong tolerance, wide antibacterial spectrum and the like. As a feed additive, the feed additive can effectively reduce the diarrhea rate of piglets, inhibit clostridium perfringens and prawn vibrios of broilers, and improve the oxidation resistance and production performance of animals.

The invention provides a peptide-enriched organic fertilizer as well as a preparation method and application thereof, and belongs to the technical field of organic fertilizer preparation. The preparation method of the peptide-enriched organic fertilizer provided by the invention comprises the following steps: mixing corn soaking water with aspergillus oryzae seed koji, and fermenting for the first time to obtain the peptide-enriched organic fertilizer; the aspergillus oryzae mold starter is obtained by inoculating aspergillus oryzae YY-21 into a culture medium and fermenting 2. According to the preparation method provided by the invention, the peptide-enriched organic fertilizer with high amino acid nitrogen content, high small peptide content, high soluble protein content and high thallus biomass can be prepared, and when the peptide-enriched organic fertilizer is used for planting lettuce, the plant height, the fresh weight of a single plant, the root length, the leaf number and the leaf area of the lettuce can be remarkably increased; the chlorophyll content, the vitamin C content and the total sugar content in the lettuce can be remarkably improved, and the nitrate content of lettuce leaves can be reduced.

The invention discloses a plant endogenous small molecule polypeptide and application thereof in inhibiting RNA virus infected plants, the sequence of the small molecule polypeptide is Pep1-3: IIGVSNFDPVKRS, and the small molecule polypeptide is derived from nicotiana benthamiana; the invention relates to application of plant endogenous small molecule polypeptide in inhibiting RNA virus infection of plants. The plant is tobacco; the RNA viruses comprise tobacco mosaic viruses, tomato mosaic viruses and turnip mosaic viruses, and the small peptide Pep1-3 serves as a novel plant immunity inducer and shows the potential of the small peptide Pep1-3 in the aspect of preventing and controlling the RNA viruses. In agricultural production, prevention and treatment of virus diseases by adopting oligopeptide substances are a green and sustainable direction, and compared with traditional pesticides and plant immunity inducers, the composition has the advantages of broad spectrum, small toxicity, no drug resistance risk and long lasting period, and can generate a synergistic or synergistic effect when being mixed with other medicaments.

The disclosure provides an immunomodulatory octapeptide, and this small peptide molecule has a unique structure, higher broad-spectrum antibacterial activity, stronger pathogenic bacteria inhibition and high purity, can maintain stability of its structure and antibacterial activity in artificial gastric juice, with no obvious hemolytic activity, and has high application value in animal husbandry. The disclosure provides a preparation method and a purity test method of this immunomodulatory octapeptide.

The invention discloses an application of a small peptide GLP-1 (28-36) amide in preparation of a medicine for preventing and treating rice blast and a method of the small peptide GLP-1 (28-36) amide. The invention discloses an application of the small peptide GLP-1 (28-36) amide in preparation of a medicine for preventing and treating magnaporthe oryzae, and an application of the small peptide GLP-1 (28-36) amide in inhibition of magnaporthe oryzae and prevention and treatment of magnaporthe oryzae. The small peptide GLP-1 (28-36) amide can effectively inhibit pathogenicity of pyricularia oryzae spores in the concentration range of 12.5-100 [mu] M, especially the small peptide GLP-1 (28-36) amide with the concentration of 100 [mu] M can completely prevent and treat pyricularia oryzae, and the small peptide GLP-1 (28-36) amide can be used for developing novel green pesticides.

The invention discloses a nucleic acid aptamer with enhanced affinity after cyclic hybridization as well as a screening method and application thereof, relates to the technical field of nucleic acid aptamer screening, and successfully develops a CH-SELEX method by taking human alpha-thrombin as a research object on the basis of compatibility with MB-SELEX under the condition of introducing cyclic hybridization. Through multiple rounds of positive screening under the condition of introducing cyclic hybridization and negative screening under the condition of adding His-small peptide modified magnetic beads and human serum, the nucleic acid aptamer which can specifically recognize human alpha-thrombin and has obviously improved affinity after cyclic hybridization is screened; and the affinity of the nucleic acid aptamer can be regulated and controlled by increasing the length of the hybrid complementary chain, so that reference is provided for diagnosis and treatment of diseases related to human alpha-thrombin and development of sensors, concept verification and methodological reference is provided for regulation and control of other important biological processes, and the blank of the method is filled.

The application discloses application and a method of a small peptide GLP-1(28-36)amide in preparation of a medicine for preventing and treating rice blast. A scheme discloses application of the small peptide GLP-1(28-36)amide in preparation of a medicine for preventing and treating Pyricularia oryzae, and application of the provided small peptide GLP-1(28-36)amide in inhibiting Pyricularia oryzae and preventing and treating rice blast. The small peptide GLP-1(28-36)amide of the application can effectively inhibit pathogenicity of spores of Pyricularia oryzae in a concentration range of 12.5-100 muM, in particular, the small peptide GLP-1(28-36)amide with a concentration of 100 muM can completely prevent and treat rice blast, and can be used for developing a new type of green pesticide.

The application belongs to the technical field of genetic engineering, and particularly relates to a soybean unconventional small peptide miPEP160b, a gene and application thereof. The application provides a soybean unconventional small peptide miPEP160b, and an amino acid sequence of the soybean unconventional small peptide miPEP160b is shown as SEQ ID NO. 2. The soybean unconventional small peptide miPEP160b can regulate symbiotic nodule formation of legume plants, can be used as a gene resource for biological breeding, and can be used for cultivating new varieties of legume plants with improved nodule formation ability and nitrogen fixation efficiency by using biological materials related to the soybean unconventional small peptide miPEP160b, thereby providing a new idea for soybean biological breeding and agricultural production application. In addition, the soybean unconventional small peptide miPEP160b can be used as a plant stimulant, can promote nodule formation ability and nitrogen fixation efficiency of soybeans by spraying around the roots of drip irrigation soybeans, and can also promote growth and development of soybean plants.

Disclosed in the present invention are a mitochondrial-localized small peptide (MLSP) and the use thereof in improving the plant yield and / or stress resistance. By means of overexpressing the mitochondrial-localized small peptide (MLSP) in plants, the present invention improves the stability and activity of the mitochondrial respiratory chain in plants and improves the activity of plant mitochondrial complexes, thus improving the plant yield and resistance to drought and saline-alkali stress.

The invention discloses a high-emulsification-activity gluten protein based on irradiation modification and a preparation method thereof in the field of wheat protein processing, and the method comprises the following steps: dispersing wheat gluten protein in deionized water, stirring to form a uniform suspension, adjusting the pH value, carrying out enzymolysis treatment on the suspension by using alkaline protease, and drying to obtain the high-emulsification-activity gluten protein. Centrifugally drying to obtain primary protein powder; dissolving the protein primary powder in an irradiation solvent to obtain a protein dispersion liquid, and irradiating the protein dispersion liquid by using cobalt-60 gamma rays as an irradiation source in an intermittent manner; and taking the irradiated protein dispersion liquid, and performing centrifugal drying to obtain the high-emulsification active gluten protein. The method comprises the following steps: firstly, carrying out limited hydrolysis on gluten protein by using protease to generate a smaller peptide fragment, so that the irradiation efficiency and the emulsion stability are improved; by adopting an intermittent irradiation treatment mode, protein aggregation caused by heat effect can be reduced, the emulsifying effect is improved, and the stability and adhesive force of an adhesive can be improved when the emulsion is applied to the adhesive.

The invention discloses application of CLE small peptide in promoting crop growth and development, and belongs to the technical field of bioengineering. Through water culture and soil culture tests, it is found that the growth and development of the tartary buckwheat can be remarkably promoted through exogenous application of the CLE7 small peptide, and the nitrogen absorption capacity of the tartary buckwheat is improved. A foundation is laid for analyzing the effect of the CLE small peptide in crop nitrogen absorption and utilization, and a basis is provided for developing the CLE7 small peptide into a novel polypeptide fertilizer for actual production in the future.

The application belongs to the technical field of cottonseed meal processing and animal feed, and particularly relates to application of enzymatic cottonseed meal in preparation of low-protein daily ration, promotion of animal intestinal development, improvement of animal production performance and improvement of carcass quality, wherein the small peptide content, free amino acid content and antioxidant activity of the enzymatic cottonseed meal are all significantly improved compared with cottonseed meal, the change of protein secondary structure and the significant reduction of relative molecular weight are more conducive to promotion of animal absorption of small molecules, are beneficial to animal intestinal health development, and can effectively improve animal growth performance. The feed prepared by replacing part of the conventional feed with the enzymatic cottonseed meal can still achieve the effects of promoting animal intestinal development and improving animal growth performance under the condition that the crude protein content is reduced by 1% to 2% compared with the basic daily ration or the conventional feed, and the feeding cost is significantly reduced compared with the use of the conventional feed.

The application provides a polypeptide, a plant immune elicitor and application thereof. The polypeptide has 10 or 11 amino acid residues; the amino acid at the first position of the polypeptide is serine, and the amino acids at the 9th and 10th positions are both cysteine; if the polypeptide has 11 amino acid residues, the amino acid at the 11th position of the polypeptide is asparagine. The polypeptide can solve the problem that there is no small peptide secreted by gramineae plants in the prior art, and is suitable for the field of plant immune elicitors.

The invention provides an antibacterial peptide prepared by utilizing copper-loaded lactobacillus plantarum, which is prepared by inoculating high-copper-loaded duck-origin lactobacillus plantarum into a culture medium for fermentation and then using fermentation liquor, and the preservation number of the copper-loaded duck-origin lactobacillus plantarum is CGMCC (China General Microbiological Culture Collection Center) NO.32835. The lactobacillus plantarum obtained through domestication can tolerate high-concentration copper ions, the lactobacillus plantarum obtained through domestication and breeding can stably grow, and metabiotics generated through fermentation of the lactobacillus plantarum contain small peptides, small molecular organic acids, digestive enzymes and the like. Due to the fact that the antibiotic peptide rich in the afterbiogen has an obvious inhibiting effect on harmful bacteria of waterfowls, the prepared antibiotic peptide preparation effectively improves the antibacterial activity, and as a feed additive, the antibiotic peptide preparation is beneficial to elimination of inflammatory factors of intestinal tracts of poultry, maintenance of intestinal health, increase of the utilization rate of feed and improvement of the product quality.

The invention discloses a duck-derived flavor peptide enrichment and identification method, and belongs to the technical field of food flavor and biology. The method comprises the following steps: performing after-ripening treatment on pickled duck embryos to enrich small peptides; performing extraction and ultrafiltration to obtain components less than 3kDa; carrying out LC-MS / MS identification on the sequence; using multiple machine learning models to crossly predict the palatable taste potential; carrying out molecular docking simulation on the candidate peptide and an umami receptor T1R1 / T1R3; and synthesizing and carrying out sensory verification. According to the method, six brand-new flavor peptides (SEQ ID NO: 1-6) are identified from after-ripe duck meat for the first time, the flavor threshold value of the six brand-new flavor peptides is 0.09-0.30 mmol / L, and the strength of the six brand-new flavor peptides is superior to that of monosodium glutamate. The method provided by the invention is efficient and accurate, and the obtained umami peptide is natural and safe and has important application value in the fields of food seasoning and flavor enhancement.

This invention discloses a method for preparing and applying plant sulfonated small peptide PSK based on synthetic biology, belonging to the field of biotechnology. Based on the design concept of synthetic biology, this invention constructs an orthogonal synthetic system composed of genetic code expansion technology and SUMO fusion protease digestion technology. A specific PSK dotted vector and a pUltra-sY vector are co-transformed into *E. coli* to construct a genetically engineered strain. The sulfonated small peptide PSK precursor is expressed in this genetically engineered strain, and mature plant sulfonated small peptide PSK is obtained through specific hydrolysis by SUMO protease. This method significantly improves the synthesis efficiency and purity of sulfonated small peptides and has broad application prospects in agricultural production and biotechnology.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to application of a PROSiCAPE14 gene and a protein encoded by the PROSiCAPE14 gene to improvement of plant salt tolerance. According to the invention, it is found for the first time that salt stress can cause down-regulation of the expression of the millet PROSiCAPE14 gene. Under a salt treatment condition, the plant height and the root length of a PROSiCAPE14 gene coded small peptide externally applied to millet and a constructed PROSiCAPE14 gene overexpression strain are obviously worse than those of a control group, and the plant height and the root length of a PROSiCAPE14 gene silent plant are obviously better than those of the control group. It is indicated that the PROSiCAPE14 gene plays an important role in the salt stress response process of the millet, and a foundation is provided for further research on the effect of the CAPE14 gene in the adaptation of the millet to abiotic stress and other possible biological functions.

The invention belongs to the field of feed protein processing, and particularly relates to a method for improving the bioavailability of animal feed protein through chemical enzymolysis. The method comprises the following steps: mixing a compound protein raw material with water to form a suspension; sodium tripolyphosphate is added for phosphorylation pretreatment, so that protein is subjected to specific modification; after the pH is adjusted to be acidic, pepsase and acid phosphatase are added at the same time for an enzymolysis reaction; and after the reaction is finished, performing enzyme deactivation and drying to obtain the modified feed protein. According to the method, a specific enzyme cutting site is created on a protein molecule through chemical phosphorylation, then directional hydrolysis is achieved through efficient recognition of pepsin on the site, and meanwhile dynamic regulation and control of the hydrolysis process are achieved by means of the dephosphorylation effect of acid phosphatase. The obtained product has the outstanding advantages of high content of small peptides, good digestive absorbability, low bitterness, strong biological activity and the like, and the bioavailability of feed protein is remarkably improved.

The invention relates to the technical field of biological medicines, in particular to application of canthaxanthin extract and a feed additive containing the canthaxanthin extract in silkworm breeding, and finds that cocoon shell amount, whole cocoon amount and pupa weight of silkworms can be remarkably increased and egg laying amount of single moths can be increased by adding canthaxanthin extract to silkworms for the first time. Meanwhile, the silkworm variety with functional carotenoid binding protein can also be induced to produce orange red natural colored cocoons. The invention further provides a compound feed additive containing the canthaxanthin extract and the sericin-derived small peptide and / or gamma-aminobutyric acid. Experiments prove that the canthaxanthin extract and the sericin-derived small peptide have a synergistic effect in the aspect of increasing the cocoon shell amount. According to the technical scheme, on the premise that normal growth and development of silkworms are not affected, the multiple purposes of yield increasing, propagation promoting and color increasing are achieved at the same time, and the method has good application prospects and industrial value.

The invention relates to an MOTS-c targeted delivery system based on an exosome derived from astragalus membranaceus and application of the MOTS-c targeted delivery system in myocardial ischemia-reperfusion injury. The preparation method comprises the following steps: preparing an exosome AExo from radix astragali from fresh radix astragali as a raw material, modifying a cardiac troponin I targeting peptide LPFFD to the surface of the AExo by using an EDC / NHS chemical cross-linking method to obtain LPFFD-AExo, and loading a mitochondrial derived small peptide MOTS-c into the LPFFD-AExo by using an ultrasonic-assisted penetration method to obtain the LPFFD-AExo-MOTS-c. According to the invention, the accurate delivery efficiency of the damaged myocardial tissue can be improved, and efficient and accurate delivery of MOTS-c to the ischemia reperfusion damaged myocardial tissue is realized, so that the ferroptosis inhibition capability of MOTS-c is obviously enhanced, the myocardial protection effect on MI / R injury is improved, and finally efficient targeted therapy on MI / R injury is realized.

This invention discloses the application of the CLE11 small peptide gene in regulating plant salt tolerance, belonging to the field of biomaterials technology. This invention uses millet as the research material and clones... SiPROCLE11 Gene (CLE11 small peptide gene), construction SiPROCLE11 Overexpression and silencing of millet materials, exogenous application of the synthetic peptide SiCLE11p, and its role in enhancing millet salt tolerance were determined. Further investigation revealed that it can promote lateral root development and reduce Na+ in the roots. + This accumulation of knowledge laid the foundation for the discovery of salt-tolerant genes in millet and for salt-tolerant millet breeding.

The present application aims to provide a preparation method of rice bran fermented peptide for goose feed, which comprises the following steps: fermenting fresh rice bran to obtain an enzymatic fermentation compound, and feeding the goose with the enzymatic fermentation compound, so that the small peptides and intestinal beneficial bacteria produced can provide better nutrition, are easy to absorb, improve the disease resistance rate, and can avoid oxidation of the rice bran and prolong the storage period of the rice bran.

The invention provides a gH625-H2A histone as well as chromatin and application thereof. The histone is prepared by adding peptide gH625 into histone H2A; the application comprises the application of the gH625-H2A histone and the chromatin thereof in preparation of drugs or drug delivery systems and the application of the gH625-H2A histone and the chromatin thereof in preparation of skin care products and cosmetics. The invention provides the gH625-H2A histone and the chromatin thereof, the histone and the chromatin have strong cell penetration and skin permeation effects, the histone and the chromatin are expected to promote the development of a transdermal gene delivery system and biological medicines and skin care products, and the prepared biological medicines and the skin care products have the advantages that the development of the transdermal gene delivery system and the biological medicines and the skin care products is facilitated; passive skin delivery of nucleic acids, small peptides, growth factors and enzymes is achieved without physical or chemical intervention.

This invention discloses a kitten food without palatability enhancers and its preparation method, relating to the field of pet food technology. It comprises the following components: polypeptide chicken meal, soy protein peptides, gluten hydrolysate, lactoferrin, astragalus extract, yam powder, rosemary oil, resveratrol, tributyrate, and rice. By adding substances containing small peptides and plant extracts to the kitten food, this invention provides the body with a large amount of high-quality animal protein without adding any chemically synthesized palatability enhancers, preservatives, or other chemical additives. This effectively promotes the body's decomposition and metabolism of nutrients, promotes the growth of kittens, and also enhances the body's antioxidant capacity and reduces the occurrence of diarrhea.

The application discloses a kind of Gui Shiwei Chinese herbal medicine organophosphorus pesticide rapid detection method and detection kit, by the sample of Gui Shiwei medicine powder to be measured is extracted by acetonitrile solution containing acetic acid, salting-out stratification is taken supernatant;With colloidal gold labeled anti-organophosphorus pesticide small peptide antibody incubation, carry out competition immune response in the microwell plate coated with organophosphorus hapten-protein complex;After washing, the absorbance is determined at 450 to 550nm wavelength, calculate competition inhibition rate and compare with preset first threshold value to determine whether it is over standard;The method is simple and rapid;In addition, for the detection needs of different organophosphorus pesticides, by setting different organophosphorus hapten-protein complex coating area in the microwell plate, the simultaneous detection of multiple organophosphorus pesticides is realized, and the applicability of organophosphorus pesticide residue detection is further improved.

The application discloses a small peptide SP07 for regulating drought resistance of crops and application thereof, and the amino acid sequence of the small peptide SP07 is shown as SEQ ID NO. 1. The small peptide SP07 is obtained by a chemical synthesis method, seedling stage corn in drought is treated by spraying, and the survival rate of the corn is significantly improved compared with a control. The small peptide SP07 is sprayed in a field under normal growth and drought environment, the flowering period of the corn is advanced, and the yield-related traits of the corn are significantly improved. In addition, wheat under drought stress can be promoted to flower in advance by spraying the small peptide SP07, and the small peptide SP07 can be used for improving drought resistance of crops.