76 results about "Small peptide" patented technology

The invention discloses saccharomyces boulardii, a culture and a preparation method and application of the saccharomyces boulardii. The preservation number of the strain is CCTCC (China Center for Type Culture Collection) NO: M 20252633. The preparation method comprises the following steps: preparing saccharified liquid by taking white spirit vinasse or brewer's grains as a main raw material and adopting a high-temperature alkali treatment and composite enzymolysis process, inoculating strains for liquid-state high-density fermentation, carrying out solid-liquid separation on fermentation liquor, taking a liquid phase, and carrying out spray drying. According to the method, high-value utilization of the waste vinasse is realized, and the production cost is remarkably reduced. The obtained culture is rich in viable bacteria and active small peptides, and has the characteristics of strong tolerance, wide antibacterial spectrum and the like. As a feed additive, the feed additive can effectively reduce the diarrhea rate of piglets, inhibit clostridium perfringens and prawn vibrios of broilers, and improve the oxidation resistance and production performance of animals.

The disclosure provides an immunomodulatory octapeptide, and this small peptide molecule has a unique structure, higher broad-spectrum antibacterial activity, stronger pathogenic bacteria inhibition and high purity, can maintain stability of its structure and antibacterial activity in artificial gastric juice, with no obvious hemolytic activity, and has high application value in animal husbandry. The disclosure provides a preparation method and a purity test method of this immunomodulatory octapeptide.

The invention discloses an application of a small peptide GLP-1 (28-36) amide in preparation of a medicine for preventing and treating rice blast and a method of the small peptide GLP-1 (28-36) amide. The invention discloses an application of the small peptide GLP-1 (28-36) amide in preparation of a medicine for preventing and treating magnaporthe oryzae, and an application of the small peptide GLP-1 (28-36) amide in inhibition of magnaporthe oryzae and prevention and treatment of magnaporthe oryzae. The small peptide GLP-1 (28-36) amide can effectively inhibit pathogenicity of pyricularia oryzae spores in the concentration range of 12.5-100 [mu] M, especially the small peptide GLP-1 (28-36) amide with the concentration of 100 [mu] M can completely prevent and treat pyricularia oryzae, and the small peptide GLP-1 (28-36) amide can be used for developing novel green pesticides.

The application discloses application and a method of a small peptide GLP-1(28-36)amide in preparation of a medicine for preventing and treating rice blast. A scheme discloses application of the small peptide GLP-1(28-36)amide in preparation of a medicine for preventing and treating Pyricularia oryzae, and application of the provided small peptide GLP-1(28-36)amide in inhibiting Pyricularia oryzae and preventing and treating rice blast. The small peptide GLP-1(28-36)amide of the application can effectively inhibit pathogenicity of spores of Pyricularia oryzae in a concentration range of 12.5-100 muM, in particular, the small peptide GLP-1(28-36)amide with a concentration of 100 muM can completely prevent and treat rice blast, and can be used for developing a new type of green pesticide.

The application belongs to the technical field of genetic engineering, and particularly relates to a soybean unconventional small peptide miPEP160b, a gene and application thereof. The application provides a soybean unconventional small peptide miPEP160b, and an amino acid sequence of the soybean unconventional small peptide miPEP160b is shown as SEQ ID NO. 2. The soybean unconventional small peptide miPEP160b can regulate symbiotic nodule formation of legume plants, can be used as a gene resource for biological breeding, and can be used for cultivating new varieties of legume plants with improved nodule formation ability and nitrogen fixation efficiency by using biological materials related to the soybean unconventional small peptide miPEP160b, thereby providing a new idea for soybean biological breeding and agricultural production application. In addition, the soybean unconventional small peptide miPEP160b can be used as a plant stimulant, can promote nodule formation ability and nitrogen fixation efficiency of soybeans by spraying around the roots of drip irrigation soybeans, and can also promote growth and development of soybean plants.

The invention discloses application of CLE small peptide in promoting crop growth and development, and belongs to the technical field of bioengineering. Through water culture and soil culture tests, it is found that the growth and development of the tartary buckwheat can be remarkably promoted through exogenous application of the CLE7 small peptide, and the nitrogen absorption capacity of the tartary buckwheat is improved. A foundation is laid for analyzing the effect of the CLE small peptide in crop nitrogen absorption and utilization, and a basis is provided for developing the CLE7 small peptide into a novel polypeptide fertilizer for actual production in the future.

The application belongs to the technical field of cottonseed meal processing and animal feed, and particularly relates to application of enzymatic cottonseed meal in preparation of low-protein daily ration, promotion of animal intestinal development, improvement of animal production performance and improvement of carcass quality, wherein the small peptide content, free amino acid content and antioxidant activity of the enzymatic cottonseed meal are all significantly improved compared with cottonseed meal, the change of protein secondary structure and the significant reduction of relative molecular weight are more conducive to promotion of animal absorption of small molecules, are beneficial to animal intestinal health development, and can effectively improve animal growth performance. The feed prepared by replacing part of the conventional feed with the enzymatic cottonseed meal can still achieve the effects of promoting animal intestinal development and improving animal growth performance under the condition that the crude protein content is reduced by 1% to 2% compared with the basic daily ration or the conventional feed, and the feeding cost is significantly reduced compared with the use of the conventional feed.

The invention discloses a duck-derived flavor peptide enrichment and identification method, and belongs to the technical field of food flavor and biology. The method comprises the following steps: performing after-ripening treatment on pickled duck embryos to enrich small peptides; performing extraction and ultrafiltration to obtain components less than 3kDa; carrying out LC-MS / MS identification on the sequence; using multiple machine learning models to crossly predict the palatable taste potential; carrying out molecular docking simulation on the candidate peptide and an umami receptor T1R1 / T1R3; and synthesizing and carrying out sensory verification. According to the method, six brand-new flavor peptides (SEQ ID NO: 1-6) are identified from after-ripe duck meat for the first time, the flavor threshold value of the six brand-new flavor peptides is 0.09-0.30 mmol / L, and the strength of the six brand-new flavor peptides is superior to that of monosodium glutamate. The method provided by the invention is efficient and accurate, and the obtained umami peptide is natural and safe and has important application value in the fields of food seasoning and flavor enhancement.

This invention discloses a method for preparing and applying plant sulfonated small peptide PSK based on synthetic biology, belonging to the field of biotechnology. Based on the design concept of synthetic biology, this invention constructs an orthogonal synthetic system composed of genetic code expansion technology and SUMO fusion protease digestion technology. A specific PSK dotted vector and a pUltra-sY vector are co-transformed into *E. coli* to construct a genetically engineered strain. The sulfonated small peptide PSK precursor is expressed in this genetically engineered strain, and mature plant sulfonated small peptide PSK is obtained through specific hydrolysis by SUMO protease. This method significantly improves the synthesis efficiency and purity of sulfonated small peptides and has broad application prospects in agricultural production and biotechnology.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to application of a PROSiCAPE14 gene and a protein encoded by the PROSiCAPE14 gene to improvement of plant salt tolerance. According to the invention, it is found for the first time that salt stress can cause down-regulation of the expression of the millet PROSiCAPE14 gene. Under a salt treatment condition, the plant height and the root length of a PROSiCAPE14 gene coded small peptide externally applied to millet and a constructed PROSiCAPE14 gene overexpression strain are obviously worse than those of a control group, and the plant height and the root length of a PROSiCAPE14 gene silent plant are obviously better than those of the control group. It is indicated that the PROSiCAPE14 gene plays an important role in the salt stress response process of the millet, and a foundation is provided for further research on the effect of the CAPE14 gene in the adaptation of the millet to abiotic stress and other possible biological functions.

The invention relates to the technical field of biological medicines, in particular to application of canthaxanthin extract and a feed additive containing the canthaxanthin extract in silkworm breeding, and finds that cocoon shell amount, whole cocoon amount and pupa weight of silkworms can be remarkably increased and egg laying amount of single moths can be increased by adding canthaxanthin extract to silkworms for the first time. Meanwhile, the silkworm variety with functional carotenoid binding protein can also be induced to produce orange red natural colored cocoons. The invention further provides a compound feed additive containing the canthaxanthin extract and the sericin-derived small peptide and / or gamma-aminobutyric acid. Experiments prove that the canthaxanthin extract and the sericin-derived small peptide have a synergistic effect in the aspect of increasing the cocoon shell amount. According to the technical scheme, on the premise that normal growth and development of silkworms are not affected, the multiple purposes of yield increasing, propagation promoting and color increasing are achieved at the same time, and the method has good application prospects and industrial value.

This invention discloses the application of the CLE11 small peptide gene in regulating plant salt tolerance, belonging to the field of biomaterials technology. This invention uses millet as the research material and clones... SiPROCLE11 Gene (CLE11 small peptide gene), construction SiPROCLE11 Overexpression and silencing of millet materials, exogenous application of the synthetic peptide SiCLE11p, and its role in enhancing millet salt tolerance were determined. Further investigation revealed that it can promote lateral root development and reduce Na+ in the roots. + This accumulation of knowledge laid the foundation for the discovery of salt-tolerant genes in millet and for salt-tolerant millet breeding.

The present application aims to provide a preparation method of rice bran fermented peptide for goose feed, which comprises the following steps: fermenting fresh rice bran to obtain an enzymatic fermentation compound, and feeding the goose with the enzymatic fermentation compound, so that the small peptides and intestinal beneficial bacteria produced can provide better nutrition, are easy to absorb, improve the disease resistance rate, and can avoid oxidation of the rice bran and prolong the storage period of the rice bran.

This invention discloses a kitten food without palatability enhancers and its preparation method, relating to the field of pet food technology. It comprises the following components: polypeptide chicken meal, soy protein peptides, gluten hydrolysate, lactoferrin, astragalus extract, yam powder, rosemary oil, resveratrol, tributyrate, and rice. By adding substances containing small peptides and plant extracts to the kitten food, this invention provides the body with a large amount of high-quality animal protein without adding any chemically synthesized palatability enhancers, preservatives, or other chemical additives. This effectively promotes the body's decomposition and metabolism of nutrients, promotes the growth of kittens, and also enhances the body's antioxidant capacity and reduces the occurrence of diarrhea.

The invention discloses a lincRNA small peptide coding potential identification method and a lincRNA small peptide coding potential identification system, and belongs to the technical field of computer technology and bioinformatics. The method comprises the following steps: firstly, processing a lincRNA sequence into a standardized sequence set; identifying and integrating the deduplicated potential coding fragments to obtain a candidate coding region set; after extracting multi-dimensional feature codes of each region, performing fusion through a Mama model and a machine learning model to obtain a fusion prediction confidence coefficient and a label; and finally, judging the coding potential, if not, outputting a conclusion, if yes, deducing an amino acid sequence, calculating comprehensive sequencing confidence by combining multiple indexes, sequencing, and screening small peptide sets with high and low confidence according to a threshold value. According to the method, an efficient sequence state space modeling structure is introduced, dependence on ribosome sequencing or mass spectrum data is not needed, high-precision, low-complexity and extensible prediction of the potential small peptide coding capacity in the lincRNA sequence is achieved, and the technical defect that accuracy and calculation efficiency are difficult to consider at the same time in an existing method is overcome.

Described herein is a new mitochondrial peptide. This small peptide is capable of modulating cancer, through a variety of mechanisms including autophagy / apoptosis, reduction of tumor cell viability, inducing inflammatory response in senescent cells and conversion of macrophage cell type. Administration of the peptide, its analogs and derivatives thereof, are likely to be effective treatments for cancer therapy, including generation of synthetic analogs that further enhance or abrogate activity relative to the peptide.

The invention discloses a bacillus velezensis strain and an application of the bacillus velezensis strain. The bacillus velezensis strain M4 is obtained by screening soybean paste. The strain is preserved in China Center for Type Culture Collection. The preservation number of the strain is CCTCC (China Center For Type Culture Collection) NO: M 20251427; according to the present invention, the strain has characteristics of broad-spectrum antibacterial ability, high protease and cellulase production ability, and high alpha-amylase and gamma-aminobutyric acid (GABA) production ability, the protease yield is 318.96 + / -0.17 U / mL, and the cellulase yield is 52.63 + / -0.77 U / mL. In the process of fermenting the bean dregs, the bacillus velezensis M4 can inhibit the growth of pathogenic bacteria, obviously change the accumulation mode of amino acids, small peptides and analogues thereof in the bean dregs, improve the protein utilization rate of the bean dregs and obviously improve the flavor of the bean dregs.

The invention discloses an antiviral small peptide and application thereof. The amino acid sequence of the antiviral small peptide is as shown in SEQ ID NO. 1. The invention also discloses a nucleic acid molecule for coding the antiviral small peptide. The antiviral small peptide or the nucleic acid molecule has any one of the following applications: application in inhibition of virus infection, application in preparation of antiviral drugs, and application in preparation of transgenic plants with antiviral ability. The antiviral small peptide provided by the invention can significantly improve the resistance of plants, especially tobaccos, to ChiVMV. The antiviral small peptide is safe, stable, easy to prepare and suitable for large-scale application. The composition can be used for inhibiting virus infection, the resistance of tobacco to ChiVMV is obviously improved, the plant symptoms are obviously relieved after spraying, and the virus accumulation amount of upper leaves is obviously reduced. The spraying effect before and after inoculation is better, the resistance enhancement effect is further improved by spraying in the time period, and the invention provides a new antiviral management strategy.

The application discloses a lincRNA small peptide coding potential identification method and system, and belongs to the technical field of computer technology and bioinformatics. The method first processes lincRNA sequences into a standardized sequence set; then identifies and integrates potential coding fragments to obtain a candidate coding region set; after extracting the multi-dimensional feature codes of each region, the fusion prediction confidence and label are obtained through the Mamba model and the machine learning model; finally, the coding potential is determined, and if there is no coding potential, the conclusion is output, and if there is coding potential, the amino acid sequence is deduced, the comprehensive ranking confidence is calculated and ranked by combining multiple indexes, and the high and low confidence small peptide sets are screened according to the threshold. The application introduces an efficient sequence state space modeling structure, does not need to rely on ribosome sequencing or mass spectrometry data, realizes high-precision, low-complexity and scalable prediction of the potential small peptide coding capacity in the lincRNA sequence, and solves the technical defects that the existing methods are difficult to balance between accuracy and computational efficiency.

This invention discloses the Anti-WOX11 small peptide from Aquilaria sinensis and its application in promoting plant root regeneration. Through genetic transformation, this invention obtained transgenic Aquilaria sinensis and Arabidopsis thaliana containing the Anti-WOX11 fragment. Experiments demonstrated that Anti-WOX11 can promote the regeneration of plant cuttings and the development of lateral roots. This invention fills the research gap on the direct regulation of cutting regeneration by small peptides in Aquilaria sinensis, providing a feasible solution for improving the survival rate of Aquilaria sinensis after root pruning and transplanting. It has great research potential and broad application prospects, and can be applied to promoting agarwood formation and the production and preparation of agarwood-related products.

Disclosed is a pharmaceutical composition to prevent transmission of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the pharmaceutical composition comprising: genetically modified bacteria; sequences of small peptides; and pharmaceutical excipients, wherein the genetically modified oral bacteria are modified to translate, produce, and emit the sequences of small peptides which neutralize SARS-CoV-2 against COVID-19, wherein transgenic technology is used to modify the genetically modified oral bacteria to add genes in genetically modified oral bacteria that are transcribed to produce small peptides from the sequences of small peptides so added, wherein the sequences of small peptides show extreme binding and neutralization to SARS-CoV-2 but not to host proteins or processes, and wherein the pharmaceutical excipients aid the oral and / or nasal administration of the pharmaceutical composition.

The application discloses a kind of complex small molecule coral food and its application in adjusting coral symbiotic microbial community structure.The raw materials of the complex small molecule coral food include: soybean meal small peptide protein, cod small peptide protein, haematococcus pluvialis powder, complex amino acid, squid meal, chitosan oligosaccharide and complex nucleotide.The complex small molecule coral food of the application takes soybean meal small peptide protein and cod small peptide protein as main component, haematococcus pluvialis as secondary component, and is supplemented with complex amino acid and squid meal, and is additionally added with chitosan oligosaccharide and complex nucleotide.The obtained complex small molecule coral food not only has good palatability, but also ensures the feeding amount of coral.It is verified by test that the complex small molecule coral food of the application can adjust the symbiotic microbial community structure of aquarium coral, improve the proportion of coral beneficial symbiotic bacteria, and further improve the environmental tolerance of coral.

Methods and materials for preventing and modulating atherosclerosis. In particular, small peptides that are capable of interacting with CD40, thereby interfering with the ability of CD40 to interact with CD154, which impacts inflammation and atherosclerosis. The use of such peptides in reducing atherosclerosis, and in particular, the autoimmune inflammatory response that may be a driving factor thereof. The use of such short peptides to lower LDL cholesterol. Methods and materials for detecting T-cells that express CD40 (Th40 cells). Methods and materials for preventing, modulating, reducing and / or reversing type 2 diabetes and auto-inflammatory disease. In particular, small peptides that are capable of interacting with CD40, thereby interfering with the ability of CD40 to interact with CD154, which impacts inflammation and type 2 diabetes. The use of such peptides in reducing type 2 diabetes, and in particular, the autoimmune inflammatory response that may be a driving factor thereof. The use of such short peptides to lower IL2, INFγ, and IL17a. The use of such peptides to increase glucose transport protein (GLUT4). Methods and materials for detecting T-cells that express CD40 (Th40 cells).

The application discloses a human head and neck squamous cell carcinoma clinical diagnosis reagent, which is a reagent for detecting the expression amount of a human NCAPH gene. The expression level of the human NCAPH gene is negatively correlated with the clinical prognosis of human head and neck squamous cell carcinoma. Experimental results show that the expression of the human NCAPH gene in a human head and neck squamous cell carcinoma cell line is higher than that in normal nasopharyngeal epithelial cells. Knocking down the expression of the human NCAPH gene can significantly inhibit the proliferation and colony sphere formation ability of the head and neck squamous cell carcinoma cells. The application also provides a drug for inhibiting the expression of the human NCAPH gene or a NPIDP small peptide drug for inhibiting the expression of the human PD-L1 gene. The NPIDP small peptide drug is used on human head and neck squamous cell carcinoma cells, can significantly compete to block the combination of NCAPH and HIP1R, promote the degradation of the PD-L1 protein, and thus enhance the T cell killing effect. The application provides a new diagnosis and treatment drug for the treatment of human head and neck squamous cell carcinoma.

The invention provides a method for preparing an agricultural enzyme from non-commercial corn, and relates to the technical field of biological fermentation. In order to overcome the defects that in the prior art, non-commercial corn is large in output and low in resource utilization rate, and existing agricultural enzymes are high in raw material cost, long in fermentation period, disordered in strain, low in effective component content, single in function and the like, the non-commercial corn which is slightly mildewed, damaged, immature and the like is used as a raw material; the preparation method comprises the following steps: crushing, mixing, carrying out fermentation pretreatment, and then inoculating Gudiniberg Lindnella yeast 413 to carry out liquid aerobic fermentation to obtain fermentation liquor rich in viable bacteria, amino acids, small peptides, vitamins and enzyme activity, so as to directly prepare the agricultural enzyme. According to the method, raw materials are low in price and easy to obtain, the fermentation period is short, the content of viable bacteria is high, the product has the fertilizer efficiency and the biocontrol function, the crop yield and quality can be remarkably improved, and the problems of non-commodity corn shelving and high agricultural microbial fertilizer cost are solved. According to the method, non-commercial corn can be well utilized in a value-added mode, and the ferment is produced to be used for soil and fertilizer to improve the crop yield.

The application belongs to the field of molecular biological technology, and specifically discloses application of a small peptide miPEP171b in improving cold resistance of grape. The miPEP171b is derived from grape, and the amino acid sequence is MKGPGGKAGHLIERPSV*. The application proves that the miPEP171b has translation activity, is induced to express under low temperature, can regulate transcription of pri-miR171b in a positive direction, promote accumulation of mature vvi-miR171, and inhibit expression of a target gene VvSCL15 / VvSCL27, effectively improves cold resistance of grape, and is helpful to deal with disastrous weather such as "early spring cold". The application provides two methods of improving cold resistance of grape, i.e. exogenous application of the miPEP171b small peptide and overexpression of a coding gene of the miPEP171b, and provides a brand-new molecular target and application strategy for crop stress resistance breeding and stress resistance cultivation.

This application relates to site-specific conjugation of antibody lysine residues using solid phase immobilized microbial transglutaminase MTG and MTG in solution. Site-specific modification of proteins using microbial transglutaminase (MTG) is a powerful and versatile strategy for controlled modification of proteins under physiological conditions. We present evidence that solid phase microbead immobilization can be used to site-specifically and efficiently link different functional molecules important for further downstream applications to therapeutically relevant proteins, including scFV, Fab fragments and antibodies. We demonstrate that MTG remains firmly immobilized and there is no detectable column bleed and the enzyme activity is maintained during continuous operation, which allows for convenient recycling of the enzyme, thus outperforming solution phase MTG conjugation. Furthermore, it is shown that immobilized MTG exhibits an enhanced selectivity for certain residues in the presence of several reactive residues, all of which are targeted if conjugation is performed in solution. Site-specific lysine conjugation of antibodies using immobilized and solution MTG with a highly efficient glutamine-containing peptide is also reported. In addition, the generation of bis-site-specific conjugated IgGl with site-specific conjugation of both glutamine and lysine residues of an IgGl antibody using immobilized and solution MTG is reported. Site-specific glutamine conjugation with small peptides containing lysine residues and functional moieties is also described.

The application relates to the technical field of animal feed processing, and discloses a pork pig fattening feed based on composite microbial fermentation and application thereof, wherein the feed comprises basic feed and nutrient-loaded biochar; the biochar is loaded with amino acids and trace elements and has pH response characteristics: stable adsorption in a neutral environment with pH 6.0-7.0, and release in an acidic environment with pH 4.0-5.0. The application also provides a three-stage fermentation process, through second-stage lactic acid bacteria acidification, triggering the biochar to release nutrients in the third stage (spore bacillus proliferation stage). The application realizes timed supply of nutrients, significantly improves the enzyme activity, small peptide content and spore bacillus viable count of the feed, and shows lower feed-meat ratio and diarrhea rate in animal feeding.

Disclosed herein are small anti-inflammatory peptides for blocking NF-κB. The peptides and compositions including the peptides can be administered to inhibit cytokine production and / or to inhibit lung inflammation in individuals in need thereof. Particular embodiments include administration to individuals suffering from coronavirus disease 2019 (COVID-19).

This application discloses an immunoassay kit, its preparation method, and its application. The immunoassay kit includes a small peptide-coated solid-phase carrier, a bispecific antibody, and a labeled detection antibody. The bispecific antibody includes an anti-small peptide antibody and a linked capture antibody. The anti-small peptide antibody specifically binds to the small peptide, and both the capture antibody and the labeled detection antibody specifically bind to the target analyte and have different antigen recognition epitopes. The highly specific binding of the small peptide to the anti-small peptide antibody achieves efficient immobilization of the bispecific antibody, significantly improving antibody immobilization density and activity retention compared to traditional detection methods. The targeted binding effect of the bispecific antibody amplifies the detection signal while avoiding competitive interference from endogenous biotin, achieving highly sensitive and interference-resistant immunoassay.