275 results about "Small peptide" patented technology

The invention provides copper-loaded duck-origin lactobacillus plantarum with high antibacterial peptide yield and application of the copper-loaded duck-origin lactobacillus plantarum. The preservation number of the provided copper-loaded duck-origin lactobacillus plantarum is CGMCC (China General Microbiological Culture Collection Center) NO.32835. The domesticated and bred duck-origin lactobacillus plantarum with high antibacterial peptide yield can tolerate high-concentration copper ions, the domesticated and bred lactobacillus plantarum can stably grow, and the contents of antibacterial peptide, extracellular protein, lipase, amylase, lactic acid, acetic acid and the like in the domesticated and bred lactobacillus plantarum strain are increased; and the amino acid composition is changed. The strain can convert inorganic copper into organic copper, the utilization rate of copper is increased, and growth and development of organisms are promoted. Moreover, small peptides and extracellular proteins in metabiotics generated after fermentation have the effect of effectively inhibiting harmful bacteria such as riemerella anatipestifer, escherichia coli, salmonella and staphylococcus of poultry, and are beneficial to eliminating intestinal inflammatory factors, maintaining intestinal health and improving the feed utilization rate.

The present invention relates to ARP2 of plant stigma cells; the invention relates to application of Ca < 2 + > transport capacity damage of homologous genes 1 and 1 in breaking through reproduction isolation of plants. According to the method for breaking through the plant reproductive isolation, a foundation is laid for further research on the relationship of small peptides for regulating and controlling reproductive isolation calcium signals through co-receptors, and a simple and effective target is provided for gene modification for breaking through the reproductive isolation.

The invention belongs to the field of corn disease prevention, relates to a novel small peptide, provides a small peptide NCRP246 and application thereof in improvement of corn southern leaf blight resistance, and provides feasibility for plant disease resistance by using non-classical peptides. A corn southern leaf blight resistance identification experiment proves that NCRP246 can significantly improve the resistance of corn to southern leaf blight, and further researches that the NCRP246 reduces oxidative damage of pathogenic bacteria infection of the southern leaf blight to the corn by reducing H2O2 accumulation so as to mediate the resistance of the corn to the southern leaf blight; the analysis from the molecular perspective proves that the NCRP246 can induce the expression of defense related genes.

The invention relates to the technical field of molecular biology, and discloses application of TaPSY5 small peptide and a coding gene thereof in promoting plant root growth. A tyrosine sulfated polypeptide is named as TaPSY5 small peptide and is as follows: (a) mature small peptide composed of 18 amino acids shown in a sequence 1 in a sequence table and having tyrosine sulfated modification; the TaPSY5 small peptide precursor protein is the following (b) or (c): (b) a protein composed of amino acids as shown in a sequence table 2; and (c) a protein derived from the amino acid of the sequence 2 in the sequence table through deletion / or substitution / or insertion of one or more amino acids. The TaPSY5 small peptide is externally applied, so that the growth rate of a plant root system can be increased. The gene (TaPSY5 gene) for coding the TaPSY5 precursor small peptide also belongs to the protection range of the invention. The invention also protects that the TaPSY5 gene is introduced into a target plant, and lateral roots and main roots of the obtained transgenic plant are significantly more than those of the target plant.

The invention provides application of small peptides in preventing and treating plant diseases and promoting plant growth. The structural formula of the small peptide is shown in the specification. Experimental results show that the small peptide shown in the formula A has the effects of improving the disease resistance of plants and regulating the growth of the plants. The small peptide not only can be used as a plant immune resistance inducer at low concentration to improve the immunocompetence of plants for resisting pathogen invasion, but also can promote plant growth, and has a good application prospect. R1-R2-beta-Ala-Trp-Gly-NH2 Formula A

The invention relates to the field of agricultural products, and particularly discloses a polypeptide for improving plant disease resistance, and the polypeptide is LAND2 small peptide and homologous peptide thereof. The LAND2 small peptide disclosed by the invention is screened and found from an arabidopsis thaliana proteome, and homologous peptides of the LAND2 small peptide are respectively derived from chilies and cucumbers. Tests prove that when the LAND2 small peptide is externally applied to arabidopsis thaliana, the local and systematic resistance of plants to botrytis cinerea can be enhanced, and the LAND2 small peptide has broad-spectrum immunocompetence in chilies and cucumbers. The polypeptide is practically applied to agricultural production, can enhance the disease resistance of plants and improve the yield and quality of the plants, and has practical popularization and application values.

The invention discloses a small peptide for improving abiotic stress of tartary buckwheat and application of the small peptide, and relates to the technical field of biotechnology and botany, the small peptide is FtRALF6, and the amino acid sequence of the FtRALF6 is as shown in SEQ ID No.1. By exogenous application of the FtRALF6 small peptide, salt stress resistance, heavy metal stress resistance, cold stress resistance and heat stress resistance of the tartary buckwheat can be remarkably improved, normal growth and physiological functions of the tartary buckwheat under adversity are effectively maintained, and yield and quality are improved. The small peptide is small in molecular weight, easy to absorb, environment-friendly and large in application potential, the tartary buckwheat planting range can be expanded, the planting economic benefits are improved, and a new thought is provided for crop stress resistance research and technical development.

The invention discloses an application of a small peptide PEP914 or PEP890 in preparation of a biopesticide, and belongs to the technical field of biology. The biopesticide comprises a small peptide PEP914 or PEP890 and is a biopesticide for resisting bacterial leaf spot, rust disease or phytophthora root rot, the amino acid sequence of the small peptide PEP914 is shown as SEQ ID No.1, and the amino acid sequence of the small peptide PEP890 is shown as SEQ ID No.2. The small peptide PEP914 or PEP890 is applied to preparation of the biopesticide, a novel biopesticide thought is provided, and the problems that in the prior art, chemical pesticides are prone to remaining, environment problems are prone to being caused, and pathogens generate drug resistance are solved.

The invention provides a preparation process of micromolecular cubilose peptide and a compound tablet thereof, and belongs to the technical field of protein and peptide. The preparation process of the micromolecular cubilose peptide comprises the following steps: freezing and crushing cubilose; and carrying out compound enzyme enzymolysis. The preparation method comprises the following steps: repeatedly freezing and crushing cubilose to prepare cubilose powder, adding the cubilose powder into deionized water, heating and stewing, and adding a compound enzyme formed by mixing neutral protease and flavourzyme for ultrasonic-assisted enzymolysis to obtain the micromolecular cubilose peptide, when the neutral protease and the flavourzyme are mixed according to the mass ratio of 1: (2.8-3.4) to conduct enzymolysis on the cubilose, hydrolysis can be conducted from multiple positions, smaller peptide fragments are formed, the hydrolysis degree is effectively improved, sialic acid originally combined with protein is also released along with hydrolysis of the protein, and therefore the content of sialic acid in the cubilose is increased. Therefore, the content of sialic acid in the prepared micromolecular cubilose peptide can be effectively improved.

The invention belongs to the technical field of biotechnology and botany, and particularly relates to a small peptide for improving abiotic stress tolerance of crops and application of the small peptide, and the small peptide is GhIDL02 and / or GhPIPL7. The amino acid sequence of the GhIDL02 is as shown in SEQ ID NO. 1; the amino acid sequence of the GhPIPL7 is as shown in SEQ ID NO. 2. According to the method, the small peptide GhIDL02 or GhPIPL7 is exogenously applied, so that the hormone-like effect is quickly exerted, the salt tolerance of the cotton is remarkably improved, and the adverse effect of a chemical regulating agent on the environment is avoided. Through a research technology combining bioinformatics and molecular biology, the positive regulation effect of the GhIDL02 and GhPIPL7 small peptides under salt stress is confirmed for the first time, and by activating an MAPK signal channel, the steady state of cotton sodium and potassium ions is maintained, the salt tolerance of cotton is improved, and a theoretical basis and technical support are provided for green development of an agricultural biotechnology.

The invention relates to the technical field of antibacterial peptides, and discloses a preparation method and application of a multi-segment fused antibacterial peptide fusion protein. The antibacterial peptide fusion protein is obtained by introducing OST signal peptide and collagen and optimizing the connection sequence of the OST signal peptide, the collagen, bovine lactoferrin peptide LFcinB, bovine lactoferrin N-leaf small peptide LFcin and bovine lactoferrin N-leaf derived peptide Lf. The antibacterial peptide fusion protein can simultaneously contain three segments of bovine lactoferrin active peptides, namely LFcinB, LFcin and Lf, and the yield of the fusion peptide is maximized. Experiments prove that the fermentation yield of the fusion protein comprising the OST signal peptide, the collagen, the bovine lactoferrin peptide LFcinB, the bovine lactoferrin N-leaf small peptide LFcin and the bovine lactoferrin N-leaf derived peptide Lf which are sequentially connected from the N terminal to the C terminal can reach 600mg / L.

The invention relates to the technical field of biological agents, in particular to abalone-derived polypeptide with antioxidant activity and a preparation method thereof. According to the invention, Haliotis discus hannai Ino is taken as a research object, a small peptide library is established by using a virtual enzymolysis technology from an important anti-oxidation pathway of cells, namely a Keap1-Nrf2 pathway, an abalone-derived anti-oxidation peptide DEDEDEDK with relatively strong binding capacity with Keap1 is screened out by using a molecular docking technology, and then, the anti-oxidation activity of the abalone-derived anti-oxidation peptide DEDEDEDK is verified by using a cell oxidative damage model, so that the abalone-derived anti-oxidation peptide DEDEDEDK can be used for preparing the anti-oxidation peptide DEDEDEDK with relatively strong binding capacity with Keap1. And a theoretical basis is provided for research and application of the antioxidant peptide.

The invention discloses a method for realizing high-efficiency selenium labeling of protein, which comprises the following steps of: in the process of expressing plasmids containing target protein through a prokaryote expression system, realizing high-efficiency replacement of cysteine sulfur atoms in the target protein by selenium atoms by regulating and controlling the ratio of selenium to sulfur elements in a culture medium; on the premise of keeping a good growth state of cells, the selenium labeling efficiency can reach 86%, and the protein expression quantity is stabilized at about 1mg / g (protein / thallus wet weight); structural analysis shows that the secondary structure of the obtained selenium-labeled protein is basically consistent with that of natural protein and is not obviously changed. The method is easy and convenient to operate, high in labeling efficiency, high in protein yield, good in repeatability and suitable for structural biology research such as nuclear magnetic resonance, an effective means is provided for research of the functional mechanism of sulfur atoms in biomacromolecules (including small peptides, polypeptides, peptide fragments, proteins and protein or peptide complexes) with selenium as a probe, and the method has a wide application prospect. Good scientific research application prospects and industrial popularization values are realized.

The invention relates to a construction method and application of a plant intra-genus generic transcriptome, and the method is realized by the following steps: obtaining second and third generation transcriptome data of different species in a genus and performing quality control, comparing a reference genome by using software such as HISAT2 and the like, and screening effective transcripts through gffcomplex after StringTie splicing; transcripts and genes are classified based on the ORF length (mRNA is greater than or equal to 100 aa, small peptide transcripts are from 10 aa to 99 aa, and ncRNA is smaller than 10 aa); the method comprises the following steps: analyzing sequence conservative properties among species through coding genes and non-coding genes, and dividing the genes into core, optional and specific types; and finally integrating and constructing a genus-level generic transcriptome containing three types of transcriptomes. According to the invention, the system construction of the plant intra-genus cross-species pan transcriptome is realized for the first time, the conservative property and difference of protein coding genes, small peptides and non-coding RNA can be analyzed, and a new tool is provided for the research of plant functional genomes.

The present invention discloses a preparation method of antioxidant sturgeon protein peptides, comprising the following steps: S1, extraction of crude sturgeon skin protein solution; S2, extraction of crude sturgeon swim bladder protein; S3, extraction of crude sturgeon meat protein solution; S4, mixed enzymatic hydrolysis; S5, subjecting the obtained enzymatic hydrolysis product to enzyme inactivation treatment first to obtain an enzymatic hydrolysis solution, and then sequentially ultrafiltering and vacuum drying the enzymatic hydrolysis solution to obtain antioxidant sturgeon protein peptides. The present invention mixes the proteins extracted from different parts of sturgeon in a specific ratio, and then performs enzymatic hydrolysis. The neutral protease and papain added can act synergistically to more comprehensively hydrolyze proteins, generating small peptides with specific functions. At the same time, glutathione added during enzymatic hydrolysis can protect the sulfhydryl groups of the enzyme and maintain the enzyme activity, so that the finally obtained sturgeon protein peptides have good antioxidant properties.

The invention discloses an antibacterial small peptide CsCAPE9 and application of the antibacterial small peptide CsCAPE9 in prevention and control of citrus huanglongbing. The amino acid sequence of the antibacterial small peptide CsCAPE9 is as shown in SEQ ID NO.1; the antibacterial small peptide CsCAPE9 provided by the invention is a small peptide synthesized in a plant body, and the antibacterial small peptide CsCAPE9 can activate biosynthesis of salicylic acid and induce systematic defense reaction of citrus. The antibacterial small peptide CsCAPE9 has a good inhibition effect on pathogenic bacteria of citrus huanglongbing in greenhouses and fields, and has a wide application prospect in comprehensive prevention and control of the huanglongbing.

The invention relates to the technical field of fertilizers, in particular to a preparation method of a chelated medium element preparation for a synergistic liquid fertilizer. The technical problems that a chelating agent is high in cost, poor in stability and low in bioavailability are solved. The efficient chelating agent is prepared by efficiently extracting or converting a polysaccharide-polyphenol compound and a small peptide mixture with unique chelating performance from specific agricultural or industrial wastes and mixing the polysaccharide-polyphenol compound and the small peptide mixture. Then, a chelating-wrapping dual-stabilization technology is adopted, specifically, a high-efficiency chelating agent and medium element ions are subjected to a chelating effect, and then modified chitosan is added to wrap the chelate, so that nutrient loss is reduced, and nutrient elements are released more evenly. The chelated medium element preparation remarkably improves the stability and effectiveness of nutrient elements through the synergistic effect of double stabilization mechanisms.

The invention relates to the technical field of bioengineering, and discloses an elastin peptide fusion protein as well as a preparation method and application thereof. Based on the elastin with an amino acid sequence such as SEQ ID NO.8, high-yield expression of the elastin is realized through pichia pastoris GS115. According to the elastin, bovine lactoferrin peptide with a natural antibacterial function is fused, bovine lactoferrin N-leaf small peptide, bovine lactoferrin peptide and elastin peptide with an amino acid sequence as shown in SEQ ID NO.8 are fused, on the premise that the high expression quantity of the fusion protein is guaranteed, the fusion protein is endowed with more functions, and the fusion protein has the advantages of being capable of resisting bacteria and resisting bacteria. Therefore, the fusion protein can be applied to cosmetics, such as artificial blood vessels and other medical equipment products, and the elastin has good biocompatibility and a natural preservative function.

The invention discloses a nanoparticle targeting bone tissue macrophages as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. According to the invention, a dual targeting mechanism is adopted, and the surface of the nanoparticle is modified with a bone-like small peptide sequence and a mannose modified ligand, so that the nanoparticle can specifically recognize macrophages in a bone tissue environment. According to the bone tissue macrophage targeting nanoparticle system prepared by the invention, the targeting efficiency, the anti-inflammatory effect and the tissue repair effect can be remarkably improved, systematic side effects are reduced, a new treatment means is provided for bone injury repair, and the bone tissue macrophage targeting nanoparticle system has a good clinical application prospect.

The invention discloses a polypeptide for inhibiting and reversing pathological aggregation of human crystalline lens protein gamma D. According to the invention, alpha B-crystalline lens protein is cut into small peptides, and the capability of inhibiting and reversing pathological aggregation of crystalline lens protein is detected, so that the optimal polypeptide targeting the gamma D-crystalline lens P23T mutant protein is screened out. The polypeptide screened by the invention can significantly inhibit the aggregation of the gammaD-lens P23T mutant protein, and can reverse the aggregation of the gammaD-lens P23T mutant protein, which indicates that the polypeptide provided by the invention has a potential effect of treating cataract, and has a wide application prospect clinically.

The invention discloses saccharomyces boulardii, a culture and a preparation method and application of the saccharomyces boulardii. The preservation number of the strain is CCTCC (China Center for Type Culture Collection) NO: M 20252633. The preparation method comprises the following steps: preparing saccharified liquid by taking white spirit vinasse or brewer's grains as a main raw material and adopting a high-temperature alkali treatment and composite enzymolysis process, inoculating strains for liquid-state high-density fermentation, carrying out solid-liquid separation on fermentation liquor, taking a liquid phase, and carrying out spray drying. According to the method, high-value utilization of the waste vinasse is realized, and the production cost is remarkably reduced. The obtained culture is rich in viable bacteria and active small peptides, and has the characteristics of strong tolerance, wide antibacterial spectrum and the like. As a feed additive, the feed additive can effectively reduce the diarrhea rate of piglets, inhibit clostridium perfringens and prawn vibrios of broilers, and improve the oxidation resistance and production performance of animals.

The invention provides a peptide-enriched organic fertilizer as well as a preparation method and application thereof, and belongs to the technical field of organic fertilizer preparation. The preparation method of the peptide-enriched organic fertilizer provided by the invention comprises the following steps: mixing corn soaking water with aspergillus oryzae seed koji, and fermenting for the first time to obtain the peptide-enriched organic fertilizer; the aspergillus oryzae mold starter is obtained by inoculating aspergillus oryzae YY-21 into a culture medium and fermenting 2. According to the preparation method provided by the invention, the peptide-enriched organic fertilizer with high amino acid nitrogen content, high small peptide content, high soluble protein content and high thallus biomass can be prepared, and when the peptide-enriched organic fertilizer is used for planting lettuce, the plant height, the fresh weight of a single plant, the root length, the leaf number and the leaf area of the lettuce can be remarkably increased; the chlorophyll content, the vitamin C content and the total sugar content in the lettuce can be remarkably improved, and the nitrate content of lettuce leaves can be reduced.

The invention relates to a method for screening tumor autoantibodies by using a small peptide display library. The method comprises the following steps: S1, obtaining a tumor mutation small peptide display library; s2, screening to obtain small peptides capable of interacting with serum; s3, analyzing protein and mutation information in the tumor mutation material to obtain an analysis result; and S4, calculating to obtain protein and mutation information with significant difference. Therefore, on the basis of obtaining a tumor mutation material (related), serum immunoprecipitation and high-throughput sequencing are combined, and a strategy for globally and unbiasedly researching the mutation-related tumor autoantibody is provided.

The invention discloses a method for improving digestion characteristics of frozen meat under assistance of ultrasonic waves, which comprises the following steps: cutting fresh meat blocks, sealing, and refrigerating at 0-4 DEG C for 4-8 hours; and freezing the refrigerated meat blocks, applying ultrasonic waves in the freezing process, setting the ultrasonic waves to be in a circulating intermittent mode when the central temperature of the sample reaches-0.5 DEG C to 0.5 DEG C, and completely immersing the meat blocks into refrigerant industrial alcohol until the geometric central temperature of muscles is reduced to-18 DEG C, thereby completing freezing. According to the present invention, with the treatment method of auxiliary ultrasonic application on the sample during the freezing process, the nutrition damage and the digestion characteristic reduction caused by the freezing of the sample are reduced, such that the sample has the high digestion rate, the protein is completely decomposed into the small peptide or the amino acid, the protein particles are small and do not aggregate, and the sample has the high nutrition value; the ultrasonic-assisted freezing proves that the digestion characteristic of the frozen meat is improved to a certain extent.

The invention discloses application of an arabidopsis proPep1 mutant gene and an encoded protein thereof in resisting potyvirus viruses, and belongs to the technical field of plant genetic engineering. In order to solve the technical problems that in the prior art, potyvirus resistance resources are few, prevention and control are difficult, and environmental pollution is easily caused by chemical prevention and control of insect propagation mediators, the invention provides an arabidopsis proPep1 mutant gene and an application of an encoding protein thereof in resisting potyvirus viruses, the nucleotide sequence of the arabidopsis proPep1 mutant gene is as shown in SEQ ID NO.2, and the encoding protein of the arabidopsis proPep1 mutant gene has a nucleotide sequence as shown in SEQ ID NO.1. The arabidopsis proPep1 mutant gene has a nucleotide sequence as shown in SEQ ID NO.2. And a coding protein sequence is shown as SEQ ID NO.4, and a plant elicitor small peptide Pep1 expression vector mature by NIa-Pro protease hydrolysis and a transgenic disease-resistant plant are constructed. The disease-resistant system provided by the invention achieves the purpose of enhancing the potyvirus virus resistance of plants, and can be applied to prevention and treatment of diseases caused by potyvirus viruses.

The invention discloses a plant endogenous small molecule polypeptide and application thereof in inhibiting RNA virus infected plants, the sequence of the small molecule polypeptide is Pep1-3: IIGVSNFDPVKRS, and the small molecule polypeptide is derived from nicotiana benthamiana; the invention relates to application of plant endogenous small molecule polypeptide in inhibiting RNA virus infection of plants. The plant is tobacco; the RNA viruses comprise tobacco mosaic viruses, tomato mosaic viruses and turnip mosaic viruses, and the small peptide Pep1-3 serves as a novel plant immunity inducer and shows the potential of the small peptide Pep1-3 in the aspect of preventing and controlling the RNA viruses. In agricultural production, prevention and treatment of virus diseases by adopting oligopeptide substances are a green and sustainable direction, and compared with traditional pesticides and plant immunity inducers, the composition has the advantages of broad spectrum, small toxicity, no drug resistance risk and long lasting period, and can generate a synergistic or synergistic effect when being mixed with other medicaments.

The disclosure provides an immunomodulatory octapeptide, and this small peptide molecule has a unique structure, higher broad-spectrum antibacterial activity, stronger pathogenic bacteria inhibition and high purity, can maintain stability of its structure and antibacterial activity in artificial gastric juice, with no obvious hemolytic activity, and has high application value in animal husbandry. The disclosure provides a preparation method and a purity test method of this immunomodulatory octapeptide.

The invention discloses an application of a small peptide GLP-1 (28-36) amide in preparation of a medicine for preventing and treating rice blast and a method of the small peptide GLP-1 (28-36) amide. The invention discloses an application of the small peptide GLP-1 (28-36) amide in preparation of a medicine for preventing and treating magnaporthe oryzae, and an application of the small peptide GLP-1 (28-36) amide in inhibition of magnaporthe oryzae and prevention and treatment of magnaporthe oryzae. The small peptide GLP-1 (28-36) amide can effectively inhibit pathogenicity of pyricularia oryzae spores in the concentration range of 12.5-100 [mu] M, especially the small peptide GLP-1 (28-36) amide with the concentration of 100 [mu] M can completely prevent and treat pyricularia oryzae, and the small peptide GLP-1 (28-36) amide can be used for developing novel green pesticides.

The invention relates to the technical field of marine product processing, in particular to a preparation method of high-activity fish protein powder, which comprises the following steps: cleaning and crushing deep-sea fish, transferring into an enzymolysis tank, adding brown sugar and / or molasses, yeast extract and clean water, and adding metalloproteinase and biological coenzyme for preliminary enzymolysis; bacterial enzymes such as bacillus velezensis, halophilic bacillus and Fengda salt-tolerant microorganisms are introduced for degradation; and mixing the bacterial enzyme degradation liquid with a chitosan solution, and performing spray drying to obtain the high-activity fish protein powder. According to the invention, macromolecular proteins are decomposed into small peptides and free amino acids by utilizing metalloproteinase and biological coenzyme through the synergistic effect of bacteria and enzymes, and microbial fermentation is introduced, so that the small peptides and other organic substances are further decomposed, efficient and comprehensive multi-stage degradation of fish proteins is realized, and release of nutritional active ingredients is maximized; finally, the fish protein powder is protected through a nano drying medium, and the stability and safety of the fish protein powder are improved.

The present invention provides a saccharomyces cerevisiae culture and its application. The saccharomyces cerevisiae culture is obtained by utilizing food processing byproducts such as soybean molasses, pomace, and coffee grounds under specific conditions using the saccharomyces cerevisiae strain HKB-145007 (deposit number CGMCC No. 24839). The saccharomyces cerevisiae culture is rich in active substances such as protein, mannooligosaccharides, small peptides, organic acids, and polyphenols. When added to a complete mixed diet for dairy cows at a ratio of 3% to 5%, or used in combination with a drug for treating mastitis in dairy cows, it has a positive effect on preventing and treating mastitis in dairy cows.