Complex bacterium for bean pulp fermentation and application of complex bacterium
A technology of complex flora and soybean meal, applied in the field of microorganisms, can solve problems such as unreasonable selection of fermentation strains, unreasonable nutritional value, incomplete fermentation of soybean meal, etc., to improve digestion utilization rate, good food-attracting effect, and inhibit the growth of pathogenic bacteria Effect
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Embodiment 1
[0012] Embodiment 1: Preparation of liquid composite flora
[0013] The bacterial strains of the composite flora are respectively subjected to plate activation, culture in small shake flasks, culture in large shake flasks, and step-by-step amplified culture in fermentors using their corresponding culture medium to obtain bacterial fluids of various strains. The number of viable bacteria in Bacillus subtilis, Bacillus coagulans, Lactobacillus plantarum and Saccharomyces cerevisiae shall not be less than 1×10 8 cfu / mL, the number of viable bacteria in Aspergillus niger, Aspergillus oryzae and other bacterial liquids is not less than 1×10 7 cfu / mL. Mix the bacterial liquids of various strains in proportion to obtain the liquid composite bacterial group. The number of live bacteria in the liquid complex flora is not less than 5×10 8 cfu / mL.
[0014] The liquid composite flora can also be obtained by mixing commercially available liquid strains of various strains in proportion....
Embodiment 2
[0015] Embodiment 2: preparation of solid composite flora
[0016] The bacterial strains of the composite flora are respectively subjected to plate activation, culture in small shake flasks, culture in large shake flasks, and step-by-step amplified culture in fermentors using their corresponding culture medium to obtain bacterial fluids of various strains. The number of live bacteria in Bacillus subtilis, Bacillus coagulans, Lactobacillus plantarum and Saccharomyces cerevisiae shall not be less than 5×10 8 cfu / mL, the number of viable bacteria in Aspergillus niger, Aspergillus oryzae and other bacterial liquids is not less than 1×10 8 cfu / mL. The solid bacterial agents are obtained by carrier adsorption, freeze-drying, spray-drying or solid matrix culture, etc., and the solid bacterial agents of each bacterial species are mixed in proportion to obtain a solid composite bacterial group. The number of live bacteria in the solid composite flora is not less than 2×10 8 cfu / g. ...
Embodiment 3
[0018] Embodiment 3: detection method
[0019] Moisture content is tested according to GB / T 6435-2006, crude protein content is tested according to GB / T 6432-1994, small peptide content is determined by TCA method, and trypsin inhibitor is detected by BAPA method with reference to literature (Li Sufen, Huo Guicheng, Yang Lijie , et al. Study on the content of trypsin inhibitor in the main soybean varieties in Heilongjiang Province[J]. Assay [J]. Biotechnology, 1996, 6(5): 44-46.). After testing, the moisture content of soybean meal raw materials is 12.1%, the crude protein content is 45.5%, the small peptide content is 1.1%, the trypsin inhibitor content is 1872TIU / mg, the soybean hemagglutinin potency is 10300, and the beany smell.
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