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Complex bacterium for bean pulp fermentation and application of complex bacterium

A technology of complex flora and soybean meal, applied in the field of microorganisms, can solve problems such as unreasonable selection of fermentation strains, unreasonable nutritional value, incomplete fermentation of soybean meal, etc., to improve digestion utilization rate, good food-attracting effect, and inhibit the growth of pathogenic bacteria Effect

Inactive Publication Date: 2015-07-08
HENAN UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are many studies on soybean meal fermentation and production of fermented soybean meal enterprises, and the strains and processes used are also different. Generally speaking, the main problems are as follows: unreasonable selection of fermentation strains, incomplete fermentation of soybean meal, long fermentation period, Unstable product quality, nutritional value needs to be improved, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Embodiment 1: Preparation of liquid composite flora

[0013] The bacterial strains of the composite flora are respectively subjected to plate activation, culture in small shake flasks, culture in large shake flasks, and step-by-step amplified culture in fermentors using their corresponding culture medium to obtain bacterial fluids of various strains. The number of viable bacteria in Bacillus subtilis, Bacillus coagulans, Lactobacillus plantarum and Saccharomyces cerevisiae shall not be less than 1×10 8 cfu / mL, the number of viable bacteria in Aspergillus niger, Aspergillus oryzae and other bacterial liquids is not less than 1×10 7 cfu / mL. Mix the bacterial liquids of various strains in proportion to obtain the liquid composite bacterial group. The number of live bacteria in the liquid complex flora is not less than 5×10 8 cfu / mL.

[0014] The liquid composite flora can also be obtained by mixing commercially available liquid strains of various strains in proportion....

Embodiment 2

[0015] Embodiment 2: preparation of solid composite flora

[0016] The bacterial strains of the composite flora are respectively subjected to plate activation, culture in small shake flasks, culture in large shake flasks, and step-by-step amplified culture in fermentors using their corresponding culture medium to obtain bacterial fluids of various strains. The number of live bacteria in Bacillus subtilis, Bacillus coagulans, Lactobacillus plantarum and Saccharomyces cerevisiae shall not be less than 5×10 8 cfu / mL, the number of viable bacteria in Aspergillus niger, Aspergillus oryzae and other bacterial liquids is not less than 1×10 8 cfu / mL. The solid bacterial agents are obtained by carrier adsorption, freeze-drying, spray-drying or solid matrix culture, etc., and the solid bacterial agents of each bacterial species are mixed in proportion to obtain a solid composite bacterial group. The number of live bacteria in the solid composite flora is not less than 2×10 8 cfu / g. ...

Embodiment 3

[0018] Embodiment 3: detection method

[0019] Moisture content is tested according to GB / T 6435-2006, crude protein content is tested according to GB / T 6432-1994, small peptide content is determined by TCA method, and trypsin inhibitor is detected by BAPA method with reference to literature (Li Sufen, Huo Guicheng, Yang Lijie , et al. Study on the content of trypsin inhibitor in the main soybean varieties in Heilongjiang Province[J]. Assay [J]. Biotechnology, 1996, 6(5): 44-46.). After testing, the moisture content of soybean meal raw materials is 12.1%, the crude protein content is 45.5%, the small peptide content is 1.1%, the trypsin inhibitor content is 1872TIU / mg, the soybean hemagglutinin potency is 10300, and the beany smell.

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Abstract

The invention provides a complex bacterium for bean pulp fermentation. The complex bacterium is composed of bacillus subtilis, bacillus coagulans, aspergillus niger, aspergillus oryzae, lactobacillus plantarum and saccharomyces cerevisiae. The complex bacterium provided by the invention is obtained through repeated scientific experiment and reasonable combination based on physiological metabolism feature of the microorganism, the complex bacterium is vigorous in growth metabolism, is capable of secreting a plurality of enzymes, acids and other metabolites, and is capable of efficiently decomposing macromolecule protein, anti-nutritional factors and sensitization factors in the bean pulp, obviously increasing content of soluble protein, small peptide, organic acid, protease and vitamin and the number of probiotics and antibacterial peptide, improving the digestion utilization rate, inhibiting the growth of pathogenic bacteria and reducing the use of antibiotic; and meanwhile, the complex bacterium has acid fragrance of fermentation and is good in food attractant effect. The bacteria strains forming the complex bacterium are included in a feed additive variety catalogue (2013) issued by the department of agriculture, the complex bacterium is safe and reliable, and can be directly used for the industrial production for the bean pulp fermentation.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a soybean meal fermentation technology, in particular to a complex flora used for soybean meal fermentation. Background technique [0002] With high crude protein content and balanced amino acid composition, soybean meal has always been one of the most important plant-source proteins for livestock and poultry. However, there are also anti-nutritional factors such as trypsin inhibitors, urease, soybean hemagglutinin and allergenic factors such as soybean antigen protein in soybean meal, which reduces the digestion and utilization rate of soybean meal and even adversely affects the growth and health of animals. The study found that the use of microbial fermentation to treat soybean meal can effectively reduce the content of anti-nutritional factors and allergenic factors in soybean meal, and at the same time improve the nutritional value and digestibility of soyb...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/18C12N1/14A23K1/14A23K1/00C12R1/125C12R1/685C12R1/69C12R1/25C12R1/865
CPCC12N1/20C12N1/14C12N1/18
Inventor 陈亮惠明王金水黄亮刘娜
Owner HENAN UNIVERSITY OF TECHNOLOGY
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