43results about How to "Inoculate evenly" patented technology

The invention relates to a multifunctional biological solid fermentation tank. The main body structure of the fermentation tank is a horizontal propeller, and spiral auger propeller blades are installed below the tank bottom. An auger is directly communicated with steam for sterilizing form the bottom of the tank and communicated with air for cooling, a spray tube on the upper part of the tank body sprays bacterial liquid for inoculation and can also spray water mist for humidity regulation, and thus, uniform inoculation and humidity control can be realized. The unique tank body horizontal type propeller and the auger coordination system below the tank body are utilized, and the circular flowing and the thorough mixing of a solid material in the tank body during fermentation are realized by controlling the rotating speed and the rotating direction; stirring nonuniformity caused by using a motor to rotate forwards or backwards repeatedly is avoided, automatic material discharge can be realized after fermentation, and material discharge is clean and complete. The operations of sterilization, cooling, inoculation, fermentation, temperature control, humidity control, ventilation, vacuum drying and the like required by solid fermentation can all be completed in the biological solid fermentation tank. The biological solid fermentation tank has the advantages of hardly no bacterial contamination, small occupied space, high automation degree, labor saving, investment saving and the like.

The invention discloses a dry fermentation device and a dry fermentation method to prepare biogas through high-concentration solid raw materials. The raw materials are reasonably distributed, inoculation is uniform, acidification is not prone to occurrence, organic matter degradation is sufficient, the materials are discharged easily, a function of solid-liquid separation is provided, secondary pollution is avoided, biogas residue can be converted into fertilizer, and therefore the dry fermentation device and the dry fermentation method to prepare the biogas through the high-concentration solid raw materials become an important choice for efficiently (high content of solid) processing fermentable organic waste in the fields of industry and agriculture.

The invention provides a breeding method for a liquid strain of morchella, and also provides an industrial cultivation method for the morchella. The breeding method for the liquid strain of the morchella comprises the following steps: firstly preparing a first culture solution from potato, sawdust, water, glucose, magnesium sulfate, soil and agar, and putting a tissue of the joint of a stipe and a cap of the morchella in the first culture solution for culture to obtain a mother strain; then, preparing a second culture solution from the potato, the sawdust, the water, the glucose, the magnesium sulfate, the soil and vitamin B1, distributing the second culture solution into shake flasks, putting the mother strain in the shake flasks, and culturing to obtain a first liquid strain. The industrial cultivation method for the morchella mainly comprises the following steps: firstly performing inoculated culture on the first liquid strain to obtain a fungi bag, and then cultivating the fungi bag in soil. The industrial cultivation method for the morchella has the advantages of being short in production cycle, simple, low in infection rate, and the like.

The invention discloses a mixed culture material for cultivation of eryngii mushroom. The mixed culture material consists of the following raw materials in percentage by weight in drying states: 10-20% of weed tree sawdust, 35-40% of corncob, 10-20% of bagasse, 15-25% of wheat bran, 3-8% of corn meal, 2-7% of soybean meal, 1-5% of calcium carbonate and 1-5% of lime; the water content of the mixed culture material after pre-wetting is 65+/-1%, and the pH value is 7-9. The invention also designs a method for bagging the mixed culture material for cultivation of eryngii mushroom. The mixed culture material for cultivation of eryngii mushroom and the bagging method of the mixed culture material, designed by the invention, can be used for ensuring the production speed and color and luster of eryngii mushroom, improving the nutritive values of eryngii mushroom, reducing the cost and improving the yield.

A preparation method of porcine reproductive and respiratory syndrome vaccines by utilizing a bioreactor utilizes a stream perfusion type bioreactor as a cultivation tool. The preparation method comprises the steps of a cultivating cells for preparing the vaccines; b vaccinating and cultivating viruses; c harvesting virus liquor; d preparing the vaccines and the like. The preparation method has the advantages that the preparation method is simple and convenient in operation, low in contamination probability and capable of remarkably reducing preparation cost, the vaccines are large in yield, uniform and stable in quality and good in immune effect, and the like. The whole preparation process does not involve other problems of biological safety and public hygiene so that the preparation method is suitable for large-scale preparation of the porcine reproductive and respiratory syndrome vaccines.

The invention provides a liquid strain breeding method of the albumen fungus, and also provides a field bionic cultivation method of the albumen fungus. The liquid strain breeding method of Gallus firsum is: earlier use potato, sawdust, corncob powder, water, glucose, magnesium sulfate, potassium dihydrogen phosphate, peptone, vitamin B1, yeast sheet and agar to make the first nutrient solution, the The tissue at the junction of the stipe and cap of Gallus ciliariae is put into the first culture medium and cultivated to obtain the mother species; then use potatoes, sawdust, corncob powder, water, glucose, magnesium sulfate, potassium dihydrogen phosphate, peptone and Vitamin B1 is used to prepare the second culture solution, and the second culture solution is divided into shake flasks, the mother species is inserted into the shake flask, and the first liquid strain is obtained after cultivation. The biomimetic cultivation method of field field of gallinaceous fungus is mainly to first inoculate and cultivate the first liquid strain to obtain the bacterial bag, and then cultivate the bacterial bag into the soil. The field biomimetic cultivation method of Gallus fruticosa has the advantages of short production cycle, simple method and low infection rate.

The invention belongs to the field of solid waste disposal and resource utilization, and in particular relates to a process for production of biogas by wet method-dry method combined two-stage anaerobic fermentation. Broken solid waste and an inoculum are mixed in a full wet type reactor for wet anaerobic fermentation, the wet anaerobic fermentation period hydraulic retention time (HRT) is 5-20 days, after fermentation processing, a fermentation material can be delivered to a dry method fermentation reactor with an infiltration device at the bottom for dry solid fermentation for production of the biogas, and the process combines the dual advantages of wet method fermentation and dry method fermentation. The fermentation process integrating the advantages of fast mass transfer and easy start-up characteristics of the wet method fermentation and reaction space saving of the dry method fermentation, and the like is disclosed. By combination of biomass multistage decomposition and transformation in the fermentation process and methane producing bacteria group enrichment principle, the wet fermentation process advantage is used, the wet fermentation process is used as a homogeneous inoculation stage of the dry method fermentation process, and the problems of difficult inoculation and slow start of the dry method fermentation can be solved.

The invention discloses a high water holding capacity pleurotus eryngii mixed compost, which comprises raw materials with percentage by weight in a dried state: poplar sawdust 5-10%, weedtree barks 5-10%, corn cobs 35-40%, bagasse 10-20%, bran 15-25%, corn flour 3-8%, soyabean 2-7%, calcium carbonate 1-5% and lime 1-5%, wherein the water content of the high water holding capacity pleurotus eryngii mixed compost is 65%+_1, and the potential of hydrogen (pH) of the high water holding capacity pleurotus eryngii mixed compost is 7-9. The invention further discloses a method for bagging the high water holding capacity pleurotus eryngii mixed compost. The high water holding capacity pleurotus eryngii mixed compost and the bagging method thereof can guarantee production speed and color of pleurotus eryngii, effectively improve yield of the pleurotus eryngii, effectively reduce cost, and guarantee nutrition and quality of the pleurotus eryngii simultaneously.

The invention discloses an edible fungus liquid inoculating device, comprising a rack and a horizontal transport mechanism arranged on the rack for transporting a tray, and the horizontal transport mechanism is provided with a plurality of material-blocking mechanisms for blocking the movement of the tray along the transport direction of the tray at intervals; a positioning mechanism for clampinga culture container body in the tray is arranged above the material-blocking mechanism at the most front end of the rack; and the device further comprises a cover-opening mechanism for opening a bottle cover and an inoculation pipeline mechanism for conducting uniform inoculation for all culture containers in the tray. When the material-blocking mechanism at the most front end of the horizontal transport mechanism along the transport direction blocks a cargo, a detection signal is generated, and the detection signal is transmitted to a control device; and the control device sequentially controls the positioning mechanism, the cover-opening mechanism and the inoculation pipeline mechanism in response to the detection signal. The edible fungus liquid inoculation device can effectively prevent invasion of planktonic bacteria, and is high in automation degree, high in efficiency and uniform in inoculation.

The invention discloses a method for propagating insects and a device special for propagating the insects. A floating device comprises a floating plate with a through hole, and a pipe, and the pipe is connected with the floating plate through the through hole; a breeding pool is divided into four areas, each area is isolated by using a baffle and a net cover, the baffle is positioned in the breeding pool, and the net cover covers above each area; and a door through which the floating device passes is arranged on the baffle, and the aperture of the net cover ensures that the insects cannot pass through the net cover. When the insects are propagated by using the device, single plants can float in a nutrient solution, the single plant control of plants can be realized, and the adjustment can also be made by controlling the positions of the single plants in a culture pond even under the condition that the inoculation and culture are uniform, so that the insects on each plant are consistent in propagation speed and uniform in quantity and quality, the propagation speed, the quantity and the quality can meet production requirements, the device can be operated more flexibly and conveniently, and the waste of space, manpower and material resources is avoided. The method and the device can be popularized and applied in the industry.

The invention relates to an automatic koji inoculating machine which comprises a tank. The tank is provided with a strain culture cavity, a container with a culture base material laid in is arranged in the strain culture cavity, the tank is further provided with a sterilizing mechanism and a negative-pressure inoculating mechanism, the sterilizing mechanism comprises an air flow incoming passage and an air flow outgoing passage, one end of the air flow incoming passage is communicated with the strain culture cavity while the other end of the same is communicated with a steam supply source and a sterile air source and can switch between the two, and one end of the air flow outgoing passage is communicated with the strain culture cavity while the other end of the same is communicated with an air liquid collection source; the negative-pressure inoculating mechanism comprises an inoculating pipe, an inoculating tank and a negative-pressure passage, the inoculating pipe and the negative-pressure passage are positioned on two sides of the tank, the inoculating pipe communicates the inoculating tank with the strain culture cavity and is provided with a an inoculating control valve for controlling on-off of the inoculating pipe, and one end of the negative-pressure passage is communicated with the strain culture cavity while the other end of the same is communicated with a negative-pressure generator. By adopting the above scheme, the automatic koji inoculating machine is good in sterilizing effect and quick and uniform in inoculating.

The present invention provides a method and a device for two-stage dry digestion of wastes. The method comprises: inoculating microorganisms in a first-stage reactor, to make waste materials fully fermented to produce acid; after acid production is finished, using alkaline substances to neutralize organic acid so as to regulate the pH value; and performing a second stage of dry fermentation to produce methane. The method can effectively overcome suppression of organic acid to a digestion process during anaerobic digestion, and does not produce a large amount of waste water because rinsing is not required.

The invention provides a flow production line of edible fungi cultivation. Based on poverty alleviation with projects of mountainous rural areas, by means of the characteristics that edible fungi grow fast and the growth period is short, the flow production line of edible fungi cultivation which brings convenience to development on large scale is designed. The flow production line of edible fungi cultivation mainly comprises the steps that (1) stacking fermentation of raw materials of edible fungi cultivation is carried out, microbial populations of medium raw materials are controlled, and the utilization of nutrient substances of the medium materials is improved; (2) high-temperature short-time sterilization is carried out, and by means of tunnel sterilization equipment, flow-line sterilization is conducted on the prepared medium materials; (3) a liquid strain converted from an inoculated strain through fermentor culture is directly sprayed to a sterilized medium, so that integration of medium sterilization-inoculation of edible fungi cultivation is achieved, and the production efficiency is improved; (4) traditional small fungi bags are no longer used in cultivation, fungi bags with large volumes are used, and after primary sterilization of the fungi bags is carried out, fungi materials to be cultured are directly put in the fungi bags in the condition that large-area opening is not conducted.

The invention discloses a shaking table used for various adherent cell bed board and drug treatment tests and application. The shaking table comprises four parts: the first part is located below, is the shaking table body, is provided with a mode regulating knob, a temperature regulating knob, a speed regulating knob and a time regulating knob, has two power supply modes, namely a storage batteryand a socket, and meanwhile, is provided with a timed alarm device; a second part is located above, is a metal device capable of performing heat conduction, is provided with a cell culture dish, has groove constant-temperature holes targeted to cell culture dishes and cell culture plates, and has a circle of groove constant-temperature holes targeted to a sterile hood at the circumference, so as to be mutually buckled with a third part; the fourth part is located uppermost, is the sterile hood, and is mutually buckled with the third part. The shaking table is simple and reliable in assembly, and simple and convenient to operate; uniform distribution of cells and drugs is realized by shaking culture dishes and culture bottles with manpower in the prior art, while the shaking table disclosedby the invention can avoid the problems that pollutant cells are shaken out from the culture medium, the cell inoculum density is non-uniform, and drug distribution is non-uniform, caused by a conventional manual method.

The invention discloses a continuous dry digestion device for wastes. The continuous digestion device comprises a reactor, wherein a compound stirring system is arranged in the reaction cavity of thereactor; the compound stirring system comprises a stirring shaft; a plurality of flat-paddle type stirring paddles are distributed on the stirring shaft along an axial direction, and two adjacent flat-paddle type stirring paddles are distributed along opposite directions; a paddle is fixedly arranged between two adjacent flat-paddle type stirring paddles; the vane of the paddle extends along the axial direction of the stirring shaft and forms an included angle with the stirring shaft. Through the dry digestion of microbial fermentation, the operation cost of a dry digestion process can be greatly lowered, and in addition, digestion reaction can be accelerated so as to be favorable for improving the yield of methane. The invention also discloses a continuous dry digestion method for wastes.

The invention discloses an identification method for resisting hard smut of highland barley. The identification method comprises the following steps: step 1, collecting teliospores: collecting diseased ears from a heading stage to a filling and milk-ripe stage of the highland barley, drying and storing; step 2, preparing teliospore suspension liquid: crushing the teliospores in step 1 and sievingto obtain teliospore powder; preparing suspension liquid and filtering to obtain the needed suspension liquid; step 3, inoculating bacteria: treating seeds by utilizing the suspension liquid in step 2through a vacuum permeation method; step 4, drying the seeds in step 3 in the shade and sowing; and step 5, identifying a disease-resisting level according to a disease-resisting grading standard. According to the bacteria inoculating method disclosed by the invention, highland barley hard smut bacteria can be accurately inoculated onto the highland barley seeds to induce the hard smut of the highland barley; the identification method can be used for identifying the hard smut resistance of highland barley varieties or resource materials; and the method is simple and feasible, high in accuracyand strong in operability.

The invention belongs to the technical field of biological medicine, and relates to an animal-derived-component-free passaging method for passaging of stem cells, in particular to preparation of animal-derived-component-free digestive juice and digestive stopping solution for clinical-grade stem cells and an animal-derived-component-free passaging method for passaging of the stem cells. The animal-derived-component-free passaging method for passaging of the clinical-grade stem cells comprises the following steps: extracting human amniotic membrane and umbilical cord mesenchymal stem cells, performing routine culture to a third generation, and carrying out cell identification; inoculating the third-generation human amniotic membrane and umbilical cord mesenchymal stem cells into a 75cm<2> culture bottle for culture until the convergence degree reaches 80-90%; and making the human amniotic membrane and umbilical cord mesenchymal stem cells passage by applying animal-derived-component-free digestive juice and digestive stopping solution. The animal-derived-component-free passaging method for passaging of the stem cells provided by the invention not only meets the animal-derived-component-free standard, but also can effectively digest the stem cells to unicellular morphology, and thereby the stem cells are uniformly inoculated, and the optimal culture state is achieved.

The invention discloses a liquid inoculation device for edible fungi. The solid inoculation device comprises a frame, and a horizontal transport mechanism arranged on the frame and used for transporting a tray, wherein the horizontal transport mechanism is provided with a plurality of material blocking mechanisms used for blocking movement of the tray along the transport direction of the tray at intervals; a positioning mechanism used for clamping the bottle bodies of culture containers in the tray is arranged above a material blocking mechanism located at the most front end of the frame; thesolid inoculation device also comprises a cap opening mechanism used for opening bottle caps and an inoculation pipeline mechanism capable of realizing simultaneous and uniform inoculation of all culture containers in the tray; when the material blocking mechanism located at the most front end along the transport direction of the horizontal transport mechanism blocks goods, a detection signal is generated and transmitted to a control unit; and the control unit responds to the signal to successively control the positioning mechanism, the cap opening mechanism and the inoculation pipeline mechanism. The liquid inoculation device for the edible fungi provided by the invention can effectively prevent invasion of planktonic bacteria and has the advantages of high degree of automation, high efficiency and inoculation uniformity.

The invention discloses a rice seedling planting device for rice blast resistance identification, the rice seedling planting device comprises a base, motors, screw rods, electric motors, water valvesand spray heads, wherein the motors are arranged in the middle of the interior of the base, the output ends of the motors are connected with the screw rods, the top ends of the screw rods are connected with a top plate through bearings, planting tanks are formed in the two sides of the upper portions of planting trays, one sides of the planting tanks are connected with connecting blocks, one sidesof the connecting blocks are connected with meshing gear blocks, the lower portion of a fixed water pipe is connected with a connecting water pipe, the water valves are arranged above the connectingwater pipe, and the spray heads are uniformly distributed at the bottom of the connecting water pipe. The rice seedling planting device for rice blast resistance identification is applied to the riceseedling planting, and the whole device can well ensure that rice is uniformly inoculated to against rice blast, the occupied space is small, and the occurrence rate of errors in the acquisition process of data is small.