Identification method for resisting hard smut of highland barley

Abstract

The invention discloses an identification method for resisting hard smut of highland barley. The identification method comprises the following steps: step 1, collecting teliospores: collecting diseased ears from a heading stage to a filling and milk-ripe stage of the highland barley, drying and storing; step 2, preparing teliospore suspension liquid: crushing the teliospores in step 1 and sievingto obtain teliospore powder; preparing suspension liquid and filtering to obtain the needed suspension liquid; step 3, inoculating bacteria: treating seeds by utilizing the suspension liquid in step 2through a vacuum permeation method; step 4, drying the seeds in step 3 in the shade and sowing; and step 5, identifying a disease-resisting level according to a disease-resisting grading standard. According to the bacteria inoculating method disclosed by the invention, highland barley hard smut bacteria can be accurately inoculated onto the highland barley seeds to induce the hard smut of the highland barley; the identification method can be used for identifying the hard smut resistance of highland barley varieties or resource materials; and the method is simple and feasible, high in accuracyand strong in operability.

Description

technical field [0001] The invention relates to the technical field of identification of highland barley disease resistance, in particular to an identification method for highland barley resistance to hard smut. Background technique [0002] Stem smut is a common seed-borne fungal disease of Tibetan highland barley, and it occurs to varying degrees in Tibetan highland barley planting areas. The incidence rate of the general plot is 4% to 5%, resulting in an average yield loss of 1% to 5%. General control methods include establishing disease-free farms, producing disease-free seeds, excavating disease-resistant resources, cultivating disease-resistant varieties, reasonable crop rotation, seed coating treatment, timely removal of diseased plants, centralized deep burial or incineration, etc. [0003] Breeding hard smut-resistant varieties is the most economical and effective way to control the disease, and the identification of the source of resistance is the basis of disease...

AI Technical Summary

Problems solved by technology

However, there are few studies on the identification of resistance of highland barley varieties to smut in China at present.

Mainly adopt the field planting identification method (establish a smut identification nursery in barley smut, sow barley material identification materials in rows, through natural induction, investigate the incidence of highland barley at the earing stage, and use the classification method for resistance classification and grading) According to the susceptibility rate of highland barley, the classification method is used to classify and classify the resistance. The current method is greatly affected by the interaction of the environment, and the seeds carry little or no bacteria. The results of field identification and resistance have a large difference. Accurately reflect the disease resistance of varieties, etc.

Images