The present invention discloses a quick, simple, qualitative and semi-quantitative immunologic method and device in a field test kit format for the detection and the stage of infection of certain protozoal, viral, bacterial, or nematodal pathogens in body fluids, using vaccines specific to such pathogens as antigens. The method comprises immobilizing an
antigen specific to a selected protozoal, bacterial, viral or nematodal
pathogen on a support membrane, obtaining a sample of a
body fluid from a human or animal suspected of harboring the
pathogen, thus containing antibodies thereto, contacting the
antigen with the sample of
body fluid and detecting the binding of the
antigen with the antibodies by any suitable means. The device comprises a rigid member, an absorbent layer, a foam layer and a support member on which the antigen is immobilized, glued together to form a compact
assembly. The field test kit comprises the device enclosed in a casing and vials containing additional reagents such as washing and
dilution buffers, a
chromogenic label for the detection of the antigen-
antibody complex and positive and
negative control solutions to verify the accuracy of the tests,
instructions for use and the like. All the components are packaged in a small plastic container or box for easy transportation and use. While this invention is particularly adaptable to the detection of S. neurona using its vaccine as an antigen, other pathogens can also be detected by the method of this invention. These include but are not limited to Neospora hughesi, Neospora Canninum,
Leishmania,
Leptospira,
West Nile virus, and other similar organisms.