17 results about "Megalobrama" patented technology

The present invention belongs to the field of aquatic biological breeding, and specifically relates to a method and application of creating germplasm without intermuscular spines of economic fish for breeding. runx2b The economic fish, Megalobrama amblycephala, is gene-edited using a gene as the target gene. F0 generation fish with mutations are selected and cultured as parent fish. The F1 generation is produced by mating and breeding the F0 generation mutant male and female parents. F1 fish without intermuscular spines are screened and cultured to adult fish for use in cultivating a new germplasm of Megalobrama amblycephala without intermuscular spines. The present invention utilizes a gene mutation method to obtain a new germplasm of the economically cultivated fish, Megalobrama amblycephala, without intermuscular spines. This provides an improved method for solving the problem of the significant impact of intermuscular spines in the muscles of important aquacultured economic fish such as silver carp, bighead carp, carp, crucian carp, and bream in my country on their consumption and processing. It also provides a basis for subsequent research on the molecular formation mechanism of intermuscular spines, and has important scientific research value and industrial application value.

The invention discloses SSR (Simple Sequence Repeat) multiple PCR (Polymerase Chain Reaction) primers for megalobrama amblycephala paternity test and application, the primers comprise seven pairs of specific primers, namely a primer pair MamGLB2501, a primer pair MamGLB2502, a primer pair MamGLB2503, a primer pair MamGLG2504, a primer pair MamGLG2505, a primer pair MamGLR2506 and a primer pair MamGLR2507, and the base sequences of the seven pairs of specific primers are sequentially shown as SEQ ID NO.1-14; the invention also discloses an SSR multiplex fluorescent PCR method for megalobrama amblycephala paternity test by using the primer and application of the primer or the method in megalobrama amblycephala paternity test. The microsatellite site amplified by the primer is 3-6 base repetition, the typing is accurate, and the polymorphism of the amplified site is high; the method is accurate in identification and low in price.

The invention discloses application of a functional gene ghrb recombinant variation molecular marker in breeding of hybrid megalobrama amblycephala, and the functional gene ghrb recombinant variation molecular marker is used for identifying germplasm of the hybrid megalobrama amblycephala or screening backcross filial generation of the hybrid megalobrama amblycephala with fast growth character. Wherein the functional gene ghrb recombinant variation molecular marker is obtained by mutation of bases located at the 2079th site, the 2083th site, the 2101th site and the 2161th site of the functional gene ghrb, the base at the 2079th site is C or A, the base at the 2083th site is A or G, the base at the 2101th site is C or A, and the base at the 2161th site is G or C. According to the functional gene ghrb recombinant variation molecular marker, the germplasm of the hybrid megalobrama amblycephala can be rapidly identified, the hybrid megalobrama amblycephala backcross filial generation with the rapid growth character can be accurately screened out, technical support can be provided for accurate cross breeding, and screening sites can be provided for development of breeding chips.

The application provides a seven-day period factory cyclic Megalobrama amblycephala fry behavior domestication method and system, relates to the Megalobrama amblycephala breeding technical field, and includes: domesticating the fry into a domestication pond on the first day; starting vertical water flow impact before feeding fresh bait fish according to a preset first feeding frequency on the second day; feeding electric shock disabled live bait according to a preset second feeding frequency on the third day; feeding mixed live bait according to a preset third feeding frequency on the fourth day; feeding mosaic bait according to a preset fourth feeding frequency on the fifth day; feeding pure artificial compound feed using a composite feeding mode and starting water flow impact on the sixth day; feeding pure artificial compound feed on the seventh day, and screening domestication successful individuals based on behavior indexes after the period ends, so that the Megalobrama amblycephala is rapidly domesticated from feeding live bait to feeding artificial compound feed.

The invention relates to the field of fishery medicines, and discloses a megalobrama amblycephala gonadotropin releasing hormone analogue, a composite oxytocic and application. The hormone analogue MaGnRH3a provided by the invention has a remarkable effect of promoting megalobrama amblycephala egg cell maturation. The effect time of the female parent fish to be parturified by brood on the MaGnRH3a oxytocic is short, and the parturition induction efficiency is high. The modified and designed MaGnRH3a is an efficient active peptide specifically aiming at artificial spawning induction of megalobrama amblycephala. The ovulation time can be shortened to 10-13 hours and is increased by 41% compared with that of a traditional method, and the death rate of parent fishes is reduced to 0% from 16%; and through specific design, the endocrine interference risk of non-target organisms is remarkably reduced. When the active peptide and domperidone are compounded into an oxytocic, the compound oxytocic has obvious economic and ecological benefits when being applied to megalobrama amblycephala artificial breeding production.

The application discloses a kind of Megalobrama amblycephala parentage identification SSR multiplex PCR primer and application, the primer includes 7 pairs of specific primers, respectively primer pair MamGLB2501, MamGLB2502, MamGLB2503, MamGLG2504, MamGLG2505, MamGLR2506 and MamGLR2507, the base sequence of 7 pairs of specific primers is sequentially shown as SEQ ID NO.1~14;Also disclosed is the SSR multiplex fluorescence PCR method for Megalobrama amblycephala parentage identification using the above primer, and the application of the above primer or method in Megalobrama amblycephala parentage identification.The microsatellite site amplified by the primer of the application is 3-6 base repeats, accurate in typing, and high in amplification site polymorphism;The method of the application is accurate in identification and low in price.

The invention discloses a PCR (Polymerase Chain Reaction) primer group, kit and method for rapidly identifying parabramis pekinensis, megalobrama amblycephala and hybrids thereof, and particularly belongs to the technical field of molecular biology identification, the primer group comprises a forward primer F and a reverse primer R. The nucleotide sequence of the forward primer F is shown as SEQ ID No: 1, and the nucleotide sequence of the reverse primer R is shown as SEQ ID No: 2. Through the kit and the method designed by the PCR primer group, rapid identification of parabramis pekinensis, megalobrama amblycephala and hybrids thereof is realized.

The invention discloses an application of a haplotype of a megalobrama amblycephala body weight associated SNP site in breeding, and belongs to the technical field of aquatic breeding. The haplotype is randomly formed by a haplotype I, a haplotype II and a haplotype III, basic groups at positions 457 and 770 of a myosin IB gene of the haplotype I are A and G respectively, basic groups at positions 457 and 770 of the myosin IB gene of the haplotype II are A and C respectively, basic groups at positions 457 and 770 of the myosin IB gene of the haplotype III are T, C, T and C respectively, and basic groups at positions 770 and 770 of the myosin IB gene of the haplotype III are A and G respectively. Compared with other diplotypes, the homozygous double V type randomly formed by the haplotype III has remarkable advantages in average weight and myosin IB gene expression, and can be used for auxiliary breeding of megalobrama amblycephala fast-growing new line molecules.

The invention discloses a device and a method for measuring residual feed intake of megalobrama amblycephala based on image processing, which are characterized in that juvenile megalobrama amblycephala is independently fed by an isolated cage culture method, and high-precision batch analysis of feed counting is realized by combining Image J software for the first time. The technical problems that the food consumption character of megalobrama amblycephala is difficult to measure and the water quality is difficult to regulate and control through independent feeding are solved. According to the method, the fish body cannot be damaged in the measurement process, the water body replacement efficiency is improved, the stress reaction of the fish caused by water body replacement is effectively reduced, in addition, the method is convenient to manufacture, low in cost and low in site requirement, individual feed intake data of megalobrama amblycephala can be accurately and rapidly obtained, phenotype data of remaining feed intake characters of megalobrama amblycephala can be obtained, and the method is suitable for popularization and application. And a foundation is laid for subsequent genetic evaluation and excellent new product breeding of the economic character.

The invention discloses a molecular marker, a primer pair and a kit for identifying megalobrama amblycephala-induced red crucian carp gynogenesis progeny as well as application and a method of the molecular marker. The nucleotide sequence of the molecular marker is shown as SEQ ID NO.1. The invention further discloses a method for identifying megalobrama amblycephala-induced red crucian carp gynogenesis progeny. Based on re-sequencing analysis and genome comparison of megalobrama amblycephala-induced gynogenesis progeny of the red crucian carp, a male parent gene segment from megalobrama amblycephala is screened, and a primer pair with megalobrama amblycephala molecular marker and specificity is developed, so that the megalobrama amblycephala-induced gynogenesis progeny of the red crucian carp can be effectively identified, and the application prospect of the megalobrama amblycephala-induced gynogenesis progeny is broad. The method effectively avoids the problem that identification is difficult due to the fact that appearance is difficult to distinguish, is simple, rapid and high in accuracy, and can play an important role in the field of fish genetic breeding.

The invention provides a seven-day-cycle factory circulating water mandarin fish fry behavior domestication method and system, and relates to the technical field of mandarin fish culture. On the second day of domestication, vertical water flow impact is started according to the preset first feeding frequency before fresh bait fishes are fed; on the third day of domestication, feeding live baits disable by electric shock according to a preset second feeding frequency; feeding mixed live baits according to a preset third feeding frequency on the fourth day of domestication; on the fifth day of domestication, the inlaid bait is fed according to the preset fourth feeding frequency; on the sixth day of domestication, pure artificial compound feed is fed in a compound feeding mode, and water flow impact is started; pure artificial compound feed is fed on the seventh day of domestication, and successfully domesticated individuals are screened based on behavior indexes after the period is ended, so that rapid domestication of the mandarin fish from ingestion of live baits to ingestion of the artificial compound feed is realized.

The application discloses a method for culturing and screening of zygotes of Megalobrama amblycephala, and steps include: S1, culturing parent fish in a light-proof incubator; S2, a conveying belt is used for receiving and conveying the discharged zygotes; S3, automatic screening is performed according to the characteristics of live eggs and dead eggs, and a collecting assembly is used for cleaning and collecting the live eggs with sticky characteristics; S4, a piston plate reciprocates in a piston cylinder, gas is discharged to the surface of the conveying belt through a bubble tube, and secondary screening is performed on the zygotes; and S5, a piston rod moves to drive a regulating assembly to work through a friction plate, so that the movement speed of the collecting assembly is adjusted. Through the automatic screening of the characteristics of the live eggs and the dead eggs in the conveying process, and the secondary screening of the gas discharged to the surface of the conveying belt through the bubble tube when the conveying assembly works, the piston rod drives the piston plate to reciprocate in the piston cylinder, the dead eggs can be effectively removed, and the overall fertilization rate of the zygotes is improved.

The present application belongs to the field of fish distant hybrid breeding, and discloses a method for distant hybridization between Megalobrama amblycephala and the genus Procyprinus. Megalobrama amblycephala and Procyprinus are selected as parent fish to artificially induce spawning, and are transferred to an ecological breeding net cage to naturally spawn and fertilize, and to flow water natural incubation. For parent fish that fail to successfully spawn after the effect period of the induced spawning drug, artificial dry insemination is adopted, and static water incubation is carried out. After the hatched fry are hatched and all have a waist point, feeding is started, and then the diploid hybrid offspring of Megalobrama amblycephala and Procyprinus is obtained. The hybridization method of the present application is to use Megalobrama amblycephala and Procyprinus for intergeneric distant hybridization, successfully combines the high back, fast growth of Megalobrama amblycephala and the slender body shape, tender meat quality, and good stress resistance of Procyprinus, and creatively breeds a new type of Megalobrama amblycephala and Procyprinus diploid hybrid fish with long body shape, wide back, better muscle quality, and stronger stress resistance, which is a new type of distant hybridization.

This invention discloses a whole-genome liquid-phase microarray of blunt snout bream, its preparation method, and its applications. The microarray contains 49,173 highly polymorphic core SNP molecular markers, the loci of which were determined by sequence alignment with reference to the blunt snout bream genome ASM1881202v1. The whole-genome liquid-phase microarray of blunt snout bream prepared in this invention selects characteristic SNP loci from three major wild populations and three new varieties, ensuring that the loci are evenly distributed across the genome and comprehensively cover core variation information under different genetic backgrounds, giving the markers broad universality and effectiveness. Simultaneously, the microarray includes loci significantly associated with important economic traits such as growth, disease resistance, and hypoxia tolerance, closely integrating molecular markers with actual production needs. It can not only be used to detect the genotype of blunt snout bream DNA samples but also directly serve population cluster analysis and the breeding of disease-resistant new varieties, significantly enhancing the practical value of the microarray in blunt snout bream breeding practice.

The application discloses a PCR primer group, a kit and a method for rapidly identifying Siniperca jenkinsii, Megalobrama amblycephala and their hybrid, and belongs to the technical field of molecular biology identification, wherein the primer group comprises a forward primer F and a reverse primer R, the nucleotide sequence of the forward primer F is shown as SEQ ID No:1, and the nucleotide sequence of the reverse primer R is shown as SEQ ID No:2. Through the kit and the method designed by the PCR primer group, rapid identification of Siniperca jenkinsii, Megalobrama amblycephala and their hybrid is realized.

The invention belongs to the technical field of feed, and particularly relates to a compound Chinese herbal medicine feed additive for promoting growth of megalobrama amblycephala and improving immunity and application thereof. The invention discloses a compound Chinese herbal medicine feed additive which is prepared from the following components in parts by weight: 3-6 parts of radix astragali, 2-4 parts of fructus lycii, 1-2 parts of radix ginseng and 0.5-1.5 parts of rhizoma phragmitis. The compound Chinese herbal medicine feed additive can improve the survival rate of fishes, promote the growth of the fishes and improve the immunity and disease resistance of the fishes through the synergistic effect of all the components. The compound Chinese herbal medicine feed additive can be used for preparing fish feed, and experiments prove that after the compound Chinese herbal medicine feed additive is added into the fish feed, the growth of megalobrama amblycephala can be promoted, and the immunity and digestive function of the megalobrama amblycephala can be improved.

The present invention relates to the field of genetic engineering technology, and in particular to a gene editing method and application for constructing hypoxia-resistant amblycephalopod by targeting the mylipb gene. The present invention provides a gRNA targeting the mylipb gene, and a composition composed of the gRNA and the Cas9 protein, which can be used to knock out the mylipb gene. The present invention also provides a gene editing method targeting the mylipb gene of amblycephalopod based on the CRISPR / Cas9 system, comprising designing a target sequence, preparing a gRNA, and microinjecting the composition of the gRNA and the Cas9 protein into fertilized eggs in vitro to obtain F0 generation mutants. The F0 generation is self-pollinated to obtain the F1 generation, and gene-knockout heterozygous amblycephalopod is obtained by genotyping. The heterozygous amblycephalopod is phenotypically identified as a germplasm material that is more resistant to hypoxia. The breeding method provided by the present invention has significant advantages over traditional methods, realizes rapid and accurate directional transformation of biological traits, greatly shortens the breeding cycle, improves breeding efficiency, and has important application value.