4 results about "Megalobrama" patented technology

The application provides a seven-day period factory cyclic Megalobrama amblycephala fry behavior domestication method and system, relates to the Megalobrama amblycephala breeding technical field, and includes: domesticating the fry into a domestication pond on the first day; starting vertical water flow impact before feeding fresh bait fish according to a preset first feeding frequency on the second day; feeding electric shock disabled live bait according to a preset second feeding frequency on the third day; feeding mixed live bait according to a preset third feeding frequency on the fourth day; feeding mosaic bait according to a preset fourth feeding frequency on the fifth day; feeding pure artificial compound feed using a composite feeding mode and starting water flow impact on the sixth day; feeding pure artificial compound feed on the seventh day, and screening domestication successful individuals based on behavior indexes after the period ends, so that the Megalobrama amblycephala is rapidly domesticated from feeding live bait to feeding artificial compound feed.

The application discloses a kind of Megalobrama amblycephala parentage identification SSR multiplex PCR primer and application, the primer includes 7 pairs of specific primers, respectively primer pair MamGLB2501, MamGLB2502, MamGLB2503, MamGLG2504, MamGLG2505, MamGLR2506 and MamGLR2507, the base sequence of 7 pairs of specific primers is sequentially shown as SEQ ID NO.1~14;Also disclosed is the SSR multiplex fluorescence PCR method for Megalobrama amblycephala parentage identification using the above primer, and the application of the above primer or method in Megalobrama amblycephala parentage identification.The microsatellite site amplified by the primer of the application is 3-6 base repeats, accurate in typing, and high in amplification site polymorphism;The method of the application is accurate in identification and low in price.

The invention provides a seven-day-cycle factory circulating water mandarin fish fry behavior domestication method and system, and relates to the technical field of mandarin fish culture. On the second day of domestication, vertical water flow impact is started according to the preset first feeding frequency before fresh bait fishes are fed; on the third day of domestication, feeding live baits disable by electric shock according to a preset second feeding frequency; feeding mixed live baits according to a preset third feeding frequency on the fourth day of domestication; on the fifth day of domestication, the inlaid bait is fed according to the preset fourth feeding frequency; on the sixth day of domestication, pure artificial compound feed is fed in a compound feeding mode, and water flow impact is started; pure artificial compound feed is fed on the seventh day of domestication, and successfully domesticated individuals are screened based on behavior indexes after the period is ended, so that rapid domestication of the mandarin fish from ingestion of live baits to ingestion of the artificial compound feed is realized.

This invention discloses a whole-genome liquid-phase microarray of blunt snout bream, its preparation method, and its applications. The microarray contains 49,173 highly polymorphic core SNP molecular markers, the loci of which were determined by sequence alignment with reference to the blunt snout bream genome ASM1881202v1. The whole-genome liquid-phase microarray of blunt snout bream prepared in this invention selects characteristic SNP loci from three major wild populations and three new varieties, ensuring that the loci are evenly distributed across the genome and comprehensively cover core variation information under different genetic backgrounds, giving the markers broad universality and effectiveness. Simultaneously, the microarray includes loci significantly associated with important economic traits such as growth, disease resistance, and hypoxia tolerance, closely integrating molecular markers with actual production needs. It can not only be used to detect the genotype of blunt snout bream DNA samples but also directly serve population cluster analysis and the breeding of disease-resistant new varieties, significantly enhancing the practical value of the microarray in blunt snout bream breeding practice.