The invention discloses an insulin carrying microsphere. A polyesteramide material of which the side chain contains a carboxyl and a hydrophobic isobutyl group at the same time serves as a carrier, wherein the carboxyl endows entericsolubility to the insulin carrying microsphere. Under a strong-acidity environment of gastric juice, because of deprotonation of the carboxyl, the polyesteramide material is not dissolved, the microsphere has certain contractile effect, and then a protein medicament is protected. Under a neutral condition of a small intestine, because of ionization of the carboxyl, the polyesteramide material is gradually dissolved, the microsphere is corroded, and then the wrapped medicament is released. On the other hand, the hydrophobic isobutyl has the effect of regulatingthe pH sensitivity of the material, the hydrophobicity of the insulin carrying microsphere is improved, and the effect with an intestinal cell membrane is enhanced. Therefore, the insulin carrying microsphere has high pH sensitivity, and the releasing and absorption of the insulin in an intestinal tract can be realized.