79 results about "Intestinal cell" patented technology

The invention belongs to the technical field of biology, and discloses a reagent combination or kit for constructing intestinal organs and application of the reagent combination or kit. According to the reagent combination or the kit, intestinal organs can be obtained from cell-derived epithelial cells obtained from a donor in a non-invasive manner, and the obtained intestinal organs can be cryopreserved and recovered and can be amplified in vitro for a long time; transcriptome characteristics are similar to those of real human small intestine tissues, and typical marker genes of various small intestine pedigree cell types are highly expressed; compared with intestinal organs obtained through induction of pluripotent stem cells, the intestinal organs have more intestinal pedigree characteristics, and the intestinal function development is more mature; after being promoted to be mature, the intestinal organ also highly expresses genes related to drug absorption and metabolism, has drug absorption capability similar to that of an immortalized intestinal cell line, but more prominently shows intestinal cell lineage characteristics, is closer to an intestinal environment in a real human body, and can be used for screening intestinal disease drugs; the intestinal barrier function is realized.

The invention discloses a prebiotic-loaded intestinal targeting exosome and a preparation method and application thereof.The preparation method comprises the steps that firstly, plasmids containing intestinal cell targeting peptide sequences are constructed through a gene editing technology, the plasmids are transfected and introduced into cells, the transfected cells are screened with antibiotics, and cell strains stably expressing intestinal cell targeting peptides are obtained; then culturing and adding prebiotics, and continuously culturing to enable the cells to take in the prebiotics and release the exosomes loaded with the prebiotics; and finally, collecting cell culture supernate, and centrifuging and resuspending for multiple times under a low-temperature condition to obtain the prebiotics-loaded intestinal targeting exosome. According to the preparation method, extraction of the exosome, loading of the prebiotics and an intestinal targeting modification process are optimized, so that the prepared intestinal targeting exosome loaded with the prebiotics can be used for remarkably improving the loading efficiency and intestinal targeting of the prebiotics and enhancing the stability of the exosome in gastrointestinal tracts; therefore, the delivery efficiency and the treatment effect of the prebiotics in the intestinal tract are improved.

The invention belongs to the field of functional substance research, and particularly relates to a nutritional composition for promoting absorption of fatty acid and mineral substances and application. The nutritional composition provided by the invention comprises necessary active ingredients shown as the following (I) and (II): (I) 1, 3-dioleic acid-2-palmitic acid triglyceride; (II) a milk fat globule membrane, and / or, 2 '-fucosyllactose; moreover, in the nutritional composition, the mass ratio of the necessary active component shown in (I) to the necessary active component shown in (II) is (5-10): (0.5-10). The combination of OPO and MFGM and / or 2 '-FL provided by the invention can be beneficial to promoting the absorption of the body to fatty acids and mineral substances, and especially can be beneficial to promoting the transmembrane transport capability of intestinal cells of the body to fatty acids and mineral substances, so that the absorption efficiency is improved.

The invention relates to the technical field of pharmaceutical preparations, and particularly provides colon-targeted pH-responsive nano-liposome and application thereof in improving oral bioavailability of saponin. Aiming at the problems of gastric acid damage, small intestine metabolism inactivation and low colon absorption efficiency during oral delivery of saponin, a three-layer core-shell structure design is adopted, wherein an inner core is composed of a saponin-phospholipid crystal compound; the middle layer is a chitosan-PEG bimolecular layer, and pH response release is realized in a colon environment with pH of 7.4; and the outer layer is modified with a folic acid-PE conjugate to enhance colon cell targeted recognition. The uniform nanoparticles with the particle size of 80 + / -5 nm are prepared by combining a gradient pH drug loading method with a microfluidic focusing technology. According to the preparation, the saponin bioavailability (AUC0-12 reaches 43.31 h. Microgram / mL) is remarkably improved, and the colon targeting property (the cell uptake rate is 52.9%) is enhanced. The innovativeness is that the gastrointestinal segmented protection and accurate colon release are synergistically realized through a multi-layer functional design, and the solubilization-permeation paradox of the traditional delivery technology is broken through.

The invention discloses a 3D (three-dimensional) visual bionic intestine-blood vessel-nerve interface organ simulation chip, which is characterized in that a micro-fluidic chip is used as a platform to construct an intestinal tract-blood vessel-brain system which simulates biological transformation of perfluorinated compounds in intestinal tracts under near physiological conditions and biologically transports the perfluorinated compounds through blood vessels; a neuronal cell culture chamber, a vascular endothelial cell culture chamber and an intestinal tract cell culture chamber are respectively arranged on the micro-fluidic chip, the culture chambers are communicated through micro-columns, and the vascular endothelial cell culture chamber serves as a barrier in the middle; perfluorinated compounds enter corresponding cell culture cavities for culture through reagent injection channels of different cell culture channels, generated metabolites are discharged through the channels, and multi-organ interaction and toxicity induced by the perfluorinated compounds are analyzed. The chip can simulate an intestinal barrier structure, a blood vessel and a neural network structure, can highly simulate biological transport and transformation of a perfluorinated compound between intestines and brains, and effectively reduces cost and time.

The invention provides a method for evaluating drug enterotoxicity in vitro through an intestinal organ model, and relates to the field of biological medicine. Comprising the following steps: preparing six different culture media, culturing the hESC by using the different culture media, inducing the hESC to respectively perform directional endoderm cell differentiation, posterior intestinal bulb differentiation, intestinal organ differentiation and intestinal organ maturation, and evaluating the drug enterotoxicity by using a prepared intestinal organ model. The intestinal organ model prepared by the invention is similar to human intestinal cells in composition, can accurately reflect the toxic effect of the medicine in the physiological environment in the human body, can be repeatedly obtained in quantity, and avoids clinical risks and ethical constraints; the sensitivity of drugs with different enterotoxicity can be reflected through cell viability, and the method is suitable for rapid evaluation of in-vitro high-flux drug enterotoxicity.

The invention discloses a liver-protecting beverage containing a composite plant exosome with optimized antioxidant capacity. The liver-protecting beverage comprises a plant exosome solution and a liver-protecting component, the plant exosome solution is a silybum marianum exosome solution and at least two of a Pingyin red rose exosome solution, a radix puerariae exosome solution, a turmeric exosome solution and a dandelion exosome solution; the liver protection component is at least one of silybum marianum micro-capsule powder, a radix puerariae extract, corn peptide, a dandelion extract and S-acetyl-L-glutathione. Hydroxyl and amido on small molecules are combined with lipid structures on the surfaces of multiple plant exosomes, small molecule active ingredients are combined through adsorption or reaction on the surfaces of the lipid structures of the exosomes, the oxidation resistance of the product can be improved, absorption of intestinal cells and probiotics is facilitated, cell metabolism is promoted, and then the fatigue feeling is reduced.

The invention provides an efficient muscle and weight gaining nanoemulsion and a preparation method thereof. The nanoemulsion is an O / W type nanoemulsion, a water phase coats creatine through maltodextrin to form a slow-release carrier, curcumin and inulin are coated in a double-layer mode in combination with phospholipid, intestinal cell absorption is enhanced through micelle membrane fusion, and the synergistic effect of curcumin anti-inflammation and inulin regulation flora is achieved; the oil phase adopts a chitosan-sodium alginate polymer to embed a BCAA / MCT compound, branched chain amino acid is released in an intestinal tract in a targeted manner to activate an mTOR pathway, MCT is slowly released to continuously supply energy, gastrointestinal discomfort caused by sudden rising of osmotic pressure is avoided, and the muscle and weight gaining efficiency is remarkably improved through a multi-component synergistic controlled release technology.

The invention discloses an in-vitro drug intestinal permeability determination method based on a high-flux barrier chip, and belongs to the technical field of microfluidics and organ chips. The high-flux barrier chip adopts a three-layer structural design of a pore plate layer, a porous membrane layer and a channel layer; the porous membrane not only realizes physical separation of the pore plate layer and the channel layer, but also undertakes the core function of interlayer substance exchange, and by matching with a gravity flow driving system provided by the swing table, the fluid dynamic characteristics in the chip are guaranteed. The hole pitch of the chip is strictly matched with the standard of a commercialized pore plate, and the chip can be directly compatible with various conventional analytical instruments and has good universality. The method is suitable for determining the intestinal permeability of the passive transport compound which can be absorbed by intestinal cells. Compared with the prior art, the human intestinal cells are adopted to construct the chip model, animal experiments do not need to be carried out, related ethical disputes are thoroughly avoided, the whole operation process is simple and easy to implement, and the detection time is greatly shortened.

The invention provides a lactobacillus fermentation extraction method of marine red algae polysaccharide and application of the marine red algae polysaccharide, and the method comprises the following steps: mixing marine red algae powder with water, and carrying out pasteurization at 60-70 DEG C for 20-40 minutes; adding lactobacillus plantarum into the sterilized marine red algae turbid liquid, and performing fermentation culture to obtain a marine red algae fermentation mixed liquid; heating and extracting the marine red algae fermentation mixed solution at 85-95 DEG C for 1.5-2.5 hours, centrifuging, collecting supernate, carrying out rotary evaporation and concentration to 1 / 5 of the original volume, then adding ethanol, standing overnight, discarding the supernate, adding distilled water into precipitate for redissolving, and deproteinizing by using a Sevag method to obtain a polysaccharide solution; and dialyzing the polysaccharide solution, and concentrating and freeze-drying the dialysate to obtain the marine red algae polysaccharide. According to the method, the low-molecular-weight modified polysaccharide is obtained through lactobacillus plantarum fermentation, extraction and purification, and the low-molecular-weight modified polysaccharide is high in sulfate group content, loose in structure, capable of remarkably inhibiting AGEs-induced intestinal cell oxidative stress and inflammatory reaction, capable of being used for preparing the glycosylation injury resisting medicine and good in industrialization prospect.

The application relates to application of iron-nitrogen doped graphene in preparation of anti-radioactive enteritis drugs and belongs to the technical field of biological medicines. ZIF-8 powder, 1,10-phenanthroline powder, iron (II) acetate powder and a grinding medium are placed in a closed container to perform mechanical ball milling treatment to obtain a mixed powder; under the protection of inert gas, the mixed powder is placed in a preheated tube furnace to perform pyrolysis treatment, and is cooled to room temperature to obtain iron-nitrogen doped graphene. The iron-nitrogen doped graphene has NADH oxidase-like activity, can directly catalyze the conversion of NADH into NAD + , can rapidly supplement NAD + depletion caused by radiation, can enhance PARP-dependent DNA damage repair, can reduce the generation of active oxygen (ROS), can reduce intestinal cell death and can protect the integrity of intestinal structure, and can realize efficient protection of radioactive enteritis. Moreover, the problem of low-efficiency biosynthesis of NAD + supplementation in existing radioactive intestinal injury protection technologies is solved.

The invention relates to the technical field of biology, in particular to recombinant lactic acid bacteria capable of improving the serum melatonin level and a construction method of the recombinant lactic acid bacteria. According to the recombinant lactic acid bacteria, melatonin synthetase AANAT and HIOMT genes are introduced through a genetic engineering technology and are stably expressed in genomes of the recombinant lactic acid bacteria, so that the recombinant lactic acid bacteria have the capability of secreting melatonin synthetase. The lactic acid bacteria are food-grade strains without resistance selection markers, have good biological safety and can enter intestinal tracts through oral administration. After oral administration, the recombinant lactic acid bacteria secrete melatonin synthetase in intestinal tracts, promote intestinal cells to synthesize melatonin, and improve the level of melatonin in serum through an intestinal-blood barrier. Serum detection shows that the recombinant lactic acid bacteria can significantly improve the concentration of serum melatonin. Compared with the prior art, the invention provides a safe and effective food-grade recombinant lactic acid bacteria preparation which is suitable for promoting the improvement of the endogenous melatonin level of a human body and has a wide application prospect.

The present application relates to a kind of high flux anaerobic intestinal chip system and the application in the function of evaluating probiotic bacteria to alleviate enteritis, high flux anaerobic intestinal chip system includes microfluidic chip, fluid perfusion device, oxygen monitoring chip and micro anaerobic box, fluid perfusion device can inject culture medium into microfluidic chip, oxygen monitoring chip is arranged in microfluidic chip upstream, microfluidic chip is arranged in micro anaerobic box, microfluidic chip is equipped with intestinal cell, high flux anaerobic intestinal chip can simulate intestinal environment, by different kinds of probiotic bacteria are added to intestinal chip, can be obtained by detecting culture product, or detecting intestinal cell after culture, to evaluate probiotic bacteria function;Further can combine high flux anaerobic intestinal chip with machine learning, utilize machine learning technology to analyze and process the multidimensional data characteristics (gene, protein, cell level) of enteritis model based on intestinal chip, to carry out global, overall comprehensive evaluation;Realize high flux, intelligent screening of better efficacy probiotic bacteria strain finally.

The invention discloses a medicine for relieving side effects of gastrointestinal tracts caused by nintedanib, a medicine for preventing or treating pulmonary fibrosis and application of stachyose, and belongs to the technical field of medicines. The medicine taking the stachyose as the active ingredient can effectively relieve gastrointestinal tract side effects caused by the nintedanib, for example, intestinal mucosa lesions caused by the nintedanib can be obviously relieved, for example, intestinal mucosa tissue structure abnormity, intestinal cell proliferation activity reduction, intestinal cell apoptosis increase, intestinal tissue inflammatory factor gene expression level up-regulation and the like.

The present invention discloses the application of the LI0004 protein target in the preparation of a drug for inhibiting the proliferation of Lawsonia infection cells, belonging to the field of biomedicine. IPEC-J2 cells were used to construct an in vitro Lawsonia infection model, and the mechanism of action of the LI0004 protein as a virulence factor in Lawsonia infection cells was studied. The relationship between the LI0004 protein in regulating the activation of the cell cycle signaling pathway and the toxic mechanism was elucidated. The results showed that the LI0004 protein directly bound to the Notch 1 protein, activated the Notch 1 signaling pathway, and then promoted the proliferation of IPEC-J2 cells. The present invention also constructed a mouse model infected with the LI0004 protein. The results showed that the LI0004 protein can produce pathological changes similar to Lawsonia infection in vivo, and the Notch 1 signaling pathway is an important mechanism for the intestinal cell proliferation caused by it.

This invention relates to the fields of biology and medicine, and discloses the application of suramin sodium in the prevention and / or treatment of radiation-induced intestinal injury. The inventors of this invention have discovered that suramin sodium treats radiation-induced intestinal injury caused by local ionizing radiation by: improving intestinal tissue damage, promoting intestinal crypt cell regeneration, inhibiting intestinal cell apoptosis, and reducing serum inflammatory factor levels; therefore, it can be considered a potential drug for treating radiation-induced intestinal injury.

The invention belongs to the technical field of animal nutrition and feed, discloses an application of a Fuzhuan tea extract in prevention of vomitoxin-caused piglet health injury, and particularly relates to an application of the Fuzhuan tea extract as a feed additive in prevention or improvement of piglet health injury. The feed additive can effectively reduce negative effects on weaned piglets caused by excessive vomitoxin in the feed, wherein the negative effects comprise growth performance reduction, diarrhea rate increase, intestinal morphology damage, intestinal cell proliferation inhibition, intestinal epithelial barrier injury, immune disorder, abnormal liver function and excessive oxidative stress and inflammatory response.

The invention discloses a tooth-benefiting xylitol tablet candy and a preparation method thereof. The tooth-benefiting xylitol tablet candy is prepared from the following raw materials: xylitol, modified sodium alginate, HPMCP-genipin, microcrystalline cellulose, colloidal silicon dioxide and magnesium stearate. After the HPMCP-genipin enters the enteric cavity, the shell is dissolved, the L-glutamine and the anhydrous dextrose maintain and stabilize the osmotic pressure of intestinal cells, the genipin of the inner core and the primary amine group on the molecular chain of the modified sodium alginate are covalently crosslinked to form a gel network to wrap the xylitol, and meanwhile, the gel network absorbs water to form a local isotonic environment. A gel network wrapping xylitol enters a large intestine and is dissolved under the action of coliform bacteria, and galactooligosaccharide selectively stimulates the growth of bacteria methanogens capable of utilizing hydrogen, so that the total volume and partial pressure of gas in the intestinal tract are reduced. The microcrystalline cellulose improves the viscous component of the modified sodium alginate, and the colloidal silicon dioxide reduces the shearing and extrusion on the HPMCP-genipin in the tabletting process.

The invention relates to an establishment method of a high-fat induced intestinal organ injury model and an injury intervention method for the model. The establishment method comprises the following steps: step A, preparation: preparing colon cells, PBMC cells and an organ-like culture chip for later use; step B, colon barrier construction: inoculating colon cells to the surface of a barrier membrane on the lower side of a culture chip by using an organoid culture chip to obtain a colon barrier model; step C, constructing a co-culture model, and inoculating PBMC cells to the basal side of the colon barrier on the basis of the colon barrier to obtain a human colon barrier-PBMC co-culture model; d, constructing a damage model, and performing damage treatment on the co-culture model by using a treating fluid to obtain the damage model; according to the invention, the barrier function organ-like chip is used, a human intestinal cell-PBMC co-culture model is formed by utilizing human intestinal organs and PBMC cells, and OXLDL / palmitic acid can play a role in an injured intestinal organ barrier model and form a high-fat induced intestinal organ injury model through the prepared injury treating fluid.

The application relates to the technical field of bioengineering, and discloses a method for constructing an inflammatory bowel disease model, which comprises the following steps: constructing an inflammatory intestinal barrier by using intestinal cells from an inflammatory bowel disease patient; adding a stimulating solution containing immune cells and / or immune cell secretions into the inflammatory intestinal barrier, and carrying out co-culture to obtain an inflammatory bowel disease model. The inflammatory intestinal barrier constructed by using intestinal cells from an inflammatory bowel disease patient and adding immune components can retain the gene and cell shape characteristic expression characteristics related to IBD diseases, so that the model is closer to the pathological condition in the patient body; the actual in-vivo pathological damage progress is simulated, the model bionics is improved, the damage of the unbalanced immune cells in the IBD pathology to the intestinal tract is reproduced, and the potential drug targets can be discovered and verified. The application further discloses an inflammatory bowel disease model and an application thereof.

This invention relates to the application of a traditional Chinese medicine composition in the preparation of a drug for treating intestinal injury. The traditional Chinese medicine composition comprises: 50-150 parts of ginseng, 60-180 parts of polygonatum, 40-90 parts of atractylodes, 30-58 parts of sophora flavescens, 60-180 parts of ophiopogon japonicus, 60-110 parts of rehmannia glutinosa, 40-90 parts of polygonum multiflorum, 60-180 parts of cornus officinalis, 40-90 parts of poria cocos, 35-58 parts of eupatorium fortunei, 35-58 parts of coptis chinensis, 35-90 parts of anemarrhena asphodeloides, 35-58 parts of epimedium, 40-110 parts of salvia miltiorrhiza, 60-180 parts of kudzu root, 80-140 parts of litchi seed, and 40-90 parts of lycium chinense root bark. The above-mentioned traditional Chinese medicine composition can inhibit the expression of TNF, AGE-RAGE, TLR and IL-17 and the infiltration of macrophages. It also helps to regulate the proliferation and differentiation of intestinal cells, restore beneficial bacteria in the intestine, and significantly improve the barrier function of the intestine, thereby effectively improving the damaged intestinal function.

The application provides a kind of tea polyphenol ester-RNA self-assembly and hybrid nanovesicle of probiotic outer membrane vesicle and preparation method thereof, which first adjusts the hydrophilicity and hydrophobicity of tea polyphenol EGC through molecular modification strategy, and the EGC palmitate obtained by modification can directly wrap RNA and self-assemble into tea polyphenol ester-RNA nanovesicle, which has superior encapsulation capacity. Further introduce OMVs as intestinal targeted delivery carrier and fuse with RNA-tea polyphenol nanovesicle to form a structure stable hybrid vesicle. The hybrid vesicle of the application ingeniously integrates the advantages of both sides: tea polyphenol ester-RNA nanovesicle ensures efficient encapsulation of RNA, and OMVs provide natural protection for its translocation through gastrointestinal tract, and promote intestinal cell uptake with its inherent bioadhesion. This strategy can effectively promote the encapsulation and stabilization of oral RNA delivery.

The invention discloses a nutritional composition for promoting absorption of amino acid and mineral substances and application, and belongs to the technical field of food. The nutritional composition provided by the invention comprises alpha-lactalbumin and breast milk oligosaccharide (especially 6 '-SL and / or 3-FL), and can help to promote absorption of amino acids and mineral substances by a body, especially help to promote transmembrane transport capability of intestinal cells of the body to amino acids and mineral substances, thereby improving absorption efficiency.

The application discloses a kind of for radiation intestinal injury treatment composition and its application, it is related to medical technical field.The composition includes the following components: aesculetin, fraxinol, fraxin and baicalein.The composition of the application can reduce intestinal villus injury by regulating the NF-κB pathway and Caspase-9 / 3 pathway of intestinal epithelial cells, reduce the infiltration of intestinal inflammatory cells, reduce intestinal cell apoptosis, to relieve intestinal injury, and then multi-component, multi-pathway jointly play the efficacy of treating radiation intestinal injury.The application solves the problem that there is no effective drug for treating radiation intestinal injury in the prior art.

The application discloses a composition for treating radiation enteropathy and application thereof, and belongs to the technical field of biological medicine, and solves the problem that there is no effective treatment drug for radiation enteropathy at present. The composition comprises puerarin, baicalein, berberine and glycyrrhetinic acid. The composition for treating radiation enteropathy can regulate Wnt / beta-catenin, NF-kappa B and Caspase-9 / 3 signal pathways and the like, promote the proliferation of intestinal cells after radiation damage, reduce the inflammation level of intestinal tissues after radiation, reduce the apoptosis of intestinal cells after radiation and the like, improve the radiation damage of intestinal epithelial cells, play an anti-radiation enteropathy role, and has a remarkable effect on treating radiation enteropathy.

This invention discloses a strain of *Lactobacillus paracasei* capable of converting cyanocobalamin and promoting its absorption, thereby enhancing brain nerve repair and improving cognition, and its postbiotic, belonging to the fields of microbial technology and pharmaceutical technology. The *Lactobacillus paracasei* CCFM1527 and its prepared postbiotic provided by this invention promote the growth of vitamin B1 in the host. 12 The neuroprotective effects of upregulating the expression of absorption receptors and converting CN-CbI to methylcobalamin include: (1) promoting the upregulation of Amnionless expression in intestinal cells (Caco-2); (2) converting CN-CbI to methylcobalamin; and (3) promoting the upregulation of Amnionless expression in mouse ileum. AMN Upregulation of gene expression (4) significantly increased the levels of vitamin B in mice. 12 (5) Significantly promotes the expression of MBP and PSD-95 proteins in mice; (6) Improves the decline in cognitive ability in mice, and has great application prospects.

The present disclosure relates to a novel (Lactococcus lactis subsp. cremoris HEM 20103 strain useful for promoting intestinal absorption of calcium, and uses thereof. The novel Lactococcus lactis HEM 20103 strain of the present invention was confirmed to exhibit acid resistance, bile resistance, and intestinal cell adhesion ability and to have an excellent effect of promoting intestinal absorption of calcium. In addition, by increasing bone mineral content, the strain was confirmed to increase calcium metabolism, bone density, bone volume per unit volume, and cortical bone thickness and to significantly improve trabecular bone thickness, trabecular number per unit area, and trabecular spacing in osteoporosis model mice. Therefore, the strain of the present invention is expected to be widely used as a health functional food composition, a feed composition, a feed additive, and the like for promoting intestinal absorption of calcium and to have an excellent effect of preventing or treating intestinal absorption-related diseases of calcium such as osteoporosis or osteopenia.

The application provides a scophthalmus maximus intestinal tissue cell line, which has a preservation number of CCTCC NO: C2022231. The scophthalmus maximus intestinal tissue cell line provided by the application can be continuously subcultured, has been subcultured to 60 generations and remains stable, can provide a large number of stable scophthalmus maximus intestinal cells, and is used for researches on immune function genes and epithelial adhesion function genes. The provided cell line has excellent traits, is a fibroblast, the cells are fusiform or irregular triangular, the cell bodies extend several irregular protrusions outward, and the cells are closely arranged. The cell line can be subcultured once every 3-4 days, has high resuscitation efficiency, and can be used for plasmid transfection, siRNA interference and various molecular biology experiments.

This invention relates to the fields of biomedicine and food nutrition, specifically to the application of purple sweet potato anthocyanins in the preparation of products for preventing or improving intestinal aging. This invention demonstrates that purple sweet potato anthocyanins can significantly improve the degenerative changes and barrier fragility of the colon caused by natural aging, restore colonic crypt morphology and epithelial ultrastructure, and upregulate the expression of tight junction proteins and mucus barrier-related indicators. Furthermore, purple sweet potato anthocyanins can effectively enhance crypt proliferation activity and promote the differentiation of intestinal stem cells into secretory lineages (such as goblet cells and enteroendocrine cells). Its mechanism of action does not rely on direct anti-inflammatory pathways, but rather on remodeling intestinal flora metabolism (such as increasing short-chain fatty acids) and activating intestinal cell autophagy via the PI3K-Akt signaling pathway, thereby supporting the continuous renewal and maintenance of the intestinal barrier. This invention provides a new approach for the development and application of food-derived polyphenols targeting the gut to promote healthy aging.

The invention belongs to the technical field of microorganisms, and discloses a strain of lactobacillus plantarum which has the effects of resisting oxidation, detoxifying and relieving ulcerative colitis, and the preservation number of the lactobacillus plantarum is GDMCC (China General Microbiological Culture Collection Center) NO.66774. The strain has a free radical scavenging capability, can improve the antioxidant enzyme activity of intestinal cells, and can degrade endotoxin; meanwhile, the strain can regulate the immune balance of the organism, remodel the homeostasis of intestinal flora, promote the generation of acetic acid, propionic acid and butyric acid, effectively repair the damaged intestinal mucosal barrier, and improve the ulcerative colitis symptom from multiple dimensions in a synergistic manner. Therefore, the strain can be used for preparing functional foods, nutritional supplements and microecologics with the effects of resisting oxidation, expelling toxins, protecting intestines and preventing and treating ulcerative colitis.