157 results about "Enterocyte" patented technology

The invention discloses a nutritional adsorbent used for relieving pigling mycotoxin poisoning. The nutritional adsorbent is composed of, by mass, 4 to 5% of vitamin E, 10.08 to 12% of sodium butyrate, 11 to 12% of astragalus polysaccharide, 20 to 30% of glucomannan, and 42 to 53% of HSCAS. The intestinal nutritional agent is capable of providing enterocytes with nutrients, so that under mycotoxin stressed conditions, the nutritional adsorbent is beneficial for protection of structural and functional completeness via intestinal mucosa rapid repairing, is capable of promoting transportation and absorption of glucose and amino acid, and reducing feed conversion ratio obviously; the nutritional adsorbent is capable of increasing pigling intestinal antioxidant capacity, and relieving intestinal oxidative damage caused by mycotoxin; the nutritional adsorbent is used for maintaining intestinal health via adjusting pigling intestinal flora; and excellent improvement effects on poisoning caused by a plurality of mycotoxins are achieved via nutrition regulation.

The present invention is directed to a cargo-loaded cholesteryl ester nanoparticle with a hollow compartment (“cholestosome”) consisting essentially of at least one non-ionic cholesteryl ester and one or more encapsulated active molecules which cannot appreciably pass through an enterocyte membrane in the absence of said molecule being loaded into said cholestosome, the cholestosome having a neutral surface and having the ability to pass into enterocytes in the manner of orally absorbed nutrient lipids using cell pathways to reach the golgi apparatus. Pursuant to the present invention, the novel cargo loaded cholestosomes according to the present invention are capable of depositing active molecules within cells of a patient or subject and effecting therapy or diagnosis of the patient or subject.

The present invention provides an intestinal tract multi-modal movement integrated three-dimensional cavity intestinal organ-on-a-chip and a method. The intestinal tract multi-modal movement integrated three-dimensional cavity intestinal organ-on-a-chip comprises a cavity porous thin film for culturing and differentiating intestinal epithelial cells, a supporting thin film is arranged on outside of the cavity porous thin film, the supporting thin film is connected with gas channels, the supporting thin film is driven to move through air inlet / exhaust of the gas channels so as to deform the cavity porous thin film to simulate actions of stressor contraction; a cavity with an accommodating area is arranged outside the cavity porous thin film, can provide a culture solution perfusion area forthe cavity porous thin film and forms a micro-mechanical environment with axial and circumferential shear stress; and a periphery of the cavity porous thin film is embedded with a plurality of closedmagnetic tapes, the closed magnetic tapes can generate magnetic fields along with electrification of a solenoid movably arranged outside the cavity, so that a radial contraction action is generated at corresponding positions of the cavity porous thin film.

The invention discloses a method for separating and purifying leech polypeptide. The method comprises the following steps: adding a leech extract into a system capable of simulating human body enterocyte absorption for culturing; performing chromatographic separation on absorbed substances. By adopting the method, the problem of difficulty in separating and purifying the leech polypeptide is solved from different aspects. The leech extract is purified by Caco-2 cells firstly to simulate the digestive absorption process of leech serving as traditional Chinese medicine in a human body, and a large quantity of polypeptides which cannot be absorbed by human body enterocytes are separated out by simulation of the absorption functions of the enterocytes, so that a separating-purifying process is simplified. Then, polypeptides which can be absorbed by the human body can be separated and purified through anion exchange chromatography and gel filtration chromatography. The polypeptides obtained by separation and purification according to the method have the effect of restraining migration of vascular smooth muscle cells.