49 results about "Acid tolerance" patented technology

This invention discloses a strain of Bifidobacterium animalis subspecies TRT-2A with allergy-relieving effects and its extracellular vesicle applications. The strain TRT-2A is deposited at the China Center for Type Culture Collection (CCTCC) on November 21, 2025, with accession number CCTCC NO:M 20252645. This strain is highly acid-resistant and exhibits good tolerance to artificial gastric and intestinal fluids. It effectively inhibits bacteria, particularly Streptococcus pyogenes, Streptococcus agalactiae, and / or Escherichia coli. Experimental results show that this strain can alleviate hormone-dependent dermatitis and effectively improve dermatitis symptoms such as erythema and desquamation. Furthermore, extracellular vesicles prepared using TRT-2A can effectively improve skin elasticity and reduce wrinkles. Therefore, the Bifidobacterium animalis subspecies TRT-2A of this invention has multiple functions and is worthy of further promotion and application.

This invention belongs to the field of wastewater treatment, specifically relating to a composition, treatment agent, and application for treating oily wastewater and oily sludge. The composition comprises component A and component B, with a mass ratio of (40-100):(5-8). Component A consists of cyclodextrin, sodium alginate, and glycerol; component B is acrylamide. This invention uses cyclodextrin, sodium alginate, glycerol, and acrylamide as main components, containing functional groups such as polycarboxyl, polyhydroxy, carbonyl, and amino groups. It utilizes hydrogen bonding, covalent bonding, and electrostatic interactions between these functional groups to achieve rapid treatment of wastewater or sludge. Experiments show that this composition has good acid resistance and adsorption capacity, achieving the removal of oil and suspended solids from acidic oily wastewater and sludge without pre-oxidation or pH adjustment, effectively reducing treatment costs and simplifying the treatment process.

This invention discloses a strain of *Pediococcus pentosaceus*, FREE-U AP01, and its applications. The strain, FREE-U AP01, has the accession number CGMCC No. 37267. This invention screened and obtained a strain of *Pediococcus pentosaceus*, FREE-U AP01, which, through its excellent temperature adaptability, high survival rate in acid and bile salt tolerance, rapid acid production capacity, and significant antibacterial activity, solves the problems of low survival rate and unstable antibacterial effect of existing probiotics, providing a powerful and high-quality strain resource for microecological preparations and animal feed additives.

This invention relates to the field of biomedical technology, specifically to a subspecies of Bifidobacterium animalis B189 and its applications. The classification name of this strain is... Bifidobacterium animalum subsp . animal The accession number is CGMCC No. 37052. Bifidobacterium animalis B189 not only exhibits significant antibacterial effects against Helicobacter pylori, but also possesses extremely strong acid and bile resistance, making it valuable for the development of microbial agents to inhibit Helicobacter pylori.

The application discloses a kind of biological hybrid micro machine population oral delivery system of imitating Helicobacter pylori survival mechanism, belong to oral stomach medicine delivery technical field.The system is by active power module (flagellum driven chlamydomonas reinhardtii), environmental regulation module (acid urease modified on algal surface) and multifunctional load module (macrophage membrane wrapped drug-loaded nanoparticles) synergistic assembly through polydopamine (PDA) spatial conformation control layer is formed.Active power module utilizes self-movement ability to penetrate mucus barrier and extend intracavity retention time to 12 hours or more;Environmental regulation module produces local alkalization effect by catalyzing urea hydrolysis, provides chemical shield for system in extremely acid environment of pH 1.0-3.0, protects active power module from gastric acid inactivation;Multifunctional load module is accurately delivered to gastric epithelial cells by inflammation targeting effect, such as CRISPR / Cas9 biological macromolecule, and realizes up to 31.8% in-vivo gene editing efficiency.The application provides a non-invasive, modular active delivery platform for the precise treatment of gastric diseases such as gastritis through the synergistic effect of physical penetration, metabolic reprogramming and gene intervention.

This invention discloses a highly acid-resistant Bacillus belye strain LYBV022-Ac R This invention relates to a method for screening and acclimating bacteria under progressive acid stress, and its application, belonging to the technical field of industrial microbial breeding and probiotic application. The disclosed high-acid-resistant Bacillus belyeis LYBV022-Ac... R By designing a pH gradient decreasing (6.5→3.0) acclimatization strategy and combining it with microplate rapid screening technology, a breakthrough improvement in acid resistance was achieved. After exposure to a simulated gastric acid environment (pH 3.0, containing pepsin) for 4 hours, the survival rate reached 48.52%, a 970-fold increase compared to the original strain (0.05%), while maintaining excellent growth performance and genetic stability. This invention also provides the application of this strain in the preparation of oral probiotic preparations for livestock and poultry, solving the common technical problem of insufficient potency in oral Bacillus preparations due to gastric acid damage.

This invention belongs to the field of microbial technology, specifically relating to a *Clostridium butyricum* strain, its culture conditions, and applications. This invention provides a novel *Clostridium butyricum* strain with strong resistance to high temperatures, acids, alkalis, and salts, exhibiting high biocompatibility and no significant toxic side effects or hemolytic activity. Therefore, the *Clostridium butyricum* H2-2 is a potential probiotic strain that can be used as feed or feed additive in pig, poultry, and aquatic feeds for animal husbandry, strengthening the intestinal barrier and enhancing animal immunity; it also holds promise for use as an additive in human food, pharmaceuticals, and health products.

This invention discloses *Paenibacillus alvei* CHWJ-P3 for dissolving phosphorus and potassium in acidic soils and its applications. *Paenibacillus alvei* CHWJ-P3 is classified as *Paenibacillus alvei* CHWJ-P3 and was deposited at the China Center for Type Culture Collection (CCTCC) on December 8, 2025, with accession number CCTCC NO: M 20252813. The *Paenibacillus alvei* CHWJ-P3 of this invention exhibits a significant ability to dissolve phosphorus and potassium in the environment, along with high acid resistance and efficient pH regulation. It can be used for the release of insoluble phosphorus and potassium in soil, i.e., the bioremediation of phosphorus and potassium dissolution in acidic soils; and for the bioremediation of acidic soil pH regulation. It features high remediation efficiency, stable remediation effects, and no secondary pollution, demonstrating promising application prospects.

This invention provides an acid- and alkali-producing bacterial combination, a compound bacterial agent, and their applications, belonging to the field of microbial technology. This invention provides a bacterial combination including Bacillus subtilis with accession number CGMCC No. 29201. Bacillus sp S1 and Arthrobacterium with accession number CGMCC No. 29202 ( Arthrobacter sp S2. In this invention, both Bacillus S1 and Arthrobacter S2 are acid- and alkali-producing strains with strong environmental adaptability. Through synergistic effects via multiple pathways, including ammonia production, bicarbonate secretion, and activation of soil enzymes, the bacterial combination can significantly increase soil pH, reduce exchangeable aluminum and hydrolyzable acids, and simultaneously increase soil bicarbonate content, thus rapidly and comprehensively improving soil acidity from its root cause. This bacterial combination is of great significance for achieving green, efficient, and sustainable remediation of acidified soils.

This invention discloses a microorganism, a microbial agent, and their applications. The microorganism is *Zygosaccharomyces rouxii*, which was deposited on May 27, 2024, at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 30794. The novel *Zygosaccharomyces rouxii* strain obtained by this invention exhibits rapid growth, strong salt and acid tolerance, and a strong ability to metabolize nitrates and nitrites. Food or feed production methods developed using this novel strain and microbial agent demonstrate high salt tolerance, high fermentation efficiency, and a short production cycle, effectively reducing nitrate and nitrite content during fermentation. Fermented foods made using this novel strain are rich in yeast, low in nitrates and nitrites, and are nutritionally healthy with a unique flavor.

The present application relates to the technical field of microorganism, in particular to a leuconostoc lactis with good probiotic characteristics and application thereof in preparation of drugs for preventing and treating inflammatory bowel disease, and the leuconostoc lactis (Leuconostoc lactis) LL271 provided by the present application is preserved in China General Microbiological Culture Collection Center, and the preservation number is CGMCC No. 25974.The strain has good acid tolerance, bile salt tolerance, self-aggregation ability, hydrophobicity and other probiotic characteristics such as the ability to inhibit common human pathogenic microorganisms; meanwhile, the strain can improve intestinal inflammation, has prevention and treatment effects on inflammatory bowel disease, has high biological safety, can be used as probiotic preparation for relieving inflammatory bowel disease, improving intestinal mucosal injury and colon inflammation, provides a new strategy for preventing and treating inflammatory bowel disease, and has a potential clinical application prospect.

The application discloses a glucose oxidase mutant with simultaneously improved thermal stability and acid resistance and antibacterial application thereof, and belongs to the field of genetic engineering and protein engineering. The application takes glucose oxidase AiGODL as a mother to obtain a mutant AiGODL_E148K / F283Y by respectively mutating Glu148 and Phe283 into Lys148 and Tyr283. It is found through experiments that, compared with wild-type glucose oxidase AiGODL, the mutant AiGODL_E148K / F283Y has improved heat resistance and acid resistance, and has the same level of specific activity and catalytic efficiency as AiGODL and the same antibacterial performance as ampicillin. The mutant AiGODL_E148K / F283Y provided by the application has great application prospect in antibacterial and antibiotic substitution.

This invention discloses a lactic acid bacterium and its application, belonging to the field of microbial fermentation technology. The lactic acid bacterium is *Lactobacillus plantarum* (Lactobacillus plantarum). Lactiplantibacillus plantarum FYcg1, with preservation number CGMCC No. 33190, is a strain that produces high levels of acid, is resistant to acid, salt, and bile salts, and exhibits a nitrite degradation rate exceeding 99%. It also shows significant inhibitory effects on Escherichia coli and Staphylococcus aureus. This invention optimizes the preparation method of a direct-inoculation starter culture, using skim milk powder, sucrose, and trehalose as a composite protective agent. The starter culture is prepared through specific centrifugation, pre-freezing, and vacuum freeze-drying, resulting in a high survival rate of live bacteria and good storage stability. When used for fruit and vegetable fermentation, it can rapidly lower pH, increase total acid content, and optimize flavor and metabolite composition, effectively solving problems such as long natural fermentation cycles, uneven quality, and poor safety. It is suitable for the industrialized and standardized production of pickled bamboo shoots.

The application discloses a dog-derived Enterococcus faecalis ZJUIDS-D016 with anti-aging effect and application, the dog-derived Enterococcus faecalis ZJUIDS-D016 is classified and named as (Enterococcus faecalis) Enterococcus faecalis ), and the preservation number is CGMCC 27038.The strain of the application is isolated from the intestinal tract of a healthy dog, has acid resistance, bile salt resistance and broad-spectrum antibacterial activity, and is sensitive to chloramphenicol, erythromycin and penicillin.The strain of the application has strong host adaptability, can colonize in the intestinal tract of a dog and secrete organic acids and bacteriocins, significantly inhibits the proliferation of common pathogenic bacteria such as dog pathogenic escherichia coli, effectively improves the intestinal health of pets and delays aging, and is suitable for preparing pet probiotic products.

This invention discloses a powdered *Miller's yeast* strain and its application in food fermentation, belonging to the field of food biotechnology. The invention provides a powdered *Miller's yeast* strain GZR-YJ-40, preservation number: GDMCC No: 64935. The powdered *Miller's yeast* GZR-YJ-40 has the following characteristics: rapid growth rate; tolerance to the gastrointestinal environment and excellent probiotic properties; sensitivity to common fungal antibiotics, non-hemolytic, and good safety; effective tolerance to high salt, acid, and ethanol; antioxidant properties; and the ability to produce high levels of flavor compounds during fermentation. Furthermore, the powdered *Miller's yeast* GZR-YJ-40 has biogenic amine degradation capabilities; therefore, as a starter culture, this strain can ensure the safety and flavor quality of fermented foods, shorten the fermentation cycle, and significantly improve the nutritional value of fermented foods.

The application provides a cat-derived fermented lactobacillus muciadegus ZY001, application and product thereof, and relates to the technical field of biology. The fermented lactobacillus muciadegus ZY001 provided by the application is derived from a cat, and the inventor has found that the strain has good high-temperature resistance, acid resistance and bile salt resistance, can effectively inhibit the growth and proliferation of various diarrhea pathogenic bacteria, can be used for preventing or treating diarrhea, has antioxidant capacity, can relieve oxidative stress, is beneficial to animal anti-aging, and prolongs the life of a pet; has very strong self-aggregation and moderate hydrophobicity, and is beneficial to the strain in intestinal colonization and long-term exertion of the probiotic function.

The invention discloses a microorganism, a microbial agent, food and a preparation method of the food, and relates to the field of microorganisms. The microorganism is hansenula polymorpha, and the preservation number of the microorganism is CGMCC (China General Microbiological Culture Collection Center) No.30796. The hansenula uvarum new strain obtained through screening is high in growth speed and high in acid resistance and salt resistance, especially can normally grow in a 3 mM nitrite environment, can inhibit growth of escherichia coli and staphylococcus aureus and is high in nitrate metabolism capacity; the fermented food prepared from the new strain is low in salt content, nutritional, healthy and unique in flavor; a food fermentation process developed by utilizing the new strain is high in fermentation efficiency, short in production period and high in application value.

The present application relates to a lactobacillus johnsonii producing a novel class III bacteriocin, an engineered bacterium and application thereof, and belongs to the technical field of microorganisms. Lactobacillus johnsonii The JLJ071 strain of the lactobacillus johnsonii is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC No.34541. The bacteriocin amino acid sequence is shown in Seq ID No.1. The expressed novel class III bacteriocin has good bactericidal activity on listeria monocytogenes and salmonella pullorum, and has good characteristics such as acid resistance, high temperature resistance and protease resistance, and has a good application prospect in feed additives.

This invention relates to a Bacillus subtilis ( Bacillus subtle Y32, with accession number CGMCC No. 34819, is a strain that produces heat-resistant and highly stable xylanase, as well as various hydrolases such as β-mannanase and protease. It also produces extracellular polysaccharides, inhibits Botrytis cinerea, and is acid-resistant, resistant to porcine bile salts, and resistant to 10% NaCl. The optimal temperature for the xylanase produced by this strain is 55℃, and the optimal pH is 6.0. Under these conditions, the half-life is 12.5 h, and the relative enzyme activity remains above 80% after 3 h of incubation, demonstrating good thermal stability. This strain can also increase the yield of cucumbers and Pinellia ternata, and increase the egg production rate of 70-week-old Hy-Line Brown hens from 70.4% to 90.1%. This strain has promising applications in agriculture, food processing, and cosmetics production.

This invention discloses a compound microbial agent for targeted improvement of acidified soil in the red soil region of Guangxi and its application, belonging to the field of agricultural microbial technology. Addressing the limited effectiveness of traditional improvement methods, this invention provides a microbial agent composition made by compounding acid-tolerant alkali-producing strains, nitrogen-fixing strains, and phosphorus-solubilizing strains in a specific ratio, combined with a synergistic carrier to enhance the colonization rate of the strains. Through the synergistic effect of the strains, this agent can stably colonize and continuously produce alkali in an acidic environment, simultaneously improving the availability of soil nutrients. This invention also discloses an application method suitable for Guangxi fields, which is simple and highly adaptable. This invention can raise the pH of acidified soil by 0.8 to 1.2 units within 30 days, significantly improve soil physicochemical properties, promote crop growth, and achieve a yield increase of ≥12% per mu (approximately 0.067 hectares). It is environmentally friendly, long-lasting, and stable, making it suitable for the ecological restoration and large-scale production of acidified farmland in Guangxi.

The application discloses a bacterial agent for preventing senile dementia and belongs to the technical field of microorganisms. The Weizmannia coagulans A921 is screened, has relatively wide antibacterial property on the basis of excellent acid tolerance and bile salt tolerance, has a good relieving effect on Alzheimer's disease, and can improve behavioral characteristics and biochemical indexes. Further, the Weizmannia coagulans A921 is compounded with Weizmannia coagulans S511, a Weizmannia coagulans compound bacterial agent with a better effect than a single strain is obtained, and the Weizmannia coagulans compound bacterial agent has a wide application prospect in the preparation of anti-Alzheimer's disease products.

This invention discloses a xylanase Pexyn116 derived from *Pleurotus eryngii*, its truncated form for removing CBM1, and its applications, belonging to the field of microbiology and biotechnology. This invention provides xylanase Pexyn116 and its truncated form Pexyn116ΔCBM1. The xylanase Pexyn116 is acid-resistant, with an optimal pH of 4.5, exhibiting good stability within the pH range of 4.5 to 7.5; its optimal temperature is 50℃, and it retains more than 50% of its enzyme activity after treatment at 50℃ for 1 hour. The truncated form Pexyn116ΔCBM1 is also acid-resistant, with an optimal pH of 4.5, exhibiting good stability within the pH range of 4.5 to 7.5; its optimal temperature is 50℃, and it retains more than 70% of its enzyme activity after treatment at 50℃ for 1 hour. The xylanase Pexyn116 and its truncated form Pexyn116ΔCBM1 provided by this invention have excellent enzymatic hydrolysis effects on beech xylan and corn cob xylan, and have high application prospects and promotion value.

本发明提供一种植物乳杆菌（Lactiplantibacillus plantarum）DC76及其应用，所述菌株分离自贵州传统发酵食品豆豉，已于 2025 年 11 月 26 日保藏于广东省微生物菌种保藏中心，保藏号为GDMCC No.67360。该菌株具备优异的耐酸、耐胆盐特性，对大肠杆菌、沙门氏菌、巴氏杆菌等多种动物致病菌具有显著抑制作用；可调节肠道微生态平衡，上调肠道紧密连接蛋白 ZO‑1、Occludin 表达，增强肠道屏障完整性、降低肠道通透性，修复受损肠道屏障，同时调节肠道免疫微环境、减轻炎症反应。本发明所述菌株可用于制备动物饲料添加剂、微生态制剂及生物发酵剂，在畜禽健康养殖及替抗领域具有良好应用前景。

This invention discloses a strain of *Bifidobacterium longum* SRU-1, possessing both bile salt hydrolase activity and α-glucosidase inhibitory activity, with accession number CGMCC No. 36397. This strain was isolated from infant feces and identified as *Bifidobacterium longum* through morphological and molecular biological analysis. It exhibits excellent acid and bile salt tolerance, is non-hemolytic, and demonstrates superior self-aggregation and hydrophobicity. It can scavenge DPPH free radicals and possesses potential for lowering cholesterol and assisting in blood sugar control. This invention also provides its culture method; optimizing the culture medium can significantly increase the bacterial density, with the viable cell count reaching 1.34 × 10⁻⁶ when 20% prickly pear juice is added. 11 The concentration of CFU / mL is 5.8 times that of TPY medium. This strain can be used to ferment yogurt, producing a product with an acidity of 100°T-110°T and pH 4.4, excellent water retention, and combining the functions of probiotics with the nutritional advantages of prickly pear. It fills the technological gap in prickly pear multifunctional yogurt and is suitable for industrial food production.

The present application belongs to the technical field of industrial microorganisms, and particularly relates to an acid-tolerant high-ester-aroma-producing Zygoascus sieboldii and application thereof. Cyberlindnera jadinii The Zygoascus sieboldii provided by the present application is classified and named as GJLJ20, has been preserved in the China Center for Type Culture Collection (CCTCC) on November 19, 2025, and has a preservation number of CCTCC NO: M 20252589. The Zygoascus sieboldii of the present application has excellent acid tolerance and high flavor synthesis capacity. In a high-acid stress environment with a lactic acid volume concentration of up to 4% and a pH as low as 3.0, the Zygoascus sieboldii can still grow well (OD600 reaches 2.27±0.4), and can efficiently synthesize key flavor esters of wine, including phenethyl acetate, isoamyl acetate and ethyl acetate, thereby significantly enhancing the richness and level of wine aroma.

This invention discloses the application of L-tryptophan in the preparation of anti-caries oral care products, belonging to the field of biomedical technology. Addressing the safety issues, oral microecological disruption, and susceptibility to drug resistance associated with existing anti-caries agents (such as fluoride and chlorhexidine), this invention proposes using L-tryptophan as the effective active ingredient in anti-caries agents. Utilizing the specific effects of L-tryptophan on *Streptococcus mutans*, it achieves the effects of inhibiting tooth decay, maintaining oral microecological balance, and reducing the risk of bacterial drug resistance. This specific effect, without affecting the survival of *Streptococcus mutans*, simultaneously inhibits the synthesis of water-insoluble extracellular polysaccharides, inhibits acid production and acid tolerance, and inhibits oxygen tolerance and interspecies competition. It is characterized by its clear efficacy and high safety.

The application discloses a kind of for screening of key regulatory factor of acid resistance of corynebacterium glutamicum resolving method and application, belong to the cross field of microbial genetic regulation, bioinformation calculation and industrial fermentation engineering.The resolving method for screening of key regulatory factor of acid resistance of corynebacterium glutamicum provided, including: the strain expression data of corynebacterium glutamicum under different fermentation acidity conditions are arranged;Initial candidate relationship framework model is constructed;Directional flow characteristic kernel function under the condition of multi-condition expression mode is constructed, and is combined with initial candidate relationship framework model, to infer the directionality influence intensity;The stability of the obtained directionality relationship is verified;Possible direction determination and link rationality check are carried out, and then the acid resistance regulation link and key gene are output.The present application can provide a kind of high reliability calculation means for the acid resistance improvement and precise positioning of key regulatory factor of industrial fermentation strain.

The present application belongs to the field of microbial technology, and particularly relates to a lactic acid bacteria strain and application thereof. The strain is Ligilactobacillus salivarius SunLab-GHL02 (L. salivarius GHL02 for short), which is isolated from the lungs of high-performance Arbor Acres broilers, has been preserved in China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC NO: M 20251726. The classification status and functional characteristics of the strain are determined through morphological, physiological and biochemical, 16S rRNA and whole genome identifications. The strain has good acid tolerance, bile salt tolerance and acid production capacity, and can significantly inhibit pathogenic bacteria such as Escherichia coli, pathogenic E. coli, Salmonella, Staphylococcus aureus and Pseudomonas aeruginosa. Animal experiments show that atomization administration of the strain can improve the growth performance and feed conversion rate of poultry, enhance the stress resistance and promote health. The strain and its application can be widely used in the development of feed additives, probiotic preparations and animal health products, and are suitable for improving the health and production performance of poultry under intensive breeding conditions, and have good application prospects.

This invention relates to the field of biomedical technology, disclosing a targeted probiotic nanocapsule, its preparation method, and its application. The method involves using a photosensitizing agent ICG and polysaccharides to covalently couple probiotics to prepare probiotic nanocapsules. The core material of the microcapsule is primarily probiotics, and the microcapsule wall material is carboxymethyl dextran and the photosensitizing agent ICG. These microcapsules are easy to prepare, exhibit good sustained-release and controlled-release properties, and the materials are non-toxic, biocompatible, and biodegradable. This invention improves the wall material of the probiotic microcapsules and designs the preparation method and overall process accordingly. By utilizing polysaccharides and photosensitizing agents to form the capsule wall, the resulting nano-probiotics are acid-resistant, bile salt-resistant, digestive enzyme-resistant, and heat-resistant, resulting in improved probiotic stability and enhanced intestinal colonization.

The present application relates to the technical field of microorganism, and particularly relates to a Pediococcus pentosaceus QHEK025, a composition containing the same and purposes. The Pediococcus pentosaceus is Pediococcus pentosaceus QHEK025, the preservation number is CCTCC NO: M 20252985, and the preservation is carried out in China Center for Type Culture Collection. The composition contains at least one of lactic acid dodecylbenzene and a culture of the strain. The composition is used for preparing a composition for feed fermentation. Advantages of the present application are as follows: (1) can efficiently degrade mycotoxins; (2) has probiotic properties, strong growth performance, strong acid tolerance, strong bile salt tolerance and strong osmotic pressure tolerance; (3) has good hydrophobicity and self-aggregation performance; (4) high safety.