231 results about "Acid tolerance" patented technology

The invention belongs to the technical field of microorganisms, and particularly relates to bacillus cereus as well as a culture and application thereof. The preservation number of the bacillus cereus AK-LY1 is CCTCC (China Center For Type Culture Collection) NO: M 2025756. The bacillus cereus AK-LY1 disclosed by the invention is excellent in trypsin resistance, good in cholate resistance, acid resistance and high temperature resistance, good in inhibition performance on antibiotics such as strong penicillin and chloramphenicol, and relatively good in adhesion capacity in intestinal tracts, and provides a relatively good microecological preparation reference basis for expanding a bacillus cereus resource library and applying the bacillus cereus.

The invention provides low-temperature alginate lyase and application thereof, namely alginate lyase with low-temperature catalytic activity, which is separated from marine-derived cold monad bacteria, and the amino acid sequence of the alginate lyase is SEQ ID NO: 1; the amino acid sequence of the alginate lyase after the signal peptide is removed is SEQ ID NO: 3. The algin lyase Alg3889 provided by the invention has the optimal reaction temperature of about 10-16 DEG C, has good temperature stability, and still keeps more than 70% of enzyme activity after being incubated at 0-70 DEG C for 1 hour; the optimum pH value of the strain is 8.0, after the strain is incubated in a buffer system with the pH value of 3.0-10.6 for 12 h, about 90% of the highest enzyme activity can be kept, and the strain has high acid resistance and alkali resistance. The degradation product of the alginate oligosaccharide is alginate oligosaccharide with the polymerization degree of 2-6, and the alginate oligosaccharide has high application value.

The invention discloses an acid mine pollution treatment method based on microbial regulation and control, and belongs to the technical field of environmental protection engineering, and the acid mine pollution treatment method comprises the following steps: S1, screening and domesticating acid-resistant and heavy metal-resistant functional microbial communities; s2, constructing a sectional bioreactor, and filling the sectional bioreactor with a corresponding composite biological carrier; s3, inoculating the functional microbial community obtained by domestication in the step S1 into the sectional reactor constructed in the step S2, and carrying out biofilm culturing in simulated AMD; and S4, introducing AMD to be treated into the reaction system in the step S3, and regulating and controlling microbial activity through pH gradient regulation and dynamic supply of electron donors to realize heavy metal precipitation and water quality remediation. According to the method, closed-loop treatment of sulfate reduction, heavy metal precipitation and acid neutralization is achieved through the synergistic effect of microbial regulation and control and the functionalized biological carrier, and the problems of efficiency, cost and secondary pollution of traditional AMD treatment are solved.

The invention belongs to the technical field of microbiological detection, and discloses a PCR primer combination for detecting mixed microorganisms in a food fermentation process and a detection method thereof. The primer combination comprises a primer pair used for detecting pediococcus pentosaceus, a primer pair used for detecting plant lactobacillus, a primer pair used for detecting lactobacillus mucus fermentation, a primer pair used for detecting bacillus amyloliquefaciens and a primer pair used for detecting lactobacillus acidophilus. The primer pair is at least one of a primer pair for detecting lactobacillus helveticus and a primer pair for detecting acetobacter pasteurianus. The primer combination or the kit containing the primer combination can realize species level specific detection of microorganisms, and can be used for qualitatively / quantitatively detecting food and key functional microorganisms in the food fermentation process, so that the dynamic change of a target flora in the fermentation process is quickly and accurately monitored.

The invention relates to the technical field of agricultural microorganisms, in particular to trichoderma asperellum and application thereof.The trichoderma asperellum comprises at least one of the following trichoderma asperellum TA727, the trichoderma asperellum TA727 is preserved in the Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC NO. 63859. The trichoderma asperellum has the advantages that the trichoderma asperellum can be used for preparing the trichoderma asperellum; the trichoderma asperellum is high in sporulation quantity and strong in acid resistance, and has a relatively good pathogenic bacterium inhibition effect under an acidic condition, and volatile substances of the trichoderma asperellum also have an antibacterial effect. The trichoderma asperellum can effectively inhibit pathogenic bacteria in an acid soil environment and soil-borne diseases caused by the pathogenic bacteria, meanwhile, industrial production can be facilitated, and the trichoderma asperellum has wide application prospects.

The invention relates to selenium-enriched pediococcus acidilactici Se34 with anti-oxidation, cholesterol-lowering and anti-tumor effects and application of the selenium-enriched pediococcus acidilactici Se34. The pediococcus acidilactici Se34 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number of the pediococcus acidilactici Se34 is CGMCC No.33933, and the preservation date of the pediococcus acidilactici Se34 is March 24, 2025. The pediococcus acidilactici Se34 provided by the invention integrates excellent probiotic characteristics (acid-resistant bile salt, safe and colonizable), efficient selenium enrichment capacity and remarkably enhanced activity of resisting oxidation, reducing cholesterol and inhibiting specific cancer cell proliferation and metastasis after selenium enrichment. The strain and the selenium-rich product thereof have important application value in the fields of developing functional foods (selenium-rich probiotic products), antioxidant / cholesterol-reducing health-care foods, potential auxiliary anticancer biological preparations or prodrugs and the like.

The invention discloses a strain of Bacteroides ovatus L2 and application of the strain of Bacteroides ovatus L2 in a product for preventing and treating vomitoxin toxicity. The strain is preserved in Guangdong Microbiological Culture Collection Center, the preservation number is GDMCC.No: 67205, and the preservation date is November 4, 2025. The strain is separated and purified from a healthy adult excrement sample, has the characteristics of good acid resistance, cholate resistance, gastrointestinal fluid resistance and the like, and is free of hemolysis and good in safety. The strain can effectively relieve the toxic effect induced by vomitoxin, mainly inhibits inflammatory factors and promotes expression of intestinal barrier functional proteins, can be applied to products of microecologics for preventing and treating DON toxicity, and has a good application prospect.

The invention belongs to the technical field of microorganisms, and particularly relates to application of bifidobacterium bifidum NKUFB3-12 and a composition thereof in preparation of products for relieving allergic diseases and / or regulating intestinal flora caused by allergy. The preservation number of the bifidobacterium bifidum NKUFB3-12 is GDMCC (China General Microbiological Culture Collection Center) No. 64013. The bifidobacterium bifidum NKUFB3-12 disclosed by the invention has excellent functional characteristics of relieving allergic diseases and regulating allergic intestinal flora, and also has excellent probiotic characteristics of acid resistance, cholate resistance and oxidation resistance, and the strain is safe and has no adverse side effect after being taken. The invention provides a composition. The composition comprises bifidobacterium bifidum NKUFB3-12 and 2 '-fucosyllactose (2'-FL), and the NKUFB3-12 is compounded with the 2 '-FL to show an effective inhibition effect on the imbalance of allergic immune response.

The invention belongs to the technical field of microorganisms, and relates to a bifidobacterium breve strain capable of relieving constipation, the strain is bifidobacterium breve Bb-PG-14, the classification name of the strain is Bifidobacterium breve, the preservation number is CGMCC No.31861, the preservation time is September 4, 2024, the preservation unit is China General Microbiological Culture Collection Center, the preservation number is CGMCC No.31861, the preservation number is CGMCC No.31861, the preservation number is CGMCC No.31861, the preservation number is CGMCC No.31861, the preservation number is CGMCC No.31861, and the preservation number is CGMCC No.31861. The address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The strain is separated and purified from excrement of healthy babies, has relatively strong acid resistance, alkali resistance, cholate resistance and artificial simulated gastrointestinal fluid tolerance, and has the characteristics of probiotics.

The invention relates to the technical field of biological breeding, and discloses a composite microecological preparation for adjusting lamb intestinal flora and relieving weaning stress, the composite microecological preparation comprises, by weight, 10-40 parts of enterococcus faecalis powder, 10-40 parts of bacillus subtilis powder and 10-40 parts of saccharomycetes powder, the enterococcus faecalis powder is prepared through a gradient acid-resistant pre-domestication system, and the bacillus subtilis powder is prepared through a gradient acid-resistant pre-domestication system. The bacillus subtilis powder is prepared through an oxygen-controlled induced spore strengthening process, the saccharomycetes powder is prepared through a beta-glucan strengthening reactor, and the three kinds of bacterium powder are mixed under the sterile condition to obtain the bacillus subtilis powder, the saccharomycetes powder and the beta-glucan strengthening reactor. The tolerance, activity and stability of the strain are improved, the controllable and repeatable preparation process of the compound microecological preparation is ensured, and a reliable technical basis is provided for regulating intestinal flora of weaned lambs and relieving weaning stress.

The invention discloses a high-concentration sulfate acid pit water treatment method based on an acid-resistant SRB biological cathode, which comprises the following steps: enabling acid pit water to pass through a grid and then enter a regulating reservoir; introducing the wastewater in the regulating tank into a biological membrane electrochemical device for electrochemical reaction; oxidizing Fe < 2 + > in the wastewater into Fe < 3 + > on a titanium-based IrO2 anode plate and releasing electrons in an anode chamber; in the cathode chamber, an acid-resistant SRB bacterial film loaded on the surface of the carbon felt electrode reduces SO4 < 2-> into S < 2-> by utilizing transferred electrons; cO2 microbubbles are introduced into the liquid in the cathode chamber to react with S < 2-> to generate sulfur precipitates, and the reacted mixed liquid enters a cyclone separator to realize gas-solid separation; and carrying out hydrolysis reaction on Fe < 3 + > in the effluent of the anode chamber to generate Fe (OH) 3 precipitate, mixing the Fe (OH) 3 precipitate with sodium silicate, and calcining to convert the mixture into magnetic alpha-Fe2O3. The method can effectively remove SO4 < 2-> and recover iron sludge.

This invention discloses a strain of Bifidobacterium animalis subspecies TRT-2A with allergy-relieving effects and its extracellular vesicle applications. The strain TRT-2A is deposited at the China Center for Type Culture Collection (CCTCC) on November 21, 2025, with accession number CCTCC NO:M 20252645. This strain is highly acid-resistant and exhibits good tolerance to artificial gastric and intestinal fluids. It effectively inhibits bacteria, particularly Streptococcus pyogenes, Streptococcus agalactiae, and / or Escherichia coli. Experimental results show that this strain can alleviate hormone-dependent dermatitis and effectively improve dermatitis symptoms such as erythema and desquamation. Furthermore, extracellular vesicles prepared using TRT-2A can effectively improve skin elasticity and reduce wrinkles. Therefore, the Bifidobacterium animalis subspecies TRT-2A of this invention has multiple functions and is worthy of further promotion and application.

The invention belongs to the field of bioengineering, and particularly relates to acid-resistant recombinant escherichia coli, a construction method thereof and application of the acid-resistant recombinant escherichia coli in carbon sequestration synthesis of succinic acid in a low-pH environment. Escherichia coli for producing succinic acid is used as a starting strain, overexpression plasmids of carbonic anhydrase genes from two different sources are constructed, and the overexpression plasmids are introduced into the starting strain to obtain an overexpression strain. The two constructed acid-resistant Escherichia coli show good growth and metabolism capabilities in an acid environment, and the yield of succinic acid can reach 80% or above. Through overexpression of carbonic anhydrase, the thalli can still keep high carbonic anhydrase activity in an acid environment, the capacity of the thalli for fixing CO2 to synthesize succinic acid is improved, reduction of acid and alkali consumption in the fermentation and separation process is facilitated, the production cost of succinic acid is reduced, and remarkable social and economic values are achieved.

The invention belongs to the technical field of microbial genetic engineering, and particularly relates to an acid-resistant lactobacillus plantarum engineering bacterium as well as a construction method and application thereof. The recombinant lactobacillus plantarum WCFS1-gadA-speA is obtained by constructing a recombinant expression vector pMG36e-gadA-speA and inducing the expression of two key genes, and the recombinant lactobacillus plantarum WCFS1-gadA-speA is obtained. The analysis of the fermentation capacity of the strain finds that compared with a single gene heterologous expression strain, the recombinant lactobacillus plantarum WCFS1-gadA-speA shows higher acid resistance and apple juice fermentation capacity. The recombinant lactobacillus plantarum WCFS1-gadA-speA with higher strong acid tolerance is obtained through a heterologous expression technology, a strain resource with better performance is provided for the food fermentation industry, and the quality and stability of a fermented product are improved.

The application discloses a high-concentration sulfate radical acid mine water treatment method based on acid-resistant SRB biological cathode, which comprises the following steps: after the acid mine water is filtered through a grid, the acid mine water is fed into an adjusting tank; the wastewater in the adjusting tank is fed into a biological membrane electrochemical device to perform an electrochemical reaction; in an anode chamber, Fe 2+ in the wastewater is oxidized into Fe 3+ and releases electrons; in a cathode chamber, the acid-resistant SRB bacterial membrane loaded on the surface of a carbon felt electrode utilizes the transmitted electrons to reduce SO4 2‑ into S 2‑ ; CO2 micro-bubbles are fed into the liquid in the cathode chamber to react with S 2‑ to generate sulfur precipitate, and the mixed solution after the reaction is fed into a cyclone separator to realize gas-solid separation; Fe 3+ in the water in the anode chamber generates Fe(OH)3 precipitate through a hydrolysis reaction, the Fe(OH)3 precipitate is mixed with sodium silicate, and is calcined to be converted into alpha-Fe2O3 with magnetism. The method can effectively remove SO4 2‑ and recover iron mud.

This invention belongs to the field of wastewater treatment, specifically relating to a composition, treatment agent, and application for treating oily wastewater and oily sludge. The composition comprises component A and component B, with a mass ratio of (40-100):(5-8). Component A consists of cyclodextrin, sodium alginate, and glycerol; component B is acrylamide. This invention uses cyclodextrin, sodium alginate, glycerol, and acrylamide as main components, containing functional groups such as polycarboxyl, polyhydroxy, carbonyl, and amino groups. It utilizes hydrogen bonding, covalent bonding, and electrostatic interactions between these functional groups to achieve rapid treatment of wastewater or sludge. Experiments show that this composition has good acid resistance and adsorption capacity, achieving the removal of oil and suspended solids from acidic oily wastewater and sludge without pre-oxidation or pH adjustment, effectively reducing treatment costs and simplifying the treatment process.

The invention relates to the technical field of microorganisms, in particular to a citrobacter freundii phage and application thereof. The citrobacter freundii phage is a citrobacter freundii phage CFP7ACFGC5 (Citrobacter freundii phage CFP7ACFGC5), the citrobacter freundii phage CFP7ACFGC5 is preserved in the China Center for Type Culture Collection on July 21, 2025, and the number of the citrobacter freundii phage CFP7ACFGC5 is CCTCC (China Center for Type Culture Collection) NO: M20251640. The bacteriophage has a relatively broad-spectrum host range, and can still keep relatively high activity while splitting target bacteria; meanwhile, the bacteriophage is excellent in acid resistance and thermal stability, can meet the application requirements of multiple temperature nodes in production, and has a good application prospect.

The invention relates to the technical field of microorganisms, in particular to a pediococcus acidilactici ZF636 strain and application of the pediococcus acidilactici ZF636 strain. The pediococcus acidilactici strain ZF636 is obtained by screening, is salt-resistant and acid-resistant, has relatively strong ester production capacity, and particularly has high yield of isoamyl acetate; the strain is used for high-salt food fermentation, the content of ester substances, especially isoamyl acetate, of a product and the total amount of volatile compounds can be remarkably increased, meanwhile, the sauce flavor, the ester flavor, the delicate flavor and the strong flavor of the product are improved, the flavor of the product is more balanced and richer, and the flavor and the overall quality of the product are comprehensively improved.

The invention belongs to the technical field of microbial fermentation, and particularly relates to rhodotorula mucilaginosa JQRM001 and application thereof. The invention provides a rhodotorula mucilaginosa JQRM001, the Latin name of the rhodotorula mucilaginosa JQRM001 is Rhodotorula mucilaginosa, the rhodotorula mucilaginosa JQRM001 is preserved in China General Microbiological Culture Collection Center, the address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, the preservation date is July 15, 2025, and the preservation number is CGMCC No.35240. The invention further provides a preparation method of the rhodotorula mucilaginosa. The rhodotorula mucilaginosa JQRM001 not only has excellent performance of high temperature resistance and acid resistance, but also can be used for specifically synthesizing alkylpyrazine flavor substances and remarkably improving the antioxidant activity of plant polysaccharides.

The invention discloses bacillus velezensis B116 and a preparation method of the bacillus velezensis B116. The bacillus velezensis B116 is prepared from bacillus velezensis, bacillus velezensis and bacillus velezensis. Belongs to the technical field of microbial technologies and animal probiotic preparations. The bacillus velezensis B116 obtained by screening has relatively strong biofilm forming ability, intestinal toxin-producing escherichia coli inhibiting activity, good self-aggregation and co-aggregation ability, excellent acid resistance, bile salt resistance, high temperature resistance and artificial gastrointestinal fluid characteristics, has no hemolysis and gelatinase activity, and does not carry drug-resistant genes and virulence genes. Animal experiments show that the strain has no acute toxicity to mice, does not influence visceral organ indexes, blood biochemical indexes and intestinal tissue structures, can significantly reduce the death rate of the mice after ETEC challenge and regulate the intestinal inflammatory factor level, has good probiotic effects and safety, and is suitable for development of feed additives or veterinary probiotics or veterinary drug products.

This invention discloses a strain of *Pediococcus pentosaceus*, FREE-U AP01, and its applications. The strain, FREE-U AP01, has the accession number CGMCC No. 37267. This invention screened and obtained a strain of *Pediococcus pentosaceus*, FREE-U AP01, which, through its excellent temperature adaptability, high survival rate in acid and bile salt tolerance, rapid acid production capacity, and significant antibacterial activity, solves the problems of low survival rate and unstable antibacterial effect of existing probiotics, providing a powerful and high-quality strain resource for microecological preparations and animal feed additives.

The invention relates to the field of agricultural microorganism and heavy metal pollution prevention and control, and provides an acid-stress-resistant metal-resistant cupriavidus strain, application thereof and a fungicide. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.36356, and the strain has the acid-resistant characteristic of stable growth in an environment with the pH value of 3.0-7.0, and has the capability of reducing the concentration of various heavy metal ions such as chromium, manganese, copper, arsenic, cadmium, lead and the like. In a wheat and rice water culture germination test, the strain can remarkably promote root growth and relieve the inhibition effect of stress of various heavy metals on root development, and the removal rate of heavy metal ions can reach a relatively high level. The method can be used for heavy metal pollution treatment and agricultural green and safe production under the acidic condition.

The invention belongs to the field of improvement of acid tolerance and high-density fermentation of probiotics, and particularly relates to a domestication method for improving acid tolerance and fermentation density of lactobacillus plantarum and an obtained strain. The invention provides a domestication method for improving acid tolerance and fermentation density of lactobacillus plantarum, which is characterized in that Lactiplantarum ZJ316 (CCTCC NO: M 208077) is used as a wild strain, the wild strain is domesticated, and the obtained domesticated strain ZJ316PV (Lactiplantarum ZJ316PV) has the preservation number of CGMCC NO.33082. The acclimatization method for improving acid tolerance and fermentation density of lactobacillus plantarum has the advantages that the acclimatization method comprises the following steps: taking Lactiplantarum ZJ316 (CCTCC NO: M 208077) as the wild strain; the acid tolerance of the domesticated strain ZJ316PV is superior to that of the wild strain ZJ316. The invention also provides a method for improving the PV fermentation density of the wild strain ZJ316 and the domesticated strain ZJ316.

The invention belongs to the technical field of microorganisms, and particularly discloses a bacterium-algae symbiotic system repairing preparation and a preparation method and application thereof. In particular to a bacterium-algae symbiotic system repairing preparation, the repairing preparation comprises acid-resistant microalgae and acid-resistant bacteria, the acid-resistant microalgae is chlamydomonas SAG 2486, and the acid-resistant bacteria comprise serratia liquefaciens AN41 and serratia JG-4-2. The invention discloses a bacterium-algae symbiotic system repairing preparation and a preparation method and application thereof, the bacterium-algae symbiotic system repairing preparation naturally improves soil through biological metabolism, and avoids the risks of soil hardening, ion imbalance, secondary salinization and the like caused by a chemical acid regulating agent.

This invention relates to the field of biomedical technology, specifically to a subspecies of Bifidobacterium animalis B189 and its applications. The classification name of this strain is... Bifidobacterium animalum subsp . animal The accession number is CGMCC No. 37052. Bifidobacterium animalis B189 not only exhibits significant antibacterial effects against Helicobacter pylori, but also possesses extremely strong acid and bile resistance, making it valuable for the development of microbial agents to inhibit Helicobacter pylori.

The application discloses a kind of biological hybrid micro machine population oral delivery system of imitating Helicobacter pylori survival mechanism, belong to oral stomach medicine delivery technical field.The system is by active power module (flagellum driven chlamydomonas reinhardtii), environmental regulation module (acid urease modified on algal surface) and multifunctional load module (macrophage membrane wrapped drug-loaded nanoparticles) synergistic assembly through polydopamine (PDA) spatial conformation control layer is formed.Active power module utilizes self-movement ability to penetrate mucus barrier and extend intracavity retention time to 12 hours or more;Environmental regulation module produces local alkalization effect by catalyzing urea hydrolysis, provides chemical shield for system in extremely acid environment of pH 1.0-3.0, protects active power module from gastric acid inactivation;Multifunctional load module is accurately delivered to gastric epithelial cells by inflammation targeting effect, such as CRISPR / Cas9 biological macromolecule, and realizes up to 31.8% in-vivo gene editing efficiency.The application provides a non-invasive, modular active delivery platform for the precise treatment of gastric diseases such as gastritis through the synergistic effect of physical penetration, metabolic reprogramming and gene intervention.

The invention belongs to the technical field of microorganisms, and relates to a lactobacillus paracasei Lpc-14 strain with functions of resisting oxidation and promoting gastrointestinal motility and a preparation method of the lactobacillus paracasei Lpc-14, the lactobacillus paracasei is classified and named as Lactobacillus paracasei, the preservation number of the lactobacillus paracasei is CGMCC No. 29666, the preservation time is January 18, 2024, and the preservation number of the lactobacillus paracasei Lpc-14 is CGMCC No. 29666. The preservation unit is China General Microbiological Culture Collection Center, and the address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The strain is separated and purified from naturally-fermented Chinese sauerkraut fermentation liquor, has the capacity of resisting oxidation and promoting gastrointestinal motility of mice with constipation and improving secretion of gastrointestinal regulating peptides, further has the capacity of resisting acid and alkali and resisting artificial simulated gastrointestinal fluid and bile, and has the characteristics of probiotics.

The invention relates to the technical field of microorganisms, in particular to a lactobacillus plantarum strain LS2 and application thereof.The lactobacillus plantarum strain LS2 is separated from cheese, has the high GABA production capacity and the nitrite degradation capacity, has the antibacterial effect on enteric pathogenic bacteria including escherichia coli, salmonella enteritidis and campylobacter jejuni, and has the advantages that the lactobacillus plantarum strain LS2 is high in GABA production capacity and nitrite degradation capacity; the strain has good antibacterial function, good acid resistance and cholate resistance, and can well survive in artificial intestinal juice and artificial gastric juice; in order to improve the yield of GABA, the accelerant capable of remarkably promoting the growth of the strain LS2 is obtained by researching the accelerant, the highest survival rate of the freeze-dried powder prepared by optimizing the accelerant and the freeze-dried powder can reach 99% or above, the nitrite degradation rate can reach 98% or above, the yield of GABA is improved to 3.3 g / L or above, and the freeze-dried powder is bacterial powder capable of being widely applied.

The present application relates to a kind of genetically engineered bacteria of GLP-1 secretion and its preparation method and application, the genetically engineered bacteria CB-pMTL007-GLP-1 is prepared by integrating GLP-1 gene sequence into the genome of Clostridium butyricum NCU-02 (CGMCC No.25504), the genetically engineered bacteria has the effect of treating type 2 diabetes, reducing high blood pressure and improving Parkinson's disease.The present application uses Clostridium butyricum NCU-02 (CGMCC No.25504) as the host of recombinant expression vector for expressing GLP-1, so that the recombinant plasmid in the protein expression system of the GLP-1 secretion has good genetic stability, also has the same acid resistance, bile salt resistance, antioxidant capacity as wild-type Clostridium butyricum NCU-02 (CGMCC No.25504).

The invention relates to the technical field of probiotics, in particular to intestinal tract source pediococcus acidilactici of Yanqu county in Tibet and fat reducing application thereof. The pediococcus acidilactici disclosed by the invention is preserved in the China General Microbiological Culture Collection Center on April 29, 2025, and the preservation number is CGMCC NO.34406; the strain has the characteristics of acid resistance, cholate resistance and stress resistance, and has high survival rate and planting efficiency; the strain is free of hemolysis, free of toxic factors and high in safety; the strain can secrete short-chain fatty acid and antibacterial peptide, inhibit pathogenic bacteria and promote intestinal barrier repair; short-chain fatty acid generated by metabolism of most of carbon water by the strain can be directly absorbed by intestinal epithelial cells, and can be used as an energy substance to replace partial glucose demand, so that the metabolism energy consumption is reduced, the energy conversion rate of feed is improved, the feed conversion is promoted, and the effects of reducing fat and weight and improving the non-alcoholic fatty liver disease are achieved.