305 results about "Acid tolerance" patented technology

The invention provides an acid-resistant bifidobacterium breve BB8dpH and an application thereof. The nucleotide sequence of the 16SrRNA gene part of the bifidobacterium breve BB8dpH is shown in SEQ ID NO.1, and is stored with the preservation number of CGMCCNo.8370. The bifidobacterium breve BB8dpH is a strain obtained by separating from the dejecta of healthy young people and further screening under the pH 3.2 acid condition. According to the fermentation and culturing process, anaerobic culture at 37 DEG C is carried out in a BL liquid substrate (containing 0.05% cysteine hydrochloride) for 24 hours. The bifidobacterium breve BB8dpH has remarkably better acid resistance than the common strains, and the acid resistance has genetic stability; the bifidobacterium breve BB8dpH has different percent contents of cell membrane fatty acids from the common strains, the average carbon chain length of the bifidobacterium breve BB8dpH is remarkably longer than that of the common strains, and the cell membrane fluidity of the bifidobacterium breve BB8dpH is remarkably lower than that of the common strains; and the bifidobacterium breve BB8dpH is used in the production field of daily fermented food, health-care food and medicines.

The invention discloses preparation and application of Clostridium butyricum and a live Clostridium butyricum preparation; Clostridium butyricum LXKJ-1 is collected in China Center for Type Culture Collection on 24 March, 2016 under CCTCC NO: M201613. A seed medium, fermentation medium formulation, culture conditions for Clostridium butyricum and a production of a preparation of live Clostridium butyricum are also disclosed. The Clostridium butyricum provided herein is tolerant to acids, bases and high temperature, is highly capable of producing butyric acid, can inhibit animal pathogens, such as Escherichia coli, Salmonella, Shigella, Staphylococcus aureus, Listeria monocytogenes, and Clostridium perfringens, and can prevent diarrhea in livestock and poultry due to Escherichia coli, Salmonella and Clostridium perfringens, improving intestinal flora balance, promoting the growth of livestock and poultry, relieving constipation in sows, improving egg weight for layers, improving eggshell quality for layers, and decreasing egg-feed ratio.

The invention belongs to the field of microbes, and concretely relates to a lactic acid bacterium with wide-spectrum bacteriostatic activity and application thereof. The lactic acid bacterium with wide-spectrum bacteriostatic activity is named lactobacillus salivarius GZPH2, is preserved at China Center for Type Culture Collection (CCTCC) since 27th, November, 2014, and has the preservation number of CCTCC M 2014598. The provided lactic acid bacterium lactobacillus salivarius GZPH2 has good bacteriostatic effect on multiple pathogenic bacteria of human and aquaculture bionts, and possesses the characteristics of not generating histamine, no hemolysis, acid resistance, cholate resistance and the like.

The invention provides bacillus licheniformis, wherein the classification name is bacillus licheniformis CY-1, the bacillus licheniformis CY-1 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the address is the Institute of Microbiology of Chinese Academy of Sciences located in 3, Courtyard 1, West Beichen Road, Chaoyang District, Beijing, the postal code is 100101, the accession number is CGMCC NO.12448, and the preservation date is May 13, 2016. The invention further provides applications of bacillus licheniformis in kitchen waste treatment. The bacillus licheniformis has the activities of high-activity protease, amylase and oil hydrolase, can degrade glucose for generating acid, and is a thermophilic bacterium with high salt tolerance and high acid tolerance, and through effective combination of the bacillus licheniformis with a carrier, the weight of the fed kitchen waste is rapidly and efficiently reduced by 85%.

The present invention discloses a strain of acid resistance and high temperature resistance saccharomyces cerevisiae and applications thereof. The saccharomyces cerevisiae is Saccharomyces cerevisiae Sa-10, and is preserved in the China General Microbiological Culture Collection Center, and a preservation number is CGMCC No.6120. According to the present invention, after the Saccharomyces cerevisiae Sa-10 is added to a basal diet of weaned piglets, daily feed intake and daily weight increase of weaned piglets can be significantly improved, and a diarrhea index can be reduced; and the Saccharomyces cerevisiae Sa-10 provides important significance and broad application prospects in the field of animal feed additive preparation or animal production performance improvement.

The invention provides lactobacillus reuteri. The lactobacillus reuteri was deposited at the General Microbiology Center of China Microbiological Culture Collection and Management Committee on December 14, 2017 with the deposition number of CGMCC No. 15062. The lactobacillus reuteri has broad-spectrum bacteriostatic effect, good adhesion property and strong acid resistance and bile salt resistance, is obviously superior to an existing commercial strain, and is a newly isolated and discovered active bacterium with a good probiotic function.

A fire-resistant and acid-proof alpha-amylase and its production are disclosed. The process is carried out by separating precursor-alpha-amylase gene from lichenized bacillosporin, mutating for L134 and S320 amino acid residues and high-efficient expressing alpha-amylase mutant in bacterium. It achieves better safety, expression and acid stability, and more yield.

The invention relates to the field of producing highly efficient and low-cost microbiological feed proteins based on the three-stage fermentation of vinegar residue and miscellaneous meal by using probiotics. The vinegar residue used as a main carbon source and the cottonseed meal used as a main nitrogen source as well as a partial carbon source are subjected to the first-stage fermentation pretreatment by using food-grade mould, the product is subjected to the second-stage fermentation by using bacillus and yeast as zymocytes and adding partial cottonseeds, and finally the product is added with partial soybean meal and rapeseed meal used as a nitrogen source as well as a partial carbon source, inoculated with lactobacillus and acid-resisting bifidobacterium and uniformly mixed, and then the product is subpackaged in one-way membrane bio-bags for sealed storage, namely the finished products are obtained by the three-stage fermentation. The microbiological feed proteins are extremely low in production cost and can be used as a feed component, and the adding amount reaches 5 to 40 percent. Because the finished products do not need drying treatment, the finished products have more viable bacteria as well as enzymes, few other bacteria and an obvious feeding effect; moreover, the microbiological feed proteins also have the advantages of greatly improving the utilization rate of livestock and poultry feed, increasing yield, improving intestinal environment, improving immunity and resistance, replacing antibiotics and improving quality of animal products.

The invention discloses a Lactobacillus johnsonii, and a preparation and application thereof, and discloses an optimal culture medium for the strain and a preparation method of a freeze-dried preparation of the strain. The microbial preservation number of the Lactobacillus johnsonii ZLJ010 is CGMCC No.5762. The strain is definite in hereditary basis, and has the characteristics of good growth performance and high acid production, acid resistance, heat resistance and bacterium inhibition capabilities. When being used as a feed additive for sows, the freeze-dried preparation of the strain can improve the intestinal tract health of the sows, reduce the number of harmful bacteria in feces, enhance the body immunity and increase the production performance of the sows.

The invention discloses enterococcus faecium, a culture medium thereof for high-density solid-state fermentation, and a high-density solid-state fermentation method, wherein the preservation number of the bacterial strain is CGMCC No.11155. Enterococcus faecium has the excellent performances of inhibiting bacteria, resisting acid and cholate, and the like, is tolerant to low moisture, and has relatively long shelf life under solid state fermentation condition. The culture medium contains wheat bran, bean pulp, cane molasses, activated carbon, calcium carbonate and beta- cyclodextrine. In the preparation technology, lactobacillus is inoculated into the culture medium for fermentation, and then drying is carried out at 45-65 DEG C, and thus obtaining a fermentation product. Compared with the like enterococcus faecium strains, the strain and the fermentation product provided by the invention have the advantages of high concentration of viable organisms, long shelf life and the like. The solid-state fermentation product of the strain provided by the invention can significantly improve the daily gain, the survival rate and the feed conversion ratio of nursery pigs, is obvious in economic benefit, and can be taken as a feed additive.

The invention relates to a method for evaluating acid tolerance of concrete. The method includes: 1), preparing concrete strength testing pieces and acidization depth testing pieces; 2), maintaining the testing pieces in a standard maintaining chamber for 26d, dry-maintaining the testing pieces for 2d, disposing the testing pieces into mixed acid liquid for soaking, taking out the testing pieces for drying for 2d after being soaked for 5d each time, measuring compressive strength X (t) of a group of the concrete strength testing pieces and width b (t) of un-corroded parts of a group of the acidization depth testing pieces after four circulating periods of soaking-drying; 3), calculating concrete acid-corrosion-resistant coefficient Q according to the obtained compressive strength X (t); 4), calculating concrete acidization depth d according to the width b (t) of the un-corroded parts; 5), dividing concrete corrosion-resistant grades into I, II, III and IV according to data conditions of Q and d, and evaluating acid-corrosion-resistant grade, acid resistance and suitable environment acting grade of concrete according to the concrete corrosion-resistant grades.

The method uses the anaerobic glove case and many modified culture medium to screen the anaerobic bacteria which possesses the highly effective character of degrading chlorophenol and acid resistance. The method comprises the following steps: A. screening the anaerobic particle sludge; B. segregation and purifying bacteria; C. primary screening the anaerobic bacteria degrading chlorophenol; D. second screening: segregation and purifying with the solid-liquid culture medium, until the cell shape being the single thallus. The operation is simple, and the theory meaning and economic value are important. The method provides the theory basis for anaerobic biological treatment process of chlorophenol waste water. The method can be used to separate, screen and culture the chlorophenol degradation bacterium, and provide the good screening method for researching chlorophenol degradation rule and interaction between function bacteria.

The present invention relates to the preparation process of magnetic microballoon of composite polysaccharide. The magnetic microballoon with certain magnetic response property is prepared with tamarind gelatin or sweet wormwood gelatin and chitosan as material, ferroferric iron as magnetofluid, glutaraldehyde as cross-linking agent and o-aminobenzene sulfonic acid as aminating agent. The magnetic microballoon has surface functional radical of -OSO2(C6H4)N-C(CH2)3CHO. The magnetic microballoon of composite polysaccharide has less volume swelling, high acid tolerance and low cost.

The invention relates to a compound biological agent for enabling an acidified marsh gas fermentation system to restore gas production and a preparation method thereof. The compound biological agent contains acidophilus acid-resistant methane-producing bacteria, syntrophic acetic acid oxidation bacteria and minor elements. The compound biological agent enables an acidified marsh gas fermentation system to quickly restore normal gas production without adding alkaline substances or performing massive material discharging or changing, thus ensuring continuous stable gas production of the marsh gas fermentation system. The invention also provides a preparation method of the compound biological agent, which comprises the following steps: under anaerobic conditions, mixing a mixed bacterium solution of acidophilus acid-resistant methane-producing bacteria and a mixed bacterium solution of syntrophic acetic acid oxidation bacteria to obtain a compound bacterium solution, concentrating until the total concentration of cells of the acidophilus acid-resistant methane-producing bacteria and the syntrophic acetic acid oxidation bacteria is at least 1*10<9>/mL, and then adding the minor elements; and packaging, preserving at low temperature, and preparing into the liquid compound biological agent for enabling an acidified marsh gas fermentation system to restore gas production. A solid agent can be further prepared by stirring and mixing with auxiliary materials and then performing anaerobic drying.

The application provides a lactobacillus paracasei PC-01 isolated from sour yak milk in Longren Township, Dangxiong County, Lhasa, Tibet, wherein the microbial preservation number of the lactobacillus paracasei PC-01 is CGMCC No.17537. The Lactobacillus paracasei PC-01 can improve oral health flora imbalance, has probiotics with better acid tolerance and bile salt tolerance, has probiotic characteristics, can be directly taken as a preparation for improving oral microecological environment, or as a raw material to prepare a microbial antagonist for pathological states in oral cavity, wherein the pathological state microorganisms comprise at least one of streptococcus mutans, Porphyromonas gingivalis, Treponema denticola, Genus Prevotella and Fusobacterium nucleatum.

The invention provides a preparation method of modified starch nanocrystal, belonging to the technical field of the development of modified starch. The principle of the preparation method provided by the inversion is based on the situation that the difference of the acid tolerance is caused by the hemi-crystal capability of a starch structure, an amorphous region of starch particles is restrictively hydrolyzed by dilute acid, and the acid-resistant starch microcrystal particles can be formed, wherein the sizes of the acid-resistant starch microcrystal particles are 10-100nm. The particles are ultramicro, so that the particles are easily absorbed onto an oil-water interface, and have a certain surface activity, and the surface activity of the particles can be greatly improved after the particles are modified on surfaces in an esterification way by the DDSA (dodecenly succinic anhydride) through an enzymic method. 0.5% of the modified nanocrystal is added, so that a water and weak-polarity methylbenzene (1:1) oil-water system can form stable O/W type emulsion, the emulsifying capacity reaches 76%, and the median diameter of the emulsion D50=55 microns; after the emulsion is placed for four months under room temperature (0.2% of sodium azide bacteriostat is added), the particle size distribution of the emulsion can not be obviously changed. The modified starch nanocrystal prepared by the preparation method provided by the invention is high in emulsifying capacity, and high in emulsifying stability, thereby being wide in industrial application prospect.

The invention discloses a bacillus licheniformis drug combination and the preparation, which comprises pharmaceutical live bacteria powder and suitable accessories. The bacillus licheniformis drug combination is characterized in that: living bacteria number of bacillus licheniformis for each g of the combination is 0.5 to 2.5 billion and preferred number is 1 to 2 billion; volume per weight for each suitable accessories are dispersing agent 70% to 95%, adhesive 1% to 20%, disintegrating agent 1% to 6%, lubricant 0.1% to 1% and coating liquid1% to 10% respectively. The drug combination is specific release preparation using bacillus licheniformis as active ingredient. Enteric-coated preparation is preferred and enteric-coated tablet, enteric-coated capsule and enteric-coated granule are more preferred. Survival rate experiments shows that: only 2.17% survives after live bacteria powder is orally taken for 4 hours and 93.7% to 95.2% survives after the preparation is orally taken. So the bacillus licheniformis drug combination has an advantage of overcoming the drawbacks of acid nonresistance and no survival for microecology liver bacteria under gastric-acid environment.