476 results about "Acid tolerance" patented technology

The invention discloses acid-resistant pichia kudriavzevii and an application of the acid-resistant pichia kudriavzevii. The acid-resistant pichia kudriavzevii is Pichia kudriavzevii S9 and is preserved in the Guangdong Microbial Culture Collection Center on September 20, 2024, and the preservation number of the acid-resistant pichia kudriavzevii S9 is GDMCC.No: 65148. The acid-resistant pichia kudriavzevii S9 has the advantages that the acid-resistant pichia kudriavzevii S9 is used for According to the invention, a low-pH-resistant pichia kudriavzevii strain is screened from surface microorganisms of grapes, and the pichia kudriavzevii strain can tolerate an environment with pH of 1.8 and ferment organic acid under the condition of not adding a neutralizing agent; besides, genetic modification is carried out based on pichia kudriavzevii, including knocking out succinic acid degradation related genes, knocking out ethanol and glycerol production pathways and enhancing succinic acid transporter expression, so that engineering bacteria for high-yield succinic acid are obtained, and the fermentation yield of succinic acid reaches 31.74 g / L under the condition that a neutralizer is not added.

The invention discloses lactococcus gasseri C6a2 and application thereof. The preservation number of the lactococcus gasseri C6a2 is CGMCC (China General Microbiological Culture Collection Center) NO: 31024; the 16S rRNA (ribosomal Ribonucleic Acid) gene sequence of the lactococcus gasseri C6a2 is as shown in SEQ ID NO: 1. The bacterial strain is a macrobrachium rosenbergii intestinal absolute dominant bacterium, has a typical probiotic function, is high in growth speed and activity, can quickly enter a logarithmic phase, has relatively high acid production and acid resistance, can secrete lipase, has relatively high bacteriostatic activity on staphylococcus aureus, aeromonas hydrophila and aeromonas veronii, and can be used for preparing the macrobrachium rosenbergii. The bacterium can be used for preparing a special probiotic preparation for macrobrachium rosenbergii, so that the sustainable development of the macrobrachium rosenbergii industry in China is promoted.

The invention belongs to the technical field of microorganisms, and particularly relates to a plant lactobacillus B534 strain and application thereof in preparation of functional products beneficial to sleep improvement and emotion regulation. The lactobacillus plantarum B534 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the lactobacillus plantarum B534 is CGMCC No.33031. The lactobacillus plantarum B534 is named as lactobacillus plantarum B534. The plant lactobacillus B534 provided by the invention is a natural strain and has food safety; the lactobacillus plantarum B534 has relatively strong acetylcholin esterase inhibition activity, and also has relatively strong capability of synthesizing and secreting GABA (gamma-aminobutyric acid), 3-HB (3-hydroxybutyric acid) and 5-HT (5-hydroxytryptamine); meanwhile, the plant lactobacillus B534 has relatively strong acid resistance and cholate resistance and also has relatively strong tolerance to simulated gastrointestinal fluid, so that the plant lactobacillus B534 has relatively good intestinal colonization ability and has important application value in the fields of products beneficial to sleep improvement, emotion regulation products and the like.

The invention discloses enterobacter rolvestris and application thereof, the classification name of the enterobacter rolvestris is enterobacter rolvestris, the enterobacter rolvestris is preserved in China General Microbiological Culture Collection Center on July 12, 2024, the preservation number is CGMCC No.31282, and the address of the preservation unit is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The strain is higher in salt tolerance which can reach 8.5%, and has acid resistance, alkali resistance and higher IAA (indoleacetic acid) production capacity.

The invention relates to pediococcus pentosaceus with a cholesterol lowering function and application thereof, and belongs to the technical field of microorganisms. The invention discloses pediococcus pentosaceus with a cholesterol lowering function and application of the pediococcus pentosaceus. The pediococcus pentosaceus R5 (GDMCC No.65659) is separated from special fermented food stinky acid in Guizhou. The in-vitro cholesterol removal rate of the pediococcus pentosaceus R5 can reach 59%, and the pediococcus pentosaceus R5 has the characteristics of acid / cholate resistance, no hemolysis and sensitivity to common antibiotics; an in-vivo lipid-lowering experiment is carried out on a hyperlipidemia model mouse, and the result shows that the strain R5 can remarkably improve the in-vivo dyslipidemia state of the mouse, reduce liver fat accumulation and protect the liver at the same time, and can be used for developing lipid-lowering related probiotic functional products. The strain R5 provided by the invention provides a natural and safe probiotic for preventing and / or treating hyperlipemia.

The invention relates to acid-resistant sulfate reducing bacteria and application thereof, and belongs to the technical field of microorganisms. The acid-resistant sulfate reducing bacterium disclosed by the invention is Desulfovibrio desulfuricans QY411, and is preserved in the Guangdong Microbial Culture Collection Center on March 14, 2024, and the preservation number of the acid-resistant sulfate reducing bacterium is GDMCC No: 64412. The acid-resistant sulfate reducing bacterium provided by the invention can survive in an environment with the pH value of 4, and can be applied to ecological reconstruction of acid tailings and waste dump soil, the pH values of mining wasteland soil and surface water after ecological reconstruction are remarkably increased, the concentration of SO4 < 2-> in the soil is remarkably reduced, the acidification process in the mining wasteland soil is effectively inhibited, and the acid-resistant sulfate reducing bacterium has the advantages of being safe and reliable. Therefore, the acidification process in the mining wasteland is effectively controlled. Meanwhile, soil heavy metal pollution is reduced, and vegetation growth is recovered.

The invention provides a plant lactobacillus YH001 with bacteriostatic activity as well as a screening method and application of the plant lactobacillus YH001. The phytobacterium plantarum YH001 has the taxonomic name of Lactiplantibacillus plantarum, is preserved in the Guangdong Microbial Culture Collection Center, and has the preservation number of GDMCC No: 65693, the preservation number of Lactiplantibacillus plantarum YH001 is CGMCC NO: 65693, and the preservation number is CGMCC NO: 65693. The preservation date is December 30, 2024. The strain has excellent biological characteristics of no hemolysis, acid resistance, cholate resistance and the like, and has wide application potential in the fields of food, medicine and the like. The fermentation supernatant still keeps high bacteriostatic activity after being treated at a high temperature of 121 DEG C, and shows excellent thermal stability. Therefore, the strain has a wide application prospect in research and development of antibacterial agents, food leavening agents and preservative products.

The invention relates to the technical field of biological medicine, and discloses a formula of compound probiotic enzyme powder for promoting gastrointestinal microecological balance and nutrient absorption, by constructing a cross feeding system of bifidobacteria, lactobacillus and butyric acid bacteria and combining a stomach-intestine stage enzyme activity gradient adaptation strategy and a microenvironment response type double-layer embedding technology, the gastrointestinal microecological balance and nutrient absorption can be promoted, and the gastrointestinal microecological balance and nutrient absorption can be promoted. And cross-scale synergy of flora metabolism, substrate conversion and targeted release is realized. Wherein the sodium alginate-pectin composite embedding structure forms a pH buffer micro-area in a gastric acid environment, and proton pump gene expression of the acid-resistant strain is synchronously triggered; the prebiotics compounding and step-by-step enzymolysis process breaks through a closed-loop path of fiber degradation-flora proliferation-short-chain fatty acid generation. Compared with a traditional single-strain intervention scheme, the method has the advantages that double limitations of flora function splitting and process isolation are broken through, and systematic technical advantages are formed in the aspects of improving flora colonization stability, enhancing metabolite conversion efficiency and inhibiting pathogenic bacterium colonization.

The present invention discloses an application of a salt-tolerant heterotrophic nitrification-aerobic denitrifying bacteria in wastewater treatment and plant growth promotion. The present invention provides an application of Glutamicibacter nicotianae XH-220 or a bacterial agent containing the Glutamicibacter nicotianae XH-220 in nitrogen-containing wastewater and cucumber seedling growth promotion treatment; the Glutamicibacter nicotianae XH-220 is numbered ACCC 62208 in the Agricultural Microbiology Center of the China Microbiological Culture Collection Administration. The strain has acid resistance, salt resistance and heavy metal resistance, and also has a plant growth promotion effect. The present invention has important application prospects in the fields of sewage treatment and plant growth promotion.

The invention discloses a bacillus thuringiensis L3 and an application thereof. The preservation number of the bacillus thuringiensis L3 is CCTCC (China Center for Type Culture Collection) NO: M 2025487. The gene sequence of the 16S rDNA of the bacillus thuringiensis L3 is as shown in SEQ ID NO: 1. Under different adversity stress conditions, the bacillus thuringiensis L3 shows a certain stress resistance potential. Research finds that the bacillus thuringiensis L3 has certain acid resistance, high temperature resistance, cholate resistance and drought resistance, and has strong alkali resistance.

The present invention discloses a Lactobacillus plantarum and its application in the preparation of functional preparations for preventing and treating inflammatory bowel disease, as well as antioxidant, anti-inflammatory and antibacterial functions. It has excellent ability to scavenge reactive oxygen species, as well as good probiotic characteristics such as acid tolerance, bile salt tolerance, hydrophobicity, self-aggregation ability and inhibition of common human pathogenic bacteria. At the same time, it can improve intestinal inflammation, has a preventive and therapeutic effect on inflammatory bowel disease, and has high biological safety. It can be used as a probiotic preparation to relieve inflammatory bowel disease, improve intestinal mucosal damage and colon inflammation, providing a new strategy for the prevention and treatment of inflammatory bowel disease and having potential clinical application prospects.

The invention relates to the technical field of microorganisms, in particular to animal-derived pediococcus acidilactici and application thereof. The pediococcus acidilactici is classified and named as pediococcus acidilactici, the strain number of the pediococcus acidilactici is 12NN1, the pediococcus acidilactici is preserved in Guangdong Microbial Culture Collection Center on January 2, 2025, and the preservation number of the pediococcus acidilactici is GDMCC No: 65706. The strain is resistant to acid and cholate, has a remarkable inhibiting effect on growth and reproduction of pathogenic escherichia coli and staphylococcus aureus, is safe and non-toxic, does not have pathogenicity, and provides beneficial strain resources and biological materials for development of corresponding microbial preparations. Therefore, the compound can be used for preparing a biocontrol preparation for inhibiting the growth of pathogenic bacteria, preparing animal feed and preparing a preparation for preventing, improving and / or treating gastrointestinal tract pathogenic bacteria infection, so that the compound has a wide application prospect.

This application relates to the field of food microbiology, and specifically discloses a Lactobacillus plantarum capable of producing 5-methyltetrahydrofolic acid, its fermentation method and application. A Lactobacillus plantarum inm30-LP capable of producing 5-methyltetrahydrofolic acid, with a preservation date of December 26, 2022, and the preservation unit is the General Microbiology Center of the China Committee for Culture Collection of Microorganisms, and the preservation number is CGMCC No. 26278. This application is based on the milk curd in the Gannan Tibetan area as the food source, isolates probiotics, and screens out Lactobacillus plantarum with high folic acid production from them. Moreover, the strain has obvious advantages over other lactic acid bacteria in terms of acid resistance and bile salt resistance, is suitable for growing in the gastrointestinal environment, has the ability to proliferate, has no antibiotic resistance, has antibacterial activity, and can produce bacteriocin.

The invention belongs to the technical field of microorganisms, and particularly relates to saccharomyces cerevisiae, a fungicide, application and a screening method. The invention provides a saccharomyces cerevisiae strain DXMC847, which is classified and named as saccharomyces cerevisiae, and is preserved in China Center for Type Culture Collection on November 18, 2024, the preservation address is Wuhan University, Wuhan, China, and the preservation number is CCTCC NO: M 20242592. The saccharomyces cerevisiae strain BY4741 is used as an original strain, and a method of combining ultraviolet mutagenesis with flow sorting is utilized to realize high-throughput screening of acid-resistant strains, so that the acid-resistant strains can be efficiently screened, and the yield of the acid-resistant strains is improved. And finally obtaining the acid-resistant saccharomyces cerevisiae DXMC847. The result of the embodiment shows that the content of pyruvic acid, L-malic acid and succinic acid in the fermentation liquor of the saccharomyces cerevisiae DXMC847 is obviously improved compared with that of the saccharomyces cerevisiae BY4741.

The invention relates to the technical field of agriculture, provides a red soil hardening reduction conditioner based on modified straw adsorption carbon as well as a preparation method and application thereof, and solves the problems that an existing red soil hardening conditioner is poor in soil improvement effect and does not have a slow release function. Comprising the following steps: (1) preparing oyster shell-biochar composite powder; (2) loading functional components; (3) directional immobilization of microorganisms; (4) coating a slow-release layer; (5) activating treatment; the red soil hardening reduction conditioner is prepared from the following raw materials in parts by weight: 80 to 100 parts of oyster shell powder, 100 to 200 parts of modified straw adsorption carbon, 10 to 30 parts of rice hull powder, 30 to 50 parts of nano dihydrate gypsum, 100 to 200 parts of a polyglutamic acid solution, 10 to 50 parts of acid-resistant bacillus, 15 to 30 parts of sodium alginate, 30 to 60 parts of humic acid, 10 to 30 parts of carbide slag and 30 to 60 parts of attapulgite.

The invention discloses a salt-resistant and acid-resistant yeast fungus capable of efficiently degrading benzene series and application of the yeast fungus, the yeast fungus is Barnettozyma hawaiiensis, and the preservation number is CCTCC (China Center for Type Culture Collection) M 20241391. The yeast strain can keep activity in a high-salinity and acidic environment, obviously reduces the content of organic matters in high-salinity wastewater, and shows efficient degradation capacity on benzene series, especially benzaldehyde, in the high-salinity acidic wastewater. The method is especially suitable for treating high-salt or acidic organic wastewater and refractory industrial organic wastewater containing benzene series such as shale gas flowback fluid. An effective bacterium source is provided for biochemical treatment of high-salt acidic refractory industrial organic wastewater containing benzene series and the like.

The invention discloses a porcine epidemic diarrhea virus (PEDV) infection resistant Min pig source lactobacillus salivarius LSM231 and application thereof, and belongs to the technical field of microorganisms. The Min pig source lactobacillus salivarius is named as Ligilbacillus salivarius LSM231 and is preserved in the China Center for Type Culture Collection, the preservation number is CCTCC NO: M 20242330, and the preservation time is October 25, 2024. The Min pig source lactobacillus salivarius has the advantages that the Min pig source lactobacillus salivarius is named as Ligilbacillus salivarius LSM231 and is preserved in the China Center for Type Culture Collection (CCTCC); the screened Min pig source lactobacillus salivarius is symbiotic probiotics in intestinal tracts and is high in safety performance, and experimental results show that the Min pig source lactobacillus salivarius LSM231 has relatively good antibacterial, acid-resistant and cholate-resistant characteristics, has a remarkable PEDV (porcine epidemic diarrhea virus) resisting effect, and can relieve clinical symptoms caused by PEDV infection and promote growth of piglets, so that the Min pig source lactobacillus salivarius LSM231 can be used for preventing and treating the porcine epidemic diarrhea. The compound can replace antibiotics to be used for preparing a micro-ecological preparation for resisting PEDV (porcine epidemic diarrhea virus) infection.

The invention relates to the field of microorganisms, and discloses a Lactococcus lactis subsp. Lactis VB346 which is preserved in the China Center for Type Culture Collection on September 20, 2024, and the preservation number is CCTCC NO: M 20242013. The invention further discloses a preparation method of the Lactococcus lactis subsp. Lactis VB346. The lactococcus lactis subsp. Lactis VB346 disclosed by the invention has the capability of efficiently degrading purine nucleoside, and can decompose purine nucleoside in ingested food in intestinal tracts, so that the absorption amount of purine nucleoside by small intestines is reduced. The polypeptide has the ability of degrading bilirubin, and can be used for treating or improving hyperbilirubinemia, or improving liver and gall injury caused by hyperbilirubin; meanwhile, the acid resistance required by survival in the stomach, the cholate tolerance required by survival in the intestinal tract and the stable survival ability in the intestinal tract are achieved, and the bacillus subtilis has huge application prospects in preparation of drugs, microbial agents, health care products or food.

The invention relates to bacillus subtilis Y32, and the preservation number of the bacillus subtilis Y32 is CGMCC (China General Microbiological Culture Collection Center) No.34819. The strain can produce xylanase with high temperature resistance and high stability, various hydrolases such as beta-mannase, protease and the like, exopolysaccharides and botrytis cinerea inhibition, and is resistant to acid, swine bile salt and 10% NaCl. The optimum temperature of xylanase produced by the strain is 55 DEG C, the optimum pH value is 6.0, under the conditions, the half-life period is 12.5 h, the relative enzyme activity is still kept above 80% after incubation for 3 h, and the xylanase has good thermal stability. The strain can also improve the yield of cucumbers and pinellia ternate, and can also improve the laying rate of 70-week-old hyline brown laying hens from 70.4% to 90.1%. The strain has a good application prospect in the fields of agriculture, food processing and cosmetic production.

The invention discloses a preparation method of a micro-ecological bacterium enzyme preparation and application of the micro-ecological bacterium enzyme preparation in a feed additive, and belongs to the field of preparation of a fermented feed by combining a compound microorganism strain with a biological enzyme. Centrifugally separating and mixing bacterial liquid cultured by the acid-resistant zymophyte to obtain a mixed bacterial precipitate, and freeze-drying the mixed bacterial precipitate to obtain mixed bacterial powder; freezing a mixed enzyme solution formed by mixing papain, bromelain and cellulase to obtain mixed enzyme powder; and mixing the mixed bacterial powder and the mixed enzyme powder to form the micro-ecological bacterial enzyme preparation. Mixing and stirring the micro-ecological bacterium enzyme preparation, basal feed and water, and fermenting to obtain a fermentation mixture; adjusting the pH value of the fermentation mixture with hydrochloric acid, mixing with aldehyde chitosan, stirring and reacting to obtain a cross-linked fermentation product, and drying the cross-linked fermentation product with hot air to obtain the solid fermentation feed.

The invention belongs to the technical field of probiotics, and particularly relates to pediococcus acidilactici with acid resistance, bile salt resistance and antibacterial activity and application of the pediococcus acidilactici. The pediococcus acidilactici TR19 has the preservation number of CGMCC NO.31330 and is classified and named as pediococcus acidilactici according to microorganisms. The strain has good acid resistance and bile salt resistance, and can be used for preparing probiotics for preventing and treating bacteria. Meanwhile, the strain has a relatively strong bacteriostatic effect on escherichia coli, bacillus cereus, staphylococcus aureus and salmonella typhimurium, in addition, the strain has a relatively good degradation capability on nitrite and cholesterol, and the applicability is relatively strong.

The invention relates to the field of microorganisms, in particular to cat milk-derived pediococcus acidilactici and application thereof. The cat milk source pediococcus acidilactici provided by the invention is preserved in the China General Microbiological Culture Collection Center on July 1, 2024, the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No. 3, Beichen West Road, Chaoyang District, Beijing, China, and the preservation number is CGMCC NO.31006. The cat milk-derived pediococcus acidilactici provided by the invention is obtained by screening from healthy cat milk, has relatively strong acid resistance and bile salt resistance, is extremely strong in intestinal tract adhesion capacity, has a good inhibition effect on common pathogenic bacteria, is sensitive to various antibiotics, has good probiotic potential, and can be used for preparing a feed additive for the cat milk-derived pediococcus acidilactici. The invention provides a new candidate strain for pet food, health care and treatment products or probiotic preparations, and has wide application prospects in the aspects of preparation of pet food, health care products, medicines and the like.

The invention belongs to the technical field of microorganisms, and particularly relates to bacillus cereus as well as a culture and application thereof. The preservation number of the bacillus cereus AK-LY1 is CCTCC (China Center For Type Culture Collection) NO: M 2025756. The bacillus cereus AK-LY1 disclosed by the invention is excellent in trypsin resistance, good in cholate resistance, acid resistance and high temperature resistance, good in inhibition performance on antibiotics such as strong penicillin and chloramphenicol, and relatively good in adhesion capacity in intestinal tracts, and provides a relatively good microecological preparation reference basis for expanding a bacillus cereus resource library and applying the bacillus cereus.

The invention discloses a biological soil conditioner containing mineral powder and a preparation method thereof, and belongs to the technical field of microbial soil conditioners. The conditioner is prepared from the following raw materials in parts by weight: 20 to 40 parts of compound microorganism bacterium liquid, 10 to 20 parts of mineral powder and 15 to 20 parts of auxiliary material carrier. According to the invention, two high-acid-resistance growth-promoting bacterial fungal microorganisms are screened, compounded and loaded into the high-activity rice hull biochar, and all the raw materials are matched with one another, so that the aluminum pollution of the acid-aluminum soil can be improved, the soil fertility can be improved, the growth of plants can be promoted, the microbial community structure of the soil can be enriched, and the application prospect is great.

The invention relates to the technical field of soil conditioners, in particular to a soil conditioner for soil acidification treatment as well as a preparation method and application of the soil conditioner. Comprising the following components in parts by weight: 20-35 parts of red mud, 10-15 parts of a humic acid-sodium alginate compound, 10-15 parts of zeolite, 5-8 parts of a silicon-calcium-magnesium fertilizer, 4-8 parts of an acid-proof bacterial agent and 10-15 parts of a bio-based adhesive. The acidified soil treatment effect can be better, and the effect is more durable.

This invention belongs to the field of microbial technology, specifically relating to a strain of *Pediococcus pentosaceus* SUN02 with acetylcholinesterase inhibitory activity and its applications. This *Pediococcus pentosaceus* SUN02 is deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 29705. The *Pediococcus pentosaceus* SUN02 provided by this invention exhibits strong acid and bile salt resistance, good tolerance in simulated gastric and intestinal fluids, and can successfully reach the human intestine. *Pediococcus pentosaceus* SUN02, in its bacterial suspension and inactivated cells, possesses strong acetylcholinesterase inhibitory activity, produces γ-aminobutyric acid (GABA), and has antioxidant and other probiotic functions. Its application in probiotic products may help improve Alzheimer's disease, enhance learning and memory abilities, regulate mood, and improve sleep.

The invention provides low-temperature alginate lyase and application thereof, namely alginate lyase with low-temperature catalytic activity, which is separated from marine-derived cold monad bacteria, and the amino acid sequence of the alginate lyase is SEQ ID NO: 1; the amino acid sequence of the alginate lyase after the signal peptide is removed is SEQ ID NO: 3. The algin lyase Alg3889 provided by the invention has the optimal reaction temperature of about 10-16 DEG C, has good temperature stability, and still keeps more than 70% of enzyme activity after being incubated at 0-70 DEG C for 1 hour; the optimum pH value of the strain is 8.0, after the strain is incubated in a buffer system with the pH value of 3.0-10.6 for 12 h, about 90% of the highest enzyme activity can be kept, and the strain has high acid resistance and alkali resistance. The degradation product of the alginate oligosaccharide is alginate oligosaccharide with the polymerization degree of 2-6, and the alginate oligosaccharide has high application value.

The invention provides acid-resistant and cholate-resistant lactobacillus plantarum and application thereof, and relates to the technical field of microorganisms. The invention provides a strain with the capacity of degrading nucleic acid and inhibiting xanthine oxidase, namely lactobacillus plantarum HXJS3-1, and the lactobacillus plantarum HXJS3-1 has the effects of reducing the uric acid level and preventing and treating hyperuricemia and gout. In addition, the lactobacillus plantarum HXJS3-1 also has a certain anti-inflammatory effect and an effect of enhancing immunity. And a novel probiotic or medicine material is provided for treatment of hyperuricemia and related diseases thereof. The lactobacillus plantarum HXJS3-1 also has the characteristics of acid resistance, cholate resistance and no influence of existence of a carbon source and a nitrogen source, so that the lactobacillus plantarum HXJS3-1 can be widely applied to various scenes.

The invention discloses a lactobacillus johnsonii and related products and applications thereof, and relates to the field of microorganisms. The Lactobacillus johnsonii GJ231 provided by the invention has a preservation number of CCTCC NO: M 2023981. Compared with other lactobacillus johnsonii, GJ231 is acid-resistant and bile-resistant, has a higher survival rate in the intestine, has a better antibacterial effect on a variety of pathogens, and can be used to prevent and treat related diseases caused by pathogens; secondly, GJ231 has a better high temperature tolerance and has a higher survival rate in the process of spraying and preparing pet food; furthermore, GJ231 also has a strong antioxidant capacity, can reduce the peroxidation reaction caused by oxidative stress, and can be used for anti-aging and anti-oxidation; in addition, GJ231 also has a bacteriocin gene cluster, which may be used to produce bacteriocin, and has the potential to replace antibiotics and food preservation.

The invention relates to the technical field of microbial fermentation, and specifically discloses a low-pyruvate xanthan gum and a preparation method thereof. The preparation method of the low-pyruvate xanthan gum comprises the following steps: S1, after activating xanthomonas by using an activation culture medium, transferring the activated strain into a seed culture medium for cultivation to obtain a seed liquid, inoculating the seed liquid into a fermentation culture medium, and obtaining a fermentation liquid after the cultivation is completed; S2, adding a treatment agent to the fermentation liquid obtained in step S1, stirring it, heating it to obtain a treatment liquid, extracting it with ethanol after cooling, drying it, and crushing it to obtain the treated liquid; the obtained low-pyruvate xanthan gum has excellent synergy, high temperature resistance, acid resistance, salt resistance and other properties, and has good product stability; the culture medium of the invention has simple components, a simple preparation method, saves time compared with mutating strains and improving strains, saves cost compared with optimizing culture medium components and adjusting culture control conditions, and is suitable for industrial production.