377 results about "Acid tolerance" patented technology

The invention belongs to the technical field of microorganisms, and particularly relates to a plant lactobacillus B534 strain and application thereof in preparation of functional products beneficial to sleep improvement and emotion regulation. The lactobacillus plantarum B534 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the lactobacillus plantarum B534 is CGMCC No.33031. The lactobacillus plantarum B534 is named as lactobacillus plantarum B534. The plant lactobacillus B534 provided by the invention is a natural strain and has food safety; the lactobacillus plantarum B534 has relatively strong acetylcholin esterase inhibition activity, and also has relatively strong capability of synthesizing and secreting GABA (gamma-aminobutyric acid), 3-HB (3-hydroxybutyric acid) and 5-HT (5-hydroxytryptamine); meanwhile, the plant lactobacillus B534 has relatively strong acid resistance and cholate resistance and also has relatively strong tolerance to simulated gastrointestinal fluid, so that the plant lactobacillus B534 has relatively good intestinal colonization ability and has important application value in the fields of products beneficial to sleep improvement, emotion regulation products and the like.

The invention relates to pediococcus pentosaceus with a cholesterol lowering function and application thereof, and belongs to the technical field of microorganisms. The invention discloses pediococcus pentosaceus with a cholesterol lowering function and application of the pediococcus pentosaceus. The pediococcus pentosaceus R5 (GDMCC No.65659) is separated from special fermented food stinky acid in Guizhou. The in-vitro cholesterol removal rate of the pediococcus pentosaceus R5 can reach 59%, and the pediococcus pentosaceus R5 has the characteristics of acid / cholate resistance, no hemolysis and sensitivity to common antibiotics; an in-vivo lipid-lowering experiment is carried out on a hyperlipidemia model mouse, and the result shows that the strain R5 can remarkably improve the in-vivo dyslipidemia state of the mouse, reduce liver fat accumulation and protect the liver at the same time, and can be used for developing lipid-lowering related probiotic functional products. The strain R5 provided by the invention provides a natural and safe probiotic for preventing and / or treating hyperlipemia.

The invention relates to acid-resistant sulfate reducing bacteria and application thereof, and belongs to the technical field of microorganisms. The acid-resistant sulfate reducing bacterium disclosed by the invention is Desulfovibrio desulfuricans QY411, and is preserved in the Guangdong Microbial Culture Collection Center on March 14, 2024, and the preservation number of the acid-resistant sulfate reducing bacterium is GDMCC No: 64412. The acid-resistant sulfate reducing bacterium provided by the invention can survive in an environment with the pH value of 4, and can be applied to ecological reconstruction of acid tailings and waste dump soil, the pH values of mining wasteland soil and surface water after ecological reconstruction are remarkably increased, the concentration of SO4 < 2-> in the soil is remarkably reduced, the acidification process in the mining wasteland soil is effectively inhibited, and the acid-resistant sulfate reducing bacterium has the advantages of being safe and reliable. Therefore, the acidification process in the mining wasteland is effectively controlled. Meanwhile, soil heavy metal pollution is reduced, and vegetation growth is recovered.

The invention provides a plant lactobacillus YH001 with bacteriostatic activity as well as a screening method and application of the plant lactobacillus YH001. The phytobacterium plantarum YH001 has the taxonomic name of Lactiplantibacillus plantarum, is preserved in the Guangdong Microbial Culture Collection Center, and has the preservation number of GDMCC No: 65693, the preservation number of Lactiplantibacillus plantarum YH001 is CGMCC NO: 65693, and the preservation number is CGMCC NO: 65693. The preservation date is December 30, 2024. The strain has excellent biological characteristics of no hemolysis, acid resistance, cholate resistance and the like, and has wide application potential in the fields of food, medicine and the like. The fermentation supernatant still keeps high bacteriostatic activity after being treated at a high temperature of 121 DEG C, and shows excellent thermal stability. Therefore, the strain has a wide application prospect in research and development of antibacterial agents, food leavening agents and preservative products.

The invention relates to the technical field of biological medicine, and discloses a formula of compound probiotic enzyme powder for promoting gastrointestinal microecological balance and nutrient absorption, by constructing a cross feeding system of bifidobacteria, lactobacillus and butyric acid bacteria and combining a stomach-intestine stage enzyme activity gradient adaptation strategy and a microenvironment response type double-layer embedding technology, the gastrointestinal microecological balance and nutrient absorption can be promoted, and the gastrointestinal microecological balance and nutrient absorption can be promoted. And cross-scale synergy of flora metabolism, substrate conversion and targeted release is realized. Wherein the sodium alginate-pectin composite embedding structure forms a pH buffer micro-area in a gastric acid environment, and proton pump gene expression of the acid-resistant strain is synchronously triggered; the prebiotics compounding and step-by-step enzymolysis process breaks through a closed-loop path of fiber degradation-flora proliferation-short-chain fatty acid generation. Compared with a traditional single-strain intervention scheme, the method has the advantages that double limitations of flora function splitting and process isolation are broken through, and systematic technical advantages are formed in the aspects of improving flora colonization stability, enhancing metabolite conversion efficiency and inhibiting pathogenic bacterium colonization.

The invention discloses a bacillus thuringiensis L3 and an application thereof. The preservation number of the bacillus thuringiensis L3 is CCTCC (China Center for Type Culture Collection) NO: M 2025487. The gene sequence of the 16S rDNA of the bacillus thuringiensis L3 is as shown in SEQ ID NO: 1. Under different adversity stress conditions, the bacillus thuringiensis L3 shows a certain stress resistance potential. Research finds that the bacillus thuringiensis L3 has certain acid resistance, high temperature resistance, cholate resistance and drought resistance, and has strong alkali resistance.

The present invention discloses a Lactobacillus plantarum and its application in the preparation of functional preparations for preventing and treating inflammatory bowel disease, as well as antioxidant, anti-inflammatory and antibacterial functions. It has excellent ability to scavenge reactive oxygen species, as well as good probiotic characteristics such as acid tolerance, bile salt tolerance, hydrophobicity, self-aggregation ability and inhibition of common human pathogenic bacteria. At the same time, it can improve intestinal inflammation, has a preventive and therapeutic effect on inflammatory bowel disease, and has high biological safety. It can be used as a probiotic preparation to relieve inflammatory bowel disease, improve intestinal mucosal damage and colon inflammation, providing a new strategy for the prevention and treatment of inflammatory bowel disease and having potential clinical application prospects.

The invention relates to the technical field of microorganisms, in particular to animal-derived pediococcus acidilactici and application thereof. The pediococcus acidilactici is classified and named as pediococcus acidilactici, the strain number of the pediococcus acidilactici is 12NN1, the pediococcus acidilactici is preserved in Guangdong Microbial Culture Collection Center on January 2, 2025, and the preservation number of the pediococcus acidilactici is GDMCC No: 65706. The strain is resistant to acid and cholate, has a remarkable inhibiting effect on growth and reproduction of pathogenic escherichia coli and staphylococcus aureus, is safe and non-toxic, does not have pathogenicity, and provides beneficial strain resources and biological materials for development of corresponding microbial preparations. Therefore, the compound can be used for preparing a biocontrol preparation for inhibiting the growth of pathogenic bacteria, preparing animal feed and preparing a preparation for preventing, improving and / or treating gastrointestinal tract pathogenic bacteria infection, so that the compound has a wide application prospect.

This application relates to the field of food microbiology, and specifically discloses a Lactobacillus plantarum capable of producing 5-methyltetrahydrofolic acid, its fermentation method and application. A Lactobacillus plantarum inm30-LP capable of producing 5-methyltetrahydrofolic acid, with a preservation date of December 26, 2022, and the preservation unit is the General Microbiology Center of the China Committee for Culture Collection of Microorganisms, and the preservation number is CGMCC No. 26278. This application is based on the milk curd in the Gannan Tibetan area as the food source, isolates probiotics, and screens out Lactobacillus plantarum with high folic acid production from them. Moreover, the strain has obvious advantages over other lactic acid bacteria in terms of acid resistance and bile salt resistance, is suitable for growing in the gastrointestinal environment, has the ability to proliferate, has no antibiotic resistance, has antibacterial activity, and can produce bacteriocin.

The invention relates to the technical field of agriculture, provides a red soil hardening reduction conditioner based on modified straw adsorption carbon as well as a preparation method and application thereof, and solves the problems that an existing red soil hardening conditioner is poor in soil improvement effect and does not have a slow release function. Comprising the following steps: (1) preparing oyster shell-biochar composite powder; (2) loading functional components; (3) directional immobilization of microorganisms; (4) coating a slow-release layer; (5) activating treatment; the red soil hardening reduction conditioner is prepared from the following raw materials in parts by weight: 80 to 100 parts of oyster shell powder, 100 to 200 parts of modified straw adsorption carbon, 10 to 30 parts of rice hull powder, 30 to 50 parts of nano dihydrate gypsum, 100 to 200 parts of a polyglutamic acid solution, 10 to 50 parts of acid-resistant bacillus, 15 to 30 parts of sodium alginate, 30 to 60 parts of humic acid, 10 to 30 parts of carbide slag and 30 to 60 parts of attapulgite.

The invention discloses a porcine epidemic diarrhea virus (PEDV) infection resistant Min pig source lactobacillus salivarius LSM231 and application thereof, and belongs to the technical field of microorganisms. The Min pig source lactobacillus salivarius is named as Ligilbacillus salivarius LSM231 and is preserved in the China Center for Type Culture Collection, the preservation number is CCTCC NO: M 20242330, and the preservation time is October 25, 2024. The Min pig source lactobacillus salivarius has the advantages that the Min pig source lactobacillus salivarius is named as Ligilbacillus salivarius LSM231 and is preserved in the China Center for Type Culture Collection (CCTCC); the screened Min pig source lactobacillus salivarius is symbiotic probiotics in intestinal tracts and is high in safety performance, and experimental results show that the Min pig source lactobacillus salivarius LSM231 has relatively good antibacterial, acid-resistant and cholate-resistant characteristics, has a remarkable PEDV (porcine epidemic diarrhea virus) resisting effect, and can relieve clinical symptoms caused by PEDV infection and promote growth of piglets, so that the Min pig source lactobacillus salivarius LSM231 can be used for preventing and treating the porcine epidemic diarrhea. The compound can replace antibiotics to be used for preparing a micro-ecological preparation for resisting PEDV (porcine epidemic diarrhea virus) infection.

The invention relates to the field of microorganisms, and discloses a Lactococcus lactis subsp. Lactis VB346 which is preserved in the China Center for Type Culture Collection on September 20, 2024, and the preservation number is CCTCC NO: M 20242013. The invention further discloses a preparation method of the Lactococcus lactis subsp. Lactis VB346. The lactococcus lactis subsp. Lactis VB346 disclosed by the invention has the capability of efficiently degrading purine nucleoside, and can decompose purine nucleoside in ingested food in intestinal tracts, so that the absorption amount of purine nucleoside by small intestines is reduced. The polypeptide has the ability of degrading bilirubin, and can be used for treating or improving hyperbilirubinemia, or improving liver and gall injury caused by hyperbilirubin; meanwhile, the acid resistance required by survival in the stomach, the cholate tolerance required by survival in the intestinal tract and the stable survival ability in the intestinal tract are achieved, and the bacillus subtilis has huge application prospects in preparation of drugs, microbial agents, health care products or food.

The invention relates to bacillus subtilis Y32, and the preservation number of the bacillus subtilis Y32 is CGMCC (China General Microbiological Culture Collection Center) No.34819. The strain can produce xylanase with high temperature resistance and high stability, various hydrolases such as beta-mannase, protease and the like, exopolysaccharides and botrytis cinerea inhibition, and is resistant to acid, swine bile salt and 10% NaCl. The optimum temperature of xylanase produced by the strain is 55 DEG C, the optimum pH value is 6.0, under the conditions, the half-life period is 12.5 h, the relative enzyme activity is still kept above 80% after incubation for 3 h, and the xylanase has good thermal stability. The strain can also improve the yield of cucumbers and pinellia ternate, and can also improve the laying rate of 70-week-old hyline brown laying hens from 70.4% to 90.1%. The strain has a good application prospect in the fields of agriculture, food processing and cosmetic production.

The invention belongs to the technical field of microorganisms, and particularly relates to bacillus cereus as well as a culture and application thereof. The preservation number of the bacillus cereus AK-LY1 is CCTCC (China Center For Type Culture Collection) NO: M 2025756. The bacillus cereus AK-LY1 disclosed by the invention is excellent in trypsin resistance, good in cholate resistance, acid resistance and high temperature resistance, good in inhibition performance on antibiotics such as strong penicillin and chloramphenicol, and relatively good in adhesion capacity in intestinal tracts, and provides a relatively good microecological preparation reference basis for expanding a bacillus cereus resource library and applying the bacillus cereus.

The invention relates to the technical field of soil conditioners, in particular to a soil conditioner for soil acidification treatment as well as a preparation method and application of the soil conditioner. Comprising the following components in parts by weight: 20-35 parts of red mud, 10-15 parts of a humic acid-sodium alginate compound, 10-15 parts of zeolite, 5-8 parts of a silicon-calcium-magnesium fertilizer, 4-8 parts of an acid-proof bacterial agent and 10-15 parts of a bio-based adhesive. The acidified soil treatment effect can be better, and the effect is more durable.

This invention belongs to the field of microbial technology, specifically relating to a strain of *Pediococcus pentosaceus* SUN02 with acetylcholinesterase inhibitory activity and its applications. This *Pediococcus pentosaceus* SUN02 is deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 29705. The *Pediococcus pentosaceus* SUN02 provided by this invention exhibits strong acid and bile salt resistance, good tolerance in simulated gastric and intestinal fluids, and can successfully reach the human intestine. *Pediococcus pentosaceus* SUN02, in its bacterial suspension and inactivated cells, possesses strong acetylcholinesterase inhibitory activity, produces γ-aminobutyric acid (GABA), and has antioxidant and other probiotic functions. Its application in probiotic products may help improve Alzheimer's disease, enhance learning and memory abilities, regulate mood, and improve sleep.

The invention provides low-temperature alginate lyase and application thereof, namely alginate lyase with low-temperature catalytic activity, which is separated from marine-derived cold monad bacteria, and the amino acid sequence of the alginate lyase is SEQ ID NO: 1; the amino acid sequence of the alginate lyase after the signal peptide is removed is SEQ ID NO: 3. The algin lyase Alg3889 provided by the invention has the optimal reaction temperature of about 10-16 DEG C, has good temperature stability, and still keeps more than 70% of enzyme activity after being incubated at 0-70 DEG C for 1 hour; the optimum pH value of the strain is 8.0, after the strain is incubated in a buffer system with the pH value of 3.0-10.6 for 12 h, about 90% of the highest enzyme activity can be kept, and the strain has high acid resistance and alkali resistance. The degradation product of the alginate oligosaccharide is alginate oligosaccharide with the polymerization degree of 2-6, and the alginate oligosaccharide has high application value.

The invention belongs to the field of microorganisms, and particularly relates to a salt-resistant and acid-resistant rhodotorula sp. Capable of producing ethyl phenylacetate and application of the rhodotorula sp. In order to develop a new path for the source of ethyl phenylacetate, the invention screens, separates and purifies a strain of salt-resistant and acid-resistant Rhodotorula daliensis S9 capable of producing ethyl phenylacetate from thick broad-bean sauce, and the preservation number is CCTCC NO: M 2025465. The invention also discloses a preparation method of the Rhodotorula daliensis S9 capable of producing ethyl phenylacetate from the thick broad-bean sauce and a preparation method of the Rhodotorula daliensis S9 capable of producing ethyl phenylacetate from the thick broad-bean sauce. The strain has the characteristics of high temperature resistance, acid resistance and salt resistance; and when the strain is used for fermentation culture of thick broad-bean sauce, the yield of ethyl phenylacetate can be increased from 104.86 mu g / Kg to 522.11 mu g / Kg. Therefore, when the rhodotorula sp. S9 is applied to the field of food fermentation, not only can the fermentation flavor of foods such as thick broad-bean sauce, soy sauce and bread be improved, but also a novel method for synthesizing ethyl phenylacetate simply, conveniently, environment-friendly and safely can be provided, and the rhodotorula sp. S9 has a wide prospect in the field of food application.

The invention relates to a cellulose microbacterium J6, the preservation number of which is CCTCC (China Center For Type Culture Collection) NO: M2025107, and the bacterial strain has the capability of degrading cellulose, protein and starch, is acid-resistant, alkali-resistant, salt-resistant and heavy metal ion-resistant, and can grow in a temperature range of 10-50 DEG C. The invention also discloses an application of the strain in aerobic composting, and the strain can effectively promote a compost body to enter a high-temperature stage more quickly, accelerate the composting process, shorten the decomposition period and improve the decomposition degree.

The invention belongs to the technical field of microorganism application, and particularly relates to cat-source bacillus subtilis and application thereof. The strain number of the cat source bacillus subtilis is 11-6Q1, the cat source bacillus subtilis is preserved in the China Center for Type Culture Collection, and the preservation number of the cat source bacillus subtilis is CCTCC NO: M 20241474. The bacillus subtilis strain 11-6Q1 disclosed by the invention is separated from healthy pet cat excrement and has acid-resistant and high-temperature-resistant effects. The bacterium has a strong hydrolysis effect on protein, and can relieve digestion and absorption of protein in intestinal tracts, maintain animal intestinal flora balance, improve animal health and improve animal immunity.

The invention belongs to the field of prevention and control of bacterial diseases of aquatic animals, and discloses an acid-resistant broad-spectrum vibrio parahaemolyticus bacteriophage LVPP58 and application of the acid-resistant broad-spectrum vibrio parahaemolyticus bacteriophage LVPP58 in vibrio disease prevention and control, and the preservation number of the bacteriophage is CCTCC M 2025322. The bacteriophage is a broad-spectrum vibrio parahaemolyticus bacteriophage, not only can split vibrio parahaemolyticus, but also can split vibrio alginolyticus, the bacteriophage LVPP58 is stable in titer at the pH of 3-11 and the temperature of 30-60 DEG C, the bacteriophage and antibiotics are combined for use, a synergistic effect can be generated, the treatment effect is enhanced, the occurrence rate of drug resistance is reduced, and a new strategy is provided for solving the problem of bacterial drug resistance.

The invention discloses pichia kudriavzevii for high yield of L-malic acid as well as a construction method and application thereof, and belongs to the technical field of biology, the pichia kudriavzevii is named as YM-023 and classified as Pichia kudriavzevii, the preservation number is CGMCC No. 35487, the preservation time is July 31, 2025, and the preservation number is CGMCC No. 35487. The preservation unit is China General Microbiological Culture Collection Center (CGMCC). The method comprises the following steps: starting from an acid-resistant yeast Kudriavzevii pichia pastoris strain YL-000 (the preservation number is CGMCC No.35680), carrying out metabolic engineering transformation to obtain a transformed strain, and ensuring that the transformed strain can realize efficient production of L-malic acid in a fermentation mode of adding a small amount of calcium carbonate neutralizer.

The invention discloses enterococcus faecium capable of degrading three mycotoxins at the same time and application of the enterococcus faecium, and belongs to the technical field of microorganisms. The enterococcus faecium JBHBIO-BSC1 disclosed by the invention is preserved in the China Center for Type Culture Collection, and the preservation number of the enterococcus faecium JBHBIO-BSC1 is CCTCC (China Center for Type Culture Collection) NO: M 20241918. The enterococcus faecium disclosed by the invention has the most remarkable characteristic that the enterococcus faecium has an obvious degradation effect on the aflatoxin, the vomitoxin and the zearalenone, and the degradation rates of the aflatoxin, the vomitoxin and the zearalenone can respectively reach 74.67%, 85.83% and 82.12%. The enterococcus faecium disclosed by the invention is natural, safe, acid-resistant, alkali-resistant and high-temperature-resistant, and has relatively strong organic acid production capacity. In addition, the strain has an obvious inhibition effect on escherichia coli, salmonella and staphylococcus aureus, and is expected to be applied to the fields of food processing and microbial pharmacy.

The invention discloses an acid mine pollution treatment method based on microbial regulation and control, and belongs to the technical field of environmental protection engineering, and the acid mine pollution treatment method comprises the following steps: S1, screening and domesticating acid-resistant and heavy metal-resistant functional microbial communities; s2, constructing a sectional bioreactor, and filling the sectional bioreactor with a corresponding composite biological carrier; s3, inoculating the functional microbial community obtained by domestication in the step S1 into the sectional reactor constructed in the step S2, and carrying out biofilm culturing in simulated AMD; and S4, introducing AMD to be treated into the reaction system in the step S3, and regulating and controlling microbial activity through pH gradient regulation and dynamic supply of electron donors to realize heavy metal precipitation and water quality remediation. According to the method, closed-loop treatment of sulfate reduction, heavy metal precipitation and acid neutralization is achieved through the synergistic effect of microbial regulation and control and the functionalized biological carrier, and the problems of efficiency, cost and secondary pollution of traditional AMD treatment are solved.

The invention discloses a preparation method of recombinant catechol-2, 3-dioxygenase and an application of the recombinant catechol-2, 3-dioxygenase. According to the invention, a novel catechol-2, 3-dioxygenase group C23O927 is identified from a constructed metagenome library, and the catechol-2, 3-dioxygenase group C23O927 is a key enzyme for catalyzing degradation of polycyclic aromatic hydrocarbon pollutants. A C23O927-pET32a (+) recombinant expression vector is constructed and converted into host bacteria, heterologous expression of target protein is achieved, and the prepared recombinase belongs to acid enzymes and has good resistance to high temperature, acid, alkali, high-concentration metal ions, organic solvents and surfactants. The recombinase provided by the invention can be used in an acidic environment, has very good enzymatic activity, can catalytically degrade various catechol compounds, can be better used for treating polycyclic aromatic hydrocarbon polluted environments, and has important significance for developing more products which can tolerate various extreme environments and efficiently catalyze degradation of polycyclic aromatic hydrocarbons.

The invention belongs to the technical field of microbiological detection, and discloses a PCR primer combination for detecting mixed microorganisms in a food fermentation process and a detection method thereof. The primer combination comprises a primer pair used for detecting pediococcus pentosaceus, a primer pair used for detecting plant lactobacillus, a primer pair used for detecting lactobacillus mucus fermentation, a primer pair used for detecting bacillus amyloliquefaciens and a primer pair used for detecting lactobacillus acidophilus. The primer pair is at least one of a primer pair for detecting lactobacillus helveticus and a primer pair for detecting acetobacter pasteurianus. The primer combination or the kit containing the primer combination can realize species level specific detection of microorganisms, and can be used for qualitatively / quantitatively detecting food and key functional microorganisms in the food fermentation process, so that the dynamic change of a target flora in the fermentation process is quickly and accurately monitored.

The invention discloses a microorganism, a microbial agent, food, a method for preparing the food and a method for producing pectinase, and relates to the field of microorganisms. The microorganism is plant lactobacillus, and the preservation number of the microorganism is CGMCC (China General Microbiological Culture Collection Center) No.31283. The new strain of the plant lactobacillus obtained through screening has the advantages of being high in growth speed, high in acid production capacity, acid resistance and salt resistance, high in pectinase production capacity, high in nitrite and nitrate metabolism capacity and the like; the new strain is used for developing salt-reduced fermented food, the process adaptability is good, and the prepared fermented food is rich in lactic acid bacteria content, nutritional, healthy and unique in flavor; a food fermentation process developed by utilizing the new strain is high in fermentation efficiency, short in production period and high in application value.

The invention discloses a preparation method and application of a zero-emission salt-resistant and acid-resistant hydrogel antibacterial filtering membrane. The method comprises the following steps: preparing a calcium alginate hydrogel membrane through calcium ion cross-linking, filtering a cross-linked cross-linking agent aqueous solution in which the concentration of calcium ions is reduced and sodium ions are introduced by using a nanofiltration membrane, supplementing soluble calcium salt into the ionic cross-linking agent aqueous solution in which the sodium ions are removed, and recovering the mass percent concentration of the calcium ions to be 2.5%, so that the regeneration of the ionic cross-linking agent solution is realized. And carrying out chemical crosslinking on the calcium alginate hydrogel membrane by adopting glutaraldehyde to obtain the zero-emission salt-resistant acid-resistant hydrogel antibacterial filtering membrane. Glutaraldehyde is added into the aqueous solution of glutaraldehyde after chemical crosslinking, and the mass percent concentration of glutaraldehyde is recovered to 3.0%, so that the regeneration of the chemical crosslinking agent solution is realized, and the zero emission requirement is met. The excellent antibacterial property of glutaraldehyde endows the film with obvious advantages in the aspects of storage and bacterial breeding inhibition. The hydrogel antibacterial filtering membrane can be applied to separation of dyes and inorganic salts under the conditions of high salt content and acidic pH.

The invention relates to the field of microorganisms, in particular to kitten source pediococcus pentosaceus JZ01 and application thereof. The kitten source pediococcus pentosaceus JZ01 provided by the invention is preserved in the China General Microbiological Culture Collection Center (CGMCC) on June 19, 2024, and the preservation number is CGMCC NO.31002. The kitten source pediococcus pentosaceus JZ01 has the advantages that the kitten source pediococcus pentosaceus JZ01 is The kitten source pediococcus pentosaceus JZ01 disclosed by the invention has relatively strong acid resistance, bile salt resistance and extremely strong intestinal adhesion capacity, has a good inhibition effect on common pathogenic bacteria, is sensitive to various antibiotics and has good probiotic potential, a new candidate strain is provided for pet food, health-care treatment products or probiotic preparations, and the kitten source pediococcus pentosaceus JZ01 has a good application prospect. Wide application prospects are realized in the aspects of preparing pet foods, health care products, medicines and the like.

The invention discloses a selenium-rich microecological preparation. The selenium-rich microecological preparation is prepared by the following steps: S1, activating strains; s2, preparing a selenomethionine premixed solution; s3, co-culture of bacteria and selenium; s4, forming a selenoprotein complex; s5, preparing a coating material; s6, coating with an emulsion; s7, acid-resistant layer coating; and S8, enteric activation treatment. According to different growth stages of the meat geese in the antibiotic-free breeding, a daily ration formula is directionally intervened: in the brooding period of the meat geese, the selenium-rich microecological preparation and daily ration feed are mixed in a dry mode, and conventional feeding is carried out; in the growth period of the meat geese, acidifying the selenium-rich microecological preparation, preparing the selenium-rich microecological preparation into a solution, and feeding the meat geese with the solution in the form of drinking water; in the fattening period of the meat geese, the premix containing the selenium-enriched microecological preparation is mixed with daily ration, and the meat geese are fed in an intensified mode. According to the invention, selenium-rich breeding of meat geese can be realized, economic benefits are increased, antibiotics can be replaced, and the eating safety and edible value of meat geese are improved.