1078 results about "Rapeseed" patented technology

The invention provides a grain impurity rate and breakage rate monitoring system and method for a combine harvester. The grain impurity rate and breakage rate monitoring system comprises a grain sampling device, an image acquisition device and a control device, the grain sampling device is installed on the side wall of the vertical grain conveying barrel, a grain inlet of the grain sampling device is connected with a grain outlet hole in the side wall of the vertical grain conveying barrel through a hose, and a grain outlet of the grain sampling device is connected with a feeding port of the image acquisition device through a connecting pipe; the image acquisition device is connected with the control device, an image acquisition module of the control device controls the image acquisition device to acquire a grain image, and online calculation of the impurity rate and the breakage rate of the grain is realized on the grain image through a YOLOv8-Seg instance segmentation model of the image processing module in combination with an impurity rate and breakage rate mathematical model; finally, a detection result is displayed on a display screen in real time, visual numerical values can be provided for an operator of the combine harvester, and the operator is helped to adjust working parameters of the harvester. The method can be used for harvesting rice, wheat, oilseed rape and soybeans, and has wide applicability.

The invention discloses an application of a rape BnC05ERF378 gene in improving the waterlogging resistance of a plant. The nucleotide sequence of the rape BnC05ERF378 gene is as shown in SEQ ID NO. 1. According to the invention, a brand new stain-resistant gene BnC05ERF378 is cloned from rape for the first time, and is transferred into arabidopsis thaliana to construct a pure line plant and complete stain resistance identification. Experimental results show that the growth state of the transgenic BnC05ERF378 gene arabidopsis thaliana is remarkably superior to that of a wild type after waterlogging treatment, the transgenic BnC05ERF378 gene arabidopsis thaliana can normally bolt, the survival rate is increased from 12.5% to 37.5%, it is fully proved that the gene can enhance the survivability of the plant in a waterlogging environment by regulating the response mechanism of the plant to waterlogging stress, and the survival rate of the plant is increased from 12.5% to 37.5%. And a key guarantee is provided for stable growth of plants under high-humidity, waterflooding and other adverse conditions. According to the discovery and application of the waterlogging-resistant gene BnC05ERF378 disclosed by the invention, a clear functional gene target is provided for improvement of waterlogging resistance of crops. By constructing an expression vector containing the gene and combining mature technologies such as agrobacterium transformation, the gene can be stably introduced into a target plant, and a pure transgenic plant is obtained.

The invention relates to the technical field of production of organic chili oil bean halves, and discloses an organic chili oil bean halve production process which comprises the following steps: cooking and curing organic broad beans, cooling to make koji, and carrying out heat preservation fermentation to obtain sweet halves; mixing and pickling the organic fresh chilies with sodium chloride to obtain low-salt chilli embryos; mixing the sweet petals with the low-salt chili embryos, and adding an emulsifying active medium to obtain soy sauce mash; performing staged temperature-controlled fermentation and normal-temperature solarization curing on the soy sauce mash; and grinding and sterilizing the fermented and cured material to obtain a finished product of the organic chili oil bean halves. The preparation method comprises the following steps: pre-dispersing rapeseed oil into fine liquid drops by using active yellow mustard micro powder; the calcium carbonate buffering agent releases calcium ions in response to the acidity change of the system, pectin in chili is induced to be subjected to a cross-linking reaction, a three-dimensional gel network is constructed in soy sauce mash, dispersed oil drops are physically embedded, the oil drop merging and migration resistance is increased, and the oil separation rate of the product in the storage process is reduced.

The invention discloses a rape population canopy photosynthetic measurement method and device and a storage medium, and relates to the technical field of plant image processing. The method comprises the following steps: constructing a rape group point cloud data set with organ semantic information; training a rape group organ point cloud segmentation model by using the point cloud data set; segmenting the rape group organ point cloud by using the trained point cloud segmentation model; on the basis of the point cloud data of the rape group after organ segmentation in the whole growth period, rape group structure indexes are extracted; and constructing a photosynthetic capacity measurement index based on the rape population structure index, and obtaining photosynthetic capacity estimation of the whole-growth-period rape population. According to the method, high-throughput measurement of the photosynthetic parameters of the rape population can be accurately realized.

The invention relates to the technical field of plant polysaccharide extraction, in particular to a process for synergistically extracting rape pollen polysaccharide by a compound enzyme, which comprises the following steps: crushing rape pollen to 200-300 meshes by pulse airflow, performing microwave-ultrasonic degreasing by using a 85% ethanol solution, mixing with an acetic acid-sodium acetate buffer solution with the pH value of 4.8, and performing ultrasonic extraction to obtain a crude extract; the method comprises the following steps: adding a compound enzyme system which is immobilized by Fe3O4 (at) SiO2 nanoparticles by cellulase, pectinase, protease and beta-glucosidase according to a mass ratio of 2: 1: 1: 0.5, carrying out enzymolysis for 4 hours in a 50 DEG C constant-temperature water bath and a 15V / cm pulsed electric field, carrying out enzyme deactivation on enzymatic hydrolysate, carrying out gradient ethanol precipitation, carrying out ultrafiltration deproteinization, and carrying out vacuum pulsation freeze drying to obtain the polysaccharide. According to the method disclosed by the invention, the cell wall breakage rate and the enzymolysis efficiency are improved through pulsed airflow crushing, magnetic nano immobilized enzyme and pulsed electric field assistance, the polysaccharide yield reaches 7.32%, the purity is 91%, the beta-glucosidic bond proportion and the antioxidant activity are remarkably improved, the enzyme can be recycled and reused, the drying time is shortened, and efficient and low-consumption extraction is realized.

The invention relates to the technical field of agricultural biological information, in particular to a rape phenotype-genotype correlation analysis system based on deep learning, which comprises a phenotype image acquisition module, an image preprocessing module, an image phenotype feature extraction module, a multi-dimensional phenotype integration module, a deep learning model training module and a correlation analysis module, the image phenotypic feature extraction module creatively fuses a differential geometry theory and a deep learning technology, maps rape phenotypic features from an Euclidean space to a Riemannian manifold space through a multi-scale differential manifold representation network, and calculates feature correlation based on a geodesic distance by using a manifold attention weighting mechanism. The multi-scale geodesic integration module is adopted to realize organic fusion of phenotypic characteristics of different scales, the association analysis module adopts a multi-level linear regression model to analyze key agronomic characters of the rape, an association relationship between phenotypes and genotypes is established, and the phenotypic characteristic extraction accuracy under a complex background is improved by 35.2% compared with that of a traditional method.

The invention discloses application of a transcription factor BnaMYB52 in regulation and control of drought resistance of rape, and relates to the technical field of biology. The amino acid sequence of the transcription factor BnaMYB52 is as shown in any one of SEQ ID NO. 5 to 8. According to the invention, the negative regulation effect of the transcription factor BnaMYB52 in the drought resistance of the rape is disclosed for the first time, and a brand new molecular target and a genetic improvement strategy are provided for improving the drought resistance of the rape. Through in-depth study, it is found that knockout of the BnaMYB52 gene can significantly enhance the tolerance of rape plants to the drought environment. In addition, a new drought-resistant rape germplasm obtained after genetic modification of the gene shows excellent adaptive capacity in a drought stress environment. The technical breakthrough not only helps to solve practical problems faced by rape planting in arid regions, but also lays a solid theoretical foundation and technical support for cultivating high-yield and high-quality rape varieties adapting to extreme climate conditions.

The invention relates to the technical field of preparation methods of giant salamander peptide and eucommia ulmoides male flower tablets, in particular to a preparation method of giant salamander peptide and eucommia ulmoides male flower tablets. The giant salamander peptide and eucommia ulmoides male flower tablets comprise the following raw materials in parts by weight: 30-40 parts of giant salamander peptide powder, 20-25 parts of eucommia ulmoides male flowers, 10-15 parts of rape pollen, 10-15 parts of sea cucumber intestine peptide powder, 10-12 parts of a filler, 3-5 parts of an adhesive and 1-3 parts of a lubricant. According to the invention, the eucommia male flower contains geniposidic acid, chlorogenic acid, aucubin lignin, iridoid and phenylpropanoid natural active nutrient substances, flavonoid compounds, polysaccharide and other bioactive substances in the rape pollen, polysaccharide and peptide components in the sea cucumber intestine peptide powder, and the like. The andrias davidianus peptide powder is added into the andrias davidianus peptide powder, the andrias davidianus peptide powder cooperates with antioxidant peptide and immunomodulatory peptide in the andrias davidianus peptide powder to jointly form a rich bioactive substance library, and the components regulate the physiological functions of the human body from different angles, provide comprehensive nutrition support for the body and improve the fatigue resistance of the human body.

The invention discloses a portable rapeseed quality detection deep learning model lightweight method, which comprises the following steps: constructing a teacher model and a student model, and extracting local wave crest features and global time domain features from near infrared spectrum data of rapeseeds by using the teacher model and the student model, the teacher model and the student model output predicted values of the oil content of the rapeseeds; according to the difference between the predicted value output by the student model and the true value, the difference between the predicted values output by the teacher model and the student model, the difference between the local wave crest feature and the global time domain feature extracted by the teacher model and the student model, and the difference between the predicted values of each wave band of the teacher model and the student model; constructing combined distillation loss; and migrating knowledge of the teacher model to the student model through combined distillation loss, and predicting the oil content of the rapeseeds by using the student model after knowledge migration. And through multi-scale characteristic distillation and structured migration pruning, the operation efficiency of the model is remarkably improved on the premise of ensuring the prediction precision.

The invention belongs to the technical field of plant molecular markers, and particularly discloses an Indel molecular marker related to the salt tolerance of brassica napus and application of the Indel molecular marker. The marker is located at the position of an A01 chromosome scafoldA0133077947 of a brassica napus reference genome ZS11.v10, and the genotype of a polymorphic site is CTAA or C. The invention further discloses a method for identifying the marker of the brassica napus. Large-scale group phenotypic association analysis proves that the molecular marker is remarkably associated with the salt tolerance of the brassica napus in the germination period, and when the polymorphic site genotype is CTAA, the salt tolerance in the germination period is high; when the genotype of the polymorphic site is C, low salt tolerance in the germination period is shown. The invention finds that the polymorphic site is obviously associated with the salt tolerance of the brassica napus for the first time, provides a powerful molecular tool for solving the problems of difficulty in phenotype identification, long period and low efficiency in salt tolerance breeding of the brassica napus, can accurately screen salt-tolerant materials in the early stage, and greatly accelerates the breeding process of new varieties of the salt-tolerant brassica napus.

The invention relates to a heterocyclic pyrimidinedione compound and application thereof in preparation of pesticides and medicines, in particular to the heterocyclic pyrimidinedione compound and application thereof in preparation of herbicides and plant virus resisting agents, and application in preparation of medicines for resisting fungal infection, especially candida auricula infection. The compound provided by the invention has good inhibition effect on KARI enzymes from different sources. Under the dosage of 100g / mu, the weeding effect of the compound provided by the invention on rape during stem and leaf treatment can reach 100%. The compound disclosed by the invention has a good inhibition effect on a candida auricula 12766 strain, and the minimum inhibition concentration (MIC) value of the compound can reach 0.31 mg / L when a YNB culture medium is used. The in-vivo passivation effect of the compound on the tobacco mosaic virus TMV under the concentration of 500 mg / L can reach 64.2%.

The invention discloses an oilseed rape pot seedling transplanter, and relates to the technical field of oilseed rape planting. Comprising a power headstock, a seedling planting mechanism, a support, a transmission shaft and first side plates, the support is connected with the power headstock, the transmission shaft is in transmission connection with the power headstock, the transmission shaft is rotationally connected with the support, the two ends of the transmission shaft are each provided with one first side plate, and a plurality of rotating shafts are arranged between the two first side plates in parallel; each rotating shaft is provided with a plurality of duckbilled planters, and each duckbilled planter is provided with a switch assembly used for controlling the duckbilled planter to be opened and closed, a seedling feeding mechanism and a seedling taking mechanism. According to the rape pot seedling transplanter provided by the invention, automatic transplanting of rape pot seedlings can be realized, the transplanting efficiency of the rape pot seedlings is improved, and labor is saved.

The invention discloses application of oilseed rape chitinase EP3 and a coding gene thereof in improving clubroot resistance of cruciferous plants, and belongs to the technical field of molecular biology. The invention discloses the application of the rape chitinase EP3 in improving the clubroot resistance of plants for the first time. The amino acid sequence of the rape chitinase EP3 is as shown in SEQ ID NO.2; the nucleotide sequence of the coding gene EP3 is shown as SEQ ID NO. 1. Experimental results show that by preparing agrobacterium containing an EP3 gene overexpression vector and using a treating fluid containing the agrobacterium to perform seed soaking and root irrigation treatment, the EP3 gene is overexpressed in a rape plant, and the clubroot resistance of the rape can be remarkably improved. The invention provides a new gene resource and a safe, efficient, simple, convenient and universal prevention and treatment method for preventing and treating clubroot of cruciferous plants, and has remarkable agricultural application value and wide application prospect.

The utility model discloses a rapeseed stir-frying machine, which relates to the technical field of oil product processing and comprises a base, seed stir-frying components are fixedly mounted at the left and right parts of the upper end of the base, and a feed filtering component is fixedly mounted at the right part of the top of each seed stir-frying component. According to the rapeseed frying machine disclosed by the utility model, rapeseeds in the heating furnace can be turned and fried through the seed frying assembly, and can be conveyed to the left part of the inner cavity of the heating furnace during frying. Rapeseeds entering the heating furnace can be filtered and screened in advance through the feeding filtering assembly, and impurities in the rapeseeds are separated and collected. Rapeseeds discharged by the heating furnace can be guided onto the electric conveying belt through the guide plate by the conveying air-cooling assembly, so that the rapeseeds can be subjected to air cooling in the tiled conveying process, and the temperature of the fried rapeseeds is rapidly reduced by a plurality of air spraying heads, so that the oxidation reaction can be inhibited, and the damage of high temperature to nutritional ingredients can be reduced.

The invention discloses a preparation method of a low-protein biological fermentation feed for crayfish, and belongs to the technical field of aquaculture feeds, and the preparation method comprises the following steps: high-temperature fermentation: uniformly mixing soybean meal, rapeseed meal, cottonseed meal and a mixed bacteria solution containing bacillus subtilis, lactobacillus plantarum, saccharomycetes and EM bacteria, and performing high-temperature fermentation to obtain a fermentation product; and performing low-temperature fermentation: adding shrimp shell meal, corn flour, rice bran, cellulase and phytase into the fermentation product, performing uniform stirring to obtain a mixture, and performing low-temperature fermentation on the mixture to obtain the product. According to the low-protein biological fermentation feed disclosed by the invention, by-products such as shrimp shell meal, rice bran and rapeseed meal are used as raw materials of the biological fermentation feed, so that the cost of the feed can be reduced; and the aims of enhancing the nutritional quality, improving the immunity and oxidation resistance of the organism and improving the environment of a culture water body can be fulfilled for culturing the crayfish with the specification of about 12 grams per crayfish.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to application of a brassica napus BnaCP15A gene coded protein to improvement of plant salt tolerance. The technical problem to be solved by the invention is to provide a new choice for improving the salt tolerance of plants. The technical scheme of the invention is the application of the protein coded by the brassica napus BnaCP15A gene in improving the salt tolerance of the plant, wherein the amino acid sequence of the protein is shown as SEQ ID NO.2. The invention further discloses the application of the protein coded by the brassica napus BnaCP15A gene. The salt tolerance of the plant is improved by increasing the expression of the brassica napus BnaCP15A gene in the plant, and the nucleotide sequence of the BnaCP15A gene is as shown in SEQ ID NO. 1. The main purpose of the invention is to provide the application of the protein coded by the brassica napus BnaCP15A gene in improving the salt tolerance of the plant, a new choice is provided for improving the salt tolerance of the brassica napus, and the brassica napus BnaCP15A gene has important application value.

The invention discloses an SNP (Single Nucleotide Polymorphism) marker for auxiliary selection of the leaf number of brassica napus, a primer group, a detection method and application, and relates to the technical field of biology and rape breeding. The SNP marker is located at the 9283404 bp position of a brassica napus A02 chromosome, a basic group of the site is a T allele or a C allele, and the T allele is associated with a leafy character. The SNP marker provided by the invention can solve the technical problem that the breeding efficiency of the high-photosynthetic-efficiency ideal-plant-type rape variety is restricted due to the fact that an effective molecular marker closely linked with the leaf number character of the brassica napus is lacked in the prior art and the character cannot be quickly and accurately screened in the early breeding stage.

The invention relates to bacillus velezensis and protease as well as application of the bacillus velezensis and the protease in meal fermentation. The name of the strain is bacillus velezensis GLY6-6, the preservation number is CGMCC No.33263, the strain is preserved in China General Microbiological Culture Collection Center (CGMCC) on December 31, 2024, and the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No. 3, Beichen West Road, Chaoyang District, Beijing, China. The bacillus velezensis provided by the invention can be applied to meal fermentation, is particularly suitable for fermentation of soybean meal and rapeseed meal, and has the advantages of high enzyme yield, high enzyme activity, good fermentation effect and the like.

The invention discloses a bumblebee candida for efficiently producing sophorolipid as well as a construction method and application of the bumblebee candida. According to the invention, metabolic transformation is carried out on a wild type strain of candida bumblebee, a peroxisome membrane transporter gene PXA1 is knocked out, so that a beta-oxidation pathway of fatty acid competing with a sophorolipid synthesis pathway is moderately inhibited, and on the basis, an endogenous potent promoter pENO is utilized to enhance expression of CYP52M1; therefore, the bumblebee candida engineering bacteria for efficiently producing sophorolipid are constructed. Compared with an original strain, the recombinant candida bumblebee has the advantages that efficient conversion of a fermentation substrate and high yield of sophorolipid can be realized, and the sophorolipid yield of shake flask fermentation reaches 88.50 g / L. The strain is fermented in a 5L fermentation tank and fermented for 7 days after rapeseed oil is added, 232.27 g / L of crude sophorolipid is produced, and high productivity is kept.

The invention relates to the technical field of pomacea canaliculata trapping, killing and preventing, in particular to an efficient pomacea canaliculata trapping and killing method which comprises the following steps: pre-treating drug particles, specifically preparing drug particles for storing trapping and killing drugs, thickening duckweed dry powder, specifically, coating the drug particles with a duckweed dry powder coating with the thickness of 1mm to obtain inner sphere particles, the mikania micrantha ethanol dry powder thickening treatment means that the mikania micrantha ethanol dry powder tightly and uniformly covers the surface of the duckweed dry powder coating by adopting a spraying process on the outer layer of the inner sphere particles. According to the method, the pomacea canaliculata is actively induced to gather and feed by utilizing the rape straw pith core, and aquatic / terrestrial plant wastes are converted into a biological carrier with gradient inducing-poisoning functions; natural toxic components of mikania micrantha are used for replacing traditional chemical pesticides; the straw pith core is used as a universal skeleton, the duckweed dry powder plays the functions of ecological attraction and nutrition supplement, the mikania micrantha ethanol dry powder provides efficient poisoning ability, and the method is especially suitable for green prevention and control of ampullaria gigas in rice cropping areas of the Yangtze River basin.

The invention relates to the technical field of rapeseed oil processing, and discloses a rapeseed oil squeezing and leaching process integrating gradient temperature control and dynamic regulation, which comprises the following steps: S1, receiving and metering raw materials; s2, cooperatively softening, drying and squeezing; s3, dynamic regulation and control conveying and buffering of the rape seed cakes; s4, gradient temperature control leaching and waste heat recovery; s5, optimizing operation of a solvent recovery system; s6, collecting crude oil; drying and softening process parameters are dynamically regulated and controlled in the reverse direction with the squeezing requirement as the target, and precise cooperation of moisture and temperature of the blank pieces is achieved; in the leaching stage, gradient heating evaporation is adopted, and evaporation-removed waste heat is recycled for material preheating, so that the energy consumption is reduced while the solvent separation efficiency is improved; a dynamic matching mechanism based on real-time data and buffer storage is established, the production rhythm of a pre-squeezing workshop and a leaching workshop is coordinated, the problem of capacity imbalance is solved, and the purposes of improving the oil yield and the quality of crude oil, reducing energy consumption and solvent loss and enhancing the continuity and stability of whole-process production are achieved.

This invention relates to the field of agricultural biotechnology, specifically to... BnaC04.bZIP16 Application of genes in improving plant salt tolerance. This invention provides... BnaC04.bZIP16 The gene, whose sequence is shown in SEQ ID NO.1, and whose encoded protein sequence is shown in SEQ ID NO.2, is found in this invention to be highly expressed in wild-type Brassica napus after irrigation with salt water. BnaC04.bZIP16 Genes, and transgenic rapeseed overexpressing this gene, can grow better under salt stress conditions; knockout gene... BnaC04.bZIP16 The genetically modified rapeseed is more difficult to grow in salt-stressed environments, thus verifying... BnaC04.bZIP16 This gene plays a positive regulatory role in enhancing the salt tolerance of rapeseed and provides a key molecular tool for developing new rapeseed varieties with high salt tolerance. This gene has broad application potential and commercial value in the field of agricultural planting, especially in improving the salt tolerance of crops and breeding highly salt-tolerant transgenic crops.

The invention relates to the field of agricultural bioengineering, in particular to application of an oilseed rape BnaA08G0167600WE gene in enhancing pod shattering resistance of oilseed rape. According to the invention, after a transgenic plant is constructed in oilseed rape by utilizing a modern biotechnological means and the oilseed rape gene BnaA08G0167600WE is knocked out, the silique of the mutant is not easy to crack compared with a wild type under the action of external force, and a separation layer and a wood layer of the cross section of the silique of the mutant have more cell connections compared with the wild type, so that the yield of the mutant is improved. And the generation of the mutants provides an important genetic material for further researching the function of the gene and the effect of the gene in the rape.

The invention relates to the field of microbial technology and ecological restoration of saline-alkali soil, in particular to plant growth-promoting paenibacillus for saline-alkali soil and application of the plant growth-promoting paenibacillus. The microbial inoculum comprises paenibacillus sp. XJ-1, P.megatherium Y-39, Endophytic glutamicum G-30 and Enterobacter C-29, and is prepared by mixing the paenibacillus sp. XJ-1, the P.megatherium Y-39, the Endophytic glutamicum G-30 and the Enterobacter C-29 according to a ratio of 1: 1: 1: 1: 1 and carrying out co-culture domestication, function synergistic induction and adaptability synchronous reinforcement. The viable count of the microbial agent is larger than or equal to 1 * 10 cfu / mL, the microbial agent can tolerate 15% NaCl, the pH value of the microbial agent is 9.5, the pH value of soil can be remarkably reduced through root irrigation or seed dressing application, the soil enzyme activity and the crop biomass can be improved, and the microbial agent is suitable for saline-alkali soil crops such as oilseed rape and wheat, has the advantages of being high in stress tolerance, synergistic in function, safe in ecology and the like and has wide application prospects.

The invention belongs to the technical field of feed preparation, and particularly relates to a method for preparing a feed raw material through synergistic fermentation of rapeseed dregs and pomace. The method systematically solves the technical problems of anti-nutritional factor residue, pomace decay risk, low fiber utilization rate, unbalanced nutrition and the like of rapeseed meal, and has the core advantages of thoroughness and safety of detoxification, no chemical residue, significantly improved fiber degradation rate, and significantly improved process stability by inhibiting infectious microbe pollution through segmented temperature control. The method provides an industrial feasible path for high-value utilization of the rapeseed dregs and the pomace, and has both economic benefits and environmental benefits.

The invention discloses an anti-hardening protein bar suitable for cold conditions and a preparation method thereof, and belongs to the technical field of food processing. In order to solve the problems of texture hardening and mouthfeel deterioration caused by aggregation and crosslinking of protein molecules in the existing protein bar under a cold condition, the invention provides a method for organically combining specific plant proteins (soybean protein, quinoa protein, winter rapeseed protein and pea protein) rich in antifreeze peptide with antifreeze polysaccharide; the protein bar which can effectively resist hardening and keep good texture and nutritional characteristics in a cold environment is prepared through the processes of extrusion puffing, precise mixing, forming and the like, so that the application of the product in the fields of exercise and fitness, outdoor exploration, emergency storage and the like is expanded, and the market competitiveness of the protein bar is enhanced.

The invention discloses a weed pathogenic bacterium spiropolyspora roseum JZ-28 and application thereof in weed prevention and removal, and belongs to the technical field of microorganisms. The weed pathogenic bacterium, namely the clonostachys rosea JZ-28, is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.41916, and the preservation date is April 15, 2025. The invention further discloses a preparation method of the clonostachys rosea. According to the invention, a weed pathogenic bacterium, namely the spiropolyspora pink JZ-28, is obtained through separation. Through identification, the strain has high pathogenicity to four weeds including chenopodium album, elsholtzia densa, malva verticillata and senecio scandens, the incidence rate of living weed plants reaches 70% or above, and the strain is safe to corn, oilseed rape, cucumber, tomato, eggplant, broad bean and pea, has the potential of being developed into a microbial herbicide for broad-leaf weeds, and is wide in application prospect.

The invention discloses a method for preparing live pig feed through mixed solid-state fermentation of feed rape and rapeseed meal. The method specifically comprises the following steps: harvesting feed rape from a full-bloom stage to a final bloom stage, adding rapeseed dregs, and uniformly mixing to obtain a fermentation substrate with the water content of 55-65%; spraying the compound enzyme bacterium preparation on the fermentation substrate, uniformly stirring, finally compacting by using a packer, sealing, packing and storing, and fermenting for 45 days; the compound enzyme bacterial preparation comprises a bacterial agent and an enzyme agent; the fungicide comprises lactobacillus plantarum, bacillus subtilis, saccharomyces cerevisiae and aspergillus niger; the enzyme agent is prepared from phytase, cellulase, hemicellulase, pectinase, tannase, xylanase and alpha-galactosidase. The feed rape and the rapeseed meal are subjected to mixed solid fermentation, so that the content of phytic acid, glucosinolate and crude fiber is greatly reduced, the content of crude protein is increased, and the nutritional quality of the feed is remarkably improved. The fermented feed is used for replacing part of corn, soybean meal and wheat bran, and the use amount of concentrated feed in live pig breeding is reduced.

The invention relates to an oil preparation method based on an oil material cell reactor principle, which comprises the following steps: S1, comprehensively grading rapeseeds into mature grains and immature grains according to appearance color and grain size; s2, performing after-ripening reaction on the sorted immature rapeseed grains under the conditions of natural illumination or artificial illumination or both of the natural illumination and the artificial illumination and certain temperature and humidity; s3, merging the ripe rapeseeds sorted in the step S1 and the rapeseeds subjected to light-promoted after-ripening treatment in the step S2, and putting the merged rapeseeds in a microwave field or an infrared field or a multi-physical field of the microwave field and the infrared field for collaborative pretreatment so as to continue cell reaction; s4, the rapeseeds subjected to multi-field collaborative pretreatment are subjected to tempering and then guided into a low-temperature oil press to be squeezed to prepare oil; and S5, cooling the squeezed crude oil, and then carrying out precision filtration at a relatively low temperature to obtain finished oil. According to the method, migration of natural nutritional ingredients into oil and generation of a large amount of pleasant aroma substances are promoted at a relatively low temperature, and high-quality edible oil is produced.

The invention relates to the technical field of plants, in particular to application of surfactin in promotion of rape root development. According to the invention, a new function of the surfactin is found, and the surfactin can be used as a signal molecule to promote expression of related genes of auxin of the rape and promote development of lateral roots of the rape and formation of a branch prosite, so that development of a root system of the rape is remarkably promoted. Surfactin can be used for preparing products for promoting root development of rape, such as plant growth regulators, fertilizers or fertilizer additives and the like, and has a wide application prospect.