The invention provides a method for efficiently preparing active polypeptide by fermenting
rapeseed dregs, which comprises the following steps: pretreatment: crushing
rapeseed dregs, adding 3-5% of
reducing sugar, humidifying and sterilizing; performing mixed
bacteria segmented
fermentation: inoculating a composite strain consisting of
bacillus licheniformis SWJS33 with high yield of
aminopeptidase,
aspergillus oryzae and
lactobacillus plantarum, and fermenting at 30 DEG C or 37 DEG C for 72-84 hours; ultrasonic wall breaking: adding water after
fermentation, uniformly mixing, carrying out ultrasonic treatment for 20-30 minutes under the power of 120-200W, and breaking thalli to release
intracellular enzyme; enzymolysis: carrying out enzymolysis for 4-6 hours under the condition of 50-60 DEG C; post-treatment:
enzyme deactivation,
centrifugation and
drying are carried out after enzymolysis, and a polypeptide product is obtained. By adding the exogenous
reducing sugar,
nutrition is enhanced, and
thallus proliferation is accelerated;
bacillus licheniformis SWJS33 with high yield of
aminopeptidase is inoculated, so that the generation of small-molecule active
peptide is further promoted; a mixed
bacteria composite
system and a segmented
fermentation technology are adopted, and
bacteria cells are crushed in an ultrasonic-assisted manner, so that the enzymolysis efficiency is enhanced, and the yield and activity of the polypeptide are remarkably improved.