1366 results about "Rapeseed" patented technology

The invention relates to the technical field of seed processing analysis, in particular to a big data-based rape seed quality evaluation model, which is characterized in that a data pool is constructed by converging multi-source heterogeneous rape data, and high-quality input is provided for subsequent cross-domain feature association; a cross-domain feature interaction graph is constructed, a graph neural network is used for learning, cross-domain feature interaction is visually presented, and a gene-environment interaction relationship is analyzed; through a four-branch multi-head and cross-modal attention analysis atlas, focusing key features, fusing optimization information and generating a high-quality feature vector, high-order feature nonlinear fusion is realized, and the characterization capability of the model on multi-source heterogeneous data is remarkably improved; an evaluation model is constructed through decoding vectors, and high-yield variety data is extracted, so that efficient screening of rape varieties is facilitated; by deploying the generative adversarial network and transferring the learning adaptive model, the evaluation capability is quickly expanded to a new variety, and breeding intelligence is promoted.

The invention discloses a self-adaptive optimization control system and method for rapeseed oil squeezing, and the method comprises the steps: obtaining rapeseed oil squeezing historical data, constructing a rapeseed oil squeezing reference library and a rapeseed oil squeezing prediction model, matching the rapeseed oil squeezing reference library to obtain an initial operation technology of an oil press, and carrying out the rapeseed oil squeezing to obtain an actual oil output index; obtaining a first predicted oil output index, obtaining an oil press bearing wear rate and an oil press state index according to the oil press operation current and the oil press operation state, correcting the first predicted oil output index to obtain a second predicted oil output index, and calculating an oil output index deviation; and selecting a process optimization strategy according to the oil outlet index deviation and the oil press bearing wear rate, and obtaining the optimized operation process of the oil press according to the process optimization strategy. The method not only can improve the efficiency and accuracy of rapeseed oil squeezing optimization control, but also has good interpretability, and can be directly applied to a rapeseed oil squeezing self-adaptive optimization control system.

According to the unmanned aerial vehicle multi-source quantitative remote sensing rape growth state evaluation method, rape is divided into a seedling stage, a flowering stage and a legume stage according to growth nodes, ground hyperspectral and multi-spectral data are synchronously obtained, resolution is increased and decreased, the data are converted into all-dimensional, multi-scale and multi-temporal data, a growth state factor inversion model of the rape is established, and the rape growth state is evaluated. Evaluating the contribution of different growth state factors to the growth vigor of the rape, and evaluating the growth state of the rape based on comprehensive evaluation indexes of leaf area index, overground biomass and chlorophyll content. The method comprises the following steps: establishing a model based on multi-layer linear regression and partial least square optimization models of three types of growth factors LAI, AGB and CC, multi-mode prediction based on entropy aggregation and hierarchical analysis of entropy loading expert knowledge in combination with expert knowledge and agronomic knowledge, and setting a model with an optimal verification index in different growth periods as a growth state evaluation model in the period. The rape growth state evaluation period is short, the pertinence is strong, and the accuracy is good.

The invention provides a grain impurity rate and breakage rate monitoring system and method for a combine harvester. The grain impurity rate and breakage rate monitoring system comprises a grain sampling device, an image acquisition device and a control device, the grain sampling device is installed on the side wall of the vertical grain conveying barrel, a grain inlet of the grain sampling device is connected with a grain outlet hole in the side wall of the vertical grain conveying barrel through a hose, and a grain outlet of the grain sampling device is connected with a feeding port of the image acquisition device through a connecting pipe; the image acquisition device is connected with the control device, an image acquisition module of the control device controls the image acquisition device to acquire a grain image, and online calculation of the impurity rate and the breakage rate of the grain is realized on the grain image through a YOLOv8-Seg instance segmentation model of the image processing module in combination with an impurity rate and breakage rate mathematical model; finally, a detection result is displayed on a display screen in real time, visual numerical values can be provided for an operator of the combine harvester, and the operator is helped to adjust working parameters of the harvester. The method can be used for harvesting rice, wheat, oilseed rape and soybeans, and has wide applicability.

The invention discloses an application of a rape BnC05ERF378 gene in improving the waterlogging resistance of a plant. The nucleotide sequence of the rape BnC05ERF378 gene is as shown in SEQ ID NO. 1. According to the invention, a brand new stain-resistant gene BnC05ERF378 is cloned from rape for the first time, and is transferred into arabidopsis thaliana to construct a pure line plant and complete stain resistance identification. Experimental results show that the growth state of the transgenic BnC05ERF378 gene arabidopsis thaliana is remarkably superior to that of a wild type after waterlogging treatment, the transgenic BnC05ERF378 gene arabidopsis thaliana can normally bolt, the survival rate is increased from 12.5% to 37.5%, it is fully proved that the gene can enhance the survivability of the plant in a waterlogging environment by regulating the response mechanism of the plant to waterlogging stress, and the survival rate of the plant is increased from 12.5% to 37.5%. And a key guarantee is provided for stable growth of plants under high-humidity, waterflooding and other adverse conditions. According to the discovery and application of the waterlogging-resistant gene BnC05ERF378 disclosed by the invention, a clear functional gene target is provided for improvement of waterlogging resistance of crops. By constructing an expression vector containing the gene and combining mature technologies such as agrobacterium transformation, the gene can be stably introduced into a target plant, and a pure transgenic plant is obtained.

The invention provides a diglyceride oil composition for reducing lactone as well as a product and a preparation method thereof, and relates to the technical field of functional grease processing. The preparation method of the diglyceride oil composition comprises the following steps: (1) mixing corn oil and high oleic acid peanut oil, adding glycerol and water, then adding rhizomucor miehei lipase and pseudomonas onions lipase, and reacting to obtain a material A; (2) mixing sea buckthorn seed oil, acer truncatum seed oil and rapeseed oil, adding glycerol and water, adding candida antarctica lipase B and rhizopus oryzae lipase, and reacting to obtain a material B; and (3) mixing the material A and the material B, heating and centrifuging, taking an upper-layer oil sample, and distilling under a vacuum condition to obtain the diglyceride oil composition. The diglyceride oil composition prepared by the invention has a good effect in the aspect of reducing the internal fat.

The invention relates to the technical field of production of organic chili oil bean halves, and discloses an organic chili oil bean halve production process which comprises the following steps: cooking and curing organic broad beans, cooling to make koji, and carrying out heat preservation fermentation to obtain sweet halves; mixing and pickling the organic fresh chilies with sodium chloride to obtain low-salt chilli embryos; mixing the sweet petals with the low-salt chili embryos, and adding an emulsifying active medium to obtain soy sauce mash; performing staged temperature-controlled fermentation and normal-temperature solarization curing on the soy sauce mash; and grinding and sterilizing the fermented and cured material to obtain a finished product of the organic chili oil bean halves. The preparation method comprises the following steps: pre-dispersing rapeseed oil into fine liquid drops by using active yellow mustard micro powder; the calcium carbonate buffering agent releases calcium ions in response to the acidity change of the system, pectin in chili is induced to be subjected to a cross-linking reaction, a three-dimensional gel network is constructed in soy sauce mash, dispersed oil drops are physically embedded, the oil drop merging and migration resistance is increased, and the oil separation rate of the product in the storage process is reduced.

The invention discloses development and application of a molecular marker of a rape stain tolerance related gene BnaERF3, and belongs to the technical field of molecular biology. According to the invention, the BnaA06. ERF3 gene is screened on the basis of whole genome associated molecules, and is used as a key gene in QTL (qWL.A06) to participate in regulation and control of the waterlogging tolerance of the rape. A molecular marker related to the waterlogging tolerance of the brassica napus is developed aiming at the BnaA06. ERF3 gene, and tests show that the waterlogging tolerance of the brassica napus can be detected by utilizing the molecular marker, so that varieties containing waterlogging tolerance genes are subjected to auxiliary selection and breeding, and the waterlogging tolerance breeding process of the brassica napus is accelerated.

The utility model relates to a clamping and pulling type seedling taking device for oilseed rape pot seedlings, and relates to the technical field of oilseed rape planting. Comprising a supporting structure, a chain row conveying mechanism, a pressure plate recycling mechanism, a transmission system, a seedling pulling mechanism and a seedling clamping mechanism, the chain row conveying mechanism is arranged in the middle of the supporting mechanism, the pressure plate recycling mechanism is arranged above the chain row conveying mechanism, the seedling pulling mechanism is located on one side of the supporting mechanism, and the seedling clamping mechanism is arranged in the middle of the seedling pulling mechanism. The transmission system is in transmission connection with the chain row conveying mechanism, the seedling pulling mechanism and the seedling clamping mechanism and used for providing power for conveying, seedling pulling and seedling clamping of the seedling trays. According to the utility model, a seedling tray conveying function and a clamping and pulling seedling taking function are linked, so that the device takes seedlings orderly and accurately, and the efficient seedling taking operation effect is ensured.

The invention discloses a rape population canopy photosynthetic measurement method and device and a storage medium, and relates to the technical field of plant image processing. The method comprises the following steps: constructing a rape group point cloud data set with organ semantic information; training a rape group organ point cloud segmentation model by using the point cloud data set; segmenting the rape group organ point cloud by using the trained point cloud segmentation model; on the basis of the point cloud data of the rape group after organ segmentation in the whole growth period, rape group structure indexes are extracted; and constructing a photosynthetic capacity measurement index based on the rape population structure index, and obtaining photosynthetic capacity estimation of the whole-growth-period rape population. According to the method, high-throughput measurement of the photosynthetic parameters of the rape population can be accurately realized.

The invention relates to the technical field of plant polysaccharide extraction, in particular to a process for synergistically extracting rape pollen polysaccharide by a compound enzyme, which comprises the following steps: crushing rape pollen to 200-300 meshes by pulse airflow, performing microwave-ultrasonic degreasing by using a 85% ethanol solution, mixing with an acetic acid-sodium acetate buffer solution with the pH value of 4.8, and performing ultrasonic extraction to obtain a crude extract; the method comprises the following steps: adding a compound enzyme system which is immobilized by Fe3O4 (at) SiO2 nanoparticles by cellulase, pectinase, protease and beta-glucosidase according to a mass ratio of 2: 1: 1: 0.5, carrying out enzymolysis for 4 hours in a 50 DEG C constant-temperature water bath and a 15V / cm pulsed electric field, carrying out enzyme deactivation on enzymatic hydrolysate, carrying out gradient ethanol precipitation, carrying out ultrafiltration deproteinization, and carrying out vacuum pulsation freeze drying to obtain the polysaccharide. According to the method disclosed by the invention, the cell wall breakage rate and the enzymolysis efficiency are improved through pulsed airflow crushing, magnetic nano immobilized enzyme and pulsed electric field assistance, the polysaccharide yield reaches 7.32%, the purity is 91%, the beta-glucosidic bond proportion and the antioxidant activity are remarkably improved, the enzyme can be recycled and reused, the drying time is shortened, and efficient and low-consumption extraction is realized.

The invention discloses stenotrophomonas geniculata and application of fermentation liquor of the stenotrophomonas geniculata in prevention and treatment of rice false smut and sclerotinia rot of colza, and relates to the technical field of microorganisms, and the preservation number of the stenotrophomonas geniculata is CCTCC NO: M20232222. The stenotrophomonas geniculata provided by the invention is applied to prevention and treatment of false smut and sclerotinia rot, and is good in biocontrol effect and high in safety. The application of the invention can significantly reduce the application of biochemical agents in rice and rape diseases. In addition, the strain can also be applied to prevention and treatment of peach brown rot, and the effect is remarkable.

The invention relates to the technical field of agricultural biological information, in particular to a rape phenotype-genotype correlation analysis system based on deep learning, which comprises a phenotype image acquisition module, an image preprocessing module, an image phenotype feature extraction module, a multi-dimensional phenotype integration module, a deep learning model training module and a correlation analysis module, the image phenotypic feature extraction module creatively fuses a differential geometry theory and a deep learning technology, maps rape phenotypic features from an Euclidean space to a Riemannian manifold space through a multi-scale differential manifold representation network, and calculates feature correlation based on a geodesic distance by using a manifold attention weighting mechanism. The multi-scale geodesic integration module is adopted to realize organic fusion of phenotypic characteristics of different scales, the association analysis module adopts a multi-level linear regression model to analyze key agronomic characters of the rape, an association relationship between phenotypes and genotypes is established, and the phenotypic characteristic extraction accuracy under a complex background is improved by 35.2% compared with that of a traditional method.

The invention discloses application of a transcription factor BnaMYB52 in regulation and control of drought resistance of rape, and relates to the technical field of biology. The amino acid sequence of the transcription factor BnaMYB52 is as shown in any one of SEQ ID NO. 5 to 8. According to the invention, the negative regulation effect of the transcription factor BnaMYB52 in the drought resistance of the rape is disclosed for the first time, and a brand new molecular target and a genetic improvement strategy are provided for improving the drought resistance of the rape. Through in-depth study, it is found that knockout of the BnaMYB52 gene can significantly enhance the tolerance of rape plants to the drought environment. In addition, a new drought-resistant rape germplasm obtained after genetic modification of the gene shows excellent adaptive capacity in a drought stress environment. The technical breakthrough not only helps to solve practical problems faced by rape planting in arid regions, but also lays a solid theoretical foundation and technical support for cultivating high-yield and high-quality rape varieties adapting to extreme climate conditions.

The invention relates to the technical field of preparation methods of giant salamander peptide and eucommia ulmoides male flower tablets, in particular to a preparation method of giant salamander peptide and eucommia ulmoides male flower tablets. The giant salamander peptide and eucommia ulmoides male flower tablets comprise the following raw materials in parts by weight: 30-40 parts of giant salamander peptide powder, 20-25 parts of eucommia ulmoides male flowers, 10-15 parts of rape pollen, 10-15 parts of sea cucumber intestine peptide powder, 10-12 parts of a filler, 3-5 parts of an adhesive and 1-3 parts of a lubricant. According to the invention, the eucommia male flower contains geniposidic acid, chlorogenic acid, aucubin lignin, iridoid and phenylpropanoid natural active nutrient substances, flavonoid compounds, polysaccharide and other bioactive substances in the rape pollen, polysaccharide and peptide components in the sea cucumber intestine peptide powder, and the like. The andrias davidianus peptide powder is added into the andrias davidianus peptide powder, the andrias davidianus peptide powder cooperates with antioxidant peptide and immunomodulatory peptide in the andrias davidianus peptide powder to jointly form a rich bioactive substance library, and the components regulate the physiological functions of the human body from different angles, provide comprehensive nutrition support for the body and improve the fatigue resistance of the human body.

The invention provides a formula technology preparation method of a ruminant fattening soybean-meal-free daily ration feed which comprises the following components in percentage by mass: 30-40% of corn, 8-12% of wheat bran, 10-15% of corn distillers' grains, 8-12% of cottonseed meal, 5-10% of rapeseed meal, 3-5% of fish meal, 3-6% of corn protein powder, 5-8% of fermented bean dregs, 6-10% of microbial fermented straw, 2-4% of yeast culture and 1.5-2.5% of mineral element premix. 1-2% of a vitamin premix, 0.3-0.7% of a compound enzyme preparation and 0.5-1% of a microecological preparation. In the traditional feed granulation process, moisture, temperature and pressure are unstable, so that particles are uneven in hardness, large in dust amount and prone to mildewing. According to the method, the optimal granulation parameters are calculated by adopting MOPSO, and the particle uniformity and the storage stability are improved. The intelligent granulation optimization improves the water resistance of particles, reduces crushed materials, improves the palatability of the feed, reduces dust, prolongs the shelf life of the feed, and reduces the storage loss.

The invention discloses a portable rapeseed quality detection deep learning model lightweight method, which comprises the following steps: constructing a teacher model and a student model, and extracting local wave crest features and global time domain features from near infrared spectrum data of rapeseeds by using the teacher model and the student model, the teacher model and the student model output predicted values of the oil content of the rapeseeds; according to the difference between the predicted value output by the student model and the true value, the difference between the predicted values output by the teacher model and the student model, the difference between the local wave crest feature and the global time domain feature extracted by the teacher model and the student model, and the difference between the predicted values of each wave band of the teacher model and the student model; constructing combined distillation loss; and migrating knowledge of the teacher model to the student model through combined distillation loss, and predicting the oil content of the rapeseeds by using the student model after knowledge migration. And through multi-scale characteristic distillation and structured migration pruning, the operation efficiency of the model is remarkably improved on the premise of ensuring the prediction precision.

The invention belongs to the technical field of plant molecular markers, and particularly discloses an Indel molecular marker related to the salt tolerance of brassica napus and application of the Indel molecular marker. The marker is located at the position of an A01 chromosome scafoldA0133077947 of a brassica napus reference genome ZS11.v10, and the genotype of a polymorphic site is CTAA or C. The invention further discloses a method for identifying the marker of the brassica napus. Large-scale group phenotypic association analysis proves that the molecular marker is remarkably associated with the salt tolerance of the brassica napus in the germination period, and when the polymorphic site genotype is CTAA, the salt tolerance in the germination period is high; when the genotype of the polymorphic site is C, low salt tolerance in the germination period is shown. The invention finds that the polymorphic site is obviously associated with the salt tolerance of the brassica napus for the first time, provides a powerful molecular tool for solving the problems of difficulty in phenotype identification, long period and low efficiency in salt tolerance breeding of the brassica napus, can accurately screen salt-tolerant materials in the early stage, and greatly accelerates the breeding process of new varieties of the salt-tolerant brassica napus.

The invention belongs to the technical field of edible vegetable oil quality analysis, and discloses a construction method of a step-by-step temperature compensation model for thermal oxidation rapeseed oil quality detection based on an ultrasonic diagnosis technology. The invention aims to solve the problem that the environment temperature change can influence the sound wave pulse signal when the rapeseed oil quality is detected by utilizing an ultrasonic diagnosis technology. According to the method, a plurality of partial least squares (PLSR) or random forest method (RF) models are respectively established by adopting each integer temperature in a temperature range of 22-32 DEG C and correspondingly acquired ultrasonic data parameters in combination with physical and chemical data parameters acquired at the temperature; the prediction pertinence and accuracy of the temperature compensation model are improved in a mode of constructing a plurality of sub-models, and meanwhile, a training set, an internal verification set and an external verification set are introduced to perform multiple verification evaluation on the model, so that the prediction performance and stability of the model are ensured, and a quality index prediction value output by the model is closer to an actual quality index; and the accuracy and effectiveness of detection data are ensured.

The invention provides an application of a Brassica napus gene BnSKIP31 in prevention and control of sclerotiniose, which is an application in obtaining a disease-resistant enhanced rape material by creating a gene to edit rape. According to the invention, CRISPR gene knockout rape of BnSKIP31 is constructed, the negative regulation function of the gene on sclerotiniose resistance is disclosed for the first time, and application of the BnSKIP31 gene to acquisition of sclerotiniose rape materials with increased sclerotiniose resistance by creating CRISPR gene knockout rape is provided. The BnSKIP31 gene provided by the invention is a new gene resource suitable for creating and breeding new sclerotiniose-resistant rape materials and new varieties, and the created rape germplasm has lasting disease resistance.

The invention belongs to the technical field of microbial fermentation, and particularly relates to a method for preparing rapeseed meal polypeptide through mixed bacteria combined fermentation of rapeseed meal. The mixed strain composition suitable for fermenting the rapeseed dregs is obtained through screening, and the rapeseed dregs are subjected to combined fermentation by adopting the mixed strain composition, so that the content of soluble peptides in a fermentation product can be increased, and the content of anti-nutritional factors such as glucosinolates in the fermentation product can be reduced; the antioxidant performance of the rapeseed meal fermentation peptide can be improved, and the rapeseed meal fermentation peptide feed has better nutritional efficacy and feed quality and is wide in application prospect.

The invention provides diglyceride oil with a blood sugar reducing effect and a preparation method of the diglyceride oil, and relates to the technical field of functional grease processing. The preparation method comprises the following steps: mixing sea buckthorn seed oil, acer truncatum seed oil, rapeseed oil, corn oil and high oleic acid peanut oil, and adding glycerol and water to obtain a material A; adding onion pseudomonas lipase and candida antarctica lipase B into the material A, and carrying out primary reaction to obtain a material B; adding candida lipolytica lipase and candida antarctica lipase B into the material B, and carrying out a second reaction to obtain a material C; adding thermomyces lanuginosus lipase into the material C, and carrying out a third reaction to obtain a material D; and treating the reacted material D to obtain the diglyceride oil. The diglyceride oil prepared by the invention has a good effect in the aspect of reducing blood sugar.

The invention relates to a heterocyclic pyrimidinedione compound and application thereof in preparation of pesticides and medicines, in particular to the heterocyclic pyrimidinedione compound and application thereof in preparation of herbicides and plant virus resisting agents, and application in preparation of medicines for resisting fungal infection, especially candida auricula infection. The compound provided by the invention has good inhibition effect on KARI enzymes from different sources. Under the dosage of 100g / mu, the weeding effect of the compound provided by the invention on rape during stem and leaf treatment can reach 100%. The compound disclosed by the invention has a good inhibition effect on a candida auricula 12766 strain, and the minimum inhibition concentration (MIC) value of the compound can reach 0.31 mg / L when a YNB culture medium is used. The in-vivo passivation effect of the compound on the tobacco mosaic virus TMV under the concentration of 500 mg / L can reach 64.2%.

The invention relates to the technical field of gene engineering, in particular to application of BnBRC1 and BnGA5 genes in improvement of branches and plant heights in plants. The invention provides application of BnBRC1 and BnGA5 knockout genes in increasing the number of plant branches and reducing the plant height. The construction of BnBRC1 and BnGA5 (hereinafter referred to as BG double knockout) strains shows that compared with a wild type, the BG double knockout strains endow the rape with multi-branching and dwarfing phenotypes, and shows that the BnBRC1 and BnGA5 genes have a negative regulation effect on increasing the number of branches and have a positive regulation effect on increasing the plant height, and the BnBRC1 and BnGA5 genes have a positive regulation effect on increasing the plant height. The molecular biology principle support is provided for improving the branching capacity and reducing the plant height by constructing a transgenic plant, and the method can be used for cultivating a new rape variety.

The utility model relates to the technical field of oilseed rape cutting and drying, in particular to an oilseed rape cutting and drying machine which comprises a machine body, a transverse mounting plate, a vertical mounting plate, an adjusting mechanism, a cutting mechanism and a feeding mechanism. The cutting mechanism comprises a vertical fixed cutter, a vertical movable cutter, a transverse fixed cutter, a transverse movable cutter, a telescopic assembly and a transmission assembly, the feeding mechanism comprises a driving assembly, two strip-shaped grooves, three sets of stirring assemblies, a plurality of grain lifters and a plurality of grain pulling wheels, the transverse fixed cutter and the transverse movable cutter are arranged, in the walking process of the cutter-rower, cutting of oilseed rapes can be achieved, and the cutting efficiency is improved. Meanwhile, through cooperation of the feeding mechanism, the cut oilseed rape can be conveyed, so that the cut oilseed rape can be laid and aired on a field on one side of the cutter-rower, cutting and airing of the oilseed rape are achieved, meanwhile, orderly placement of the cut oilseed rape can be achieved, and the whole oilseed rape cutting, conveying and laying process is completed.

The invention discloses an oilseed rape pot seedling transplanter, and relates to the technical field of oilseed rape planting. Comprising a power headstock, a seedling planting mechanism, a support, a transmission shaft and first side plates, the support is connected with the power headstock, the transmission shaft is in transmission connection with the power headstock, the transmission shaft is rotationally connected with the support, the two ends of the transmission shaft are each provided with one first side plate, and a plurality of rotating shafts are arranged between the two first side plates in parallel; each rotating shaft is provided with a plurality of duckbilled planters, and each duckbilled planter is provided with a switch assembly used for controlling the duckbilled planter to be opened and closed, a seedling feeding mechanism and a seedling taking mechanism. According to the rape pot seedling transplanter provided by the invention, automatic transplanting of rape pot seedlings can be realized, the transplanting efficiency of the rape pot seedlings is improved, and labor is saved.

The invention belongs to the field of agricultural science and technology, and relates to a growth-promoting stain-resistant rape seed pelleting agent and a preparation method and application thereof, the pelleting agent comprises the following components by mass: 30-50% of an inert filler, 15-25% of a growth-promoting substance, 15-30% of a stain-resistant functional component, 8-15% of a bactericide and insecticide composition, and the balance of an adhesive; the pelleting agent can promote germination of rape seeds and growth of seedlings, and the stain-resistant functional component can significantly improve the stain resistance of the rape seeds and the seedlings; the bactericide and insecticide combination effectively prevents and treats diseases and insect pests in the seedling stage, reduces the pesticide consumption, improves the agronomic characters and quality of rape, does not affect the germination rate, improves the growth vigor in the seedling stage, and facilitates mechanical seeding; after the pelleting agent is used, the yield of the rape per mu can be increased by 8-15 kg, the net income is increased by 49.26-91.26 yuan, and the pelleting agent is simple in preparation process, low in cost and easy to popularize and use and has good economic benefits and social benefits.

The invention discloses application of oilseed rape chitinase EP3 and a coding gene thereof in improving clubroot resistance of cruciferous plants, and belongs to the technical field of molecular biology. The invention discloses the application of the rape chitinase EP3 in improving the clubroot resistance of plants for the first time. The amino acid sequence of the rape chitinase EP3 is as shown in SEQ ID NO.2; the nucleotide sequence of the coding gene EP3 is shown as SEQ ID NO. 1. Experimental results show that by preparing agrobacterium containing an EP3 gene overexpression vector and using a treating fluid containing the agrobacterium to perform seed soaking and root irrigation treatment, the EP3 gene is overexpressed in a rape plant, and the clubroot resistance of the rape can be remarkably improved. The invention provides a new gene resource and a safe, efficient, simple, convenient and universal prevention and treatment method for preventing and treating clubroot of cruciferous plants, and has remarkable agricultural application value and wide application prospect.

The invention relates to the technical field of molecular biology, in particular to an SNP (Single Nucleotide Polymorphism) molecular marker related to the brassica napus branching number, a primer and application. The SNP molecular marker related to the position of the rape genome chrA09: 46507122 comprises a nucleotide sequence as shown in SEQ ID NO. 1 and a nucleotide sequence as shown in SEQ ID NO. 2; the basic group at the chrA09: 46507122 position of the rape genome is polymorphic, and the basic group is T or C. The invention further discloses a method for identifying the rape genome chrA09: 46507122 position of the rape genome. The primer is a primer for amplifying the SNP molecular marker. The application is the application of the SNP molecular marker in rape multi-branch character breeding. The method can provide possibility for genetic improvement of the branch number character of the oilseed rape, and further facilitates cultivation of high-yield oilseed rape varieties.

The utility model discloses a rapeseed stir-frying machine, which relates to the technical field of oil product processing and comprises a base, seed stir-frying components are fixedly mounted at the left and right parts of the upper end of the base, and a feed filtering component is fixedly mounted at the right part of the top of each seed stir-frying component. According to the rapeseed frying machine disclosed by the utility model, rapeseeds in the heating furnace can be turned and fried through the seed frying assembly, and can be conveyed to the left part of the inner cavity of the heating furnace during frying. Rapeseeds entering the heating furnace can be filtered and screened in advance through the feeding filtering assembly, and impurities in the rapeseeds are separated and collected. Rapeseeds discharged by the heating furnace can be guided onto the electric conveying belt through the guide plate by the conveying air-cooling assembly, so that the rapeseeds can be subjected to air cooling in the tiled conveying process, and the temperature of the fried rapeseeds is rapidly reduced by a plurality of air spraying heads, so that the oxidation reaction can be inhibited, and the damage of high temperature to nutritional ingredients can be reduced.