The invention relates to the technical field of
plant tissue culture, in particular to a
stellaria yunnanensis tissue rapid propagation method and application thereof.The
stellaria yunnanensis tissue rapid propagation method comprises the steps that newly-germinated twigs are
cut from a
stellaria yunnanensis
plant, cleaned and sterilized, and sterilized explants are inoculated into an induction culture medium according to the
plant growth polarity to be cultured for 15-25 d;
cutting the induced cluster buds into single buds, inoculating the single buds into a proliferation culture medium, culturing for 25-35 days,
cutting off leaves after the cluster buds are
cut into single buds, inoculating the single buds into a rooting culture medium, culturing for 20-30 days,
transplanting seedlings subjected to rooting culture into a
mixed matrix in a hole tray, culturing for 40-60 days, and
transplanting. According to the method, the disinfection
pollution rate of the explants can be controlled within 18%, and the disinfection death rate is controlled within 15%; the explant induction rate reaches 85% or above, the multiplication coefficient reaches 11.6 or above, the rooting rate reaches 98% or above, the
survival rate after
transplanting reaches 90% or above, and transplanting can be conducted after the culture period is only 100-150 days.