17 results about "Primary dysmenorrhoea" patented technology

The application discloses a cassia twig composition and a preparation method and application thereof in preparing medicines for treating gynecological diseases, and relates to the technical field of medicines.The preparation method comprises the following steps: (1) after mixing cassia twig and licorice, alcohol ultrasonic treatment is conducted to obtain alcohol extraction liquid and dregs; (2) after mixing the dregs, white peony root, Chinese date and fresh ginger, water extraction is conducted to obtain water extraction liquid; (3) the alcohol extraction liquid is concentrated and treated by macroporous resin to obtain eluent; and (4) the eluent and the water extraction liquid are combined, concentrated and dried to obtain the cassia twig composition.The macroporous resin treatment is as follows: the volume of the sample liquid is 5BV-10BV, the sample liquid is loaded at a flow rate of 2BV / h-8BV / h, 3BV-7BV of 70%-80% ethanol is used for elution at a flow rate of 1BV / h-5BV / h.The prepared cassia twig composition can better treat cold coagulation and blood stasis dysmenorrhea, and more choices are provided for the treatment of primary dysmenorrhea and other gynecological diseases.

The application belongs to the technical field of microorganisms, and provides a Streptococcus salivarius subsp. thermophilus HX-ST36, which has a preservation number of CGMCC NO. 32955. The Streptococcus salivarius subsp. thermophilus provided by the application can ferment Astragalus membranaceus Bunge-Angelica sinensis. In a cold coagulation and blood stasis type primary dysmenorrhea model mouse, the fermentation product thereof can significantly inhibit the writhing response, and can realize the effects of relieving dysmenorrhea and tonifying blood by reducing the levels of prostaglandin PGF2 and vasopressin ADH in uterine tissue and increasing the hemoglobin (HGB) content. The effect of the fermentation product in removing dampness is verified by improving the dampness signs of a crowd. The application further provides a probiotic preparation and a preparation method of a probiotic fermentation product. The preparation method of the probiotic preparation and the probiotic fermentation product is simple and can be produced on a large scale.

The invention relates to the technical field of traditional Chinese medicine compositions, in particular to a traditional Chinese medicine composition for improving lung deficiency type primary dysmenorrhea and a preparation method and application thereof. The traditional Chinese medicine composition specifically comprises multiple traditional Chinese medicine components including rhizoma polygonati, Chinese yam, rhizoma smilacis glabrae, radix salviae miltiorrhizae, yam rhizome, codonopsis pilosula, radix ophiopogonis from Sichuan of China, exocarpium citri grandis, spina date seeds, radix bupleuri, radix clematidis, morinda officinalis, rhodiola rosea, peach kernels, caulis spatholobi, southern apricot kernels and prepared rehmannia roots. The composition effectively relieves pain and improves accompanying symptoms through multi-target synergistic effects of promoting blood circulation to remove blood stasis, reinforcing qi and nourishing lung, warming kidney and strengthening spleen, regulating qi and relieving pain and the like. The formula follows the monarch, minister, assistant and guide theory of traditional Chinese medicine, is rigorous in compatibility, can comprehensively regulate qi, blood and viscera functions of a patient, is especially suitable for dysmenorrhea caused by deficiency of lung qi and unsmooth qi and blood circulation, and shows the advantages of overall treatment of traditional Chinese medicine. The preparation method is stable in process and easy to implement, and a safe and effective new choice for traditional Chinese medicine treatment is provided for lung deficiency type primary dysmenorrhea.

The invention discloses a method for evaluating the analgesic effect of angelica sinensis stomachache dropping pills, belongs to the technical field of efficacy evaluation, and aims to solve the problem that no scientific method is provided for efficacy verification of dysmenorrhea traditional Chinese medicine preparations in the formula of angelica sinensis. The method comprises the steps of experimental mouse selection, acetic acid body twisting model test, hot plate pain model test and xylene inflammation model test. Selecting an experimental rat, and testing the primary dysmenorrhea model constructed by estradiol and oxytocin. The method comprises the following steps: constructing an analgesic effect evaluation model, and verifying analgesic and anti-inflammatory effects of angelica sinensis stomachache relieving; pharmacodynamic mechanism analysis is further carried out on the angelica sinensis stomachache dropping pill for simulating primary dysmenorrhea, it is revealed that the content of serum E2, IL-6, PROG, beta-EP and TNF-alpha of primary dysmenorrhea model rats treated by the angelica sinensis stomachache dropping pill has an adjusting effect, the effect of relieving dysmenorrhea can be achieved, the inflammation effect can be relieved by inhibiting NF-kappa B and MAPK channels, and the pain behavior can be relieved.

The invention relates to the technical field of microorganisms, and particularly discloses lactobacillus acidophilus PIS4 capable of improving dysmenorrhea and application of the lactobacillus acidophilus PIS4. The lactobacillus acidophilus PIS4 is separated from genital tracts of healthy women, and is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.34554, and the preservation date is May 15, 2025. The lactobacillus acidophilus PIS4 is used for preparing the lactobacillus acidophilus PIS4. The lactobacillus acidophilus strain PIS4 provided by the invention is proved to be capable of inhibiting the gene expression of prostaglandin E synthase 2 (pges-2) and reducing the synthesis of prostaglandin E2 and prostaglandin F2alpha, and has effective relieving and improving effects on primary dysmenorrhea.

The invention relates to a preparation method of a stable rat model for primary dysmenorrhea with syndrome of cold coagulation and blood stasis, belongs to the technical field of animal model construction, and particularly relates to a construction method of a rat model for primary dysmenorrhea according with the pathogenesis characteristics of cold coagulation and blood stasis in traditional Chinese medicine. The model simulates the pathological process of cold evil invasion and uterus stasis and combines a modern medical hormone intervention means to realize the construction of a'disease and syndrome combined 'animal model, and is mainly used for the anti-dysmenorrhea mechanism research and drug screening of traditional Chinese medicine compound, acupuncture and other therapies. The adopted preparation method comprises the steps that 0.4 mg of estradiol benzoate is subcutaneously injected to each rat every day for 12 days, the injection dosage of the first day and the injection dosage of the last day are increased to 2 times, the rats are placed in the environment of-20 DEG C after injection is completed, epinephrine is injected twice every day from the 6th day of molding, and 2 U of oxytocin is injected to each rat intraperitoneal cavity 24 hours after the epinephrine is injected for the last time. The animal model obtained through the method is stable in phenotype and good in repeatability, compared with pure cold or pure irritation, more remarkable pain behaviors and biochemical changes can be obtained in a short period through combination with epinephrine, and pharmacodynamic screening is facilitated.

The invention belongs to the technical field of traditional Chinese medicine application, and particularly relates to a state-target combined dysmenorrhea square patch. The traditional Chinese medicine is prepared from the following medicines in parts by weight: 8-12 parts of cinnamon, 10-14 parts of baked ginger, 8-12 parts of angelica sinensis, 8-12 parts of ligusticum wallichii, 8-12 parts of radix paeoniae rubra, 8-12 parts of peach kernels, 8-12 parts of rhizoma cyperi, 10-14 parts of trogopterus dung, 10-14 parts of pollen typhae, 10-14 parts of rhizoma corydalis, 2-4 parts of amber, 8-12 parts of fennel, 18-22 parts of lindera aggregata and 13-17 parts of motherwort. The state-target combined dysmenorrhea plaster provided by the invention can improve the uterus microcirculation of a patient with primary dysmenorrhea and regulate the inflammation state, can significantly improve the severity of the patient with primary dysmenorrhea, and can reduce the severity and duration of dysmenorrhea symptoms.

This invention relates to the field of traditional Chinese medicine (TCM) technology, and more particularly to a TCM composition and pharmaceutical preparation for treating dysmenorrhea. The TCM composition includes Astragalus membranaceus, Codonopsis pilosula, stir-fried Atractylodes macrocephala, Angelica sinensis, Paeonia lactiflora, Cinnamomum cassia, Zingiber officinale (dried), Cyperus rotundus (processed with vinegar), Corydalis yanhusuo, and Glycyrrhiza uralensis (raw). The TCM composition provided by this invention has a simple formulation, addresses both the symptoms and the root cause, has a definite curative effect, and is highly safe. Using the TCM composition and pharmaceutical preparation provided by this invention to treat primary dysmenorrhea caused by cold stagnation and blood stasis, and qi and blood deficiency, it has a synergistic effect of "warming the meridians and dispelling cold, tonifying qi and nourishing blood, and promoting blood circulation and relieving pain."

This invention belongs to the field of biomedicine, specifically relating to the use of gemmaconazole in the preparation of drugs for treating primary dysmenorrhea. Gemarazole of this invention can significantly reduce the release of prostaglandins in uterine tissue, thereby relieving uterine smooth muscle spasms, reducing endometrial congestion and inflammatory responses, and thus alleviating pain, thus playing a role in treating primary dysmenorrhea. This provides a new option for clinical treatment of primary dysmenorrhea and has good application prospects.

The application discloses a uterus rhythmic contraction organ chip simulating primary dysmenorrhea and a preparation method thereof, and belongs to the technical field of organ chips. The uterus rhythmic contraction organ chip comprises, from top to bottom, an upper chamber layer, a middle porous membrane and a lower chamber layer. The upper chamber layer is internally provided with five parallel microchannels, including a first channel, a second channel and a third channel arranged in sequence in the middle, and a fourth channel and a fifth channel arranged on both sides. The adjacent microchannels are separated by microcolumns arranged at intervals, and the gap between the adjacent microcolumns constitutes a microenvironment interaction path between the microchannels. The bottom of the third channel penetrates through the upper chamber layer to form an open bottom end. The lower chamber layer is internally provided with an airway chamber located directly below the third channel. The middle porous membrane blocks the open bottom end of the third channel and physically isolates the airway chamber from the third channel.

The application discloses a ginger and jujube group formula, a preparation method thereof and application of the ginger and jujube group formula in prevention and treatment of cold syndrome primary dysmenorrhea products. The ginger and jujube group formula is composed of ginger, jujube, white peony root and fried licorice in a specific ratio, and an extract can be prepared by using a water extraction, alcohol extraction or water extraction and alcohol precipitation process. Experiments prove that the group formula and the extract can improve the cold and blood stasis signs of a cold syndrome primary dysmenorrhea model mouse, reduce writhing responses, reduce uterine and ovarian pathological injuries, down-regulate serum and uterine tissue inflammatory factor levels, and regulate endocrine and microcirculation disorders. The application has the advantages of scientific compatibility, definite curative effect, stable preparation process, high safety, suitability for long-term conditioning and the like, and is used for preventing and treating cold evil invasion and cold and blood stasis type primary dysmenorrhea.

An analgesic moxibustion stick for treating primary dysmenorrhea caused by cold stagnation and blood stasis effectively solves the problem of preparing medication for this type of dysmenorrhea. The analgesic moxibustion stick consists of a medicinal core and a paper roll. The medicinal core is made by mixing a compound powder of traditional Chinese medicine with moxa wool and stirring thoroughly. The compound powder of traditional Chinese medicine is composed of warming and dispersing cold herbs, blood-activating and stasis-removing herbs, meridian-guiding herbs, and transdermal adjuvants. The warming and dispersing cold herbs are made by mixing cinnamon, dried ginger, and evodia rutaecarpa powder; the blood-activating and stasis-removing herbs are made by mixing angelica sinensis, chuanxiong rhizome, red peony root, and peach kernel powder; the meridian-guiding herbs are made by mixing asarum and cyperus rhizome powder; and the transdermal adjuvant is borneol. The paper roll is made of double-layered mulberry bark paper rolled using conventional methods. The medicinal core is then placed inside the paper roll. This invention uses abundant raw materials, has a simple preparation method, is easy to produce, and has a scientifically sound drug formulation. It has the effects of warming yang and dispersing cold, activating blood and unblocking collaterals, and has good efficacy, making it valuable for practical application.

The invention provides a pharmaceutical composition for treating teenager dysmenorrhea and a preparation method and application thereof, the pharmaceutical composition is prepared by compatibility of 23 traditional Chinese medicines such as cassia twig, white peony root, pollen typhae, trogopterus dung, dragon's blood and the like, and a composite regulatory peptide and a micro-ecological regulator are innovatively added. Experiments show that the composition significantly inhibits a COX-2 / PGE2 pathway and reduces the level of inflammatory factors such as IL-6 and TNF-alpha through a multi-target synergistic effect, and in an animal model, the twisting frequency is reduced by 66%, the incubation period is prolonged by 142%, the COX-2 expression is reduced by 63%, and the abundance of intestinal bifidobacterium is improved by 20%. Clinical data show that the cure rate in three menstrual cycles reaches 85%, the recurrence rate is only 5%, and the traditional Chinese medicine composition is superior to the traditional western medicine. The preparation method adopts a process of combining gradient temperature decoction with 4-6 DEG C low-temperature active ingredient addition. The ointment formulation solves the problems of poor taste and low compliance of decoction, and has no adverse effect. Traditional dysmenorrhea treatment limitation is broken through, and a safe and efficient treatment scheme is provided for primary dysmenorrhea of teenagers through a synergistic interaction mechanism of the medicines.

This invention provides a composition with menstrual regulating and blood-activating effects and its application in the preparation of external products, relating to the field of biomedical technology. This invention utilizes a composition composed of Chinese herbal raw materials including *Cephalotaxus fortunei* bark, star anise, *Erigeron breviscapus*, *Piper longum*, *Caulis thunbergii*, *Radix Achyranthis Bidentatae*, *Radix Scutellariae*, *Radix Achyranthis Bidentatae*, *Radix Panax notoginseng*, *Radix Bambusae in Taeniam*, and *Radix Achyranthis Bidentatae* to prepare a traditional Chinese medicine acupoint patch for treating or improving primary dysmenorrhea. The herbal acupoint patch can inhibit the expression of inflammatory factors, downregulate the expression level of COX-2, thereby inhibiting PGF2α, increasing the level of PGE2, and reducing uterine tissue inflammation, achieving multi-target treatment of primary dysmenorrhea. The herbal acupoint patch utilizes the power of the medicine through the acupoint, and the acupoint utilizes the properties of the medicine. The meridians are the channels, and the acupoints are the gateways. The medicinal power directly reaches the Chong and Ren meridians and the uterus, so that cold is warmed and dispelled, blood stasis is cleared and eliminated, and qi and blood flow smoothly, thus curing dysmenorrhea. It plays a role in warming the meridians and dispelling cold, promoting qi and blood circulation, removing blood stasis and relieving pain, providing a new direction for the external treatment strategy of traditional Chinese medicine for primary dysmenorrhea.

The application discloses a deer source extract and application thereof in preparation of a medicine for treating primary dysmenorrhea, and belongs to the technical field of medicines. The deer horn extract is prepared from deer horn through alkali extraction and acid precipitation, and is purified through molecular weight cut-off, and four P1-P4 deer horn polypeptides with specific amino acid sequences are obtained through further multi-dimensional activity screening. The deer horn polypeptide can play a synergistic effect on multiple targets through regulating uterine tissue prostaglandin balance, inhibiting abnormal contraction of uterine smooth muscle, reducing local inflammatory response, improving ischemia and hypoxia injury, and regulating pain signal transmission, and effectively improves symptoms such as spasm and pain of primary dysmenorrhea. The application also prepares various dosage forms such as tablets and capsules by taking the deer horn extract or the deer horn polypeptide as a main active ingredient, and can meet the needs of different scenes such as acute symptom relief and long-term periodic conditioning, has high safety, good patient compliance, and provides a safe and effective natural intervention scheme for primary dysmenorrhea.

The invention provides application of ergothioneine in preparation of a composition for relieving primary dysmenorrhea, and belongs to the technical field of compositions. The composition comprises the following components in parts by weight: 20-500 parts of ergothioneine, 50-350 parts of magnesium threonate and 10-50 parts of a Bile berry extract, the Bile berry extract is adopted as a plant extract to be mixed with ergothioneine and magnesium salt for use, so that the composition has the effects of nourishing blood, warming channels, dispersing blood stasis and relieving pain, and has a good curative effect on treating and relieving dysmenorrhea.

The invention provides a traditional Chinese medicine composition for relieving primary dysmenorrhea and a preparation method of the traditional Chinese medicine composition. The traditional Chinese medicine composition comprises codonopsis pilosula, angelica sinensis, ligusticum wallichii, salvia miltiorrhiza, raw cattail, curcuma zedoary, lindera aggregata, myrrh and corydalis tuber. The traditional Chinese medicine composition provided by the invention overcomes the defects of complex formula, unstable drug effect, inconvenience in use and the like in the prior art, and provides a more effective, safer and more convenient treatment choice for dysmenorrhea patients.