32 results about "Primary dysmenorrhoea" patented technology

The invention belongs to the technical field of microorganisms, and provides streptococcus salivarius subsp. Thermophilus HX-ST36, and the preservation number of the streptococcus salivarius subsp. Thermophilus HX-ST36 is CGMCC NO.32955. The streptococcus salivarius subsp. Thermophilus provided by the invention can be used for fermenting astragalus membranaceus-angelica sinensis. In a cold coagulation and blood stasis type primary dysmenorrheal model mouse, a fermentation product can remarkably inhibit the writhing reaction, and the effects of relieving dysmenorrheal and enriching blood can be achieved by reducing the levels of prostaglandin PGF2 and vasopressin ADH in uterine tissue and increasing the content of hemoglobin (HGB). The dampness clearing effect of the fermentation product is verified by improving the heavy physical sign of moisture through crowd experiments. The invention also provides a preparation method of the probiotic preparation and the probiotic fermentation product. The preparation methods of the probiotic preparation and the probiotic fermentation product are simple, and large-scale production can be realized.

The invention belongs to the technical field of food microorganisms, and particularly relates to lactobacillus rhamnosus ZJUIDS07 with the effect of relieving primary dysmenorrhea and application of the lactobacillus rhamnosus ZJUIDS07. The invention discloses an application of lactobacillus rhamnosus ZJUIDS07 in preparation of a medicine with an effect of relieving primary dysmenorrhea, and the preservation number of the lactobacillus rhamnosus ZJUIDS07 is CGMCC (China General Microbiological Culture Collection Center) NO.23996. The lactobacillus rhamnosus ZJUIDS07 can be used for preparing a medicine with an effect of relieving primary dysmenorrhea. According to the lactobacillus rhamnosus ZJUIDS07, the level of PGE2PGF2a is regulated and controlled, so that the effect of relieving dysmenorrhea is achieved, and the lactobacillus rhamnosus ZJUIDS07 can be used for regulating and controlling the level of PGE2PGF2a.

The invention discloses a cassia twig composition, a preparation method thereof and application of the cassia twig composition in preparation of medicine for treating gynecological diseases, and relates to the technical field of medicines.The preparation method comprises the following steps that 1, cassia twigs and licorice roots are mixed, alcohol is added, ultrasonic treatment is conducted, and an alcohol extract and medicine residues are obtained; (2) mixing the medicine residue, radix paeoniae alba, jujube and ginger, and performing water extraction to obtain a water extract; (3) concentrating the alcohol extract, and treating the concentrated alcohol extract with macroporous resin to obtain an eluent; and (4) combining the eluent and the water extract, concentrating, and drying to obtain the product. The macroporous resin treatment comprises the following steps: loading a sample at the flow rate of 2-8 BV / h by the volume of a loading liquid of 5-10 BV, and eluting with 70-80% ethanol of 3-7 BV at the flow rate of 1-5 BV / h. Compared with the prior art, the cassia twig composition prepared by the invention can better treat dysmenorrhea caused by congealing cold and blood stasis, and provides more choices for treatment of gynecological diseases such as primary dysmenorrhea and the like.

The disclosed composition consists of 15 mg Ferula asafetida (Hing) oil as an active ingredient, 85 mg Frankincense oil and 400 mg coconut oil as in-active ingredients in an enteric coated HPMC capsule form. The process of preparation consists of steps of blending asafetida (hing) oil with Frankincense oil and coconut oil followed by dispensing the blended oils into empty HPMC capsule shell, which are automatically filled in liquid filling machine. Further a banding solution containing 0.025 ml HPMC (surfactant), 2.8 mg IPA (Binding Agent) and 0.03 mg Chocolate brown color (colorant) are filled into the bath of band sealing machine at room temperature where the hing oil containing capsules are band sealed. The disclosed oral formulation in the form of HPMC capsule is natural, low cost, highly efficacious and quick in management primary dysmenorrhea.

The application discloses a cassia twig composition and a preparation method and application thereof in preparing medicines for treating gynecological diseases, and relates to the technical field of medicines.The preparation method comprises the following steps: (1) after mixing cassia twig and licorice, alcohol ultrasonic treatment is conducted to obtain alcohol extraction liquid and dregs; (2) after mixing the dregs, white peony root, Chinese date and fresh ginger, water extraction is conducted to obtain water extraction liquid; (3) the alcohol extraction liquid is concentrated and treated by macroporous resin to obtain eluent; and (4) the eluent and the water extraction liquid are combined, concentrated and dried to obtain the cassia twig composition.The macroporous resin treatment is as follows: the volume of the sample liquid is 5BV-10BV, the sample liquid is loaded at a flow rate of 2BV / h-8BV / h, 3BV-7BV of 70%-80% ethanol is used for elution at a flow rate of 1BV / h-5BV / h.The prepared cassia twig composition can better treat cold coagulation and blood stasis dysmenorrhea, and more choices are provided for the treatment of primary dysmenorrhea and other gynecological diseases.

The invention discloses a traditional Chinese medicine composition for treating primary dysmenorrhea and a preparation method, and belongs to the field of medical treatment and public health. The composition is based on the wind-expelling and pain-relieving theory and clinical experience of the nationwide nationwide traditional Chinese medicine weekly inscription instructor, is prepared from 10 to 20g of notopterygium root, 10 to 20g of divaricate saposhnikovia root, 10 to 20g of radix angelicae, 10 to 30g of radix angelicae pubescentis, 10 to 20g of rhizoma corydalis, 10 to 30g of radix paeoniae alba, 10 to 20g of ligusticum wallichii, 10 to 20g of rhizoma cyperi and 10 to 30g of radix clematidis, and has the effects of expelling wind, relieving pain, warming and activating meridians and dredging collaterals. The preparation method comprises the steps of raw material pretreatment, extraction, concentration and preparation forming, and can be prepared into decoction, granules and other dosage forms. The scheme aims at the pathogenesis of primary dysmenorrhea of'wind pathogen latent in uterus and blocking menstrual blood ', solves the problems of poor long-term curative effect and repeated attack of pain in existing treatment, and is remarkable in clinical curative effect, high in safety and suitable for treatment of primary dysmenorrhea.

The invention discloses a compound essential oil for relieving primary dysmenorrhea and a preparation method thereof. The compound essential oil for relieving primary dysmenorrhea includes limonene, gingerol, α-terpinene acetate, β-sesquiphellandrene, eucalyptol, p-cymene, β-phellandrene, γ-terpinene, α-pinene, β-bisabolene, α-curcumene, trans-caryophyllene, camphene, β-pinene, myrcene, linalool, cis-11-eicosenoic acid, 3-thujacene, α-farnesene, sabinene, 3-carene, nonanoic acid, α-copaene, β-elemene. The present invention has a significant effect of relieving primary dysmenorrhea, and has good safety. In addition, essential oil preparations are easier to penetrate into the skin than cream preparations, and are more likely to activate cells after being absorbed by the skin.

The invention provides a traditional Chinese medicine composition for treating primary dysmenorrhea and a preparation method and application thereof.The traditional Chinese medicine composition is prepared from, by weight, 3-15 parts of rhizoma corydalis, 6-15 parts of angelica sinensis, 3-15 parts of ligusticum wallichii, 3-10 parts of rhizoma zingiberis, 1.5-6 parts of fennel and 3-12 parts of cassia twigs, and the traditional Chinese medicine composition is prepared from, by weight, 3-15 parts of rhizoma corydalis, 6-15 parts of angelica sinensis, 3-15 parts of ligusticum wallichii, 3-10 parts of rhizoma zingiberis, 1.5-6 parts of fennel and 3-12 parts of cassia twigs. The traditional Chinese medicine composition is prepared by grinding and uniformly mixing the raw materials, all the components have a synergistic effect, and the traditional Chinese medicine composition has the effects of resisting inflammation, resisting oxidative stress, regulating the estrogen level, improving the pathological morphology of the uterus and easing pain, and has a remarkable effect on treating primary dysmenorrhea; the traditional Chinese medicine composition is used for treating primary dysmenorrhea in the form of a traditional Chinese medicine patch, and by combining traditional Chinese medicine application and thermal therapy, local skin blood flow can be remarkably increased, the diffusivity and solubility of medicine in cuticle can be improved, the skin absorption capacity can be enhanced, the medicine absorption and permeation amount can be increased, local blood circulation can be improved, and pain can be relieved; and no anaphylactic reaction is caused to the skin.

The invention discloses an observation method for discussing the curative effect of a graphene acupoint patch on primary dysmenorrhea based on the acupoint theory, and mainly relates to the technical field of traditional Chinese medicine external treatment. Comprising the following steps of: screening and grouping subjects: formulating a primary dysmenorrhea diagnosis standard, screening the subjects according to the standard, dividing the subjects into an experimental group and a control group according to a random number table method, and ensuring that the two groups have no statistical difference in baseline data such as age, disease course and disease severity. The beneficial effects of the invention are that specific acupoints are selected for application according to the acupoint theory. Graphene is used as a novel nano material, has excellent thermal conductivity, electrical conductivity and biocompatibility, and can quickly transfer energy to deep acupoints, promote qi and blood circulation and relieve dysmenorrhea symptoms. Meanwhile, specific acupuncture points such as Shenque, Guanyuan, Sanyinjiao and the like are selected for application, and deep understanding and understanding of etiology and pathogenesis of primary dysmenorrhea are achieved based on the theory of traditional Chinese medicine.

The application belongs to the technical field of microorganisms, and provides a Streptococcus salivarius subsp. thermophilus HX-ST36, which has a preservation number of CGMCC NO. 32955. The Streptococcus salivarius subsp. thermophilus provided by the application can ferment Astragalus membranaceus Bunge-Angelica sinensis. In a cold coagulation and blood stasis type primary dysmenorrhea model mouse, the fermentation product thereof can significantly inhibit the writhing response, and can realize the effects of relieving dysmenorrhea and tonifying blood by reducing the levels of prostaglandin PGF2 and vasopressin ADH in uterine tissue and increasing the hemoglobin (HGB) content. The effect of the fermentation product in removing dampness is verified by improving the dampness signs of a crowd. The application further provides a probiotic preparation and a preparation method of a probiotic fermentation product. The preparation method of the probiotic preparation and the probiotic fermentation product is simple and can be produced on a large scale.

The invention provides a traditional Chinese medicine compound preparation for treating primary menstrual dysmenorrhea of women and a use method thereof. The traditional Chinese medicine compound preparation comprises a traditional Chinese medicine composition A and an external gel B adopting an extract of the traditional Chinese medicine composition A as an active ingredient, the traditional Chinese medicine composition A is prepared from the following traditional Chinese medicine raw materials in parts by weight: 15-30 parts of vinegar-processed rhizoma corydalis, 10-15 parts of angelica sinensis, 12-18 parts of salted eucommia ulmoides, 9-12 parts of roses, 6-9 parts of wine-processed radix paeoniae alba and 3-6 parts of ginger charcoal; the traditional Chinese medicine composition has the advantages of taking qi stagnation, blood stasis and kidney-yang deficiency composite pathogenesis synergistic treatment into consideration, avoiding the potential safety hazard of toxic medicinal materials, and realizing the dual effects of quickly relieving pain and periodically conditioning.

The invention discloses a traditional Chinese medicine composition for treating in-situ dysmenorrhea, which takes trogopterus dung as a monarch drug, mainly promotes blood circulation to remove blood stasis so as to achieve the effects of no pain and anti-inflammation, and can obviously reduce the content of prostaglandin E in inflammatory tissues so as to relieve or relieve pain caused by spasmodic contraction of uterus. Sappan wood and radix paeoniae alba are used as ministerial drugs, so that the monarch drugs are helped to strengthen blood circulation activation, stasis removal and pain relieving, warm channels, dispel cold, enrich blood, dissipate stasis and improve vasospastic contraction; the dragon's blood assists the monarch and ministerial drugs to stop bleeding and relieve pain, the brown sugar replenishes blood and disperses blood stasis, the radix achyranthis bidentatae assists the radix paeoniae alba to tonify the liver and kidney, nourish and nourish the liver, remove blood stasis and remove stagnation, and the liquorice serves as a conductant drug to harmonize all the drugs. The traditional Chinese medicine composition is reasonable in compatibility of medicines, and has the effects of promoting blood circulation to remove blood stasis, removing blood stasis, easing pain, enriching blood, regulating menstruation, reducing prostaglandin, improving blood viscosity of a matrix and uterine blood stasis and promoting menstruation in uterus so as to achieve the effect of no pain if the menstruation is unobstructed, so that primary dysmenorrhea is effectively treated.

This invention belongs to the field of microbial technology, specifically relating to a strain of *Lactobacillus rhamnosus* ZJUIDS07 with the effect of relieving primary dysmenorrhea and its application. This invention discloses a strain of *Lactobacillus rhamnosus* (ZJUIDS07). Lactobacillus rhamnosus The application of ZJUIDS07 in the preparation of drugs with the effect of relieving primary dysmenorrhea, Lactobacillus rhamnosus ( Lactobacillus rhamnosus The accession number for ZJUIDS07 is: CGMCCNO.23996. *Lactobacillus rhamnosus* ( Lactobacillus rhamnosus ZJUIDS07 regulates PGE2 and PGF 2a This level of fluid can help relieve menstrual cramps.

The invention relates to the technical field of traditional Chinese medicine compositions, in particular to a traditional Chinese medicine composition for improving lung deficiency type primary dysmenorrhea and a preparation method and application thereof. The traditional Chinese medicine composition specifically comprises multiple traditional Chinese medicine components including rhizoma polygonati, Chinese yam, rhizoma smilacis glabrae, radix salviae miltiorrhizae, yam rhizome, codonopsis pilosula, radix ophiopogonis from Sichuan of China, exocarpium citri grandis, spina date seeds, radix bupleuri, radix clematidis, morinda officinalis, rhodiola rosea, peach kernels, caulis spatholobi, southern apricot kernels and prepared rehmannia roots. The composition effectively relieves pain and improves accompanying symptoms through multi-target synergistic effects of promoting blood circulation to remove blood stasis, reinforcing qi and nourishing lung, warming kidney and strengthening spleen, regulating qi and relieving pain and the like. The formula follows the monarch, minister, assistant and guide theory of traditional Chinese medicine, is rigorous in compatibility, can comprehensively regulate qi, blood and viscera functions of a patient, is especially suitable for dysmenorrhea caused by deficiency of lung qi and unsmooth qi and blood circulation, and shows the advantages of overall treatment of traditional Chinese medicine. The preparation method is stable in process and easy to implement, and a safe and effective new choice for traditional Chinese medicine treatment is provided for lung deficiency type primary dysmenorrhea.

The invention discloses a method for evaluating the analgesic effect of angelica sinensis stomachache dropping pills, belongs to the technical field of efficacy evaluation, and aims to solve the problem that no scientific method is provided for efficacy verification of dysmenorrhea traditional Chinese medicine preparations in the formula of angelica sinensis. The method comprises the steps of experimental mouse selection, acetic acid body twisting model test, hot plate pain model test and xylene inflammation model test. Selecting an experimental rat, and testing the primary dysmenorrhea model constructed by estradiol and oxytocin. The method comprises the following steps: constructing an analgesic effect evaluation model, and verifying analgesic and anti-inflammatory effects of angelica sinensis stomachache relieving; pharmacodynamic mechanism analysis is further carried out on the angelica sinensis stomachache dropping pill for simulating primary dysmenorrhea, it is revealed that the content of serum E2, IL-6, PROG, beta-EP and TNF-alpha of primary dysmenorrhea model rats treated by the angelica sinensis stomachache dropping pill has an adjusting effect, the effect of relieving dysmenorrhea can be achieved, the inflammation effect can be relieved by inhibiting NF-kappa B and MAPK channels, and the pain behavior can be relieved.

The invention relates to the technical field of microorganisms, and particularly discloses lactobacillus acidophilus PIS4 capable of improving dysmenorrhea and application of the lactobacillus acidophilus PIS4. The lactobacillus acidophilus PIS4 is separated from genital tracts of healthy women, and is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.34554, and the preservation date is May 15, 2025. The lactobacillus acidophilus PIS4 is used for preparing the lactobacillus acidophilus PIS4. The lactobacillus acidophilus strain PIS4 provided by the invention is proved to be capable of inhibiting the gene expression of prostaglandin E synthase 2 (pges-2) and reducing the synthesis of prostaglandin E2 and prostaglandin F2alpha, and has effective relieving and improving effects on primary dysmenorrhea.

The invention relates to a preparation method of a stable rat model for primary dysmenorrhea with syndrome of cold coagulation and blood stasis, belongs to the technical field of animal model construction, and particularly relates to a construction method of a rat model for primary dysmenorrhea according with the pathogenesis characteristics of cold coagulation and blood stasis in traditional Chinese medicine. The model simulates the pathological process of cold evil invasion and uterus stasis and combines a modern medical hormone intervention means to realize the construction of a'disease and syndrome combined 'animal model, and is mainly used for the anti-dysmenorrhea mechanism research and drug screening of traditional Chinese medicine compound, acupuncture and other therapies. The adopted preparation method comprises the steps that 0.4 mg of estradiol benzoate is subcutaneously injected to each rat every day for 12 days, the injection dosage of the first day and the injection dosage of the last day are increased to 2 times, the rats are placed in the environment of-20 DEG C after injection is completed, epinephrine is injected twice every day from the 6th day of molding, and 2 U of oxytocin is injected to each rat intraperitoneal cavity 24 hours after the epinephrine is injected for the last time. The animal model obtained through the method is stable in phenotype and good in repeatability, compared with pure cold or pure irritation, more remarkable pain behaviors and biochemical changes can be obtained in a short period through combination with epinephrine, and pharmacodynamic screening is facilitated.

The present invention belongs to the field of traditional Chinese medicine, and specifically relates to a traditional Chinese medicine composition for treating primary dysmenorrhea, a cabu plaster thereof, and a preparation method. The traditional Chinese medicine composition for treating primary dysmenorrhea comprises vinegar-soaked Corydalis yanhusuo, salvia miltiorrhiza, cinnamon bark, Chuanxiong rhizome, vinegar-soaked Cyperus rotundus, Evodia rutaecarpa, and ginger. By preparing the above-mentioned traditional Chinese medicine composition into a cabu plaster for acupoint application on patients with primary dysmenorrhea, the preparation exhibits excellent quality, controllable quality, good in vitro transdermal absorption, and a particularly significant analgesic effect.

The invention belongs to the technical field of traditional Chinese medicine application, and particularly relates to a state-target combined dysmenorrhea square patch. The traditional Chinese medicine is prepared from the following medicines in parts by weight: 8-12 parts of cinnamon, 10-14 parts of baked ginger, 8-12 parts of angelica sinensis, 8-12 parts of ligusticum wallichii, 8-12 parts of radix paeoniae rubra, 8-12 parts of peach kernels, 8-12 parts of rhizoma cyperi, 10-14 parts of trogopterus dung, 10-14 parts of pollen typhae, 10-14 parts of rhizoma corydalis, 2-4 parts of amber, 8-12 parts of fennel, 18-22 parts of lindera aggregata and 13-17 parts of motherwort. The state-target combined dysmenorrhea plaster provided by the invention can improve the uterus microcirculation of a patient with primary dysmenorrhea and regulate the inflammation state, can significantly improve the severity of the patient with primary dysmenorrhea, and can reduce the severity and duration of dysmenorrhea symptoms.

This invention relates to the field of traditional Chinese medicine (TCM) technology, and more particularly to a TCM composition and pharmaceutical preparation for treating dysmenorrhea. The TCM composition includes Astragalus membranaceus, Codonopsis pilosula, stir-fried Atractylodes macrocephala, Angelica sinensis, Paeonia lactiflora, Cinnamomum cassia, Zingiber officinale (dried), Cyperus rotundus (processed with vinegar), Corydalis yanhusuo, and Glycyrrhiza uralensis (raw). The TCM composition provided by this invention has a simple formulation, addresses both the symptoms and the root cause, has a definite curative effect, and is highly safe. Using the TCM composition and pharmaceutical preparation provided by this invention to treat primary dysmenorrhea caused by cold stagnation and blood stasis, and qi and blood deficiency, it has a synergistic effect of "warming the meridians and dispelling cold, tonifying qi and nourishing blood, and promoting blood circulation and relieving pain."

This invention belongs to the field of biomedicine, specifically relating to the use of gemmaconazole in the preparation of drugs for treating primary dysmenorrhea. Gemarazole of this invention can significantly reduce the release of prostaglandins in uterine tissue, thereby relieving uterine smooth muscle spasms, reducing endometrial congestion and inflammatory responses, and thus alleviating pain, thus playing a role in treating primary dysmenorrhea. This provides a new option for clinical treatment of primary dysmenorrhea and has good application prospects.

The application discloses a uterus rhythmic contraction organ chip simulating primary dysmenorrhea and a preparation method thereof, and belongs to the technical field of organ chips. The uterus rhythmic contraction organ chip comprises, from top to bottom, an upper chamber layer, a middle porous membrane and a lower chamber layer. The upper chamber layer is internally provided with five parallel microchannels, including a first channel, a second channel and a third channel arranged in sequence in the middle, and a fourth channel and a fifth channel arranged on both sides. The adjacent microchannels are separated by microcolumns arranged at intervals, and the gap between the adjacent microcolumns constitutes a microenvironment interaction path between the microchannels. The bottom of the third channel penetrates through the upper chamber layer to form an open bottom end. The lower chamber layer is internally provided with an airway chamber located directly below the third channel. The middle porous membrane blocks the open bottom end of the third channel and physically isolates the airway chamber from the third channel.

The application discloses a ginger and jujube group formula, a preparation method thereof and application of the ginger and jujube group formula in prevention and treatment of cold syndrome primary dysmenorrhea products. The ginger and jujube group formula is composed of ginger, jujube, white peony root and fried licorice in a specific ratio, and an extract can be prepared by using a water extraction, alcohol extraction or water extraction and alcohol precipitation process. Experiments prove that the group formula and the extract can improve the cold and blood stasis signs of a cold syndrome primary dysmenorrhea model mouse, reduce writhing responses, reduce uterine and ovarian pathological injuries, down-regulate serum and uterine tissue inflammatory factor levels, and regulate endocrine and microcirculation disorders. The application has the advantages of scientific compatibility, definite curative effect, stable preparation process, high safety, suitability for long-term conditioning and the like, and is used for preventing and treating cold evil invasion and cold and blood stasis type primary dysmenorrhea.

An analgesic moxibustion stick for treating primary dysmenorrhea caused by cold stagnation and blood stasis effectively solves the problem of preparing medication for this type of dysmenorrhea. The analgesic moxibustion stick consists of a medicinal core and a paper roll. The medicinal core is made by mixing a compound powder of traditional Chinese medicine with moxa wool and stirring thoroughly. The compound powder of traditional Chinese medicine is composed of warming and dispersing cold herbs, blood-activating and stasis-removing herbs, meridian-guiding herbs, and transdermal adjuvants. The warming and dispersing cold herbs are made by mixing cinnamon, dried ginger, and evodia rutaecarpa powder; the blood-activating and stasis-removing herbs are made by mixing angelica sinensis, chuanxiong rhizome, red peony root, and peach kernel powder; the meridian-guiding herbs are made by mixing asarum and cyperus rhizome powder; and the transdermal adjuvant is borneol. The paper roll is made of double-layered mulberry bark paper rolled using conventional methods. The medicinal core is then placed inside the paper roll. This invention uses abundant raw materials, has a simple preparation method, is easy to produce, and has a scientifically sound drug formulation. It has the effects of warming yang and dispersing cold, activating blood and unblocking collaterals, and has good efficacy, making it valuable for practical application.

The invention provides a pharmaceutical composition for treating teenager dysmenorrhea and a preparation method and application thereof, the pharmaceutical composition is prepared by compatibility of 23 traditional Chinese medicines such as cassia twig, white peony root, pollen typhae, trogopterus dung, dragon's blood and the like, and a composite regulatory peptide and a micro-ecological regulator are innovatively added. Experiments show that the composition significantly inhibits a COX-2 / PGE2 pathway and reduces the level of inflammatory factors such as IL-6 and TNF-alpha through a multi-target synergistic effect, and in an animal model, the twisting frequency is reduced by 66%, the incubation period is prolonged by 142%, the COX-2 expression is reduced by 63%, and the abundance of intestinal bifidobacterium is improved by 20%. Clinical data show that the cure rate in three menstrual cycles reaches 85%, the recurrence rate is only 5%, and the traditional Chinese medicine composition is superior to the traditional western medicine. The preparation method adopts a process of combining gradient temperature decoction with 4-6 DEG C low-temperature active ingredient addition. The ointment formulation solves the problems of poor taste and low compliance of decoction, and has no adverse effect. Traditional dysmenorrhea treatment limitation is broken through, and a safe and efficient treatment scheme is provided for primary dysmenorrhea of teenagers through a synergistic interaction mechanism of the medicines.

According to the composition with the effects of replenishing blood, clearing damp and dispelling cold and the preparation method thereof, hemoglobin peptide, fish collagen peptide, donkey-hide gelatin peptide and iron are utilized to form composite micelles, then vitamin C-rich Chinese wolfberry powder is utilized to be mixed, a mixture of lily and poria cocos powder is fermented by casei paracasei ID01 to serve as a wrapping network system, and the composition is prepared through the steps of preparing the composition with the effects of replenishing blood, clearing damp and dispelling cold. Meanwhile, a cross-linking agent is added for cross-linking, so that a polypeptide-iron-vitamin C-fermentation compound quaternary complex structure is formed. A slow-release system is evaluated by simulating a static digestive tract, so that targeted delivery and absorption optimization of iron elements are realized. In a cold coagulation and blood stasis type primary dysmenorrhea model mouse, the composition can significantly inhibit the writhing reaction, and can achieve the effects of enriching blood, clearing damp and dispelling cold by reducing the levels of prostaglandin PGF2 and vasopressin ADH in uterine tissues and increasing the content of hemoglobin HGB.

This invention provides a composition with menstrual regulating and blood-activating effects and its application in the preparation of external products, relating to the field of biomedical technology. This invention utilizes a composition composed of Chinese herbal raw materials including *Cephalotaxus fortunei* bark, star anise, *Erigeron breviscapus*, *Piper longum*, *Caulis thunbergii*, *Radix Achyranthis Bidentatae*, *Radix Scutellariae*, *Radix Achyranthis Bidentatae*, *Radix Panax notoginseng*, *Radix Bambusae in Taeniam*, and *Radix Achyranthis Bidentatae* to prepare a traditional Chinese medicine acupoint patch for treating or improving primary dysmenorrhea. The herbal acupoint patch can inhibit the expression of inflammatory factors, downregulate the expression level of COX-2, thereby inhibiting PGF2α, increasing the level of PGE2, and reducing uterine tissue inflammation, achieving multi-target treatment of primary dysmenorrhea. The herbal acupoint patch utilizes the power of the medicine through the acupoint, and the acupoint utilizes the properties of the medicine. The meridians are the channels, and the acupoints are the gateways. The medicinal power directly reaches the Chong and Ren meridians and the uterus, so that cold is warmed and dispelled, blood stasis is cleared and eliminated, and qi and blood flow smoothly, thus curing dysmenorrhea. It plays a role in warming the meridians and dispelling cold, promoting qi and blood circulation, removing blood stasis and relieving pain, providing a new direction for the external treatment strategy of traditional Chinese medicine for primary dysmenorrhea.

The application discloses a deer source extract and application thereof in preparation of a medicine for treating primary dysmenorrhea, and belongs to the technical field of medicines. The deer horn extract is prepared from deer horn through alkali extraction and acid precipitation, and is purified through molecular weight cut-off, and four P1-P4 deer horn polypeptides with specific amino acid sequences are obtained through further multi-dimensional activity screening. The deer horn polypeptide can play a synergistic effect on multiple targets through regulating uterine tissue prostaglandin balance, inhibiting abnormal contraction of uterine smooth muscle, reducing local inflammatory response, improving ischemia and hypoxia injury, and regulating pain signal transmission, and effectively improves symptoms such as spasm and pain of primary dysmenorrhea. The application also prepares various dosage forms such as tablets and capsules by taking the deer horn extract or the deer horn polypeptide as a main active ingredient, and can meet the needs of different scenes such as acute symptom relief and long-term periodic conditioning, has high safety, good patient compliance, and provides a safe and effective natural intervention scheme for primary dysmenorrhea.

The invention relates to the technical field of traditional Chinese medicines, in particular to application of a traditional Chinese medicine composition in preparation of a medicine for preventing and treating dysmenorrhea. The invention provides application of a traditional Chinese medicine composition in preparation of a medicine for preventing and treating dysmenorrhea. The traditional Chinese medicine composition comprises pseudo-ginseng, ligusticum wallichii, corydalis tuber, radix paeoniae alba, radix achyranthis bidentatae, rhizoma cibotii, cooked rhubarb and radix angelicae pubescentis. The traditional Chinese medicine composition can remarkably reduce the serum E2 level of rats with primary dysmenorrhea, increase the serum PROG level and improve the plasma beta-EP level; and the contents of serum luteinizing hormone (LH) and follicle stimulating hormone (FSH) of a rat with cold coagulation and blood stasis type dysmenorrhea are remarkably reduced. And the preparation method of the compound combination medicine for treating dysmenorrhea is simple, rapid, efficient and easy to operate. The prepared capsules, tablets, granules, pills, injections and the like are convenient to carry and take.

The invention discloses a traditional Chinese medicine composition for treating qi deficiency and blood stasis type primary dysmenorrhea. The traditional Chinese medicine composition comprises the following raw materials in parts by weight: 40-50 parts of codonopsis pilosula, 10-20 parts of dogwood, 30-40 parts of Chinese yam, 35-45 parts of rhizoma polygonati, 30-40 parts of angelica sinensis, 30-40 parts of fructus lycii, 35-45 parts of mulberry, 20-30 parts of raspberry, 30-40 parts of poria cocos, 30-40 parts of liquorice, 20-30 parts of fructus amomi, 30-35 parts of endothelium corneum gigeriae galli, 10-15 parts of rosa rugosa plena, 20-30 parts of pericarpium citri reticulatae and 40-50 parts of dendrobium officinale. When the prescription is used for treating qi deficiency and blood stasis type primary dysmenorrhea, qi and blood are treated simultaneously, and codonopsis pilosula, American ginseng, angelica sinensis, colla corii asini and longan aril are used for tonifying qi and blood.