606 results about "Pathogenesis" patented technology

The invention relates to the technical field of artificial intelligence medical diagnosis, and discloses a remote disease diagnosis system based on artificial intelligence driving. The system comprises five functional modules, a real-time medical data acquisition module acquires multi-modal physiological data of a patient from a remote terminal in real time, and the multi-modal physiological data comprises vital sign time sequence data, medical image data and symptom description text data; the cross-modal correlation analysis module carries out feature fusion on the data, extracts a correlation mode between modals and generates an abnormal feature data set with a weight mark; establishing a dynamic diagnosis baseline by the dynamic diagnosis baseline model, and adapting to individual physiological feature changes by updating parameters; the abnormal behavior detection module is used for comparing the real-time data with a baseline, identifying abnormal indexes and generating a marking result; the deep pathogenesis tracing module analyzes a potential disease mode and traces a pathogenesis path in combination with a patient's historical medical record. The system can optimize the remote diagnosis effect and assist clinical diagnosis and treatment.

The invention provides an application of WNT7B in construction of a myopia animal model. Homologous genes wnt7ba and wnt7bb of the WNT7B in the zebra fish are knocked out or knocked down by applying a CRISPR / Cas9 gene editing technology, an animal model of which the eye axis length is remarkably increased, the eyeball movement frequency is remarkably reduced and the light response is reduced is obtained, and the result shows that the WNT7B is related to the high myopia. The model provides a favorable tool for pathogenesis and drug screening of high myopia, and has a good application prospect.

The invention belongs to the field of immunological detection, and provides a double-antibody sandwich ELISA kit for detecting the content of human beta-NGF. The enzyme-linked immunosorbent assay kit constructed by the invention is based on a double-antibody sandwich method, and is combined with a biotin-streptavidin signal amplification system and an enzyme developing amplification system, so that the detection sensitivity and specificity are greatly improved, and low-content samples can be accurately and quickly detected. The kit shows excellent stability and specificity and can be used for quantitatively detecting the content of beta-NGF in samples such as human serum, cell supernatant or tissue homogenate, so that an objective detection basis is provided for diagnosis, curative effect evaluation and pathogenesis research of related diseases.

The invention discloses application of docosapentaenoic acid or medicinal salt thereof in preparation of a medicine for preventing and / or treating systemic lupus erythematosus. The invention aims to provide novel application of docosapentaenoic acid in treatment of systemic lupus erythematosus. In view of various immune abnormalities existing in the systemic lupus erythematosus morbidity process, the docosapentaenoic acid has potential application value in the field of systemic lupus erythematosus treatment due to the effects of regulating immune cell functions and improving immune microenvironment.

The invention belongs to the technical field of brain function monitoring, and discloses a three-in-one non-invasive brain function monitoring system and method, a medium, equipment and application, and the monitoring method comprises the following steps: monitoring brain oxygen saturation of a critical patient; carrying out transcranial Doppler ultrasonic measurement on intracranial blood vessel blood flow velocity and ultrasonic measurement on the inner diameter of the optic nerve sheath; monitoring a quantitative electroencephalogram of the patient; various brain function monitoring series indexes and continuous changes of the indexes along with disease evolution are obtained, the pathogenic mechanism and pathogenesis of the indexes are found in time, and primary diseases and related complications of the primary diseases are treated. According to the invention, the brain oxygen saturation can avoid too low brain oxygen or low brain oxygen for a long time and too high brain oxygen due to oxygen utilization disorder; cerebral blood flow can be monitored through a transcranial Doppler (TCD) technology to find an optimal craniocerebral perfusion pressure (CPP), and a cerebral blood flow self-adjusting function is enabled to be in an optimal state; the electroencephalogram can avoid early warning of over-sedation or abnormal discharge and the like. Through organic combination of the three, noninvasive, real-time and dynamic monitoring of critical patients is realized.

The invention provides an intelligent symptom machine distinguishing and treating system based on four diagnostic methods of traditional Chinese medicine. The system comprises a four diagnostic method information acquisition module used for acquiring multi-modal data; the multi-modal data processing module is used for preprocessing the multi-modal data; the feature fusion module is used for carrying out feature fusion on the preprocessed data, and the domain-specific reasoning core module is used for carrying out traditional Chinese medicine pathogenesis dialectical reasoning on fused features based on a thinking chain technology and outputting structured results including dialectical analysis, syndrome types, treatment rules and prescriptions; the traditional Chinese medicine knowledge graph enhancement module is used for performing verification, constraint and knowledge enhancement on the structured result and outputting a second structured result; the interpretability output module is used for outputting a diagnosis and treatment report according to the second structured result, and displaying the diagnosis and treatment report through a graphical user interface. The application utilizes a domain-specific large language model to deeply understand complex association among symptoms and infer pathogenesis behind, and generates a complete syndrome differentiation and treatment scheme conforming to traditional Chinese medicine logic; the intelligent degree is high, and reliability guarantee is provided for clinical application through integration of the traditional Chinese medicine knowledge graph.

The invention belongs to the field of prevention and treatment of tilletia controversa Kuhn, and particularly relates to a tilletia controversa Kuhn effect protein g2339 and application thereof. An amino acid sequence of the Tilletia controversa Kuhn effect protein is shown as SEQ ID NO: 2. The effect protein plays an important role in the process of inhibiting plant defense reaction, and has an interaction relationship with two wheat proteins in the process of infecting wheat by Tilletia controversa Kuhn. According to the invention, the pathogenesis of Tilletia controversa Kuhn can be deeply researched, and the cultivation of the wheat variety resistant to Tilletia controversa Kuhn can be promoted.

The invention discloses a method for screening a Wilson disease serum exosome miRNA biomarker based on a transcriptomics technology, and belongs to the field of bioinformatics analysis of diseases. According to the invention, the effect of miRNA in WD pathogenesis is discussed by identifying serum exosome miRNA, and a potential biomarker is provided for accurate diagnosis and treatment of the disease. According to the invention, 59 DE-miRNAs are identified by analyzing the expression of differential miRNAs of WD and control group patients, and the DE-miRNAs are related to key approaches such as metabolic regulation, cancer progression, signal transduction and the like. Besides, the reliability of the key DE-miRNA, including miR-451a, miR-204-5p and the like, is confirmed through experimental verification, and the potential of the key DE-miRNA as a non-invasive biomarker is indicated. Research results provide important theoretical basis and new insight for accurate diagnosis of WD and development of potential therapeutic targets.

The invention relates to the technical field of biological medicines, and provides application of NR1D1 in treating sicca syndrome, related medicines and the like. The scheme of the invention discovers and proves that the NR1D1 gene participates in the sicca syndrome pathogenesis process, can significantly influence the function of submaxillary gland cells, and is an effective sicca syndrome immune inflammation regulation diagnosis and treatment target.

The invention discloses a preparation method of a medicinal and edible traditional Chinese medicine composition for preventing and treating pregnancy complications. The medicinal and edible traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 24 parts of radix rehmanniae, 12 parts of fructus gardeniae and 9 parts of folium mori. The composition embodies the essence of compatibility of monarch, minister, assistant and guide of a traditional Chinese medicine compound, the radix rehmanniae is used as a monarch drug, the fructus gardeniae is used as a minister drug, the folium mori is used as an assistant drug, and the composition is mainly characterized by nourishing the kidney, containing wood, clearing away fire, calming the liver and lightly dredging upper jiao and exactly conforms to the core pathogenesis of yin deficiency and yang hyperactivity of gestational hypertension. Modern pharmacological experiments prove that the three traditional Chinese medicinal materials all have definite antihypertensive activity, and the action mechanism shows the characteristic of multi-target synergism. The raw materials in the formula are homologous in medicine and food, free of toxic and side effects, mild in medicine property and capable of considering fetal elements, the three medicines all belong to clinical common miscarriage prevention or miscarriage prevention categories, worries about blood breaking and fetal movement are avoided, and the traditional Chinese medicine is suitable for being used in the pregnancy stage. The pharmaceutical composition disclosed by the invention can be used for relieving clinical symptoms of hypertensive patients in pregnancy and improving the outcome of bad pregnancy.

The invention discloses a construction method and application of a preeclampsia animal model based on an RASA3 target, and belongs to the technical field of biomedicine.The preeclampsia animal model is constructed by injecting Ad-RASA3 into a pregnant mouse and monitoring pathological indexes, related to preeclampsia, of the pregnant mouse. According to the method, the animal model with preeclampsia-like clinical symptoms can be stably induced, the abnormal change of the uterine spiral artery remodeling process can be systematically monitored, the method is high in operation controllability and model stability, and an effective tool can be provided for deeply researching the pathogenesis of preeclampsia and developing a novel diagnosis and treatment strategy.

The invention belongs to the technical field of prevention and treatment of rice blast, and particularly relates to a polypeptide MP1 for inhibiting rice blast bacteria and application thereof. The invention discloses a polypeptide MP1 for inhibiting magnaporthe oryzae. The amino acid sequence of the polypeptide MP1 is as shown in SEQ ID NO. 1. The polypeptide MP1 has an obvious inhibition effect on the magnaporthe oryzae appressorium, and the EC50 formed by the MP1 polypeptide on the appressorium is 134.60 mu M. Experiments show that the polypeptide can effectively interfere the normal differentiation and development of the appressorium in the key pathogenic stage of magnaporthe oryzae, so that the polypeptide has a strong prevention and control potential in the early stage of pathogenic bacterium infection. The polypeptide MP1 provided by the invention can significantly reduce the pathogenicity of magnaporthe oryzae to barley and rice, and provides an experimental basis for developing a novel biopesticide for preventing and treating the magnaporthe oryzae.

The invention belongs to the technical field of medical diagnosis, and provides an HIV (human immunodeficiency virus) combined non-alcoholic fatty liver diagnosis and prediction marker and application. The marker comprises 39 biomarkers in total, wherein the 39 biomarkers comprise two intestinal microorganisms of enterobacter and klebsiella and 37 plasma metabolites of diacylglycerol, lysophosphatidic acid and the like; by integrating microbiome and metabonomics, the multi-dimensional marker is screened and verified, so that the problems of lack of high specificity and sensitivity, difficulty in early disease screening and incapability of effective risk assessment of an HIV (Human Immunodeficiency Virus) combined NAFLD (Non-Amplified Fragment Leukemia) early diagnosis method caused by unknown pathogenesis in the prior art are solved; the marker combination is applicable to preparation of diagnostic kits, construction of disease prediction models and development of targeted therapy drugs, and a theoretical basis is provided for early discovery, risk early warning and advanced intervention of the HIV combined with the non-alcoholic fatty liver disease.

The invention relates to the technical field of agricultural biology, in particular to a verticillium dahliae heat shock protein 90 ATP enzyme activating factor VdAHA1 as well as a coding gene and application of the verticillium dahliae heat shock protein 90 ATP enzyme activating factor VdAHA1. The invention provides a verticillium dahliae heat shock protein 90 ATP enzyme activating factor VdAHA1 related to high temperature resistance, drug resistance, environmental adaptability, ergosterol synthesis capability and pathogenicity of verticillium dahliae and a coding gene of the VdAHA1, and the VdAHA1 gene positively regulates the pathogenicity of verticillium dahliae and can be used as an important candidate gene for pathogenesis research of verticillium dahliae. The invention provides a theoretical basis for further understanding the pathogenic mechanism of pathogenic bacteria, also provides a new gene resource for verticillium dahliae pathogenic related genes, also provides a new idea for prevention and control of cotton verticillium wilt, and has a wide application prospect.

The invention belongs to the field of biological immunity, and discloses a linear B cell epitope of African swine fever virus NP419L protein and application. The epitope sequence shows good immunoreactivity through ASFV positive pig serum recognition verification. The African swine fever positive serum can be identified by using the B cell epitope identified by the invention to carry out ELISA (Enzyme-Linked Immunosorbent Assay) experiment, and the sensitivity of the African swine fever positive serum is superior to that of NP419L protein full length and other truncated fragments. The epitope identified by the invention has high conservative property in reference ASFV strains in a plurality of popular regions in China, and has wide universality and adaptability. The kit can be widely applied to etiological diagnosis, serological diagnosis, immunological detection and disease prevention and treatment of African swine fever viruses, and can be applied to preparation of African swine fever vaccines, pathogenesis research and the like.

The invention discloses an application of a Gnai2 protein inhibitor in preparation of a medicine for treating diabetic nephropathy, and belongs to the technical field of biological medicine, research shows that expression of Gnai2 in a diabetic nephropathy sample is up-regulated and is in positive correlation with a renal injury index, after the Gnai2 gene is knocked out, lipophagy can be promoted, lipid droplet deposition can be reduced, development of diabetic nephropathy can be relieved, and the Gnai2 protein inhibitor can be used for treating diabetic nephropathy. Overexpression of the Gnai2 gene can significantly inhibit lipophagy, promote lipid droplet deposition and aggravate development of diabetic nephropathy. The Gnai2 protein as a drug target can be used for screening and discovering substances for preventing and treating the diabetic nephropathy, and the Gnai2 protein inhibitor has a good application prospect in the aspect of preparing drugs for treating the diabetic nephropathy. According to the invention, the blank of'lipid metabolism disorder 'research in the pathogenesis of diabetic nephropathy is filled, and the normal form transformation of diabetic nephropathy from single sugar control treatment to'lipid metabolism-inflammation / fibrosis' multi-channel precise treatment is promoted.

The invention belongs to the technical field of traditional Chinese medicine, and particularly relates to a traditional Chinese medicine decoction for treating vitiligo and a preparation method thereof.The traditional Chinese medicine decoction for treating vitiligo is prepared from ten medicinal materials including notopterygium root, divaricate saposhnikovia root and the like according to the core pathogenesis of vitiligo rheumatism stagnated heat syndrome, the brand new theory of treating both black and white diseases and both black and white diseases is creatively provided, and on the basis of the theory, the traditional Chinese medicine decoction for treating vitiligo is prepared into the traditional Chinese medicine decoction for treating vitiligo. White spots and surrounding deepened skin are brought into systematic conditioning for the first time, the formula is compatible with black and white medicine pairs, black is used for treating white, white is used for treating black, overall conditioning and two-way adjustment are achieved, the white spots can recover to the normal skin color, and due to the innovative application of the two groups of black and white medicine pairs (wine-processed rhizoma chuanxiong, radix angelicae sinensis, radix rehmanniae preparata and radix paeoniae alba) in the formula, black is used for treating white, white is used for treating black, and two-way adjustment is achieved. And meanwhile, the clinical piebald skin disease symptom is also eliminated, so that the white spots recover to the normal skin color.

The invention discloses an application of astaxanthin in preparation of a regulator for improving the gentisic acid level. On the basis of non-targeted metabonomics analysis, it is found for the first time that in the SAP pathogenesis process, the level of a flora metabolite, namely gentisic acid (GA), in intestinal contents is remarkably reduced, and in-vivo gentisic acid level can be remarkably callback through astaxanthin intervention. Further research proves that exogenous supplement of gentisic acid can effectively simulate the protection effect of astaxanthin, including improvement of pancreatic tissue pathological damage, reduction of serum lipase and amylase activity, and inhibition of systemic inflammatory response (down-regulation of IL-1beta, IL-6 and TNF-alpha and up-regulation of IL-10). The invention not only discloses a new mechanism that astaxanthin plays a therapeutic role through an'intestinal flora-metabolite 'axis, but also determines that gentisic acid is used as a novel drug target for treating SAP, and constructs a new method for screening drugs for treating SAP according to the novel drug target.

The invention belongs to the technical field of biological genetic engineering, and particularly relates to a mouse model construction scheme capable of reproducing a key phenotype of hemophagocytic syndrome, which comprises the following steps: inserting a loxP-STOP-loxP-LMP1 conditional expression cassette into a Rosa26 safe port site in a fixed point manner to obtain an Lmp1cKI mouse strain; and hybridizing the mouse with an Ncr1-Cre transgenic mouse of NK cell specific expression Cre recombinase. In a double transgenic offspring (Lmp1Ncr1-Cre), STOP fragments are cut off by Cre enzyme, and continuous high expression of EB virus LMP1 protein in NK cells is driven. The model does not need chemical induction or immune intervention, and typical hemophagocytic syndrome phenotypes, namely serum IL-6, TNF-alpha and IFN-gamma, spontaneously appear within 7-9 days after young rats are born; the body weight is reduced and the survival rate is obviously reduced; the proportion of lymphocytes is sharply reduced; and NK cells are depleted. The invention provides a stable and reliable tool for hemophagocytic syndrome pathogenesis research, drug screening and treatment evaluation.

The invention provides a traditional Chinese medicine for treating protrusion of lumbar intervertebral disc, and relates to the technical field of traditional Chinese medicines. Aiming at the core pathogenesis of qi stagnation and blood stasis, deficiency of liver and kidney and cold-dampness arthralgia of the lumbar intervertebral disc protrusion, a synergistic compatibility concept of promoting blood circulation to remove blood stasis, tonifying liver and kidney, dispelling cold-dampness and activating meridians to stop pain is adopted. The traditional Chinese medicine is prepared from 12 traditional Chinese medicines such as ligusticum wallichii, safflower, eucommia ulmoides and radix achyranthis bidentatae through an optimized process of crushing, extracting, concentrating and forming, and the dosage form comprises tablets, capsules or oral liquid. The core innovation lies in accurate matching of the formula, multi-target synergistic effect is achieved, and the problems that traditional Chinese medicine is slow in curative effect and poor in pertinence are solved. Clinical verifications show that the traditional Chinese medicine can relieve lumbar pain within 7 days and improve symptoms such as numbness of lower limbs and limited movement within 1-2 treatment courses, has the total effective rate of greater than or equal to 92%, is safe, has no obvious toxic or side effect, is suitable for patients with different types of lumbar disc herniation, and is convenient to take and high in cost performance.

The invention discloses a method for establishing a hemophagocytic syndrome in-vitro cell disease model, and belongs to the technical field of biology. Based on human PBMC construction, the reaction of a human immune system can be truly reflected, experimental result deviation caused by species difference in an animal model is avoided, and a more accurate model basis is provided for research of human diseases. In addition to T cell over-activation, the HLH model of the present application has a clear key feature of NK cell reduction, which is HLH, accompanied by up-regulation of cytokines such as interferon-gamma and IL-10. The establishment process of the model is rapid and efficient, the experimental period is remarkably shortened, a large number of samples or drugs can be rapidly screened, the research efficiency is improved, and the conversion process from basic research to clinical application is accelerated. The method can be used for researching various mechanisms related to the HLH, is beneficial to more comprehensive understanding of pathogenesis of the HLH, and provides a theoretical basis for developing preliminary screening of targeted drugs.

The invention relates to the technical field of organoid culture, in particular to a pulmonary fibrosis organoid model and a construction method and application thereof. The pulmonary fibrosis organ-like model is obtained by co-culturing lentivirus stably transfected alveolar epithelial cells, human embryonic lung fibroblasts MRC-5 and macrophages. According to the invention, macrophages are innovatively introduced, a ternary co-culture system of lentivirus stably transfected alveolar epithelial cells, human embryonic lung fibroblasts MRC-5 and macrophages is constructed, and a key'epithelial injury-immune infiltration-fibroblast activation 'pathological triangle in a pulmonary fibrosis pathogenesis process can be more comprehensively simulated; the finally obtained pulmonary fibrosis organ-like model provides an efficient tool for pulmonary fibrosis mechanism research and research, development and screening of anti-pulmonary fibrosis drugs.

The invention discloses a traditional Chinese medicine composition for treating recurrent urinary tract infection as well as a preparation method and application thereof. The traditional Chinese medicine composition is prepared from 30-90 parts of astragalus membranaceus, 3-9 parts of cordyceps mycelia, 12-36 parts of rhizoma anemarrhenae, 10-30 parts of golden cypress and 6-18 parts of cinnamon. According to the pathogenesis characteristics of recurrent urinary tract infection and the principle of treatment based on syndrome differentiation of traditional Chinese medicine, the optimal raw material composition and dosage part ratio are obtained through screening, simplification and optimization of a large number of experiments, and the traditional Chinese medicine composition can remarkably improve the frequent micturition symptom, bacterial load and bladder pathological injury of rUTI mice. In addition, the drug-containing serum can promote migration of the SV-HUC-1 cells and reduce the level of inflammatory factors secreted by co-culture of the SV-HUC-1 cells and the UTI89 cells; the adhesion and internalization of the UTI89 to the cells are reduced. In addition, according to different bacterial strains and mouse modeling results, it is found that the modeling rate is obviously increased in a mode of enhancing infection every 24 hours, a recurrent urinary tract infection model of UTI89 infected C57BL / 6J is constructed, and a stable model is provided for screening drugs for treating recurrent urinary tract infection.

The present application relates to a method for constructing a human acute liver injury and chemotaxis model based on a normal liver organoid and application thereof. Specifically, the present application provides a method for constructing a human liver organoid; further, contacting the liver organoid with a hepatotoxic drug can construct a human acute liver injury model; co-culturing the organoid or the injury model with immune cells can construct an in vitro immune cell chemotaxis model. The present application also provides reversible immunoaffinity magnetic beads for isolating VSIG4-positive macrophages and a method for isolating the same. The human liver organoid, acute liver injury model and chemotaxis model of the present application can be used for screening drugs for treating acute liver injury, evaluating the hepatotoxicity of compounds, researching the pathogenesis of acute liver injury and evaluating the effect of immunomodulatory drugs.

The invention belongs to the technical field of traditional Chinese medicine preparations, and particularly relates to Chinese herbal medicine emulsifiable paste for dermatitis and a preparation method thereof. The traditional Chinese medicine composition is prepared from the following raw materials: radix sophorae flavescentis, cortex phellodendri, cortex dictamni, fructus cnidii, fructus kochiae, radix scutellariae, radix paeoniae rubra, radix salviae miltiorrhizae, herba schizonepetae, radix saposhnikoviae Systematic design is carried out aiming at complex pathogenesis of seborrheic dermatitis, medicinal materials such as radix sophorae flavescentis and cortex phellodendri are used for synergistically clearing heat and drying dampness, malassezia is inhibited, and lipid control and bacteriostasis are carried out from the source; radix scutellariae, radix salviae miltiorrhizae and the like are combined to cool and activate blood and control inflammation for a long time; the herba schizonepetae, the radix saposhnikoviae and the like are added for targeted itching relieving and secondary injury reduction; in the dosage form, a low-oil-burden matrix composed of light grease such as jojoba oil and squalane is adopted, the texture is light and thin, hair follicles are not blocked, and phytosterol is introduced to synchronously repair the skin barrier; according to the system, the use comfort and compliance are remarkably improved while permeation of active ingredients is guaranteed.

The invention discloses a diabetic foot prevention system and method based on immersive experience and artificial intelligence, and relates to the technical field of disease prevention, and the diabetic foot prevention system comprises an immersive interaction module, a multi-sensory stimulation module, a physiological monitoring module, an artificial intelligence module and a disease intervention module. The interactive 3D virtual scene is constructed through the immersive interaction module, the pathological evolution process of the diabetic foot is visually displayed, the problem that disease development is difficult to truly present in a traditional science popularization mode is solved, a diabetic patient is helped to deeply understand the pathogenesis, the evolution stage and the potential consequence of the disease, and the development of the diabetic foot is promoted. The foot disease state of the diabetic foot patient is simulated through the multi-sensory stimulation module, so that the diabetic patient experiences real symptoms such as pain and burning feeling, the fluke mind of the diabetic patient is broken, the foot health is actively concerned, and the diabetic foot risk is improved. And the risk of delay of the intervention opportunity due to light diseases is reduced.

The invention provides a traditional Chinese medicine preparation for treating peripheral neuropathy caused by chemotherapy, the traditional Chinese medicine preparation comprises an external preparation and an internal preparation, and discloses a preparation method and application of the traditional Chinese medicine preparation. The core pathogenesis of qi and blood stasis and yang deficiency and cold coagulation of the peripheral neuropathy caused by chemotherapy and the characteristic treatment method of tonifying qi and activating blood and warming yang and dredging collaterals are innovatively provided; the composition is rigorous and rigorous, and is clinically safe and effective.

The application provides application of a traditional Chinese medicine composition in preparation of a heart failure resisting medicine. A first aspect of the application provides application of a traditional Chinese medicine composition in preparation of a heart failure resisting medicine, wherein the traditional Chinese medicine composition comprises the following raw materials in parts by mass: 165-185 parts of radix notoginseng, 90-110 parts of radix astragali, 10-30 parts of radix ginseng, and 10-35 parts of pachyrhizus or radix puerariae. According to the pathogenesis of heart failure, i.e. yin deficiency and less body fluid, the traditional Chinese medicine composition can improve dry mouth and throat, and thirst caused by heat, and finally realize the treatment of heart failure through prescription. Animal experiments show that the traditional Chinese medicine composition has a significant anti-heart failure effect, and can obviously improve heart failure of spontaneous hypertension rats, and has a significant curative effect.

The invention belongs to the technical field of medical experimental animal models, and particularly relates to a construction method of a rabbit ear acne model for studying acne pathogenesis and drug curative effect. A healthy New Zealand large-ear white rabbit is selected, a testosterone solution is subcutaneously injected to induce endocrine disorder, oleic acid is smeared at an opening of an external auditory canal on the inner side of an ear, dermatobacterium acnes suspension is subcutaneously injected, and whether acne-like lesions such as erythema, papule and comedo appear on the skin of the ear or not is observed. The method is easy and convenient to operate, low in cost, good in repeatability, capable of simulating the pathological process of human acne and suitable for acne pathogenesis research and drug screening.