45 results about "Mouse strain" patented technology

Peptide constructs comprised of multideterminant T helper peptides from the envelope glycoprotein of HIV previously identified to induce proliferative responses in four different haplotypes of mice and IL-2 responses in 52-73% of HIV positive, flu positive patients (cluster peptides), were co-linearly synthesized with the peptide 18 of the V3 loop of HIV-1 gp 160, corresponding to the principal neutralizing determinant of HIV-IIIB and also shown to contain a dominant CTL epitope. Cognate help for peptide 18 antibody was elicited following a single immunization in all strains of mice which had previously responded to a T cell epitope encompassed by the peptides. In two strains of mice, the level of neutralizing antibody achieved was comparable to levels adequate for protection from homologous viral challenge in chimpanzees. After a single boost, much higher antibody titers for 90% neutralization in the range of 1:1000 to 1:16,000 were achieved. Spleen cells from mice of three distinct MHC haplotypes sharing the Dd class I MHC molecule but with different class II molecules, immunized with the compound peptides, exhibited enhanced gp160-specific CTL activity.

The invention belongs to the field of genetic background identification and genetic pollution detection of inbred mouse strains, and relates to an SNP loci group for genetic quality monitoring of CBA / CaJ inbred mice, and primer combinations and application of the SNP loci group. The SNP loci include 10 specific recognition loci for a CBA strain, 70 recognition loci for strains other than CBA, and16 universal loci. The results of 96 SNP loci can accurately reflect the degree of coincidence of backgrounds of subject mouse individuals with specific inbred mouse strains, comprehensively detect the genetic traits and variation of the genome of the subject mouse individual, and realize the monitoring of mouse genetic quality status and quality of experimental stability. Reaction conditions areoptimized for the SNP primers in the subsequent detection to ensure that the detection is sensitive, specific and efficient. In addition, multiple sets of primers are designed for 10 specific loci tofacilitate selection according to different detection requirements and specific detection conditions.

The invention relates to the development of an animal model for testing various agents in the treatment of a clotting disorder. More specifically, the invention relates to the use of ultra-large molecular weight multimers of von Willebrand factor (VWF) in various mouse strains to induce thrombotic thrombocytopenic purpura (TTP)-like symptoms for the development of a mouse model of TTP. The invention also provides methods for generating such animal disease models and screening methods for identifying biologically active compounds which are effective in the treatment of TTP.

The invention discloses a mouse cryopreserved sperm resuscitation liquid compound and a preparation method and application thereof. The compound comprises a basis solution, methyl B cyclodextrin MBCDand polyvinyl alcohol PVA. The preparation method comprises the steps that firstly, methyl B cyclodextrin MBCD and polyvinyl alcohol PVA are dissolved in the basic solution, after sufficient dissolution, the mixture is filtered through a bacterium filter device, and mouse cryopreserved sperm resuscitation liquid is obtained. According to the compound, methyl B cyclodextrin MBCD and polyvinyl alcohol PVA are added to the sperm resuscitation liquid, the sperm capacitation process is promoted, the capacitation of sperms is improved, the number of the sperms with fertilization capacity is increased, the fertilization probability of the sperms and eggs is increased, and the fertilization rate is increased. The compound is beneficial to successful preservation of mouse sperm resources, and reestablishment of mouse strain resources is increased and accelerated.

A transgenic animal model for evaluating growth, survival and / or metastasis of xenotransplanted normal or tumor cells or tissue is disclosed, in which a human growth factor, hHGF stimulates growth in vivo of human cells or tissue. A strain of Tg mice on the C3H background that is immunocompromised as a result of a homozygous scid gene has been bred which express a nucleic acid encoding hHGF / SE The ectopically expressed hHGF / SF ligand significantly enhances growth of human tumor cell lines and explanted tumor cells or tissue that express the Met receptor for hHGF. Such animals also have an enlarged normal livers and greater than normal liver regenerative capacity. Any Met-expressing hHGF-dependent human cells, including hepatocytes and various stem cells can survive and grow in such animals.

The invention relates to construction for a number 1 chromosome substitution laboratory mouse strain C57BL / 6-Chr1NZW. According to the construction for the number 1 chromosome substitution laboratory mouse strain C57BL / 6-Chr1NZW, a new chromosome substitution laboratory mouse strain is constructed. A genetic background of the chromosome substitution laboratory mouse strain is based on a C57BL / 6 mouse, while wherein a number 1 chromosome is from an NZW mouse. According to the construction for the number 1 chromosome substitution laboratory mouse strain C57BL / 6-Chr1NZW, the chromosome substitution laboratory mouse strain can be used for research of character differences brought by different number 1 chromosome genomes under the same genome background, and can be a useful tool for research of the chromosome genome functions by adoption of a genetic method.

The invention relates to construction for a number 1 chromosome substitution laboratory mouse strain C57BL / 6-Chr1C3H / HeJ. According to the construction for the number 1 chromosome substitution laboratory mouse strain C57BL / 6-Chr1C3H / HeJ, a new chromosome substitution laboratory mouse strain is constructed. A genetic background of the chromosome substitution laboratory mouse strain is based on a C57BL / 6 mouse, while wherein a number 1 chromosome is from a C3H / HeJ mouse. According to the construction for the number 1 chromosome substitution laboratory mouse strain C57BL / 6-Chr1C3H / HeJ, the chromosome substitution laboratory mouse strain can be used for research of character differences brought by different number 1 chromosome genomes under the same genome background, and can be a useful tool for research of the chromosome genome functions by adoption of a genetic method.

Methods for breeding mutagenized mice permit detection of genetic loci that in heterozygous mutated form can modify a known index phenotype involves crossing a mutagenized founder strain and a second strain of mice carrying an allele at a locus that confers the index phenotype. In the test generation, clusters of individuals are observed to deviate from the typical phenotype. The genetic material and molecules encoded thereby can be obtained using available methods. Improved and compact methods are also disclosed.

The invention relates to construction for Chromosome 1 to replace wild mus musculus strain C57BL / 6. Zaozhuang 2-Chr1. A novel Chromosome C57BL / 6 replacing experiment musculus strain is constructed, a C57BL / 6 musculus is mainly used as the genetic background of the musculus strain, and a Zaozhuang 2 musculus is mainly used in the Chromosome 1. The Chromosome replacing strain can be used for studying character difference brought by different Chromosome 1 genomes under the background of the same genome, and a useful tool for studying the functions of the genomes by using a genetics method is achieved.

The invention relates to construction for a number 1 chromosome substitution laboratory mouse strain C57BL / 6-Chr1AKR. According to the construction for the number 1 chromosome substitution laboratory mouse strain C57BL / 6-Chr1AKR, a new chromosome substitution laboratory mouse strain is constructed. A genetic background of the chromosome substitution laboratory mouse strain is based on a C57BL / 6 mouse, while wherein a number 1 chromosome is from an AKR mouse. According to the construction for the number 1 chromosome substitution laboratory mouse strain C57BL / 6-Chr1AKR, the chromosome substitution laboratory mouse strain can be used for research of character differences brought by different number 1 chromosome genomes under the same genome background, and can be a useful tool for research of the chromosome genome functions by adoption of a genetic method.

The present invention relates generally to the field of generating genetically modified C57 mice. More particularly, the present invention pertains to 1) blastocyst-derived mouse embryonic stem cell (ES) cell lines including, but not limited to, the IC1, IC2, IAC1, IAC2, IAC3, IAC4, IAC5, IAC6, IAC7 or IAC8 ES cell line, 2) to efficient methods of making genetically modified C57 mice by introducing the modified C57 ES cells into the mouse blastocysts of either the same mouse strain and / or color of albino C57 strain, or other C57 strain, to generate genetically modified novel, useful and hereto unknown models of C57 mice, and to methods for identifying the chimerism of chimeras which can be not known by coat color.

A method, called GETWISE, for targeting mouse genes is described. GETWISE is designed to increase the frequency of homologous recombination, facilitate screening, widen the applicability of engineered animals and circumvent intrinsic gene targeting problems. GETWISE utilizes the principle of modulating gene expression by targeting tetracycline-responsive elements into a specific locus. In GETWISE alleles, control of gene expression is transferred from the endogenous to a tetracycline-inducible promoter. Endogenous promoters now control expression of the reporter gene luciferase. Breeding of GETWISE carriers with tTA / rtTA carriers enables investigators to modulate gene expression in a ubiquitous or tissue-specific manner, depending on the presence of doxycycline. GETWISE enables the study of loss or gain of gene expression in any tissue of choice within a single mouse strain. GETWISE enables the analysis of the gene expression pattern with the luciferase assay.

The present invention relates generally to genetically modified non-human animals and immunodeficient non-human animals characterized by restored complement-dependent cytotoxicity, as well as methods and compositions for assessment of therapeutic antibodies in the genetically modified immunodeficient non-human animals. In specific aspects, the present invention relates to immunodeficient non-obese diabetic (NOD), A / J, A / He, AKR, DBA / 2, NZB / BIN, B10.D2 / oSn and other mouse strains genetically modified to restore complement-dependent cytotoxicity which is lacking in the unmodified immunodeficient mice. In further specific aspects, the present invention relates to NOD.Cg-Prkdcscid IL2rgtm1Wjl / SzJ (NSG), NOD.Cg-Rag1tm1Mom IL2rgtm1Wjl / SzJ (NRG) and NOD.Cg-Prkdcscid IL2rgtm1Sug / JicTAc (NOG) mice genetically modified to restore complement-dependent cytotoxicity which is lacking in unmodified NSG, NRG and NOG mice. Methods for assessment of therapeutic antibodies or putative therapeutic antibodies in the genetically modified immunodeficient mice characterized by an intact complement system are provided according to specific aspects of the present invention.

The present invention is related with the neurobiology and the transgenic animals, more specifically with obtaining a model to study the SCA2 genetic disease. The genome of one mice line has been modified through the introduction of a DNA segment that contains the necessary information for the synthesis of the human ataxin 2. In particular the invention is related with the transgenic mice F066 and the cellular lines able to express the human sca2 gene under the regulation of its self promoter. The F066 transgenic mice reproduce the features of the disease. The homozygous transgenic mouse line for the new gene was obtained through matting. This transgenic line is useful to study the mechanism that produce the disease and also will permit to prove new therapeutics that contributes to minimize the clinical symptoms in the patients.

The invention belongs to the field of genetic background identification and genetic contamination detection for inbred strain mouse strains, and relates to a SNP locus set used for genetic quality monitoring for a BALB / cJ inbred strain mouse and a primer combination and application of the SNP locus set. SNP loci comprise 11 specificity identification loci for a BALB / C strain, 73 feature loci for identifying some strain except the BALB / c strain and 12 universal loci, by synthesizing results of the 96 SNP loci, the consistence degree of inbred strain backgrounds of a tested mouse individual anda specific mouse can be precisely reflected, genetic characters and variation conditions of a genome of the tested mouse individual are comprehensively detected, and the genetic quality state of the tested mouse and the stability quality of an experiment are monitored. According to the SNP locus set and the primer combination and application thereof, reaction conditions are optimized for SNP primers in later detection, and sensitive, specific and efficient detection is ensured.

The invention provides a construction method of a concanavalin A induced mouse xerophthalmia model and application. The construction method of the mouse xerophthalmia model induced by the concanavalin A comprises the following steps that 10 microgram / milliliter of a PBS solution of the concanavalin A is prepared, the PBS solution is sub-packaged into 20 microliter / drop, one drop is sucked by using an insulin injection needle when the PBS solution is used, and the drop is injected into a lacrimal gland on one side of a mouse, the used mouse strain is a common BALB / c mouse, the price is lower, and the model forming rate is 70% or above. The method is a single injection method, the medicine utilization rate is low, the molding period is short, the xerophthalmia model induced by the method accords with part of clinical characteristics of the xerophthalmia, and the xerophthalmia mouse model can be applied to research and development of medicines for treating xerophthalmia.

The invention relates to the technical field of mouse strain identification, and particularly discloses application of an SNP (Single Nucleotide Polymorphism) marker in inbred line mouse strain identification and a primer sequence. Specifically, according to the application of the SNP marker in inbred line mouse strain identification, the SNP marker comprises 15 SNP loci; the inbred line mouse strain is selected from the group consisting of C57BL / 6J, C57BL / 6N, BALB / c, FVB, DBA / 2, CBAA / CaJ and C3H. The invention also discloses an application of the primer sequence for synthesizing the SNP site in inbred line mouse strain identification. According to the application, seven inbred line mouse strains of C57BL / 6J, C57BL / 6N, BALB / c, FVB, DBA / 2, CBAA / CaJ and C3H can be identified at the same time, and the accuracy of inbred line mouse strain identification results is improved.

Serotonin neurons modulate most homeostatic Central Nervous System (CNS) functions while influencing the expression of behavioral traits such as mood, aggression and anxiety. Serotonin neuron dysfunction has been implicated in depression, addiction, autism and sudden infant death syndrome. This disclosure describes a straightforward, highly reproducible method for genetically accessing serotonin neurons and a sub-population of intestinal enterocytes, in vivo, using BAC-based transgenes that can be constructed using simple subcloning procedures. Compositions described herein include these transgenes and methods for making and using them to create transgenic mouse strains and identify serotonin and intestinal enterocytes without immunostaining.

The invention discloses a method for constructing a human epidermolytic palmoplantar keratoderma mouse model. The method comprises the following steps: mutating A of the 434th nucleotide of mouse Krt9 genes into GGCT, constructing a targeting plasmid vector containing recombinant Krt9 genes shown as SEQ ID NO:1, and screening a mouse carrying the recombinant Krt9 genes, thereby obtaining the human epidermolytic palmoplantar keratoderma mouse model. The mouse is obtained by utilizing a gene targeting technology, an international first mouse strain of Krt9 gene knock-in mutation is screened, namely a Krt9-indel mouse, and the phenotype of the model is completely consistent with that of the human EPPK, so that a specific animal model of completely imitating the human EPPK from genes to the phenotype is obtained, and the model can be further widely applied to research of pathogenic causes and pathogenic mechanisms of human EPPK and screening of medicines for treating the EPPK and gene treatment tests.

The invention relates to a method for monitoring the genetic quality of inbred mice by using microsatellite technology. The present invention provides a series of microsatellite loci with good polymorphism and good polymorphism distributed on different chromosomes of mice and their specific primers, which are used for the identification and inheritance of commonly used inbred mouse strains Polymorphism monitoring. The method of the invention is simple and fast in operation, low in cost and easy to popularize and use.

This invention provides a human antibody, a hybridoma cell line for the production of the antibody, a reconstituted mouse strain for the production of the hybridoma, and methods of producing and using thereof.

The invention relates to an establishment of wild house mouse C57BL / 6. Songjiang 3-Chrl with chromosome 1 substituted. The invention constructs a new chromosome C57BL / 6 substituted laboratory mice strain, and the genetic background of the mice strain mainly employs C57BL / 6 mice, wherein, chromosome 1 mainly employs Songjiang 3 mice. The chromosome substitution strain is used for researching character differences brought by different chromosome genome 1 under the same genome background, and is a useful tool for researching chromosome genome functions using genetic method.

The invention relates to an establishment of wild house mouse C57BL / 6. Songjiang 3-Chrl with chromosome 1 substituted. The invention constructs a new chromosome C57BL / 6 substituted laboratory mice strain, and the genetic background of the mice strain mainly employs C57BL / 6 mice, wherein, chromosome 1 mainly employs Songjiang 3 mice. The chromosome substitution strain is used for researching character differences brought by different chromosome genome 1 under the same genome background, and is a useful tool for researching chromosome genome functions using genetic method.

The invention provides a method and system for automatically designing a mouse breeding scheme based on the Mendel's genetic law. The method comprises the steps of building a breeding scheme model which comprises a project type, the number of genes contained in each breeding route, an identification mode, a mouse strain background and a background mouse age; inputting item information and startingand target mouse information, and generating at least one feasible breeding scheme, wherein the breeding scheme at least comprises the breeding route and required cost; and selecting and determiningthe breeding scheme. According to the method, the mouse breeding scheme design process and logic based on the Mendel's genetic law are immobilized and standardized and can be sorted according to certain logic, result errors caused by errors of designers can be avoided, and the optimal scheme is derived. The automatic design greatly improves the design efficiency of the breeding scheme, and can save a large amount of labor cost.

The invention belongs to the technical field of gene engineering, and relates to construction and application of two Pparg gene site-directed mutagenesis mouse models. According to the invention, a CRISPR / Cas9 gene editing technology is used to carry out artificial mutation on the position of the Pparg gene for coding threonine at the 166th site, and thus two site-directed mutagenesis mouse strains are successfully manufactured. On the basis of the obtained Pparg gene site-directed mutagenesis mouse strains, various physiological and pathological states of the two strains are analyzed through various cell, biochemical and molecular biological research means, and the application value of the Pparg gene site-directed mutagenesis mouse strains in related research is determined. Meanwhile, the Pparg gene site-directed mutagenesis mouse can be applied to screening of related drugs, drug design, pharmacology / toxicology, pharmacodynamics, pharmacokinetics and exploration of therapeutic targets.

An object of the present invention is to provide a mouse which has the characteristics of early developing visceral fat type obesity and also has concurrent diabetes and hyperlipemia and in which the trait is genetically established and recessively inherited. An ICR-derived mouse strain, Daruma, spontaneously developing obesity, exhibiting autosomal recessive inheritance for the trait of spontaneously developing obesity, and becoming obese only in the homozygous type is provided.

The invention belongs to the technical field of biological medicine. The invention provides an application of orychophragmus violaceine D, namely an OV16 monomeric compound, in preparation of drugs for inhibiting small intestine crypt epithelial cell ferroptosis, the orychophragmus violaceine D OV16 can inhibit rat small intestine crypt epithelial cell ferroptosis induced by Erastin, and the orychophragmus violaceine D OV16 is an effective inhibitor for rat small intestine crypt epithelial cell ferroptosis induced by Erastin. Experiments prove that when the OV16 is used for treating 14.5 Gy abdominal irradiation cobalt-source mouse C57 strain, radiation injury of mouse intestinal crypt tissues can be protected, and a theoretical basis is provided for research on treatment and prevention of intestinal radiation injury by the orychophragine DOV16 in the future.

The invention relates to construction for a number 1 chromosome substitution laboratory mouse strain C57BL / 6-Chr1NZW. According to the construction for the number 1 chromosome substitution laboratory mouse strain C57BL / 6-Chr1NZW, a new chromosome substitution laboratory mouse strain is constructed. A genetic background of the chromosome substitution laboratory mouse strain is based on a C57BL / 6 mouse, while wherein a number 1 chromosome is from an NZW mouse. According to the construction for the number 1 chromosome substitution laboratory mouse strain C57BL / 6-Chr1NZW, the chromosome substitution laboratory mouse strain can be used for research of character differences brought by different number 1 chromosome genomes under the same genome background, and can be a useful tool for research of the chromosome genome functions by adoption of a genetic method.

The invention discloses a method for constructing a human epidermolytic palmoplantar keratoderma mouse model. The method comprises the following steps: mutating A of the 434th nucleotide of mouse Krt9 genes into GGCT, constructing a targeting plasmid vector containing recombinant Krt9 genes shown as SEQ ID NO:1, and screening a mouse carrying the recombinant Krt9 genes, thereby obtaining the human epidermolytic palmoplantar keratoderma mouse model. The mouse is obtained by utilizing a gene targeting technology, an international first mouse strain of Krt9 gene knock-in mutation is screened, namely a Krt9-indel mouse, and the phenotype of the model is completely consistent with that of the human EPPK, so that a specific animal model of completely imitating the human EPPK from genes to the phenotype is obtained, and the model can be further widely applied to research of pathogenic causes and pathogenic mechanisms of human EPPK and screening of medicines for treating the EPPK and gene treatment tests.