56 results about "Microgram" patented technology

The invention discloses mannheimia haemolytica outer membrane vesicles as well as a preparation method and application thereof, and belongs to the technical field of bioengineering. The OMV is separated from Mh-5 strain culture supernatant through tangential flow ultrafiltration combined with an ultracentrifugation technology, and animal experiments prove that a high-level IgG / IgM antibody can be induced by intramuscular injection of 50 micrograms per mouse, expression of inflammatory factors such as IL-6 and TNF-alpha of the spleen is remarkably improved, the protection rate after challenge reaches 85.7%, and pathological damage is remarkably lighter than that of an inactivated vaccine group. According to the scheme provided by the invention, the problems of insufficient serotype coverage and antibiotic resistance in the prior art are solved, and a new strategy is provided for prevention and control of mannheimia haemolytica.

The invention belongs to the technical field of biological materials and biological information, and provides a space phosphorylation modification omics detection method. According to the detection method disclosed by the invention, proteomics analysis can be carried out on trace sample phosphorylation, 4259 phosphorylation sites can be identified by 5 micrograms of peptide fragments, and the credibility of 3506 sites is greater than 0.75.

The invention relates to the technical field of immunochromatography analysis, and discloses a signal enhancer of a colloidal gold immunochromatography rapid detection card and a preparation method thereof, the signal enhancer comprises a silver ion solution, a thiourea-ascorbic acid synergistic reducing agent, a pH buffer system and a surfactant stabilizing system, the signal amplification is realized by catalyzing and depositing silver nanoparticles on the surface of colloidal gold, the operation is simple and convenient, one-time addition is realized, and the reaction is completed within 5-10 minutes; the sensitivity is improved by 5-10 times, and the specificity is higher than 98%; the stability is excellent, and the storage time is 12 months at 4 DEG C; various colloidal gold rapid detection cards are universally compatible; compared with the prior art, the colloidal gold immunochromatography rapid detection card has the advantages that the cost is controllable, the single detection is increased by 0.3-0.5 yuan, and the colloidal gold immunochromatography rapid detection card is particularly suitable for ultra-sensitive detection of small molecular substances in the field of food safety, for example, the detection limit of malachite green reaches 0.1 microgram per liter, the detection limit of fipronil reaches 0.5 microgram per liter, and the application range of the colloidal gold immunochromatography rapid detection card is obviously expanded.

The invention relates to a receptor reagent and application thereof. The receptor reagent comprises a buffer solution and receptor particles suspended in the buffer solution, the receptor particles can react with active oxygen to generate chemiluminescence, and the receptor reagent is characterized in that the ZETA potential of the receptor particles is not higher than 0 mV and not lower than-15 mV; the sugar content per milligram of receptor particles is greater than 40 micrograms. The receptor reagent provided by the invention can be produced on a large scale, is low in cost, qualified in quality and stable in performance, and can meet the sensitivity requirement and the linear range requirement; the kit containing the receptor reagent has the advantages of strong sample anti-interference capability and good test performance.

The invention relates to the technical field of breeding regulation and control of black goats, in particular to a hormone treatment method for inducing estrus synchronization of black goats, which comprises the following steps: placing a CIDR vaginal suppository containing 1.38-1.56 g of progesterone into the vagina of an ewe, and keeping for 12-14 days; 48 hours before the thrombus is removed, intramuscularly injecting a sustained release preparation of 330 IU follicle-stimulating hormone and 30% polyvinylpyrrolidone; when the thrombus is removed, intramuscularly injecting a mixed solution containing 330IU pregnant mare serum gonadotropin and 0.1 mg of cloprostenol; 24 hours after the thrombus is removed, 25 micrograms of luteinizing hormone is intravenously injected to release hormone A3; single-time artificial insemination is performed within 48-56 hours after injection, 0.5 ml of semen is left for each injection, and the number of effective sperms is not less than 1.5 * 10 < 8 >. By precisely regulating and controlling CIDR progesterone and multi-hormone stepped injection, the estrus synchronization rate of a group is larger than or equal to 95%, and the traditional synchronization bottleneck is broken through; insemination parameters are controlled and optimized management is carried out, each fetal live lamb is 2.5-3.0, and the breeding efficiency is improved; treatment is started 45-50 days after delivery, the lambing interval is 210 + / -10 days, and large-scale efficient breeding is assisted.

The invention discloses a diagnostic method based on mouse lyme disease serology indirect ELISA laboratory, and relates to the technical field of immunoserology detection.The diagnostic method comprises the steps that experimental conditions are optimized through a system, and the optimal reaction system is determined as follows: the antigen coating concentration is 0.2 microgram / microliter, the serum dilution ratio is 1: 200, and the enzyme-labeled secondary antibody concentration is 1: 2000. 0.8), and meanwhile, the antigen dosage is obviously reduced. Dynamic analysis of the antibody shows that the level of the antibody infecting mouse serum reaches a peak 12-25 days after inoculation, and the threshold value of the specific antibody ratio (ArB%) is 71.43%. Researches prove that the method has the characteristics of simplicity and convenience in operation, economy and high efficiency, and is suitable for primary laboratories and large-scale screening. The method provides reliable standardized technical support for lyme disease laboratory diagnosis and epidemiological monitoring.

The invention provides a method for rapidly detecting the content of theophylline in plasma, urine, medical sewage and functional beverages. According to the method, aiming at theophylline analytes in different samples, firstly, the samples are pretreated by adopting a liquid phase microextraction (LPME) technology, and then the pretreated theophylline is detected by adopting liquid phase-tandem mass spectrometry (LC-MS / MS). According to the method, an external standard method is adopted for quantification, the established method is good in linearity, the linear range is 0.01-10 mg / L, the correlation coefficient of theophylline is larger than 0.999, the detection limit is 0.2 microgram / L, the quantification limit is 0.7 microgram / L, the adding standard recovery rate is 86.7%-111.3%, and the relative standard deviation is smaller than 9%. The method is easy to operate and convenient to use, has the advantages of being good in accuracy, high in precision and sensitivity and the like, and provides a reliable method for rapid detection of theophylline in various actual scenes such as medical treatment, market supervision and environment monitoring.

A gravimetric solid-state dosing feeder system, configured for use in the movement of granular solids in an efficient manner while limiting jams and / or clogs in the feeder. The system employs bimorph benders in operation. The system provides rate and dosing accuracy to the limit of an individual pulse of material from the Pre-Feeder or depending on the feeder required flow rate, in the order of micrograms. The system is solid state in the sense that there are no bearings, gears, linkages, material shear plates and other moving parts. As such, it has the advantages of piezoelectric drives.

The invention discloses a CD147 nano antibody D2 as well as a preparation method and application thereof, and belongs to the technical field of molecular biology and immunology. The preparation method comprises the following steps: screening from an alpaca nano antibody phage library by utilizing a phage display technology, and sequencing to obtain 9 CD147 nano antibodies; 3 nano antibody genes are selected and cloned into pCold II, and prokaryotic expression recombinant plasmids are successfully constructed; the method comprises the following steps: carrying out inducible expression for 20 hours under the conditions that the final concentration of IPTG (isopropyl-beta-d-thiogalactoside) is 0.2 mmoL / L, the temperature is 16 DEG C and the speed is 100 rpm / min, carrying out nickel column purification, and then carrying out Coomassie brilliant blue dyeing and Western blot identification to determine and obtain the CD147 nano antibody which is named as C36, D2 and H4; the binding activity of the three nano antibodies and the CD147 protein is detected through indirect ELISA (enzyme-linked immuno sorbent assay). The result shows that the CD147 antigen and the three nano antibodies have binding activity and are dose-dependent. Under the same conditions, when the incubation concentration of the nano-antibody is as low as 1.25 micrograms / mL, the D2 binding effect is the best, the amino acid sequence of the nano-antibody is shown as SEQ ID NO.8, and the nucleotide sequence of the nano-antibody is shown as SEQ ID NO.10. The invention provides a more accurate method for detecting the CD147.

The invention discloses a mass spectrometric detection method for bisphenol A in milk through rotary evaporation pretreatment and multi-charge peak analysis. The mass spectrometric detection method comprises the following steps: step 1, carrying out rotary evaporation pretreatment on fresh milk; step 2, carrying out rotary evaporation treatment on the milk sample; 3, omics analysis of lipid, metabolites and protein is completed, and UHPLC-Q-Orbitrap HRMS strategy processing based on vDIA is achieved; and 4, analyzing the group database by adopting a multi-charge overlapping peak extraction algorithm and a graph neural network strategy to realize substance identification. The invention belongs to the technical field of mass spectrometry, and solves the problems that the detection error is large when microgram / L-level trace detection of bisphenol A in milk is carried out in the prior art, and false positive is easy to occur due to a wide mass window in the traditional extraction ion chromatography.

The invention relates to application of Antarctic red in repairing skin barrier injury and delaying skin aging, and belongs to the technical field of biology, the Antarctic red has no toxicity to cells when the concentration is not higher than 250 g / mL, when the concentration of the Antarctic red is 15.63-250 g / mL, the Antarctic red can promote repair of injured cells, the maximum improvement rate is 15.44% (plt; the anti-aging performance (plt, 0.05) of human fibroblasts and human immortalized keratinocytes can be remarkably improved, the effect is superior to or equivalent to that of positive control drug nicotinamide (plt, 0.001) when the concentration is 16 micrograms / mL, and the anti-aging composition can be used for repairing skin barrier injury and delaying skin aging.

The utility model discloses a colloidal gold test paper card for rapidly determining rice yeast acid in whole blood. The colloidal gold test paper card comprises a bottom plate as well as a glass cellulose membrane, a filtration binding area, a detection area and a water absorption pad which are sequentially arranged on the bottom plate from left to right, the filtration binding area adopts an antigen-antibody binding technology, red blood cells in whole blood can be retained on a filter membrane of a sample pad without moving, other substances in the whole blood can smoothly pass through, and the detection effect is good. According to the present invention, the kit can be combined with the rice yeast acid so as to eliminate the background interference, can be combined with the rice yeast acid, and can be detected through the detection region, such that the rice yeast acid in the whole blood can be accurately detected, the accuracy and the specificity of the rice yeast acid determination in the whole blood can be effectively improved, the application range and the efficiency of the trace whole blood sample in the detection can be expanded, and the redundant pretreatment operation is not required; the method is simple in operation and rapid in detection, and can obtain a result within 15 minutes only by taking 10 microliters of trace whole blood, and the lowest detection limit of 50 micrograms / L is reached.

A dry powder inhalation treatment for pulmonary arterial hypertension includes a dose of dry particles comprising greater than 25 micrograms of treprostinil enclosed in a capsule. The dry particles can include treprostinil, a wetting agent, a hydrophobicity modifying agent, a pH modifying agent and a buffer. A method of treating a patient having pulmonary arterial hypertension includes providing a patient a dry powder inhaler, providing the patient at least one capsule for use in the dry powder inhaler, the capsule including at least 25 micrograms of treprostinil.

The invention discloses a bionic polypeptide nano-composite skin permeation carrier and a preparation method thereof. According to the carrier, a biodegradable high polymer material is used as a nano core, hydrophobic active ingredients are loaded, and a nano composite system with a bifunctional bionic shell is formed through covalent immobilization of bionic cell-penetrating peptide and biological adhesion peptide. The preparation method comprises three steps of oil / water emulsification volatilization nucleation, peptide coupling modification and freeze drying. The obtained carrier has the characteristics of actively penetrating a skin barrier and long-acting residence, the encapsulation efficiency reaches 85% or above, the steady-state transdermal rate is increased to 3.7 micrograms / cm < 3 > / h, and the drug leakage rate is lower than 7% after the carrier is stored for 30 days. By simulating the natural mechanism of skin permeation and adhesion, efficient encapsulation, steady-state release and deep delivery of active ingredients are realized, and the preparation method is suitable for the fields of transdermal drug delivery, skin repair, beauty and the like, and has good application prospects and industrialization potential.

The invention belongs to the technical field of biological medicines, and particularly relates to a method for enhancing the activity of CIK cells and application of the CIK cells in tumor treatment. The method comprises the following steps: separating exosomes (the particle size is 50-150 nm, TSG101 and Alix are expressed, and CD9 and Calnexin are not expressed) from a CIK cell culture supernatant through an ultracentrifugation method, and transfusing back according to the proportion that every 2 * 10 < 7 > CIK cells are combined with 100-500 [mu] g of exosomes. The exosome can significantly improve the IFN-gamma expression level of CIK cells and promote PBMC proliferation, and in a liver cancer mouse model, it is proved that the tumor inhibition rate (70.7%) of a combined group (2 * 107 CIK cells + 400 micrograms of exosome) is significantly superior to that of a single treatment group. Waste exosome resources are utilized, the anti-tumor activity of the CIK cells is safely enhanced, and the CIK cells are suitable for treatment of solid tumors such as liver cancer, lung cancer and breast cancer and hematologic tumors.

Provided are novel serum-free culture media which comprise basic fibroblast growth factor (bFGF), transforming growth factor beta-3 and ascorbic acid at a concentration of at least about 50 microgram / ml; ascorbic acid at a concentration range of about 400-600 microgram / ml, bFGF at a concentration range of about 50-200 ng / ml, xeno-free serum replacement and a lipid mixture; the IL6RIL6 chimera at a concentration range of about 50-200 picogram per milliliter (pg / ml); or leukemia inhibitory factor (LIF) at a concentration of at least 2000 units / ml; cell cultures comprising same with pluripotent stem cells such as human embryonic stem cells and induced pluripotent stem (iPS) cells, and methods of using same for expanding pluripotent stem cells in an undifferentiated state using two-dimensional or three-dimensional culture systems; and methods of expanding iPS cells in a suspension culture devoid of substrate adherence and cell encapsulation.

The present application relates to the technical field of immunochromatographic analysis, and discloses a signal enhancer for a colloidal gold immunochromatographic rapid detection card and a preparation method thereof, the signal enhancer comprises a silver ion solution, a thiourea-ascorbic acid synergistic reducing agent, a pH buffer system and a surfactant stabilizing system, signal amplification is realized by catalytic deposition of silver nanoparticles on the surface of colloidal gold, the present application is simple to operate, is added at one time, and reaction is completed in 5-10 minutes; sensitivity is improved by 5-10 times, specificity is higher than 98%; stability is excellent, 12 months of storage at 4 DEG C; it is compatible with various colloidal gold rapid detection cards; cost is controllable, single detection is increased by 0.3-0.5 yuan, and the present application is especially suitable for super-sensitive detection of small molecule substances in the field of food safety, such as detection limit of malachite green of 0.1 microgram per liter, detection limit of fipronil of 0.5 microgram per liter, and the application range of the colloidal gold immunochromatographic rapid detection card is significantly expanded.

The invention belongs to the technical field of microbial engineering, and particularly provides preparation and application of selenium-enriched bacillus natto. Bacillus natto JLNA2301 is subjected to primary fermentation through a liquid culture medium, and a seed culture solution is obtained; inoculating the seed culture solution into a liquid culture medium containing inorganic selenium for secondary fermentation to obtain a selenium-rich strain fermentation solution; the inorganic selenium conversion rate of the strain is 97%, and the selenium content of the strain reaches 165 micrograms / g; the selenium-enriched bacillus natto can improve the reduction of the body mass of the aged animal, enhance the muscle strength and the active degree of the aged animal and improve the learning and memory ability of the aged animal; meanwhile, the selenium-enriched bacillus natto can regulate oxidative stress levels of serum, liver, kidney, skeletal muscle and the like of the aged animals, reduce levels of inflammatory factors such as TNF-alpha, IL-1beta and IL-6 of the serum, and antagonize liver injury caused by aging by regulating and controlling a PI3K / Akt signal channel.

The invention discloses garcinia cambogia fat-reducing and shaping health-care cream. Targeted delivery of active components (HCA, escin and the like) is realized through a nano-liposome drug-loading technology. In-vitro experiments prove that the fat decomposition rate (67.5%, Plt, 0.01) of the product can be remarkably increased, the transdermal absorption amount reaches 38.9 micrograms / cm < 3 >, and the stability of the paste is excellent (the retention rate of effective components is greater than 90% after 3-month acceleration at 40 DEG C). The composition is suitable for external application to improve local fat accumulation.

To provide a dry powder inhalation treatment for pulmonary arterial hypertension, a method of treating a patient suffering from pulmonary arterial hypertension, a dry powder inhalation composition for treatment, and a method of producing particles for delivering a dry powder to the lungs of a patient with pulmonary arterial hypertension.SOLUTION: The dry powder inhalation treatment for pulmonary arterial hypertension includes a dose of dry particles comprising more than 25 micrograms of treprostinil enclosed in a capsule. The dry particles may include treprostinil, a wetting agent, a hydrophobicity modifying agent, a pH modifying agent, and a buffer. A method of treating a patient suffering from pulmonary arterial hypertension comprises the steps of providing the patient with a dry powder inhaler, and providing the patient with at least one capsule for use in the dry powder inhaler, the capsule containing at least 25 micrograms of treprostinil.SELECTED DRAWING: Figure 1

The invention relates to the technical field of pesticide residue detection, in particular to a preparation method of a cyhalothrin colloidal gold quantitative test strip, and solves the technical problems of non-uniform antibody labeling, coating layer cracking and low detection sensitivity in the traditional process. Taking 1 ml of 40-nanometer colloidal gold particles, adding potassium carbonate, mixing and reacting for 5-15 minutes, then adding 5-7 micrograms of cyhalothrin monoclonal antibody, reacting, and finally adding confining liquid to prepare a colloidal gold labeled antibody solution; spraying the obtained solution on a glass fiber pad according to the spraying amount of 9.0-10.8 microliters per centimeter to prepare a gold-labeled pad; a specific coating buffer solution is used for preparing a quality control line solution and a detection line solution of 0.7-0.9 mg / ml cyhalothrin-BSA antigen, a nitrocellulose membrane is coated with the quality control line solution and the detection line solution, and after confining liquid treatment, centrifugation and resuspension, the nitrocellulose membrane is dried for 70-74 hours in inert gas with the temperature of 45 DEG C and the temperature of 2 DEG C; the test strip prepared by the method is used for high-precision quantitative detection of cyhalothrin residues in agricultural products.

The invention provides a detection method of Ruyi golden yellow powder, and belongs to the technical field of quality control of traditional Chinese medicine preparations. According to the method, apigenin is used as a reference substance, the content of total flavonoids in Ruyi golden yellow powder in terms of apigenin is calculated by preparing a reference substance solution, establishing a standard curve and determining the absorbance of a sample to be detected by adopting ultraviolet-visible spectrophotometry, if the content of apigenin in Ruyi golden yellow powder to be detected is not lower than 20 micrograms / g, the detection is qualified, otherwise, the detection is qualified, and the content of total flavonoids in Ruyi golden yellow powder to be detected is not lower than 20 micrograms / g. Unqualified products are obtained. The method can be used for objectively, comprehensively and accurately evaluating the quality of the Ruyi golden yellow powder, and has important significance on guaranteeing the quality of the product while guaranteeing the curative effect of the product.

The invention discloses a nano antibody T92-6 of an anti-tetanus toxin Hc structural domain and application of the nano antibody T92-6, and belongs to the field of immunotherapy biotechnology pharmacy. The nano antibody or the antigen binding fragment thereof comprises three complementarity determining regions CDR1, CDR2 and CDR3, the amino acid sequence of the CDR1 is SEQ ID NO: 6, the amino acid sequence of the CDR2 is SEQ ID NO: 7, the amino acid sequence of the CDR3 is SEQ ID NO: 8, and the sequences of the complementarity determining regions are defined according to an IMGT numbering system. The antibody is only combined with THc protein, and poisoning caused by a lethal dose of tetanus toxin (10 * LD50) can be effectively blocked by 5 micrograms of the antibody; after being humanized, the antibody still has better affinity and neutralizing activity, and the antibody with the same dose can still effectively block poisoning caused by tetanus toxin (10 * LD50) with a lethal dose.

The invention is applicable to the technical field of biology, and provides a streptococcus suis type 2 multi-epitope inhalation type subunit vaccine and application thereof. According to the invention, 4 CTL epitopes, 7 HTL epitopes and 6 B cell epitopes are screened out aiming at conserved epitopes of SS2 virulence factors SSU05-1022 and SpaA, the epitopes are fused by AAY / GPGPG / EAAAK linkers, L7 / L12 ribosomal protein is introduced as an adjuvant, and the inhalation type subunit vaccine 1022-SpaA V3 is constructed; the vaccine can improve the levels of sIgA and serum IgG of respiratory mucosa and activate related immune cells by virtue of 60 micrograms of intranasal primary immunization-enhanced immunization, so that a mucosa and system dual immune mechanism is formed; the survival rate of mice after SS2 multi-strain challenge reaches up to 100%, and the bacterial load can be reduced; and the vaccine is safe and non-toxic, is inhaled, saves cost and is suitable for industrial large-scale production.

The invention provides a method for constructing a myositis-related interstitial lung disease mouse model, which comprises the following steps: injecting 10 micrograms of ODN2395 into the abdominal cavity of a female BALB / c mouse, injecting 0.25 mL of a mixed emulsion of SD (Sprague Dawley) rat skeletal muscle homogenate and a complete Freund's adjuvant into each of the two sides of the back of the female BALB / c mouse at the same time point, and feeding for a certain time to obtain a film-formed mouse. The method has the advantages that raw materials are easy to obtain, and the modeling method is simple; 2, the modeling time is relatively short; and 3, the model forming rate is high, and the method is more in line with the pathogenesis and pathological phenotype of IIM-ILD.

The invention relates to a fluorescence detection reagent for on-site rapid classification and identification of synthetic cannabinoid. According to the detection reagent, based on a steric hindrance engineering thermodynamic gating strategy, a fluorescent probe molecule tert-butyl (4-(2-(4-hydroxybutyl)-1, 3-dioxo-2, 3-dihydro-1H-benzo [de] isoquinoline-6-yl) phenyl) carbamate containing a specific steric hindrance group (Boc group) is designed and synthesized. And the probe forms a fluorescence quenching aggregate in a thermodynamic metastable state in a system. The aggregate constructs a specific energy threshold value, and only when the aggregate encounters synthetic cannabinoid of a high affinity category (simultaneously having a keto / ester head and a hydrophobic tail chain), dissociation of the aggregate is synergistically triggered through multiple non-covalent interaction, and a remarkable fluorescent lightening signal is generated. The response time is less than 1 s, the detection limit can reach the microgram level, and the method has the characteristics of simplicity and convenience in operation, strong anti-interference performance (no false positive), convenience in visual observation and the like. The problem that a traditional single-molecule probe is difficult to classify and identify synthetic cannabinoid in a complex matrix is solved, and powerful technical support is provided for on-site investigation of drugs.

A dry powder inhalation treatment for pulmonary arterial hypertension includes a dose of dry particles comprising greater than 25 micrograms of treprostinil enclosed in a capsule. The dry particles can include treprostinil, a wetting agent, a hydrophobicity modifying agent, a pH modifying agent and a buffer. A method of treating a patient having pulmonary arterial hypertension includes providing a patient a dry powder inhaler, providing the patient at least one capsule for use in the dry powder inhaler, the capsule including at least 25 micrograms of treprostinil.

The invention belongs to the technical field of biological materials and tissue engineering, and discloses a culture medium capable of directionally up-regulating expression of endogenous N-cadherin of hMSCs (human mesenchymal stem cells) as well as a preparation method and application of the culture medium, a fusion protein of a nerve cell cadherin extracellular domain 1-2 and an Fc segment is fixed on the surface of the medium, and the amino acid sequence of the fusion protein is shown as SEQ ID NO: 1. The problems that natural N-cadherin is short in half-life period and unstable in surface adsorption are solved through Fc segment modification. Experimental data show that the fixing efficiency of the N-cad1-2-Fc fusion protein on the surface of polystyrene is remarkably improved, the ELISA detection OD value reaches 1.2 under the concentration of 10 micrograms / mL, and the protein is uniformly distributed and cannot be lost due to physical flushing or time lapse.

The utility model provides a high-precision dynamometer calibration device, and relates to the technical field of dynamometer calibration. A high-precision dynamometer calibration device comprises a reference assembly and a force application assembly. The reference assembly comprises a base and a balance rod which is arranged on the base in a centrally rotating mode. The force application assembly comprises telescopic parts symmetrically arranged on the two sides of the balance rod, the fixed ends of the telescopic parts are fixedly connected to the base, the telescopic ends of the telescopic parts are detachably connected with a dynamometer, the dynamometer is detachably connected with the balance rod, a first distance sensor is arranged between the dynamometer and the base, and a second distance sensor is arranged between the base and the base. The first distance sensor is used for monitoring the stroke change of the two dynamometers and the telescopic action of the two telescopic pieces, so that the accuracy and reliability of a measurement result can be ensured when force values of small to microgram level and large to kilogram level are accurately measured and calibrated.