70 results about "Coacervate" patented technology

The present invention is drawn to an adhesive or binder composition comprising, —a cationic polyelectrolyte, —an anionic polyelectrolyte, —a mineral salt selected from the group consisting of alkaline metal or alkaline earth metal halogenides, —a particulate mineral filler, —a water-soluble polyphenol comprising at least one polyhydroxylated aromatic ring structure, and —water It is also drawn to the use of such a composition as an adhesive, binder, coating or sealant in the construction industry.

The invention discloses a preparation method of a micron-sized cell-matrix co-polymer biological material. The preparation method comprises the following steps: firstly, inducing SHED cells in an ultra-low adsorption plate by using an alpha-MEM culture medium containing serum, GlutaMAXI, vitamin C and the like to form micron-sized spheres (SHED spheres); secondly, taking human dentin, and carrying out mechanical crushing, EDTA (Ethylene Diamine Tetraacetic Acid) demineralization, grinding and sterilization to prepare 1-100 microns of dentin matrix particles (DDMPs); and finally, by taking the GelMA hydrogel as a carrier, entrapping SHED spphere and DDMPs, and carrying out 405nm photo-crosslinking for 30 seconds to form the hydrogel. The co-polymer can significantly promote angiogenesis and osteogenesis coupling regeneration in a bone defect model, and is suitable for oral cavity and cranio-maxillofacial bone repair.

The present invention proposes a method and use for stabilizing a simple agglomerate for pH and salt and with adjustable size. In phosphoric acid buffer solutions with different salt concentrations and pH values, the polyacrylic acid hydroxypropyl group condensation body has specific stability; hydrophobic thermal response blocks are introduced into a hydroxypropyl acrylate monomer through reversible addition-fragmentation chain transfer polymerization to regulate and control the size of a condensation body, and a phase separation behavior is shown at different temperatures; a hydrophobic block without thermal response is introduced to regulate and control the size of the aggregate, and no thermal response behavior is shown. Continuous regulation and control of the size of the aggregate from the micron level to the nano level are achieved, and the requirements of different application scenes are met. The hydroxypropyl polyacrylate condensation body can be used for packaging various dyes and drugs; high-efficiency enzyme protection is achieved, formed aggregates can protect glucose oxidase, and the retention rate of enzyme activity reaches 85% or above after treatment is conducted for 3 hours at 60 DEG C; as a micro-reactor for the reaction of generating p-nitrobenzaldehyde phenylhydrazone from p-nitrobenzaldehyde and phenylhydrazine, the reaction rate is greatly improved.

A non-sulfate shampoo is disclosed. The shampoo incorporates at least one non-sulfate surfactant selected from a taurate salt and a sarcosinate salt, and in addition incorporates a complexation compound. The shampoo produces a coacervate upon dilution with water and in use to shampoo hair. The shampoo provides mild non-irritating treatment of the hair and does not irritate skin or eyes.

A stimulation fluid to prevent coacervate formation in a subterranean formation. The stimulation fluid generally comprises nanobubbles, a cationic component, and an anionic component. The cationic component may be a biocide, scale control additive, clay control additive, water control or friction reducing polymer, surfactant, or a combination of cationic components. The anionic component may be a surfactant, scale inhibitor, chelant, water control or friction reducing polymer, or a combination of anionic components.

The invention belongs to the technical field of algal polysaccharide extraction, and discloses a modification extraction method of algal asparagus polysaccharide and a method for preparing a lutein coating by using the algal asparagus polysaccharide. The modified extraction method comprises the following steps: extracting gracilaria lemaneiformis powder with a weak base solution, enabling lactide and polysaccharide to be subjected to grafting reaction through a redox initiation system under the assistance of ultrasonic waves, and then separating out a first polysaccharide component with high emulsibility and a second polysaccharide component with high gel property through graded alcohol precipitation. The two obtained functional components are used for coating xanthophyll, specifically, the first polysaccharide component emulsifies a xanthophyll oil phase to form a pre-emulsion, the second polysaccharide component is compounded with gelatin, a composite coacervate is formed on an oil drop interface by adjusting the pH value, and finally drying and curing are conducted. According to the method, two modified polysaccharides with definite functions can be synchronously obtained, the prepared xanthophyll coating is high in encapsulation efficiency, good in storage and processing stability and excellent in water dispersibility, and the problems that xanthophyll is unstable and difficult to apply are effectively solved.

The invention discloses a special membrane material for targeted trapping of macrobrachium larvae in water and a preparation method of the special membrane material, and belongs to the field of water environment biological pollution prevention and control. The membrane material is of a multi-layer composite structure and comprises a nanofiber membrane base material layer, an interface adhesion layer and a functional modification layer. The interface adhesion layer is bionic coacervate gel formed by curing hydrophobic polyelectrolyte and phytic acid; the functional modification layer is covalently grafted with a bionic adhesion protein active peptide fragment and a macrobrachium larva chemical pheromone. The material actively attracts larvae to approach through chemical pheromones, high-strength adhesion capture is achieved through bionic peptide fragments rich in aromatic and cationic amino acids, and trapping is integrated. The preparation method comprises the steps of electrostatic spinning of a base material, surface activation, gel coating, functional modification and the like. The material disclosed by the invention has high selectivity and high capture rate on the larvae of the Limnoperna fortunei, is environment-friendly, long-acting and stable, and is suitable for green prevention and treatment of fouling of the larvae of the Limnoperna fortunei in facilities such as water pipelines and pump stations.

A method of breaking down at least one component of a substrate can include providing a catabolic nanocompartment that includes a liquid coacervate phase, at least one catalyst within the coacervate phase, and an aqueous phase surrounding the coacervate phase. The method can include contacting the catabolic nanocompartment with the substrate including the at least one component such that the catalyst degrades the at least one component and forms degradation products of the at least one component.

The invention discloses a macrophage immune activation method based on aggregate mediated magnesium ion delivery. The method comprises the following steps: in a buffer environment containing magnesium ions, preparing a biomolecular aggregate formed by compounding polyarginine and polyadenylic acid through liquid-liquid phase separation, and enriching the magnesium ions by the aggregate through multivalent chelation; diluting the aggregate loaded with high-concentration magnesium ions in a cell culture medium to obtain a biomolecular aggregate suspension; and sequentially adding the target cell suspension and the biomolecular aggregate suspension into a cell culture dish, fully mixing, and then transferring the cell culture dish into a carbon dioxide cell incubator for standing culture. According to the method, the biomolecular aggregate loaded with the high-concentration magnesium ions is co-cultured with the cells, and the high-concentration magnesium ions are delivered into the cells in a short time, so that the immune level of the cells is improved, and the activity and safety of the cells are ensured.

The present application relates to a kind of mussel-based composite coacervate based on mussel adhesive protein and its preparation method and purposes, the mussel-based composite coacervate includes mussel adhesive protein and anionic polyelectrolyte material;The mass ratio of mussel adhesive protein and anionic polyelectrolyte material is (0.05~4):1;The oxidation rate of dopa residue in the mussel adhesive protein in the composite coacervate is 0.1%~70.0%.The present application realizes the accurate control of the preparation of the composite coacervate formed by regulating the concentration, mass ratio and system pH of mussel adhesive protein and anionic polyelectrolyte material, and then improves its performance and effect as tissue adhesive, wound repair dressing, acute hemostatic material, local drug delivery carrier and drug release system field or bone tissue repair, cartilage repair and tissue filling and support field.

The invention relates to the technical field of biology, and discloses a polyelectrolyte aggregate high-strength hydrogel based on thermal induction crosslinking and a preparation method thereof, and the preparation method comprises the following steps: (1) preparing a solution; (2) forming a polyelectrolyte aggregate; (3) forming a precursor; (4) thermally induced crosslinking; and (5) post-treatment. The preparation method of the polyelectrolyte aggregate high-strength hydrogel based on thermally induced crosslinking provided by the invention adopts a two-step process: firstly forming a polyelectrolyte aggregate, and then carrying out heat treatment on the aggregate to induce the aggregate to strengthen; heat-induced endogenous crosslinking is adopted, namely endogenous chemical crosslinking such as Maillard reaction between chitosan and sodium hyaluronate is initiated by heating, so that the mechanical property order of magnitude is improved.

The invention relates to a liquid-liquid phase separation mediated condensate carrier and application thereof. Specifically, the invention provides a delivery carrier, which contains (a) a polypeptide and (b) a polynucleotide, wherein the polypeptide has a sequence as shown in (LRLLRWC) n, or the polypeptide has a sequence as shown in (LRLLR) nWC, and n is an integer greater than or equal to 1.

1. Name of the designed product: condensation bead (windmill type). 2. Use of the designed product: for daily washing. 3. Design points of the designed product: in shape. 4. Picture or photo that best shows the design points: perspective view.

The invention provides a liquid-liquid condensed phase microcapsule as well as a preparation method and application thereof, and belongs to the technical field of pesticides. The liquid-liquid condensed phase micro-capsule is a liquid-liquid condensed phase liquid drop wrapped by an interfacial film; the interfacial film comprises sodium polylipoate; the liquid-liquid condensed phase liquid drops comprise sodium lipoate and a cationic surface active agent, and the concentration of the sodium lipoate is 5-100 mM; and the concentration of the cationic surface active agent is 5-50 mM. The interfacial film of the liquid-liquid condensed phase microcapsule can effectively obstruct interaction between adjacent liquid drops, avoid spontaneous fusion of the liquid drops and guarantee the structural stability of the microcapsule in the long-term storage and transportation process. The liquid-liquid condensed phase microcapsule can entrap hydrophobic drug molecules to form a water-based carrier pesticide, and the wetting, spreading and deposition of the pesticide on hydrophobic leaf surfaces can be improved.

1. Name of the design product: condensation beads (four cavity-surrounding ball) 2. Use of the design product: the design product is used for washing clothes, and can also be used for washing dishes in a dishwasher. 3. Design points of the design product: in shape. 4. Picture or photo that best indicates the design points: perspective view.

This disclosure generally relates to microparticles containing algal proteins and their use in various applications. In some embodiments, the microparticles are precipitates. In some other embodiments, the microparticles are coacervates. In some embodiments, the microparticles are core-shell microcapsules having a hydrophobic core material encapsulated by a shell containing algal proteins. In some embodiments, the hydrophobic core material includes flavoring oils, fragrance oils, or combinations thereof. In certain embodiments, this disclosure provides the use of such microparticles to improve the texture, mouthfeel, perceived fattiness, or perceived creaminess of food products. In some embodiments, the food products are vegan dairy products, vegan meat products, or vegan seafood products. In certain embodiments, this disclosure provides the use of microparticles to provide fragrance to scented products such as personal care products, laundry products, or cosmetics.

The application discloses a design and application of a temperature-sensitive solid-liquid phase change peptide-based condensate probe, and belongs to the field of biomedical technologies. The peptide-based probe can form a condensate droplet through solid-liquid phase change near body temperature, and forms a gel at room temperature, can penetrate a cell membrane and produce fluorescence when combined with RNA.

The application belongs to the technical field of emulsion gels and salt-reduced mayonnaise, and particularly relates to application of a composite coacervate salt in preparation of salt-reduced mayonnaise and an emulsion gel constructed therefrom. The application constructs an emulsion gel by using a specific type of protein-polysaccharide-based composite coacervate salt. The gel not only has excellent oil embedding rate and physical stability, but also endows the system with significant salty taste perception characteristics. Based on the good stability and uneven distribution of salt, the emulsion gel can achieve the effect of significantly reducing salt without reducing saltiness, thereby meeting the salt reduction demand without sacrificing the sensory quality, and thus showing important application potential in the development of mayonnaise substitutes. Further, the composite coacervate salt is directly applied as a salt substitute in mayonnaise products, and a new type of mayonnaise can be constructed. Compared with traditional commercial mayonnaise, the new type of mayonnaise has more significant salty taste perception effect under the condition of a significant reduction in salt content, and thus shows broad application prospects.

The application discloses an oil-in-water probiotic emulsion based on a heterologous protein complex coagulation system and a preparation method and application thereof. The preparation method is as follows: first, a mixed solution of lactoferrin and bone bridge protein is prepared, and then the solution is stirred and dissolved at room temperature to hydrate; the pH is adjusted to 4.6-5.4 to obtain an aqueous phase; probiotic powder is dispersed in medium-chain triglyceride to obtain an oil phase; and the aqueous phase and the oil phase are mixed and homogenized to obtain the oil-in-water probiotic emulsion. The application has the advantages of simple preparation process, low cost, no need to add an emulsifier, no need to heat and add a crosslinking agent for strengthening the stability of the aqueous phase, high embedding rate of the oil-in-water emulsion probiotic, double protection, improved gastrointestinal tolerance and environmental resistance of the probiotic in the emulsion, and prolonged shelf life of the product, which expands the application of the probiotic and the protein in the food field.

The application provides a transmembrane drug delivery system and a preparation method thereof, and belongs to the field of nanometer drug carriers. The drug delivery system is a coacervate droplet formed by nanometer droplets caused by a bio-orthogonal reaction of a cell penetrating peptide and a disordered structure protein in situ at a cell membrane, and the coacervate droplet carries drugs into cells for drug delivery. In this way, the prepared transmembrane drug delivery system can improve the efficiency of delivering the load across the cell membrane.

Described herein are methods of characterizing incorporation of an agent into condensates, methods of reducing transcription of an oncogene associated with a condensate, and methods of using a peptide to inhibit binding of a nuclear receptor and a co-factor in a condensate.

The embodiment of the application provides a drug carrier based on a disordered structure protein and a cell penetrating peptide and a preparation method thereof, and belongs to the field of nanometer drug carriers. The drug carrier is a nanometer coacervate droplet existing in water phase freely, the nanometer coacervate has a core-shell structure, wherein the shell layer is a liquid phase layer of a cell penetrating peptide and a hydrophilic fragment of a disordered structure protein, is used for contacting with a cell membrane and loading a high molecular weight hydrophilic macromolecule; the core layer is a solid phase or a solid-like phase layer of the disordered structure protein with a hydrophobic fragment, is used for loading a hydrophobic small molecule; the particle size of the nanometer coacervate is 50-200 muM, and the Zeta potential is +5-+10 mV. In this way, a drug delivery system across a cell membrane can be prepared, and the efficiency of delivering a load across the cell membrane can be improved.

The application belongs to the technical field of protein purification, and discloses a protein purification method based on condensate, which comprises the following steps: firstly, constructing a target expression vector; secondly, expressing a fusion protein with an LplA label in E. coli; thirdly, ultrasonically crushing the E. coli, centrifuging the crushing liquid, and taking supernatant; fourthly, performing first temperature change, heating the supernatant, centrifuging and collecting the fusion protein after the fusion protein forms condensate, discarding the supernatant, and taking the precipitate; and fifthly, performing second temperature change, redissolving the precipitate obtained in the fourth step in a buffer at low temperature, removing insoluble proteins by centrifugation at low temperature, and taking the supernatant as the fusion protein with the LplA label after purification. The application connects the temperature-responsive purification label LplA which can form condensate to the target protein, and only needs to perform two temperature change treatments to quickly obtain the target protein with high purity, without the need of complex chromatography equipment and expensive fillers, and the application is suitable for large-scale production and purification of functional proteins.

A subject of the invention is a remineralizing dental material, having at least one cation and at least one anion, which are configured as partner ions for forming a remineralization substance. In accordance with the invention at least one of the partner ions is multivalent and is included as counterion in a coacervate. A further subject of the invention is a kit for producing such a dental material, and also the use thereof.

This invention belongs to the field of emulsion polymerization technology, specifically relating to a method for preparing coagulation-dispersion controllable emulsions based on acrylic macromolecular emulsifiers. The method utilizes acrylic macromolecular emulsifiers in a free radical emulsion polymerization process, covalently binding hydrophilic acrylic acid segments to the surface of latex particles. The coagulation and dispersion states of the emulsion prepared by this method are easily controlled by the pH value of the emulsion system. The control process is reversible and rapid. After a coagulation-dispersion cycle, the particle size and particle size distribution of the latex particles are essentially consistent with the initial emulsion. This method can significantly shorten the preparation cycle of high-solids-content emulsion polymerization, providing an effective approach for expanding the preparation and application of water-dispersible polymers.