The invention provides an
alginate lyase mutant Amut8C as well as a coding
gene and application thereof, belongs to the technical field of
gene engineering and
enzyme engineering, and can solve the problem that existing
alginate lyase cannot meet the requirements of severe
reaction conditions such as high temperature and acidity in industrial application. The
amino acid sequence of the
alginate lyase mutant Amut8C disclosed by the invention is as shown in SEQ ID NO. 1. According to the space structure of the alginate
lyase Algt, disulfide bonds in molecules are analyzed, the alginate
lyase is mutated, and the obtained alginate
lyase mutant Amut8C has
heat resistance, acid and alkali resistance and
digestive enzyme tolerance, and compared with the alginate lyase Algt, the alginate lyase mutant Amut8C has the advantages that the content of the alginate lyase mutant Amut8C is increased, and the content of the alginate lyase mutant Amut8C is increased. The alginate
oligosaccharide with the
polymerization degree of 1-4 prepared from the alginate lyase mutant Amut8C under the same enzymolysis condition is higher in yield. The method can be applied to the fields of feeds, foods, detergents or biofuels.