28 results about "Sequence homology" patented technology

A complex bait data set construction method based on structure and sequence collaborative redundancy elimination belongs to the field of bioinformatics, and comprises the following steps: screening an initial protein complex structure set, removing entries containing nucleic acids, small molecules or non-protein chains, and selecting binary complexes meeting integrity and resolution requirements; secondly, structure clustering and sequence clustering are carried out based on three-dimensional structure similarity and sequence homology, combined comparison is carried out on the two results, and redundant compound entries which are highly similar in structure and sequence are removed; then, taking each cluster representative compound as a target, generating a plurality of groups of bait structures by using a molecular docking or prediction modeling method, and calculating a quality index; and finally, performing stratified sampling and proportion balance based on the score interval of the quality index, and constructing a high-quality protein complex bait data set with structure and sequence collaborative redundancy elimination and balanced quality distribution. The data set generated by the method has the advantages of low redundancy, high diversity and quality distribution controllability.

The present invention relates to the field of allergens and more specifically to a novel isolated allergen from the species Cupressaceae shown to be a primary sensitizer for severe peach allergy mediated by the protein Pru p7. The allergen is homologous with Pru P7 in peach, a protein belonging to the Gibberelin Regulating Proteins (GRPs). The novel allergen also 5 shares high sequence homology among other species from the Cupressaceae family and there is therefore also provided additional novel allergens in two other species of the Cupressaceae family. The novel allergens find use in the in vitro diagnosis of, treatment and / or prevention of Type 1 Cupressaceace pollen allergy and Cupressaceace pollen-associated food allergies.

To provide a new agent for controlling soil-borne plant diseases, containing a microorganism as an active ingredient.SOLUTION: An agent for controlling a soil-borne plant disease, comprising, as an active ingredient, a Pseudomonad bacterium having a 16SrRNA genome comprising a nucleotide sequence having 96% or more sequence identity to a nucleotide sequence represented by SEQ ID NO: 1, a Pseudomonad bacterium having a 16SrRNA genome comprising a nucleotide sequence having 98% or more sequence identity to a nucleotide sequence represented by SEQ ID NO: 2, or a combination thereof.SELECTED DRAWING: None

The invention provides a fusion transcript recognition method, device and system based on transcriptome multiple comparison data and a medium, and the core of the method is to construct a set of full-chain analysis framework oriented to multiple mapping reads to realize fusion gene detection. Comprising a pairing read segment preliminary screening module based on a loose mapping strategy, a candidate fusion construction module based on an exon graph structure, a remapping module based on an enhanced reference transcriptome, a transcript abundance estimation module based on a probability generation model, and a fusion scoring module based on fusion site specificity support degree. And a false positive inhibition module based on coverage consistency and biological filtration. According to the method, the dependence of a traditional fusion gene detection method on a unique mapping read is broken through, multiple comparative analysis on the fuzzy mapping read abandoned due to sequence homology is proposed for the first time, and high sensitivity and low false positive rate of the fuzzy mapping read are verified in simulated data and real tumor samples; the method has remarkable technical advancement, clinical applicability and expandability.

The invention discloses an intestinal symbiotic bacterium for enhancing baculovirus resistance of spodoptera frugiperda and application thereof, and relates to the technical field of microorganisms, the intestinal symbiotic bacterium is derived from the midgut of the spodoptera frugiperda, is separated and purified and is subjected to 16S rDNA (ribosomal deoxyribonucleic acid) gene sequencing identification, the sequence length is 1397 bp, and the sequence homology with the known Glutamicibacter soli is 99.93%. The strain is preserved in Institute of Microbiology, Chinese Academy of Sciences, and the preservation number is CGMCC No.34972. The invention provides intestinal symbiotic bacteria for enhancing baculovirus resistance of spodoptera frugiperda and application of the intestinal symbiotic bacteria, and provides an experimental basis for further revealing an interaction mechanism among a host, a pathogen and the symbiotic bacteria. And meanwhile, a technical basis is provided for realizing a comprehensive virus prevention and control strategy of targeted biological prevention and control and non-target beneficial insect protection of the lepidoptera pests.

The application discloses application of an enzyme in degrading ochratoxin A. The amino acid sequence of the enzyme comprises: (1) a sequence shown in SEQ ID NO. 1, or (2) an amino acid sequence obtained by substituting, deleting or adding one or at least two amino acid residues from the sequence in (1) and being identical or similar in function to the sequence in (1), or (3) an amino acid sequence having at least 90% sequence homology with the sequence in (1) or (2) and being identical or similar in function to the sequence in (1). The application first discovers that an enzyme derived from Thermomonas fusca has the function of degrading ochratoxin A, and catalyzes ochratoxin A to degrade into non-toxic substances, so as to be expected to be applied in degrading ochratoxin A, and the degradation rate is more than 90%, and the application has substantial application value and great significance in the aspect of biological detoxification of food and feed.

A method for producing PF1378A, which comprises a step for obtaining PF1378A by bringing one or more proteins selected from the group consisting of the following (1)-(4) into contact with PF1378B: (1) a protein comprising the amino acid sequence depicted in SEQ ID NO: 2, (2) an amino acid sequence having 70% or more sequence homology with respect to the amino acid sequence depicted in SEQ ID NO: 2, and (3) a protein comprising an amino acid sequence having 70% or more sequence homology with respect to the amino acid sequence depicted in SEQ ID NO: 1. (3) a protein encoded by the base sequence depicted in SEQ ID NO: 1; and (4) a protein encoded by a base sequence having a sequence homology of 70% or more with respect to the base sequence depicted in SEQ ID NO: 1, and having an activity of converting PF1378B into PF1378A.

The present application relates to the field of biotechnology and molecular diagnosis, and discloses a tsRNA detection primer composition, a kit, a method and an application, wherein the primer composition comprises a specific connecting primer for a tsRNA target, a CRISPR / Cas13a guide RNA, a signal probe and an RT-PCR primer. The kit and the method of the present application are based on the combination of a "linker-tailing" connecting reaction and a CRISPR / Cas13a system, and provide three modes of fluorescent detection, colorimetric detection and RT-PCR detection. Among them, the colorimetric detection mode innovatively introduces a Cas13a-mediated in-situ synthesis of a bimetallic nanoscale enzyme technology, and realizes double amplification of the signal through magnetic separation and nanoscale enzyme catalytic color development. The present application can effectively distinguish tsRNA and maternal tRNA with high sequence homology, has the advantages of strong specificity, high sensitivity, simple operation and visual results, and can be widely applied in the ultra-sensitive detection of tumor markers tsRNA and related scientific research and clinical auxiliary diagnosis.

Disclosed herein are fusion proteins comprising a truncated thrombostasin protein having at least 85% sequence homology to a thrombostasin protein, wherein the thrombostasin protein has a carboxy terminal deletion; and a fusion partner protein that is a non-thrombostasin protein. Further disclosed are vaccine compositions thrombostasin proteins having a comprising a carboxy terminal deletion, and methods for inhibiting a response to a thrombostasin protein in a host in need thereof, comprising the disclosed fusion proteins or vaccine compositions. Further disclosed are methods for the preparation of a fusion protein composition. 1005296120 20 24 20 36 26 30 M ay 2 02 4 A B S T R A C T 2 0 2 4 2 0 3 6 2 6 3 0 M a y 2 0 2 4 1 0 0 5 2 9 6 1 2 0

The present invention relates to a novel CRS fragment peptide with immune-enhancing activity and its uses, more specifically to a novel peptide consisting of the amino acid sequence of SEQ ID NO: 2 or an amino acid sequence having 95% or more sequence homology thereto, and its use as a vaccine adjuvant, an anticancer agent, and an antiviral composition. The peptide disclosed in the present invention is a CRS fragment disclosed for the first time in this specification, exhibiting anticancer activity, immune function enhancement, and antiviral activity.

The application discloses application of an enzyme in degradation of zearalenone. The amino acid sequence of the enzyme comprises: (1) a sequence shown in SEQ ID NO. 1, or (2) an amino acid sequence obtained by substituting, deleting or adding one or at least two amino acid residues from the sequence in (1) and identical or similar in function to the sequence in (1), or (3) an amino acid sequence with at least 90% sequence homology to the sequence in (1) or (2) and identical or similar in function to the sequence in (1). The application first discovers that an enzyme derived from Xylaria multiplex has the function of degrading zearalenone, and catalyzes the degradation of zearalenone into non-toxic substances, so as to be expected to be applied in degradation of zearalenone, the degradation rate is more than 90%, and the application has substantial application value and great significance in the aspect of biological detoxification of food and feed.

The invention relates to the field of biotechnology and molecular diagnosis, and discloses a tsRNA detection primer composition, a kit, a method and application, and the primer composition comprises a specific connection primer aiming at a tsRNA target, CRISPR / Cas13a guide RNA, a signal probe and an RT-PCR primer. According to the kit and the method, three modes of fluorescence detection, colorimetric detection and RT-PCR (Reverse Transcription-Polymerase Chain Reaction) detection are provided on the basis of the combination of'linker-tailing 'ligation reaction and a CRISPR / Cas13a system. According to the colorimetric detection mode, a Cas13a-mediated in-situ synthesis bimetallic nano-enzyme technology is innovatively introduced, and dual amplification of signals is realized through magnetic separation and nano-enzyme catalytic color development. The method can effectively distinguish tsRNA and female parent tRNA which are highly homologous in sequence, has the advantages of high specificity, high sensitivity, simplicity and convenience in operation and result visualization, and can be widely applied to ultra-sensitive detection of tumor marker tsRNA and related scientific research and clinical auxiliary diagnosis.

The application provides application of an artificial synthetic carrier RNA in nucleic acid detection, which is used for improving the sensitivity of exogenous nucleic acid detection, the nucleic acid sequence of the carrier RNA has high homology or cross-complementarity with the rRNA sequence of the endogenous host in the sample to be detected, and the rRNA sequence is the whole or partial sequence of 18S rRNA and / or 28S rRNA or an RNA sequence having at least 90% sequence homology with the whole or partial sequence of 18S rRNA and / or 28S rRNA. The new high-sensitivity exogenous nucleic acid detection technology provided by the application solves the key problems in the prior art, such as poor sample universality, large sequencing background interference in the existing carrier RNA use scheme and low nucleic acid detection sensitivity, significantly improves the effective proportion of trace RNA virus nucleic acid in the final sequencing library, and thus greatly improves the nucleic acid detection sensitivity of viruses and the like.

The application discloses a glycosyltransferase mutant and application thereof, and the mutant is obtained by single mutation or multiple mutations of amino acids at positions 58, 181 or 371 of the amino acid sequence shown in SEQ ID NO. 4 or single mutation or multiple mutations of amino acids at positions 54, 152, 241 or 272 of the amino acid sequence shown in SEQ ID NO. 6. The glycosyltransferase is rationally modified by homologous modeling and sequence homology analysis, and a glycosyltransferase mutant with obviously improved enzyme activity is screened. Compared with the wild type, the relative enzyme activity of the mutants UGTM1-3 and UGTM2-4 is respectively improved by 2.88 times and 3.60 times compared with that before modification, and the mutants have great industrial application potential.

The invention provides a novel nucleic acid polymerase which can be used for a single molecule sequencing technology. The polymerase belongs to a Phi29 polymerase family, but the sequence homology of the polymerase is lower than that of other reported polymerase for a sequencing technology by 60%. Through characterization identification, the polymerase shows good comprehensive performance in the aspects of thermal stability, extension capacity at normal temperature, extension proceeding, utilization capacity / chain substitution function catalytic kinetics for modified nucleotide substrates, electrolyte resistance and the like, and is suitable for a single-molecule sequencing technology based on a scheme of'sequencing while synthesizing '. The invention also provides a beneficial mutant strain of the wild type polymerase, which shows better dynamic characteristics and can improve the sequencing accuracy and flux.

This invention relates to a nucleic acid encoding human HGF and its applications. The nucleic acid comprises one or more open reading frames (ORFs), and the ORF nucleic acid sequence has at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, or 100% sequence homology with nucleic acid sequences selected from SEQ ID NO:21-31. This invention provides a nucleic acid encoding human HGF, nucleic acid constructs, vectors, cells, and drugs thereof, wherein the nucleic acid has a protein expression level superior to that of the native sequence.

The present application relates to a kind of biological species homology analysis systems based on protein / gene sequence data, belong to biological species homology analysis technical field, solve the problem of high cost and low efficiency of detection method in prior art.The system of the present application includes: data preprocessing module, for the obtained protein / gene original data is carried out data cleaning, obtain protein / gene sequence data;Data analysis module includes: protein sequence analysis unit, for according to the frequency of each amino acid in protein sequence, position and its physical and chemical properties, generate protein sequence numerical representation vector;Gene sequence analysis unit, for obtaining the optimal matching sequence in two groups of gene sequences;Species homology judging module, for according to protein sequence numerical representation vector / optimal matching sequence, the distance of each two groups of protein / gene sequence data is calculated, and according to the size of distance, the analysis of protein / gene sequence homology nearness.

The invention relates to a centromere sequence alignment method based on phylogenetic information and pairing editing distance, and belongs to the technical field of centromere sequence alignment. According to the algorithm provided by the invention, a collinearity relation is constructed by utilizing phylogenetic information of a satellite sequence and a pairing editing distance. According to the method, firstly, a conservative selection mode is adopted, and an editing distance (ED) threshold value is gradually increased until a collinear framework between two sequences covers more than 20% of the total number of monomers or 100 monomers; according to the method, an ordered chain with the highest comprehensive score is identified through DAGchainer software according to sequence homology and genome coordinates by integrating the physical distance between adjacent elements, the sequence and the E value score of BLAST, and a collinear satellite region is constructed. Then, a sliding window strategy is adopted, 20 collinear satellite pairs serve as a window, sub collinear satellite areas are recognized, and therefore the analysis precision is effectively improved, and local satellite collinear pairs are supplemented by widening an editing distance (ED) threshold value.

The present application relates to a kind of biological species homology analysis methods based on protein sequence data, solve the problem of high cost and low efficiency of detection method.The method of the present application comprises: obtaining the protein sequence of X group biological species, according to the occurrence frequency of various amino acids in each group of protein sequence and the physicochemical properties of each amino acid, generate the amino acid frequency information vector and the average value vector of amino acid physicochemical properties of X group protein sequence;According to the position information of 1~K letter amino acid in protein sequence, generate X group amino acid position probability vector;Dimension reduction is carried out to each group of amino acid position probability vector, and the dimension-reduced amino acid position probability vector is obtained;According to amino acid frequency information vector, amino acid physicochemical properties average value vector and dimension-reduced amino acid position probability vector, the distance of each two groups of protein sequence is calculated, and the distance is analyzed according to the size of protein sequence homology.

According to the invention, the EnzyForge AI deep learning screening method for novel enzyme molecule mining is constructed and provided for the first time. Aiming at the technical bottleneck that traditional enzyme screening depends on sequence homology, and low-homology enzymes with similar functions are difficult to effectively discover, the method creatively combines sequence homology search and structural similarity search, and takes a local catalytic key structural domain as a function judgment core; on the basis, local residue comparison is further carried out, and multi-dimensional evaluation is carried out on the activity, the catalytic potential and the stability of the candidate enzymes by combining a protein large language model, so that deep screening and sorting of the candidate enzymes are realized. Finally obtained protein is cloned to a pET-28a carrier, heterologous expression is realized in escherichia coli BL21 (DE3), a closed-loop process from computational prediction to structure screening to activity evaluation to experimental verification is constructed, and systematic and large-scale intelligent mining of lipase is realized.

The present invention relates to the field of allergens and more specifically to a novel isolated allergen from the species Cupressaceae shown to be a primary sensitizer for severe peach allergy mediated by the protein Pru p7. The allergen is homologous with Pru P7 in peach, a protein belonging to the Gibberelin Regulating Proteins (GRPs). The novel allergen also shares high sequence homology among other species from the Cupressaceae family and there is therefore also provided additional novel allergens in two other species of the Cupressaceae family. The novel allergens find use in the in vitro diagnosis of, treatment and / or prevention of Type 1 Cupressaceace pollen allergy and Cupressaceace pollen-associated food allergies.

The invention discloses a method for separating and identifying helichrysum psammophila rhizosphere growth-promoting bacteria and application thereof, and relates to the technical field of biologica.According to the technical key points, the helichrysum psammophila rhizosphere growth-promoting bacteria are screened through an abutilon ammophila culture medium, an organic phosphorus dissolving culture medium, an inorganic phosphorus dissolving culture medium, a CAS detection culture medium and a Salkowski colorimetric method, and the helichrysum psammophila rhizosphere growth-promoting bacteria are obtained; the growth promoting potential of the strain in the aspects of phosphorus solubilization, siderophore production, nitrogen fixation and the like is analyzed, a phylogenetic tree is constructed by adopting 16S rRNA sequence homology analysis to identify the strain, and finally, the growth promoting effect of the strain is verified by adopting an in-dish germination test of helichrysum psammophila and a potted helichrysum psammophila experiment. The bacillus sorola S31 provided by the invention can be used as a candidate growth-promoting rhizobacteria, and valuable strain resources are provided for the development of microbial agents.

This invention discloses a method for identifying medicinal rhubarb and tanguticum rhubarb based on differences in DNA fragment repeatability, belonging to the field of traditional Chinese medicine identification technology. The method includes the following steps: collecting three-year-old medicinal rhizome samples of the rhubarb to be tested; extracting total DNA; performing PCR amplification using specific primer pairs; sequencing the amplified products to obtain ITS2 and / or psbA-trnH sequences; comparing the sequenced sequences with a database; and identifying the sample as medicinal rhubarb (Rheum officinale) or tanguticum rhubarb (Rheum tanguticum) based on sequence homology. This invention significantly improves the accuracy and efficiency of identification by optimizing sample pretreatment conditions and improving the PCR amplification procedure, making it suitable for rapid and accurate identification of traditional Chinese medicinal materials.

Disclosed herein are a novel antibody and an antigen binding fragment thereof that targets CD30 to treat cancer, a chimeric antigen receptor, and a use thereof. The antibody, which binds specifically to CD30 that is highly expressed in cancer cells (specifically blood cancer), is very low in sequence homology with the CDR sequences of conventional CD30 targeting antibodies and thus has a unique sequence. Cells that express the chimeric antigen receptor including the anti-CD30 antibody or the antigen binding fragment responds to CD30-expressing positive cells to induce immune cell activity, thus finding advantageous applications as a CAR-immune cell product.

The invention discloses a kit for detecting specific early diagnosis of focal stage glomerulosclerosis, through innovative primer probe design and detection system optimization, the core advantage of the kit is that six miRNA markers, namely miR-125b, miR-186, miR-193a-3p, miR-17, miR-451 and miR-19b, which are verified by a multi-center queue are innovatively integrated; six groups of specific reverse transcription primers based on a stem-loop structure are successfully designed. According to the kit for specific early diagnosis of focal segmental glomerulosclerosis, aiming at the technical defects of low abundance, high sequence homology, poor multi-index detection compatibility and the like of focal segmental glomerulosclerosis (FSGS)-related miRNA, systematic optimization of a stem-loop reverse transcription method and a real-time fluorescent quantitative PCR (qPCR) two-step method is carried out, so that the specificity of the focal segmental glomerulosclerosis is improved, and the specificity of the focal segmental glomerulosclerosis is improved. The detection efficiency, the sensitivity and the specificity are obviously improved.

The invention provides application of an artificially synthesized carrier RNA (Ribonucleic Acid) for improving exogenous nucleic acid detection sensitivity in nucleic acid detection. A nucleic acid sequence of the carrier RNA and an rRNA sequence of an endogenous host of a to-be-detected sample have high homology or cross complementarity; the rRNA sequence is all or part of the sequence of 18S rRNA and / or 28S rRNA, or an RNA sequence having at least 90% of sequence homology with the all or part of the sequence of 18S rRNA and / or 28S rRNA. The novel exogenous nucleic acid high-sensitivity detection technology provided by the invention solves the key problems of poor sample universality of the existing method, large sequencing background interference, low nucleic acid detection sensitivity and the like in the existing vector RNA use scheme, so that the effective proportion of trace RNA virus nucleic acid in a final sequencing library is remarkably increased, and the detection sensitivity is high. Therefore, the detection sensitivity of nucleic acids such as viruses and the like is greatly improved.

The invention discloses a fluorescent quantitative PCR (Polymerase Chain Reaction) primer group, a kit and a method for detecting tomato neck and root rot germs and application. Belongs to the technical field of gene detection. Sequence homology comparison is carried out on the tomato neck and root rot pathogen and other fusarium oxysporum genomes, a specific site for detecting the tomato neck and root rot pathogen is determined, and the sequence is as shown in SEQ ID NO.19. The site is further utilized to design an amplification primer group, a kit and a detection method, a real-time fluorescent quantitative PCR detection system of the FORL is established, the system is good in specificity, high in sensitivity, strong in repeatability and high in accuracy, can detect genome DNA of the tomato neck and root rot bacteria, the sensitivity is 16.4 pg / mu L, the quantity of the bacteria in infected plants can be rapidly and accurately identified, and the method is suitable for popularization and application. And a scientific basis is provided for early prevention and treatment of tomato neck and root rot.

The application relates to a recombinant collagen protein targeting activated Frizzled-8 protein and a preparation method and application thereof, and belongs to the technical field of recombinant proteins. The recombinant collagen protein targeting activated Frizzled-8 protein has an amino acid sequence which comprises an amino acid sequence shown in any one of SEQ ID No. 1-SEQ ID No. 4 or an amino acid sequence with homology greater than or equal to 80% with any one of SEQ ID No. 1-SEQ ID No. 4. On the basis of realizing cell interstitial filling, the recombinant collagen protein can be combined with Frizzled-8 protein, and formation of the collagen protein is significantly promoted.