83 results about "Genetics genomics" patented technology

The invention discloses a lung squamous cell carcinoma drug discovery method and system based on disturbance response scanning analysis, and belongs to the field of biological medicines.The method comprises the steps that lung squamous cell carcinoma protein expression profile data is downloaded and preprocessed, a robust network module is constructed and multi-scale analysis and evaluation are conducted, and drug genomic data are obtained based on drug genomic data. Predicting the drug sensitivity of the patient by using machine learning, and generating a DRN through difference analysis of the drug sensitivity; predicting drug-target protein binding affinity in combination with deep learning, quantifying node sensitivity through a PRS technology, calculating a DPI disturbance score and ranking drug priorities; high-ranking drugs are screened through comprehensive sensitivity analysis and literature investigation; a drug for a lung squamous carcinoma cell line is screened out through a pre-experiment and is relocated. According to the framework developed by the invention, by integrating proteomics, pharmacogenomics and dynamic network analysis, systematic analysis is provided for relocation drug identification of lung squamous cell carcinoma, and good news is brought to treatment of lung squamous cell carcinoma.

The invention discloses a panax notoginseng MYB transcription factor gene PnMYB8, the nucleotide sequence of the gene PnMYB8 is as shown in SEQ ID NO: 1, an MYB transcription factor is encoded, molecular biology and functional genomics related technologies prove that the PnMYB8 gene has the capability of improving fungal infection resistance of plants, the PnMYB8 gene is constructed on a plant expression vector and transferred into tobacco for overexpression, and the yield of the panax notoginseng MYB transcription factor gene PnMYB8 is improved. Experimental results show that the resistance of the transgenic tobacco overexpressed with the PnMYB8 to the tryptophan destructor (Cylindrocarpon destructor), the fusarium latericum (Fusarium latericum), the fusarium equiseti (Fusarium equiseti) and the phoma herbarum (Phoma herbarum) is obviously enhanced, and the resistance of the transgenic tobacco overexpressed with the PnMYB8 to the tryptophan destructor (Fusarium latericum) is obviously enhanced.

The invention discloses a pig breeding screening method and system based on a body type database, and particularly relates to the technical field of pig breeding screening. The system comprises a multi-modal sign data acquisition and integration module for collecting and standardizing heterogeneous data sources such as phenotypic signs, genomics and environmental parameters of breeding pigs in real time, and a multi-dimensional biological characteristic modeling and analysis module for analyzing a nonlinear action mechanism among genetic markers, phenotypic characteristics and environmental factors, the genetic potential intelligent judgment module is used for quantitatively evaluating spatial expression potential and intergenerational transmission probability of genetic advantages of the breeding pigs through a deep learning algorithm; and the intelligent screening and dynamic monitoring module is used for establishing a multi-dimensional threshold dynamic regulation and control mechanism to realize accurate elimination decision and continuous optimization of a germplasm resource library. According to the method, the screening accuracy is remarkably improved by designing multi-source data fusion and online optimization capability, meanwhile, mistaken elimination is reduced through a redundant superior species temporary storage management module, and collaborative improvement of population genetic diversity and environmental adaptability is ensured.

The invention discloses Aspergillus sp. DLF18 and application thereof, and relates to the technical field of microorganisms, and the preservation number of the Aspergillus sp. DLF18 is CGMCC (China General Microbiological Culture Collection Center) NO.41737. The strain can effectively utilize rice straws and / or bagasse to produce humic acid, and the content of humic acid in each gram of fermentation supernatant after the rice straws are fermented is 16.5%. When the DLF18 fermentation product is applied to crops, the growth condition of the crops can be remarkably improved, and the humus content in soil is increased. Meanwhile, a metagenomics analysis result shows that compared with a control group, after the DLF18 is applied into the soil, the species beta diversity is remarkably changed, and the functional gene abundance and the inter-group difference are remarkably changed. Therefore, the DLF18 has a wide application prospect in the aspects of agricultural straw utilization and soil remediation.

The invention discloses application of a wheat gene TaGAMYB-like in improvement of plant salt tolerance, and belongs to the technical field of functional genomics. Wherein the nucleotide sequence of the wheat gene TaGAMYB-like is as shown in SEQ ID NO: 1, and the gene can improve the tolerance of plants to salt stress. The method has the beneficial effects that the wheat gene TaGAMYB-like is transformed into the model plant arabidopsis thaliana, the salt tolerance of the arabidopsis thaliana can be obviously improved, if the wheat gene TaGAMYB-like is transformed into plants such as wheat, rice, corn and cauliflower, new germplasm with the salt tolerance characteristic can be possibly obtained, and the new germplasm can be used as a new germplasm of the arabidopsis thaliana. The method is of great significance to cultivation of new varieties of excellent crops and wide application of the new varieties of the excellent crops in production.

The invention discloses a pseudo-ginseng bHLH transcription factor gene PnbHLH2, the nucleotide sequence of the pseudo-ginseng bHLH transcription factor gene PnbHLH2 is as shown in SEQ ID NO: 1, a bHLH transcription factor is encoded, molecular biology and functional genomics related technologies prove that the PnbHLH2 gene has the capability of improving fungal infection resistance of plants, the PnbHLH2 gene is constructed on a plant expression vector and transferred into tobacco for overexpression, and the yield of the pseudo-ginseng bHLH transcription factor gene PnbHLH2 is improved. Experimental results show that the resistance of the transgenic tobacco overexpressed with the PnbHLH2 to fusarium latericum, fusarium moniliforme, humicola fucoatra and cylindrocarpon destructor is obviously enhanced, and the resistance of the transgenic tobacco overexpressed with the PnbHLH2 to the fusarium latericum, the fusarium moniliforme, the humicola fucoatra and the cylindrocarpon destructor is obviously enhanced. The transgenic tobacco overexpressed with the PnbHLH2 has the advantages that the resistance of the transgenic tobacco overexpressed with the PnbHLH2 to the fusarium latericum, the fusarium moniliforme, the fusarium moniliforme and the cylindrocarpon destructor is obviously improved;

The invention discloses Penicillium chrysogenum DLS189 and application thereof, and relates to the technical field of microorganisms, and the preservation number of the Penicillium chrysogenum DLS189 is CGMCC (China General Microbiological Culture Collection Center) NO.41739. The invention further discloses a preparation method of the Penicillium chrysogenum DLS189. The strain can effectively utilize rice straws and / or bagasse to produce humic acid, and the content of humic acid in each gram of fermentation supernatant after the rice straws are fermented is 16.84%. When the DLS189 fermentation product is applied to crops, the growth condition of the crops can be remarkably improved, and the humus content in soil is increased. Meanwhile, a metagenomics analysis result shows that compared with a control group, after the DLS189 is applied into the soil, the species beta diversity is remarkably changed, and the functional gene abundance and the inter-group difference are remarkably changed. Therefore, the DLS189 has a wide application prospect in the aspects of agricultural straw utilization and soil remediation.

The invention relates to the technical field of crossing of bioinformatics, artificial intelligence and plant genomics, and discloses a plant single cell gene expression prediction method, system, device and medium. A plant single cell gene expression prediction model realizes dynamic feature fusion of a DNA sequence and chromatin accessibility signals through a gated cross attention mechanism; the problem that a traditional single-mode model cannot model regulation and control dynamic association is effectively solved, and the result interpretability is enhanced; a hybrid expert system and a load balancing design are adopted to significantly improve the recognition capability of the model for rare cell types, and a decoupling prediction head design supports efficient transfer learning; a DNA long sequence processing mechanism and nucleosome scale feature coding ensure cross-species compatibility; an end-to-end automatic process and a dynamic parameter optimization framework greatly improve the practicability; a'prediction-verification 'closed-loop support system can be constructed for molecular breeding, and high-precision and interpretable prediction of plant single-cell gene expression is realized.

The invention relates to the technical field of medical data analysis, in particular to a urinary system tumor big data analysis system which comprises a target kernel generation module, a kernel matrix calculation module, a parameter optimization module and a risk division module. According to the method, a target kernel matrix reflecting clinical prognosis differences is constructed and serves as an optimization reference, a radiomics and genomics feature kernel matrix is generated through hardware acceleration parallel computing, kernel function width parameters are dynamically iteratively updated based on an alignment degree numerical value so as to ensure that multi-modal feature distribution is highly matched with a prognosis label, and the accuracy of the multi-modal feature distribution is improved. A multi-dimensional feature space containing rich pathological information is constructed by combining a weighted fusion mechanism after centralization processing, so that a support vector machine is trained to determine a high-robustness decision boundary, and precise division of tumor risk levels is realized while high-dimensional data calculation delay is greatly reduced; and the reliability and timeliness of auxiliary diagnosis and treatment results in a complex pathological environment are effectively improved.

The invention relates to an application of a FaPDHE1alpha gene or an encoding protein thereof in prevention and control of strawberry gray mold, and belongs to the field of plant genetic engineering and the field of prevention and control of postharvest diseases of fruits and vegetables. The invention discloses a key positive regulation and control effect of FaPDHE1alpha in regulation and control of strawberry gray mold resistance. By constructing a 35S:: FaPDHE1alpha overexpression vector and a FaPDHE1alpha RNAi interference vector, according to transient gene overexpression and RNA interference silencing tests, it is verified that high expression of the FaPDHE1alpha gene can obviously enhance the resistance of strawberry fruits to botrytis cinerea, and silencing of the gene causes reduction of the disease resistance of the fruits. Mechanism studies show that after the FaPDHE1alpha gene is activated, mitochondrial energy metabolism and controlled ROS signal generation are promoted, antioxidant enzyme activity is enhanced, and defense related gene expression is induced, so that the disease resistance of fruits is improved. By means of functional genomics, the key function of the FaPDHE1alpha gene in prevention and control of gray mold after strawberry harvest is illuminated for the first time, and the FaPDHE1alpha gene can be used for screening disease-resistant breeding materials or developing biological prevention and control preparations for postharvest diseases and has important application value.

The invention discloses application of a wheat gene TaPHR1 in improvement of plant salt tolerance, and belongs to the technical field of functional genomics. Wherein the nucleotide sequence of the wheat gene TaPHR1 is as shown in SEQ ID NO: 2, the tolerance of a plant to salt stress can be improved through overexpression of the gene, and the plant is wheat or arabidopsis thaliana. The wheat gene TaPHR1 has the beneficial effects that the wheat gene TaPHR1 is transformed into a model plant arabidopsis thaliana, so that the salt tolerance of the arabidopsis thaliana can be obviously improved, if the wheat gene TaPHR1 is transformed into plants such as wheat, rice, corn and cauliflower, new germplasm with salt tolerance can be possibly obtained, and the new germplasm can be used as a new germplasm. The method is of great significance to cultivation of new varieties of excellent crops and wide application of the new varieties of the excellent crops in production.

The invention discloses a plant gene function analysis method (FAGEN), and belongs to the technical field of molecular genetics and functional genomics. The method comprises the following steps: constructing a target gene mutant by using a gene editing technology; hybridizing the mutant and the corresponding wild type as a common parent with a plurality of parents with different genetic backgrounds to construct a pair F1 group; an F2 group is obtained through selfing; and determining phenotypic data of target characters of F1 and F2 populations, and comparing phenotypic differences of filial generations of wild types and mutants under different genetic backgrounds to judge the functions of the target genes. The method has the advantages of simplicity in operation, flexibility in design, short construction period and low cost, and gene functions can be efficiently and accurately analyzed under various genetic backgrounds.

The invention relates to the technical field of genomics and metabonomics, in particular to a method for analyzing intestinal health of crisp tilapia mossambica. The tilapia mossambica intestinal health judgment model is established by dynamically monitoring tilapia mossambica muscle texture and intestinal morphology through high time resolution, analyzing intestinal health three-dimensional indexes (tissue morphology-flora structure-host gene expression) and serum metabolism response after embrittlement succeeds, and combining cross-omics data association analysis. The change condition of the muscle texture can be known more timely, a more accurate basis is provided for adjustment of a feeding strategy, and the stability of the fish meat quality can be improved. The intestinal health is evaluated from three dimensions of histomorphology, flora structure and host gene expression, so that the health condition of the intestinal tract can be known more comprehensively, and more comprehensive reference is provided for optimization of a feeding method. The correlation between different omics data can be deeply analyzed, the physiological and metabolic mechanisms of the fish body are disclosed, and deeper theoretical support is provided for optimization of a feeding method.

This invention discloses a multi-scale prediction method and system for the maximum growth rate of soil microorganisms based on genomic features, involving the interdisciplinary fields of microbial ecology and genomics. The method includes: genomic data preparation, core genomic feature extraction, single-scale growth rate prediction, community-scale extrapolation, and result verification and output. The system includes a genomic data processing unit, a core feature extraction module, a growth rate prediction model, a community-scale extrapolation module, and a result visualization and verification unit. This invention overcomes the limitations of traditional culture methods and in-situ measurement methods, achieving high-throughput and accurate prediction of the growth rates of culturable and uncultured microorganisms, and solving the problems of low coverage, fragmented scales, and high costs of existing technologies. It is applicable to soil microbial function assessment, carbon cycle model parameterization, and ecosystem management.

This invention provides a method for storing mango seeds, belonging to the field of seed preservation technology. The method involves cutting off mango fruits with embryos, along with their pedicels and branches, 77-84 days after fruit set. A mixture of pyraclostrobin, lambda-cyhalothrin, and chlorothalonil is sprayed on the fruit, pedicels, and branches. After drying, styrene-butadiene latex is applied to the pedicels and branches. Once the latex dries, a mixture of guanidine-copper acetate and benzoyl-pyraclostrobin is sprayed on the fruit, pedicels, and branches again. After drying, the mango fruits with petioles and branches are placed in a sealed box and stored at 10-14°C. The mango variety used is the Golden Phoenix mango. This invention increases the storage period of Golden Phoenix mango seeds from 1.5 months to 5 months, which is of great significance for the genomics and cell biology research of Golden Phoenix mangoes.

The invention belongs to the technical field of fish genetic breeding, and relates to a method for screening hemorrhagic disease resistant grass carp and application of the method in hemorrhagic disease resistant breeding of grass carp. Based on a genomics rapid breeding technology, a batch of grass carps capable of resisting the hemorrhagic disease are accurately screened out through the steps of constructing a grass carp experimental group, identifying phenotypes through artificial infection of grass carp hemorrhagic disease viruses, analyzing and identifying genotypes through high-throughput sequencing, carrying out associated screening on SNP sites related to the hemorrhagic disease and the like. The method realizes efficient breeding in a breeding cycle of the grass carp, has the advantages of high efficiency, low cost, genetic stability and the like, can effectively solve the breeding loss caused by hemorrhagic disease of the grass carp, and assists high-quality and green development of aquaculture industry in China.

This invention discloses the application of the wheat gene TaPSS2 in improving plant drought resistance, belonging to the field of functional genomics. The nucleotide sequence of the wheat gene TaPSS2 is shown in SEQ ID NO: 2. Transforming this gene into the model plant Arabidopsis thaliana can significantly improve the drought resistance of Arabidopsis. If the wheat gene TaPSS2 is transformed into wheat, rice, maize, cauliflower, and other plants, it is possible to obtain new germplasm with drought-resistant characteristics, which is of great significance for the breeding of superior crop varieties and their widespread application in production.

The invention relates to the technical field of biology, in particular to application of an RhUNE10 gene to regulation and control of root cancer resistance of Chinese roses and a regulation and control method. The nucleotide sequence of the RhUNE10 gene is as shown in SEQ ID NO. 1. The application mode is that the RhUNE10 gene is silenced and knocked out, or the expression of the protein coded by the RhUNE10 gene is inhibited; and the amino acid sequence of the protein coded by the RhUNE10 gene is as shown in SEQ ID NO. 3. After the RhUNE10 gene is silenced, the resistance of the Chinese rose to agrobacterium tumefaciens is remarkably reduced, which indicates that the RhUNE10 gene has important biological functions, is beneficial to promoting Chinese rose functional genomics research, gene editing breeding and transgenic breeding research, excavates and cultivates new varieties of high-resistance Chinese roses, and has wide application prospects. And a certain theoretical basis and key gene resources are provided for comprehensive prevention and treatment of root cancer and cultivation of a new variety of Chinese rose.

The invention discloses an application of a gene GmSW6 in regulation and control of plant grain weight and quality. GWAS is developed by using genotype and hundred-grain weight phenotype data, haplotype analysis, gene expression profile data and homologous gene function annotation are combined, and the grain weight related gene GmSW6 is successfully cloned by virtue of research means of molecular biology and comparative genomics. The function of the gene GmSW6 in regulating and controlling the plant grain weight is disclosed for the first time by utilizing a CRISPR-Cas9 system, and the target of increasing the hundred-grain weight of the plant is possibly achieved by knocking out a coding sequence of the GmSW6 gene or silencing the coding gene or reducing the expression level of the coding gene. Meanwhile, by introducing the gene into a target plant or over-expressing the gene in the plant, the grain weight of the plant can be regulated and controlled, the grain weight of the plant can be reduced, but the per unit yield can be increased under the condition, an important gene resource is provided for plant molecular breeding, and a new choice is provided for high yield of the plant.

The invention relates to the technical field of medicines, and discloses a bifidobacterium longum strain GZBAI 01 and application thereof in degradation of benzoic acid and prevention or treatment of colorectal cancer. The strain is separated from faeces of healthy people, genomics identification is carried out, the metabolic profile of the strain is measured through metabonomics, the strain can convert benzoic acid with carcinogenic risk into protocatechuic acid with antitumor activity through unique metabolic capability, meanwhile, the function of regulatory T cells (Treg) in tumor drainage lymph nodes can be inhibited, differentiation of CD8 + T cells is promoted, and the tumor drainage lymph nodes can be inhibited. Therefore, the colorectal cancer is prevented through double ways of eliminating cancerogen and generating therapeutic agents. A colorectal cancer mouse model and a subcutaneous tumor mouse model verify that the strain can significantly reduce the number and volume of tumors, and shows a benzoic acid conversion effect superior to that of other strains in vivo and in vitro, and a new way is provided for prevention and treatment of colorectal cancer.

This invention belongs to the fields of biotechnology and genomics, and relates to a method and kit for capturing plant chromatin conformation. The method includes the following steps: (1) cross-linking and fixing the chromatin of a plant sample; (2) digesting the fixed chromatin using an enzyme digestion combination containing restriction endonuclease AluI and restriction endonuclease HypCh4V; (3) ligating the enzyme fragments generated in step (2) at spatially adjacent locations to form ligation products; (4) enriching the ligation products and constructing sequencing libraries using the enriched ligation products. This invention uses a combination of AluI and HypCh4V enzymes to replace traditional single-enzyme digestion, which significantly improves data resolution while reducing technical bias. It can achieve chromatin interaction mapping at a resolution of 500 bp in plants and has broad application prospects in the study of fine tissue characteristics at the single-gene level in plants.

This invention discloses a strain of Aspergillus sp., DLF18, and its applications, belonging to the field of microbial technology, with accession number CGMCC NO.41737. This strain can effectively utilize rice straw and / or sugarcane bagasse to produce humic acid, with a humic acid content of 16.5% per gram of fermentation supernatant after rice straw fermentation. Applying the fermentation product of DLF18 to crops can significantly improve crop growth and increase the humic content in the soil. Simultaneously, metagenomic analysis results show that, compared with the control group, the application of DLF18 to the soil significantly altered species β-diversity, functional gene abundance, and inter-group differences. These findings indicate that DLF18 has broad application prospects in agricultural straw utilization and soil remediation.

The invention discloses a traditional Chinese medicine composition for preventing and treating avian pathogenic escherichia coli as well as a preparation method and application thereof, and relates to the technical field of veterinary drugs. According to the invention, screening is carried out through subtraction genomics and reverse network pharmacology, and based on the compatibility theory of monarch, minister, assistant and guide and the traditional Chinese veterinary medicine and pharmacy differentiation theory, a traditional Chinese medicine composition, namely the radix angelicae and fructus forsythiae intestine health formula, for preventing and treating avian pathogenic escherichia coli is formed. According to the traditional Chinese medicine composition, fructus forsythiae and radix angelicae are used as monarch drugs, radix isatidis and clinopodium polycephalum are used as ministerial drugs, fructus aurantii immaturus, mint and plantain herb are used as adjuvant drugs, and liquorice is used as a conductant drug, so that the traditional Chinese medicine composition can inhibit reproduction of avian pathogenic escherichia coli, destroy formation of biological membranes of the avian pathogenic escherichia coli and relieve pathological injuries of diseased poultry caused by APEC; the traditional Chinese medicine composition has a good treatment effect on avian pathogenic escherichia coli.

The invention discloses a pharmacogenomics report generation method and device, a medium and a product, and relates to the field. The method comprises the following steps: in response to the completion of a drug genome experiment of a first object, obtaining experimental result data of the drug genome experiment, and determining experimental result interpretation data based on a first drug gene association database; inputting the experimental result interpretation data, the object attribute information corresponding to the first object, the medical record information and the first medicine information into a pre-trained medication risk assessment model to obtain a medication risk assessment result of the first object; and generating a pharmacogenomics report of the first object based on the medication risk assessment result, the experimental result interpretation data, the object attribute information, the medical record information and the first drug information. According to the technical scheme of the invention, the problems of high threshold and low efficiency of the existing pharmacogenomics report generation are solved, the pharmacogenomics report can be automatically generated, the accuracy of the report content is improved, and the report generation cost is reduced.

The invention discloses a method for screening an anti-monochamus alternatus clonal pinus massoniana key gene based on genomics as well as an obtained gene and application thereof, and relates to the technical field of plant genetic engineering. The invention discloses a genomics-based method for screening an anti-monochamus alternatus clonal pinus massoniana key gene. The method comprises the following steps: screening a pinus massoniana anti-monochamus alternatus differential gene through a transcriptome; the masson pine anti-monochamus alternatus differential metabolites are screened out through the metabolome; according to a metabolome differential metabolite analysis result and in combination with a transcriptome differential gene analysis result, joint analysis is performed to construct an interaction network diagram, the pinus massoniana anti-monochamus alternatus key gene PmFLS is screened out, an overexpression strain of the pinus massoniana anti-monochamus alternatus key gene PmFLS is constructed, and the pinus massoniana anti-monochamus alternatus key gene PmFLS is obtained. Finally, a transgenic arabidopsis thaliana transgenic line with increased leaf area, advanced bolting time, increased total flavone content and increased FLS enzyme activity is obtained, and powerful support is provided for gene improvement of masson pine.

The application belongs to the field of microbial molecule detection, and discloses a multiple qPCR kit and method for simultaneously detecting multiple listeria based on new molecular targets. The application designs corresponding primers and probes based on specific gene fragments of each listeria obtained by genomics mining, establishes a multiple qPCR method, and assembles the method into a portable kit. The multiple qPCR primer probe set of the application has high specificity, and only the six target bacteria of Listeria monocytogenes, Listeria innocua, Listeria ivanovii, Listeria welshimeri, Listeria seeligeri and Listeria grayi appear fluorescence signals in the specific fluorescence signal channel, so that the detection result is more accurate. The multiple qPCR kit of the application can complete sample detection within 14 hours, wherein the whole qPCR process only needs about 1 hour, the detection efficiency is high, the detection cost is low, and the kit is particularly suitable for large-scale sample investigation and actual detection, and the detection sensitivity can be as low as 1.0-11.1 CFU / Test.

The invention discloses a genetic transformation method based on pear somatic embryogenesis, and belongs to the technical field of genetic engineering and molecular breeding. In order to solve the problems that existing pear genetic transformation is low in transformation efficiency, high in chimera rate, long in period, difficult to obtain gene editing regeneration plants and the like, the invention provides two paths: 1, immature zygotic embryos 30-60 days after pear flowering are taken as explants, resistant plants are directly induced through a somatic embryo regeneration path, and the transformation efficiency within 40-60 days reaches 73.91%; secondly, the embryogenic callus with the consistent genetic background is used as an explant for infection, the embryogenic property is maintained and the proliferation efficiency is improved by optimizing a culture medium formula, and a resistant plant with the consistent genetic background can be obtained within 30-50 days. The method overcomes the defect that regeneration plants are difficult to obtain through existing callus transformation, remarkably shortens the breeding period, is high in transformation efficiency, low in chimera rate and controllable in genetic background consistency, and provides technical support for pear functional genomics research and molecular breeding.

This invention belongs to the technical field of diabetes diagnosis, specifically the use of reagents for detecting intestinal microorganisms in the preparation of diabetic diagnostic preparations and kits. Through metagenomics and metabolomics, key biomarkers, including key differential gut microorganisms and key differential fecal metabolites, will be screened. Its clinical value lies in the eventual identification of a multi-omics classifier that can distinguish adult-onset T1D from healthy controls (HCs) and T2D. This will facilitate the precise diagnosis and typing of adult-onset T1D and provide novel diagnostic and therapeutic targets.

The application discloses a high-precision genotype reference panel of Cervus nippon and a filling method thereof, and belongs to the technical field of animal genomics. The method comprises the following steps: collecting blood samples of Cervus nippon with an age of 24 months or above, performing 5X resequencing, and obtaining genotyping data; using Beagle software to perform self-filling on the resequencing data, and generating a reference panel; using Plink software to perform quality control on the reference panel data; setting a DR 2 filtering threshold value as 0.95, filtering chip data of a target population; dividing sites into five intervals according to minor allele frequencies, and filling in sequence according to intervals. Through the specific implementable technical steps, the application solves the technical problem of poor filling effect of low-frequency variant sites in Cervus nippon genotype filling, and provides a low-cost and efficient genotyping scheme for Cervus nippon genomic breeding.

The invention discloses a rape cold-resistant gene and application thereof, and belongs to the technical field of gene engineering. Four rape specific lone genes (BnaOGs) and 2859 brassica specific genes (BSGs) are identified through comparative genomics and multi-database artificial verification by combining UniProt-KB, Nrdb and the like, and the characteristics of short sequence, high GC content, no transcription factor and the like are found. Aiming at a BSG gene BnaA03G0334200ZS, a CDS sequence of the BSG gene BnaA03G0334200ZS is cloned, a pEGOEP35S-H overexpression vector containing a 35S strong promoter and a hygromycin selection marker is constructed, and a positive plant is obtained through agrobacterium-mediated transformation of brassica napus Westar. Functional verification shows that overexpression of the gene remarkably enhances the cold resistance of the rape, plant damage is slight at low temperature, the relative conductivity and MDA content are reduced, and the activities of SOD, POD and CAT are improved. The invention provides a new target and technical support for cultivation of cold-resistant rape varieties, and has a wide application prospect.