14 results about "Genetics genomics" patented technology

This invention discloses a multi-scale prediction method and system for the maximum growth rate of soil microorganisms based on genomic features, involving the interdisciplinary fields of microbial ecology and genomics. The method includes: genomic data preparation, core genomic feature extraction, single-scale growth rate prediction, community-scale extrapolation, and result verification and output. The system includes a genomic data processing unit, a core feature extraction module, a growth rate prediction model, a community-scale extrapolation module, and a result visualization and verification unit. This invention overcomes the limitations of traditional culture methods and in-situ measurement methods, achieving high-throughput and accurate prediction of the growth rates of culturable and uncultured microorganisms, and solving the problems of low coverage, fragmented scales, and high costs of existing technologies. It is applicable to soil microbial function assessment, carbon cycle model parameterization, and ecosystem management.

This invention provides a method for storing mango seeds, belonging to the field of seed preservation technology. The method involves cutting off mango fruits with embryos, along with their pedicels and branches, 77-84 days after fruit set. A mixture of pyraclostrobin, lambda-cyhalothrin, and chlorothalonil is sprayed on the fruit, pedicels, and branches. After drying, styrene-butadiene latex is applied to the pedicels and branches. Once the latex dries, a mixture of guanidine-copper acetate and benzoyl-pyraclostrobin is sprayed on the fruit, pedicels, and branches again. After drying, the mango fruits with petioles and branches are placed in a sealed box and stored at 10-14°C. The mango variety used is the Golden Phoenix mango. This invention increases the storage period of Golden Phoenix mango seeds from 1.5 months to 5 months, which is of great significance for the genomics and cell biology research of Golden Phoenix mangoes.

This invention discloses the application of the wheat gene TaPSS2 in improving plant drought resistance, belonging to the field of functional genomics. The nucleotide sequence of the wheat gene TaPSS2 is shown in SEQ ID NO: 2. Transforming this gene into the model plant Arabidopsis thaliana can significantly improve the drought resistance of Arabidopsis. If the wheat gene TaPSS2 is transformed into wheat, rice, maize, cauliflower, and other plants, it is possible to obtain new germplasm with drought-resistant characteristics, which is of great significance for the breeding of superior crop varieties and their widespread application in production.

This invention belongs to the fields of biotechnology and genomics, and relates to a method and kit for capturing plant chromatin conformation. The method includes the following steps: (1) cross-linking and fixing the chromatin of a plant sample; (2) digesting the fixed chromatin using an enzyme digestion combination containing restriction endonuclease AluI and restriction endonuclease HypCh4V; (3) ligating the enzyme fragments generated in step (2) at spatially adjacent locations to form ligation products; (4) enriching the ligation products and constructing sequencing libraries using the enriched ligation products. This invention uses a combination of AluI and HypCh4V enzymes to replace traditional single-enzyme digestion, which significantly improves data resolution while reducing technical bias. It can achieve chromatin interaction mapping at a resolution of 500 bp in plants and has broad application prospects in the study of fine tissue characteristics at the single-gene level in plants.

This invention discloses a strain of Aspergillus sp., DLF18, and its applications, belonging to the field of microbial technology, with accession number CGMCC NO.41737. This strain can effectively utilize rice straw and / or sugarcane bagasse to produce humic acid, with a humic acid content of 16.5% per gram of fermentation supernatant after rice straw fermentation. Applying the fermentation product of DLF18 to crops can significantly improve crop growth and increase the humic content in the soil. Simultaneously, metagenomic analysis results show that, compared with the control group, the application of DLF18 to the soil significantly altered species β-diversity, functional gene abundance, and inter-group differences. These findings indicate that DLF18 has broad application prospects in agricultural straw utilization and soil remediation.

The application discloses a high-precision genotype reference panel of Cervus nippon and a filling method thereof, and belongs to the technical field of animal genomics. The method comprises the following steps: collecting blood samples of Cervus nippon with an age of 24 months or above, performing 5X resequencing, and obtaining genotyping data; using Beagle software to perform self-filling on the resequencing data, and generating a reference panel; using Plink software to perform quality control on the reference panel data; setting a DR 2 filtering threshold value as 0.95, filtering chip data of a target population; dividing sites into five intervals according to minor allele frequencies, and filling in sequence according to intervals. Through the specific implementable technical steps, the application solves the technical problem of poor filling effect of low-frequency variant sites in Cervus nippon genotype filling, and provides a low-cost and efficient genotyping scheme for Cervus nippon genomic breeding.

This invention discloses a Panax notoginseng MYB transcription factor gene. PnMYB34 Its nucleotide sequence is as described in SEQ ID NO:1, encoding the MYB transcription factor. This invention confirms through molecular biology and functional genomics related technologies. PnMYB34 The gene has the ability to enhance the plant's resistance to viral infection, and the present invention will... PnMYB34 Genes were constructed into plant expression vectors and overexpressed in Panax notoginseng and tobacco, respectively. Experimental results showed that overexpression... PnMYB34 Genetically modified Panax notoginseng against Panax notoginseng A virus (GMO) Panax notoginseng virus A The resistance to ) was significantly enhanced, and overexpression PnMYB34 Genetically modified tobacco is susceptible to tobacco mosaic virus (BMP). Tobacco mosaic virus Its resistance was also significantly enhanced.

The application discloses a plant phenotype analysis intelligent agent system based on a large language model. The system adopts a star topological structure, takes a planning intelligent agent as a hub, and is provided with special intelligent agents for vision, model fine tuning, code generation, GWAS analysis and paper writing, so that an overall process from multi-scale plant image processing, zero sample plant image segmentation, plant three-dimensional reconstruction and point cloud segmentation, neural network model optimization, visual chart drawing, genotype-phenotype analysis to scientific research paper writing is realized, and a one-stop intelligent solution is provided for accelerating high-throughput phenomics and genomics correlation research.

This invention belongs to the field of plant genetic engineering technology, specifically relating to the application of the wheat TaMRS2-16 protein in regulating photosynthetic rate. The purpose of this invention is to provide a novel use for the wheat TaMRS2-16 gene, namely, its application in regulating wheat photosynthetic rate. Through functional genomics, this invention, for the first time, clarifies the positive regulatory role of the TaMRS2-16 gene in wheat photosynthetic rate, providing new target genes and molecular markers for molecular breeding of high-efficiency wheat.

The present application relates to the field of plant molecular biology and genetic engineering technology, and particularly relates to a transient expression method of orchid plants and application. The method provided by the present application successfully breaks through the infection barrier of the thick cuticle of orchid plants by optimizing the core parameters of vacuum-assisted Agrobacterium infection, screening the infection strains and optimizing the composition of the infection solution. The high activity of the leaf cells can be maintained for more than 9 days after infection, and the exogenous genes can realize stable and efficient expression. The method provided by the present application is simple to operate, does not require special instruments and equipment, is convenient to obtain materials, is not limited by the flowering season, can be carried out throughout the year, and the complete experimental cycle can be completed within 7 days. The present application effectively solves the long-standing technical problems in the field of genetic transformation of orchid plants, and provides an efficient and convenient gene function transient verification technology platform for the functional genomics research, gene function verification, promoter activity detection and precise molecular breeding of orchid and other orchid plants.

The application discloses a genetic transformation method of Robinia pseudoacacia hairy roots mediated by Agrobacterium rhizogenes, and belongs to the technical field of plant genetic engineering and molecular breeding. The method comprises the following steps: preparing an Agrobacterium rhizogenes infection solution carrying a target gene; taking a Robinia pseudoacacia seedling, removing the root at the root stem connection to obtain an explant; immersing the wound of the explant into the infection solution to perform infection; subsequently, directly planting the infected explant into a substrate to perform co-culture and root induction until the transgenic hairy roots are formed at the wound. The method provided by the application completely abandons aseptic tissue culture and a complicated plant regeneration process, and realizes genetic transformation of Robinia pseudoacacia under non-aseptic and non-detached conditions. The method can shorten the transformation period from several months to 3-4 weeks, and the positive transformation rate can be stabilized to be more than 60%. The method is simple in operation, low in cost, efficient and stable, and can provide important technical support for functional genomics research, genetic improvement and molecular breeding of Robinia pseudoacacia.

The application discloses a genetic engineering method for creating early-flowering and yield-increasing rice, and belongs to the technical field of plant genetic engineering. EFHP The PACMAD group-specific MADS gene is identified by a comparative genomics method EFHP The gene is introduced into a main rice variety and overexpressed, so that the flowering time of the rice can be significantly regulated, the rice plants can bloom about 7 days in advance, the biomass and yield are increased, and the yield per plant is increased by 23-35%. EFHP The gene has a double function of simultaneously regulating the flowering time and yield of the rice, solves the technical problem that early flowering and yield increase are difficult to be simultaneously improved, and provides new gene resources and breeding materials for rice molecular breeding.

The application discloses a method for constructing a large fragment deletion mutant library of Magnaporthe oryzae, and belongs to the field of agricultural biotechnology.The method comprises the step of adding DEB to the protoplast of the Magnaporthe oryzae for culture and inducing the production of conidia.The application utilizes the chemical mutagen DEB to obtain the Magnaporthe oryzae mutant at a high frequency on a whole genome scale, and provides a new technical path for the functional genomics research of the Magnaporthe oryzae.The method provided by the application is helpful to systematically analyze the pathogenic mechanism, the infection process and the environmental adaptation regulation network of the Magnaporthe oryzae, and provides support for mining new disease-resistant targets and disease-resistant mechanisms.The method has the advantages of high efficiency, low cost and short cycle, and has wide application potential in the genetic research of plant pathogenic fungi.

This invention discloses a method for preparing a 10K SNP liquid-phase microarray for commercial pig breeds and its application, belonging to the field of molecular breeding and genomics technology. Based on representative samples from the core breeding population of Chinese commercial pigs, this invention employs liquid-phase probe capture technology to develop a high-performance pig genome SNP genotyping microarray containing 10,055 rigorously screened high-quality SNP loci. The probes are 110 bp in length, with a GC content of 30%–70% and ≤5 homologous regions. Each locus is captured using two probes with 60%–70% overlap for dual targeting, significantly improving hybridization efficiency and anti-interference ability, and enabling stable detection of low-quality DNA samples. Simultaneously, this invention sets dual quality control standards to ensure genotyping accuracy. Verification shows that the detection rate of this microarray ranges from 98.17% to 99.602%, with an average detection rate of 99.161%. This invention has significant value for improving the breeding efficiency of commercial pigs in my country.