The invention provides a specific dual-targeting antibody as well as a preparation method and application thereof. The specific dual-targeting antibody comprises a first antigen binding structural domain specifically bonded with human CD3, and a second antigen binding structural domain specifically bonded with a cMet antigen, and is capable of specifically identifying and bonding tumor cells with overexpression of the cMet antigen, wherein the tumor cells are of liver cancer, other cMet-positive tumor diseases and the like; furthermore, the specific dual-targeting antibody is also capable of bonding a T cell receptor, and mediates T cells to play a killing role, thus clearing cMet infection; therefore, the specific dual-targeting antibody has a great application value in diagnosis, treatment, prevention and detection of cMet-related diseases. The invention also provides microcircular deoxyribonucleic acid (DNA) encoding the nucleotide sequence of the specific dual-targeting antibody. The specific dual-targeting antibody and the microcircular DNA which are provided by the invention can be used for preparing antibody-based pharmaceutical compositions or cMet-positive tumor cell diagnostic reagents.