84 results about "MYB" patented technology

The invention discloses a coumarin synthetic gene in black bamboo and application thereof, and belongs to the technical field of molecular biology. The invention provides transcription factors PnMYB4 and PnMYB4-1 for promoting synthesis of coumarin in black bamboos and application of the transcription factors PnMYB4 and PnMYB4-1. The PnMYB4 and the PnMYB4-1 belong to an MYB transcription factor family, the length of a complete coding frame of the PnMYB4 is 762 basic groups, and the coded protein contains 253 amino acids; the length of a complete coding frame of the PnMYB4-1 is 744 basic groups, and the coded protein contains 253 amino acids. The coumarin substances in arabidopsis thaliana, which are respectively overexpressed by PnMYB4 and PnMYB4-1, are obviously increased, and in addition, the expression levels of coumarin synthetic genes such as AtPAL1, At4CL1 and AtC4H in overexpressed plants are obviously increased.

The invention discloses a corn ZmMYBR37 gene and an application of the corn ZmMYBR37 gene in regulating and controlling the size of corn kernels. The nucleotide sequence of the ZmMYBR37 gene is as shown in SEQ ID No: 1 in a sequence table. A mutant material with small grains and reduced hundred-grain weight is separated from a corn EMS mutant library, and a mutation site occurs on a function unknown gene ZmMYBR37, so that protein translation is terminated in advance. The information analysis finds that the gene coding protein has a conservative MYB structural domain, and the gene coding protein is speculated to belong to an MYB transcription factor family. Gene expression analysis finds that the gene is high in constitutive expression in corn and high in expression level in leaves and embryos. The corn material overexpressed with the ZmMYBR37 has the advantages that the grain size is increased, and the hundred-grain weight is obviously increased, so that the ZmMYBR37 has the potential of increasing the corn yield.

The invention discloses application of a scutellaria baicalensis SbMYB8 gene in promotion of anthocyanin synthesis, the application comprises the steps that the SbMYB8 gene is constructed to a plant expression vector and introduced into plant cells to promote synthesis of plant anthocyanin, and the nucleotide sequence of the SbMYB8 gene is shown as SEQ ID NO: 1, or the amino acid sequence coded by the SbMYB8 gene is shown as SEQ ID NO: 2. The MYB transcription factor SbMYB8 gene with the function of promoting anthocyanin synthesis is cloned and identified from the scutellaria baicalensis for the first time, and the blank of a transcription regulation mechanism for regulating anthocyanin metabolism in the scutellaria baicalensis is filled; the SbMYB8 gene can significantly promote accumulation of anthocyanin in various plants, shows good heterologous expression stability and adaptability, and has wide cross-species application potential.

The invention discloses an application of a gene OsRNM5 with a Myb structural domain. The cDNA (complementary deoxyribonucleic acid) sequence of the OsRNM5 is SEQ ID NO.1, and the amino acid sequence coded by the OsRNM5 is SEQ ID NO.2. Field yield measurement shows that compared with a wild type WYJ7, the plant height of an OsRNM5 overexpression material is reduced, the yield of a single plant is reduced, and the plant height of an OsRNM5 knockout material is increased, and the yield of the single plant is increased. By knocking out or silencing the OsRNM5 gene in the rice, the nitrogen fertilizer utilization efficiency and yield of the rice can be improved.

The invention provides a honeysuckle MYB gene family transcription factor 13476. A honeysuckle HQT2 gene, an HQT2 gene promoter and a transcription factor 13476 are cloned respectively, and a yeast one-hybrid experiment proves that 13476 can directly interact with the HQT2 promoter. Furthermore, the regulation of the 13476 protein on the transcriptional activity of the HQT2 gene is analyzed by using a dual luciferase system in tobacco, and the 13476 is proved to have an inhibiting effect on the promoter of the HQT2. Tobacco transient transformation results show that the content of chlorogenic acid in plant cells can be increased by 30-40% through heterologous transient expression of the LjHQT2 gene, and the content of chlorogenic acid reaches the level of 1.59 mg / g. The RNAi method is adopted, molecules aiming at Lj13476 are transiently expressed in honeysuckle, the content of chlorogenic acid can be increased by 10%-20%, the content of isochlorogenic acid A can be increased by 5%-30%, and the content of isochlorogenic acid C can be increased by 7%-20%. The interaction mechanism of the honeysuckle HQT2 gene and the transcription factor 13476 is studied, a certain basis is provided for the regulation mechanism of chlorogenic acid, and the HQT2 gene and the transcription factor 13476 are of great significance for breeding high-quality honeysuckle in the future.

The invention provides application of an MsMYBS3 gene or a biological material thereof in regulation and control of plant cold resistance, and belongs to the technical field of plant genetic engineering. The amino acid sequence coded by the MsMYBS3 gene disclosed by the invention is as shown in SEQ ID NO. 4. The invention provides an application of a medicago sativa MYB transcription factor gene MsMYBS3 in the aspect of regulating and controlling the cold resistance of medicago sativa. A gene editing technology is adopted to directionally change the development and the cold resistance of the medicago sativa. The cold resistance of an MsMYBS3 gene editing strain constructed by the invention is reduced, and the MsMYBS3 gene has the effect of regulating and controlling the cold resistance of alfalfa, so that a theoretical basis and a candidate gene are provided for cultivating new varieties of high-resistance leguminous forage, and the application of alfalfa in production is promoted.

The invention relates to the technical field of genetic engineering and molecular breeding, in particular to a ramie MYB transcription factor and application thereof. The MYB transcription factor is BnMYB6, the amino acid sequence of the MYB transcription factor is as shown in SEQ ID NO.1, and the gene sequence for coding the amino acid of the MYB transcription factor is as shown in SEQ ID NO.2. The transcription factor protein BnMYB6 can specifically and negatively regulate the biosynthesis of plant lignin, and has application value in the aspects of inhibiting the synthesis of plant lignin, promoting the growth and development of a root system and maintaining related traits such as the activity of the root system under drought stress.

The invention discloses application of apple MdMYB41L in regulation and control of seed size, and belongs to the technical field of plant genetic engineering. According to the invention, an MdMYB41L gene is cloned from apple GL-3, the CDS nucleotide sequence of the MdMYB41L gene is shown as SEQ ID NO: 1, and a plant overexpression vector is constructed. A transgenic plant is obtained by heterologous transformation into arabidopsis thaliana through an agrobacterium tumefaciens-mediated method. Compared with wild arabidopsis thaliana, the weight, the length, the width and the surface area of the transgenic plant seeds are obviously improved. In addition, the cotyledon area of a transgenic plant is obviously higher than that of a wild type. Therefore, the MYB transcription factor MdMYB41L provides a new thought in the aspects of improving the plant seed size and molecular breeding.

To provide methods and compositions for controlling sucker growth in tobacco by altering the expression of different target genes.SOLUTION: A modified tobacco plant comprises a mutation in an endogenous gene encoding polypeptide selected from the group consisting of cyclin, cyclin-dependent kinase (CDK), a CDK inhibitor, MYB, and a WRKY transcription factor, wherein the mutation is not present in the endogenous gene in a control tobacco plant of the same variety, and wherein the modified tobacco plant comprises no suckers or reduced suckers after topping as compared to the control tobacco plant when grown under comparable growth conditions.SELECTED DRAWING: None

The invention relates to the technical field of gene engineering, and provides a lycium ruthenicum MYB transcription factor LrMYB77, the amino acid sequence of which is as shown in SEQ ID NO.3. The invention also provides a gene LrMYB77 for coding a transcription factor, and a recombinant expression vector and host bacteria containing the gene. Meanwhile, the invention further provides application of the transcription factor or the gene in promoting synthesis of lycium ruthenicum anthocyanin and a specific method for increasing the synthesis amount of the lycium ruthenicum anthocyanin. According to the invention, the LrMYB77 gene with a regulation effect on anthocyanin synthesis is screened from lycium ruthenicum for the first time, and experiments verify that the expression or overexpression of the gene in a plant is regulated, so that the synthesis and accumulation of anthocyanin in the plant can be remarkably promoted, and the LrMYB77 gene has important significance on germplasm resource creation and molecular breeding of lycium ruthenicum rich in anthocyanin.

The application belongs to the field of plant biotechnology, and particularly relates to a cucumber CsODO1 gene and application thereof, and is a MYB transcription factor capable of being combined with a CsCSE1 promoter, which is screened through a yeast single hybridization. It is proved through EMSA and tobacco transient expression analysis that the CsODO1 gene directly and specifically combines with a "CAACCA" sequence in the CsCSE1 promoter, and inhibits the expression of the CsCSE1 gene. The CsODO1 gene can be induced to express by a powdery mildew fungus, and is differentially expressed in the cucumber infected by the powdery mildew fungus. It is found through a cucumber cotyledon transient transformation system mediated by an agrobacterium that the CsODO1 gene negatively regulates the biosynthesis of lignin and the resistance of the cucumber to the powdery mildew fungus. The application provides a new reference gene resource for the cultivation of a cucumber disease-resistant variety, and provides a new thought for the research on the molecular mechanism of the plant immunity mediated by the lignin pathway.

This invention discloses a gene, AcMYB22, that regulates the biosynthesis of flavonoids in kiwifruit and its applications, belonging to the field of genetic engineering technology. This invention clones two alleles of the AcMYB22 transcription factor, AcMYB22-1 and AcMYB22-2, from the 'Hongyang' kiwifruit and its bud mutations. Plant expression vectors of AcMYB22-1 and AcMYB22-2 were constructed, and overexpressed kiwifruit fruits and stably overexpressed kiwifruit plants were obtained using Agrobacterium-mediated transformation. The overexpressed materials showed a promotion of flavonoid and anthocyanin accumulation. This research enriches the mechanism by which MYB transcription factors regulate the biosynthesis of flavonoids in kiwifruit, provides technical support for improving plant flavonoid synthesis, and is of great significance for the quality improvement and new variety breeding of kiwifruit.

The invention discloses a molecular marker of a wheat MYB transcription factor family member TaRVE1-6B and application of the molecular marker, and belongs to the technical field of crop seed selection and cultivation. The molecular marker comprises RVE1-6B-527566461, RVE1-6B-527565157 and RVE1-6B-527561616, the genotypes of the InDel2 site, the InDel5 site and the InDel8 site can be determined according to the size of an amplification band of the molecular marker, and the haplotype of TaRVE1-6B can be determined according to the genotypes of the InDel2 site, the InDel5 site and the InDel8 site. The molecular marker has the beneficial effects that an excellent allele of TaRVE1-6B is explored from a natural population of wheat, and the molecular marker for identifying the plant height, the ear length and the thousand seed weight of wheat is developed, so that a gene resource and an effective way are provided for genetic improvement of wheat yield traits.

Disclosed are use of the Camellia sinensis (L.) O. Kuntz CsMYB1 gene in improving disease resistance of a plant and a method for improving disease resistance of a plant. The CsMYB1 gene is a transcription factor gene of the MYB family. CsMYB1 is constructed into a plant expression vector, and the vector is transferred into Nicotiana benthamiana. A transgenic tobacco plant is obtained by means of stable genetic transformation, and the plant exhibits relatively strong fungal inhibitory activity against inoculated Botrytis cinerea hyphae. Transient silencing of the CsMYB1 gene of Camellia sinensis (L.) O. Kuntz leaves using a technology of antisense oligonucleotides (AsODNs) significantly reduces the disease resistance of the plant. Therefore, the CsMYB1 gene has the effect of improving the disease resistance of a plant and can be used as a disease-resistant gene. Introducing the gene into plants such as tobacco, Camellia sinensis (L.) O. Kuntz, or vegetables can improve the disease resistance of these plants. The present invention has broad market application prospects.

The application discloses a gene related to osmanthus fragrans carotenoid synthesis OfPHL5 and application thereof OfPHL5 The gene is a MYB transcription factor gene, and an overexpression vector containing the gene OfPHL5 is transformed into osmanthus fragrans petals to perform transient expression. OfPHL5 The results show that the gene expression amount of the transiently transformed osmanthus fragrans petals is significantly increased, and the total carotenoid content in the petals is increased, which indicates that the gene plays an important role in promoting osmanthus fragrans carotenoid synthesis. OfPHL5 The gene can be used for improving the color quality of osmanthus fragrans and excellent variety breeding in osmanthus fragrans genetic engineering.

The invention relates to application of a coding gene of a corn MYB transcription factor ZmMYBIF35 in regulation and control of plant drought tolerance, and belongs to the technical field of gene engineering and crop genetic breeding, the CDS sequence of the coding gene of the transcription factor ZmMYBIF35 is as shown in SEQ ID NO.1, the genome sequence is as shown in SEQ ID NO.2, the gene is used for positively regulating and controlling the drought tolerance of crop plants, and the amino acid sequence of the transcription factor ZmMYBIF35 is as shown in SEQ ID NO.3. The invention also relates to application of the coding gene of the corn MYB transcription factor ZmMYBIF35 in regulation and control of plant drought tolerance. The ZmMYBIF35 gene overexpression corn plant is analyzed, it is found that the ZmMYBIF35 gene can regulate and control the drought tolerance of the plant, ZmMYBIF35 gene overexpression can enhance the drought tolerance of the plant, and important theoretical significance and application value are provided for creation of new corn germplasm and corn molecular breeding.

The invention discloses an HAPP gene and application thereof, and relates to the field of plant genetic engineering, the identified rice HAPP gene can increase the rice plant height and the seed size at the same time, and after the rice HAPP gene is fused with a PANDA gene, the rice plant height and the seed size can be remarkably increased, but the ear number is reduced. Meanwhile, analysis finds that HAPP originates from a Harbinger transposon Myb-like DNA binding protein, interacts with PANDA and is related to a gene expression mode, but has antagonism in function. Therefore, identification of the HAPP gene not only reveals a new function of the transposon-derived gene in regulation and control of rice yield traits, but also provides new theoretical support and practical guidance for rice breeding.

This invention discloses a wheat blue-grain gene and its applications, belonging to the fields of plant molecular biology, biochemistry, genetics, and plant breeding. Using differential expression analysis between blue-grain and white-grain wheat, four genes controlling the blue-grain trait were identified: two MYB family transcription factors and two bHLH family transcription factors. Furthermore, plant recombinant expression vectors for these genes and a method for regulating anthocyanin synthesis in plants are provided. This has significant theoretical and practical implications for studying the aleurone layer pigment synthesis pathway in blue-grain wheat, its use as a screening marker in plant transformation processes, and improving the nutritional value of plants.

The invention relates to a primer and a probe for quantitatively detecting MYB-PLAGL1 and PLEKHG1-ABL1 fusion genes, a kit and a use method of the kit. The kit is characterized in that 1, the kit comprises an MYB-PLAGL1 primer, a PLEKHG1-ABL1 primer and an ABL1 internal reference primer; the nucleotide sequences of the MYB-PLAGL1 primer comprise SEQ ID NO: 1 and SEQ ID NO: 2, and the nucleotide sequences of the PLEKHG1-ABL1 primer comprise SEQ ID NO: 3 and SEQ ID NO: 4. The invention finds and verifies that a T-ALL patient carries two new fusion genes of MYB-PLAGL1 and PLEKHG1-ABL1, and the fusion genes can be used as molecular markers of the T-ALL patient and are applied to disease diagnosis, treatment scheme selection and minimal residual focus monitoring.

The invention discloses a tea tree MYB transcription factor CsMYB330 gene and application, the nucleotide sequence of the MYB transcription factor CsMYB330 gene is shown as SEQ ID NO: 1, the expression of the gene participates in regulating the flavonol content in plants, and the instantaneous silencing of the CsMYB330 gene in the tea tree can significantly reduce the flavonol content in the tea tree by 24.5% and reduce the bitter taste of the finished tea.

The invention relates to the technical field of plant molecular biology, in particular to application of OsMYB52 protein or a coding gene of the OsMYB52 protein or a biological material containing the coding gene of the OsMYB52 protein to regulation and control of the heading stage of plants. The invention discloses an MYB transcription factor OsMYB52 for negative regulation and control of the heading stage of rice. The gene interacts with Hd1 to enhance the transcriptional activation effect of Hd1 on Ghd7 and Ghd7.1 and inhibit the transcriptional activation of Hd1 on Ehd1 and Hd3a, so that the heading stage of rice is negatively regulated, and the rice yield is changed. The function and the regulation mechanism of the OsMYB52 for regulating the heading stage of the rice are reported for the first time, and the OsMYB52 has important guiding significance for perfecting the molecular mechanism of a rice flowering regulation network and improving the yield and the quality of the rice.

The present application relates to the technical field of plant molecular biology, and particularly relates to application of OsMYB52 protein, or a coding gene thereof, or a biological material containing the coding gene of the OsMYB52 protein in regulating a heading stage of a plant. The present application discloses a MYB transcription factor for negatively regulating a heading stage of rice OsMYB52 . The gene enhances transcription activation of Hd1 on Ghd7 , Ghd7.1 by interacting with Hd1, inhibits transcription activation of Hd1 on Ehd1 , Hd3a , and further negatively regulates the heading stage of rice and causes change of yield of rice. OsMYB52 The function and regulation mechanism of regulating the heading stage of rice are reported for the first time, and the function and regulation mechanism have important guiding significance for perfecting a molecular mechanism of a flowering regulation network of rice and improving yield and quality of rice.

An induction method for an Antirrhinum majus L. hairy root producing an anthocyanin and a glycoside derivative thereof, a method for screening an inducing gene, and production and use, specifically belonging to the technical field of plant tissue culturing. The induction method for an Antirrhinum majus L. hairy root producing an anthocyanin and a glycoside derivative thereof comprises the following steps: using Agrobacterium rhizogenes containing an inducing gene to infect an Antirrhinum majus L. explant, co-culturing the Agrobacterium rhizogenes and the Antirrhinum majus L. explant, and performing sterilization culture to obtain the Antirrhinum majus L. hairy root producing the anthocyanin and the glycoside derivative thereof. The inducing gene comprises a gene encoding an MYB transcription factor, or a mutant thereof. The induction method can quickly and effectively obtain the hairy root synthesizing the anthocyanin and the glycoside derivative thereof while realizing the acquisition of the anthocyanin and the glycoside derivative thereof in a short time without being affected by seasons in the case of limited germplasm resources.

The application discloses an MYB transcription factor for changing the color of Paphiopedilum callosum and application of the MYB transcription factor. The nucleotide sequence of the transcription factor PpMYB1 is shown as SEQ ID NO. 1. The PpMYB1 gene is cloned from the petal of Paphiopedilum callosum, and is stably transformed into tobacco through an agrobacterium-mediated leaf disc method. The results show that overexpression of the PpMYB1 gene in tobacco makes the color of tobacco flowers deepen. The PpMYB1 gene is transformed into the petal of Paphiopedilum callosum through an agrobacterium-mediated transient expression technology. The results show that overexpression of the PpMYB1 gene in the petal of Paphiopedilum callosum makes the white petal present purplish red. It is proved that the PpMYB1 gene of Paphiopedilum callosum has the function of regulating the synthesis of anthocyanin of flowers.

This invention discloses the application of the cotton GhMYB transcription factor GhMYB4 gene in increasing cotton fiber length. This invention utilizes biotechnology to clarify that the GhMYB4 gene has a negative regulatory effect on fiber cell elongation, and downregulation of GhMYB4 gene expression leads to a significant increase in fiber length. Studies have shown that auxin content and auxin response pathways were significantly altered in GhMYB4-silenced cotton lines and transgenic Arabidopsis. In cotton fibers from GhMYB4-silenced lines after 20 days of development, Cers content was significantly increased. GhMYB4 inhibits the expression of the lipid transporter gene GhLTP4 by binding to the MYB cis-element on the GhLTP4 promoter. GhMYB4 affects fiber cell development by regulating auxin content, auxin response pathways, and lipid content. Regulating fiber cell elongation by inhibiting GhMYB4 expression has high breeding application value.

Single chain peptides comprising either a cell penetrating HIV-TAT peptide sequence and a MYB:CBP complex interfering peptide sequence from MYB, or comprising a cell penetrating HIV-TAT peptide sequence, a CBP binding peptide sequence from CREB and a MYB:CBP complex interfering peptide sequence from MYB, are provided for use in preventing MYB:CBP complex formation and downstream events leading to cancer, in particular a leukemia. Both L-amino acid single chain peptides and retro-inverso single chain peptides are provided.

The invention discloses a cinnamomum camphora MYB transcription factor gene, and belongs to the technical field of forest metabolism regulation. The nucleotide sequence of the gene is as shown in SEQ ID NO.5. The amino acid sequence of the transcription factor is as shown in SEQ ID No.7. The gene provided by the invention is one of key genes in a cinnamomum camphora metabolism process, and can regulate and control flavonoid metabolism, terpenoid metabolism, organic acid metabolism and the like of cinnamomum camphora. The gene provides an important theoretical basis for the industrial production in the fields of cinnamomum camphora breeding, medicines, spices and the like in the future, and has a wide application prospect and a great economic value.

The application discloses a tea tree MYB transcription factor gene CsMYB4 and application thereof, and belongs to the technical field of biotechnology.The CDS sequence of the gene is shown as SEQ ID NO:1, and the gene encodes a MYB transcription factor.The CsMYB4 gene has the function of improving the fungal resistance of plants, the fungal resistance gene CsMYB4 is constructed on a plant expression vector and is introduced into Nicotiana benthamiana to perform transient overexpression, or is stably genetically transformed into tobacco plants, and all have strong in-vitro fungal resistance activity, the transgenic tobacco with overexpression of CsMYB4 has strong bacteriostatic activity on Botrytis cinerea and Epicoccum sorghinum, after the CsMYB4 gene is silenced by using a virus induced gene silencing (VIGS) technology, the growth of the plants is hindered, and the disease resistance is greatly weakened.

The invention discloses a dendrobium MYB transcription factor gene DoMYB59 and application of the dendrobium MYB transcription factor gene DoMYB59. The invention relates to application of a transcription factor DoMYB59 or a coding gene DoMYB59 thereof in regulation and control of synthesis of rutin in plants. The amino acid sequence of the transcription factor DoMYB59 is shown as SEQ ID NO.2. The invention also relates to application of the transcription factor DoMYB59 in regulation and control of synthesis of rutin in plants. A coding gene MYB transcription factor DoMYB59 belonging to a dendrobium MYB transcription factor DoMYB59 is cloned from dendrobium officinale leaves, the MYB transcription factor DoMYB59 is used as a positive regulation factor to participate in regulation of a rutin synthesis process of dendrobium, and transient expression and functional identification prove that the rutin content of transgenic dendrobium is increased when the MYB transcription factor DoMYB59 is excessively expressed. Therefore, the MYB transcription factor DoMYB59 or the coding gene thereof has important theoretical guidance significance and application value in the fields of secondary metabolite synthesis and medical application of orchid plants.

The application discloses a gene BpMIXTA01 for regulating and controlling initial development of Broussonetia papyrifera epidermal hair as well as an expression protein and application thereof, and belongs to the technical field of plant genetic engineering. The nucleotide sequence of the gene is shown in SEQ ID NO. 1, and the amino acid sequence is shown in SEQ ID NO. 2. The gene belongs to the ninth sub-group MIXTA gene of the R2R3 MYB transcription factor family. Function verification shows that overexpression of BpMIXTA01 in Arabidopsis thaliana can significantly increase the number of epidermal hairs, and can restore the epidermal hair loss phenotype of the Arabidopsis thaliana hairless mutant, proving that BpMIXTA01 is a positive regulation factor of epidermal hair formation. The application first discloses a key regulation gene for initial development of Broussonetia papyrifera epidermal hair cells, provides an important gene resource for analyzing the molecular mechanism of epidermal hair development of woody plants, and can cultivate a hairless forage Broussonetia papyrifera new variety through genetic engineering means, and has important theoretical value and application prospect.