52 results about "MYB" patented technology

The invention discloses an application of a gene OsRNM5 with a Myb structural domain. The cDNA (complementary deoxyribonucleic acid) sequence of the OsRNM5 is SEQ ID NO.1, and the amino acid sequence coded by the OsRNM5 is SEQ ID NO.2. Field yield measurement shows that compared with a wild type WYJ7, the plant height of an OsRNM5 overexpression material is reduced, the yield of a single plant is reduced, and the plant height of an OsRNM5 knockout material is increased, and the yield of the single plant is increased. By knocking out or silencing the OsRNM5 gene in the rice, the nitrogen fertilizer utilization efficiency and yield of the rice can be improved.

The invention relates to the technical field of genetic engineering and molecular breeding, in particular to a ramie MYB transcription factor and application thereof. The MYB transcription factor is BnMYB6, the amino acid sequence of the MYB transcription factor is as shown in SEQ ID NO.1, and the gene sequence for coding the amino acid of the MYB transcription factor is as shown in SEQ ID NO.2. The transcription factor protein BnMYB6 can specifically and negatively regulate the biosynthesis of plant lignin, and has application value in the aspects of inhibiting the synthesis of plant lignin, promoting the growth and development of a root system and maintaining related traits such as the activity of the root system under drought stress.

The invention discloses application of apple MdMYB41L in regulation and control of seed size, and belongs to the technical field of plant genetic engineering. According to the invention, an MdMYB41L gene is cloned from apple GL-3, the CDS nucleotide sequence of the MdMYB41L gene is shown as SEQ ID NO: 1, and a plant overexpression vector is constructed. A transgenic plant is obtained by heterologous transformation into arabidopsis thaliana through an agrobacterium tumefaciens-mediated method. Compared with wild arabidopsis thaliana, the weight, the length, the width and the surface area of the transgenic plant seeds are obviously improved. In addition, the cotyledon area of a transgenic plant is obviously higher than that of a wild type. Therefore, the MYB transcription factor MdMYB41L provides a new thought in the aspects of improving the plant seed size and molecular breeding.

The invention relates to the technical field of gene engineering, and provides a lycium ruthenicum MYB transcription factor LrMYB77, the amino acid sequence of which is as shown in SEQ ID NO.3. The invention also provides a gene LrMYB77 for coding a transcription factor, and a recombinant expression vector and host bacteria containing the gene. Meanwhile, the invention further provides application of the transcription factor or the gene in promoting synthesis of lycium ruthenicum anthocyanin and a specific method for increasing the synthesis amount of the lycium ruthenicum anthocyanin. According to the invention, the LrMYB77 gene with a regulation effect on anthocyanin synthesis is screened from lycium ruthenicum for the first time, and experiments verify that the expression or overexpression of the gene in a plant is regulated, so that the synthesis and accumulation of anthocyanin in the plant can be remarkably promoted, and the LrMYB77 gene has important significance on germplasm resource creation and molecular breeding of lycium ruthenicum rich in anthocyanin.

The application belongs to the field of plant biotechnology, and particularly relates to a cucumber CsODO1 gene and application thereof, and is a MYB transcription factor capable of being combined with a CsCSE1 promoter, which is screened through a yeast single hybridization. It is proved through EMSA and tobacco transient expression analysis that the CsODO1 gene directly and specifically combines with a "CAACCA" sequence in the CsCSE1 promoter, and inhibits the expression of the CsCSE1 gene. The CsODO1 gene can be induced to express by a powdery mildew fungus, and is differentially expressed in the cucumber infected by the powdery mildew fungus. It is found through a cucumber cotyledon transient transformation system mediated by an agrobacterium that the CsODO1 gene negatively regulates the biosynthesis of lignin and the resistance of the cucumber to the powdery mildew fungus. The application provides a new reference gene resource for the cultivation of a cucumber disease-resistant variety, and provides a new thought for the research on the molecular mechanism of the plant immunity mediated by the lignin pathway.

The invention discloses a molecular marker of a wheat MYB transcription factor family member TaRVE1-6B and application of the molecular marker, and belongs to the technical field of crop seed selection and cultivation. The molecular marker comprises RVE1-6B-527566461, RVE1-6B-527565157 and RVE1-6B-527561616, the genotypes of the InDel2 site, the InDel5 site and the InDel8 site can be determined according to the size of an amplification band of the molecular marker, and the haplotype of TaRVE1-6B can be determined according to the genotypes of the InDel2 site, the InDel5 site and the InDel8 site. The molecular marker has the beneficial effects that an excellent allele of TaRVE1-6B is explored from a natural population of wheat, and the molecular marker for identifying the plant height, the ear length and the thousand seed weight of wheat is developed, so that a gene resource and an effective way are provided for genetic improvement of wheat yield traits.

Disclosed are use of the Camellia sinensis (L.) O. Kuntz CsMYB1 gene in improving disease resistance of a plant and a method for improving disease resistance of a plant. The CsMYB1 gene is a transcription factor gene of the MYB family. CsMYB1 is constructed into a plant expression vector, and the vector is transferred into Nicotiana benthamiana. A transgenic tobacco plant is obtained by means of stable genetic transformation, and the plant exhibits relatively strong fungal inhibitory activity against inoculated Botrytis cinerea hyphae. Transient silencing of the CsMYB1 gene of Camellia sinensis (L.) O. Kuntz leaves using a technology of antisense oligonucleotides (AsODNs) significantly reduces the disease resistance of the plant. Therefore, the CsMYB1 gene has the effect of improving the disease resistance of a plant and can be used as a disease-resistant gene. Introducing the gene into plants such as tobacco, Camellia sinensis (L.) O. Kuntz, or vegetables can improve the disease resistance of these plants. The present invention has broad market application prospects.

The invention discloses an HAPP gene and application thereof, and relates to the field of plant genetic engineering, the identified rice HAPP gene can increase the rice plant height and the seed size at the same time, and after the rice HAPP gene is fused with a PANDA gene, the rice plant height and the seed size can be remarkably increased, but the ear number is reduced. Meanwhile, analysis finds that HAPP originates from a Harbinger transposon Myb-like DNA binding protein, interacts with PANDA and is related to a gene expression mode, but has antagonism in function. Therefore, identification of the HAPP gene not only reveals a new function of the transposon-derived gene in regulation and control of rice yield traits, but also provides new theoretical support and practical guidance for rice breeding.

The invention discloses a tea tree MYB transcription factor CsMYB330 gene and application, the nucleotide sequence of the MYB transcription factor CsMYB330 gene is shown as SEQ ID NO: 1, the expression of the gene participates in regulating the flavonol content in plants, and the instantaneous silencing of the CsMYB330 gene in the tea tree can significantly reduce the flavonol content in the tea tree by 24.5% and reduce the bitter taste of the finished tea.

An induction method for an Antirrhinum majus L. hairy root producing an anthocyanin and a glycoside derivative thereof, a method for screening an inducing gene, and production and use, specifically belonging to the technical field of plant tissue culturing. The induction method for an Antirrhinum majus L. hairy root producing an anthocyanin and a glycoside derivative thereof comprises the following steps: using Agrobacterium rhizogenes containing an inducing gene to infect an Antirrhinum majus L. explant, co-culturing the Agrobacterium rhizogenes and the Antirrhinum majus L. explant, and performing sterilization culture to obtain the Antirrhinum majus L. hairy root producing the anthocyanin and the glycoside derivative thereof. The inducing gene comprises a gene encoding an MYB transcription factor, or a mutant thereof. The induction method can quickly and effectively obtain the hairy root synthesizing the anthocyanin and the glycoside derivative thereof while realizing the acquisition of the anthocyanin and the glycoside derivative thereof in a short time without being affected by seasons in the case of limited germplasm resources.

The application discloses an MYB transcription factor for changing the color of Paphiopedilum callosum and application of the MYB transcription factor. The nucleotide sequence of the transcription factor PpMYB1 is shown as SEQ ID NO. 1. The PpMYB1 gene is cloned from the petal of Paphiopedilum callosum, and is stably transformed into tobacco through an agrobacterium-mediated leaf disc method. The results show that overexpression of the PpMYB1 gene in tobacco makes the color of tobacco flowers deepen. The PpMYB1 gene is transformed into the petal of Paphiopedilum callosum through an agrobacterium-mediated transient expression technology. The results show that overexpression of the PpMYB1 gene in the petal of Paphiopedilum callosum makes the white petal present purplish red. It is proved that the PpMYB1 gene of Paphiopedilum callosum has the function of regulating the synthesis of anthocyanin of flowers.

This invention discloses the application of the cotton GhMYB transcription factor GhMYB4 gene in increasing cotton fiber length. This invention utilizes biotechnology to clarify that the GhMYB4 gene has a negative regulatory effect on fiber cell elongation, and downregulation of GhMYB4 gene expression leads to a significant increase in fiber length. Studies have shown that auxin content and auxin response pathways were significantly altered in GhMYB4-silenced cotton lines and transgenic Arabidopsis. In cotton fibers from GhMYB4-silenced lines after 20 days of development, Cers content was significantly increased. GhMYB4 inhibits the expression of the lipid transporter gene GhLTP4 by binding to the MYB cis-element on the GhLTP4 promoter. GhMYB4 affects fiber cell development by regulating auxin content, auxin response pathways, and lipid content. Regulating fiber cell elongation by inhibiting GhMYB4 expression has high breeding application value.

Single chain peptides comprising either a cell penetrating HIV-TAT peptide sequence and a MYB:CBP complex interfering peptide sequence from MYB, or comprising a cell penetrating HIV-TAT peptide sequence, a CBP binding peptide sequence from CREB and a MYB:CBP complex interfering peptide sequence from MYB, are provided for use in preventing MYB:CBP complex formation and downstream events leading to cancer, in particular a leukemia. Both L-amino acid single chain peptides and retro-inverso single chain peptides are provided.

The invention discloses a dendrobium MYB transcription factor gene DoMYB59 and application of the dendrobium MYB transcription factor gene DoMYB59. The invention relates to application of a transcription factor DoMYB59 or a coding gene DoMYB59 thereof in regulation and control of synthesis of rutin in plants. The amino acid sequence of the transcription factor DoMYB59 is shown as SEQ ID NO.2. The invention also relates to application of the transcription factor DoMYB59 in regulation and control of synthesis of rutin in plants. A coding gene MYB transcription factor DoMYB59 belonging to a dendrobium MYB transcription factor DoMYB59 is cloned from dendrobium officinale leaves, the MYB transcription factor DoMYB59 is used as a positive regulation factor to participate in regulation of a rutin synthesis process of dendrobium, and transient expression and functional identification prove that the rutin content of transgenic dendrobium is increased when the MYB transcription factor DoMYB59 is excessively expressed. Therefore, the MYB transcription factor DoMYB59 or the coding gene thereof has important theoretical guidance significance and application value in the fields of secondary metabolite synthesis and medical application of orchid plants.

The application discloses a gene BpMIXTA01 for regulating and controlling initial development of Broussonetia papyrifera epidermal hair as well as an expression protein and application thereof, and belongs to the technical field of plant genetic engineering. The nucleotide sequence of the gene is shown in SEQ ID NO. 1, and the amino acid sequence is shown in SEQ ID NO. 2. The gene belongs to the ninth sub-group MIXTA gene of the R2R3 MYB transcription factor family. Function verification shows that overexpression of BpMIXTA01 in Arabidopsis thaliana can significantly increase the number of epidermal hairs, and can restore the epidermal hair loss phenotype of the Arabidopsis thaliana hairless mutant, proving that BpMIXTA01 is a positive regulation factor of epidermal hair formation. The application first discloses a key regulation gene for initial development of Broussonetia papyrifera epidermal hair cells, provides an important gene resource for analyzing the molecular mechanism of epidermal hair development of woody plants, and can cultivate a hairless forage Broussonetia papyrifera new variety through genetic engineering means, and has important theoretical value and application prospect.

The invention provides a co-expression vector and a method for changing the type of biosynthetic anthocyanin glycoside, and belongs to the technical field of genetic engineering. The invention provides a co-expression vector containing a gene overexpression box and a gene knockout box, and an MYB transcription factor overexpression box and an anthocyanin rhamnoside transferase gene knockout box are jointly constructed on a basic skeleton vector. Therefore, the co-expression vector can simultaneously achieve the effects of overexpressing MYB and knocking out the anthocyanin rhamnoside transferase gene. In the embodiment of the invention, by taking antirhubarb as an example, genetic transformation is completed by utilizing the co-expression vector, and gene knockout and overexpression are carried out through one vector, so that the conversion of anthocyanin from rhamnoside to glucoside is realized, for example, the conversion of cyanidin rhamnoside to cyanidin glucoside is realized, and the conversion of anthocyanin from rhamnoside to cyanidin glucoside is realized. Therefore, the content of glucoside in the anthocyanin glycoside is obviously improved.

The invention discloses a transcription factor gene IbMYB11 for regulating and controlling synthesis of sweet potato chlorogenic acid and application of the transcription factor gene IbMYB11, and belongs to the field of plant genetic engineering. Aiming at weak links of sweet potato chlorogenic acid synthesis regulation and control, the transcription factor IbMYB11 gene related to sweet potato chlorogenic acid synthesis regulation and control is cloned for the first time. The IbMYB11 belongs to an S7 subfamily, is positioned in a cell nucleus and has transcriptional activation activity, and accumulation of chlorogenic acid can be remarkably promoted by overexpressing the IbMYB11 gene in sweet potatoes. The sweet potato MYB transcription factor gene IbMYB11 provided by the invention can be used as an excellent gene resource, is widely applied to the field of plant genetic breeding, and has important significance for improving metabolism accumulation of chlorogenic acid substances of plants, especially for cultivating plant varieties with high chlorogenic acid content.

The invention relates to the technical field of plant genetic engineering, in particular to application of a transcription factor MYB46 to regulation and control of temperature response growth of mesocotyl of a plant. It is found that the plant MYB family transcription factor MYB46 is a negative regulation factor responding to the rice mesocotyl at the high environment temperature, the mesocotyl length of the MYB46 knockout mutant is remarkably longer than that of a wild type at the high environment temperature, the MYB46 knockout mutant is more sensitive to the high environment temperature response, and the MYB46 has important application value in direct seeding production of rice at the high temperature in summer. The discovery of the new function of the MYB46 provides new gene resources and methods for cultivating rice varieties suitable for direct seeding.

This invention discloses a Panax notoginseng MYB transcription factor gene. PnMYB34 Its nucleotide sequence is as described in SEQ ID NO:1, encoding the MYB transcription factor. This invention confirms through molecular biology and functional genomics related technologies. PnMYB34 The gene has the ability to enhance the plant's resistance to viral infection, and the present invention will... PnMYB34 Genes were constructed into plant expression vectors and overexpressed in Panax notoginseng and tobacco, respectively. Experimental results showed that overexpression... PnMYB34 Genetically modified Panax notoginseng against Panax notoginseng A virus (GMO) Panax notoginseng virus A The resistance to ) was significantly enhanced, and overexpression PnMYB34 Genetically modified tobacco is susceptible to tobacco mosaic virus (BMP). Tobacco mosaic virus Its resistance was also significantly enhanced.

Pharmaceutical compositions and compounds comprising a phosphorothioated CpG oligodeoxynucleotide linked to a DNA oligonucleotide that is hybridized an RNA aptamer are useful in methods of treating cancer (such as leukemia) and methods of inhibiting DNA methyltransferase. In embodiments, the RNA aptamer binds to an intracellular target such as DNMT1, NF-kB, RUNX1, MYC, MYB, ETS, PAX5, MDM2, F0XM1, PU.l, STAT3, STATS. STAT6, FAD, ATP5B, or beta-catenin.

This invention discloses a regulatory gene BpMYB090 for the development of epidermal hairs on paper mulberry leaves, its expressed protein, and its applications, belonging to the field of plant genetic engineering technology. The nucleotide sequence of this gene, BpMYB090, is shown in SEQ ID NO.1. This gene belongs to the ninth subgroup of the R2R3 MYB transcription factor family, MIXTA class, and its expression level is significantly positively correlated with the density of epidermal hairs on paper mulberry leaves. Overexpression of BpMYB090 in Arabidopsis thaliana significantly increases the number of epidermal hairs and restores the epidermal hair-deficient phenotype of the Arabidopsis thaliana mutant gl1, confirming that BpMYB090 is a positive regulator of epidermal hair formation. This invention reveals for the first time a key regulatory gene for the development of epidermal hairs on paper mulberry leaves. Furthermore, based on the positive regulatory function of this gene, new hairless forage paper mulberry varieties can be bred through gene silencing or editing techniques, possessing significant theoretical value and application prospects.

The invention relates to the technical field of plant genetic engineering, and discloses an azalea MYB transcription factor RhMYB8 and application thereof. The nucleotide sequence of the rhododendron MYB transcription factor RhMYB8 is as shown in SEQ ID NO. 1, and the amino acid sequence coded by the rhododendron MYB transcription factor RhMYB8 is as shown in SEQ ID NO. 2. The rhododendron MYB transcription factor RhMYB8 is over-expressed in the rhododendron, so that the cold resistance of the rhododendron is improved. By adopting the rhododendron MYB transcription factor RhMYB8 and the application thereof, a new functional gene and a core target are provided for horticultural plant cold-resistant molecular breeding, and theoretical and technical foundations are laid for improving the low-temperature stress resistance of plants and cultivating new varieties of cold-resistant plants by using molecular means; the method has an important application value in quality and efficiency improvement and cold-resistant variety breeding in the azalea industry.

The invention discloses a cyhalofop-butyl-resistant digitaria sanguinalis MYB transcription factor gene and application thereof, and relates to the field of plant genetic engineering, and the expression of the gene in a resistant digitaria sanguinalis biotype is remarkably up-regulated. After a plant expression vector is constructed and corn is transformed, after 11.25 g a.i. Ha-1 cyhalofop-butyl is sprayed on a transgenic corn plant in a 3-4 leaf period, the pesticide resistance and the survival rate are obviously enhanced, and the transgenic corn plant is obviously superior to a wild type. The invention proves that the MYB gene can endow plants with cyhalofop-butyl resistance, provides a new gene resource for cultivating transgenic crops resisting acetyl-CoA carboxylase (ACCase) inhibitor herbicides, and has important application value.

The invention discloses an R2R3-MYB transcription factor for specifically regulating and controlling anthocyanin accumulation in a spotted region of a dendrobium nobile flower and application of the R2R3-MYB transcription factor, the R2R3-MYB transcription factor comprises an R2R3-MYB gene-DnMYB1, and the application of the R2R3-MYB transcription factor comprises the following steps: carrying out targeted regulation and control through a transgenic technology, and carrying out transgenic breeding; in the phalaenopsis and white flower gardening variety, the heterologous transient overexpression of the target gene can significantly promote the accumulation of anthocyanin in petals of the phalaenopsis and white flower gardening variety; in the wild type dendrobium nobile, the accumulation of the anthocyanin in the floral quilt of the wild type dendrobium nobile can be obviously reduced through targeted silencing of the target gene. The gene plays an important role in the aspect of anthocyanin biosynthesis, can enrich a transgenic material library in which specific pigments are accumulated in a spot region of the orchid, and provides powerful support for molecular breeding for cultivating a diversity coloring mode of the orchid in the future.

The application discloses an alfalfa MYB transcription factor MsMYB306 gene and application thereof, the nucleotide sequence of the MYB transcription factor MsMYB306 gene is as follows (1) or (2): (1) the nucleotide sequence shown in SEQ ID NO. 1; (2) a nucleotide sequence with more than 90% homology with the nucleotide sequence and equivalent functions of (1). The application provides a method for cultivating cold-resistant plants by using the MsMYB306 gene, the cold resistance of transgenic alfalfa is improved after the gene is interfered with expression in alfalfa; meanwhile, the cold resistance of transgenic alfalfa is reduced after the gene is overexpressed in alfalfa.

The invention discloses an MYB transcription factor LrMYB12 related to synthesis of a lycium ruthenicum flavonoid substance and application of the MYB transcription factor LrMYB12. Belongs to the technical field of gene engineering. According to the invention, the LrMYB12 transcription factor closely related to flavonoid synthesis and the coding gene thereof are cloned and identified from lycium ruthenicum for the first time, a new key component is supplemented for analyzing a transcription regulation network for lycium ruthenicum flavonoid biosynthesis, and the blank in the prior art is filled. The LrMYB12 gene disclosed by the invention can be used as an important functional gene, is used for creating a new lycium barbarum germplasm with significantly increased flavonoid content through modern molecular breeding technologies such as transgenosis, gene editing or molecular marker-assisted selection, and has clear application prospects and industrial values in the aspects of functional food development and medicinal material quality improvement.

The invention relates to the field of plant genetic engineering, in particular to application of an MYB transcription factor gene HvPHL2 in regulating and controlling the content of beta-glucan in barley grains. The MYB transcription factor gene HvPHL2 participating in regulation and control of the barley grain beta-glucan content is found, and through construction experiments of yeast single impurity, gel migration, dual luciferase and overexpression genetic materials, it is proved that the transcription factor HvPHL2 can be combined to CCAAT-box of a promoter region of a barley grain beta-glucan synthesis major gene HvCslf6, expression of an HvCslf6 promoter is inhibited, and the content of the barley grain beta-glucan in the barley grain beta-glucan synthesis major gene HvCslf6 in the barley grain beta-glucan synthesis major gene HvCslf6 in the barley grain beta-glucan synthesis major gene HvCslf6 is regulated and controlled. The method plays a key role in regulation and control of beta-glucan synthesis, and provides a new theoretical basis for genetic regulation and control of the beta-glucan content of the barley grains.

The application discloses a SACC-83 cell line stably expressing a MYB-NFIB fusion gene and a construction method and application thereof. The SACC-83 cell line comprises a MYB-NFIB fusion gene, and the MYB-NFIB fusion gene has a sequence as shown in SEQ ID NO. 1. The application provides a complete construction method for obtaining a target gene from a tissue sample, constructing a lentivirus recombinant plasmid and obtaining a cell line stably expressing the target gene, and the method is relatively fast, simple and low in cost. Meanwhile, the MYB-NFIB fusion gene overexpressed in the constructed stable tumor cell line has stronger proliferation, migration and invasion abilities and a stronger clone formation ability, the cell line is not contaminated by other cells, can be stably subcultured and can be used for mechanism research on ACC tumor occurrence, infiltration and metastasis.

This invention discloses a MYB transcriptional repressor, AsMYB054, related to the synthesis of 2-(2-phenylethyl) chromones in Aquilaria sinensis and its applications. Targeting the weak links in the synthesis and regulation of 2-(2-phenylethyl) chromone compounds, this invention clones for the first time the AsMYB054 gene, a transcriptional repressor related to the regulation of 2-(2-phenylethyl) chromone synthesis in Aquilaria sinensis. AsMYB054 is located in the cell nucleus and possesses transcriptional repressive activity, capable of binding to and inhibiting the activity of the promoters of enzyme genes AsPKS02 and AsPKS09, which are related to the biosynthesis of 2-(2-phenylethyl) chromone compounds. Inhibiting the expression of AsMYB054 using gene editing or RNA interference techniques can promote the accumulation of 2-(2-phenylethyl) chromone compounds in Aquilaria sinensis, showing broad application prospects and significant economic value.