104 results about "MYB" patented technology

The invention discloses a coumarin synthetic gene in black bamboo and application thereof, and belongs to the technical field of molecular biology. The invention provides transcription factors PnMYB4 and PnMYB4-1 for promoting synthesis of coumarin in black bamboos and application of the transcription factors PnMYB4 and PnMYB4-1. The PnMYB4 and the PnMYB4-1 belong to an MYB transcription factor family, the length of a complete coding frame of the PnMYB4 is 762 basic groups, and the coded protein contains 253 amino acids; the length of a complete coding frame of the PnMYB4-1 is 744 basic groups, and the coded protein contains 253 amino acids. The coumarin substances in arabidopsis thaliana, which are respectively overexpressed by PnMYB4 and PnMYB4-1, are obviously increased, and in addition, the expression levels of coumarin synthetic genes such as AtPAL1, At4CL1 and AtC4H in overexpressed plants are obviously increased.

The invention belongs to the technical field of gene engineering, and relates to a cyperus esculentus CeMYB154 gene capable of improving salt tolerance and active oxygen scavenging capacity and application of the cyperus esculentus CeMYB154 gene. The Cyperus esculentus CeMYB154 gene belongs to an MYB transcription factor family, the Cyperus esculentus CeMYB154 gene is screened and transformed into Arabidopsis thaliana through transcriptome sequencing, bioinformatics analysis, gene cloning, overexpression vector construction and recombinant strain overexpression, and analysis finds that under salt stress, compared with a wild type, the root length of a plant with the overexpressed CeMYB154 gene is obviously increased, the growth vigor is better, and the growth rate of the plant with the overexpressed CeMYB154 gene is higher. According to the present invention, the content of MDA and H2O2 is significantly reduced, and the activity of CAT, POD and SOD antioxidant enzymes is significantly enhanced, such that the overexpressed CeMYB154 gene can enhance the salt tolerance and the active oxygen scavenging ability of the plant, and the overexpressed CeMYB154 gene is proved to be the salt tolerance positive regulation factor; the CeMYB154 gene can be used in the field of salt-tolerant stress-resistant breeding of cyperus esculentus, and provides an important gene resource for salt-tolerant molecular breeding.

The invention discloses a corn ZmMYBR37 gene and an application of the corn ZmMYBR37 gene in regulating and controlling the size of corn kernels. The nucleotide sequence of the ZmMYBR37 gene is as shown in SEQ ID No: 1 in a sequence table. A mutant material with small grains and reduced hundred-grain weight is separated from a corn EMS mutant library, and a mutation site occurs on a function unknown gene ZmMYBR37, so that protein translation is terminated in advance. The information analysis finds that the gene coding protein has a conservative MYB structural domain, and the gene coding protein is speculated to belong to an MYB transcription factor family. Gene expression analysis finds that the gene is high in constitutive expression in corn and high in expression level in leaves and embryos. The corn material overexpressed with the ZmMYBR37 has the advantages that the grain size is increased, and the hundred-grain weight is obviously increased, so that the ZmMYBR37 has the potential of increasing the corn yield.

The invention discloses a panax notoginseng MYB transcription factor gene PnMYB8, the nucleotide sequence of the gene PnMYB8 is as shown in SEQ ID NO: 1, an MYB transcription factor is encoded, molecular biology and functional genomics related technologies prove that the PnMYB8 gene has the capability of improving fungal infection resistance of plants, the PnMYB8 gene is constructed on a plant expression vector and transferred into tobacco for overexpression, and the yield of the panax notoginseng MYB transcription factor gene PnMYB8 is improved. Experimental results show that the resistance of the transgenic tobacco overexpressed with the PnMYB8 to the tryptophan destructor (Cylindrocarpon destructor), the fusarium latericum (Fusarium latericum), the fusarium equiseti (Fusarium equiseti) and the phoma herbarum (Phoma herbarum) is obviously enhanced, and the resistance of the transgenic tobacco overexpressed with the PnMYB8 to the tryptophan destructor (Fusarium latericum) is obviously enhanced.

The invention is suitable for the technical field of gene engineering, and provides a soybean MYB transcription factor gene and application thereof in increasing the content of isoflavone. The soybean MYB transcription factor gene is GmMYB3a, and the nucleotide sequence of the soybean MYB transcription factor gene is as shown in SEQ ID NO. 1. According to the invention, a pTF101-GmMYB3a overexpression vector and an RNAi-GmMYB3a interference vector are successfully constructed, and the constructed vectors are transferred into a receptor soybean variety Williams 82. Experimental results show that the soybean strain with overexpression of the GmMYB3a gene shows higher isoflavone content, while the soybean strain with interference of the GmMYB3a gene shows lower isoflavone content, and the positive regulation effect of the GmMYB3a in the isoflavone biosynthesis process is proved. The invention not only provides an innovative molecular breeding strategy for cultivating soybean strains with high isoflavone content, but also has remarkable application potential and market prospect.

The present invention relates to a method for constructing a gene network through a single-cell transcriptome and a method for discovering key genes in differentiation using same, and a composition for the prevention, alleviation, or treatment of colon cancer using the target discovered through the method. The method for constructing a gene network of key genes according to the present invention employs single-cell transcriptome data and thus can be applied to all single-cell transcriptome data. The combination of MYB / HDAC2 / FOXA2 discovered upon application to colon cells can serve as a cancer treatment target that promotes the differentiation of colon cancer cells to revert same into differentiated normal cells.

The invention discloses application of a scutellaria baicalensis SbMYB8 gene in promotion of anthocyanin synthesis, the application comprises the steps that the SbMYB8 gene is constructed to a plant expression vector and introduced into plant cells to promote synthesis of plant anthocyanin, and the nucleotide sequence of the SbMYB8 gene is shown as SEQ ID NO: 1, or the amino acid sequence coded by the SbMYB8 gene is shown as SEQ ID NO: 2. The MYB transcription factor SbMYB8 gene with the function of promoting anthocyanin synthesis is cloned and identified from the scutellaria baicalensis for the first time, and the blank of a transcription regulation mechanism for regulating anthocyanin metabolism in the scutellaria baicalensis is filled; the SbMYB8 gene can significantly promote accumulation of anthocyanin in various plants, shows good heterologous expression stability and adaptability, and has wide cross-species application potential.

The invention discloses an application of a gene OsRNM5 with a Myb structural domain. The cDNA (complementary deoxyribonucleic acid) sequence of the OsRNM5 is SEQ ID NO.1, and the amino acid sequence coded by the OsRNM5 is SEQ ID NO.2. Field yield measurement shows that compared with a wild type WYJ7, the plant height of an OsRNM5 overexpression material is reduced, the yield of a single plant is reduced, and the plant height of an OsRNM5 knockout material is increased, and the yield of the single plant is increased. By knocking out or silencing the OsRNM5 gene in the rice, the nitrogen fertilizer utilization efficiency and yield of the rice can be improved.

The invention relates to the technical field of plant genetic engineering, and discloses a plant low-phosphorus-resistant important gene GsMYB7 and application thereof. According to the invention, an MYB family GsMYB7 gene is cloned in soybean for the first time. Researches show that the expression of the GsMYB7 gene is increased under the induction of low phosphorus stress, and under different phosphorus concentration treatment conditions, the overexpression GsMYB7 can obviously increase the biomass of transgenic plants and promote the growth of the plants under the low phosphorus condition; meanwhile, overexpression of GsMYB7 can improve the tolerance of plants to low-phosphorus stress, and reduce the inhibition effect of low phosphorus on plant root growth. Therefore, the GsMYB7 plays an important role in adapting to the low-phosphorus stress of the plant, and the adaptive capacity of the plant to the low-phosphorus stress of the acid soil can be improved through a transgenic technology.

The invention provides a honeysuckle MYB gene family transcription factor 13476. A honeysuckle HQT2 gene, an HQT2 gene promoter and a transcription factor 13476 are cloned respectively, and a yeast one-hybrid experiment proves that 13476 can directly interact with the HQT2 promoter. Furthermore, the regulation of the 13476 protein on the transcriptional activity of the HQT2 gene is analyzed by using a dual luciferase system in tobacco, and the 13476 is proved to have an inhibiting effect on the promoter of the HQT2. Tobacco transient transformation results show that the content of chlorogenic acid in plant cells can be increased by 30-40% through heterologous transient expression of the LjHQT2 gene, and the content of chlorogenic acid reaches the level of 1.59 mg / g. The RNAi method is adopted, molecules aiming at Lj13476 are transiently expressed in honeysuckle, the content of chlorogenic acid can be increased by 10%-20%, the content of isochlorogenic acid A can be increased by 5%-30%, and the content of isochlorogenic acid C can be increased by 7%-20%. The interaction mechanism of the honeysuckle HQT2 gene and the transcription factor 13476 is studied, a certain basis is provided for the regulation mechanism of chlorogenic acid, and the HQT2 gene and the transcription factor 13476 are of great significance for breeding high-quality honeysuckle in the future.

The invention provides application of an MsMYBS3 gene or a biological material thereof in regulation and control of plant cold resistance, and belongs to the technical field of plant genetic engineering. The amino acid sequence coded by the MsMYBS3 gene disclosed by the invention is as shown in SEQ ID NO. 4. The invention provides an application of a medicago sativa MYB transcription factor gene MsMYBS3 in the aspect of regulating and controlling the cold resistance of medicago sativa. A gene editing technology is adopted to directionally change the development and the cold resistance of the medicago sativa. The cold resistance of an MsMYBS3 gene editing strain constructed by the invention is reduced, and the MsMYBS3 gene has the effect of regulating and controlling the cold resistance of alfalfa, so that a theoretical basis and a candidate gene are provided for cultivating new varieties of high-resistance leguminous forage, and the application of alfalfa in production is promoted.

The invention relates to the technical field of genetic engineering and molecular breeding, in particular to a ramie MYB transcription factor and application thereof. The MYB transcription factor is BnMYB6, the amino acid sequence of the MYB transcription factor is as shown in SEQ ID NO.1, and the gene sequence for coding the amino acid of the MYB transcription factor is as shown in SEQ ID NO.2. The transcription factor protein BnMYB6 can specifically and negatively regulate the biosynthesis of plant lignin, and has application value in the aspects of inhibiting the synthesis of plant lignin, promoting the growth and development of a root system and maintaining related traits such as the activity of the root system under drought stress.

The invention discloses application of apple MdMYB41L in regulation and control of seed size, and belongs to the technical field of plant genetic engineering. According to the invention, an MdMYB41L gene is cloned from apple GL-3, the CDS nucleotide sequence of the MdMYB41L gene is shown as SEQ ID NO: 1, and a plant overexpression vector is constructed. A transgenic plant is obtained by heterologous transformation into arabidopsis thaliana through an agrobacterium tumefaciens-mediated method. Compared with wild arabidopsis thaliana, the weight, the length, the width and the surface area of the transgenic plant seeds are obviously improved. In addition, the cotyledon area of a transgenic plant is obviously higher than that of a wild type. Therefore, the MYB transcription factor MdMYB41L provides a new thought in the aspects of improving the plant seed size and molecular breeding.

To provide methods and compositions for controlling sucker growth in tobacco by altering the expression of different target genes.SOLUTION: A modified tobacco plant comprises a mutation in an endogenous gene encoding polypeptide selected from the group consisting of cyclin, cyclin-dependent kinase (CDK), a CDK inhibitor, MYB, and a WRKY transcription factor, wherein the mutation is not present in the endogenous gene in a control tobacco plant of the same variety, and wherein the modified tobacco plant comprises no suckers or reduced suckers after topping as compared to the control tobacco plant when grown under comparable growth conditions.SELECTED DRAWING: None

The invention relates to the technical field of gene engineering, and provides a lycium ruthenicum MYB transcription factor LrMYB77, the amino acid sequence of which is as shown in SEQ ID NO.3. The invention also provides a gene LrMYB77 for coding a transcription factor, and a recombinant expression vector and host bacteria containing the gene. Meanwhile, the invention further provides application of the transcription factor or the gene in promoting synthesis of lycium ruthenicum anthocyanin and a specific method for increasing the synthesis amount of the lycium ruthenicum anthocyanin. According to the invention, the LrMYB77 gene with a regulation effect on anthocyanin synthesis is screened from lycium ruthenicum for the first time, and experiments verify that the expression or overexpression of the gene in a plant is regulated, so that the synthesis and accumulation of anthocyanin in the plant can be remarkably promoted, and the LrMYB77 gene has important significance on germplasm resource creation and molecular breeding of lycium ruthenicum rich in anthocyanin.

The application belongs to the field of plant biotechnology, and particularly relates to a cucumber CsODO1 gene and application thereof, and is a MYB transcription factor capable of being combined with a CsCSE1 promoter, which is screened through a yeast single hybridization. It is proved through EMSA and tobacco transient expression analysis that the CsODO1 gene directly and specifically combines with a "CAACCA" sequence in the CsCSE1 promoter, and inhibits the expression of the CsCSE1 gene. The CsODO1 gene can be induced to express by a powdery mildew fungus, and is differentially expressed in the cucumber infected by the powdery mildew fungus. It is found through a cucumber cotyledon transient transformation system mediated by an agrobacterium that the CsODO1 gene negatively regulates the biosynthesis of lignin and the resistance of the cucumber to the powdery mildew fungus. The application provides a new reference gene resource for the cultivation of a cucumber disease-resistant variety, and provides a new thought for the research on the molecular mechanism of the plant immunity mediated by the lignin pathway.

This invention discloses a gene, AcMYB22, that regulates the biosynthesis of flavonoids in kiwifruit and its applications, belonging to the field of genetic engineering technology. This invention clones two alleles of the AcMYB22 transcription factor, AcMYB22-1 and AcMYB22-2, from the 'Hongyang' kiwifruit and its bud mutations. Plant expression vectors of AcMYB22-1 and AcMYB22-2 were constructed, and overexpressed kiwifruit fruits and stably overexpressed kiwifruit plants were obtained using Agrobacterium-mediated transformation. The overexpressed materials showed a promotion of flavonoid and anthocyanin accumulation. This research enriches the mechanism by which MYB transcription factors regulate the biosynthesis of flavonoids in kiwifruit, provides technical support for improving plant flavonoid synthesis, and is of great significance for the quality improvement and new variety breeding of kiwifruit.

The invention discloses a molecular marker of a wheat MYB transcription factor family member TaRVE1-6B and application of the molecular marker, and belongs to the technical field of crop seed selection and cultivation. The molecular marker comprises RVE1-6B-527566461, RVE1-6B-527565157 and RVE1-6B-527561616, the genotypes of the InDel2 site, the InDel5 site and the InDel8 site can be determined according to the size of an amplification band of the molecular marker, and the haplotype of TaRVE1-6B can be determined according to the genotypes of the InDel2 site, the InDel5 site and the InDel8 site. The molecular marker has the beneficial effects that an excellent allele of TaRVE1-6B is explored from a natural population of wheat, and the molecular marker for identifying the plant height, the ear length and the thousand seed weight of wheat is developed, so that a gene resource and an effective way are provided for genetic improvement of wheat yield traits.

The present invention discloses the application of tomato transcription factor SlMYB1 in regulating tomato fruit quality, Botrytis cinerea resistance and changing fruit shape, belonging to the field of biotechnology. The research of the present invention finds that overexpressing the transcription factor SlMYB1 gene in the fruits of wild-type tomato MicroTom, the fruit color of the tomato overexpression lines is more red and bright at the red-ripe stage, and the top of the fruit is smoother. By measuring the fruit quality, it is found that the contents of secondary metabolites such as vitamin C, carotenoids and flavonoids increase. At the same time, by identifying the Botrytis cinerea resistance of the fruits at the red-ripe stage, it is found that the resistance of the SlMYB1 overexpression lines is enhanced. In summary, the tomato transcription factor SlMYB1 gene positively regulates the quality of tomato fruits and the resistance to Botrytis cinerea, as well as changes the fruit shape, providing a basis for further in-depth study of the function of MYB transcription factors.

Disclosed are use of the Camellia sinensis (L.) O. Kuntz CsMYB1 gene in improving disease resistance of a plant and a method for improving disease resistance of a plant. The CsMYB1 gene is a transcription factor gene of the MYB family. CsMYB1 is constructed into a plant expression vector, and the vector is transferred into Nicotiana benthamiana. A transgenic tobacco plant is obtained by means of stable genetic transformation, and the plant exhibits relatively strong fungal inhibitory activity against inoculated Botrytis cinerea hyphae. Transient silencing of the CsMYB1 gene of Camellia sinensis (L.) O. Kuntz leaves using a technology of antisense oligonucleotides (AsODNs) significantly reduces the disease resistance of the plant. Therefore, the CsMYB1 gene has the effect of improving the disease resistance of a plant and can be used as a disease-resistant gene. Introducing the gene into plants such as tobacco, Camellia sinensis (L.) O. Kuntz, or vegetables can improve the disease resistance of these plants. The present invention has broad market application prospects.

The invention belongs to the technical field of molecular biotechnology and plant genetic engineering, and particularly relates to a grape MYB transcription factor VvMYB108 and application thereof. The invention provides an MYB transcription factor VvMYB108 for regulating and controlling biological stress in a postharvest storage process of grapes. An overexpression strain of the VvMYB108 in arabidopsis thaliana is utilized to find that the sensitivity of a VvMYB108 transgenic strain to botrytis cinerea is enhanced. By using an overexpression strain of the VvMYB108 in arabidopsis thaliana, it is found that the disease resistance of the VvMYB108 transgenic strain to Pst DC3000 is enhanced.

The invention provides a primer group, a method and a kit for detecting thalassemia, which can be used for simultaneously detecting thalassemia related genes, related regulatory regions and modified gene mutations. According to the method, multiple regions and multiple mutations of HBA1, HBA2, HBB and other genes, related regulatory regions (LCR, 3 'HS, HS-40 and MCS-R) and modified genes (HBD, MYB, BCL11A and KLF1) are detected at the same time based on multiple PCR amplification and three-generation sequencing, amplification of related gene deletion type and non-deletion type mutations can be achieved at the same time in a single reaction tube through multiple PCR; a third-generation sequencing platform (an ONT sequencing platform and a PacBio company sequencing platform) is subsequently adopted for subsequent detection, the method is easy and convenient to operate, multiple PCR and a third-generation sequencing library are reliable in quality and high in repeatability, application of the third-generation sequencing technology to clinical detection is facilitated, and the method is suitable for clinical detection. According to the invention, various deletion and non-deletion mutation, complex structure variation and the like can be detected at the same time; when two or more mutations exist on the HBA1 / 2 or HBB gene locus at the same time, cis-mutation or trans-mutation can be distinguished.

The application discloses a gene related to osmanthus fragrans carotenoid synthesis OfPHL5 and application thereof OfPHL5 The gene is a MYB transcription factor gene, and an overexpression vector containing the gene OfPHL5 is transformed into osmanthus fragrans petals to perform transient expression. OfPHL5 The results show that the gene expression amount of the transiently transformed osmanthus fragrans petals is significantly increased, and the total carotenoid content in the petals is increased, which indicates that the gene plays an important role in promoting osmanthus fragrans carotenoid synthesis. OfPHL5 The gene can be used for improving the color quality of osmanthus fragrans and excellent variety breeding in osmanthus fragrans genetic engineering.

The invention relates to application of a coding gene of a corn MYB transcription factor ZmMYBIF35 in regulation and control of plant drought tolerance, and belongs to the technical field of gene engineering and crop genetic breeding, the CDS sequence of the coding gene of the transcription factor ZmMYBIF35 is as shown in SEQ ID NO.1, the genome sequence is as shown in SEQ ID NO.2, the gene is used for positively regulating and controlling the drought tolerance of crop plants, and the amino acid sequence of the transcription factor ZmMYBIF35 is as shown in SEQ ID NO.3. The invention also relates to application of the coding gene of the corn MYB transcription factor ZmMYBIF35 in regulation and control of plant drought tolerance. The ZmMYBIF35 gene overexpression corn plant is analyzed, it is found that the ZmMYBIF35 gene can regulate and control the drought tolerance of the plant, ZmMYBIF35 gene overexpression can enhance the drought tolerance of the plant, and important theoretical significance and application value are provided for creation of new corn germplasm and corn molecular breeding.

The invention discloses an HAPP gene and application thereof, and relates to the field of plant genetic engineering, the identified rice HAPP gene can increase the rice plant height and the seed size at the same time, and after the rice HAPP gene is fused with a PANDA gene, the rice plant height and the seed size can be remarkably increased, but the ear number is reduced. Meanwhile, analysis finds that HAPP originates from a Harbinger transposon Myb-like DNA binding protein, interacts with PANDA and is related to a gene expression mode, but has antagonism in function. Therefore, identification of the HAPP gene not only reveals a new function of the transposon-derived gene in regulation and control of rice yield traits, but also provides new theoretical support and practical guidance for rice breeding.

This invention discloses a wheat blue-grain gene and its applications, belonging to the fields of plant molecular biology, biochemistry, genetics, and plant breeding. Using differential expression analysis between blue-grain and white-grain wheat, four genes controlling the blue-grain trait were identified: two MYB family transcription factors and two bHLH family transcription factors. Furthermore, plant recombinant expression vectors for these genes and a method for regulating anthocyanin synthesis in plants are provided. This has significant theoretical and practical implications for studying the aleurone layer pigment synthesis pathway in blue-grain wheat, its use as a screening marker in plant transformation processes, and improving the nutritional value of plants.

The invention relates to a primer and a probe for quantitatively detecting MYB-PLAGL1 and PLEKHG1-ABL1 fusion genes, a kit and a use method of the kit. The kit is characterized in that 1, the kit comprises an MYB-PLAGL1 primer, a PLEKHG1-ABL1 primer and an ABL1 internal reference primer; the nucleotide sequences of the MYB-PLAGL1 primer comprise SEQ ID NO: 1 and SEQ ID NO: 2, and the nucleotide sequences of the PLEKHG1-ABL1 primer comprise SEQ ID NO: 3 and SEQ ID NO: 4. The invention finds and verifies that a T-ALL patient carries two new fusion genes of MYB-PLAGL1 and PLEKHG1-ABL1, and the fusion genes can be used as molecular markers of the T-ALL patient and are applied to disease diagnosis, treatment scheme selection and minimal residual focus monitoring.

The invention discloses a tea tree MYB transcription factor CsMYB330 gene and application, the nucleotide sequence of the MYB transcription factor CsMYB330 gene is shown as SEQ ID NO: 1, the expression of the gene participates in regulating the flavonol content in plants, and the instantaneous silencing of the CsMYB330 gene in the tea tree can significantly reduce the flavonol content in the tea tree by 24.5% and reduce the bitter taste of the finished tea.

The invention relates to the technical field of plant molecular biology, in particular to application of OsMYB52 protein or a coding gene of the OsMYB52 protein or a biological material containing the coding gene of the OsMYB52 protein to regulation and control of the heading stage of plants. The invention discloses an MYB transcription factor OsMYB52 for negative regulation and control of the heading stage of rice. The gene interacts with Hd1 to enhance the transcriptional activation effect of Hd1 on Ghd7 and Ghd7.1 and inhibit the transcriptional activation of Hd1 on Ehd1 and Hd3a, so that the heading stage of rice is negatively regulated, and the rice yield is changed. The function and the regulation mechanism of the OsMYB52 for regulating the heading stage of the rice are reported for the first time, and the OsMYB52 has important guiding significance for perfecting the molecular mechanism of a rice flowering regulation network and improving the yield and the quality of the rice.

The present application relates to the technical field of plant molecular biology, and particularly relates to application of OsMYB52 protein, or a coding gene thereof, or a biological material containing the coding gene of the OsMYB52 protein in regulating a heading stage of a plant. The present application discloses a MYB transcription factor for negatively regulating a heading stage of rice OsMYB52 . The gene enhances transcription activation of Hd1 on Ghd7 , Ghd7.1 by interacting with Hd1, inhibits transcription activation of Hd1 on Ehd1 , Hd3a , and further negatively regulates the heading stage of rice and causes change of yield of rice. OsMYB52 The function and regulation mechanism of regulating the heading stage of rice are reported for the first time, and the function and regulation mechanism have important guiding significance for perfecting a molecular mechanism of a flowering regulation network of rice and improving yield and quality of rice.