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161 results about "High homology" patented technology
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Nucleic acid aptamer and screening method thereof, and application of nucleic acid aptamer in prostate cancer cell strain detection
ActiveCN103642810AHigh affinityImprove featuresMicrobiological testing/measurementIndividual particle analysisProstate cancer cellChemical synthesis
The present invention discloses a nucleic acid aptamer, wherein the sequence of the nucleic acid aptamer comprises a DNA segment represented by any one sequence selected from a sequence 1, a sequence 2 and a sequence 3. The nucleic acid aptamer can further be various similar sequences with high homology or a derivative obtained from the sequence of the present invention. The invention further discloses a nucleic acid aptamer screening method, which comprises: synthesizing a random single-stranded DNA library and primers, carrying out SELEX screening, carrying out PCR amplification of library, preparing a DNA single strand library, and finally carrying out repeated screening, negative screening and multi-round screening to obtain the nucleic acid aptamer. The nucleic acid aptamer and the derivative thereof can be used in recognition of the prostate cancer cell strain PC-3 or preparation of kits, molecular probes and targeted mediums for prostate cancer detection, and can further be used in design and preparation of prostate cancer treatment drugs. Compared with the protein antibody, the nucleic acid aptamer of the present invention has advantages of high affinity, high specificity, no immunogenicity, capability of being chemically synthesized, small molecular weight, stability, easy storage, easy labeling and the like.
Owner:GUANGZHOU SHIWEN BIOTECHNOLOGY CO LTD
Plant flavonoid synthesis regulation gene and its application
Owner:WUHAN BOTANICAL GARDEN CHINESE ACAD OF SCI
Urocortin-III and uses thereof
A search of the public human genome database identified a human EST, GenBank accession number AW293249, which has high homology to known pufferfish urocortin sequences. The full length sequence was amplified from human genomic DNA and sequenced. Sequence homology comparisons of the novel sequence with human urocortin I and urocortin II revealed that the sequence encoded a novel human urocortin, which was designated urocortin III (UcnIII). While urocortin III does not have high affinity for either CRF-R1 or CRF-R2, the affinity for CRF-R2 is greater than the affinity for CRF-R1. Urocortin III is capable stimulating cyclic AMP production in cells expressing CRF-R2α or β. Thus, the affinity is high enough that urocortin III could act as a native agonist of CRF-R2. However, it is also likely that urocortin III is a stronger agonist of a yet to be identified receptor.
Owner:RES DEVMENT FOUND
Alkaline protease
The present invention relates to an alkaline protease having a prepro sequence, wherein the prepro sequence is a mutant sequence of the amino acid sequence of SEQ ID NO: 1 or an amino acid sequence having 80% or higher homology to the amino acid sequence of SEQ ID NO: 1, in which amino acid residues at (a) position 52, (b) position 75, and (c) position 142 of SEQ ID NO: 1, or amino acid residues at positions corresponding to these positions are substituted by the following amino acid residues:at position (a): aspartic acid or arginine,at position (b): alanine or arginine, andat position (c): lysine;and the alkaline protease, when in a mature form, has the amino acid sequence of SEQ ID NO: 2 or an amino acid sequence having a homology of 80% or higher to this amino acid sequence. The present invention also relates to, for example, a gene encoding the alkaline protease.According to the present invention, an alkaline protease increased in production can be produced. In particular, there can be efficiently produced an alkaline protease having an activity even in the presence of a fatty acid of high concentration and exhibiting excellent detergency against complex soils containing proteins and sebum.
Owner:KAO CORP
Keratinase mutant with improved thermal stability and preparation method thereof
ActiveCN104017791AImprove thermal stabilityIncrease enzyme activityBacteriaHydrolasesWater insolubleHigh homology
The invention discloses a keratinase mutant with improved thermal stability and a preparation method thereof, and belongs to the field of enzyme engineering. Thermal stability and substrate specificity of the keratinase are improved by interchanging N ends or C ends of two different keratinases with higher homology from the same bacterial strain. The keratinase and the mutant thereof can effectively hydrolyze water-insoluble keratinase substrates such as feathers, wools, and the like, and can be used for leather spinning industry and feed industry.
Owner:JIANGNAN UNIV
LncRNA and application thereof as detection marker or prognosis recurrence marker for breast cancer combined with lung cancer
InactiveCN107267606ALarge amount of informationComprehensive direct accessMicrobiological testing/measurementDNA/RNA fragmentationHigh homologyLung cancer
The invention discloses lncRNA and application thereof as a detection marker or a prognosis recurrence marker for breast cancer combined with lung cancer. The lncRNA is at least one of the following nucleic acid molecules: (1) nucleic acid molecules with cDNA sequences as shown in SEQ ID NO.1, SEQ ID NO.2 or SEQ ID NO.3; and (2) nucleic acid molecules with 90% and higher homology with the nucleic acid molecules with the cDNA sequences as shown in the SEQ ID NO.1, SEQ ID NO.2 or SEQ ID NO.3. According to the lncRNA and application thereof, a group of lncRNAs are screened out as the molecular markers for the breast cancer combined with lung cancer, associated primers are optimized for preparing primer probe chips, and the lncRNA has the advantages of low detection cost, high sensitivity and the like; and the lncRNA and application thereof take the breast cancer combined with lung cancer, such combined cancer, as an example, and take the lead in filling up the blank in research of the combined biological marker lncRNA in China. The lncRNA and application thereof adopt a group of lncRNAs as associated biological markers for combined cancer, and can effectively improve the clinical diagnosis and prognosis evaluation of the combined cancer for instructing clinical treatment.
Owner:ZHEJIANG SCI-TECH UNIV
Application of utilizing beta-phenylalanine compounds as aldose reductase inhibitors
InactiveCN102512407AHigh selectivityLow toxicityOrganic active ingredientsSenses disorderDiabetic complicationPhenylalanine
The invention discloses an application of utilizing beta-phenylalanine compounds as aldose reductase inhibitors. The beta-phenylalanine compounds are used as aldose reductase (ALR2) inhibitors, have the similar enzymology activity of medicine Epalrestat on the market, and show the high selectivity to aldose reductase (EC1.1. 1.2, ALR1) which has very high homology to the ALR2, the cytotoxicity test shows that the compounds have small toxicity. The compounds can be used for preparing medicines for curing or preventing diabetic complications (diabetic cataract, peripheral neuropathy, retinopathy, kidney disease and arterial atherosclerosis). The general formula of the skeleton structure of the beta-phenylalanine compounds is shown in the description: wherein R1, R4 and R5 are hydrogen; R2 and R3 are hydrogen, alkyl, alkoxy, sulfhydryl or halogen; and R6 is naphth, pyrimidyl, pyrazinyl, phenyl, tetrahydro naphthyl or indolyl.
Owner:广州市爱菩新医药科技有限公司
Diabetes treatment composition based on traditional Chinese medicine nutritional therapy and application thereof
ActiveCN110200276ALower blood sugar levelsAchieve low GIDough treatmentBakery productsAdditive ingredientLow calorie
The invention relates to a diabetes treatment composition based on traditional Chinese medicine nutritional therapy and application thereof, wherein the diabetes treatment composition mainly comprisesvarious natural materials including medicinal and edible materials. The diabetes treatment composition is a low-GI composition, comprising various compound ingredients which may present through carbohydrates, fats and dietary fiber; by usage each day, the dietary fiber in the composition accounts for 10-40 g; the diabetes treatment composition has the total energy of 400-1300 calories, wherein energy provided according to the present quantity of carbohydrates is 15-45% of the total energy of the composition herein; energy provided according to the present quantity of fat is 40-80% of the total energy of the composition, wherein the mass of unsaturated fatty acids is 50-80% of total mass of fat. The composition provided herein lays emphasis on the here-low three-high structure of low GI, low calorie, low available carbohydrates, high unsaturated fatty acids, high dietary fiber and high homology of medicine and food; the intermittent fasting means helps reduce the glucose level of a diabetic patient effectively.
Owner:刘东波
Syphilis spirochete membrane antigen with shorten expression and uses thereof
InactiveCN101293919ASignificant antigen reactivityAntigen reactivity verificationBacteriaDepsipeptidesAntigenSpiroplasma
The invention discloses a DNA sequence expressing a truncated treponema pallidum membrane antigen and an amino acid sequence The membrane antigen is removed of the part having high homology with human fibronectin, so as to avoid false positive and improve the specificity of the serological test for diagnosis of treponema pallidum infection. The invention also discloses the application of the membrane antigen in preparing diagnostic reagents for detecting treponema pallidum infection.
Owner:ARMY MEDICAL UNIV
Brassica napus, parent species Chinese cabbage, cabbage MYBL2 (v-myb avian myeloblastosis viral oncogene homolog-like 2) gene family and application thereof
InactiveCN102226193AReduced seed coat pigmentSeed coat thinningFermentationVector-based foreign material introductionBrassicaHigh homology
The invention discloses a Brassica napus, parent species Chinese cabbage, cabbage MYBL2 (v-myb avian myeloblastosis viral oncogene homolog-like 2) gene family. The Chinese cabbage MYBL2 gene family comprises a BrMYBL2-1 gene and a BrMYBL2-2 gene. The cabbage MYBL2 gene family comprises a BoMYBL2-1 gene and a BoMYBL2-2 gene. The Brassica napus MYBL2 gene family comprises a BnMYBL2-1 gene, a BnMYBL2-2 gene, a BnMYBL2-3 gene, a BnMYBL2-4 gene. Eight MYBL2 genes have higher homology. The invention also discloses the application of the above gene families in molecular breeding with characters, such as plant seed coat color, seed coat thickness and the like. After a just overexpression plant carrier is built and double 10 number in the black Brassica napus variety, the Brassica napus is compared with the contrast of the explant of a non-transgenosis, transgenosis plants normally grow and develop, pigments such as Proanthocyanidins in the seed coat are reduced, and meanwhile the seed coat becomes thin to form the transgenosis yellow seed character.
Owner:SOUTHWEST UNIVERSITY
Lipases with high specificity towards short chain fatty acids and uses thereof
ActiveUS20110262591A1Improves inventionIncrease production levelsFungiBacteriaCooking & bakingHigh homology
The present invention relates to novel polynucleotide sequences comprising genes that encode novel lipolytic enzymes, as well asfunctional equivalents of the gene or the amino acid sequences with high homology thereto. The invention also relates to methods of using these lipolytic enzymes in industrial processes, for example in the dairy or baking industry.
Owner:DSM IP ASSETS BV
Step-up method for cold-pcr enrichment
InactiveUS20130309724A1Minimizing well-to-well variationAvoid differencesMicrobiological testing/measurementFermentationEnrichment methodsHigh homology
Methods of using polymerase chain reactions to enrich a target sequence in a sample containing reference sequences and target sequences having high homology and amplifiable by the same primer pair are provided herein. In particular the methods provide a robust means to improve the fold enrichment of the target sequence and minimize reaction-to-reaction, well-to-well and run-to-run variations in the enrichment methods.
Owner:TRANSGENOMIC
Bridge type fluorescent probe with bridge type sequence zone doping into mismatched bases and application and method
InactiveCN105274096ASimple designHigh detection sensitivityMicrobiological testing/measurementLuminescent compositionsFluorescenceBridge type
The present invention discloses a bridge type fluorescent probe with a bridge type sequence zone doping into mismatched bases and application and a method, the bridge type fluorescent probe 5'-end to 3'-end nucleic acid sequence successively comprises a recognition sequence zone complementary to a nucleic acid sequence of a to-be-tested mutant gene, the bridge type sequence zone, and an anchor sequence zone, wherein the anchor sequence zone is complementary to the outer side sequence zone of 3'-end of the to-be-tested mutant gene, the 3'-end of the anchor sequence is connected with an extension blocking group ; the bridge type sequence zone is a nucleotide derivative sequence containing at least one mismatched bases, an interactiveness fluorescent marking system is marked between the recognition sequence zone and the bridge type sequence zone, the bridge type fluorescent probe is simple in design, can specifically recognizes the gene, is high in sensitivity, and can be used to detect single base mutation and identify highly homologous species and subtypes.
Owner:THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA +1
Novel fructosyl peptide oxidase
ActiveUS20120003678A1Strong specificityStable fructosyl peptide oxidase activityFungiBacteriaEscherichia coliHigh homology
A protein described in any one of [1] to [4] below is provided according to the present invention:[1] a protein comprising the amino acid sequence represented by SEQ ID NO: 1;[2] a protein comprising an amino acid sequence with one or more amino acid deletions, substitutions, or additions in the amino acid sequence represented by SEQ ID NO: 1, and having fructosyl peptide oxidase activity;[3] a protein comprising an amino acid sequence having 80% or higher homology to the amino acid sequence represented by SEQ ID NO: 1, and having fructosyl peptide oxidase activity; and[4] a protein having fructosyl peptide oxidase activity, which is produced by an expression plasmid harbored by the Escherichia coli XL1-Blue MRF' strain deposited under Accession No. FERM BP-11026.
Owner:KYOWA MEDEX CO LTD
Rice temperature sensitive male sterility gene, coding protein and application thereof
InactiveCN102121017AWith temperature sensitive characteristicsPlant peptidesFermentationAgricultural scienceNucleotide
The invention relates to a rice temperature sensitive male sterility gene, a coding protein and application thereof. The rice temperature sensitive male sterility gene has one of the following nucleotide sequences, i.e. 1, a nucleotide sequence shown in SEQ IN NO1, and 2, a NDA sequence which is shown in SEQ IN NO3, has highest homology with a base sequence of an arabidopsis gene and an expected value of 1.8e-27 and is used for coding same biological functional proteins. The transcription expression of the gene OsATMS1 has a characteristic of being sensitive to the environment temperature, can ensure that the plant male fertility has a temperature sensitive characteristic, is expected to have an application value on two aspects of crop crossbreeding and yield increasing.
Owner:SHANGHAI UNIV
Protein-ligand binding site predicting method based on inquiry drive
InactiveCN103617203ATargetedEfficient use ofBiostatisticsSpecial data processing applicationsData setBinding site
The invention provides a protein-ligand binding site predicting method based on inquiry drive. The method includes the steps of firstly, for an given inquiry input, searching protein sequences with high homology to form a training data set based on inquiry drive; secondly, extracting all the binding residues in the training data set as the positive sample set and extracting all the non-binding residues in the training data set as the negative sample set; thirdly, extracting the feature vector of each sample from evolution information and secondary structure perspective to obtain the feature vector sets of the positive and negative samples; fourthly, using a standard support vector machine algorithm for training to obtain an SVM prediction model based on the inquiry input q; fifthly, for the inquiry input, using the same feature extracting method to extract the feature vector of each residue, inputting the feature vector of each residue into the SVM prediction model, and predicting by using a threshold segmentation method. By the method, prediction precision can be increased, and the possible problems of over-optimization and over-fitting on the fixed training data set can be prevented.
Owner:NANJING UNIV OF SCI & TECH
Step-up method for COLD-PCR enrichment
InactiveUS9133490B2Limit difference in temperatureRobust and amenable to useMicrobiological testing/measurementFermentationEnrichment methodsHigh homology
Methods of using polymerase chain reactions to enrich a target sequence in a sample containing reference sequences and target sequences having high homology and amplifiable by the same primer pair are provided herein. In particular the methods provide a robust means to improve the fold enrichment of the target sequence and minimize reaction-to-reaction, well-to-well and run-to-run variations in the enrichment methods.
Owner:TRANSGENOMIC
Homology retrieval system, homology retrieval apparatus, and homology retrieval method
InactiveUS20100205204A1Improve accuracyExact searchDigital data information retrievalDigital data processing detailsGenome scaleHigh homology
A homology retrieval can be performed with higher accuracy than conventional technologies when comparing a query sequence with a target sequence, and retrieving a similar location in the target sequence. The sequence information of a query sequence and a genomic-scale target sequence is acquired, the acquired information is compressingly converted into a compressed query sequence and a compressed target sequence in each of which a homopolymer region including two or more consecutive identical bases is replaced with a single base of the bases, the two sequences are compared, and a refining search is performed for a compressed target partial sequence that matches the compressed query sequence in the compressed target sequence. For the refined compressed candidate sequence and the query sequence, based on the information on the number of consecutive identical bases in the each of the sequences before compression, the number of consecutive bases is compared between the two compressed sequences for each corresponding base, and the degree of similarity indicating homology of the candidate sequence with the query sequence is computed from a degree of match or a degree of mismatch in the number of consecutive bases. By ranking and selecting an arbitrary number of candidate sequences having relatively high homology with the query sequence from this degree of similarity, it is possible to avoid the influence of the number of consecutive identical bases in a homopolymer region, thereby performing a homology retrieval accurately.
Owner:INTER UNIV RES INST RES ORG OF INFORMATION & SYST
Maize gene ZmGRAS37 for regulating and controlling nutrient body largeness, early blossoming and grain weight increment of arabidopsis thaliana at seedling stage and application thereof
Owner:SHANDONG AGRICULTURAL UNIVERSITY
Method for cloning rice auxin induced protein gene
InactiveCN101492671AHigh homologySimple implementation stepsFermentationPlant genotype modificationMutantDNA Intercalation
The invention relates to a method for using rice auxin to induce protein genes to be cloned, which is characterized in that the implementation steps comprise: (1) the preparation of a rice transformation receptor; (2) the genetic transformation of the rice; (3) the screening of kanamycin-resistant callus tissue and the regeneration of plants; (4) the screening of the mutant of a T-DNA inserted progenies; (5) Tail-PCR; (6) the comparison and analysis of sequences on the Internet. In the invention, a rice mutant with a short plant height is obtained when carrying out rice functional genome research by using T-DNA label method; the lateral neighboring sequences of the mutant are researched by using TAIL-PCR technology; meanwhile, the position where the mutant T-DNA inserts the rice genome is arranged on the No. 4 chromosome of the rice by the comparison on the databases of NCBI and TIGR on the Internet; moreover, the rice BAC clone (OSJNBa0084K01) of the position is found out. The T-DNA is inserted between the two genes of the clone by analyzing the clone. Known functional genes with a very high homology with BAC cloning code amino acid sequence are forecasted by the sequence comparison on the Internet.
Owner:TIANJIN AGRICULTURE COLLEGE
Maize chloroplast protein synthesis elongation factors and methods of use for same
InactiveUS7388125B2Improve stabilityImprove toleranceSugar derivativesOther foreign material introduction processesBiotechnologyBreeding program
Owner:THE UNIV OF SOUTH DAKOTA +2
Predictive biomarker PD-L2 targeted polypeptide for tumor immunotherapy and application of predictive biomarker PD-L2 targeted polypeptide
ActiveCN107556367ATransport stableIncrease contentBiological testingMacromolecular non-active ingredientsClinical efficacyClinical value
The invention relates to a predictive biomarker PD-L2 targeted polypeptide for tumor immunotherapy and an application of the predictive biomarker PD-L2 targeted polypeptide. The general formula of thepolypeptide is: HX1RX2X3X4X5RIRN. The invention also relates to a DNA fragment encoding the polypeptide, an expression vector expressing the polypeptide, a host cell and a bivalent, a multivalent orpharmaceutical composition formed from the polypeptide. The polypeptide can be used for detecting the expression of PD-L2 in tumor cells and immune cells, can be taken as a detection index for predicting the clinical efficacy of anti-PD-L1 / PD-L2-PD-1 therapy and can specifically distinguish PD-L1 and PD-L2 protein with high homology, thereby having great clinical value and significance for optimization of therapeutic schedules and curative effect monitoring of individual patients.
Owner:THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
EPSP synthase gene derived from ochrobactrum anthropi and application thereof
InactiveCN102108363AImprove toleranceTransferasesMicroorganism based processesNucleotideGenetically modified crops
The invention discloses an EPSP synthase gene derived from ochrobactrum anthropi and application thereof. The EPSP synthase gene contains 1353 basic groups and 451 coded amino acids, the nucleotide sequence of the EPSP synthase gene is as shown in SEQ ID NO1, and the amino acid sequence of the coded proteins is as shown in SEQ ID NO2. The EPSP synthase gene derived from ochrobactrum anthropi disclosed by the invention is synthesized by a manual method, has high homology with the reported EPSP synthase gene derived from agrobacterium tumfaciens, has higher glyphosate tolerance, and can be used for culturing genetically modified crops.
Owner:SHANGHAI ACAD OF AGRI SCI
Sheep coenurus skrjabini specific gene and its separation and identification
InactiveCN101070539AProvoke an immune responseHigh homologyBacteriaMicrobiological testing/measurementProtein moleculesLarva currens
This invention relates to one kind of sheep multi-thread you sickness specificity gene and the separation and the appraisal, the design directs the thing, with the RT-PCR method, the larva stage which the sheep multi-thread you get sick separates the new gene, its naming is 45m, its length is 698bp, codes 232 amino acids. This gene and the sheep belt tapeworm, the cow belt tapeworm's protection gene piece has the very high homology. The quantity of protein molecule expressed is approximately 6,300 road Er pause. Swells 45 milligrams to pET41b in the expression vector, the construction tallies reads the code frame's fusion gene, the expression quantity may reach 100-120mg / L . Uses in sheep's immunising experiment this protein, may arouse organism effectively the immune response, its antiserum may has the specificity response with the prosopon and the larva antigen total protein in 63KD place , It is thought that this gene has the possibility to become the candidate vaccine member which the multi-thread you get sick.
Owner:新疆维吾尔自治区畜牧科学院兽医研究所
Aptamer of docetaxel, aptamer derivative and use thereof
ActiveCN103898119AHigh affinityStrong specificityOrganic active ingredientsPharmaceutical non-active ingredientsChemical synthesisAptamer
The invention discloses an aptamer of docetaxel, an aptamer derivative and use thereof. The aptamer sequence of the docetaxel comprises a DNA segment shown in a sequence 1 or a sequence 2. The aptamer can be the derivative obtained from various similar sequences with high homology or the sequence disclosed by the invention. The aptamer and the derivative thereof disclosed by the invention can be used as drug carriers, or applied to drug design and development, drug separation and purification, preparation of drugs and other products, preparation of docetaxel detection probes or target probes and the like. Therefore, the aptamer and the derivative thereof have the advantages that the aptamer and the derivative thereof can be combined with high specificity and high affinity of the docetaxel, can be subjected to chemical synthesis, and is free of immunogenicity, good in biocompatibility, small and stable in molecular weight, easy to store and the like.
Owner:HUNAN UNIV
Hydrazine cathepsin K inhibitor and application thereof in treating osteoporosis
InactiveCN103086923AHigh selectivityHigh yieldOrganic chemistryAntipyreticCathepsin KHydrazine compound
The invention provides a hydrazine cathepsin K inhibitor and an application of the inhibitor in treating osteoporosis, which belong to the technical field of cathepsin inhibitors. The inhibitor does not include a P2-P3 connection part but have different P3 locus structures. The structure is represented by the following formula, and in the formula, P3 is aryl and comprises para-biphenyl, para-phenyl-pyridine, para-phenyl thiophene, para-terphenyl and meta-terphenyl. The non-peptide hydrazine cathepsin inhibitor designed and synthesized in the invention has the advantages of being relatively simple in structure, easy to get in materials, convenient to synthesize and high in yield; the design and the synthesis of the matrix structure greatly facilitate the synthesis of subsequent final products. By adopting the inhibitor, the inhibiting effect to the cathepsin K is higher than sub-nano-mole level, and the inhibiting effect to B and S is on micro-mole level, and the difference of inhibiting effects of cathepsin K and cathepsin L with extremely high homology is increased, thus the possibility of developing the inhibitor as the medicine for treating osteoporosis is increased.
Owner:JILIN UNIV
Magnaporthe grisea MoLON1 gene function and application thereof
The Magnaporthe grisea MoLON1 gene function and application thereof belong to the field of bioengineering technology, and the invention relates to the function in the pathogenic processes of Magnaporthe grisea MoLON1 gene and purification and purpose of coding proteins thereof. The gene and the ATP-dependent protease Lon1 in yeast, Escherichia coli and other organisms have high homology, and the gene codes 1119 amino acids and contains one intron and two exons. The knockout of MoLON1 gene leads to minimizing of Magnaporthe grisea aerial hyphae quantity, reduction of conidiospore output, basic deprivation of expansion capability of infective hyphae in the paddy rice leaf cells, and improvement of sensitivity to a plurality of adverse conditions; the invention also relates to expression and purification of proteins coded by the MoLON1 gene, and gene can be used as a candidate target for designing and screening anti-Magnaporthe grisea novel medicament.
Owner:JILIN UNIV
Functional humanization of complementarity determining regions (CDRS)
InactiveUS20100197896A1Maximizing numberAffecting specificityImmunoglobulinsFermentationComplementarity determining regionImmunglobulin e
Current humanization approaches for immunoglobulins focus mostly on modifying the framework regions into human sequences. Herein is provided a method for humanizing antibody complementarity-determining regions (CDRs) through functional humanization to reduce the potential immunogenicity of non-human CDR-containing antibodies. CDRs with high sequence homology to the parent CDR are identified from a database of human CDR sequences. One or more human CDRs that are highly homologous to the parent CDR sequence can be used to replace the corresponding CDRs of murine immunoglobulins (or their humanized, or re-engineered versions). Human CDRs that improve or have minimal effects on the antigen binding affinity and specificity are adopted.
Owner:SINOMAB BIOSCI
Cabbage type rape, parent species Chinese cabbage thereof, cabbage TT2 (Transparent Testa 2) gene family and application thereof
The invention relates to the technical field of gene engineering, in particular to cabbage type rape, a parent species Chinese cabbage thereof, a cabbage TT2 (Transparent Testa 2) gene family and application thereof. The Chinese cabbage TT2 gene family comprises two members of a BrTT2-1 gene and a BrTT2-2 gene; the cabbage TT2 gene family has one member of a BoTT2 gene; the cabbage type rape TT2 gene family comprises three members of a BnTT2-1 gene, a BnTT2-2 gene and a BnTT2-3 gene; and the six brassica TT2 genes have higher homology. The invention also discloses the application of the gene family in plant molecular breeding. After the antisense inhibition of the expression of the cabbage type rape in black-seed cabbage type rape, a transgenic plant generates the character variation of testa color lightening, and the like and has potential for creating transgenic yellow-seed materials.
Owner:SOUTHWEST UNIVERSITY
Preparation method for B cell CD 22 extracellular inhibitive peptide fragment B2285 vaccines
InactiveCN101897961AAvoid immunogenicityLess toxic side effectsPeptidesCarrier-bound antigen/hapten ingredientsDiseaseHigh homology
The invention provides a preparation method for B cell CD 22 extracellular peptide fragment B2285 vaccines. Sequential eight amino acids from the 85th to the 93rd starting at an amino terminal in a mice CD 22 molecule amino acid sequence, specifically in a sequence of Lys- Thr- Glu- Lys- Asp-Pro- Glu- Ser- Glu, are selected. The sequence is similar to the sequence of Lys- Asp- Gly- Lys- Val- Pro-Ser- Glu, having the sequential eight amino acids from the 81st to the 88th starting at the amino terminal in a human CD 22 molecular amino acid sequence, is located at the same functional region in a spatial structure, and has high homology. A prepared vaccine or a drug composite containing B2285 has the functions of inhibiting B cell proliferation, activating and generating antibody per se, canbe applicable to treating immunologic diseases involved or mediated B cells such as dilated cardiomyopathy testing positive in an anti-myocardial antibody, systemic lupus erythematosus with a positive antibody per se, and the like.
Owner:XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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