160 results about "High homology" patented technology

The present invention discloses a nucleic acid aptamer, wherein the sequence of the nucleic acid aptamer comprises a DNA segment represented by any one sequence selected from a sequence 1, a sequence 2 and a sequence 3. The nucleic acid aptamer can further be various similar sequences with high homology or a derivative obtained from the sequence of the present invention. The invention further discloses a nucleic acid aptamer screening method, which comprises: synthesizing a random single-stranded DNA library and primers, carrying out SELEX screening, carrying out PCR amplification of library, preparing a DNA single strand library, and finally carrying out repeated screening, negative screening and multi-round screening to obtain the nucleic acid aptamer. The nucleic acid aptamer and the derivative thereof can be used in recognition of the prostate cancer cell strain PC-3 or preparation of kits, molecular probes and targeted mediums for prostate cancer detection, and can further be used in design and preparation of prostate cancer treatment drugs. Compared with the protein antibody, the nucleic acid aptamer of the present invention has advantages of high affinity, high specificity, no immunogenicity, capability of being chemically synthesized, small molecular weight, stability, easy storage, easy labeling and the like.

The invention discloses a plant flavonoid synthesis regulation gene and its application. CDNA obtained through the inverse transcription of the total RNA of Epimedium brevicornum Maxim blades is treated as a template and undergoes PCR to obtain a 233bp fragment, the 233bp fragment has a very high homology with flavonoid related R2R3-MYB gene, a full-length cDNA sequence is obtained through an RACE technology and is named EsMYBA1 gene, and the sequence of the EsMYBA1 gene is represented by SEQIDNO.1. The EsMYBA1 gene codes an R2R3-MYB transcription factor which has an about 50% homology with other MYB regulation genes for controlling the flavonoid synthesis approaches, and the improvement of the expression level of the EsMYBA1 gene through a genetic engineering technology promotes the synthesis and the accumulation of anthocyanidin.

A search of the public human genome database identified a human EST, GenBank accession number AW293249, which has high homology to known pufferfish urocortin sequences. The full length sequence was amplified from human genomic DNA and sequenced. Sequence homology comparisons of the novel sequence with human urocortin I and urocortin II revealed that the sequence encoded a novel human urocortin, which was designated urocortin III (UcnIII). While urocortin III does not have high affinity for either CRF-R1 or CRF-R2, the affinity for CRF-R2 is greater than the affinity for CRF-R1. Urocortin III is capable stimulating cyclic AMP production in cells expressing CRF-R2α or β. Thus, the affinity is high enough that urocortin III could act as a native agonist of CRF-R2. However, it is also likely that urocortin III is a stronger agonist of a yet to be identified receptor.

The invention discloses an application of utilizing beta-phenylalanine compounds as aldose reductase inhibitors. The beta-phenylalanine compounds are used as aldose reductase (ALR2) inhibitors, have the similar enzymology activity of medicine Epalrestat on the market, and show the high selectivity to aldose reductase (EC1.1. 1.2, ALR1) which has very high homology to the ALR2, the cytotoxicity test shows that the compounds have small toxicity. The compounds can be used for preparing medicines for curing or preventing diabetic complications (diabetic cataract, peripheral neuropathy, retinopathy, kidney disease and arterial atherosclerosis). The general formula of the skeleton structure of the beta-phenylalanine compounds is shown in the description: wherein R1, R4 and R5 are hydrogen; R2 and R3 are hydrogen, alkyl, alkoxy, sulfhydryl or halogen; and R6 is naphth, pyrimidyl, pyrazinyl, phenyl, tetrahydro naphthyl or indolyl.

The invention discloses a Brassica napus, parent species Chinese cabbage, cabbage MYBL2 (v-myb avian myeloblastosis viral oncogene homolog-like 2) gene family. The Chinese cabbage MYBL2 gene family comprises a BrMYBL2-1 gene and a BrMYBL2-2 gene. The cabbage MYBL2 gene family comprises a BoMYBL2-1 gene and a BoMYBL2-2 gene. The Brassica napus MYBL2 gene family comprises a BnMYBL2-1 gene, a BnMYBL2-2 gene, a BnMYBL2-3 gene, a BnMYBL2-4 gene. Eight MYBL2 genes have higher homology. The invention also discloses the application of the above gene families in molecular breeding with characters, such as plant seed coat color, seed coat thickness and the like. After a just overexpression plant carrier is built and double 10 number in the black Brassica napus variety, the Brassica napus is compared with the contrast of the explant of a non-transgenosis, transgenosis plants normally grow and develop, pigments such as Proanthocyanidins in the seed coat are reduced, and meanwhile the seed coat becomes thin to form the transgenosis yellow seed character.

The present invention relates to novel polynucleotide sequences comprising genes that encode novel lipolytic enzymes, as well asfunctional equivalents of the gene or the amino acid sequences with high homology thereto. The invention also relates to methods of using these lipolytic enzymes in industrial processes, for example in the dairy or baking industry.

Methods of using polymerase chain reactions to enrich a target sequence in a sample containing reference sequences and target sequences having high homology and amplifiable by the same primer pair are provided herein. In particular the methods provide a robust means to improve the fold enrichment of the target sequence and minimize reaction-to-reaction, well-to-well and run-to-run variations in the enrichment methods.

A protein described in any one of [1] to [4] below is provided according to the present invention:

• • [1] a protein comprising the amino acid sequence represented by SEQ ID NO: 1;
  • [2] a protein comprising an amino acid sequence with one or more amino acid deletions, substitutions, or additions in the amino acid sequence represented by SEQ ID NO: 1, and having fructosyl peptide oxidase activity;
  • [3] a protein comprising an amino acid sequence having 80% or higher homology to the amino acid sequence represented by SEQ ID NO: 1, and having fructosyl peptide oxidase activity; and
  • [4] a protein having fructosyl peptide oxidase activity, which is produced by an expression plasmid harbored by the Escherichia coli XL1-Blue MRF' strain deposited under Accession No. FERM BP-11026.

The invention relates to a rice temperature sensitive male sterility gene, a coding protein and application thereof. The rice temperature sensitive male sterility gene has one of the following nucleotide sequences, i.e. 1, a nucleotide sequence shown in SEQ IN NO1, and 2, a NDA sequence which is shown in SEQ IN NO3, has highest homology with a base sequence of an arabidopsis gene and an expected value of 1.8e-27 and is used for coding same biological functional proteins. The transcription expression of the gene OsATMS1 has a characteristic of being sensitive to the environment temperature, can ensure that the plant male fertility has a temperature sensitive characteristic, is expected to have an application value on two aspects of crop crossbreeding and yield increasing.

The invention provides a maize gene ZmGRAS37 for regulating and controlling the nutrient body largeness, the early blossoming and the grain weight increment of arabidopsis thaliana at a seedling stage and application thereof. According to the maize gene ZmGRAS37, the gene ZmGRAS37 is separated and cloned from maize; the cDNA (complementary Deoxyribonucleic Acid) sequence of the ZmGRAS37 is connected to an expression vector pPZP211 promoted by a 35S promoter; the arabidopsis thaliana is infected and converted by utilizing an agrobacterium infection method; indicated by an experimental result, the gene can be used for enabling the model-plant arabidopsis thaliana to generate characters of the nutrient body largeness and to further have the early blossoming, the grain weight increment and the like at the seedling stage. Shown by a phylogenetic-tree analysis result, the direct homologous gene of the gene does not exist in the model-plant arabidopsis thaliana; however, the gene has the higher homology with that of sorghum and maize of gramineous plants; the gene is accounted to be the peculiar GRAS (Generally Recognized as Safe) family gene of the graminaceous plants; in consideration of the conservatism of the gene in the graminaceous plants and the phenotypes presented in transgenic arabidopsis thaliana, the gene is proven to have potential application value.

The invention relates to a method for using rice auxin to induce protein genes to be cloned, which is characterized in that the implementation steps comprise: (1) the preparation of a rice transformation receptor; (2) the genetic transformation of the rice; (3) the screening of kanamycin-resistant callus tissue and the regeneration of plants; (4) the screening of the mutant of a T-DNA inserted progenies; (5) Tail-PCR; (6) the comparison and analysis of sequences on the Internet. In the invention, a rice mutant with a short plant height is obtained when carrying out rice functional genome research by using T-DNA label method; the lateral neighboring sequences of the mutant are researched by using TAIL-PCR technology; meanwhile, the position where the mutant T-DNA inserts the rice genome is arranged on the No. 4 chromosome of the rice by the comparison on the databases of NCBI and TIGR on the Internet; moreover, the rice BAC clone (OSJNBa0084K01) of the position is found out. The T-DNA is inserted between the two genes of the clone by analyzing the clone. Known functional genes with a very high homology with BAC cloning code amino acid sequence are forecasted by the sequence comparison on the Internet.

The invention relates to a predictive biomarker PD-L2 targeted polypeptide for tumor immunotherapy and an application of the predictive biomarker PD-L2 targeted polypeptide. The general formula of thepolypeptide is: HX1RX2X3X4X5RIRN. The invention also relates to a DNA fragment encoding the polypeptide, an expression vector expressing the polypeptide, a host cell and a bivalent, a multivalent orpharmaceutical composition formed from the polypeptide. The polypeptide can be used for detecting the expression of PD-L2 in tumor cells and immune cells, can be taken as a detection index for predicting the clinical efficacy of anti-PD-L1/PD-L2-PD-1 therapy and can specifically distinguish PD-L1 and PD-L2 protein with high homology, thereby having great clinical value and significance for optimization of therapeutic schedules and curative effect monitoring of individual patients.

This invention relates to one kind of sheep multi-thread you sickness specificity gene and the separation and the appraisal, the design directs the thing, with the RT-PCR method, the larva stage which the sheep multi-thread you get sick separates the new gene, its naming is 45m, its length is 698bp, codes 232 amino acids. This gene and the sheep belt tapeworm, the cow belt tapeworm's protection gene piece has the very high homology. The quantity of protein molecule expressed is approximately 6,300 road Er pause. Swells 45 milligrams to pET41b in the expression vector, the construction tallies reads the code frame's fusion gene, the expression quantity may reach 100-120mg/L . Uses in sheep's immunising experiment this protein, may arouse organism effectively the immune response, its antiserum may has the specificity response with the prosopon and the larva antigen total protein in 63KD place , It is thought that this gene has the possibility to become the candidate vaccine member which the multi-thread you get sick.

The invention discloses an aptamer of docetaxel, an aptamer derivative and use thereof. The aptamer sequence of the docetaxel comprises a DNA segment shown in a sequence 1 or a sequence 2. The aptamer can be the derivative obtained from various similar sequences with high homology or the sequence disclosed by the invention. The aptamer and the derivative thereof disclosed by the invention can be used as drug carriers, or applied to drug design and development, drug separation and purification, preparation of drugs and other products, preparation of docetaxel detection probes or target probes and the like. Therefore, the aptamer and the derivative thereof have the advantages that the aptamer and the derivative thereof can be combined with high specificity and high affinity of the docetaxel, can be subjected to chemical synthesis, and is free of immunogenicity, good in biocompatibility, small and stable in molecular weight, easy to store and the like.

The invention provides a hydrazine cathepsin K inhibitor and an application of the inhibitor in treating osteoporosis, which belong to the technical field of cathepsin inhibitors. The inhibitor does not include a P2-P3 connection part but have different P3 locus structures. The structure is represented by the following formula, and in the formula, P3 is aryl and comprises para-biphenyl, para-phenyl-pyridine, para-phenyl thiophene, para-terphenyl and meta-terphenyl. The non-peptide hydrazine cathepsin inhibitor designed and synthesized in the invention has the advantages of being relatively simple in structure, easy to get in materials, convenient to synthesize and high in yield; the design and the synthesis of the matrix structure greatly facilitate the synthesis of subsequent final products. By adopting the inhibitor, the inhibiting effect to the cathepsin K is higher than sub-nano-mole level, and the inhibiting effect to B and S is on micro-mole level, and the difference of inhibiting effects of cathepsin K and cathepsin L with extremely high homology is increased, thus the possibility of developing the inhibitor as the medicine for treating osteoporosis is increased.

The invention relates to the technical field of gene engineering, in particular to cabbage type rape, a parent species Chinese cabbage thereof, a cabbage TT2 (Transparent Testa 2) gene family and application thereof. The Chinese cabbage TT2 gene family comprises two members of a BrTT2-1 gene and a BrTT2-2 gene; the cabbage TT2 gene family has one member of a BoTT2 gene; the cabbage type rape TT2 gene family comprises three members of a BnTT2-1 gene, a BnTT2-2 gene and a BnTT2-3 gene; and the six brassica TT2 genes have higher homology. The invention also discloses the application of the gene family in plant molecular breeding. After the antisense inhibition of the expression of the cabbage type rape in black-seed cabbage type rape, a transgenic plant generates the character variation of testa color lightening, and the like and has potential for creating transgenic yellow-seed materials.

The invention provides a preparation method for B cell CD 22 extracellular peptide fragment B2285 vaccines. Sequential eight amino acids from the 85th to the 93rd starting at an amino terminal in a mice CD 22 molecule amino acid sequence, specifically in a sequence of Lys- Thr- Glu- Lys- Asp-Pro- Glu- Ser- Glu, are selected. The sequence is similar to the sequence of Lys- Asp- Gly- Lys- Val- Pro-Ser- Glu, having the sequential eight amino acids from the 81st to the 88th starting at the amino terminal in a human CD 22 molecular amino acid sequence, is located at the same functional region in a spatial structure, and has high homology. A prepared vaccine or a drug composite containing B2285 has the functions of inhibiting B cell proliferation, activating and generating antibody per se, canbe applicable to treating immunologic diseases involved or mediated B cells such as dilated cardiomyopathy testing positive in an anti-myocardial antibody, systemic lupus erythematosus with a positive antibody per se, and the like.