47results about How to "Avoid immunogenicity" patented technology

The invention discloses a novel antifreeze polypeptide with a clear structure and excellent performance and a protection method of cryopreserved cells. The core structure of the polypeptide is formed by covalently connecting two functional modules, the N end is a unit with ice crystal binding performance, the C end is a super-hydrophilic unit, and the two functional modules are bridged through a flexible linker. Experimental verification shows that the synthetic polypeptide shows excellent anti-freezing activity, can significantly inhibit nucleation, growth and recrystallization processes of ice crystals, and has particularly outstanding ice recrystallization inhibition activity. The novel antifreeze polypeptide shows excellent biocompatibility, and is suitable for low-temperature cryopreservation of cells sensitive to freezing damage and other living microbial preparations. Results show that the polypeptide can greatly improve the freeze-drying survival rate of cells and the activity of the cells after recovery, is non-toxic to the cells, and shows the potential of replacing a traditional cryoprotectant. Therefore, the method has wide application prospects in food industry, biological medicine, agricultural breeding and low-temperature preservation technologies of biological sample libraries.

The invention belongs to the technical field of in-vitro transcription, and particularly discloses a 5 'UTR element with a protein expression enhancing function, an expression vector and application. The 5 'UTR is selected from any one of an SPP1d element, a TSPAN1u1 element and an SPP1d-TSPAN1u1 series element; the nucleotide sequence of the SPP1d element is as shown in SEQ ID NO: 1, the nucleotide sequence of the TSPAN1u1 element is as shown in SEQ ID NO: 2, and the nucleotide sequence of the SPP1d-TSPAN1u1 tandem element is as shown in SEQ ID NO: 3. The 5 'UTR element can significantly improve translation efficiency, has a wide enhancement effect on functional protein expression, is high in biological safety, and provides a standardized and high-performance translation regulation tool for the fields of efficient recombinant protein production, gene therapy vector optimization, vaccine research and development and the like.