2 results about "Exenatide" patented technology

The present application belongs to the technical field of protein drugs, and relates to a preparation method of an exenatide variant and a polyethylene glycol conjugate thereof. Specifically, Cys 39 The preparation method of Exendin-4 adopts three-step reversed-phase column chromatography for purification, while the preparation method of mPEG-ppMAL-Cys 39 The preparation method of Exendin-4 adopts three-step reversed-phase column chromatography for purification, while the preparation method of mPEG-ppMAL-Cys 39 The preparation method of Exendin-4 adopts three-step reversed-phase column chromatography for purification, while the preparation method of mPEG-ppMAL-Cys When the polypeptide is purified, three-step reversed-phase column chromatography is used, and the acidic substance used in the second step is optimized, so that the purity and yield of the product are obviously improved. When the conjugate is purified, ion exchange chromatography and three-step reversed-phase column chromatography are used in sequence, so that the purity of the product can reach more than 99.5%, and the yield is high. The combined use of the two preparation methods can meet the needs of industrial production.

This invention relates to a sustained-release injectable composition containing exenatide and its preparation method. Using this sustained-release injectable composition, exenatide can be continuously released in vivo for 1 to 2 months with a single injection, thereby reducing the frequency of administration and improving convenience. Furthermore, by encapsulating exenatide in uniformly sized microparticles, a prolonged sustained release of exenatide is achieved, resulting in improved bioavailability compared to existing exenatide injectable formulations.