4 results about "Exenatide" patented technology

Disclosed are methods of improving nutrition in a subject, including an individual who has undergone gastrointestinal surgery, by an exenatide therapy. Exenatide can be administered subcutaneously or intravenously as part of a liquid pharmaceutical formulation. Exenatide therapy may improve body weight and / or nutrition intake / condition in a subject.

The present application belongs to the technical field of protein drugs, and relates to a preparation method of an exenatide variant and a polyethylene glycol conjugate thereof. Specifically, Cys 39 The preparation method of Exendin-4 adopts three-step reversed-phase column chromatography for purification, while the preparation method of mPEG-ppMAL-Cys 39 The preparation method of Exendin-4 adopts three-step reversed-phase column chromatography for purification, while the preparation method of mPEG-ppMAL-Cys 39 The preparation method of Exendin-4 adopts three-step reversed-phase column chromatography for purification, while the preparation method of mPEG-ppMAL-Cys When the polypeptide is purified, three-step reversed-phase column chromatography is used, and the acidic substance used in the second step is optimized, so that the purity and yield of the product are obviously improved. When the conjugate is purified, ion exchange chromatography and three-step reversed-phase column chromatography are used in sequence, so that the purity of the product can reach more than 99.5%, and the yield is high. The combined use of the two preparation methods can meet the needs of industrial production.

The invention belongs to the technical field of medicines, and particularly relates to a polypeptide sustained-release microsphere preparation and a preparation method thereof, the polypeptide sustained-release microsphere preparation comprises the following components: an active component: exenatide or a pharmaceutically acceptable salt thereof; the carrier material is polylactic acid or polylactic acid-glycolic acid copolymer with the molecular weight of 10-50kDa, and the molar ratio of lactic acid to glycolic acid is (50: 50)-(85: 15); surface modification; according to the polypeptide sustained-release microsphere preparation and the preparation method thereof, chemical degradation of exenatide in the preparation process is reduced by optimizing a solvent system and a low-temperature nitrogen protection dissolution process, and meanwhile, the degradation rate of a polymer is optimized by adopting a gradient hardening process, so that sudden release and attenuation in the later stage of release are avoided; the n-heptane, the ethanol and the silicone oil are separated through a multi-stage solvent recovery device, so that the content of the residual solvent accords with the standard, and meanwhile, the consumption of auxiliary materials is reduced through solvent gradient cleaning and silicone oil recovery technologies.

This invention relates to a sustained-release injectable composition containing exenatide and its preparation method. Using this sustained-release injectable composition, exenatide can be continuously released in vivo for 1 to 2 months with a single injection, thereby reducing the frequency of administration and improving convenience. Furthermore, by encapsulating exenatide in uniformly sized microparticles, a prolonged sustained release of exenatide is achieved, resulting in improved bioavailability compared to existing exenatide injectable formulations.