223 results about "Clearance rate" patented technology

This invention relates to transgenically produced human Antithrombin III (tgATIII). The human ATIII produced by the transgenic process of the present invention has a monosaccharide composition which comprises N-acetylgalactosamine (GalNAc) along with fucose, N-acetylglucosamine, galactose, mannose, and N-acetylneuraminic acid/N-glycolyneuraminic acid. The monosaccharide composition differs with that of plasma derived ATIII (phATIII). It has been found that tgATIII has an increased clearance rate when compared to phATIII.

The present invention provides for IgG1 molecules with improved characteristics. In particular, substitution mutations are provided that, in combination, facilitate improved placental transfer, improved serum half-life and improved FcRn binding. Substitution mutations are also provided, that in combination, can be used to block FcRn function and thereby increase the clearance rates of other (endogenous or exogenous) IgGs, block placental transport of IgGs and have increased affinity/reduced pH dependence for FcRn binding.

The invention relates to a compound catalytic material for purifying harmful gas. Deionized water is used as dispersant to compound nano-catalytic material, rare earth compound, p-type semiconductor, antimicrobial, plant extract and anion powder. The compound catalytic material can fast and thoroughly eliminate free formaldehyde, benzene homologue, ammonia and TVOC harmful gas. The compound catalytic material does not require ultraviolet light, has simple use and low cost and does not have toxic side effect and secondary pollution, and the purification effect can last for more than five years without rebound. At the same time, the compound catalytic material prevents bacteria, germina and mildew from invading human body, removes peculiar smell, odor, smoke, leather flavour, paint flavour and the like, increases anion and far infrared ray which are beneficial to human body and improves indoor air environmental quality comprehensively. The experiment detection shows that formaldehyde clearance rate within 24 hours is 91.6 percent; benzene clearance rate is 88.9 percent; ammonia clearance rate is 90.5 percent; TVOC clearance rate is 87.6 percent; sterilization and bacteriostasis rate is 99.99 percent; mould prevention is 0 grade; and acute oral toxicity is tested as actually nontoxic class.

Humanized anti-IL-8 monoclonal antibodies and variants thereof are described for use in diagnostic applications and in the treatment of inflammatory disorders. Also described is a conjugate formed by an antibody fragment covalently attached to a non-proteinaceous polymer, wherein the apparent size of the conjugate is at least about 500 kD. The conjugate exhibits substantially improved half-life, mean residence time, and/or clearance rate in circulation as compared to the underivatized parental antibody fragment.

Provided is a method of treating or preventing age-related macular degeneration (AMD) in a patient subject to, or symptomatic of the disease, wherein the method comprises restoring normal lysosomal pH (pH_L), or acidifying an abnormally elevated pH_L, thus decreasing or preventing a damaging accumulation of lipofuscin or waste products in the retinal pigment epithelium (RPE) cells of the eye of the patient. Further, this method is achieved by elevating cAMP by administering or stimulating receptors coupled to a Gs protein in an amount sufficient to decrease the elevated pH_L or restore acidity of said lysosomes, specifically by administering or stimulating receptors comprising D1-like dopamine receptors by the use of D1-like dopamine receptor agonists. Methods for selecting and quantifying the effectiveness of drugs to restore pH_L and determine outer segment clearance rates is also provided using a high through-put screening protocol.

The invention discloses an antibody coupling drug targeting on EGFR, a preparation method thereof and uses thereof. The antibody-conjugated drug targeting EGFR is named LR004-VC-MMAE consisting of anantibody, a cytotoxic drug and a linker, wherein the antibody drug conjugate has a structure represented by the formula I, wherein mAb is an LR004 monoclonal antibody, n=2-8. The novel antibody-conjugated drug LR004-VC-MMAE can not only target EGFR antigen, but also has strong cytotoxicity to tumor cells. Compared with LR004 itself, it did not affect the affinity, endocytosis and targeting of theantibody, and better retained its biological function. Compared to LR004, the antitumor effect of LR004-VC-MMAE antibody-conjugated drug is significantly improved, and the tumor disappeared. Comparedto LR004, LR004-VC-MMAE antibody-conjugated total antibody showed longer half-life, slower clearance rate, lower concentration of free MMAE in plasma, shorter half-life and faster clearance rate, which is conducive to reduce toxicity.

The invention discloses a citric acid-chitosan-modified anticoagulation polyurethane blood dialysis membrane and a preparation method thereof. The membrane is of a hollow fibrous structure, and the inner surface and outer surface are dense cortical layers, and the middle is a porous supporting layer, so that the blood dialysis membrane is high in permeability and separating property and antibacterial property; the inner diameter is 160 to 250mu m; the membrane thickness is 30 to 50 mu m, and the ultrafiltration coefficient is 7.0 to 60 ml/m<2>.h.mmHg; citric acid-chitosan-modified anticoagulation polyurethane is taken as membrane materials, and in membrane solution, the percentage mass content of the modified anticoagulation polyurethane is 15 to 30%, and the percentage mass content of a solvent is 70 to 85%, and the blood dialysis membrane is prepared by using a nonsolvent induced phase separation method. The preparation process of the dialysis membrane is simple and easy to control, and the prepared membrane has good anticoagulant activity, biocompatibility and antibacterial property, and the clearance rates of urea, beta2-microglobulin and albumin are respectively 55 to 80%, 48 to 60%, and 2.5 to 9%, and the rate of resisting pathogenic escherichia coli is 99%.

The invention discloses high-concentration chemical sewage composite inocula. The high-concentration chemical sewage composite inocula comprise, by weight, 180-220 parts of bacillus cereus, 150-160 parts of bacillus circulans, 75-85 parts of Exiguobacterium acetylicum, 90-120 parts of bacillus coagulans, 150-170 parts of pseudomonas aeruginosa, 20-25 parts of micrococcus luteus, 120-140 parts of thiobacillus thiooxidans, 180-230 parts of rhodococci, 20-30 parts of lactobacillus brevis, 50-65 parts of paracoccus denitrificans, 95-112 parts of alcaligenes faecalis, 110-125 parts of pseudomonas putida, 100-130 parts of enterobacter aerogenes, 280-320 parts of beer yeast and 140-160 parts of lactobacillus fermenti. All strains of the composite inocula reasonably cooperate with each other, produce synergistic effects, do not produce antagonism, have a high survival rate, is suitable for one step addition and fast forms dominant bacterial community. The high-concentration chemical sewage composite inocula have unique treatment effects on high-concentration chemical sewage, have a COD clearance rate of 94.17%, reduce an operation cost and promote qualified discharge.

The invention provides a spectrum-effect relationship-based propolis quality control method. The spectrum-effect relationship-based propolis quality control method can forecast the antioxidant activity of propolis according to a propolis fingerprint. The spectrum-effect relationship-based propolis quality control method utilizes high performance liquid chromatography (HPLC) to build a propolis fingerprint and realizes evaluation of antioxidant activity according to potassium permanganate fading time, a DPPH free radical clearance rate, a phosphomolybdic acid binding rate and a hydroxyl radical clearance rate. Through analysis of a propolis fingerprint chromatograph and spectral characteristics, the spectrum-effect relationship-based propolis quality control method determines 25 characteristic peaks and 5 characteristic peak groups. Through fitting of a polynomial, the spectrum-effect relationship-based propolis quality control method builds a mathematical model for forecasting the antioxidant activity of propolis according to a propolis fingerprint. According to the characteristic peaks and the characteristic peak groups, only through the mathematical model, the spectrum-effect relationship-based propolis quality control method can directly determine strength of the antioxidant activity of a propolis drug without an anti-oxidation test thereby determining quality. The spectrum-effect relationship-based propolis quality control method has the advantages of simple processes, strong objectivity and more characteristic peaks and realizes perfect and scientific propolis quality control.

The invention discloses a carbon quantum dot visible light photocatalyst coating and a preparation method thereof. The coating comprises the following raw materials in parts by weight: 1-100 parts of carbon quantum dot aqueous solution, 5-50 parts of polymer emulsion, 0-50 parts of titanium dioxide, 2-20 parts of assistant and 10-50 parts of deionized water. The invention further discloses the preparation method of the coating. The prepared carbon quantum dot photocatalyst coating can efficiently absorb and utilize visible light (400-800nm) in sunlight; under irradiation of the visible light, singlet oxygen can be efficiently generated; harmful gas in an environment can be comprehensively cleared (a clearance rate is above 90%); harmful microorganisms can be efficiently killed (an antibacterial rate is above 95%); the defect of easy conglobation of nano particles is overcome; the storage stability is improved; and the photocatalyst coating is good in film forming property, has scrubbing resistance, and can be used for a long time.

The invention relates to a peony seed meal polypeptide with antioxidant activity as well as a preparation method and application thereof. The method comprises the following steps that neutral proteaseand alkaline protease are subjected to synergistic multi-step enzymolysis, after enzymolysis is conducted for 48 h, centrifugal filtration is conducted, supernate is taken, then the supernate, namelyenzymolysis liquid, is subjected to an antioxidant test, and the enzymolysis liquid is subjected to freeze drying to obtain the peony seed meal polypeptide with the antioxidant activity. The method is simple to operate, treatment conditions are mild, after synergistic multi-step enzymolysis of the neutral protease and the alkaline protease, the purity of a peony seed polypeptide product is high,the molecular weight is even, through TricineSDSPAGE electrophoresis detection, the molecular weight of the peony seed polypeptide product is 5.8-6.2 KDa, and the purity is 75% or above; the antioxidant activity of the peony seed meal polypeptide in the molecular weight range is very outstanding, especially, the capability of the polypeptide of clearing ABTS<+> free radicals is very significant, and when the concentration of the polypeptide is 1 mg/mL, the clearance rate is up to 82%.

The present invention discloses a BiOCl/montmorillonite composite photocatalytic material and a preparation method and an application thereof. The preparation method comprises the following steps of: during stirring, adding a 100 ml 3.0 wt% Bi(NO3)3 solution dropwise to a 100ml 1.0-5.0 wt% sodium-based montmorillonite suspension; after ultrasonic processing is performed, adjusting PH to 2; performing stirring for reaction for 8-16 h; centrifuging the mixed suspension; performing precipitation; washing the precipitate; drying and grinding the precipitate; and sieving the ground precipitate through a 200-mesh sieve to obtain the BiOCl/montmorillonite composite photocatalytic material. Under UV irradiation, the effect of adsorption and photocatalytic degradation is very significant for a cationic dye rhodamine B and an anionic dye orange G, and by using 0.15 g/L of the BiOCl/montmorillonite composite photocatalytic material, both the clearance rates of 40 mg/L of RhB and OG within a relatively short period, 1 h, are close to 100%.

The invention discloses an adsorbent used for processing domestic sewage. The adsorbent comprises the following raw materials in parts by weight: 70-80 parts of wooden activated carbon, 20-30 parts of active alumina, 30-35 parts of walnut peels carbon powder, 10-15 parts of bamboo charcoal, 20-25 parts of bentonite, 10-15 parts of sepiolite, 15-20 parts of attapulgite, 5-10 parts of silica, 5-10 parts of peanut shell, 3-5 parts of sodium hydroxide and 2-5 parts of calcium oxide. The adsorbent has strong adsorption capability for an organic matter in the sewage, has high clearance rate of COD, BOD and total phosphorus, can remove peculiar smell in the sewage, cannot generate secondary pollution, can be regenerated, and has advantage of low cost.

A gradation method of a small-grain-size skeleton dense type asphalt mixture comprises the following steps: 1, determining boundary sieve holes of a main skeleton and filling aggregates; 2, according to a gradation filling theory, obtaining the composition mass ratio of each grade of filling aggregates; 3, determining the optimum mass ratio of each grade of filling aggregates; 4, adopting a grey correlation entropy analysis method to determine key sieve holes which influence the skeleton properties of the asphalt mixture according to the influence of each grade of filling aggregates on the clearance rate and the maximum bearing capability of the main skeleton; 5, obtaining the gradation optimal range of the main skeleton of the asphalt mixture; 6, determining the mass ratio of each grade of filling aggregates; 7, determining key sieve holes that the filling aggregates influence the performance of the small-grain-size asphalt mixture; 8, obtaining the gradation reference range of filler in the asphalt mixture; and 9, determining the ratio of the main skeleton to the filling aggregates through a volumetric method so as to obtain the gradation range of the small-grain-size skeleton dense type asphalt mixture. The gradation method enables the skeleton and the dense state of the mixture to simultaneously reach the optimum and meets the requirements of roads and anti-slip performance.

The invention relates to a method for settling, filtering and removing carbon nanotubes by using calcium ions, is suitable for treating a water body polluted by the carbon nanotubes and belongs to the field of nanotechnology and environmental protection technology. The method comprises a step of: agglomerating carbon nanotubes by adding calcium chloride into carbon nanotube solution so as to remove the carbon nanotubes in the water body by a filtering method. When the calcium ions have a concentration of 0.001mol/L, the clearance rate of the carbon nanotubes is higher than 99 percent; a residual amount is less than 5 mu g/ml; and water solution is colourless and transparent. Under the conditions of different acid and alkaline degrees and ion strengths, the method also can effectively eliminate the carbon nanotubes. Under the presence of a surface active agent and a natural organic matter, the same excellent elimination effect also can be achieved by improving the additive amount of the calcium chloride moderately. The concentration of the calcium ions used for elimination is lower, and purified water is moderately hard water and can be discharged directly without softening treatment.

The invention discloses a dual-energy under-sampling substance identification system and a dual-energy under-sampling substance identification method based on linear track scanning. The method comprises the following steps of: performing computerized tomography (CT) reconstruction on a detected object by using low-energy projection data obtained by a whole layer of low-energy detector array in the linear track scanning process, performing region segmentation on a tomographic image according to an attenuation coefficient by a regional image segmentation method in a CT reconstructed image, and labeling; and meanwhile, realizing dual-energy substance identification imaging by using projection data obtained by a few high-energy detectors arranged behind partial low-energy detectors. A linear track is utilized, and the rotation is not required, so the mechanical design is simple, and the inspection clearance rate is high; and only a few dual-energy probes are used, so the cost of the system is low.

The invention relates to a method for obtaining active polypeptide by carrying out multi-strain compound solid state fermentation on common rapeseed meal. The common rapeseed meal is taken as raw material, plant lactic acid bacteria, bacillus subtilis and aspergillus oryzae are screened for carrying out mixed bacterium solid state fermentation, and concrete fermentation steps are as follows: inoculating in the ratio of plant lactic acid bacteria to bacillus subtilis to aspergillus oryzae being (1-5): (1-3): (1-3), wherein concentration of inoculated bacterium suspension is 108/mL, after inoculation, fermenting in a fermentation bin, and drying at the temperature of 55 DEG C, thus obtaining the active polypeptide. By adopting the method disclosed by the invention, after fermentation, crude protein content is improved by 10%, polypeptide content is increased by more than 6%, clearance rate of the polypeptide to hydroxyl radical is improved by 14 times, the clearance rate of the polypeptide to superoxide anion is improved by 17 times, and the clearance rate of the polypeptide to DPPH (1,1-diphenyl-2-picrylhydrazyl radical) is improved by 12 times.

The invention discloses antibody nanoparticles coated by natural red cell membranes for antibody drug delivery and a preparation method of the antibody nanoparticles. The red cell membranes in the invention are extracted from experimental rats, most of the contents and unrelated proteins are removed after the red cell membranes are treated, and protein nanoparticles formed by antibodies are coated with the obtained red cell membranes. The method is suitable for coating nanoparticles formed by different antibodies through the red cell membranes, can improve the stabilities and the in-vivo circulation times of free antibodies, and further reduces the body immunity clearance rate.

The invention discloses an application of Chinese angelica polypeptide with effects of resisting oxidization and delaying ageing in preparation of food, particularly of health food, and further discloses a preparation method of the Chinese angelica polypeptide. The preparation method is simple and easy to implement, safe and non-toxic, stable and efficient, and is suitable for industrial production. In vitro, the Chinese angelica polypeptide has a characteristic of a certain hydroxyl radical clearance rate, which means that the Chinese angelica polypeptide has capacity of clearing active oxygen; at an integral animal level, an oxidative stress model and an ageing-associated model are used to perform a test, the test result discloses that the survival time of caenorhabditis elegans under an oxidative stress condition can be remarkably prolonged, the lipofuscin level in the caenorhabditis elegans is reduced, average lifetime of the caenorhabditis elegans is prolonged; under the oxidative stress condition and the ageing condition, the Chinese angelica polypeptide can be used for realizing remarkable anti-oxidization and ageing delaying effects by lowering the reactive oxide species (ROS) in vivo and lowering the content of lipid peroxide.

The invention discloses a preparation method of a natural antioxidant tricholoma lobayense heim polysaccharide, comprising the following steps of: drying tricholoma lobayense heim until the tricholoma lobayense heim has a constant weight and then smashing and sieving; then digesting obtained powder with hot water, rotatably evaporating and concentrating an extracting solution, and taking liquid supernatant after centrifuging; and finally, concentrating the liquid supernatant, taking sediments after ethanol precipitation and centrifuging, and freezing and drying, so as to obtain the tricholoma lobayense heim polysaccharide. The tricholoma lobayense heim polysaccharide is obtained by extracting is a natural product with high antioxidant activity and can be used for obviously cleaning.O2<-> generated by a pyrogallic acid self-oxidation system, particularly a component of TL-3. When the concentration is 500 microgram/mL, the clearance rate can reach 75.6%, which is only 15.4% lower than Vc. Therefore, the tricholoma lobayense heim polysaccharide can be used as a natural non-toxic antioxidant in fields such as foods, medicines, cosmetics and the like.