157 results about "ESCHERICHIA COLI ANTIGEN" patented technology

The present invention relates to pharmaceutical compositions suitable for the treatment of chronic diseases associated with the presence of abnormal or an abnormal distribution of microflora in the gastrointestinal tract of a mammalian host, which compositions comprise viable non-pathogenic or attenuated pathogenic Clostridia. The compositions further comprise one or more additional viable non-pathogenic or attenuated pathogenic microorganisms selected from the group consisting of Bacteroides, Eubacteria, Fusobacteria, Propionibacteria, Lactobacilli, anaerobic cocci, Ruminococcus, E.Coli, Gemmiger, Desullomonas, Peptostreptococcus, and fungi. The present invention also provides pharmaceutical compositions suitable for the treatment of the same chronic diseases comprising viable non-pathogenic or attenuated pathogenic Escherichia coli, at least one strain of viable non-pathogenic or attenuated pathoenic Bacteroides and at least one strain of viable non-pathogenic or attenuated pathogenic microorganism.

The invention relates to biological compositions that can be used to prevent the spread of pathogenic Escherichia coli infections in the environment. The biological compositions of the invention comprise a plurality of live yeast cells which are capable of limiting or suppressing the growth of pathogenic E. coli O157:H7. The biological compositions can be used for reducing the incidence of infections of animals by pathogenic O157:H7 strains of E. coli in farm operations. The invention also relates to methods for manufacturing the biological compositions, and methods of using the biological compositions.

The invention relates to a pathogenic microorganism DNA detection chip and the chip comprises a carrier and a nucleic acid probe on the carrier. The nucleic acid probe is provided with a target detection probe used for detecting target gene of pathogenic microorganism. The pathogenic microorganism comprises but not limits to vibrio cholerae, pathogenic escherichia coli, campylobacter jejuni, Yersinia enterocolitica, parahemolytic vibrio, salmonella, and shigella and Listeria monocytogenes. The invention also relates to a preparation method of the pathogenic microorganism DNA detection chip and provides applications of the pathogenic microorganism DNA detection chip in detection of pathogenic microorganism. Also a pathogenic microorganism DNA detection chip kit is provided.

The invention discloses a feed compound enzyme-containing dedicated enzyme for growing pigs and a preparation method of the feed compound enzyme-containing dedicated enzyme, and belongs to the technical field of preparation of enzyme preparations. The dedicated enzyme for the growing pigs is prepared by scientifically compounding aspergillus niger cultures, acidic xylanase, phytase, beta-dextranase, cellulase, amylase, acid proteinase, Chinese herb extracts, a protective agent and an activating agent, wherein the aspergillus niger cells in the aspergillus niger cultures can inhibit the growth of pathogenic escherichia coli, inhibit the growth and breeding of aspergillus flavus, disintegrate the aspergillus flavus, promote the growth of animals, adjust gastrointestinal tract flora to be balanced, and enhance whole immunity during the processes of growth and fermentation, in addition, the fermentation liquid crude enzyme liquid of the aspergillus niger cultures contains different enzyme activity such as protease activity, mannase activity, alpha-galactase activity and pectinase activity, and the dedicated enzyme is specially suitable for being added in the feed for the growing pigs.

The invention belongs to the technical field of biology detection and relates to an escherichia coli O1, O2, O18 and O78 serotype detection kit and a detection method thereof. Forward primers of a primer group of the kit are highly conservative escherichia coli O antigen combined relative gene sequences, and backward primers are four serotype specific primers designed on the basis of four O1, O2, O18 and O78 serotype escherichia coli O antigen synthesis relative genes. The detection kit containing the primer group and the detection method of the detection kit have the advantages of being fast, sensitive, specific, low in cost, easy to operate and capable of well overcoming the shortcomings in the current escherichia coli O1, O2, O18 and O78 serotype detection method, can meet the requirements for the current escherichia coli O1, O2, O18 and O78 serotype detection, can be popularized and used easily in a wide range and have wide market prospect and large economic benefit.

The invention discloses a citric acid-chitosan-modified anticoagulation polyurethane blood dialysis membrane and a preparation method thereof. The membrane is of a hollow fibrous structure, and the inner surface and outer surface are dense cortical layers, and the middle is a porous supporting layer, so that the blood dialysis membrane is high in permeability and separating property and antibacterial property; the inner diameter is 160 to 250mu m; the membrane thickness is 30 to 50 mu m, and the ultrafiltration coefficient is 7.0 to 60 ml / m<2>.h.mmHg; citric acid-chitosan-modified anticoagulation polyurethane is taken as membrane materials, and in membrane solution, the percentage mass content of the modified anticoagulation polyurethane is 15 to 30%, and the percentage mass content of a solvent is 70 to 85%, and the blood dialysis membrane is prepared by using a nonsolvent induced phase separation method. The preparation process of the dialysis membrane is simple and easy to control, and the prepared membrane has good anticoagulant activity, biocompatibility and antibacterial property, and the clearance rates of urea, beta2-microglobulin and albumin are respectively 55 to 80%, 48 to 60%, and 2.5 to 9%, and the rate of resisting pathogenic escherichia coli is 99%.

The invention relates to a lactobacillus pentosus strain and a fermentation product of the same. The preservation number of the lactobacillus pentosus LPEM818 in the invention is CGMCC No. 4583 in the China General Microbiological Culture Collection Center. The fermentation product of the lactobacillus pentosus in the invention can inhibit common pathogenic escherichia coli, pseudomonas aeruginosa and staphylococcus aureus in food, and particularly has a strong inhibiting effect on listeria monocytogenes which can cause serious food poisoning. In addition, the metabolite of the strain also can inhibit the growth of aspergillus flavus and cotton and tomato blight pathogens. Consequently, the lactobacillus pentosus strain and the fermentation product thereof play an active role in the fields of food preservation and plant protection.

The invention discloses escherichia coli bacteriophage vB_EcoM_swi3 of which the preservation number is CCTCC M 2019467. The bacteriophage belongs to myoviridae, and is provided with a polyhedral headand a telescopic tail structure, wherein the diameter of the head is about 80nm, and the length of the tail is about 120nm. Bright plaque can be formed on a solid culture medium, an aureole does notexist on the periphery of the plaque, the edge is clear and regular, and the diameter is about 1-1.5mm. The bacteriophage has favorable splitting effects on pig-derived escherichia coli and chicken-derived escherichia coli, especially pig-derived pathogenic escherichia coli and chicken-derived pathogenic escherichia coli, and has quite great application prospects in a plurality of respects of medicinal preparations for preventing and treating pig-derived colibacillosis and chicken-derived colibacillosis, feed additives and the like.

The invention relates to a method for preparing lactoferrin antibacterial peptide through an enzyme method which belongs to the technical field for preparing the lactoferrin antibacterial peptide. The method of the invention comprises: taking lactoferrin as raw materials, adopting pig pepsin to hydrolyze the lactoferrin under certain condition, and finally obtaining lactoferrin antibacterial peptide mixture with stronger antibacterial activity. The method has simple process and strong practicability, the lactoferrin antibacterial peptide which is obtained has stronger antibacterial property and is broad-spectrum antibacterial peptide, gram-negative bacteria and gram-positive bacteria which contain pathogenic escherichia coli can be inhibited, eukaryotic cells are not affected, and the lactoferrin antibacterial peptide is functional antibacterial peptide and has better application prospect. The method for preparing the lactoferrin antibacterial peptide has important significance for promoting dairy products deep processing and colostrum resources comprehensive utilization, increasing health level of our people especially infants, and researching and developing functional food additives.

The invention belongs to the technical field of veterinary biology, and particularly relates to a colon bacillus outer membrane protein monoclonal antibody and a preparation method and an application thereof. The antibody is prepared by taking cloned, expressed and purified taking avian pathogenic escherichia coli 78 (APECO78) outer membrane protein as an antigen immune BALB / c mouse with a hybrid tumor technology. The antibody has high specificity and high sensitivity, and an experimental basis is laid for future rapid and specific detection of colon bacillus infection with an immunology method. Meanwhile, as proved by an experiment, the monoclonal antibody disclosed by the invention has high neutralizing activity and protecting efficiency on colon bacillus infection of the same type, and can be used for preventing colibacillosis.

The invention discloses application of recombinant escherichia coli with overexpression of a fimH gene in the fermentation production of amino acid. According to the invention, by drawing lessons fromthe medical experience that compared with free cells, cells of a biological membrane formed by pathogenic escherichia coli are more adaptable to extreme environments, the formation of an escherichiacoli biological membrane can be regulated and controlled by the fimH gene regulated and controlled by a known fim operon, the change of an amino acid metabolic pathway after overexpression of the fimHgene is discovered through transcriptome analysis, groundwork is laid for screening recombination strains of other high-yield amino acid organic acid in a industrialization process in a later period,and the goal of increasing the yield, the fermentation rate and the sugar conversion rate is finally achieved.

The invention provides a multiplex PCR (Polymerase Chain Reaction) primer group for avian pathogenic escherichia coli, pasteurella multocida, proteus mirabilis, pseudomonas aeruginosa, salmonella andstaphylococcus aureus, a multiplex PCR detection kit comprising the primer group, and a multiplex PCR detection method using the primer group. The primer group comprises primer pairs PhoA-F and PhoA-Rfor specifically amplifying a gene phoA of the avian escherichia coli, primer pairs KMT-F and KMT-R for specifically amplifying a gene KMT1 of the pasteurella multocida, primer pairs AtpD-F and AtpD-R for specifically amplifying a gene AtpD of the proteus mirabilis, primer pairs PETA-F and PETA-R for specifically amplifying a gene PETA of the pseudomonas aeruginosa, primer pairs InvA-F and InvA-Rfor specifically amplifying a gene InvA of the salmonella, and prier pairs NuC-F and NuC-R for specifically amplifying a gene nuc of the staphylococcus aureus.

The invention belongs to the field of biotechnology, and provides an avian pathogenic escherichia coli strain, which is an avian pathogenic escherichia coli luxS and aroA double-gene-deleted strain with the preservation numbr of CGMCC10601. The avian pathogenic escherichia coli strain provided by the invention can be used for preparing avian pathogenic escherichia coli inactivation and attenuated vaccine and bacterial ghost vaccine, and preventing avian pathogenic escherichia coli infection.

The invention discloses an escherichia coli bacteriophage vBE_coM_swi3 with a preservation number of CCTCC NO: M2019467. The bacteriophage belongs to myoviridae and has a polyhedral head and a telescopic tail structure, the diameter of the head is about 80 nm, and the length of the tail is about 120 nm; transparent empty spots can be formed on a solid culture medium, no halo ring exists around thesolid culture medium, the edge is clear and regular, and the diameter is about 1-1.5 mm; the bacteriophage has a good cracking effect on swine-derived and chicken-derived escherichia coli in a breeding environment, particularly on swine-derived and chicken-derived pathogenic escherichia coli, and has very wide application prospects in multiple aspects of pharmaceutical preparations, feed additives and the like for preventing and treating swine colibacillosis and chicken colibacillosis.

The invention provides a method for wheat bran complex fermentation by utilizing a bacterium enzyme and belongs to the technical field of biological fermentation. According to the method, the following constituents are added into each gram of wheat bran: 2*109 endomycopsis yeast, 4*109 bacillus subtilis, 5*109 lactic acid bacteria, 10 U Alpha-amylase and 10 U phytase; the material-to-water ratio is regulated between 1:0.7 and 1:1; sealing fermentation is carried out at 30-50 DEG C for 48 h. The method is simple in process and low in cost, and has the advantages that solid fermentation is adopted; the final product needs not to be dried so as to avoid microorganism disruption; neither phytic acid nor mycotoxin is detected after fermentation; meanwhile, the fermented wheat bran is rich in a variety of probiotics and metabolites thereof, so that the growth and reproduction of harmful germs such as pathogenic escherichia coli and salmonella can be effectively restrained, and the animal immunity can be improved.

The invention discloses a special enzyme containing a feed composite enzyme for piglets and a preparation method thereof, belonging to the technical field of preparation of enzyme preparations. The special enzyme for piglets is prepared by scientifically compounding Aspergillus niger culture, acidic xylanase, cellulase, beta-glucanase, amylase, phytase, Chinese herbal medicine extract, a protective agent and an activator, wherein Aspergillus niger body in the Aspergillus niger culture can inhibit the growth of pathogenic escherichia coli in processes of growth and fermentation, inhibit the growth and reproduction of Aspergillus niger, decompose aflatoxin, promote animal growth, regulate flora balance in gastrointestinal tract, and improve overall immunity; the crude enzyme liquid of fermentation liquor of the Aspergillus niger culture contains activity of multiple enzymes such as protease, mannase, alpha-galactase and pectinase, thus being especially suitable for being added into feed for piglets.

The invention provides an upconversion-immunochromatography test strip for detecting Escherichia coli O157:H7, and a detection method thereof. The test strip comprises a sample pad, a conjugate pad, an analysis membrane and an absorbent pad, the sample pad and the conjugate pad are glass cellulose membranes, the analysis membrane is a nitrocellulose membrane, the absorbent pad is a cellulose membrane, the analysis membrane is provided with a detection line and a quality control line, the sample pad, the conjugate pad and the absorbent pad are sequentially pasted on a PVC plate, the conjugate pad is immobilized with an upconversion luminescence nanoparticle-Escherichia coli O157:H7 monoclonal antibody IgG conjugate, the detection line on the analysis membrane is immobilized with an Escherichia coli O157:H7 antigen, and the quality control line is immobilized with MAbF1. The above upconversion nanomaterial-based immunofluorescence test strip has the advantages of low sensitivity, wide detection range, stable signal, no photobleaching, low background, sensitive signal, and accurate and reliable result.

The invention relates to myoviridae bacteriophage Esc-COP-7 (commissioned number: KCTC 13130BP) separated from the nature. The Esc-COP-7 is characterized by carrying a genome which has specific Escherichia coli killing capacity and is marked with a serial number 1. The invention also relates to a method for preventing and treating pathogenic Escherichia coli infection by use of composition containing the bacteriophage as an effective component.

The invention discloses Bacillus pumilus JY2 with the preservation number of CGMCC NO. 9150. The Bacillus pumilus JY2 is sampled from a winter wheat field (N 72 degrees 50 minutes 70.01 seconds E40 degrees 26 minutes 51.73 seconds) in an Osh race course of Kyrgyz Republic and is subjected to separating, screening and physiological and biochemical identification. The Bacillus pumilus JY2 with the preservation number of CGMCC NO. 9150, provided by the invention, has a good application effect on inhibiting pathogenic escherichia coli and salmonella, has a remarkable technical effect on applications in feeding microbioecologics and has broad application prospect in feed additive industries.

The invention provides a pediococcus pentosaceus NHB-PpA9601. The pediococcus pentosaceus NHB-PpA9601 is separated from intestinal tracts of white feather broilers, and is preserved in China General Microbiological Culture Collection Center (CGMCC for short, the address is Institute of Microbiology, Chinese Academy of Sciences, 3 #, 1 # yard, West Beichen Road, Chaoyang District, Beijing, the postal code is 100101), the preservation number is CGMCC NO.22646, and the preservation date is May 31, 2021. The pediococcus pentosaceus NHB-PpA9601 has the advantages that the obvious probiotic property is realized, the growth and reproduction of pathogenic bacteria such as intestinal pathogenic escherichia coli, staphylococcus aureus, salmonella suis, salmonella choleraesuis, salmonella gallinarum, salmonella pullorum, salmonella typhimurium, salmonella, shigella, bacillus perfringens, proteus penneri, aeromonas hydrophila and vibrio parahaemolyticus can be effectively inhibited; the pediococcus pentosaceus NHB-PpA9601 can be used for producing a wet-based fermented feed, and can be used for effectively improving intestinal health and increasing economic benefits.

The invention provides a method, a gene chip and a detection reagent kit for detecting common cow pathogenic escherichia coli. The gene chip comprises a solid phase carrier and an oligonucleotide probe fixed on the solid phase carrier, wherein the oligonucleotide probe comprises DNA (Deoxyribonucleic Acid) fragments selected from a glycosyl transferase gene and an oligose unit processing enzyme gene on a main escherichia coli serotype O-antigen gene cluster relative to cow septicemia and diarrhea. The gene chip and a designed primer are utilized for detecting and can be prepared into the reagent kit. The products and the method of the invention are utilized to reach the purpose for detecting the serotype of the common cow pathogenic escherichia coli and have the advantages of simple and convenient operation, high throughput, high accuracy, strong repeatability and important significance on the research and the diagnosis of epidemiology.

The invention provides a traditional Chinese medicine composition for treating porcine pathogenic Escherichia coli diseases. The raw material medicines comprise common andrographis herb, scutellaria baicalensis, white paeony root, cacumen biotae, hempleaf negundo chastetree leaf, the root of Chinese pulsatilla, codonopsis pilosula, radix bupleuri, madder, smoked plum, centipeda minima, hawthorn, pericarpium citri reticulatae, rhizoma polygonati, rhizoma kaempferiae, honeysuckle stem, rhizoma galangae, adenophora tetraphylla, gorgon fruit, corydalis tuber, folium ginkgo, fringed pink and semen raphani. The traditional Chinese medicine composition provided in the invention has the advantages of bacteria resistance, virus resistance, small side effects, zero residue and no drug resistance, is a good substitute of antibiotics and has a wide development prospect in the aspect of treating the porcine pathogenic Escherichia coli diseases.

The invention relates to the field of microecological feed additives, and particularly relates to preparation and application of a composite microecological feed additive. The composite microecological feed additive provided by the invention is prepared from screened active compound probiotics. Diarrhea caused by domestic animal bacterial infections can be prevented and treated. Two probiotics used by the composite microecological feed additive have the abilities of inhibiting infection of common gastrointestinal pathogens of animals, including shigella dysenteriae, pathogenic escherichia coli and salmonella enteritidis, and also can reduce the toxicity caused by dysentery bacillus infection Caco-2 cells and inhibit adhesion of the shigella dysenteriae to the Caco-2 cells. Finally, the obvious effects of stimulating the animal immunity, improving the gastrointestinal digestion abilities of animals, and promoting animal growth are achieved. A production technology of a strain adopts a liquid fermental cultivation technology and a low-temperature freeze drying technology, so that the obtained inoculant is high in survival rate and purity. The composite microecological feed additive is a green environment-friendly feed additive.

The invention belongs to the veterinary biotechnical field, in particular to a riemerella anatipestifer-escherichia coli outer membrane protein bivalent vaccine and a preparation method thereof. The riemerella anatipestifer-escherichia coli outer membrane protein bivalent vaccine is prepared by emulsifying an antigen and oil adjuvant according to the volume ratio of 1:1-1:5, wherein the antigen is a riemerella anatipestifer RA1 outer membrane protein and / or a riemerella anatipestifer RA2 outer membrane protein and an avian pathogenic escherichia coli 078 outer membrane protein. As proved by an animal experiment, a vaccine immune animal prepared by adopting the method has high vaccine safety and a remarkable immune effect, a high-titer antibody can be generated inside the animal, and the attack of the same type of riemerella anatipestifer and a plurality of serum escherichia coli can be resisted.

The invention discloses an RPA-LFD primer pair and probe for detecting pathogenic escherichia coli O157:H7 and application thereof. As for the sequences of the primer pair and the probe, the nucleotide sequence of an upstream primer EOF4 is SEQ ID NO.6; the nucleotide sequence of a downstream primer EOR3 is SEQ ID NO.7; the nucleotide sequence of the probe EOProb is SEQ ID NO.9. By means of a method, the defects of existing detection technologies are overcome through safe, specific, rapid, sensitive and simple on-site detection. It is proved through experiments that the minimum reaction volumeof an RPA-LFD technology is 10 microliters, the optimum reaction temperature is 40 DEG C, the shortest detection time is 15 min, the reaction cost is greatly saved, and the time required for detection is shortened. The optimum primers screened by the RPA reaction are high in specificity and sensitivity, and the sensitivity of RPA-LFD is 100 times that of PCR.

The invention belongs to the technical field of biology and relates to a preparation method and application of a novel antimicrobial peptide Misgurin mutant. The technical scheme is as follows: introducing glutamine at the C tail end of the amino acid sequence of natural Misgurin so as to design a novel Misgurin mutant; selecting Pichia yeast biased codons so as to artificially synthesize a complete gene of the novel Misgurin mutant; constructing a Pichia yeast secretion type expression vector pPICZaA-M; transforming a host cell SMD1168 by a vector electroporation method; selecting to obtain high-efficiency expression strains; and under proper culture conditions, ensuring 100 microgram of recombinant novel Misgurin mutant is contained in per milliliter of expression supernatant. The obtained product has a good nematocidal activity on staphylococcus aureus, pig salmonella and pig pathogenic Escherichia coli K88, K99 and 987P, and has an obvious effect on prevention and treatment of clinical bacterial diseases.

The present invention provides a polypeptide, called EspA, which is secreted by pathogenic E. coli, such as the enteropathogenic (SPEC) and enterohemorrhagic (EHEC) E. coli. The invention also provides isolated nucleic acid sequences encoding EspA polypeptide, EspA peptides, a recombinant method for producing recombinant EspA, antibodies which bind to EspA, and a kit for the detection of EspA-producing E. coli.