10 results about "Dna detection" patented technology

The application discloses a method for adenovirus whole genome sequencing, a primer group and a kit thereof, and belongs to the technical field of DNA detection.The method for adenovirus whole genome sequencing comprises the following steps: S1, first round amplification is carried out by using first round amplification primers with sequences shown in SEQ ID NO.1 to SEQ ID NO.93 in Table 1; and S2, second round amplification is carried out by using second round amplification primers with sequences shown in SEQ ID NO.94 to SEQ ID NO.285 in Table 2.The average length of data sequenced by the method is 0.8 kb, compared with short read length of a second-generation platform, the uniformity of adenovirus coverage can be greatly improved, meanwhile, the adenovirus types are comprehensively covered, and the method can be widely promoted.

The application discloses a pathogen DNA detection optimization method based on deep learning, and belongs to the technical field of bioinformatics and deep learning. The first electric signal data and the first DNA sequence data of nanopore sequencing are acquired; the first electric signal feature vector is output through an electric signal feature enhancement extraction network; the first sequence feature vector is output through a sequence feature coding network; the first fusion feature vector is obtained through element-by-element weighted summation of a feature layer fusion network; the first pathogen existence probability value is output through a first pathogen classification network and compared with the first filtering threshold value 0.15 for rapid screening; the second fusion feature vector is obtained through a time sequence context coding network and a cross attention fusion network for the sample; the second pathogen existence probability value is output through a second pathogen classification network; and the detection is completed according to the application scene parameter selected 0.35, 0.50 or 0.65 determination threshold value. The application realizes the cooperative utilization of electric signal and sequence features and the dynamic adjustment of the detection strategy.

This invention discloses a DNA detection method based on LAMP and immunochromatography, belonging to the field of biomedical detection technology, including the following steps: S1, LAMP amplification and product labeling: adding sample dilution buffer to a LAMP reaction tube, and using FITC-labeled specific primers to perform LAMP isothermal amplification of the target DNA in the sample; S2, immunochromatographic reagent detection: using a double-antibody sandwich immunochromatography method, the FITC-labeled LAMP product is visualized and detected by colloidal gold; S3, result determination. This DNA detection method based on LAMP and immunochromatography can achieve efficient amplification of target DNA under isothermal conditions using LAMP primers, and label the amplified product by pre-incorporating FITC-labeled specific primers. Subsequently, the signal is visualized and read out using a double-antibody sandwich immunochromatography method with colloidal gold, thereby achieving highly sensitive, highly specific, and rapid visual detection of target DNA.

This invention discloses an optimized method for pathogen DNA detection based on deep learning, belonging to the interdisciplinary field of bioinformatics and deep learning. The invention acquires first electrical signal data and first DNA sequence data from nanopore sequencing; outputs a first electrical signal feature vector through an electrical signal feature enhancement extraction network; outputs a first sequence feature vector through a sequence feature encoding network; obtains a first fused feature vector by element-wise weighted summation through a feature layer fusion network; outputs the probability value of the presence of a first pathogen through a first pathogen classification network and compares it with a first filtering threshold of 0.15 for rapid screening; obtains a second fused feature vector for the samples using a temporal context encoding network and a cross-attention fusion network; outputs the probability value of the presence of a second pathogen through a second pathogen classification network; and completes the detection by selecting a judgment threshold of 0.35, 0.50, or 0.65 according to the application scenario parameters. This invention achieves the synergistic utilization of electrical signals and sequence features and dynamic adjustment of the detection strategy.

The application discloses a whole-genome SNP molecular marker combination related to the apraocha of sea cucumber against vibrio splendidus and application thereof, wherein the SNP molecular marker combination comprises 35 SNP sites, and in disease-resistant breeding, individuals with the 35 SNP site dominant genotypes can be screened to accelerate the breeding process of the disease-resistant traits. The molecular marker provided by the application has high biological correlation and prediction accuracy in breeding, and can be screened through DNA detection without relying on the phenotype of individual infection and disease, so that the generation interval is greatly shortened, the breeding efficiency is improved, the molecular marker assisted selection can overcome environmental interference, the genotypes are directly selected, the selection strength is high, the accuracy is high, and the genetic progress of the disease-resistant traits is accelerated.

The present invention relates to a primer, a DNA detection method, and a DNA detection kit for use in nucleic acid amplification, and particularly in asymmetric nucleic acid amplification. More specifically, this invention relates to a primer for introducing a mutation into a test nucleic acid and amplifying the test nucleic acid, the primer contains, in its sequence, a mutation to be introduced into the test nucleic acid, so as to increase a proportion of single-strand forming bases in a probe binding region of the nucleic acid amplified from the test nucleic acid, at a temperature of the binding of the nucleic acid amplified from the test nucleic acid with the probe; and, a DNA detection method and a DNA detection kit with use of such primer.

This invention discloses a system and method for monitoring gastrointestinal function recovery after gastrectomy, relating to the field of gastrointestinal nursing technology. The method involves acquiring samples from target patients after gastrectomy, obtaining the microbial distribution in the samples, and gathering preoperative microbial data and gastrectomy surgical characteristics of the target patients. Based on the baseline status and surgical characteristics of the target patients, population information is obtained. An antibiotic impact model is trained. The microbial distribution in the samples is optimized based on the antibiotic impact model and the microbial distribution in the samples, and the optimized microbial distribution is provided to medical staff as a reference. The influence of intestinal transit time on antibiotic absorption and the impact of antibiotics on the microbial community are analyzed, establishing an antibiotic impact model and optimizing DNA detection information. This reduces interference with DNA detection caused by the use of antimicrobial agents after gastrectomy without RNA detection, improving the reference value and usability of the data.

This invention pertains in general to probes for detecting and / or quantifying double- stranded DNA. The invention particularly relates to luminescence based probes, and methods of using such probes to detect or quantify double-stranded DNA, and in particular their use in real time DNA amplification and quantification reactions. The invention further describes compositions including the probes suitable for the described uses and methods.

The application discloses a method, a primer group and a kit for whole genome sequencing of respiratory syncytial virus, and belongs to the technical field of DNA detection.The method comprises the following steps: S1, using first round amplification primers with sequences shown in SEQ ID NO.1 to SEQ ID NO.35 in table 1 to perform first round amplification with respiratory syncytial virus cDNA as a template; and S2, using second round amplification primers with sequences shown in SEQ ID NO.36 to SEQ ID NO.227 in table 2 to perform second round amplification with the first round amplification product as a template.The average length of data sequenced by the method is 0.8 kb, and the uniformity of respiratory syncytial virus coverage is greatly improved.

This invention pertains in general to probes for detecting and / or quantifying double-stranded DNA. The invention particularly relates to luminescence based probes, and methods of using such probes to detect or quantify double-stranded DNA, and in particular their use in real time DNA amplification and quantification reactions. The invention further describes compositions including the probes suitable for the described uses and methods.