111 results about "Dna detection" patented technology

The invention discloses a kit for detecting tomato brown crinkled fruit virus based on enzyme-mediated dual-amplification nucleic acid amplification and a detection method. The detection system comprises a primer pair DNA (deoxyribonucleic acid) upstream primer (F5) and a DNA downstream primer (R6) for amplifying the tomato brown crinkled fruit virus movement protein gene, a probe (RNA primer (RNA1)) for detecting the MP gene, a nucleic acid amplification group enzyme system (recombinase, single-chain binding protein, polymerase and ATP (adenosine triphosphate) energy regeneration enzyme), a signal amplification group enzyme system (positioning enzyme and cutting enzyme), activating enzyme NTP, a buffer solution and RNase-free Water. The system composed of the freeze-drying protective agent is prepared into the pre-subpackaged freeze-dried balls. The kit disclosed by the invention can realize DNA detection of aM concentration, and besides rapid qualitative detection of the tomato brown crinkled fruit virus, early screening of the tomato brown crinkled fruit virus can also be realized due to high detection sensitivity.

The invention discloses a micro-fluidic DNA detection-based security key generation and authentication method, which comprises the following steps: S1, carrying out DNA detection on a biological sample through a micro-fluidic chip to obtain SNP and STR feature data; s2, performing unified coding on the SNP and STR feature data to form stable bit string representation; s3, processing the bit string representation by using a fuzzy extraction mechanism, and generating a consistent key material under the condition of permitting a detection error; s4, deriving a final symmetric key from the key material by using a key derivation function; and S5, performing security packaging, transmission and authentication on the final symmetric key by adopting a post-quantum cryptography mechanism. According to the security key generation and authentication method based on microfluidic DNA detection, a set of key generation and identity authentication scheme with instantaneity, high specificity and anti-quantum security is constructed.

The invention discloses a double Taqman detection probe primer group for trichina and sarcocystis and application of the double Taqman detection probe primer group. Relates to the technical field of parasite detection. The invention provides a detection probe primer group, a kit and application. According to the detection method established by the invention, an amplification curve has a good linear relationship; the sensitivity is high; the specificity is high; dNA detection on 12 parasites such as toxoplasma gondii, ascaris suum, cryptosporidium and pulmonary nematode does not have an amplification curve; agarose gel electrophoresis shows that the molecular mass of the obtained PCR product strip accords with expectation. The kit has the characteristics of simplicity in operation, high specificity and high sensitivity, can quickly and efficiently detect trichina and sarcocystis, and has guiding significance in quick laboratory diagnosis of infection of the trichina and sarcocystis.

The invention discloses a method, a primer group and a kit for sequencing an adenovirus whole genome, and belongs to the technical field of DNA (Deoxyribose Nucleic Acid) detection. The method for sequencing the whole genome of the adenovirus comprises the following steps: S1, carrying out a first round of amplification by adopting a first round of amplification primers with sequences as shown in SEQ ID NO.1-SEQ ID NO.93 in a table 1; and S2, carrying out a second round of amplification by adopting the second round of amplification primers with the sequences as shown in SEQ ID NO.94-SEQ ID NO.285 in the table 2. The average length of data sequenced by the method is 0.8 kb, compared with the short read length of a second-generation platform, the uniformity of adenovirus coverage can be greatly improved, meanwhile, the adenovirus type coverage is comprehensive, and the method can be widely popularized.

The invention relates to a DNA detection data synchronization and classification method based on a decision tree model, and belongs to the technical field of DNA data classification processing. And finally, generating a first synchronization strategy or a second synchronization strategy according to the file size information of the detection data after DNA classification and the real-time network communication data of the communication channel, and performing data synchronization according to the first synchronization strategy or the second synchronization strategy. According to the method, the Bayesian network is fused in the decision tree model, the situation that the decision tree model is not high in precision in the DNA detection data classification process can be optimized, the data transmission situation of the DNA detection data in the data synchronization process can be optimized, the DNA detection data can be synchronized to a medical platform in time, and the accuracy of the DNA detection data is improved. And the query efficiency of the DNA detection data is improved.

The present application relates to the field of DNA detection technology, and specifically to a kit, method and application for early screening and monitoring of HPV-related tumors. The present application is based on multiplex ddPCR and uses mixed probes to detect four HPV subtypes in plasma at one time. Compared with conventional detection technology, the kit is simple and safe to operate, non-invasive, low-cost, and has a short cycle; at the same time, the kit has higher detection sensitivity and strong specificity, with a minimum detection limit of only 250 copies / ml.

The invention discloses a Chinese rose petal fold whole genome SNP (Single Nucleotide Polymorphism) molecular marker combination and application thereof. 968 SNP markers obviously related to petal fold traits are screened out and distributed on 14 chromosomes by analyzing 358 parts of Chinese rose re-sequencing data and utilizing a plurality of software models to carry out whole genome association analysis. A liquid-phase gene chip is designed on the basis of the SNP loci, and plants with ideal petal folding characters can be rapidly screened out through DNA detection in the Chinese rose seedling stage. The liquid phase breeding chip has the advantages of high throughput detection, low cost, shortened breeding period and the like, can simultaneously detect a large number of SNP sites and reduce the cost, can predict future performance of characters in a seed or seedling stage, greatly shortens the breeding period, accelerates cultivation of a new Chinese rose variety with a unique petal rolling character, and has a wide application prospect. The requirements of the market for novel flower types are met, the ornamental value and market competitiveness of the Chinese rose variety are improved, and the development of the flower industry is promoted.

The application belongs to the technical field of molecular breeding of Brassica napus, and particularly relates to a SNP molecular marker related to the thousand seed weight of Brassica napus and application thereof. The SNP molecular marker is located at the position of 43,480,336 of the scaffold C05 chromosome of Brassica napus reference genome ZS11 v0. The site has T / G polymorphism, wherein the T allele is a favorable allele variation for increasing the thousand seed weight of Brassica napus. The SNP molecular marker can be used for screening high thousand seed weight potential materials at the seedling stage or early stage through DNA detection, improving the high yield breeding efficiency of Brassica napus, and can be used for screening high thousand seed weight materials of Brassica napus, molecular assisted selection breeding, evaluation of germplasm resources and improvement of yield-related traits.

The invention belongs to the technical field of biology, and particularly discloses a primer combination, a kit and a method for detecting invasive species pistia stratiotes in water by utilizing multiple PCR (Polymerase Chain Reaction). A primer design method is innovated, a pistia stratiotes multiplex PCR primer combination is screened out, the pistia stratiotes DNA can be detected in a natural water body in combination with an optimized PCR reaction system and conditions, the problem existing in environment eDNA detection is solved, multiplex PCR primers can be combined with environment DNA detection, and the diffusion condition of the pistia stratiotes can be monitored more accurately. The detection method can effectively save time and cost, is high in sensitivity, specificity and efficiency and low in professional requirement, has a better detection rate when the concentration of a target species is low, and can be judged through a gel chart.

The invention provides a high-throughput DNA (deoxyribonucleic acid) detection method, platform, probe composition and kit for hereditary hemoglobinopathy. The high-throughput DNA detection method comprises the following steps: S1, designing probes for detecting known and unknown mutations in abnormal hemoglobinopathy related genes according to a specific rule; s2, synthesizing a probe; s3, extracting genome DNA from peripheral blood of a patient, constructing a library, hybridizing and capturing a target sequence, recovering a target region library, and performing library quality detection; s4, loading the library into a sequencing platform for high-throughput sequencing to obtain sequencing original data; and S5, performing data analysis on the sequencing original data. According to the application, a probe with a special sequence is designed, and sequencing data is subjected to information analysis, so that multiple hemoglobin mutation types can be detected at the same time, the detection efficiency and coverage are greatly improved, missing detection is avoided, and differential diagnosis can be performed on diseases with similar clinical manifestations.

The present application provides a method and system for accurately distinguishing between micro-partitions configured with a target gene and micro-partitions configured with a pseudo gene, and counting the target gene with high precision in digital PCR using melting curve analysis. The method includes: a step of configuring DNA solution in multiple partitions; a step of performing nucleic acid amplification reaction; a step of changing the temperature of each partition and measuring the fluorescence intensity; a step of calculating the melting temperature of the double-stranded DNA for each partition; a step of counting the number of partitions for each type of DNA; a step of outputting the number of counted partitions for each type of DNA; and a step of distinguishing the first gene and the pseudo gene based on the melting temperature and the number of counted partitions.

The utility model provides a DNA (deoxyribonucleic acid) detecting and sampling device for a fishery culture water body. The DNA detecting and sampling device comprises a sampling sleeve, a fixed sleeve and a piston rod, the sampling sleeve comprises a fixed part at the upper part, a hollow part in the middle and inside and a sampling tube placing part at the lower part, and the fixed part at the upper part of the sampling sleeve is provided with a fixed sleeve; the fixed sleeve is fixedly mounted at the upper part of the sampling sleeve, and a through threaded groove is formed in the middle of the fixed sleeve and is communicated with the interior of the sampling sleeve; the piston rod penetrates through the fixed sleeve in a threaded manner and extends to the cavity in the inner wall of the sampling sleeve. According to the utility model, the length-adjustable sampling sleeve and the fixed sleeve are matched with the graduated scale, so that accurate positioning sampling of different water layer depths is realized; the whole structure is simple and reasonable, the operation is convenient, and the high-precision sampling requirement of DNA detection of fishery culture water is particularly met.

The invention relates to the technical field of DNA detection, and discloses a PCR amplification device which comprises a temperature control box and a heat preservation mechanism, a heating cavity is formed in the upper end of the temperature control box, a sample supporting plate is arranged at the top of the heating cavity, and a high-temperature chamber and a low-temperature chamber are arranged on the side, close to the bottom of the temperature control box, of the heating cavity; the high-temperature chamber is communicated with the heating cavity through a first water outlet formed in the bottom of the heating cavity, the low-temperature chamber is communicated with the heating cavity through a second water outlet formed in the bottom of the heating cavity, a first switch mechanism for controlling the first water outlet to be opened and closed is arranged on the temperature control box, and a second switch mechanism for controlling the second water outlet to be opened and closed is arranged on the temperature control box; the high-temperature chamber is communicated with the heating cavity through a first water pump, the low-temperature chamber is communicated with the heating cavity through a second water pump, the heating cavity is communicated with an air cooling mechanism, and the air cooling mechanism is used for pumping external air into the heating cavity to cool the sample tube. The method has the effect of improving the PCR amplification efficiency.

The invention relates to the technical field of molecular biology, in particular to a group of SSR (Simple Sequence Repeat) molecular markers closely linked with a maize fusarium verticillium ear rot resistant QTL (Quantitative Trait Locus) and a detection method. As an SSR molecular marker, nucleic acid fragment SSR-51 and SSR-56 molecular markers are closely linked with the maize fusarium verticillium ear rot resistant gene, the recombination rate is low, the marker stability is high, and the primer can be used for effective detection, so that the molecular marker can be directly used for breeding links such as maize germplasm resource improvement, selfing line breeding and hybrid seed selection; according to the method, the disease-resistant genotype is identified through DNA detection in the corn seedling stage, and plant diseases do not need to be waited; the method has the advantages of high efficiency in detection, small quantity of required DNA samples, low requirement on DNA, simplicity in operation, high reliability, great allelic difference and no need of radioisotope.

The utility model provides a novel DNA detection sampling specimen box, which relates to the technical field of specimen box equipment, and comprises a box body, one side of the box body is connected with a box door through a hinge, the bottom of the box body is provided with a supporting base, the top of the box body is provided with a rotating mechanism, and the rotating mechanism comprises a rotating rod arranged at the top of the box body. One end of the rotating rod extends into the box body, an electric telescopic rod is arranged at the top end of the rotating rod, handles are slidably arranged on the two sides of the electric telescopic rod, sliding grooves are formed in the bottoms of the two handles, sliding blocks are arranged in the two sliding grooves, springs are arranged at the bottoms of the sliding blocks, and limiting rods are arranged at one ends of the springs. Two limiting holes are formed in the top of the box body, a plurality of sampling tubes with different sizes can be stored, a single sampling tube can be taken independently, and the practicability of the device is improved.

The utility model discloses a high-efficiency DNA (deoxyribonucleic acid) enrichment device for on-site investigation. The enrichment device comprises a connecting part, a communicating pipe, a suction head, a swab, a limiting part and a sealing cover, the limiting part, the connecting part, the communicating pipe and the swab are sequentially connected, the suction head is connected to the connecting part, an extrusion cavity is formed in the suction head, the limiting part is located in the extrusion cavity, the limiting part can be broken to enable the extrusion cavity to communicate with the communicating pipe, and the sealing cover is arranged on the peripheries of the communicating pipe and the swab in a sleeving mode and connected to the connecting part. The enrichment device is provided with the limiting part and the extrusion cavity, when the enrichment device needs to be used, the limiting part can be broken off, and at the moment, a biological material evidence collection reagent in the extrusion cavity can be released into the swab through the communicating pipe by extruding the suction head; after wiping, a biological material evidence collection reagent can be recycled into the extrusion cavity under the action of negative pressure generated by extruding the suction head, so that all cast-off cells and DNA on the surface of a material evidence can be sufficiently and effectively eluted and adsorbed, the detection rate of DNA is favorably improved, and cross contamination is avoided.

The invention discloses a silver ion detection test strip as well as a preparation method and a detection method thereof. The test strip comprises a strip-shaped bottom plate as well as a sample pad, a combination pad, a reaction film and a water absorption pad which are sequentially connected and fixed on the strip-shaped bottom plate, the combination pad is combined with an AuNPs-TS detection probe; a detection line T and a quality control line C are arranged on the reaction film; the detection line T comprises a target binding sequence S1-DNA (Deoxyribose Nucleic Acid); the quality control line C comprises a non-target binding sequence S2-DNA (Deoxyribose Nucleic Acid); the S1-DNA is used for forming C-Ag < + >-C mismatch with silver ions in a test solution. And the sample pad is glass cellulose paper which is soaked in a 20mM Tris-HAc buffer solution. The sequence of the TS DNA detection probe is 5 'HS-CCCCCCTGGTGGGGTGG-3', the sequence of the S1 DNA probe is 5 '-Biotin-TTTTTCCTCCTCCTCTTACCACCACCCA-3', the sequence of the S2 DNA probe is 5 '-Biotin-TTTTTTTTTTTTTCCACCACCCA-3', the sequence of the TS DNA detection probe is 5 'HS-CCCCCCTGGTGGGTGG-3', and the sequence of the S2 DNA detection probe is 5 '-

The utility model relates to the technical field of forensic medicine DNA extraction, and discloses a sticking and collecting tool which comprises a storage tube, a tube cover is arranged at the top end of the outer side wall of the storage tube, a sticking and collecting swab is arranged on the inner wall of the storage tube and comprises a fixed tube, the fixed tube is connected to the inner wall of the storage tube in a sliding mode, and the tube cover is arranged on the inner wall of the storage tube. A connecting rod penetrates through the inner wall of the fixing pipe and is in sliding connection with the inner wall of the fixing pipe, a placing block is fixedly connected to the top end of the outer wall of the connecting rod, a cuboid sticking swab head is fixedly connected to the bottom end of the outer wall of the connecting rod, and the connecting rod is clamped to the inner wall of the fixing pipe through a limiting mechanism. In the utility model, the silicon rubber substrate can better fit the surface of an object attached with cast-off cells, and the cuboid swab head can be used for sticking the cast-off cells with five surfaces and is suspended and stored in the storage tube, so that the collection rate of trace biological material evidences can be improved, the collection and inspection material transfer loss can be reduced, and the DNA (Deoxyribonucleic Acid) detection rate can be further improved.

The invention provides a flow nucleic acid single molecule counting method based on a rolling circle amplification technology, and belongs to the technical field of DNA detection. The flow nucleic acid single molecule counting method based on the rolling circle amplification technology comprises the following steps: preparing a DNA sample to obtain a DNA sample to be detected; based on the DNA sample, primer probes are designed, and the primer probes comprise a lock ring probe, an amplification primer and a fluorescent probe; carrying out a rolling circle amplification reaction, namely an RCA reaction, based on the primer probe to obtain a rolling circle amplification product; and introducing the rolling circle amplification product into a flow type single molecule counter to carry out flow type counting to obtain the original concentration of the DNA sample. According to the invention, rolling circle amplification, namely RCA isothermal amplification, is ingeniously combined with a single molecule counting technology, so that high-precision detection and quantification of a specific sequence of a nucleic acid single molecule are realized, and the combination greatly improves the sensitivity, specificity and accuracy of nucleic acid detection.

The invention discloses a kit and a method for detecting Verticillium based on enzyme-mediated dual-amplification nucleic acid amplification, and relates to the field of agriculture and forestry science and technology and biomedicine. The kit containing the system provided by the invention can realize aM-level DNA detection, can realize early screening of verticillium fungi besides rapid qualitative detection of verticillium fungi, and has the advantages of extremely low omission ratio caused by false negative, convenient operation and simple steps.

The invention relates to the technical field of gene sequencing, and discloses a variable matrix spectrum unfolding method of a ten-color fluorescence Sanger sequencer. According to the method, electrophoresis is carried out by blending the same target dye spectrum, and a high-resolution camera is used for collecting a single-pixel fluorescence signal. And then, carrying out block combination calculation on fluorescence signal data, and dynamically adjusting the block size based on signal stability and priori knowledge to adapt to peak height balance of different fluorescent dyes. And a self-adaptive adjustment mechanism is introduced to optimize the partitioning process, and partitioning combinations are introduced into acquisition software. The fluorescence intensity is verified through actual biological sample amplification, and an integrated feedback control system automatically adjusts acquisition parameters, so that accurate identification of ten-color fluorescence signals is ensured, and the accuracy and stability of DNA detection are improved.

The invention relates to a nucleic acid amplification reaction, in particular to a primer, a DNA (deoxyribonucleic acid) detection method and a DNA detection kit in an asymmetric nucleic acid amplification reaction. Specifically, the present invention relates to a primer for introducing and amplifying a mutation into a nucleic acid to be detected, and a DNA detection method and a DNA detection kit using the primer, the primer comprising a mutation in the sequence thereof for introducing a mutation into the nucleic acid to be detected, the ratio of a single strand of a binding region of a probe to a nucleic acid obtained by amplifying the nucleic acid to be detected is increased at a temperature at which the nucleic acid obtained by amplifying the nucleic acid to be detected binds to the probe.

The present invention refers to a wheat plant, seed or part of it comprising event IND-ØØ412-7, a commodity product resulting from the seed, a recombinant DNA molecule comprised in event IND-ØØ412-7, DNA primers and probes useful for detecting event IND-ØØ412-7, DNA detection kit, method for producing a wheat plant tolerant to abiotic stresses and method for detecting the presence of DNA belonging to wheat event IND-ØØ412-7 in a sample.

The invention relates to a kit for early differential diagnosis of EBV-HLH and a method for auxiliary diagnosis of EBV-HLH. The kit comprises an EBV nucleic acid detection reagent and a miRNA-375 detection reagent. The kit provided by the invention is used for combined detection of EBV-DNA and miRNA-375 in a detection sample, and when an EBV-DNA detection result is greater than 5000 copies / mL and a miRNA-375 detection CT value is less than 35, suspected EBV-HLH is diagnosed. The invention not only provides a specific diagnostic index for EB infected diseases, but also serves as a reliable auxiliary diagnostic tool for EBV-HLH and other EB virus infection related diseases. When the kit provided by the invention is used for identifying and diagnosing EBV-HLH, the sensitivity and the accuracy are higher.

This invention provides a high-throughput DNA detection method, platform, probe composition, and kit for hereditary hemoglobinopathies. The high-throughput DNA detection method includes: Step S1: Designing probes according to specific rules to detect known and unknown mutations in genes related to abnormal hemoglobinopathies; Step S2: Synthesizing probes; Step S3: Extracting genomic DNA from the patient's peripheral blood, constructing a library, hybridizing and capturing the target sequence, recovering the target region library, and performing library quality testing; Step S4: Loading the library into a sequencing platform for high-throughput sequencing to obtain raw sequencing data; Step S5: Performing data analysis on the raw sequencing data. This application designs probes with specific sequences and performs bioinformatics analysis on the sequencing data, enabling simultaneous detection of multiple hemoglobin mutation types, significantly improving detection efficiency and coverage, avoiding missed detections, and allowing for differential diagnosis of diseases with similar clinical manifestations.

The present invention relates to a primer, a DNA detection method, and a DNA detection kit for use in nucleic acid amplification, and particularly in asymmetric nucleic acid amplification. More specifically, this invention relates to a primer for introducing a mutation into a test nucleic acid and amplifying the test nucleic acid, the primer contains, in its sequence, a mutation to be introduced into the test nucleic acid, so as to increase a proportion of single-strand forming bases in a probe binding region of the nucleic acid amplified from the test nucleic acid, at a temperature of the binding of the nucleic acid amplified from the test nucleic acid with the probe; and, a DNA detection method and a DNA detection kit with use of such primer.

The present invention relates to a DNA detection method and a DNA detection kit for analyzing a gene, and more specifically, to a DNA detection method and a DNA detection kit for detecting a gene using a fluorescent-labeled probe.

The application discloses a DNA sequencing and encryption method based on micro-fluidic technology, and the method is characterized in that: through grey correlation weight factor analysis and feature sequence analysis based on an ant colony algorithm, a DNA fragment with a relatively short length and sufficient feature content is optimized to construct a feature information library; DNA detection is completed through fluorescent coding microspheres and high-precision fluorescence detection technology, information is stored in a single-chip microcomputer after microsatellite repeat sequence analysis; finally, the information is transmitted to an MCU by using a Bluetooth module, and the information is stored in a decentralized manner by using an ECC elliptic curve encryption algorithm and a block chain, so that the permanent storage and calling verification of personal identity information are realized. The application can be widely applied to the technical field of micro-fluidic chips.

The invention discloses an Indel molecular marker related to the browning character of towel gourd pulp, a detection primer and application of the Indel molecular marker and the detection primer, and belongs to the field of molecular assisted genetic breeding. The Indel molecular marker is located at the 8045208 site of the No. 5 chromosome of a luffa cylindrica reference genome, the site has a 15bp insertion / deletion variation, and the genotype (homozygous insertion type / heterozygous vs. Homozygous deletion type) is highly consistent with the browning-resistant phenotype. A detection primer pair developed on the basis of the marker can accurately predict the browning characteristic of the fruit of the towel gourd through DNA detection in the early growth stage of the towel gourd, and transformation from phenotype selection to genotype selection is achieved. The invention provides a brand-new, accurate and reliable molecular tool for browning-resistant breeding of towel gourds.

This invention relates to the design of specific primers for buffalo (Bubalus bubalis) cytochrome b (Cytb) gene for the detection of buffalo DNA using conventional PCR method. The primer sequence of buffalo species specific Cytb gene consists of forward sequence 5'- TTAGTACTATTCGCACCCGACCTC-3' and reverse 5'- TCGTTGTTTGGATGTATGTAGCAG-3'. This primer sequence has an amplicon size of 216 bp. The primers were used in a conventional PCR process operated under the following conditions, initiation stage of denaturation at 95ºC for 60 seconds, denaturation stage at 95ºC for 15 seconds, annealing stage at 63ºC for 15 seconds, elongation at 72ºC for 10 seconds cycle, final elongation at 72ºC for 60 seconds and at 35 cycles. This invention aims to amplify buffalo species specifically using conventional PCR methods so that it can detect the presence of buffalo DNA in processed products, especially for processed buffalo skin crackers.