79 results about "Dna detection" patented technology

The invention discloses a kit for detecting tomato brown crinkled fruit virus based on enzyme-mediated dual-amplification nucleic acid amplification and a detection method. The detection system comprises a primer pair DNA (deoxyribonucleic acid) upstream primer (F5) and a DNA downstream primer (R6) for amplifying the tomato brown crinkled fruit virus movement protein gene, a probe (RNA primer (RNA1)) for detecting the MP gene, a nucleic acid amplification group enzyme system (recombinase, single-chain binding protein, polymerase and ATP (adenosine triphosphate) energy regeneration enzyme), a signal amplification group enzyme system (positioning enzyme and cutting enzyme), activating enzyme NTP, a buffer solution and RNase-free Water. The system composed of the freeze-drying protective agent is prepared into the pre-subpackaged freeze-dried balls. The kit disclosed by the invention can realize DNA detection of aM concentration, and besides rapid qualitative detection of the tomato brown crinkled fruit virus, early screening of the tomato brown crinkled fruit virus can also be realized due to high detection sensitivity.

The invention discloses a micro-fluidic DNA detection-based security key generation and authentication method, which comprises the following steps: S1, carrying out DNA detection on a biological sample through a micro-fluidic chip to obtain SNP and STR feature data; s2, performing unified coding on the SNP and STR feature data to form stable bit string representation; s3, processing the bit string representation by using a fuzzy extraction mechanism, and generating a consistent key material under the condition of permitting a detection error; s4, deriving a final symmetric key from the key material by using a key derivation function; and S5, performing security packaging, transmission and authentication on the final symmetric key by adopting a post-quantum cryptography mechanism. According to the security key generation and authentication method based on microfluidic DNA detection, a set of key generation and identity authentication scheme with instantaneity, high specificity and anti-quantum security is constructed.

The invention discloses a Chinese rose petal fold whole genome SNP (Single Nucleotide Polymorphism) molecular marker combination and application thereof. 968 SNP markers obviously related to petal fold traits are screened out and distributed on 14 chromosomes by analyzing 358 parts of Chinese rose re-sequencing data and utilizing a plurality of software models to carry out whole genome association analysis. A liquid-phase gene chip is designed on the basis of the SNP loci, and plants with ideal petal folding characters can be rapidly screened out through DNA detection in the Chinese rose seedling stage. The liquid phase breeding chip has the advantages of high throughput detection, low cost, shortened breeding period and the like, can simultaneously detect a large number of SNP sites and reduce the cost, can predict future performance of characters in a seed or seedling stage, greatly shortens the breeding period, accelerates cultivation of a new Chinese rose variety with a unique petal rolling character, and has a wide application prospect. The requirements of the market for novel flower types are met, the ornamental value and market competitiveness of the Chinese rose variety are improved, and the development of the flower industry is promoted.

The application belongs to the technical field of molecular breeding of Brassica napus, and particularly relates to a SNP molecular marker related to the thousand seed weight of Brassica napus and application thereof. The SNP molecular marker is located at the position of 43,480,336 of the scaffold C05 chromosome of Brassica napus reference genome ZS11 v0. The site has T / G polymorphism, wherein the T allele is a favorable allele variation for increasing the thousand seed weight of Brassica napus. The SNP molecular marker can be used for screening high thousand seed weight potential materials at the seedling stage or early stage through DNA detection, improving the high yield breeding efficiency of Brassica napus, and can be used for screening high thousand seed weight materials of Brassica napus, molecular assisted selection breeding, evaluation of germplasm resources and improvement of yield-related traits.

The invention belongs to the technical field of biology, and particularly discloses a primer combination, a kit and a method for detecting invasive species pistia stratiotes in water by utilizing multiple PCR (Polymerase Chain Reaction). A primer design method is innovated, a pistia stratiotes multiplex PCR primer combination is screened out, the pistia stratiotes DNA can be detected in a natural water body in combination with an optimized PCR reaction system and conditions, the problem existing in environment eDNA detection is solved, multiplex PCR primers can be combined with environment DNA detection, and the diffusion condition of the pistia stratiotes can be monitored more accurately. The detection method can effectively save time and cost, is high in sensitivity, specificity and efficiency and low in professional requirement, has a better detection rate when the concentration of a target species is low, and can be judged through a gel chart.

The invention provides a high-throughput DNA (deoxyribonucleic acid) detection method, platform, probe composition and kit for hereditary hemoglobinopathy. The high-throughput DNA detection method comprises the following steps: S1, designing probes for detecting known and unknown mutations in abnormal hemoglobinopathy related genes according to a specific rule; s2, synthesizing a probe; s3, extracting genome DNA from peripheral blood of a patient, constructing a library, hybridizing and capturing a target sequence, recovering a target region library, and performing library quality detection; s4, loading the library into a sequencing platform for high-throughput sequencing to obtain sequencing original data; and S5, performing data analysis on the sequencing original data. According to the application, a probe with a special sequence is designed, and sequencing data is subjected to information analysis, so that multiple hemoglobin mutation types can be detected at the same time, the detection efficiency and coverage are greatly improved, missing detection is avoided, and differential diagnosis can be performed on diseases with similar clinical manifestations.

The present application provides a method and system for accurately distinguishing between micro-partitions configured with a target gene and micro-partitions configured with a pseudo gene, and counting the target gene with high precision in digital PCR using melting curve analysis. The method includes: a step of configuring DNA solution in multiple partitions; a step of performing nucleic acid amplification reaction; a step of changing the temperature of each partition and measuring the fluorescence intensity; a step of calculating the melting temperature of the double-stranded DNA for each partition; a step of counting the number of partitions for each type of DNA; a step of outputting the number of counted partitions for each type of DNA; and a step of distinguishing the first gene and the pseudo gene based on the melting temperature and the number of counted partitions.

The utility model provides a DNA (deoxyribonucleic acid) detecting and sampling device for a fishery culture water body. The DNA detecting and sampling device comprises a sampling sleeve, a fixed sleeve and a piston rod, the sampling sleeve comprises a fixed part at the upper part, a hollow part in the middle and inside and a sampling tube placing part at the lower part, and the fixed part at the upper part of the sampling sleeve is provided with a fixed sleeve; the fixed sleeve is fixedly mounted at the upper part of the sampling sleeve, and a through threaded groove is formed in the middle of the fixed sleeve and is communicated with the interior of the sampling sleeve; the piston rod penetrates through the fixed sleeve in a threaded manner and extends to the cavity in the inner wall of the sampling sleeve. According to the utility model, the length-adjustable sampling sleeve and the fixed sleeve are matched with the graduated scale, so that accurate positioning sampling of different water layer depths is realized; the whole structure is simple and reasonable, the operation is convenient, and the high-precision sampling requirement of DNA detection of fishery culture water is particularly met.

The invention relates to the technical field of molecular biology, in particular to a group of SSR (Simple Sequence Repeat) molecular markers closely linked with a maize fusarium verticillium ear rot resistant QTL (Quantitative Trait Locus) and a detection method. As an SSR molecular marker, nucleic acid fragment SSR-51 and SSR-56 molecular markers are closely linked with the maize fusarium verticillium ear rot resistant gene, the recombination rate is low, the marker stability is high, and the primer can be used for effective detection, so that the molecular marker can be directly used for breeding links such as maize germplasm resource improvement, selfing line breeding and hybrid seed selection; according to the method, the disease-resistant genotype is identified through DNA detection in the corn seedling stage, and plant diseases do not need to be waited; the method has the advantages of high efficiency in detection, small quantity of required DNA samples, low requirement on DNA, simplicity in operation, high reliability, great allelic difference and no need of radioisotope.

The invention discloses a silver ion detection test strip as well as a preparation method and a detection method thereof. The test strip comprises a strip-shaped bottom plate as well as a sample pad, a combination pad, a reaction film and a water absorption pad which are sequentially connected and fixed on the strip-shaped bottom plate, the combination pad is combined with an AuNPs-TS detection probe; a detection line T and a quality control line C are arranged on the reaction film; the detection line T comprises a target binding sequence S1-DNA (Deoxyribose Nucleic Acid); the quality control line C comprises a non-target binding sequence S2-DNA (Deoxyribose Nucleic Acid); the S1-DNA is used for forming C-Ag < + >-C mismatch with silver ions in a test solution. And the sample pad is glass cellulose paper which is soaked in a 20mM Tris-HAc buffer solution. The sequence of the TS DNA detection probe is 5 'HS-CCCCCCTGGTGGGGTGG-3', the sequence of the S1 DNA probe is 5 '-Biotin-TTTTTCCTCCTCCTCTTACCACCACCCA-3', the sequence of the S2 DNA probe is 5 '-Biotin-TTTTTTTTTTTTTCCACCACCCA-3', the sequence of the TS DNA detection probe is 5 'HS-CCCCCCTGGTGGGTGG-3', and the sequence of the S2 DNA detection probe is 5 '-

The invention discloses a kit and a method for detecting Verticillium based on enzyme-mediated dual-amplification nucleic acid amplification, and relates to the field of agriculture and forestry science and technology and biomedicine. The kit containing the system provided by the invention can realize aM-level DNA detection, can realize early screening of verticillium fungi besides rapid qualitative detection of verticillium fungi, and has the advantages of extremely low omission ratio caused by false negative, convenient operation and simple steps.

The invention relates to the technical field of gene sequencing, and discloses a variable matrix spectrum unfolding method of a ten-color fluorescence Sanger sequencer. According to the method, electrophoresis is carried out by blending the same target dye spectrum, and a high-resolution camera is used for collecting a single-pixel fluorescence signal. And then, carrying out block combination calculation on fluorescence signal data, and dynamically adjusting the block size based on signal stability and priori knowledge to adapt to peak height balance of different fluorescent dyes. And a self-adaptive adjustment mechanism is introduced to optimize the partitioning process, and partitioning combinations are introduced into acquisition software. The fluorescence intensity is verified through actual biological sample amplification, and an integrated feedback control system automatically adjusts acquisition parameters, so that accurate identification of ten-color fluorescence signals is ensured, and the accuracy and stability of DNA detection are improved.

The present invention refers to a wheat plant, seed or part of it comprising event IND-ØØ412-7, a commodity product resulting from the seed, a recombinant DNA molecule comprised in event IND-ØØ412-7, DNA primers and probes useful for detecting event IND-ØØ412-7, DNA detection kit, method for producing a wheat plant tolerant to abiotic stresses and method for detecting the presence of DNA belonging to wheat event IND-ØØ412-7 in a sample.

The invention relates to a kit for early differential diagnosis of EBV-HLH and a method for auxiliary diagnosis of EBV-HLH. The kit comprises an EBV nucleic acid detection reagent and a miRNA-375 detection reagent. The kit provided by the invention is used for combined detection of EBV-DNA and miRNA-375 in a detection sample, and when an EBV-DNA detection result is greater than 5000 copies / mL and a miRNA-375 detection CT value is less than 35, suspected EBV-HLH is diagnosed. The invention not only provides a specific diagnostic index for EB infected diseases, but also serves as a reliable auxiliary diagnostic tool for EBV-HLH and other EB virus infection related diseases. When the kit provided by the invention is used for identifying and diagnosing EBV-HLH, the sensitivity and the accuracy are higher.

This invention provides a high-throughput DNA detection method, platform, probe composition, and kit for hereditary hemoglobinopathies. The high-throughput DNA detection method includes: Step S1: Designing probes according to specific rules to detect known and unknown mutations in genes related to abnormal hemoglobinopathies; Step S2: Synthesizing probes; Step S3: Extracting genomic DNA from the patient's peripheral blood, constructing a library, hybridizing and capturing the target sequence, recovering the target region library, and performing library quality testing; Step S4: Loading the library into a sequencing platform for high-throughput sequencing to obtain raw sequencing data; Step S5: Performing data analysis on the raw sequencing data. This application designs probes with specific sequences and performs bioinformatics analysis on the sequencing data, enabling simultaneous detection of multiple hemoglobin mutation types, significantly improving detection efficiency and coverage, avoiding missed detections, and allowing for differential diagnosis of diseases with similar clinical manifestations.

The present invention relates to a primer, a DNA detection method, and a DNA detection kit for use in nucleic acid amplification, and particularly in asymmetric nucleic acid amplification. More specifically, this invention relates to a primer for introducing a mutation into a test nucleic acid and amplifying the test nucleic acid, the primer contains, in its sequence, a mutation to be introduced into the test nucleic acid, so as to increase a proportion of single-strand forming bases in a probe binding region of the nucleic acid amplified from the test nucleic acid, at a temperature of the binding of the nucleic acid amplified from the test nucleic acid with the probe; and, a DNA detection method and a DNA detection kit with use of such primer.

The application discloses a DNA sequencing and encryption method based on micro-fluidic technology, and the method is characterized in that: through grey correlation weight factor analysis and feature sequence analysis based on an ant colony algorithm, a DNA fragment with a relatively short length and sufficient feature content is optimized to construct a feature information library; DNA detection is completed through fluorescent coding microspheres and high-precision fluorescence detection technology, information is stored in a single-chip microcomputer after microsatellite repeat sequence analysis; finally, the information is transmitted to an MCU by using a Bluetooth module, and the information is stored in a decentralized manner by using an ECC elliptic curve encryption algorithm and a block chain, so that the permanent storage and calling verification of personal identity information are realized. The application can be widely applied to the technical field of micro-fluidic chips.

The invention discloses an Indel molecular marker related to the browning character of towel gourd pulp, a detection primer and application of the Indel molecular marker and the detection primer, and belongs to the field of molecular assisted genetic breeding. The Indel molecular marker is located at the 8045208 site of the No. 5 chromosome of a luffa cylindrica reference genome, the site has a 15bp insertion / deletion variation, and the genotype (homozygous insertion type / heterozygous vs. Homozygous deletion type) is highly consistent with the browning-resistant phenotype. A detection primer pair developed on the basis of the marker can accurately predict the browning characteristic of the fruit of the towel gourd through DNA detection in the early growth stage of the towel gourd, and transformation from phenotype selection to genotype selection is achieved. The invention provides a brand-new, accurate and reliable molecular tool for browning-resistant breeding of towel gourds.

This invention relates to the design of specific primers for buffalo (Bubalus bubalis) cytochrome b (Cytb) gene for the detection of buffalo DNA using conventional PCR method. The primer sequence of buffalo species specific Cytb gene consists of forward sequence 5'- TTAGTACTATTCGCACCCGACCTC-3' and reverse 5'- TCGTTGTTTGGATGTATGTAGCAG-3'. This primer sequence has an amplicon size of 216 bp. The primers were used in a conventional PCR process operated under the following conditions, initiation stage of denaturation at 95ºC for 60 seconds, denaturation stage at 95ºC for 15 seconds, annealing stage at 63ºC for 15 seconds, elongation at 72ºC for 10 seconds cycle, final elongation at 72ºC for 60 seconds and at 35 cycles. This invention aims to amplify buffalo species specifically using conventional PCR methods so that it can detect the presence of buffalo DNA in processed products, especially for processed buffalo skin crackers.

The invention provides a detection method for residual DNA of CHO cells and application thereof, CHO amplification products with large, medium and small lengths can be amplified respectively through three groups of primer and probe compositions, so that the quantitative level of distribution of residual DNA fragments of CHO cells in a sample to be detected is judged, a QPCR detection platform is established based on the primer and probe compositions, and the detection method has the advantages of high sensitivity, high sensitivity, high sensitivity and the like. Comprising a QPCR detection kit, a QPCR detection method and an application in CHO cell residual DNA detection, a QPCR reaction system and a reaction procedure are optimized, the sensitivity and specificity of CHO cell residual DNA detection are improved, the sensitivity can reach 3fg / L, the feasibility of QPCR quantitative detection of CHO cell residual DNA fragment distribution is further verified through fragmentation experiments, and the application of the QPCR detection kit and the QPCR detection method in the CHO cell residual DNA detection is provided. The method provides a new technical means for evaluating the quality of biological products, and has important application value.

The invention relates to the technical field of micro-fluidic electrochemical detection, in particular to a multi-channel micro-fluidic chip for detecting various drug-resistant mycobacterium tuberculosis as well as a use method and application of the multi-channel micro-fluidic chip. The multi-channel micro-fluidic chip comprises a splitting area and a sample mixing area, the splitting area comprises a sample injection hole and a DNA splitting chamber which are communicated in sequence, the sample mixing area comprises a sample mixing chamber, and the DNA splitting chamber and the sample mixing chamber are communicated through a first micro-channel; the electrode array area comprises a plurality of reaction detection chambers and detection electrodes, the detection electrodes are in one-to-one correspondence with the reaction detection chambers and are in contact with the inner spaces of the reaction detection chambers, the electrodes are modified with signal amplification probes, Cas12a-crRNA compounds are preloaded on the surfaces of the electrodes, and the reaction detection chambers are communicated with the sample mixing chamber through a second micro-channel. Multiple channels are designed, synchronous detection of multiple drug-resistant genes is achieved, integration of clinical sample treatment, DNA acquisition, DNA thermal denaturation treatment and DNA detection is achieved, operation is easy and smooth, and clinical diagnosis and medication guidance of tuberculosis drug resistance can be well served.

Bead constructs of sizes in the nanometer to micrometer range with a primary functionalization of a lipid membrane with embedded anchor peptides are provided. The anchor peptides may be adapted for a secondary functionalization of active molecules that are bound to the anchor peptides by transpeptidation or similar process. The functionalized bead platform can be adaptable and used in many different applications including biochemical and cellular assays, molecular diagnostics such as protein-protein interactions, protein-DNA interactions, DNA detection, separations, purifications, imaging, and microfluidics.

The application belongs to the technical field of molecular biology, and particularly relates to a method for improving meat quality of mutton sheep by using a SNP site of a FASN gene. The method for improving meat quality of mutton sheep by using the SNP site of the FASN gene comprises the following steps: detecting whether a g.50277076C>T site mutation exists in a FASN gene in a genome of a mutton sheep to be detected; selecting a mutton sheep individual with a genotype of TT type to perform artificial insemination or intrabreed. The application uses the above-mentioned molecular marker as a reliable marker of meat quality of Mongolian sheep, establishes a technical system for DNA detection of the genotype, improves selection intensity, and accuracy and efficiency of breeding, and improves meat quality of the Mongolian sheep.

The present invention provides a primer pair for detecting a Mycobacterium genomic DNA, a detection reagent containing the primer pair of the present invention, and a method for detecting the Mycobacterium genomic DNA by using the primer pair, wherein the primer pair specifically binds to a sequence as shown in SEQ ID NO: 1.

The application discloses a method for adenovirus whole genome sequencing, a primer group and a kit thereof, and belongs to the technical field of DNA detection.The method for adenovirus whole genome sequencing comprises the following steps: S1, first round amplification is carried out by using first round amplification primers with sequences shown in SEQ ID NO.1 to SEQ ID NO.93 in Table 1; and S2, second round amplification is carried out by using second round amplification primers with sequences shown in SEQ ID NO.94 to SEQ ID NO.285 in Table 2.The average length of data sequenced by the method is 0.8 kb, compared with short read length of a second-generation platform, the uniformity of adenovirus coverage can be greatly improved, meanwhile, the adenovirus types are comprehensively covered, and the method can be widely promoted.

The application discloses a pathogen DNA detection optimization method based on deep learning, and belongs to the technical field of bioinformatics and deep learning. The first electric signal data and the first DNA sequence data of nanopore sequencing are acquired; the first electric signal feature vector is output through an electric signal feature enhancement extraction network; the first sequence feature vector is output through a sequence feature coding network; the first fusion feature vector is obtained through element-by-element weighted summation of a feature layer fusion network; the first pathogen existence probability value is output through a first pathogen classification network and compared with the first filtering threshold value 0.15 for rapid screening; the second fusion feature vector is obtained through a time sequence context coding network and a cross attention fusion network for the sample; the second pathogen existence probability value is output through a second pathogen classification network; and the detection is completed according to the application scene parameter selected 0.35, 0.50 or 0.65 determination threshold value. The application realizes the cooperative utilization of electric signal and sequence features and the dynamic adjustment of the detection strategy.

The invention relates to the technical field of plant molecular breeding, in particular to an indel marker PH-03-Idel-106 related to the plant height character of corn and application of the indel marker PH-03-Idel-106 related to the plant height character of the corn, the Indel marker is located in the area of the third chromosome Chr3: 163962052-163964052 of the corn and is a 7bp insertion / deletion polymorphism marker, a 7bp insertion sequence CTGAGGG exists in a high-stalk corn inbred line, and no insertion sequence exists in a dwarf corn inbred line. A matched specific primer pair PH-03-Indel-106F / R can specifically amplify a target area, 1.5% agarose gel electrophoresis detection is carried out, it is judged that the corn plant height marker is a dwarf haplotype when a 270bp band appears, it is judged that the corn plant height marker is a high-stalk haplotype when no band exists, and the marker can be used for molecular marker-assisted selection, breeding improvement and early identification and screening of the corn plant height character. Phenotypes can be accurately predicted through DNA detection in the seedling stage, and environmental interference is avoided. The method has the advantages of being high in specificity, simple and convenient to detect, low in cost, high in throughput and the like, the breeding period can be remarkably shortened, directional improvement of the plant height character is achieved, and the method has important significance on corn variety genetic breeding and plant type optimization.

The invention belongs to the technical field of virus detection, and discloses a reaction solution for DNA virus and RNA virus detection, the reaction solution is a reaction solution containing acetate, acetate salt is introduced as an ionic reaction system, the specificity of a one-step method RT-PCR amplification system is greatly improved, and the specificity of the one-step method RT-PCR amplification system is greatly improved. The amplification inhibition phenomenon of the residual reverse transcriptase activity on a subsequent DNA template is greatly reduced, so that the kit has a good effect on DNA detection while being capable of detecting the RNA virus.

The application discloses a preparation method of a large-diameter graphite diacetylene powder material. The large-diameter (5-10 mu m) graphite diacetylene powder material with a two-dimensional nanosheet structure is prepared by adopting a substrate-free system combined with a microwave reaction. Unlike the graphite diacetylene film material prepared in the prior art, the graphite diacetylene powder material has a wider application in ion batteries, electrocatalysis, DNA detection and the like.

The invention discloses a pomacea canaliculata environment DNA specificity detection line and a biomass evaluation method, the technical scheme is as follows: the pomacea canaliculata environment DNA specificity detection line comprises an upstream primer, a downstream primer and a probe, and the method uses the pomacea canaliculata environment DNA specificity detection line to detect the pomacea canaliculata environment DNA concentration and obtain the environment DNA detection quantity; and calculating the biomass of the pomacea canaliculata according to the obtained environmental DNA detection quantity, namely, detecting the environmental DNA concentration of the pomacea canaliculata, and calculating the biomass of the pomacea canaliculata according to the environmental DNA detection quantity by using a pomacea canaliculata biomass calculation formula. The method has the advantages that only the pomacea canaliculata is specifically detected, other freshwater snails cannot be detected wrongly, the problem of wrong detection is effectively solved, particularly, different haplotypes of various pomacea canaliculata are covered, the environmental DNA monitoring work efficiency of the pomacea canaliculata is improved, and the established biomass evaluation model based on environmental DNA degradation and shedding rate has the advantages that the environmental DNA degradation and shedding rate of the pomacea canaliculata can be effectively evaluated. The method can be successfully applied to field biomass evaluation of ampullaria gigas.