52 results about "Dna detection" patented technology

The invention discloses a micro-fluidic DNA detection-based security key generation and authentication method, which comprises the following steps: S1, carrying out DNA detection on a biological sample through a micro-fluidic chip to obtain SNP and STR feature data; s2, performing unified coding on the SNP and STR feature data to form stable bit string representation; s3, processing the bit string representation by using a fuzzy extraction mechanism, and generating a consistent key material under the condition of permitting a detection error; s4, deriving a final symmetric key from the key material by using a key derivation function; and S5, performing security packaging, transmission and authentication on the final symmetric key by adopting a post-quantum cryptography mechanism. According to the security key generation and authentication method based on microfluidic DNA detection, a set of key generation and identity authentication scheme with instantaneity, high specificity and anti-quantum security is constructed.

The application belongs to the technical field of molecular breeding of Brassica napus, and particularly relates to a SNP molecular marker related to the thousand seed weight of Brassica napus and application thereof. The SNP molecular marker is located at the position of 43,480,336 of the scaffold C05 chromosome of Brassica napus reference genome ZS11 v0. The site has T / G polymorphism, wherein the T allele is a favorable allele variation for increasing the thousand seed weight of Brassica napus. The SNP molecular marker can be used for screening high thousand seed weight potential materials at the seedling stage or early stage through DNA detection, improving the high yield breeding efficiency of Brassica napus, and can be used for screening high thousand seed weight materials of Brassica napus, molecular assisted selection breeding, evaluation of germplasm resources and improvement of yield-related traits.

The present application provides a method and system for accurately distinguishing between micro-partitions configured with a target gene and micro-partitions configured with a pseudo gene, and counting the target gene with high precision in digital PCR using melting curve analysis. The method includes: a step of configuring DNA solution in multiple partitions; a step of performing nucleic acid amplification reaction; a step of changing the temperature of each partition and measuring the fluorescence intensity; a step of calculating the melting temperature of the double-stranded DNA for each partition; a step of counting the number of partitions for each type of DNA; a step of outputting the number of counted partitions for each type of DNA; and a step of distinguishing the first gene and the pseudo gene based on the melting temperature and the number of counted partitions.

The utility model provides a DNA (deoxyribonucleic acid) detecting and sampling device for a fishery culture water body. The DNA detecting and sampling device comprises a sampling sleeve, a fixed sleeve and a piston rod, the sampling sleeve comprises a fixed part at the upper part, a hollow part in the middle and inside and a sampling tube placing part at the lower part, and the fixed part at the upper part of the sampling sleeve is provided with a fixed sleeve; the fixed sleeve is fixedly mounted at the upper part of the sampling sleeve, and a through threaded groove is formed in the middle of the fixed sleeve and is communicated with the interior of the sampling sleeve; the piston rod penetrates through the fixed sleeve in a threaded manner and extends to the cavity in the inner wall of the sampling sleeve. According to the utility model, the length-adjustable sampling sleeve and the fixed sleeve are matched with the graduated scale, so that accurate positioning sampling of different water layer depths is realized; the whole structure is simple and reasonable, the operation is convenient, and the high-precision sampling requirement of DNA detection of fishery culture water is particularly met.

The invention relates to the technical field of molecular biology, in particular to a group of SSR (Simple Sequence Repeat) molecular markers closely linked with a maize fusarium verticillium ear rot resistant QTL (Quantitative Trait Locus) and a detection method. As an SSR molecular marker, nucleic acid fragment SSR-51 and SSR-56 molecular markers are closely linked with the maize fusarium verticillium ear rot resistant gene, the recombination rate is low, the marker stability is high, and the primer can be used for effective detection, so that the molecular marker can be directly used for breeding links such as maize germplasm resource improvement, selfing line breeding and hybrid seed selection; according to the method, the disease-resistant genotype is identified through DNA detection in the corn seedling stage, and plant diseases do not need to be waited; the method has the advantages of high efficiency in detection, small quantity of required DNA samples, low requirement on DNA, simplicity in operation, high reliability, great allelic difference and no need of radioisotope.

The invention discloses a silver ion detection test strip as well as a preparation method and a detection method thereof. The test strip comprises a strip-shaped bottom plate as well as a sample pad, a combination pad, a reaction film and a water absorption pad which are sequentially connected and fixed on the strip-shaped bottom plate, the combination pad is combined with an AuNPs-TS detection probe; a detection line T and a quality control line C are arranged on the reaction film; the detection line T comprises a target binding sequence S1-DNA (Deoxyribose Nucleic Acid); the quality control line C comprises a non-target binding sequence S2-DNA (Deoxyribose Nucleic Acid); the S1-DNA is used for forming C-Ag < + >-C mismatch with silver ions in a test solution. And the sample pad is glass cellulose paper which is soaked in a 20mM Tris-HAc buffer solution. The sequence of the TS DNA detection probe is 5 'HS-CCCCCCTGGTGGGGTGG-3', the sequence of the S1 DNA probe is 5 '-Biotin-TTTTTCCTCCTCCTCTTACCACCACCCA-3', the sequence of the S2 DNA probe is 5 '-Biotin-TTTTTTTTTTTTTCCACCACCCA-3', the sequence of the TS DNA detection probe is 5 'HS-CCCCCCTGGTGGGTGG-3', and the sequence of the S2 DNA detection probe is 5 '-

The invention discloses a kit and a method for detecting Verticillium based on enzyme-mediated dual-amplification nucleic acid amplification, and relates to the field of agriculture and forestry science and technology and biomedicine. The kit containing the system provided by the invention can realize aM-level DNA detection, can realize early screening of verticillium fungi besides rapid qualitative detection of verticillium fungi, and has the advantages of extremely low omission ratio caused by false negative, convenient operation and simple steps.

The invention relates to a kit for early differential diagnosis of EBV-HLH and a method for auxiliary diagnosis of EBV-HLH. The kit comprises an EBV nucleic acid detection reagent and a miRNA-375 detection reagent. The kit provided by the invention is used for combined detection of EBV-DNA and miRNA-375 in a detection sample, and when an EBV-DNA detection result is greater than 5000 copies / mL and a miRNA-375 detection CT value is less than 35, suspected EBV-HLH is diagnosed. The invention not only provides a specific diagnostic index for EB infected diseases, but also serves as a reliable auxiliary diagnostic tool for EBV-HLH and other EB virus infection related diseases. When the kit provided by the invention is used for identifying and diagnosing EBV-HLH, the sensitivity and the accuracy are higher.

The application discloses a DNA sequencing and encryption method based on micro-fluidic technology, and the method is characterized in that: through grey correlation weight factor analysis and feature sequence analysis based on an ant colony algorithm, a DNA fragment with a relatively short length and sufficient feature content is optimized to construct a feature information library; DNA detection is completed through fluorescent coding microspheres and high-precision fluorescence detection technology, information is stored in a single-chip microcomputer after microsatellite repeat sequence analysis; finally, the information is transmitted to an MCU by using a Bluetooth module, and the information is stored in a decentralized manner by using an ECC elliptic curve encryption algorithm and a block chain, so that the permanent storage and calling verification of personal identity information are realized. The application can be widely applied to the technical field of micro-fluidic chips.

The invention discloses an Indel molecular marker related to the browning character of towel gourd pulp, a detection primer and application of the Indel molecular marker and the detection primer, and belongs to the field of molecular assisted genetic breeding. The Indel molecular marker is located at the 8045208 site of the No. 5 chromosome of a luffa cylindrica reference genome, the site has a 15bp insertion / deletion variation, and the genotype (homozygous insertion type / heterozygous vs. Homozygous deletion type) is highly consistent with the browning-resistant phenotype. A detection primer pair developed on the basis of the marker can accurately predict the browning characteristic of the fruit of the towel gourd through DNA detection in the early growth stage of the towel gourd, and transformation from phenotype selection to genotype selection is achieved. The invention provides a brand-new, accurate and reliable molecular tool for browning-resistant breeding of towel gourds.

This invention relates to the design of specific primers for buffalo (Bubalus bubalis) cytochrome b (Cytb) gene for the detection of buffalo DNA using conventional PCR method. The primer sequence of buffalo species specific Cytb gene consists of forward sequence 5'- TTAGTACTATTCGCACCCGACCTC-3' and reverse 5'- TCGTTGTTTGGATGTATGTAGCAG-3'. This primer sequence has an amplicon size of 216 bp. The primers were used in a conventional PCR process operated under the following conditions, initiation stage of denaturation at 95ºC for 60 seconds, denaturation stage at 95ºC for 15 seconds, annealing stage at 63ºC for 15 seconds, elongation at 72ºC for 10 seconds cycle, final elongation at 72ºC for 60 seconds and at 35 cycles. This invention aims to amplify buffalo species specifically using conventional PCR methods so that it can detect the presence of buffalo DNA in processed products, especially for processed buffalo skin crackers.

The invention relates to the technical field of micro-fluidic electrochemical detection, in particular to a multi-channel micro-fluidic chip for detecting various drug-resistant mycobacterium tuberculosis as well as a use method and application of the multi-channel micro-fluidic chip. The multi-channel micro-fluidic chip comprises a splitting area and a sample mixing area, the splitting area comprises a sample injection hole and a DNA splitting chamber which are communicated in sequence, the sample mixing area comprises a sample mixing chamber, and the DNA splitting chamber and the sample mixing chamber are communicated through a first micro-channel; the electrode array area comprises a plurality of reaction detection chambers and detection electrodes, the detection electrodes are in one-to-one correspondence with the reaction detection chambers and are in contact with the inner spaces of the reaction detection chambers, the electrodes are modified with signal amplification probes, Cas12a-crRNA compounds are preloaded on the surfaces of the electrodes, and the reaction detection chambers are communicated with the sample mixing chamber through a second micro-channel. Multiple channels are designed, synchronous detection of multiple drug-resistant genes is achieved, integration of clinical sample treatment, DNA acquisition, DNA thermal denaturation treatment and DNA detection is achieved, operation is easy and smooth, and clinical diagnosis and medication guidance of tuberculosis drug resistance can be well served.

The application belongs to the technical field of molecular biology, and particularly relates to a method for improving meat quality of mutton sheep by using a SNP site of a FASN gene. The method for improving meat quality of mutton sheep by using the SNP site of the FASN gene comprises the following steps: detecting whether a g.50277076C>T site mutation exists in a FASN gene in a genome of a mutton sheep to be detected; selecting a mutton sheep individual with a genotype of TT type to perform artificial insemination or intrabreed. The application uses the above-mentioned molecular marker as a reliable marker of meat quality of Mongolian sheep, establishes a technical system for DNA detection of the genotype, improves selection intensity, and accuracy and efficiency of breeding, and improves meat quality of the Mongolian sheep.

The application discloses a method for adenovirus whole genome sequencing, a primer group and a kit thereof, and belongs to the technical field of DNA detection.The method for adenovirus whole genome sequencing comprises the following steps: S1, first round amplification is carried out by using first round amplification primers with sequences shown in SEQ ID NO.1 to SEQ ID NO.93 in Table 1; and S2, second round amplification is carried out by using second round amplification primers with sequences shown in SEQ ID NO.94 to SEQ ID NO.285 in Table 2.The average length of data sequenced by the method is 0.8 kb, compared with short read length of a second-generation platform, the uniformity of adenovirus coverage can be greatly improved, meanwhile, the adenovirus types are comprehensively covered, and the method can be widely promoted.

The application discloses a pathogen DNA detection optimization method based on deep learning, and belongs to the technical field of bioinformatics and deep learning. The first electric signal data and the first DNA sequence data of nanopore sequencing are acquired; the first electric signal feature vector is output through an electric signal feature enhancement extraction network; the first sequence feature vector is output through a sequence feature coding network; the first fusion feature vector is obtained through element-by-element weighted summation of a feature layer fusion network; the first pathogen existence probability value is output through a first pathogen classification network and compared with the first filtering threshold value 0.15 for rapid screening; the second fusion feature vector is obtained through a time sequence context coding network and a cross attention fusion network for the sample; the second pathogen existence probability value is output through a second pathogen classification network; and the detection is completed according to the application scene parameter selected 0.35, 0.50 or 0.65 determination threshold value. The application realizes the cooperative utilization of electric signal and sequence features and the dynamic adjustment of the detection strategy.

The invention belongs to the technical field of virus detection, and discloses a reaction solution for DNA virus and RNA virus detection, the reaction solution is a reaction solution containing acetate, acetate salt is introduced as an ionic reaction system, the specificity of a one-step method RT-PCR amplification system is greatly improved, and the specificity of the one-step method RT-PCR amplification system is greatly improved. The amplification inhibition phenomenon of the residual reverse transcriptase activity on a subsequent DNA template is greatly reduced, so that the kit has a good effect on DNA detection while being capable of detecting the RNA virus.

The application discloses a preparation method of a large-diameter graphite diacetylene powder material. The large-diameter (5-10 mu m) graphite diacetylene powder material with a two-dimensional nanosheet structure is prepared by adopting a substrate-free system combined with a microwave reaction. Unlike the graphite diacetylene film material prepared in the prior art, the graphite diacetylene powder material has a wider application in ion batteries, electrocatalysis, DNA detection and the like.

The invention discloses a pomacea canaliculata environment DNA specificity detection line and a biomass evaluation method, the technical scheme is as follows: the pomacea canaliculata environment DNA specificity detection line comprises an upstream primer, a downstream primer and a probe, and the method uses the pomacea canaliculata environment DNA specificity detection line to detect the pomacea canaliculata environment DNA concentration and obtain the environment DNA detection quantity; and calculating the biomass of the pomacea canaliculata according to the obtained environmental DNA detection quantity, namely, detecting the environmental DNA concentration of the pomacea canaliculata, and calculating the biomass of the pomacea canaliculata according to the environmental DNA detection quantity by using a pomacea canaliculata biomass calculation formula. The method has the advantages that only the pomacea canaliculata is specifically detected, other freshwater snails cannot be detected wrongly, the problem of wrong detection is effectively solved, particularly, different haplotypes of various pomacea canaliculata are covered, the environmental DNA monitoring work efficiency of the pomacea canaliculata is improved, and the established biomass evaluation model based on environmental DNA degradation and shedding rate has the advantages that the environmental DNA degradation and shedding rate of the pomacea canaliculata can be effectively evaluated. The method can be successfully applied to field biomass evaluation of ampullaria gigas.

This invention discloses a DNA detection method based on LAMP and immunochromatography, belonging to the field of biomedical detection technology, including the following steps: S1, LAMP amplification and product labeling: adding sample dilution buffer to a LAMP reaction tube, and using FITC-labeled specific primers to perform LAMP isothermal amplification of the target DNA in the sample; S2, immunochromatographic reagent detection: using a double-antibody sandwich immunochromatography method, the FITC-labeled LAMP product is visualized and detected by colloidal gold; S3, result determination. This DNA detection method based on LAMP and immunochromatography can achieve efficient amplification of target DNA under isothermal conditions using LAMP primers, and label the amplified product by pre-incorporating FITC-labeled specific primers. Subsequently, the signal is visualized and read out using a double-antibody sandwich immunochromatography method with colloidal gold, thereby achieving highly sensitive, highly specific, and rapid visual detection of target DNA.

The invention provides a silver birch juice DNA cell detection device. The DNA detection device comprises an ultraviolet photometer, a limiting groove plate, a rotating mechanism and a detection mechanism, the limiting groove plate is fixedly arranged in the ultraviolet photometer, and a cover plate capable of protecting the rotating mechanism and the detection mechanism is rotationally mounted at the top of the ultraviolet photometer. The rotating mechanism comprises a mounting seat, a rotatable main rotating disc and a sliding plate capable of sliding back and forth, the sliding plate drives the mounting seat to slide from inside to outside of the ultraviolet photometer along the track of the limiting groove plate, double-vessel rotating detection is adopted, a reference vessel and a sample vessel only need to be put into the equipment at the same time before detection, repeated replacement is not needed, and the detection efficiency is greatly improved. According to the equipment, mechanical rotation detection is adopted, manual operation steps are reduced, if multiple samples need to be continuously detected, only the sample vessel needs to be replaced, the reference vessel can be placed for a long time (reference liquid is not changed in extraction experiments of the same batch), the detection time of a single sample is greatly shortened, and errors caused by operation fatigue are reduced.

The invention discloses a fluorescent microsphere coding method for regulating and controlling FRET efficiency based on nucleic acid and application, and belongs to the technical field of DNA detection. The method comprises the following steps: combining DNA (deoxyribonucleic acid) coding probes with different lengths and two fluorescent probes marked with different fluorescent groups to the surfaces of magnetic beads through nucleic acid hybridization; the FRET efficiency of the two fluorophores is changed, so that the fluorescence intensity change on the carboxyl magnetic beads is realized; the method comprises the following steps: S1, combining a coupling probe with a microsphere: coupling a first coupling probe to the surface of a carboxylated magnetic bead through a carboxyl-amino covalent reaction; s2, hybridization reaction: taking magnetic beads obtained by coupling, adding a DNA coding probe, a first fluorescent probe and a second fluorescent probe, reacting for a period of time in a dark place, and washing to obtain coding microspheres; and S3, decoding: detecting the fluorescence intensity of FAM and Cy3 through a flow cytometry, and decoding according to the position difference of the microspheres in the two-dimensional scatter diagram. According to the invention, coding in two dimensions of wavelength and intensity can be realized.

Disclosed in the present invention are a nanopore protein monomer and a use thereof. The nanopore protein monomer is any one of the following polypeptides: (a1) a polypeptide having an amino acid sequence as set forth in SEQ ID NO: 4; (a2) a polypeptide derived from the amino acid sequence as set forth in SEQ ID NO: 4 by means of substitution and / or deletion and / or addition of one or more amino acids and having the same function; (a3) a polypeptide having 80% or above identity to the amino acid sequence defined in any one of (a1)-(a2) and having the same function; and (a4) a fusion polypeptide obtained after linking a tag to the terminus of the polypeptide defined in any one of (a1)-(a3). In the present invention, a nanopore protein which is stable and can be used for DNA detection is obtained on the basis of nanopores without a natural constriction region by a method of artificially designing a contraction region.

The present invention relates to: a primer for a nucleic acid amplification reaction, in particular, an asymmetric nucleic acid amplification reaction; a DNA detection method; and a DNA detection kit. Specifically, the present invention relates to: a primer for introducing a mutation into a subject nucleic acid and amplifying the nucleic acid, the primer comprising, in the sequence thereof, a mutation for introducing a mutation in the subject nucleic acid that results in, at the temperature at which a probe and a nucleic acid amplified from the subject nucleic acid are bonded, an increased ratio of the single-strand formation bases in the probe bonding region of the nucleic acid amplified from the subject nucleic acid; and a DNA detection method and a DNA detection kit which use the primer.

The invention discloses a method for detecting rabbit DNA residual quantity in a rabbit-derived biological product, and belongs to the field of DNA detection. The rabbit DNA in the recombinant human C1 esterase inhibitor expressed by a transgenic rabbit breast bioreactor platform can be effectively detected through the combination of specific primer sequences and probe sequences, and the rabbit DNA detection kit has good specificity, a large linear range and high accuracy, precision and sensitivity, and can be used for detecting the rabbit DNA in the recombinant human C1 esterase inhibitor expressed by the transgenic rabbit breast bioreactor platform. The method can be applied to detection of DNA residues in rabbit-derived biological products and related biological products, and has a good application prospect.

The invention discloses a DNA (Deoxyribose Nucleic Acid) molecule of a corn transformant ZD818 and a detection method. The transgenic corn event ZD818 is obtained by inserting an exogenous T-DNA (Transgenic Deoxyribose Nucleic Acid) containing insect-resistant genes cry2Ab and cry1Da and a herbicide-resistant gene cp4epsps into a specific site of a fifth chromosome of a corn genome; a nucleic acid sequence for specifically detecting the event comprises SEQ ID NO.1 or a complementary sequence thereof, and / or SEQ ID NO.2 or a complementary sequence thereof. Plants of the transgenic maize event ZD818 have high resistance to lepidoptera pests and high tolerance to glyphosate herbicides. The event-specific DNA detection method provided by the invention can accurately and stably identify the ZD818 event through nucleic acid amplification, and is of great significance to intellectual property protection, breeding application and safety supervision of the transgenic maize.

Embodiments of the present disclosure relate to six-nucleobase libraries having a third Watson-Crick base pair. Also provided herein are methods to prepare such six-nucleobase libraries, and their use for sequencing and modified nucleobase detection applications.

This invention discloses an optimized method for pathogen DNA detection based on deep learning, belonging to the interdisciplinary field of bioinformatics and deep learning. The invention acquires first electrical signal data and first DNA sequence data from nanopore sequencing; outputs a first electrical signal feature vector through an electrical signal feature enhancement extraction network; outputs a first sequence feature vector through a sequence feature encoding network; obtains a first fused feature vector by element-wise weighted summation through a feature layer fusion network; outputs the probability value of the presence of a first pathogen through a first pathogen classification network and compares it with a first filtering threshold of 0.15 for rapid screening; obtains a second fused feature vector for the samples using a temporal context encoding network and a cross-attention fusion network; outputs the probability value of the presence of a second pathogen through a second pathogen classification network; and completes the detection by selecting a judgment threshold of 0.35, 0.50, or 0.65 according to the application scenario parameters. This invention achieves the synergistic utilization of electrical signals and sequence features and dynamic adjustment of the detection strategy.

The application discloses a whole-genome SNP molecular marker combination related to the apraocha of sea cucumber against vibrio splendidus and application thereof, wherein the SNP molecular marker combination comprises 35 SNP sites, and in disease-resistant breeding, individuals with the 35 SNP site dominant genotypes can be screened to accelerate the breeding process of the disease-resistant traits. The molecular marker provided by the application has high biological correlation and prediction accuracy in breeding, and can be screened through DNA detection without relying on the phenotype of individual infection and disease, so that the generation interval is greatly shortened, the breeding efficiency is improved, the molecular marker assisted selection can overcome environmental interference, the genotypes are directly selected, the selection strength is high, the accuracy is high, and the genetic progress of the disease-resistant traits is accelerated.

The present invention relates to a primer, a DNA detection method, and a DNA detection kit for use in nucleic acid amplification, and particularly in asymmetric nucleic acid amplification. More specifically, this invention relates to a primer for introducing a mutation into a test nucleic acid and amplifying the test nucleic acid, the primer contains, in its sequence, a mutation to be introduced into the test nucleic acid, so as to increase a proportion of single-strand forming bases in a probe binding region of the nucleic acid amplified from the test nucleic acid, at a temperature of the binding of the nucleic acid amplified from the test nucleic acid with the probe; and, a DNA detection method and a DNA detection kit with use of such primer.

The invention discloses a six-color fluorescence combined calibrator, a fluorescence calibration method and application thereof, and relates to the technical fields of DNA detection, forensic genetics and biology. The kit comprises a first oligonucleotide, a second oligonucleotide, a third oligonucleotide, a fourth oligonucleotide, a fifth oligonucleotide and a sixth oligonucleotide, and the first oligonucleotide to the sixth oligonucleotide are marked with fluorophores. The labeling position of the fluorophore is located at the 5'end of the first oligonucleotide to the sixth oligonucleotide or at least one position in the oligonucleotide chain; the fluorophores on the first oligonucleotide to the sixth oligonucleotide are FAM, VIC, SX580, SX600, SX620 and SX650 in sequence. The synthesized fluorescent dye labeled oligonucleotide has the advantages of higher purity and few impurities, effectively reduces the generation of impure peaks during calibration, has stronger fluorescence signal response, and has better detection effect on DNA sequences which are not easy to amplify.