Multiplex PCR (Polymerase Chain Reaction) detection primer group for avian pathogenic escherichia coli and the like, method and kit

Abstract

The invention provides a multiplex PCR (Polymerase Chain Reaction) primer group for avian pathogenic escherichia coli, pasteurella multocida, proteus mirabilis, pseudomonas aeruginosa, salmonella andstaphylococcus aureus, a multiplex PCR detection kit comprising the primer group, and a multiplex PCR detection method using the primer group. The primer group comprises primer pairs PhoA-F and PhoA-Rfor specifically amplifying a gene phoA of the avian escherichia coli, primer pairs KMT-F and KMT-R for specifically amplifying a gene KMT1 of the pasteurella multocida, primer pairs AtpD-F and AtpD-R for specifically amplifying a gene AtpD of the proteus mirabilis, primer pairs PETA-F and PETA-R for specifically amplifying a gene PETA of the pseudomonas aeruginosa, primer pairs InvA-F and InvA-Rfor specifically amplifying a gene InvA of the salmonella, and prier pairs NuC-F and NuC-R for specifically amplifying a gene nuc of the staphylococcus aureus.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a multiple PCR primer set for detecting avian pathogenic Escherichia coli, Pasteurella multocida, Proteus mirabilis, Pseudomonas aeruginosa, Salmonella and Staphylococcus aureus, including The multiple PCR detection kit of the primer set and the multiple PCR detection method using the primer set. Background technique [0002] Avian pathogenic Escherichia coli (APEC), Pasteurella multocida, Proteus mirabilis, Pseudomonas aeruginosa, Salmonella and golden yellow Staphylococcus (Staphylococcus aureus) is the main pathogenic microorganism that endangers the poultry industry. [0003] After infecting poultry with avian pathogenic Escherichia coli, symptoms such as hepatomegaly, intestinal mucosal bleeding and ulcers, and pericardial inflammation appear. Extensive use will easily cause drug residues in poultry products, seriously affecting human health and causing public health problems....

AI Technical Summary

Problems solved by technology

PCR technology is widely used because of its strong specificity, high sensitivity, simple and fast operation, but these methods usually only detect one or a few pathogenic bacteria in each experiment

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