224 results about "Proteus mirabilis" patented technology

The invention discloses an isoflavone compound in a tobacco rhizome and a preparation method and application thereof. The preparation method comprises the following steps of: crushing a tobacco rhizome sample, performing ultrasonic extraction for 3 to 5 times by using 95 percent ethanol, combining the extracts, filtering, performing reduced pressure concentration to obtain an extract, primarily separating the extract by using silica gel column chromatography, further separating the extract by adopting high performance liquid chromatography, and thus obtaining the required new compound. Antibacterial activity screening test results show that the compound has strong antibacterial effect on common proteus species, escherichia coli, staphylococcus, bacillus subtilis and the like. The compound used for cigarette tipping paper plays an obvious role in inhibiting microbes for polluting the cigarette tipping paper.

The invention discloses a bacillus velezensis strain CSQXDZ26, which is bacillus velezensis (Bacillus velezensis) with the preservation number of CGMCC NO. 17935. The bacillus velezensis CSQXDZ26 hasremarkable antagonistic activity on bacterial plant diseases such as Xanthomonas oryzae pv. Oryzae, Xanthomonas oryzae pv. oryzicola, and xanthomonasaxonopodis pv. glycines AND THE LIKE; meanwhile, the bacillus velezensis CSQXDZ26 also has antibacterial activity on fungal plant diseases such as dithiorella gregaria, Fusarium graminearum, Fusarium oxysporum f.sp.Cucumerinum, Fusarium oxysporum f.sp. momordicae, Alternaria solani or Alternaria alternate, and also has excellent inhibition effect on common food spoilage bacteria such as bacillus subtilis, proteusbacillus vulgaris and staphylococcus aureus.

The invention provides a Bacillus coagulans HEW-B379 with a probiotic effect. The above strain is named as HEW-B379, and the preservation number of the strain is CGMCC No.12553. The Bacillus coagulans HEW-B379 has a substantial probiotic property, and can effectively inhibit growth breeding of enteropathogenic Escherichia coli, Staphylococcus aureus, Salmonella typhi, salmonella, Shigella, Proteus species, Shewanella putrefaciens and Pseudomonas aeruginosa. The Bacillus coagulans HEW-B379 has strong stress resistance, can resist high temperature and simulated gastric juice and simulate bile salt environment, can keep the survival rate of 99-100%, and can effectively adjust microbial balance of animal intestinal tracts, inhibit growth of harmful microbes, promote nutrition absorption of animals, improve the conversion rate of a feed and improve the productivity of the animals.

The subject matter of the present invention is a bacteria or mix of bacteria having an antagonistic activity with respect to stains of S. aureus, P. aeruginosa, Streptococcus pyogenes, Enterococcus faecium, Enterobacter cloacae, Proteus mirabilis, Bacteroides fragilis Staphylococcus epidermidis, Propionibacterium acnes, Candida albicans and/or Malassezia furfur as well as the use thereof in the treatment and/or prevention of infections or colonisations related to those pathogens. The invention pertains to care products containing one or more non-pathogenic antagonistic strains intended to prevent and/or treat infections or colonisations on skin, wounds, mucous membranes and appendages.

The present invention belongs to the field of microbe detecting technology. The oligonucleotide probe for detecting common intestinal tract pathogenic bacteria is designed on 16S rRNA and 23S rRNA of bacteria, ipaH of dysentery bacillus giant plasmid, VipR of Salmonella typhi and other gene sequence, has length of 25-50 bp, and relatively high sensitivity and specificity. The oligonucleotide probe is suitable for detection based on nucleic acid hybridization principle, especially detection based on gene chip principle. Under certain use condition, it can detect Listeria, parahemolutic vibrio, Campylobacter, etc. It may be used in many aspects, such as disease diagnosis, environment detection, food poisoning detection, etc.

A method and a composition for treatment of pulmonary bacterial infections caused by gram-negative bacteria suitable for treatment of infection caused by Escherichia coli, Klebsiella pneumoniae, Klebsiella oxytoca, Pseudomonas aeruginosa, Haemophilus influenzae, Proteus mirabilis, Enterobacter species, Serratia marcescens as well as those caused by Burkholderia cepacia, Stenotrophomonas maltophilia, Alcaligenes xylosoxidans, and multidrug resistant Pseudomonas aeruginosa, using a concentrated formulation of aztreonam lysinate delivered as an aerosol or dry powder formulation.

The present invention provides a gene chip for quickly detecting pathogens from food source, discloses the preparation method of said gene chip and provides 26 oligonucleotide probe sequences for detection. Said gene chip can quickly, accurately and high-effectively detect and identify the class of the pathogens in food, and its detection range includes staphylococcus aureus, Shiga's bacillus, salmonella, colibacillus 0157, bacillus proteus, mononuclear hyperplastic listerella, enterocolitis yersinia, aeruginous pseudomonads, vibrio parahaemolyticus, vibrio cholerae, bacillus cereus, beta hemolytic streptococcus, coconut fermentation pseudomonads, boticin, vibrio jejuni and bacillus perfringens, etc.

The invention provides a method for detecting fluorescent quantitative PCR of 12 pathogenic bacteria in real time at the same time, including the steps: collecting specific pathogenic gene or toxin gene of the target pathogen and using it as a target gene to design primers and probes so as to make the reaction conditions consistent; extracting a genome template of a sample to be detected; adding the template respectively into tubules equipped with different specific upstream and downstream primers and probes, and then adding the corresponding fluorescent quantitative PCR reagents; under the same cycle of fluorescent quantitative PCR, the corresponding primers and probes are used to detect the samples simultaneously, quickly and quantitatively in their respective reaction tubes. Easier, Quick and efficient, Twelve common pathogenic bacteria (Escherichia coli O157: H7, Listeria monocytogenes, Salmonella, Vibrio parahaemolyticus, Streptococcus betae, Yersinia enterocolitica, Streptococcusfaecalis, Shigella, Proteus mirabilis, Vibrio fluvialis, Campylobacter jejuni, Staphylococcus aureus) can be detected simultaneously in drinking water and food economically.

The invention discloses a specific nucleic acid marking sequence of proteus mirabilis, a PCR detecting method and the application. The aim of the invention is to provide the specific nucleic acid marking sequence of the proteus mirabilis, a qualitative and quantitative PCR detecting method of the proteus mirabilis and the application in preparing qualitative and quantitative PCR detecting kits of the proteus mirabilis. The specific nucleic acid marking sequence of the proteus mirabilis is one of following nucleic acid sequences: firstly, a DNA sequence of SEQ ID NO: 1 in the table, secondly, a restricted DNA sequence of the SEQ ID NO: 1 in the table, which has the homology more than 90% and is the specific nucleic acid sequence of the proteus mirabilis, and thirdly, a nucleic acid sequence which can cross-breed with the restricted DNA sequence of the SEQ ID NO: 1 in the table under the high-stringent condition. The invention can be used in qualitative and quantitative molecular detection of the proteus mirabilis (including the detecting methods of ordinary PCR, quantitative PCR, chips, hybrid and the like) and has broad application prospect.

The invention relates to a gene chip for detecting important pathogenic bacteria in an aquatic product and a kit thereof. The gene chip comprises a solid phase carrier and an oligonucleotide probe; the oligonucleotide probe comprises one or more of the following nucleotide sequences: (1) DNA (Deoxyribonucleic Acid) sequences selected from proteus mirabilis 1, proteus vulgaris, salmonella, vibrio parahaemolyticus, vibrio cholerae, Listeria monocytogenes, staphylococcus aureus, streptococcus pyogenes, vibrio vulnificus, bacillus cereus cluster, Shigella and pathogenic Y. enterocolitica ail gene; (2) complementary DNA sequences of the DNA sequences selected in the (1); (3) complementary RNA (Ribonucleic Acid) sequences of the DNA sequences in the (1) or (2). The kit comprises the gene chip. The gene chip and the kit can be used for detecting the important pathogenic bacteria in an aquatic product, are convenient to operate, high in precision and strong in repeatability.

The present invention relates to medicine technology and is improved mezlocillin sodium injection. The injection has one medicine weight ratio of mezlocillin sodium/Sulbactam sodium 1-16 to 1. It hasstrong antibacterial activity, wide antibacterial spectrum and thus high curative effect, and may be used in treating serious urinary tract infection, respiratory tract infection, intestinal tract, otorbinolaryngological infection, abdominal cavity infection, etc. caused by zymogenic staphylococcus, pneumococcus, enterococcus, purulent streptococcus, Hemophilus infuenzae, etc.

The invention discloses a preparation method for a dichloro substituted II-type halogenated polyketone compound zunyimycin A, wherein the compound is derived from a secondary metabolite of a streptomyces sp.FJS31-2 strain (with the strain preservation number of CGMCC4.7321), and finally, a structure is identified and determined after strain activation, expansion culture and isolation. The compoundcan be used in treatment of diseases infected by gram-positive bacteria such as staphylococcus aureus (MRSA) and drug-resistant bacteria thereof, staphylococcus epidermidis, bacillus subtilis and thelike, is used in treatment of diseases infected by gram-negative bacteria such as super-spectrum beta-lactamase escherichia coli (ESBL), proteusbacillus vulgaris, bacterium burgeri and the like, andis used for treatment of diseases infected by fungi, such as candida albicans.

The invention discloses a technology for producing phenylpyruvic acid by phenylalanine through enzymatic conversion and belongs to the technical field of biological engineering. According to the technology disclosed by the invention, a proteus mirabilis derived amino acid deaminase mutant is subjected to codon optimization and fermentation condition optimization and the enzyme activity of amino acid deaminase is remarkably improved. Furthermore, a conversion condition is optimized and the yield of the phenylpyruvic acid is remarkably improved; when the adding amount of wet strains is 25 to 30g/L, the yield of the phenylpyruvic acid can reach 81.1 to 83.0g/L and the mol conversion rate of the phenylpyruvic acid reaches 98.0 percent or mor.

The invention discloses 3-substituted-1-methyl-quinazoline-2,4-dione compounds shown in formula 1, a preparation method and application of the 3-substituted-1-methyl-quinazoline-2,4-dione compounds. Through in-vitro anti-microbial activity test, it is found that these compounds have certain inhibitory activity on gram-positive bacteria (Staphylococcus aureus, methicillin-resistant Staphylococcus aureus, Bacillus subtilis), gram-negative bacteria (Escherichia coli, proteusbacillus vulgaris, Pseudomonas aeruginosa) and fungus (Nostoc, Candida Albicans). Inhibitory activities of partial compounds on partial tested strains are approximate to or even superior to those of existing drugs such as streptomycin sulfate and polyoxin D. The compounds are especially sensitive to methicillin-resistant Staphylococcus aureus, can be prepared into antibacterial and/or anti-fungal drugs, and have the advantages of simple preparation method, easily-accessible materials and low cost.

A state, particularly a disease state, associated with the production of volatiles is detected by passing a sample containing the volatiles to a single sensor. This may be a semiconductor gas sensor element or a surface acoustic wave device. This provides an output signal, e.g. in the form of a tailing peak. A plurality of characteristics of the signal (e.g. peak height and maximum positive gradient) are measured to characterise the sample and hence the underlying state. For example we can discriminate between urine samples which are (a) infected with proteus, (b) infected with E. coli or (c) uninfected.

This invention relates to a preparation method of using proteus mirabilis to prepare biofloculation agent. The craft as follows: (1) subcuitivate Proteus mirabilis to slant culture group, preserve at freezer of 2 to 6 degC;(2) inoculate conservative strain to seed culture group, then prepare seed liquid in rocker incubator at 25 to 30 degC; (3) by proportion of 1 to 2% inoculate seed liquid to sixpenny nutrient medium, then embed it in the rocker, at 25 to 30degC, 120 to 140r/min, 1 to 2 days to obtain strain nutrient medium fermentation broth, take centrifugalization 20 to 40 min by 4000 to 6000r/ min centrifuge, remove sediment, the supernatant liquid is liquid biofloculation agent, measure flocculation ratio; (4) abstract and purify the liquid biofloculation to gain product. This invention has low cost, short production cycle, flocculation ratio can reach 92.88 to 93.13%, and has no secondary pollution, possess good generalization value.

The invention relates to the aplication of amigo-smile polyoses used for resisting bacteria or viruses, in particular to the application of the amigo-smile polyoses for preparing a medicament with the effects of resisting the bacteria or the viruses. The amigo-smile polyoses of the invention has inhibition effects to a plurality of bacteria or viruses, such as Bacillus subtilis, Staphylococcus aureus, Micrococcus luteus, Escherichia coli, salmonella, Proteus vuigaris, Shigella dysenteriae, Candida albicans or parainfluenza virus, and the like. The known method of the prior art can be adopted to prepare the amigo-smile polyoses of the invention into a medicament compound and the medicament compounds of any preparation formulation acceptable on pharmacy, such as granular formulation, oral liquid, capsule, troche, injection, pulvis, and the like.

The invention discloses a bacterial isolates relative to suppression of a biofilm and detoxification. The bacterial isolates relative to suppression of the biofilm and detoxification, which is provided by the invention, is especially Proteus mirabilis (Proteus mirabilis)10#, wherein the preservation number of Proteus mirabilis10# in China General Microbiological Culture Collection Center is CGMCC No.6426. The Proteus mirabilis10# CGMCC No.6426, which is provided by the invention, has effects of inhibiting formation of the pathogenic bacteria biofilm and detoxifying. The invention has the following advantages that: (1) through active ingredients (metabolites) of a Proteus mirabilis10# CGMCC No.6426 preparation, growth of the pathogenic bacteria is not influenced, the force is not generated, and the tolerance probability is low; and (2) through active ingredients (metabolites) of a Proteus mirabilis10# CGMCC No.6426 preparation, formation of the pathogenic bacteria biofilm is inhibited and the toxicity of the pathogenic bacteria is reduced.

The invention discloses an LAMP primer combination for detecting 8 environmental pathogens of dairy cow mastitis and application thereof. The primer combination provided by the invention is composed of 48 primers shown in sequence 1 to sequence 48. The primer combination provided by the invention can be used for detecting whether a to-be-detected bacterium is Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Streptococcus dysgalactiae, Streptococcus uberis, Salmonella typhimurium, Proteus mirabilis or Candida albicans, and can be used for detecting whether a to-be-detected sample contains Escherichia coli and/or Klebsiella pneumonia and/or Pseudomonas aeruginosa and/or Streptococcus dysgalactiae and/or Streptococcus uberis and/or Salmonella typhimurium and/or Proteus mirabilis and/or Candida albicans. The primer combination provided by the invention is used for identifying and detecting the 8 environmental pathogens of dairy cow mastitis, has high specificity and high sensitivity, and can realize simple, rapid and accurate detection, thus having great popularization value.

The invention discloses a proteus mirabilis strain V7 capable of eliminating ammonia nitrogen in water environment. The strain is preserved in the Chinese ordinary microorganism culture preservation and administration center of microbiology institute of Chinese Academy of Sciences on November 06th, 2010, and the preservation number is CGMCC No. 4312. The strain is capable of rapidly and efficiently lowering the concentration of ammonia nitrogen in low-salt and high-salt water environment, and ammonia monooxygenase (amoA) genes of the proteus mirabilis participate in the process that ammonia nitrogen is degraded by the proteus mirabilis.

The invention discloses a molecular beacon and a kit for quickly identifying various clinically common bacteria and belongs to the technical field of microbiological detection. The sequences SEQ ID of the molecular beacon are as shown in v6p1, v6p2, v1p1 and v1r. Through comparison between various clinically common bacteria 16s ribosome RNA sequences in an NCBI gene sequence database and design of the molecular beacon at a tag sequence, clinically common 18 bacteria, including baumanii, A.hydrophila, burkholderia cepacia, citrobacter freundii, enterobacter cloacae, enterococcus faecium, enterococcus faecalis, enterobacter aerogenes, escherichia coli, klebsiella pneumoniae, listeria monocytogenes, proteus mirabilis, pseudomonas aeruginosa, staphylococcus epidermidis, staphylococcus aureus, salmonella, serratia marcescens and stenotrophomonas maltophilia, can be quickly identified. The invention further discloses the kit containing the molecular beacon and used for detecting various bacteria, and the kit can quickly and accurately identify various clinically common bacteria.

He invention discloses a drug-resistant klebsiella pneumoniae phage RDP-KP-20004 and application thereof, wherein the preservation number of the phage is CGMCC No.21407, and the whole gene is sequenced and then is found to have no virulence gene and lysogenic gene, and the drug-resistant klebsiella pneumoniae phage RDP-KP-20004 provided by the invention has higher temperature tolerance and wider acid-base tolerance range, has strong cracking effect on pathogenic Klebsiella pneumoniae BK-20004 in a poultry breeding environment, and provides a phage source for the industrial production of phage for the prevention and treatment of the pathogenic Klebsiella pneumoniae BK-20004 in the poultry breeding environment; the klebsiella pneumoniae phage BK-20004 provided by the invention has very good advantages in treating diseases infected with klebsiella pneumoniae, and is relatively high in titer and good in prevention and treatment effect; and good application prospects are realized in the prevention and control of poultry pathogenic proteus vulgaris.

The invention relates to a method for artificially hatching chickens. According to the method, appropriate hatching eggs are selected, eggshells are disinfected by utilizing specially prepared disinfectant, the disinfectant prepared by mixing a sodium chlorate solution, a sodium thiosulfate solution, an elcoholic solution and toxifolin can sterilize escherichia coli, staphylococcus and Sabatier bacteria which exist on the surfaces of the eggshells, primary hatching is performed after the hatching eggs are disinfected, and conventional equipment is adopted to hatch the hatching eggs in primary hatching. The hatching process control is simple, the egg turning frequency is decreased, embryos can be effectively prevented from being adhered to eggshell membranes, the embryos can be promoted to absorb nutrient substances, wet spraying management is performed by spraying an antibacterial solution at the two ends of the hatching eggs, the interiors of the eggshells can be prevented from infecting bacteria and parasites, an inhibiting effect on typhoid bacillus, proteus and the staphylococcus is achieved, and meanwhile the eggshell hatching humidity is guaranteed; meanwhile, the method of spraying at the two ends of the hatching eggs is adopted, the humidity requirement of the hatching eggs can be met, hatching eggs breathing can be promoted, and steam exhausting of the hatching eggs is facilitated.

The invention discloses a gene chip for detecting 15 clinical common pathogenic microorganisms. The gene chip comprises specific detection probes fixed on a solid phase vector, wherein the probes are designed for 16SrRNA gene and ITS gene sequences of staphylococcus aureus, klebsiella pneumoniae, pseudomonas aeruginosa, proteus mirabilis, Escherichia coli, shigella, enterobacter aerogenes, pseudomonas fluorescens, candida albicans, candida glabrata, candida tropicalis, candida parapsilosis, aspergillus terreus, aspergillus flavus and rhizopus oryzae, and screened and verified by repeated tests, and have high hybridization specificity and accuracy. The chip provided by the invention ensures simple detection operations and relatively lower cost, saves time, is applied to the screening of the clinical common pathogenic microorganisms, the early rapid diagnosis of critical patients and the diagnosis of suddenly occurring infection event pathogens and favorable for clinically rapidly diagnosing infection sources and functions in the early detection and early diagnosis of clinical diseases.