43 results about "Epidemiologic survey" patented technology

This invention provides a double-antibody sandwich SA-ELISA detection kit for Fasciola hepatica infection in sheep. A double-antibody sandwich SA-ELISA method for detecting Fasciola hepatica CAg (cag) is established using mouse anti-Fasciola hepatica D38 monoclonal antibody and rabbit anti-Fasciola hepatica D38 polyclonal antibody. This detection uses monoclonal antibodies as coating antibodies, resulting in high specificity. Furthermore, the introduction of a biotin-streptavidin signal amplification system enhances sensitivity. It offers advantages such as high accuracy, good stability, simple operation, rapid detection, and easy interpretation, making it suitable for rapid clinical diagnosis and epidemiological surveys at the grassroots level.

This invention discloses an antibody that specifically recognizes Toxoplasma gondii surface antigens and its applications. The invention utilizes hybridoma technology to establish a hybridoma cell line by fusing Balb / C mouse spleen cells with SP2 / 0 cells. Monoclonal antibodies were prepared from these cells using in vitro culture and in vivo ascites induction methods, yielding the monoclonal antibody SH12-S7. This invention provides clinical and research institutions with an antibody that specifically recognizes Toxoplasma gondii surface antigens. It can be used for ELISA assays to detect clinical samples, for immunohistochemical assays to diagnose pathological specimens, and provides a convenient research tool for toxoplasmosis research departments. It has broad application value in the diagnosis and detection of definitive host infections and provides technical support for epidemiological investigations and evaluation of control effects of Toxoplasma gondii.

The application belongs to the technical field of biology and particularly relates to an ELISA kit for detecting chicken astrovirus type II antibodies and a preparation method. The Cell-ELISA experiment is carried out by using LMH cells infected with chicken astrovirus type II to detect the chicken astrovirus type II antibodies. The method has no cross reactivity with positive serum of Newcastle disease virus, avian influenza virus, chicken infectious anemia virus, avian leukosis virus, Marek's virus and chicken infectious bursal virus, which indicates that the method has good specificity. The Cell-ELISA method is used to detect the chicken serum sample to be detected by using whole virus infection, and is assembled into a kit for measuring chicken astrovirus antibodies in serum. The kit can be used for serological diagnosis of chicken astrovirus type II infection, monitoring of the level of antibodies, epidemiological investigation and the like, and provides a novel and effective detection means for prevention and treatment of chicken astrovirus type II.

The application discloses a parrot beak feather disease virus probe fluorescence quantitative detection primer, a kit and application thereof, and belongs to the technical field of virus detection. The detection primer comprises an upstream primer PBFDV-F with a nucleotide sequence as shown in SEQ ID NO. 3, a downstream primer PBFDV-R with a nucleotide sequence as shown in SEQ ID NO. 4 and a probe PBFDV-P with a nucleotide sequence as shown in SEQ ID NO. 5. The application designs parrot beak feather disease virus specific detection primers and Taq Man probes, constructs a recombinant standard plasmid, optimizes a reaction system and establishes a parrot beak feather disease virus detection method. The sensitivity, specificity and repeatability of the detection method are verified through experiments, and it is found that the detection method has good sensitivity, specificity and repeatability. The application provides reliable technical support for clinical diagnosis and epidemiological investigation of PBFD.

The invention discloses a food-borne case clustering analysis method and system based on multi-source heterogeneous data, and relates to the technical field of epidemiological investigation processing. According to the scheme, various heterogeneous data such as clinical detection and environment monitoring are integrated, accurate data processing is achieved through an exclusive process, high-quality case features are generated by means of a multi-level feature fusion strategy, reliable clustering is completed by means of a combined scheme of graph topology feature learning and contrast learning, and efficient dynamic iteration of a clustering result can be achieved for newly-added data. Compared with the prior art, the scheme has the advantages of complete data coverage, accurate processing, efficient fusion, reliable clustering and strong dynamic adaptability, and can provide powerful support for epidemiological investigation and real-time prevention and control of food-borne diseases.

The application belongs to the technical field of biological detection, and particularly relates to a double qPCR primer group for enterovirus C group 99 and enterovirus C group 96 and a detection method thereof. The kit realizes single-pipe double qPCR detection of EV-C99 and EV-C96, and the sensitivity of the kit to the two viruses reaches 10<2> copies / mL, which is superior to some commercial products. The kit has no cross reaction with close relative enteroviruses such as CVA8, CVA24, E6, PV, EVD68 and the like, and has good specificity. The coefficient of variation of the batch repeatability experiment is within 2.6%, and the detection result is stable. The whole amplification process is about 40 minutes, the operation is simple, and the kit is suitable for rapid screening in environmental samples or epidemiological investigation.

The application belongs to the technical field of virus detection, and discloses a sheep disease virus multiplex PCR detection primer, method and application. The base sequence of the primer FMDV-F of the sheep foot-and-mouth disease virus is SEQ ID NO:1, the base sequence of the primer FMDV-R is SEQ ID NO:2; the base sequence of the primer ORFV-F of the sheep orf virus is SEQ ID NO:3, the base sequence of the primer ORFV-R is SEQ ID NO:4; the base sequence of the primer GTPV-F of the goat pox virus is SEQ ID NO:5, and the base sequence of the primer GTPV-R is SEQ ID NO:6. The application optimizes the multiplex PCR, determines the optimal reaction system and the optimal reaction procedure, and can accurately detect single or mixed infection of the sheep foot-and-mouth disease virus, the sheep orf virus and the goat pox virus, and has an important role in epidemiological investigation and epidemic disease prevention and control.

The invention provides an RPA (recombinase polymerase amplification) detection kit, an RPA-LFD (lateral flow dipstick) detection system and an RPA-LFD detection method. The sequences of an upstream primer and a downstream primer used in the detection are respectively shown as SEQ ID NO. 1 and SEQ ID NO. 2; the sequence of the probe is as shown in SEQ ID NO.3, and tetrahydrofuran is inserted into the probe. The invention further provides a trypanosome RPA (recombinase polymerase amplification) detection kit and a trypanosome RPA-LFD (lateral flow dipstick) detection system containing the detection kit, and also provides a corresponding detection method. The whole detection process can be completed within 23 minutes, the operation is simple and convenient, and the result is visible. Compared with an existing detection method, the method has the advantages of being rapid, sensitive, high in specificity, suitable for on-site operation and the like, and is suitable for early diagnosis and epidemiological investigation of the trypanosomiasis in the large yellow croaker culture process.

The invention provides a method for detecting H3, H9 and H10 subtype avian influenza viruses through multiple real-time RT-PCR (Reverse Transcription-Polymerase Chain Reaction), and belongs to the technical field of molecular medicines. The primer group, the probe group, the kit and the like are provided, so that one-stop synchronous detection and accurate typing of avian influenza virus subtypes H3, H9 and H10 can be realized, and the detection efficiency is remarkably improved. The method has excellent specificity, can effectively avoid misjudgment, has extremely high detection sensitivity, can find virus traces in the early stage of infection, and strives for precious time for epidemic situation early warning. The method is convenient to operate, stable and reliable in result and easy to popularize and use, provides powerful technical support for daily monitoring and epidemiological investigation of the low-pathogenicity avian influenza in livestock and poultry farms, and has important practical application value for guaranteeing safe production of the breeding industry and preventing public health risks.

The invention provides a molecular diagnosis method for avian leukosis virus and Marek's disease virus mixed infection. The molecular diagnosis method comprises the following steps: (S01) collecting diseased material tissues; (S02) carrying out nucleic acid extraction on the sick material tissue of the sick chicken; (S03) detecting the nucleic acid by using specific primers of avian leukosis and Marek's disease; (S04) preparing a PCR (Polymerase Chain Reaction) detection system, and respectively adding ALV-env gene amplification primers and MDV-meq gene amplification primers for gene amplification; (S05) carrying out 1% agarose gel electrophoresis detection on the amplification product in the step (4), and carrying out sequence determination after determining a target band; (S06) carrying out BLAST comparison on the sequences obtained by sequencing, carrying out gene sequence analysis and drawing an evolutionary tree; and (S07) determining the virus type according to the homology and the evolutionary branches, so as to realize rapid diagnosis of ALV and MDV mixed infection, strain characteristic analysis and exclusion of other similar epidemic diseases, and provide technical support for accurate prevention and control of AL and MD in the poultry industry, provenance purification and epidemiological investigation.

The invention discloses a dual real-time fluorescent quantitative PCR (polymerase chain reaction) detection method for simultaneously detecting porcine hemagglutinated encephalomyelitis virus (PHEV) and porcine teschovirus (PTV), and belongs to the technical field of animal virus molecular biological detection. Aiming at the problems of similar clinical symptoms, high fatality rate and lack of synchronous detection and identification means after the suckling piglets are infected by PHEV and PTV, a specific primer pair and a probe are designed and screened through sequence comparison based on conserved regions of genomes of the two viruses. The detection method provided by the invention can realize qualitative and quantitative analysis of the two viruses, has the advantages of high sensitivity, high specificity and simplicity and convenience in operation, can quickly identify single infection or mixed infection of the two viruses, and provides an effective technical means for accurate detection in laboratories and epidemiological investigation.

The application discloses primers and a probe for real-time fluorescent quantitative PCR detection of a porcine epidemic diarrhea virus G2c subtype and application thereof and belongs to the field of animal virus molecular biology testing methods. The application compares S genes of different gene subtype strains of the porcine epidemic diarrhea virus, designs a pair of primers and a Taqman-MGB probe on the basis of a mutation site specific to the G2c subtype strain, and proves that the primers and the probe can effectively distinguish the G2c and other PEDV gene subtype strains through virus liquids and clinical samples of different gene subtype strains of the PEDV, meanwhile, the primers and the probe have no cross reaction to other viruses of the porcine enteric diarrhea syndrome, and the detection sensitivity of the kit can reach 5 copies / muL. The application can realize specific and sensitive detection of the G2c subtype, and has a positive significance for diagnosis and epidemiological investigation of the porcine epidemic diarrhea virus.

The present application belongs to the field of veterinary biotechnology detection, and particularly relates to a hybridoma cell strain for detecting or identifying MDV-1, a monoclonal antibody and application thereof. The present application takes pp38 protein of MDV-1 as an immunogen to screen a hybridoma cell strain MDV-1 Mab-pp38-E2 with a preservation number of CCTCC NO: C202501, and the monoclonal antibody generated by the hybridoma cell strain can recognize different serum type I chicken Marek's disease virus strains, does not have cross reactions with serum type II chicken Marek's disease virus, serum type III chicken Marek's disease virus, chicken egg drop syndrome virus (EDSV) and other common avian disease viruses, has good specificity and sensitivity, can be used for exogenous virus detection of serum type I chicken Marek's disease virus in avian virus live vaccines, and can also be used for clinical identification, virus content determination and epidemiological investigation of serum type I chicken Marek's disease virus.

The application discloses a monoclonal antibody against pigeon type I paramyxovirus, a hybridoma cell strain, a kit and application of the monoclonal antibody. The monoclonal antibody is secreted by the hybridoma cell strain 6B8 with a preservation number of GDMCC NO:68220, has a titer of 1:320000 and high specificity. The kit containing the antibody has optimal conditions that are as follows: an antigen coating concentration of 1 ug / mL, 1:25 dilution of a serum to be detected (37 DEG C for 60 minutes), 1:8000 dilution of a horseradish peroxidase-labeled monoclonal antibody (37 DEG C for 30 minutes), and determination of positivity by taking PI% greater than 27.74%. The kit has a sensitivity of 1:400, no cross reaction with positive serum of common avian pathogens, a clinical coincidence rate of 97.67%, and is independent of anti-pigeon IgY enzyme-labeled secondary antibodies. The application can be used for immune monitoring and epidemiological investigation of the pigeon type I paramyxovirus.

The invention discloses a dual-fluorescence PCR detection kit for simultaneously detecting clostridium septicum and clostridium ganodontotrichum and a primer probe group of the dual-fluorescence PCR detection kit, and belongs to veterinary detection of animal epidemic diseases in the technical field of biological detection. The invention discloses a dual-fluorescence PCR (polymerase chain reaction) detection kit for simultaneously detecting clostridium septicum and clostridium emphasis. The dual-fluorescence PCR detection kit comprises a primer probe group for detecting alpha genes of the clostridium septicum and a primer probe group for detecting the clostridium emphasis. The kit is simple and convenient in detection operation, strong in specificity, high in sensitivity and good in repeatability, and not only can be used for qualitatively detecting clostridium septicum and clostridium gangrene, but also can be used for realizing accurate quantification; technical support is provided for detection of clostridium septicum and clostridium gangrene, epidemic situation epidemiological investigation, epidemic disease monitoring and purification, strain screening and quality monitoring and stable production and supply conservation in the vaccine production process, and the method plays an important role.

The invention discloses preparation of an akabane disease virus N protein monoclonal antibody and a double-antibody sandwich ELISA (Enzyme-Linked Immunosorbent Assay) detection method, in order to prepare the akabane disease virus nucleocapsid protein monoclonal antibody, an eukaryotic recombinant plasmid pFastBac-AKAV-N is constructed and is converted into a DH10bac competent cell, and a recombinant stem Bacmid-AKAV-N for expressing AKAV N is successfully obtained. After the recombinant baculovirus is transfected to an sf9 insect cell, the recombinant baculovirus capable of stably expressing the AKAV N protein is rescued. A mouse and a rabbit are immunized with the identified and purified recombinant N protein, and a monoclonal antibody and a polyclonal antibody are prepared respectively. The titer of the polyclonal antibody is measured through an ELISA method, and two positive cell strains are obtained through screening and are named as 2G4 and 6C7 respectively. Furthermore, the prepared polyclonal antibody is used as a coating antigen, an HRP-labeled 2G4 monoclonal antibody is used as a detection antibody, and a double-antibody sandwich ELISA detection method for AKAV N protein antigen detection is optimized and established. According to the method, the AKAV N antigen can be specifically recognized, and reliable technical support is provided for clinical antigen monitoring of AKAV. The monoclonal antibody preparation system and the double-antibody sandwich ELISA detection method established by the invention lay an important foundation for pathogen detection and epidemiological investigation of akabane disease.

The application discloses a detection method for unknown pathogens and application thereof, and the method does not need to separate the unknown pathogens, only needs to perform metagenome sequencing on diseased animal lesion tissues, and then the primer and the probe for the unknown pathogens can be obtained through the method, efficient and convenient detection of the unknown pathogens is realized, and further, according to the detection result, the disease occurrence of a breeding farm and epidemiological investigation and prevention and control can be mastered, the spread of the unknown pathogens can be prevented and controlled in a short time, and the harm of the pathogens to the breeding farm is reduced.

The invention discloses a hygiene epidemiology work route planning method and system based on geographic information, and relates to the technical field of hygiene epidemiology and geographic information, and the method comprises the steps: carrying out the space-time alignment of multi-source heterogeneous data and geographic space data; performing risk factor screening based on a Pearson's correlation coefficient method according to the data after space-time alignment, and quantifying influence weights of risk factors in combination with a risk ratio model and a ratio ratio model; constructing a directed acyclic graph based on the risk factors and the influence weights; according to the directed acyclic graph, determining a conditional probability between nodes based on a Bayesian network, and further obtaining a risk thermodynamic diagram; constructing a multi-objective optimization function which aims at minimizing the total risk value, maximizing the resource coverage rate and minimizing the path cost; and dynamically generating a resource allocation path by adopting an improved A * algorithm in combination with the risk index layer, the risk thermodynamic diagram and the medical resource layer. According to the application, the epidemiological investigation efficiency and the prevention and control resource allocation accuracy can be improved.

The invention relates to the technical field of molecular detection, in particular to a kit for rapidly detecting toxin-producing clostridium difficile and high-yield strains. The invention provides a multiple primer probe group aiming at tcdB, cdtA and tcdC delta 117 of clostridium difficile, and a kit for rapidly detecting toxin-producing genes of the clostridium difficile. The kit can be used for rapidly and accurately detecting toxin-producing genes of clostridium difficile clinically, continuously detects toxin-producing clostridium difficile, can also detect RT027 type high-yield strains, and has important significance for clinically and accurately diagnosing infection of clostridium difficile and investigating epidemiology.

The invention relates to an epidemiological investigation method and system based on a large language model, and belongs to the technical field of public health information.The epidemiological investigation method comprises the steps that a structured knowledge base is constructed, and preprocessing, structured disassembly and feature extraction are conducted on multiple types of field data to form standardized data; fusing the processed data with a knowledge graph of a knowledge base, mining potential associated clues, inferring pathogenesis and propagation paths through a model, constructing the knowledge graph based on the knowledge base, developing an associated clue mining and pathogenesis inferring algorithm, training and optimizing the model through case data, and forming the knowledge graph covering event elements and causal relationships. According to the method, implicit experience dominance is realized, grassroots flow dispatchers can also achieve the clue capturing ability of qualified experts, and the working gap of flow dispatchers with different experience levels is reduced. The response speed is increased from the hour level to the second level for retrieval matching, the emergency disposal requirements of public health emergencies are met, and time is won for epidemic prevention and control.

The invention provides a primer and probe composition for typing detection of an infectious bronchitis virus. The composition is used for detecting genotypes of 4 / 91, TC07-2, LDT3, QX, LSC-99I and TW. The invention also provides a detection kit containing the primer and probe composition and a detection method. According to the invention, the detection of six genotypes is integrated into only two reaction tubes, the reaction steps are obviously reduced, the flux is improved, and a great leap is realized in multiple detection capability and detection efficiency, so that the kit is suitable for large-scale clinical screening and epidemiological investigation. The invention provides an unprecedented convenient and accurate tool for the diagnosis of complex clinical mixed infection by effectively identifying and combining the mixed infection of a plurality of genotypes through one-time reaction.

The application discloses a liver fluke colloidal gold detection method based on specific antigen FHH, which adopts liver fluke specific antigen gene FHH, FHH protein, colloidal gold particles, S. aureus protein A labeled colloidal gold particles and a detection test strip to perform detection. The method has the advantages of simplicity and rapidness, visual result, low cost, high specificity and high sensitivity. The antigen gene FHH obtained through screening of a liver fluke adult cDNA expression library guarantees the specificity of the application. The application is suitable for clinical rapid diagnosis and large-scale epidemiological investigation.

The invention relates to the technical field of animal epidemic disease epidemiological survey data analysis and processing systems, and discloses an animal epidemic disease epidemiological survey data analysis and processing system. The exploration data warehouse is used for reading survey data of animal epidemic situation epidemiology, the model library is used for storing various calculation models and algorithm formulas, and the component library is used for storing customer division data, customer loss data and customer product data of different industries. The mining algorithm library is used for performing mining calculation on data according to information of the model library, the component library and the exploration data warehouse and importing a calculation result into the survey data client, the knowledge storage end is used for receiving and storing the data imported by the data mining algorithm library, and the survey data client is used for reading the data of the knowledge storage end for query. The problems that a traditional data analysis and processing system is complex in architecture design structure and low in efficiency are solved.

The application discloses a visual multiple RT-LAMP detection method and primers for foot-and-mouth disease, vesicular stomatitis and blue tongue disease. A plurality of primers are designed, and specific primer combinations are optimized and screened. A fluorescent-quenching composite probe is added in the LAMP method for the first time, different fluorescent groups are marked at the 3' end of the probe, and finally, the multiple RT-LAMP detection method for differentially diagnosing FMDV, VSV and BTV in the same reaction tube is successfully established. The method has good specificity, high sensitivity, and can detect 1 copies / reaction viral RNA at the minimum, and the reaction time is short, and the detection can be completed in only 65 minutes. The result can be directly read by observing the color of the reaction product after the reaction, and the method can be used for clinical differential diagnosis and epidemiological investigation, and can be used for on-site quarantine in primary areas with poor conditions.

The invention relates to the technical field of epidemiological investigation, in particular to an epidemiological questionnaire processing method based on keyword recognition and structured extraction. Comprising the following steps: establishing an epidemiological knowledge graph, performing semantic modeling on infectious disease business knowledge, constructing a directed graph structure containing entities, attributes and relationships, and forming an epidemiological feature knowledge graph; defining an epidemiological investigation information label identification table, performing abstract analysis and association integration on information elements in an epidemiological investigation text record, and establishing a label system comprising a label type, a starting label name, a middle label name and an ending label name; establishing an epidemiological investigation auxiliary labeling library, selecting classical investigation records, performing pre-labeling in combination with the knowledge graph, and labeling entities, attributes and relationships according to rules of the label identification table; according to the method, the fundamental defect of the semantic analysis level in the prior art can be solved, and the intelligent and efficient level of flow scheduling work is improved.

The invention discloses a primer and a kit for detecting echinococcosis and application of the primer and the kit. The primer comprises a forward primer SEQ ID NO: 1 and a reverse primer SEQ ID NO: 2. The primer for detecting the echinococcosis and the corresponding detection method provided by the invention have the characteristics of good specificity and high sensitivity. Compared with conventional etiology and serology methods, the method has the advantages that the detection efficiency and universality are remarkably improved, five main subspecies can be covered at a time, and a reliable and practical technical means is provided for basic-level epidemiological investigation and precise monitoring of the echinococcosis.

This invention discloses a primer combination and its application for isothermal amplification-LFD rapid detection of porcine epidemic diarrhea virus (PEDV) G2 gene subtype, belonging to the field of animal viral molecular biology testing methods. This invention designs a primer combination targeting the S gene of PEDV. By setting a mismatched base at the 3' end characteristic base site of the upstream primer in this primer combination, the amplification specificity of the PEDV G2c gene subtype target sequence is significantly improved. Based on this primer combination, a rapid detection kit for isothermal amplification-LFD of PEDV G2c gene subtype was designed. This kit enables rapid identification and detection of PEDV G2c gene subtype strains, as well as specific and sensitive detection of the PEDV G2c gene subtype, which is of great significance for the diagnosis and epidemiological investigation of PEDV.

The invention relates to the technical field of animal epidemic disease diagnosis, and particularly discloses a specific primer group for detecting mycoplasma bovis. The specific primer group for detecting the mycoplasma bovis is selected from a first primer group or a second primer group, wherein the first primer group comprises an F3 primer, a B3 primer, an FIP primer and a BIP primer; the second primer group comprises an F3 primer, a B3 primer, an FIP primer and a BIP primer; the use method comprises the following steps: S1, extracting genome DNA of a sample to be detected to obtain a DNA template; s2, forming an LAMP reaction system; s3, carrying out isothermal amplification reaction on the LAMP reaction system; and S4, judging whether the mycoplasma bovis exists or not. The mycoplasma bovis specific primer group can be used for on-site rapid detection and epidemiological investigation of clinical samples, and has the advantages of high detection specificity, simplicity, convenience and rapidness in operation and high sensitivity.

The application discloses a different genotype goose astrovirus multiplex fluorescent PCR detection primer probe set, a kit and application, relates to the field of molecular biology; the primer probe set comprises a primer pair and a probe for detecting type 1 goose astrovirus (GoAstV-1) and type 2 goose astrovirus (GoAstV-2); the primer pair for detecting GoAstV-1 is as shown in SEQ ID NO. 1-2, and the probe is as shown in SEQ ID NO. 3; the primer pair for detecting GoAstV-2 is as shown in SEQ ID NO. 4-5, and the probe is as shown in SEQ ID NO. 6. The primer probe set disclosed by the application can realize multiplex fluorescent PCR detection of different genotypes of goose astrovirus, the detection method has good specificity, sensitivity and repeatability, and provides technical support for clinical diagnosis of GoAstV, determination of virus load and epidemiological investigation.

The invention discloses a target gene, a primer pair, a kit and a detection method for detecting multi-animal streptococcus and application, and relates to the technical field of bioengineering. The nucleotide sequence of the target gene for detecting the multi-animal streptococcus is as shown in SEQ ID NO: 1; the sequences of the primer pair designed based on the target gene are shown as SEQ ID NO: 2 and SEQ ID NO: 3; the fluorescent quantitative PCR kit for detecting the multi-animal streptococcus comprises a primer pair and a fluorescent quantitative PCR reaction premixed solution, the fluorescent quantitative PCR method for detecting the multi-animal streptococcus comprises the following steps: establishing a standard curve between a multi-animal streptococcus amplification CT value and a template copy number, and quantifying the multi-animal streptococcus in a sample to be detected through the standard curve. Clinical application proves that multi-animal streptococcus in mouse lung tissues and clinical swab samples can be effectively detected. The invention provides reliable technical support for rapid diagnosis and epidemiological investigation of multi-animal streptococcus.