79 results about "Epidemiologic survey" patented technology

The invention relates to the technical field of virtual simulation, and particularly discloses a sudden major respiratory infectious disease on-site epidemiological investigation virtual simulation experiment system which comprises a virtual scene management module, a data management module, a disease transmission simulation module, an epidemiological analysis module, a virtual on-site interaction module, a learning support module and an analysis and evaluation module. The virtual scene management module is used for creating and managing a simulation experiment environment and simulating an epidemic background and a development process of sudden major respiratory infectious diseases; the data management module is used for simulating the process of collecting case information, environment information and social contact data when students perform on-site epidemiological investigation; according to the system, big data analysis, the GIS technology and an AI intelligent analysis tool are integrated, a disease transmission path and a development trend are simulated, and virtual simulation not only helps students to master how to support a decision through scientific data, but also can improve the emergency response capability and the comprehensive analysis capability of the students in a complex epidemic situation.

The invention belongs to the field of veterinary biotechnology detection, and particularly relates to a hybridoma cell strain and a monoclonal antibody for detecting or identifying MDV-1 and application of the hybridoma cell strain and the monoclonal antibody. The hybridoma cell strain MDV-1Mab-pp38-E2 is obtained through screening by taking pp38 protein of MDV-1 as immunogen, the preservation number is CCTCC NO: C202501, the monoclonal antibody generated by the hybridoma cell strain can recognize I-type chicken Marek's disease virus strains with different serum, and the monoclonal antibody can be used for identifying the type I chicken Marek's disease virus strains with different serum types. The kit has good specificity and sensitivity and can be used for exogenous virus detection of the serum type I chicken Marek's disease virus in poultry virus live vaccines without cross reaction with serum type II chicken Marek's disease virus, serum type III chicken Marek's disease virus, chicken egg drop syndrome virus (EDSV) and other common poultry disease viruses, and the kit has good specificity and sensitivity and can be applied to exogenous virus detection of the serum type I chicken Marek's disease virus in poultry virus live vaccines. The kit can also be used for clinical identification, virus content determination and epidemiological investigation of serotype I chicken Marek's disease virus.

The invention provides a characteristic protein of serotype I chicken Marek's disease virus and detection application, and belongs to the technical field of virus detection. The invention provides a recombinant gene derived from serum I type chicken Marek's disease virus. The nucleotide sequence of the recombinant gene is shown as SEQ ID No. 1. The recombinant gene disclosed by the invention is used for coding the MDV-1 recombinant PP38 protein, and the MDV-1 recombinant PP38 protein can be applied to enzyme-linked immuno sorbent assay (ELISA) detection. The invention also provides an ELISA detection kit constructed by using the MDV-1 recombinant PP38 protein, and the ELISA detection kit can be used for clinical MDV-1 serum antibody detection, SPF chicken screening, exogenous virus antibody detection of poultry live vaccines or virus seeds, epidemiological investigation and the like, so as to prevent and control transmission of MDV-1 infection.

The invention provides an ultra-sensitive and high-specificity primer pair and crRNA for instantly detecting monkey pox virus, a kit and application thereof, and belongs to the technical field of molecular biological detection. The primer pair and the crRNA disclosed by the invention comprise a front primer, a rear primer and the crRNA. According to the primer pair and the crRNA disclosed by the invention, immediate detection of the MIRRA-CRISPR-Cas13a mediated monkey pox virus can be realized, the detection sensitivity is high, the specificity is strong, and rapid, accurate and sensitive identification of the monkey pox virus can be realized. Through implementation, experiments show that the detection limit of the primer pair and the crRNA for detecting the monkey pox virus can reach 14.4 copies / mL, and the primer pair and the crRNA have important significance on accurate clinical diagnosis of the monkey pox virus and epidemiological investigation.

The invention relates to the field of molecular biology, in particular to a primer probe combination for detecting genotype-2 goose astrovirus and a cdPCR kit. The nucleotide sequences of the primer and the probe are shown as SEQ ID NO.1-3. The cdPCR kit comprises the primer and probe combination. The cdPCR kit disclosed by the invention is applied to detection of GAstV-2, has the advantages of high sensitivity, strong specificity and stable repeatability, can realize rapid and accurate detection of early stage of GAstV-2 infection, and provides technical support for epidemiological investigation of GAstV-2 and early prevention and control of gout of goslings.

The invention belongs to the field of canine distemper virus detection, and particularly relates to a canine distemper virus RT-RAA CRISPR / Cas 12b detection kit and a detection method thereof. According to the kit, isothermal amplification is performed by using an RT-RAA universal primer, and then detection is performed by using a CRISPR / Cas 12b detection system consisting of target sequence sgRNA, so that amplification of a target fragment of the N gene of the canine distemper virus and target detection visualization can be effectively realized. The result of the embodiment shows that the RT-RAA CRISPR / Cas 12b detection kit finally designed by the invention is high in specificity and does not have cross reaction with other viruses, the lowest visual detection limit reaches 4.77 * 10 < 1 > copies / mu L, the virus can be instantly detected when the RT-RAA CRISPR / Cas 12b detection kit is used for detecting the CDV, the labor and equipment cost is low, the period is short, and the detection time does not exceed 2 hours. The rapid detection technology can be popularized and applied to epidemiological investigation and epidemic situation monitoring of the canine distemper virus, is suitable for epidemic disease monitoring of wild animals, quarantine of zoos and on-site rapid detection of clinical samples of canines, and has good practical significance and wide market prospects.

This invention provides a double-antibody sandwich SA-ELISA detection kit for Fasciola hepatica infection in sheep. A double-antibody sandwich SA-ELISA method for detecting Fasciola hepatica CAg (cag) is established using mouse anti-Fasciola hepatica D38 monoclonal antibody and rabbit anti-Fasciola hepatica D38 polyclonal antibody. This detection uses monoclonal antibodies as coating antibodies, resulting in high specificity. Furthermore, the introduction of a biotin-streptavidin signal amplification system enhances sensitivity. It offers advantages such as high accuracy, good stability, simple operation, rapid detection, and easy interpretation, making it suitable for rapid clinical diagnosis and epidemiological surveys at the grassroots level.

This invention discloses an antibody that specifically recognizes Toxoplasma gondii surface antigens and its applications. The invention utilizes hybridoma technology to establish a hybridoma cell line by fusing Balb / C mouse spleen cells with SP2 / 0 cells. Monoclonal antibodies were prepared from these cells using in vitro culture and in vivo ascites induction methods, yielding the monoclonal antibody SH12-S7. This invention provides clinical and research institutions with an antibody that specifically recognizes Toxoplasma gondii surface antigens. It can be used for ELISA assays to detect clinical samples, for immunohistochemical assays to diagnose pathological specimens, and provides a convenient research tool for toxoplasmosis research departments. It has broad application value in the diagnosis and detection of definitive host infections and provides technical support for epidemiological investigations and evaluation of control effects of Toxoplasma gondii.

The application belongs to the technical field of biology and particularly relates to an ELISA kit for detecting chicken astrovirus type II antibodies and a preparation method. The Cell-ELISA experiment is carried out by using LMH cells infected with chicken astrovirus type II to detect the chicken astrovirus type II antibodies. The method has no cross reactivity with positive serum of Newcastle disease virus, avian influenza virus, chicken infectious anemia virus, avian leukosis virus, Marek's virus and chicken infectious bursal virus, which indicates that the method has good specificity. The Cell-ELISA method is used to detect the chicken serum sample to be detected by using whole virus infection, and is assembled into a kit for measuring chicken astrovirus antibodies in serum. The kit can be used for serological diagnosis of chicken astrovirus type II infection, monitoring of the level of antibodies, epidemiological investigation and the like, and provides a novel and effective detection means for prevention and treatment of chicken astrovirus type II.

The invention provides an infectious bovine rhinotracheitis virus gD protein hybridoma cell strain, a monoclonal antibody and application of the monoclonal antibody in antigen detection, and belongs to the technical field of immunodetection. The invention provides an infectious bovine rhinotracheitis virus gD protein hybridoma cell strain IBRVMab-gD-8, and the preservation number of the infectious bovine rhinotracheitis virus gD protein hybridoma cell strain IBRVMab-gD-8 is CCTCC (China Center For Type Culture Collection) NO: The monoclonal antibody generated by the infectious bovine rhinotracheitis virus gD protein hybridoma cell IBRV Mab-gD-8 strain can specifically recognize strains of all genotypes of IBRV, does not generate cross reaction with viruses such as bovine viral diarrhea / mucosal disease virus (BVDV) and the like, and has good specificity; the kit not only can be used for exogenous virus detection (cell examination method) of IBRV in bovine virus live vaccines, but also can be used for clinical detection and epidemiological investigation of IBRV.

The invention discloses a primer, a TaqMan probe and a kit for carrying out fluorescent PCR (Polymerase Chain Reaction) detection on bovine-derived ureaplasma, and belongs to veterinary detection of animal epidemic diseases in the technical field of biological detection. The invention discloses a primer and a TaqMan probe which are used for carrying out fluorescent PCR (Polymerase Chain Reaction) detection on bovine-derived ureaplasma, and the primer and the TaqMan probe are an upstream primer U.diversum-F3, a downstream primer U.diversum-R3 and a TaqMan probe U.diversum-P3. The kit is simple and convenient in detection operation, strong in specificity, high in sensitivity and good in repeatability, and can be used for qualitatively detecting the bovine-derived ureaplasma; technical support is provided for detection of bovine-derived ureaplasma, epidemic situation epidemiological investigation, epidemic disease monitoring and purification, strain screening and quality monitoring and stable production and supply conservation in the vaccine production process, and the method plays an important role.

The application discloses a parrot beak feather disease virus probe fluorescence quantitative detection primer, a kit and application thereof, and belongs to the technical field of virus detection. The detection primer comprises an upstream primer PBFDV-F with a nucleotide sequence as shown in SEQ ID NO. 3, a downstream primer PBFDV-R with a nucleotide sequence as shown in SEQ ID NO. 4 and a probe PBFDV-P with a nucleotide sequence as shown in SEQ ID NO. 5. The application designs parrot beak feather disease virus specific detection primers and Taq Man probes, constructs a recombinant standard plasmid, optimizes a reaction system and establishes a parrot beak feather disease virus detection method. The sensitivity, specificity and repeatability of the detection method are verified through experiments, and it is found that the detection method has good sensitivity, specificity and repeatability. The application provides reliable technical support for clinical diagnosis and epidemiological investigation of PBFD.

The invention discloses a food-borne case clustering analysis method and system based on multi-source heterogeneous data, and relates to the technical field of epidemiological investigation processing. According to the scheme, various heterogeneous data such as clinical detection and environment monitoring are integrated, accurate data processing is achieved through an exclusive process, high-quality case features are generated by means of a multi-level feature fusion strategy, reliable clustering is completed by means of a combined scheme of graph topology feature learning and contrast learning, and efficient dynamic iteration of a clustering result can be achieved for newly-added data. Compared with the prior art, the scheme has the advantages of complete data coverage, accurate processing, efficient fusion, reliable clustering and strong dynamic adaptability, and can provide powerful support for epidemiological investigation and real-time prevention and control of food-borne diseases.

The application belongs to the technical field of biological detection, and particularly relates to a double qPCR primer group for enterovirus C group 99 and enterovirus C group 96 and a detection method thereof. The kit realizes single-pipe double qPCR detection of EV-C99 and EV-C96, and the sensitivity of the kit to the two viruses reaches 10<2> copies / mL, which is superior to some commercial products. The kit has no cross reaction with close relative enteroviruses such as CVA8, CVA24, E6, PV, EVD68 and the like, and has good specificity. The coefficient of variation of the batch repeatability experiment is within 2.6%, and the detection result is stable. The whole amplification process is about 40 minutes, the operation is simple, and the kit is suitable for rapid screening in environmental samples or epidemiological investigation.

The application belongs to the technical field of virus detection, and discloses a sheep disease virus multiplex PCR detection primer, method and application. The base sequence of the primer FMDV-F of the sheep foot-and-mouth disease virus is SEQ ID NO:1, the base sequence of the primer FMDV-R is SEQ ID NO:2; the base sequence of the primer ORFV-F of the sheep orf virus is SEQ ID NO:3, the base sequence of the primer ORFV-R is SEQ ID NO:4; the base sequence of the primer GTPV-F of the goat pox virus is SEQ ID NO:5, and the base sequence of the primer GTPV-R is SEQ ID NO:6. The application optimizes the multiplex PCR, determines the optimal reaction system and the optimal reaction procedure, and can accurately detect single or mixed infection of the sheep foot-and-mouth disease virus, the sheep orf virus and the goat pox virus, and has an important role in epidemiological investigation and epidemic disease prevention and control.

The present invention discloses an avian leukosis antigen detection kit and a preparation method thereof. The kit comprises an anti-avian leukosis virus P27 protein monoclonal antibody and a horseradish peroxidase-labeled sheep anti-P27 polyclonal antibody as a sandwich secondary antibody. The kit is based on a double-antibody sandwich method and has good specificity for A / B, J, and K subtype avian leukosis viruses prevalent in breeder chickens. The minimum detection limit is 1 ng / mL, the kit has strong anti-interference ability, a low false positive rate, and is easy to operate and highly stable. It is suitable for scenarios such as breeder chicken flock purification, SPF embryo quality monitoring, and epidemiological surveys. It effectively solves the problem of expensive imported products and limited domestic promotion, and has significant economic and social benefits.

The invention provides an RPA (recombinase polymerase amplification) detection kit, an RPA-LFD (lateral flow dipstick) detection system and an RPA-LFD detection method. The sequences of an upstream primer and a downstream primer used in the detection are respectively shown as SEQ ID NO. 1 and SEQ ID NO. 2; the sequence of the probe is as shown in SEQ ID NO.3, and tetrahydrofuran is inserted into the probe. The invention further provides a trypanosome RPA (recombinase polymerase amplification) detection kit and a trypanosome RPA-LFD (lateral flow dipstick) detection system containing the detection kit, and also provides a corresponding detection method. The whole detection process can be completed within 23 minutes, the operation is simple and convenient, and the result is visible. Compared with an existing detection method, the method has the advantages of being rapid, sensitive, high in specificity, suitable for on-site operation and the like, and is suitable for early diagnosis and epidemiological investigation of the trypanosomiasis in the large yellow croaker culture process.

The invention provides a method for detecting H3, H9 and H10 subtype avian influenza viruses through multiple real-time RT-PCR (Reverse Transcription-Polymerase Chain Reaction), and belongs to the technical field of molecular medicines. The primer group, the probe group, the kit and the like are provided, so that one-stop synchronous detection and accurate typing of avian influenza virus subtypes H3, H9 and H10 can be realized, and the detection efficiency is remarkably improved. The method has excellent specificity, can effectively avoid misjudgment, has extremely high detection sensitivity, can find virus traces in the early stage of infection, and strives for precious time for epidemic situation early warning. The method is convenient to operate, stable and reliable in result and easy to popularize and use, provides powerful technical support for daily monitoring and epidemiological investigation of the low-pathogenicity avian influenza in livestock and poultry farms, and has important practical application value for guaranteeing safe production of the breeding industry and preventing public health risks.

The invention provides a molecular diagnosis method for avian leukosis virus and Marek's disease virus mixed infection. The molecular diagnosis method comprises the following steps: (S01) collecting diseased material tissues; (S02) carrying out nucleic acid extraction on the sick material tissue of the sick chicken; (S03) detecting the nucleic acid by using specific primers of avian leukosis and Marek's disease; (S04) preparing a PCR (Polymerase Chain Reaction) detection system, and respectively adding ALV-env gene amplification primers and MDV-meq gene amplification primers for gene amplification; (S05) carrying out 1% agarose gel electrophoresis detection on the amplification product in the step (4), and carrying out sequence determination after determining a target band; (S06) carrying out BLAST comparison on the sequences obtained by sequencing, carrying out gene sequence analysis and drawing an evolutionary tree; and (S07) determining the virus type according to the homology and the evolutionary branches, so as to realize rapid diagnosis of ALV and MDV mixed infection, strain characteristic analysis and exclusion of other similar epidemic diseases, and provide technical support for accurate prevention and control of AL and MD in the poultry industry, provenance purification and epidemiological investigation.

The invention discloses a dual real-time fluorescent quantitative PCR (polymerase chain reaction) detection method for simultaneously detecting porcine hemagglutinated encephalomyelitis virus (PHEV) and porcine teschovirus (PTV), and belongs to the technical field of animal virus molecular biological detection. Aiming at the problems of similar clinical symptoms, high fatality rate and lack of synchronous detection and identification means after the suckling piglets are infected by PHEV and PTV, a specific primer pair and a probe are designed and screened through sequence comparison based on conserved regions of genomes of the two viruses. The detection method provided by the invention can realize qualitative and quantitative analysis of the two viruses, has the advantages of high sensitivity, high specificity and simplicity and convenience in operation, can quickly identify single infection or mixed infection of the two viruses, and provides an effective technical means for accurate detection in laboratories and epidemiological investigation.

The application discloses primers and a probe for real-time fluorescent quantitative PCR detection of a porcine epidemic diarrhea virus G2c subtype and application thereof and belongs to the field of animal virus molecular biology testing methods. The application compares S genes of different gene subtype strains of the porcine epidemic diarrhea virus, designs a pair of primers and a Taqman-MGB probe on the basis of a mutation site specific to the G2c subtype strain, and proves that the primers and the probe can effectively distinguish the G2c and other PEDV gene subtype strains through virus liquids and clinical samples of different gene subtype strains of the PEDV, meanwhile, the primers and the probe have no cross reaction to other viruses of the porcine enteric diarrhea syndrome, and the detection sensitivity of the kit can reach 5 copies / muL. The application can realize specific and sensitive detection of the G2c subtype, and has a positive significance for diagnosis and epidemiological investigation of the porcine epidemic diarrhea virus.

The present invention relates to the field of pathogen detection, in particular to a primer-probe set, gene chip, kit and application for detecting seven kinds of waterfowl infectious disease pathogens. The primer-probe set provided by the present invention can simultaneously detect goose parvovirus, duck viral enteritis virus, Muscovy duck parvovirus, duck hepatitis A virus type 1, duck hepatitis A virus type 3, duck Tembusu virus and novel duck reovirus, and has strong specificity and high sensitivity. Further, the gene chip prepared by using the primer-probe set provided by the present invention not only has strong specificity and high sensitivity (1.0×10<supgt;0< / supgt> copies / μL), but also has good repeatability and stability, can be stably stored for at least 180 days, and its sensitivity is also better than that of the standard fluorescence quantitative PCR method. It can quickly and efficiently co-detect the above-mentioned main pathogens of waterfowl diseases and can be used for the epidemiological investigation and disease diagnosis of waterfowl.

The present application relates to the technical field of molecular diagnosis, and discloses specific primers and a fluorescent PCR detection method for identifying Vibrio parahaemolyticus KUT2 type strains, and provides primers KUT2-F / R and a TaqMan probe for identifying Vibrio parahaemolyticus KUT2 type strains, and establishes a fluorescent PCR method (TaqMan probe method). The present application can quickly identify the main Vibrio parahaemolyticus KUT type prevailing in diarrhea patients in China, expand the Vibrio parahaemolyticus serotyping system, and provide a more accurate tool for epidemiological investigation and tracing. The present application has reached the level of the fluorescent PCR technology in terms of amplification efficiency, sensitivity and specificity, thereby realizing efficient and specific detection of KUT strains, and can judge whether the strain is the main KUT type prevailing in China within 1 hour, and fully meets the actual use detection requirements.

The present application belongs to the field of veterinary biotechnology detection, and particularly relates to a hybridoma cell strain for detecting or identifying MDV-1, a monoclonal antibody and application thereof. The present application takes pp38 protein of MDV-1 as an immunogen to screen a hybridoma cell strain MDV-1 Mab-pp38-E2 with a preservation number of CCTCC NO: C202501, and the monoclonal antibody generated by the hybridoma cell strain can recognize different serum type I chicken Marek's disease virus strains, does not have cross reactions with serum type II chicken Marek's disease virus, serum type III chicken Marek's disease virus, chicken egg drop syndrome virus (EDSV) and other common avian disease viruses, has good specificity and sensitivity, can be used for exogenous virus detection of serum type I chicken Marek's disease virus in avian virus live vaccines, and can also be used for clinical identification, virus content determination and epidemiological investigation of serum type I chicken Marek's disease virus.

The invention discloses a specific antigen fragment of salmonella typhimurium and application of the specific antigen fragment in detection of a salmonella typhimurium antibody. The amino acid sequence of the salmonella typhimurium specific antigen fragment is as shown in SEQ ID NO. 1. The invention discloses a method for detecting the indirect ELISA antibody of the salmonella typhimurium by taking the specific antigen fragment of the salmonella typhimurium as the coating antigen of the indirect ELISA for the first time, the specific antigen fragment of the salmonella typhimurium contains dominant B cell epitopes of the FliC protein of the salmonella typhimurium, and the specific antigen fragment of the salmonella typhimurium contains the dominant B cell epitopes of the FliC protein of the salmonella typhimurium. The salmonella typhimurium FliC protein antibody generated by induction can be specifically recognized, and the obvious synergistic effect is achieved in the aspect of improving the specific binding of the antigen and the antibody. The kit can be used for detecting the salmonella typhimurium antibody, and provides powerful technical support for epidemiological investigation of salmonella typhimurium and prevention and control of salmonella.

The invention provides a bovine rotavirus VP7 protein hybridoma cell strain, a monoclonal antibody and application of the monoclonal antibody in antigen detection, and belongs to the technical field of immunodetection. The invention provides a bovine rotavirus VP7 protein hybridoma cell BRV (bovine rotavirus) Mab-VP7-23 strain, and the preservation number of the BRV Mab-VP7-23 strain is CCTCC (China Center for Type Culture Collection) NO: C2025181. The monoclonal antibody generated by the bovine rotavirus VP7 protein hybridoma cell strain BRVMab-VP7-23 can specifically recognize strains of BRV G6, G8 and G10 genotypes, does not have cross reaction with viruses such as bovine viral diarrhea / mucosal disease virus (BVDV) and the like, has good specificity, can be used for exogenous virus detection (cell inspection method) of BRV in bovine virus live vaccines, and also can be used for detecting BRV in bovine virus live vaccines. The kit can also be used for clinical detection and epidemiological investigation of BRV.

The application discloses a monoclonal antibody against pigeon type I paramyxovirus, a hybridoma cell strain, a kit and application of the monoclonal antibody. The monoclonal antibody is secreted by the hybridoma cell strain 6B8 with a preservation number of GDMCC NO:68220, has a titer of 1:320000 and high specificity. The kit containing the antibody has optimal conditions that are as follows: an antigen coating concentration of 1 ug / mL, 1:25 dilution of a serum to be detected (37 DEG C for 60 minutes), 1:8000 dilution of a horseradish peroxidase-labeled monoclonal antibody (37 DEG C for 30 minutes), and determination of positivity by taking PI% greater than 27.74%. The kit has a sensitivity of 1:400, no cross reaction with positive serum of common avian pathogens, a clinical coincidence rate of 97.67%, and is independent of anti-pigeon IgY enzyme-labeled secondary antibodies. The application can be used for immune monitoring and epidemiological investigation of the pigeon type I paramyxovirus.

The invention discloses a dual-fluorescence PCR detection kit for simultaneously detecting clostridium septicum and clostridium ganodontotrichum and a primer probe group of the dual-fluorescence PCR detection kit, and belongs to veterinary detection of animal epidemic diseases in the technical field of biological detection. The invention discloses a dual-fluorescence PCR (polymerase chain reaction) detection kit for simultaneously detecting clostridium septicum and clostridium emphasis. The dual-fluorescence PCR detection kit comprises a primer probe group for detecting alpha genes of the clostridium septicum and a primer probe group for detecting the clostridium emphasis. The kit is simple and convenient in detection operation, strong in specificity, high in sensitivity and good in repeatability, and not only can be used for qualitatively detecting clostridium septicum and clostridium gangrene, but also can be used for realizing accurate quantification; technical support is provided for detection of clostridium septicum and clostridium gangrene, epidemic situation epidemiological investigation, epidemic disease monitoring and purification, strain screening and quality monitoring and stable production and supply conservation in the vaccine production process, and the method plays an important role.

The invention provides an RPA primer pair for detecting a duck plague virus virulent strain, a kit and a detection method thereof, and belongs to the technical field of biology. The invention provides an RPA primer pair for detecting a duck plague virulent strain. The RPA primer pair comprises a forward primer DEVF with a nucleotide sequence as shown in SEQ ID NO: 1 and a reverse primer DEV R with a nucleotide sequence as shown in SEQ ID NO: 2. The invention further provides an RPA primer probe set for detecting the duck plague virulent strain. The RPA primer probe set comprises the RPA primer pair and a probe DEV P, the nucleotide sequence of the probe DEVP is as shown in SEQ ID NO: 3. The RPA primer probe set has high specificity, specific detection of naturally infected duck plague virus virulent strains can be achieved, the primer pair is high in detection sensitivity, the detection limit is 0.1 fg / mu L, and the RPA primer probe set can be used for daily monitoring and epidemiological investigation of duck plague viruses of ducks.

The present invention discloses a mouse monoclonal antibody that recognizes unique specific B-cell epitopes in the spike protein S on the surface of the novel coronavirus and its applications. The monoclonal antibody includes a heavy chain and a light chain. The amino acid sequences of CDR1, CDR2, and CDR3 in the variable region of the heavy chain are SDFYIN, YISGNDHNNYTESFKS, and TSLDY, respectively. The amino acid sequences of CDR1, CDR2, and CDR3 in the variable region of the light chain are TASESISGSYLA, STTDLAS, and HQFHGSPWT, respectively. The mouse monoclonal antibody of the present invention is of the IgG2 subtype. Because it recognizes unique B-cell epitopes in the spike protein S of the novel coronavirus (SARS-CoV-2), it has strong specificity, high sensitivity, and good accuracy, and has been proven to be applicable to detections such as enzyme-linked immunosorbent assay (ELISA), western blot (WB), and immunofluorescence (IF). The mouse monoclonal antibody of the present invention can be used in the reagent kit of an automatic immunoanalyzer, and can also be applied to immunochromatographic test strips for detecting the spike protein S and virus particles on the surface of SARS-CoV-2, which is conducive to large-scale SARS-CoV-2 antigen detection and epidemiological investigations.