15 results about "Silver stain" patented technology

The invention belongs to the field of plant molecular genetics, and relates to a spirodela polymorphic microsatellite molecular marker primer and application thereof. The kit comprises 10 pairs of microsatellite primers which form 5 groups of duplex PCR systems, and the kit can be applied to population genetic diversity analysis of spirodela polyrhiza. The population genetic diversity analysis comprises the following steps: collecting a sample of spirodela polyrhiza to be identified; extracting spirodela polyrhiza genome DNA from the spirodela polyrhiza sample; taking the extracted DNA as a template, and carrying out PCR (Polymerase Chain Reaction) amplification on the DNA template by using the screened 10 pairs of microsatellite primers; carrying out electrophoresis detection on a PCR product, and then carrying out silver staining; the silver staining result is digitally analyzed, the UPGMA clustering analysis chart is drawn by genetic analysis software, the clustering analysis chart of the spirodela polyrhiza is identified according to the clustering analysis result, and the method has huge popularization and utilization value.

The invention discloses an SSR molecular marker primer related to leek dormancy as well as a detection method and application of the SSR molecular marker primer. Aiming at the problems that the traditional dormancy character identification of Chinese chives depends on field morphological observation as long as 4 months, the efficiency is low and special molecular markers are lacked, seven pairs of specific SSR primers (SSR005 / 170 / 249 / 325 / 423 / 478 / 610) are developed based on cross-species genome re-sequencing (taking green Chinese onion as a reference genome, and the comparison rate is 18.76%-18.89%) for the first time. The method comprises the following steps: establishing a matched detection system: rapidly extracting DNA (deoxyribonucleic acid) by a CTAB (cetyltrimethyl ammonium bromide) method, optimizing a PCR (polymerase chain reaction) system (20 mu L of DNA containing a template of 30 ng, 0.4 U of Taq enzyme, 0.2 mu M of primer, 2.0 mM of Mg < 2 + >, 0.2 mM of dNTPs, and annealing at 57 DEG C), and performing 8% polyacrylamide gel electrophoresis combined with silver staining development, thereby accurately identifying the dormancy character within 7 days. For example, the SSR423 primer has non-differentiated non-dormancy / dormancy germplasm at 177bp, and the coincidence rate of the detection result of 52 germplasm (such as dormancy germplasm 21-1 and non-dormancy germplasm 22-7) and the field phenotype reaches 100%.

The invention discloses a method for counting the number of wild rock sheep based on a microsatellite molecular marker. The method comprises the following steps: preprocessing a rock sheep manure sample and extracting DNA (Deoxyribonucleic Acid); performing double PCR amplification on the microsatellite marker, wherein each group of microsatellite primers comprises two pairs of specific primers; carrying out electrophoresis separation on a PCR product by using 12% polyacrylamide gel, and reading the size of an allele fragment of each microsatellite site through GeneMapper software after silver staining and developing; analyzing the acquired allele data by using software, and counting the number of different wild rock sheep individuals in the region according to an individual identification standard; the individual identification standard is as follows: if the genotypes of all 10 microsatellite loci are completely the same, or only one allele of one locus is different, the same individual is determined. The method is particularly suitable for non-invasive individual identification, genetic relationship identification and population quantity statistics of plateau wild rock sheep populations, and provides technical support for rock sheep resource protection and dynamic monitoring.

The application discloses a SSR molecular marker primer related to Chinese chives dormancy and a detection method and application thereof. In view of the problems that traditional Chinese chives dormancy trait identification relies on field morphological observation for 4 months, is inefficient and lacks special molecular markers, 7 specific SSR primers (SSR005 / 170 / 249 / 325 / 423 / 478 / 610) are developed based on cross-species genome resequencing (taking Chinese onion as a reference genome, and the alignment rate is 18.76%-18.89%) for the first time. Through the establishment of a matching detection system: CTAB method for rapid DNA extraction, optimization of the PCR system (20 muL contains 30 ng of template DNA, 0.4 U of Taq enzyme, 0.2 muM of primer, 0.2 mM of Mg 2+ 2.0 mM of dNTPs, 57 DEG C annealing), 8% polyacrylamide gel electrophoresis combined with silver staining development, the dormancy trait can be accurately identified within 7 days. For example, the SSR423 primer has no band at 177 bp, and the non-dormant / dormant germplasm cannot be distinguished. The detection results of 52 germplasms (such as dormancy germplasm 21-1 and non-dormancy germplasm 22-7) are consistent with the field phenotypes at a rate of 100%.

The invention discloses a method for detecting protein substances in a phlegm-heat clearing injection. The detection method comprises the following steps: (1) preparing a test solution; (2) preparing a sample adding sample solution; (3) preparing a reference solution; and (4) electrophoresis: respectively sucking the test sample solution, the sample adding sample solution and the reference substance solution, applying samples on gradient gel, and carrying out electrophoresis, silver staining and color development. The method provided by the invention is exclusive, sensitive, simple, convenient, efficient and rapid, can specifically detect the protein with the molecular weight of more than 5000, and provides a theoretical basis for the quality control of the phlegm-heat clearing injection and the deep research of the pharmacodynamic material basis through the detection of protein substances.

The invention provides a tissue staining method based on combination of antigen repair treatment and silver staining, which comprises the following steps: a) performing high-temperature thermal repair on a to-be-treated tissue slice by using an antigen repair liquid, cooling, and cleaning with water to obtain a repaired tissue slice; and b) performing silver staining on the repaired tissue slice obtained in the step a), dehydrating and sealing the slice to obtain the stained tissue slice. Compared with the prior art, the tissue staining method based on combination of antigen repair treatment and silver staining provided by the invention adopts specific process steps and conditions to realize relatively good overall interaction: firstly, the brain tissue is subjected to antigen repair treatment, and then the silver staining technology is adopted, so that the signal-to-noise ratio of an age pigment region is remarkably improved; compared with the prior art, an ideal staining effect is obtained, detail visibility in pathological detection can be effectively improved, the method helps to more accurately evaluate the morphology and distribution characteristics of the age pigment, and the method has a wide application prospect.

This invention discloses a method and system for in vitro assessment of renal biopsy tissue quality based on virtual special staining, belonging to the field of biomedical image processing technology. The method includes: acquiring and scanning undewaxed mounted sections to be assessed to obtain unstained whole-section images; inputting the unstained whole-section images into a pre-trained hybrid contrast virtual staining generation model to generate virtual special staining images with specific staining textures; the hybrid contrast virtual staining generation model is obtained through optimization training based on a total loss function, which includes adversarial loss, L1 loss, and PatchNCE block noise contrast estimation loss to maximize the mutual information between the input and generated images at corresponding spatial locations; inputting the virtual special staining images into a target detection network to identify glomerular structures and count the total number of glomeruli; and outputting the suitability assessment result of the undewaxed mounted sections based on the comparison result between the total number of glomeruli and a preset threshold. By scanning undewaxed mounted sections, the traditional dewaxing and rehydration steps are eliminated, improving efficiency; and the virtual silver staining technology significantly enhances the ability to identify glomeruli.

The present invention provides a rapid preparation method for ammoniacal silver staining samples of rumen ciliates, which is characterized by the following steps: preparing a solution; fixing rumen fluid; enriching ciliates; adding distilled water, enriched ciliates, pyridine and bacterial peptone solution into a centrifuge tube, reacting in the dark, adding aqueous formaldehyde solution, then adding Rio-Hortega solution for reaction, and adding Rio-Hortega solution for reaction again; taking out the centrifuge tube, standing or centrifuging, and sucking out the supernatant and storing it separately as a stored solution; adding distilled water into the centrifuge tube, discarding the supernatant, and adding the stored solution to obtain a silver-stained ciliate suspension. The method of the present invention is applicable to ammoniacal silver staining of ciliary patterns of various rumen ciliates, can efficiently, stably and qualitatively prepare ammoniacal silver staining samples of rumen ciliates, has the characteristics of simplicity, rapidity, good staining effect, clear staining conditions and high repeatability, and can maintain a good staining effect for a long time without permanent slide preparation operation, greatly simplifying the slide preparation cycle and saving raw material costs.

The application provides a tissue staining method based on antigen repair treatment combined with silver staining, comprising the following steps: a) high-temperature heat repair of a tissue section to be treated with an antigen repair solution, and washing with water after cooling to obtain a repaired tissue section; b) silver staining of the repaired tissue section obtained in step a), dehydration, and then mounting to obtain a stained tissue section. Compared with the prior art, the tissue staining method based on antigen repair treatment combined with silver staining provided by the application adopts specific process steps and conditions to realize overall good interaction: first, the brain tissue is subjected to antigen repair treatment, and then the silver staining technology is adopted, which significantly improves the signal-to-noise ratio of the senile plaque region, and ideal staining effect is obtained, which can effectively improve the detail visibility in pathological detection, help more accurately evaluate the morphology and distribution characteristics of the senile plaque, and has a wide application prospect.

The invention discloses a section for evaluating cancerosarcoma as well as a preparation method and application thereof, and belongs to the technical field of pathology. The preparation method comprises the following steps: reticular fiber dyeing: dyeing reticular fibers in section tissues by a silver staining method; and performing immunohistochemical staining: performing labeling staining on AE1 / AE3 protein in the section tissue by a non-histochemical staining method. According to the section obtained by the preparation method, two kinds of dyeing can be presented on one tissue section, the cancer, sarcoma and cancer and sarcoma expression effects are improved, the identification difficulty is reduced, and precise tumor typing is facilitated.

The invention provides a visual microarray specific nucleic acid probe combination, system and method for detecting antibiotic resistance genes, and belongs to the technical field of environmental microbiological detection. According to a positive-sense strand and an antisense strand of double-stranded DNA of a target antibiotic resistance gene, a carboxylation fixed probe and a sulfhydrylation signal probe are respectively designed, and initial signal multiplication is realized through double-strand double recognition; and carrying out secondary amplification in combination with nanogold-catalyzed silver staining deposition to form a visual detection signal. The sensitivity of the method reaches 10 <-8 > mol, antibiotic resistance genes such as qepA, macB and the like in soil can be detected at high throughput, and the method has the advantages of high specificity, simplicity and convenience in operation and controllable cost, and provides technical support for environmental risk assessment.

Methods and compositions for reducing silver stains on teeth treated using silver diamine fluoride are provided. The methods for removing stains comprise the steps of etching a stained area, the application of sodium hypochlorite, the application of hydrogen peroxide and the application of a remineralization solution to the stained area.

The present invention provides a new method for discovering receptors mediating tau endocytosis by modifying biotin on microtubule-associated protein tau. The present invention discovers that tau protein can be taken up by mouse hippocampal neuronal cells HT22. After silver staining following StreptAvidin-Biotin enrichment, differential bands are observed. Further, differential protein candidates are identified by mass spectrometry. The research results of the present invention provide an effective method for discovering receptor proteins mediating tau diffusion.

The invention belongs to the field of molecular biology, and discloses a method and a primer group for identifying interspecific distant hybrid filial generation of lycoris radiate and hippeastrum rutilum and application of the method and the primer group. A far-source specific primer group capable of identifying the hybrid offspring of the lycoris radiata and the hippeastrum hippeastrum is screened out, aiming at classification and identification of plants of various types and numbers, accurate identification of the interspecific hybrid offspring of the lycoris radiata and the hippeastrum hippeastrum can be conveniently and quickly realized by adopting the primer group, and both the speed and the effect are better; fluorescence labeling is applied to an SSR molecular labeling system, band information is obtained through the capillary electrophoresis technology, an existing SSR technical system is optimized and improved, and compared with silver staining detection and agarose condensation detection, automatic fluorescence detection has the advantages of being high in throughput and automatic in operation.

The present invention provides a dCAPS marker primer for identifying the color of tomato stigma, comprising a dCAPS upstream primer and a dCAPS downstream primer, and the nucleotide sequence is shown in SEQ ID NO: 1-2. A method for identifying the color of tomato stigma using the marker primer is also provided, wherein the dCAPS upstream primer and the dCAPS downstream primer are used to perform a PCR amplification reaction on the genomic DNA of the extracted sample to be tested, to obtain a PCR product, and then the PCR product is digested by endonuclease BseXI after 1.5% agarose gel electrophoresis detection, and the digested product is subjected to 12% polyacrylamide gel electrophoresis, and the digestion detection result is observed by silver staining and development; if the digestion detection result is a single band of 105bp, it is a yellow stigma tomato plant; if the digestion detection result is two bands of 68bp and 37bp, it is a homozygous green stigma tomato plant; if the digestion detection result is three bands of 105bp, 68bp and 37bp, it is a heterozygous green stigma tomato plant. The present invention provides a new basis for the genetic evaluation of tomato appearance morphology germplasm resources.