The invention discloses a
molecular identification method used for
siniperca chuatsi, siniperca scherzeri and a
hybrid f1 of the
siniperca chuatsi and the siniperca scherzeri. The
molecular identification method used for the
siniperca chuatsi, the siniperca scherzeri and the
hybrid f1 of the siniperca chuatsi and the siniperca scherzeri comprises the following steps: 1) extracting a
genome DNA of a to-be-detected mandarin fish (the siniperca chuatsi, the siniperca scherzeri and the
hybrid f1 of the siniperca chuatsi and the siniperca scherzeri) sample; 2) with the extracted
genome DNA of the to-be-detected sample as a template, amplifying
a DNA target fragment by adopting
polymerase chain reaction (PCR), wherein a pair of
microsatellite primer sequences used in the PCR are as follows: a
forward primer sequence, namely 5'ATGTAACCGTAAGTGACCTC3', and a
reverse primer sequence, namely 5'TGTTGTTCAGACGATGACGA3'; 3) carrying out electrophoretic separation on the PCR product by adopting 8% non-denatured
polyacrylamide gel, then carrying out silver
staining, and photographing to
record an
electrophoresis result; and 4) comparing with a map of a standard
digestion fragment, reading out the position of a target stripe of the to-be-detected mandarin fish sample, and respectively identifying the to-be-detected mandarin fish sample to be the siniperca chuatsi, the siniperca scherzeri or the hybrid f1 of the siniperca chuatsi and the siniperca scherzeri. The
molecular identification method used for the siniperca chuatsi, the siniperca scherzeri and the hybrid f1 of the siniperca chuatsi and the siniperca scherzeri is easy and simple to operate, the defects of morphological identification are made up, and the problems that a hybrid mandarin fish (the hybrid f1 of the siniperca chuatsi and the siniperca scherzeri) is difficult to identify and varieties are miscellaneous during
aquaculture are solved.