Method for rapidly screening bian chicken weight gain degree by molecular marking

A technology of molecular markers and body weight, applied in biochemical equipment and methods, microbial measurement/inspection, etc., can solve problems such as inability to separate and locate, ignorance of location and transmission rules, ignorance of genetic background of quantitative traits, etc.
CN102321750AInactive Publication Date: 2012-01-18YANGZHOU UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
YANGZHOU UNIV
Publication Date
2012-01-18
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to the field of poultry breeding, and particularly relates to a method for rapidly screening bian chicken weight gain degree by DNA molecular marking. The method comprises the following steps: extracting chicken genome DNA of a sample to be detected, performing specific primer amplification to obtain a MSTN gene target fragment, performing restriction endonuclease BbvI digestion of the target fragment, performing gel electrophoresis and silver staining for color development to obtain a DNA restriction enzyme map, determining the speed of weight gain of the sample to be detected based on the strip quantity and size in the map. The detection method adopted by the invention is simple and rapid, is not affected by external environment, can be used for molecular genetic marking of bian chicken weight selection, and can be used for marker assisted selection.
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Description

technical field

[0001] The invention relates to the field of poultry breeding, in particular to a method for screening the body weight growth degree of side chickens with DNA molecular markers. Background technique

[0002] Traditional quantitative genetics’ understanding of the genetic mechanism of quantitative traits is based on the abstract assumption of micro-effect polygenes, and treats all genes that affect a quantitative trait as a whole, regardless of how each gene works . This hypothesis has played an important role in many years of animal and plant breeding and promoted the progress of animal and plant breeding. It was a very good foundation under the theoretical and technical conditions at that time. However, this hypothesis does not understand the genetic background of quantitative traits, the location of genes controlling quantitative traits on chromosomes and their transmission rules, and cannot make accurate estimates of their effects, let alone isolate and l...

Claims

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