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Method for rapidly screening bian chicken weight gain degree by molecular marking

A technology of molecular markers and body weight, applied in biochemical equipment and methods, microbial measurement/inspection, etc., can solve problems such as inability to separate and locate, ignorance of location and transmission rules, ignorance of genetic background of quantitative traits, etc.

Inactive Publication Date: 2012-01-18
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this hypothesis does not understand the genetic background of quantitative traits, the location of genes controlling quantitative traits on chromosomes and their transmission rules, and cannot make accurate estimates of their effects, let alone isolate and locate such genes at the molecular level

Method used

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  • Method for rapidly screening bian chicken weight gain degree by molecular marking
  • Method for rapidly screening bian chicken weight gain degree by molecular marking
  • Method for rapidly screening bian chicken weight gain degree by molecular marking

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] 1. Extraction of genomic DNA to be tested

[0014] 1.1 Blood collection

[0015] The hens of the first generation (137) and second generation (117) were collected from the Institute of Animal Husbandry and Veterinary Medicine, Shanxi Academy of Agricultural Sciences, about 0.5 mL of blood samples were collected from the wing vein, anticoagulated with sodium heparin, and brought back to the laboratory in an ice box , stored at -20°C.

[0016] 1.2 Digestion of protein and RNA

[0017] Take 30 μL of anticoagulated blood, put it into a 1.5 mL centrifuge tube, add 0.3 mL TE, 0.3 ml lysate, 10 μL RNaseA enzyme (2.5 mg / mL), 8 μL proteinase K (20 mg / mL), mix well by inversion, Overnight at 55°C to fully digest protein and RNA.

[0018] Organic solvent extraction of DNA

[0019] Add 0.6 mL Tris saturated phenol, invert and mix for 20 min, centrifuge at 12000 rpm for 10 min; pipette the supernatant into another clean 1.5 mL centrifuge tube, add 0.6 mL phenol / chloroform / isoam...

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Abstract

The invention relates to the field of poultry breeding, and particularly relates to a method for rapidly screening bian chicken weight gain degree by DNA molecular marking. The method comprises the following steps: extracting chicken genome DNA of a sample to be detected, performing specific primer amplification to obtain a MSTN gene target fragment, performing restriction endonuclease BbvI digestion of the target fragment, performing gel electrophoresis and silver staining for color development to obtain a DNA restriction enzyme map, determining the speed of weight gain of the sample to be detected based on the strip quantity and size in the map. The detection method adopted by the invention is simple and rapid, is not affected by external environment, can be used for molecular genetic marking of bian chicken weight selection, and can be used for marker assisted selection.

Description

technical field [0001] The invention relates to the field of poultry breeding, in particular to a method for screening the body weight growth degree of side chickens with DNA molecular markers. Background technique [0002] Traditional quantitative genetics’ understanding of the genetic mechanism of quantitative traits is based on the abstract assumption of micro-effect polygenes, and treats all genes that affect a quantitative trait as a whole, regardless of how each gene works . This hypothesis has played an important role in many years of animal and plant breeding and promoted the progress of animal and plant breeding. It was a very good foundation under the theoretical and technical conditions at that time. However, this hypothesis does not understand the genetic background of quantitative traits, the location of genes controlling quantitative traits on chromosomes and their transmission rules, and cannot make accurate estimates of their effects, let alone isolate and l...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 张跟喜丁馥香王金玉谢恺舟戴国俊张李俊张丽赵秀华
Owner YANGZHOU UNIV
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