8120 results about "Genetics" patented technology

The invention provides methods, systems, and computer readable medium for detecting ploidy of chromosome segments or entire chromosomes, for detecting single nucleotide variants and for detecting both ploidy of chromosome segments and single nucleotide variants. In some aspects, the invention provides methods, systems, and computer readable medium for detecting cancer or a chromosomal abnormality in a gestating fetus.

The invention provides methods, systems, and computer readable medium for detecting ploidy of chromosome segments or entire chromosomes, for detecting single nucleotide variants and for detecting both ploidy of chromosome segments and single nucleotide variants. In some aspects, the invention provides methods, systems, and computer readable medium for detecting cancer or a chromosomal abnormality in a gestating fetus.

The invention provides methods, systems, and computer readable medium for detecting ploidy of chromosome segments or entire chromosomes, for detecting single nucleotide variants and for detecting both ploidy of chromosome segments and single nucleotide variants. In some aspects, the invention provides methods, systems, and computer readable medium for detecting cancer or a chromosomal abnormality in a gestating fetus.

Provided herein are methods of identifying genetic variants in biological samples. In particular, the present disclosure uses spatial transcriptomic templated ligation methods and compositions to identify the presence or absence, and the location of genetic variants in the transcriptome or genome of a biological sample.

The invention provides methods, systems, and computer readable medium for detecting ploidy of chromosome segments or entire chromosomes, for detecting single nucleotide variants and for detecting both ploidy of chromosome segments and single nucleotide variants. In some aspects, the invention provides methods, systems, and computer readable medium for detecting cancer or a chromosomal abnormality in a gestating fetus.

Compositions and methods for editing deleterious mutations associated with Alpha-1 Antitrypsin Deficiency (A1AD). In particular embodiments, the invention provides methods for treating A1AD using a modified adenosine base editor with improved on-target editing and decreased off-target editing to correct mutations associated with A1AD.

Methods for making synthetic gene clusters are described.

The invention discloses primers, a kit and a method for identifying whether a sugarcane material contains a saccharum arundinaceum chromosome 7 or not. According to the method, whether the sugarcane material contains the saccharum arundinaceum chromosome 7 or not can be stably detected by utilizing the saccharum arundinaceum specific sequence PCR primer TaChr07-F / R (the sequence is shown as SEQ ID NO.1-2) through a conventional common PCR reaction program, and the method has the advantages of high efficiency, good stability, low cost, simplicity and convenience in operation and good generalization performance, and can be applied to improvement of sugarcane germplasm resources or sugarcane breeding.

The invention belongs to the technical field of genetic breeding, and particularly relates to a molecular marker influencing sheep weight traits and application thereof, and the molecular marker comprises a site I or a site II. The nucleotide sequence of the molecular marker containing the site I is as shown in SEQ ID NO.1, and mutation from T to C occurs at the 101bp position in the SEQ ID NO.1; the nucleotide sequence of the molecular marker containing the site 2 is as shown in SEQ ID NO.2, and mutation from A to G occurs at the 101bp position in the SEQ ID NO.2. According to the molecular markers influencing the sheep weight traits, the marker I and the marker II can be used for identifying the sheep weight traits, and the offspring weight can be increased by selecting TT or AA genotype individuals as male parents or female parents.

The invention belongs to the technical field of molecular biology, and particularly relates to a molecular marker related to the number of branches of medicago sativa and application of the molecular marker. The invention solves the technical problems that the traditional new variety breeding of alfalfa with a large number of branches is single plant selection according to the number of branches of breeding offspring, and the method is time-consuming, labor-consuming and low in accuracy. The technical scheme of the invention is a molecular marker related to the number of branches of medicago sativa. The specific information is as follows: chromosome: chr2; the position is 57649197; sNP (Single Nucleotide Polymorphism) typing: C / A. The invention also designs a primer combination aiming at the molecular marker, and the primer combination can be used for directly and specifically distinguishing and detecting A or C basic groups of SNP mutation sites.

The invention relates to the technical field of bee breeding, in particular to an SNP (Single Nucleotide Polymorphism) molecular marker and application thereof in improving bee germplasm resources. The SNP molecular marker is based on a genome version PRJNA738447, the SNP molecular marker is located at the 3613559th site of the 11 # chromosome of the bee, and the polymorphism is C / T. The SNP molecular marker provided by the invention is closely linked with the length of the tarsal node of the bee, and the detection of the length of the tarsal node of the bee can be realized through a genotype detection result. The SNP molecular marker provided by the invention can be used for breeding transgenic bees and improving bee germplasm resources, and has important application value in the field of bee breeding.

The invention discloses a litopenaeus vannamei body length growth trait associated SNP molecular marker, a detection primer and application thereof. The sequence of the SNP molecular marker is as shown in SEQ ID NO.1, the SNP site is located at the 282 site from the 5'end of the sequence as shown in SEQ ID NO.1, and the polymorphism of the SNP molecular marker is T / C type. The SNP locus can be used for body length screening of litopenaeus vannamei in litopenaeus vannamei assisted breeding, and a primer designed according to the sequence of SEQ ID NO.1 or a kit prepared from the primer can be used for detecting the SNP locus. The SNP molecular marker Chr3711743678 provided by the invention can be used for assisting in selecting rapid growth parents, and the CC genotype in the SNP molecular marker is used for selecting the parents, so that the growth speed of breeding populations can be increased, an innovative tool is provided for molecular breeding of litopenaeus vannamei, the breeding period is shortened, and industrial development and efficiency improvement are assisted.

The embodiment of the invention provides a rice blast disease grade intelligent evaluation and disease resistance preliminary screening method, system and equipment, and the method comprises the steps: S1, collecting image data of wild rice leaves infected with rice blast, carrying out the instance segmentation of the image data through an improved YOLOv8-Seg segmentation model, obtaining a segmentation result, and carrying out the segmentation of the image data through an improved YOLOv8-Seg segmentation model; the improvement measures comprise a GhostHierachicalNet backbone network, a CAHSFPN neck network and an LSDES Head segmentation head, and the LSDES Head segmentation head is connected with the CAHSFPN neck network and the LSDES Head segmentation head; s2, according to the segmentation result, counting the number and proportion of pixels of diseased leaves and disease areas, and obtaining a disease grade evaluation result in combination with disease resistance identification technical regulations; and S3, screening wild rice materials with strong disease resistance based on the disease grade evaluation results which are repeated for multiple times. The method can effectively improve the accuracy and efficiency of crop disease evaluation, and provides support for large-scale screening of disease resistance of wild rice, thereby promoting the development of rice blast-resistant breeding work.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker site related to wheat cold resistance and application of the SNP molecular marker site. The molecular site is located at the 101st basic group from the 5'tail end of a sequence as shown in SEQ ID NO.1, and the polymorphism is C / T. The molecular marker related to wheat cold resistance in the wintering period provided by the invention can be used for detecting whether a wheat variety or strain contains a cold-resistant site or not, and has very high value for cold-resistant breeding of wheat, and meanwhile, the developed KASP molecular marker can be used for greatly accelerating the breeding process of the wheat cold-resistant variety.

The invention provides a specific single nucleotide polymorphism (SNP) molecular marker for sex identification of cobia and application of the specific single nucleotide polymorphism (SNP) molecular marker. Based on whole genome high-throughput sequencing data of male and female cobia, after deep comparative analysis, the specific molecular marker related to the sex of cobia is screened for the first time, and the primer pair designed for the marker can be used independently or used in a combined mode and is high in flexibility. The method has relatively high judgment accuracy, so that the sex of cobia can be quickly and accurately identified, and the method has a very good application prospect.

The invention provides a molecular marker related to salt-tolerant rapid growth traits of grass carps and application of the molecular marker, and belongs to the technical field of molecular markers. According to the invention, a group of molecular markers related to salt-tolerant and rapid growth traits of grass carp are obtained through GWAS analysis and screening. The invention also develops a group of amplification primers based on the molecular marker, the amplification primers can realize screening and / or identification of salt-tolerant fast-growing grass carp germplasm, and the more the enriched dominant genotypes are, the higher the growth trait index is. Compared with the prior art, the molecular marker provided by the invention can be used for accurately and efficiently identifying high-quality grass carp individuals with salt-tolerant and fast-growing characters, and the salt-tolerant breeding progress of grass carp is accelerated.

The invention relates to the technical field of molecular biology and molecular markers, in particular to an SNP (Single Nucleotide Polymorphism) molecular marker located on a porcine chromosome 14 and related to porcine reproductive traits and application. The SNP site of the SNP molecular marker located on the No.14 chromosome of the pig and related to the reproductive traits of the pig corresponds to the Cgt at the 105043272th site on the No.14 chromosome of the international pig reference genome version 11.1; t mutation is carried out, and the polymorphism of the basic group at the site affects the porcine reproductive traits; wherein the reproductive performance of pigs with CC and TC genotypes is superior to that of pigs with TT genotypes. By optimizing the dominant alleles of the SNP molecular marker, the frequency of the dominant alleles can be increased generation by generation, the reproductive performance of pigs is improved, and the genetic improvement progress of the pigs is accelerated, so that the economic benefit of pig breeding is effectively improved.

The invention discloses a molecular marker for auxiliary screening of anti-freezing wheat germplasm and application of the molecular marker, and relates to the technical field of biology, in particular to a method for screening or auxiliary screening of wheat with different freezing resistance, which comprises the following steps: detecting whether the genotype of wheat to be detected is qFDG-7A. 3a or qFDG-7A. 3b, and the freezing resistance gt of the wheat with the genotype qFDG-7A. 3a; the freezing resistance of the wheat with the genotype qFDG-7A. 3b; the wheat of which the genotype is qFDG-7A. 3a is wheat of which the genotype is AA homozygous based on an A129C SNP (Single Nucleotide Polymorphism) site; the wheat of which the genotype is qFDG-7A. 3b is wheat of which the genotype is CC homozygous based on an A129C SNP (Single Nucleotide Polymorphism) site; the A129C SNP site is the 129th nucleotide from the 5'tail end of SEQ ID NO: 1 in a wheat genome. The molecular marker has important theoretical significance and economic value for molecular marker-assisted selection of wheat germplasm or breeding progeny materials with high anti-freezing capacity.

The invention belongs to the technical field of molecular biotechnology and molecular markers, and particularly relates to an SNP (Single Nucleotide Polymorphism) molecular marker located on a chromosome 2 and related to abnormal delivery traits of sows and application of the SNP molecular marker. An SNP site of the SNP molecular marker corresponds to Cgt at the 23330829bp position on a chromosome 2 of an international pig reference genome version 11.1; and the polymorphism of the basic group at the site affects the abnormal delivery character of the sow. The method solves the problem of genetic improvement of abnormal delivery of sows by means of molecular breeding, and can increase the frequency of dominant alleles generation by generation, reduce the occurrence rate of abnormal delivery of sows and improve the reproductive performance of sows by optimizing the dominant alleles of variation sites. The pig genetic improvement progress is accelerated, so that the economic benefit of breeding pig breeding is effectively improved.

The invention discloses a wheat thousand seed weight related SNP-C1216T and application thereof, the SNP site corresponds to the 1216th basic group from the 5'terminal of the sequence shown in SEQ ID NO.1, and when the site is C / C homozygous, the corresponding genotype is A; when the locus is T / T homozygous, the corresponding genotype is B; the thousand seed weight of the wheat with the homozygous genotype A is greater than or candidate to be greater than that of the wheat with the homozygous genotype B. The SNP has high effectiveness and potential application value, wheat with high thousand seed weight can be found by detecting the SNP, and the SNP has important value in research or application of cultivation of high-yield wheat varieties.

The invention discloses a cowpea 5K SNP (Single Nucleotide Polymorphism) liquid chip and application thereof in variety identification, and belongs to the technical field of biology. The liquid chip provided by the invention comprises a set of nucleotide probes for detecting 5847 SNP sites of cowpea, and can be widely applied to different scenes of germplasm resource identification, genetic diversity evaluation, variety identification, genetic relationship identification, purification and rejuvenation of local varieties and the like of different cowpea varieties; an effective scientific basis is provided for cowpea variety identification, the variety identification and evaluation efficiency is improved, and the method has a remarkable application value.

Disclosed herein are certain chemical compounds that inhibit Werner Syndrome ATP dependent helicase enzyme (WRN) activity, in particular inhibit WRN helicase domain activity and are therefore useful in treating cancers treatable by inhibition of WRN, including cancers characterized by microsatellite instability (MSI) and / or defective DNA mismatch repair system (dMMR). Also, disclosed are pharmaceutical compositions comprising such compounds, methods of using such compounds, and methods making the same.

The invention discloses a macrobrachium rosenbergii iridovirus disease resistance-related SNP (Single Nucleotide Polymorphism) site, a site combination and application thereof, and belongs to the field of aquatic organism breeding. According to the invention, SNP loci related to resistance of the macrobrachium rosenbergii iridovirus disease are successfully screened through whole genome association analysis, and the SNP loci are respectively located at a 9574545th base of a chromosome 10 of the macrobrachium rosenbergii and a 26491028th base of a chromosome 14 of the macrobrachium rosenbergii. Aiming at the two SNP sites, an SNP site combination related to the resistance of the macrobrachium rosenbergii iridovirus disease is further developed, and macrobrachium rosenbergii iridovirus disease resistant individuals can be screened by detecting the SNP site combination. Therefore, the SNP molecular marker provided by the invention can be used for macrobrachium rosenbergii molecular marker assisted breeding, accelerates disease-resistant macrobrachium rosenbergii variety breeding, and provides a reference basis for disease-resistant breeding work of macrobrachium rosenbergii.

A novel soybean variety, designated 5PNMK70 is provided. Also provided are the seeds of soybean variety 5PNMK70, cells from soybean variety 5PNMK70, plants of soybean 5PNMK70, and plant parts of soybean variety 5PNMK70. Methods provided include producing a soybean plant by crossing soybean variety 5PNMK70 with another soybean plant, methods for introgressing a transgenic trait, a mutant trait, and / or a native trait into soybean variety 5PNMK70, methods for producing other soybean varieties or plant parts derived from soybean variety 5PNMK70, and methods of characterizing soybean variety 5PNMK70. Soybean seed, cells, plants, germplasm, breeding lines, varieties, and plant parts produced by these methods and / or derived from soybean variety 5PNMK70 are further provided.

The invention discloses SNP-C682T related to the number of grains per ear of wheat and application of the SNP-C682T. An SNP site corresponds to a 682th basic group from the 5'tail end of a sequence shown in SEQ ID NO.1, and when the site is C / C homozygous, the corresponding genotype is A; when the locus is T / T homozygous, the corresponding genotype is B; the grain number per ear is as follows: the wheat with the homozygous genotype A is greater than or candidate to be greater than the wheat with the homozygous genotype B. The SNP has high effectiveness and potential application value, the wheat with the large grain number per ear can be found by detecting the SNP, and the SNP has important value in research or application of cultivation of high-yield wheat varieties.

The invention discloses a tomato leaf disease detection method based on an SSP-DETR model, and the method is realized through the SSP-DETR model, and comprises the steps: obtaining a tomato leaf disease image data set, and carrying out the classification; inputting the tomato leaf disease image into a StarNet network module for feature extraction, and outputting feature maps P2, P3, P4 and P5 of different dimensions; inputting P5 into an attention feature extraction module, and outputting F5; p2, P3, P4 and F5 are input into a multi-scale fusion module for feature fusion; inputting the fused feature map into a Decoder decoder, and outputting a tomato leaf disease area prediction frame; acquiring a tomato leaf disease area prediction frame and disease area classification in the corresponding tomato leaf disease image, and performing back propagation on the optimization model by using a loss function; and inputting the tomato leaf disease image into the optimized model to obtain a disease detection result. According to the method, the tomato leaf diseases can be comprehensively detected, the detection precision is high, and the robustness is good.

A method of making a barcoded surface includes synthesizing a plurality of arrays arranged in rows and columns. Each array can include a plurality of discrete reaction sites with a first oligonucleotide containing a first unique array barcode sequence. The first unique array barcode sequences of the first oligonucleotides corresponds to a mapped discrete reaction site location within the array. A second oligonucleotide can be coupled to the first oligonucleotide that contains a second unique barcode sequence. The first unique barcode sequences of the second oligonucleotides corresponds to a mapped first sub-group location within the plurality of arrays.

A wheat variety designated KS12D03-1 is disclosed, including plants and seeds thereof. KS12D03-1 is a durum wheat variety with a winter growth habit making it suitable for production in western Kansas and the High Plains. Methods for producing a wheat plant produced by crossing the variety KS12D03-1 with another wheat plant, and hybrid wheat seeds and plants produced by crossing the variety KS12D03-1 with another wheat line or plant, and the creation of variants by backcrossing, mutagenesis or transformation of variety KS12D03-1 are disclosed. Methods for producing other wheat varieties or breeding lines derived from wheat variety KS12D03-1 and to wheat varieties or breeding lines produced by those methods are also provided.

Provided are a double-stranded oligonucleotide agent and uses. The double-stranded oligonucleotide agent comprises a specific cleavage site for an endonuclease, wherein the cleaved double-stranded oligonucleotide agent is capable of inhibiting expression of the target gene via RNA interference (RNAi).

The invention relates to a composition for identifying epinephelus fuscoguttatus, epinephelus coioides and hybrid offspring and application, and belongs to the field of molecular biology, the composition comprises a pair of primers and a group of probes, and the sequences are shown as SEQ ID NO.1-4. The invention further provides a kit for identifying the epinephelus fuscoguttatus, the epinephelus coioides and the hybrid offspring thereof, and the kit is used for identifying the epinephelus fuscoguttatus and the hybrid offspring of the epinephelus coioides and the hybrid offspring of the epinephelus coioides and the hybrid offspring of the epinephelus coioides. The kit comprises the composition disclosed by the invention. The composition can be used for rapidly identifying epinephelus fuscoguttatus, epinephelus coioides and hybrid filial generation gold and tiger hybrid spots of the epinephelus coioides, the epinephelus coioides and the hybrid filial generation gold and tiger hybrid spots of the epinephelus coioides and the epinephelus coioides and the hybrid filial generation gold and tiger hybrid spots of the epinephelus