5857 results about "Genetics" patented technology

Provided herein are methods of identifying genetic variants in biological samples. In particular, the present disclosure uses spatial transcriptomic templated ligation methods and compositions to identify the presence or absence, and the location of genetic variants in the transcriptome or genome of a biological sample.

The invention provides methods, systems, and computer readable medium for detecting ploidy of chromosome segments or entire chromosomes, for detecting single nucleotide variants and for detecting both ploidy of chromosome segments and single nucleotide variants. In some aspects, the invention provides methods, systems, and computer readable medium for detecting cancer or a chromosomal abnormality in a gestating fetus.

The invention belongs to the technical field of genetic breeding, and particularly relates to a molecular marker influencing sheep weight traits and application thereof, and the molecular marker comprises a site I or a site II. The nucleotide sequence of the molecular marker containing the site I is as shown in SEQ ID NO.1, and mutation from T to C occurs at the 101bp position in the SEQ ID NO.1; the nucleotide sequence of the molecular marker containing the site 2 is as shown in SEQ ID NO.2, and mutation from A to G occurs at the 101bp position in the SEQ ID NO.2. According to the molecular markers influencing the sheep weight traits, the marker I and the marker II can be used for identifying the sheep weight traits, and the offspring weight can be increased by selecting TT or AA genotype individuals as male parents or female parents.

The invention belongs to the technical field of molecular biology, and particularly relates to a molecular marker related to the number of branches of medicago sativa and application of the molecular marker. The invention solves the technical problems that the traditional new variety breeding of alfalfa with a large number of branches is single plant selection according to the number of branches of breeding offspring, and the method is time-consuming, labor-consuming and low in accuracy. The technical scheme of the invention is a molecular marker related to the number of branches of medicago sativa. The specific information is as follows: chromosome: chr2; the position is 57649197; sNP (Single Nucleotide Polymorphism) typing: C / A. The invention also designs a primer combination aiming at the molecular marker, and the primer combination can be used for directly and specifically distinguishing and detecting A or C basic groups of SNP mutation sites.

The invention discloses a litopenaeus vannamei body length growth trait associated SNP molecular marker, a detection primer and application thereof. The sequence of the SNP molecular marker is as shown in SEQ ID NO.1, the SNP site is located at the 282 site from the 5'end of the sequence as shown in SEQ ID NO.1, and the polymorphism of the SNP molecular marker is T / C type. The SNP locus can be used for body length screening of litopenaeus vannamei in litopenaeus vannamei assisted breeding, and a primer designed according to the sequence of SEQ ID NO.1 or a kit prepared from the primer can be used for detecting the SNP locus. The SNP molecular marker Chr3711743678 provided by the invention can be used for assisting in selecting rapid growth parents, and the CC genotype in the SNP molecular marker is used for selecting the parents, so that the growth speed of breeding populations can be increased, an innovative tool is provided for molecular breeding of litopenaeus vannamei, the breeding period is shortened, and industrial development and efficiency improvement are assisted.

The embodiment of the invention provides a rice blast disease grade intelligent evaluation and disease resistance preliminary screening method, system and equipment, and the method comprises the steps: S1, collecting image data of wild rice leaves infected with rice blast, carrying out the instance segmentation of the image data through an improved YOLOv8-Seg segmentation model, obtaining a segmentation result, and carrying out the segmentation of the image data through an improved YOLOv8-Seg segmentation model; the improvement measures comprise a GhostHierachicalNet backbone network, a CAHSFPN neck network and an LSDES Head segmentation head, and the LSDES Head segmentation head is connected with the CAHSFPN neck network and the LSDES Head segmentation head; s2, according to the segmentation result, counting the number and proportion of pixels of diseased leaves and disease areas, and obtaining a disease grade evaluation result in combination with disease resistance identification technical regulations; and S3, screening wild rice materials with strong disease resistance based on the disease grade evaluation results which are repeated for multiple times. The method can effectively improve the accuracy and efficiency of crop disease evaluation, and provides support for large-scale screening of disease resistance of wild rice, thereby promoting the development of rice blast-resistant breeding work.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker site related to wheat cold resistance and application of the SNP molecular marker site. The molecular site is located at the 101st basic group from the 5'tail end of a sequence as shown in SEQ ID NO.1, and the polymorphism is C / T. The molecular marker related to wheat cold resistance in the wintering period provided by the invention can be used for detecting whether a wheat variety or strain contains a cold-resistant site or not, and has very high value for cold-resistant breeding of wheat, and meanwhile, the developed KASP molecular marker can be used for greatly accelerating the breeding process of the wheat cold-resistant variety.

The invention provides a specific single nucleotide polymorphism (SNP) molecular marker for sex identification of cobia and application of the specific single nucleotide polymorphism (SNP) molecular marker. Based on whole genome high-throughput sequencing data of male and female cobia, after deep comparative analysis, the specific molecular marker related to the sex of cobia is screened for the first time, and the primer pair designed for the marker can be used independently or used in a combined mode and is high in flexibility. The method has relatively high judgment accuracy, so that the sex of cobia can be quickly and accurately identified, and the method has a very good application prospect.

The invention relates to the technical field of molecular biology and molecular markers, in particular to an SNP (Single Nucleotide Polymorphism) molecular marker located on a porcine chromosome 14 and related to porcine reproductive traits and application. The SNP site of the SNP molecular marker located on the No.14 chromosome of the pig and related to the reproductive traits of the pig corresponds to the Cgt at the 105043272th site on the No.14 chromosome of the international pig reference genome version 11.1; t mutation is carried out, and the polymorphism of the basic group at the site affects the porcine reproductive traits; wherein the reproductive performance of pigs with CC and TC genotypes is superior to that of pigs with TT genotypes. By optimizing the dominant alleles of the SNP molecular marker, the frequency of the dominant alleles can be increased generation by generation, the reproductive performance of pigs is improved, and the genetic improvement progress of the pigs is accelerated, so that the economic benefit of pig breeding is effectively improved.

The invention discloses a molecular marker for auxiliary screening of anti-freezing wheat germplasm and application of the molecular marker, and relates to the technical field of biology, in particular to a method for screening or auxiliary screening of wheat with different freezing resistance, which comprises the following steps: detecting whether the genotype of wheat to be detected is qFDG-7A. 3a or qFDG-7A. 3b, and the freezing resistance gt of the wheat with the genotype qFDG-7A. 3a; the freezing resistance of the wheat with the genotype qFDG-7A. 3b; the wheat of which the genotype is qFDG-7A. 3a is wheat of which the genotype is AA homozygous based on an A129C SNP (Single Nucleotide Polymorphism) site; the wheat of which the genotype is qFDG-7A. 3b is wheat of which the genotype is CC homozygous based on an A129C SNP (Single Nucleotide Polymorphism) site; the A129C SNP site is the 129th nucleotide from the 5'tail end of SEQ ID NO: 1 in a wheat genome. The molecular marker has important theoretical significance and economic value for molecular marker-assisted selection of wheat germplasm or breeding progeny materials with high anti-freezing capacity.

The invention discloses a wheat thousand seed weight related SNP-C1216T and application thereof, the SNP site corresponds to the 1216th basic group from the 5'terminal of the sequence shown in SEQ ID NO.1, and when the site is C / C homozygous, the corresponding genotype is A; when the locus is T / T homozygous, the corresponding genotype is B; the thousand seed weight of the wheat with the homozygous genotype A is greater than or candidate to be greater than that of the wheat with the homozygous genotype B. The SNP has high effectiveness and potential application value, wheat with high thousand seed weight can be found by detecting the SNP, and the SNP has important value in research or application of cultivation of high-yield wheat varieties.

The invention discloses a cowpea 5K SNP (Single Nucleotide Polymorphism) liquid chip and application thereof in variety identification, and belongs to the technical field of biology. The liquid chip provided by the invention comprises a set of nucleotide probes for detecting 5847 SNP sites of cowpea, and can be widely applied to different scenes of germplasm resource identification, genetic diversity evaluation, variety identification, genetic relationship identification, purification and rejuvenation of local varieties and the like of different cowpea varieties; an effective scientific basis is provided for cowpea variety identification, the variety identification and evaluation efficiency is improved, and the method has a remarkable application value.

A novel soybean variety, designated 5PNMK70 is provided. Also provided are the seeds of soybean variety 5PNMK70, cells from soybean variety 5PNMK70, plants of soybean 5PNMK70, and plant parts of soybean variety 5PNMK70. Methods provided include producing a soybean plant by crossing soybean variety 5PNMK70 with another soybean plant, methods for introgressing a transgenic trait, a mutant trait, and / or a native trait into soybean variety 5PNMK70, methods for producing other soybean varieties or plant parts derived from soybean variety 5PNMK70, and methods of characterizing soybean variety 5PNMK70. Soybean seed, cells, plants, germplasm, breeding lines, varieties, and plant parts produced by these methods and / or derived from soybean variety 5PNMK70 are further provided.

The invention discloses a tomato leaf disease detection method based on an SSP-DETR model, and the method is realized through the SSP-DETR model, and comprises the steps: obtaining a tomato leaf disease image data set, and carrying out the classification; inputting the tomato leaf disease image into a StarNet network module for feature extraction, and outputting feature maps P2, P3, P4 and P5 of different dimensions; inputting P5 into an attention feature extraction module, and outputting F5; p2, P3, P4 and F5 are input into a multi-scale fusion module for feature fusion; inputting the fused feature map into a Decoder decoder, and outputting a tomato leaf disease area prediction frame; acquiring a tomato leaf disease area prediction frame and disease area classification in the corresponding tomato leaf disease image, and performing back propagation on the optimization model by using a loss function; and inputting the tomato leaf disease image into the optimized model to obtain a disease detection result. According to the method, the tomato leaf diseases can be comprehensively detected, the detection precision is high, and the robustness is good.

A method of making a barcoded surface includes synthesizing a plurality of arrays arranged in rows and columns. Each array can include a plurality of discrete reaction sites with a first oligonucleotide containing a first unique array barcode sequence. The first unique array barcode sequences of the first oligonucleotides corresponds to a mapped discrete reaction site location within the array. A second oligonucleotide can be coupled to the first oligonucleotide that contains a second unique barcode sequence. The first unique barcode sequences of the second oligonucleotides corresponds to a mapped first sub-group location within the plurality of arrays.

The invention relates to a composition for identifying epinephelus fuscoguttatus, epinephelus coioides and hybrid offspring and application, and belongs to the field of molecular biology, the composition comprises a pair of primers and a group of probes, and the sequences are shown as SEQ ID NO.1-4. The invention further provides a kit for identifying the epinephelus fuscoguttatus, the epinephelus coioides and the hybrid offspring thereof, and the kit is used for identifying the epinephelus fuscoguttatus and the hybrid offspring of the epinephelus coioides and the hybrid offspring of the epinephelus coioides and the hybrid offspring of the epinephelus coioides. The kit comprises the composition disclosed by the invention. The composition can be used for rapidly identifying epinephelus fuscoguttatus, epinephelus coioides and hybrid filial generation gold and tiger hybrid spots of the epinephelus coioides, the epinephelus coioides and the hybrid filial generation gold and tiger hybrid spots of the epinephelus coioides and the epinephelus coioides and the hybrid filial generation gold and tiger hybrid spots of the epinephelus

A method for sentiment based enhancement of investor relations communication. The method includes: receiving an enhancement request including an original current earnings call transcript; processing, of the original current earnings call transcript, a plurality of original sentiment-pertinent transcript sentences using language models to respectively obtain a plurality of transcript sentence ranks; identifying a subset of the plurality of transcript sentence ranks each indicating a sentiment improvement; producing, from the original current earnings call transcript, a new current earnings call transcript including a set of enhanced sentiment-pertinent transcript sentences respectively mapped to the subset of the plurality of transcript sentence ranks; and providing the new current earnings call transcript in response to the enhancement request.

The invention provides an Indel molecular marker primer pair, a kit and a method for identifying Yichang orange, and belongs to the technical field of biochemistry, the Indel molecular marker primer pair comprises at least one of an InDel-2 primer pair and an InDel-3 primer pair; the sequences of the InDel-2 primer pair are as shown in SEQ ID No. 1 and SEQ ID No. 2, and the sequences of the InDel-3 primer pair are as shown in SEQ ID No. 3 and SEQ ID No. 4. The Indel molecular marker primer pair or the kit disclosed by the invention can specifically recognize the Yichang oranges, realizes efficient and accurate identification of the Yichang oranges and filial generations thereof, and has wide application prospects in the aspects of citrus germplasm resource protection, variety right and interest maintenance, breeding selection, market supervision and the like. The method for identifying the Yichang oranges can accurately identify the Yichang oranges and the filial generations of the Yichang oranges, has high specificity, economical efficiency and high efficiency, is an accurate, economical and efficient hybrid identification means, can effectively identify the variety of the Yichang oranges and the hybrids of the Yichang oranges, and provides powerful technical support for scientific research and breeding work.

A wheat variety designated PMWH111720248, the plants and seeds of wheat variety PMWH111720248, methods for producing a wheat plant produced by crossing the variety PMWH111720248 with another wheat plant, and hybrid wheat seeds and plants produced by crossing the variety PMWH111720248 with another wheat line or plant, and the creation of variants by backcrossing, mutagenesis or transformation of variety PMWH111720248 are disclosed. Methods for producing other wheat varieties or breeding lines derived from wheat variety PMWH111720248 and to wheat varieties or breeding lines produced by those methods are also provided.

The invention discloses a hemoglobin-resistant Taq DNA polymerase mutant and a construction method thereof, and relates to the field of biology, and the hemoglobin-resistant Taq DNA polymerase mutant is characterized in that a nucleotide sequence for coding the Taq DNA polymerase is shown as SEQ ID NO.1, and the mutant comprises at least one mutation or all mutations selected from S623D and E721A sites. On the basis of natural Taq DNA polymerase, the molecular structure of the Taq DNA polymerase is modified through rational design and combination with a site-specific mutagenesis biotechnology, so that the Taq DNA polymerase mutant is more suitable for PCR amplification of samples containing hemoglobin and the like than a wild type, and the result judgment accuracy of a sample containing a hemoglobin inhibitor is more facilitated.

A low-density liquid-phase chip for Gossypium hirsutum L. based on targeted capture sequencing and use thereof provided. The low-density chip includes 908 SNP loci, where among the 908 SNP loci, 329 loci are significantly related to important agronomic traits, such as fiber quality, yield, and disease resistance of Gossypium hirsutum L., 14 loci are common plant transgenic detection loci, and 565 loci are other loci. This chip is adapted for use of detection of transgenic components in Gossypium hirsutum L. varieties, resource evaluation and lineage identification, seed purity identification, and genetic improvement of major agronomic traits of Gossypium hirsutum L. varieties.

The invention discloses a silkworm disease prediction method and system based on a silkworm breeding environment, and relates to the technical field of prediction methods, the silkworm breeding environment of silkworms is determined based on environment detection of a silkworm environment area, an abnormal area of a silkworm breeding room is determined according to the silkworm breeding environment of the silkworms and a silkworm activity track, and an abnormal image of the silkworms is marked. Accurate detection of the abnormal area of the silkworm breeding room is ensured, and the accuracy of the abnormal image of the silkworm is ensured. Therefore, according to the abnormal images of the silkworms, the skin abnormal features and the posture abnormal features of the silkworms are determined, and the excrement forms of the silkworms are determined through detection along the movement tracks of the silkworms; the silkworm disease type of the silkworms is predicted based on the skin abnormal features and posture abnormal features of the silkworms and the excrement forms of the silkworms, the environment regulation and control logic of the silkworm breeding room is triggered according to the silkworm disease type of the silkworms and the silkworm breeding environment of the silkworms, accurate prediction of the silkworm disease type of the silkworms is achieved, and the silkworm breeding environment of the silkworms in the silkworm breeding room is optimized in time.

The invention includes a mutant T7 DNA ligase or a biologically active fragment thereof, which has greater activity than wild type T7 DNA ligase. The mutant T7 DNA ligase, or the biologically active fragment, has one or more substitutions differing from the wild type, as described more fully in the Summary. The preferred mutant T7 DNA ligase has at least one of the following mutations: E63K (SEQ ID NO:4), K73E (SEQ ID NO:6), K137E (SEQ ID NO:7), K174E (SEQ ID NO:9), E182K (SEQ ID NO:11), K210E (SEQ ID NO: 13), E243K (SEQ ID NO:15), D245R (SEQ ID NO: 17), E268K (SEQ ID NO:19), E272K (SEQ ID NO:21), E289K (SEQ ID NO:23), K295E (SEQ ID NO:25) and D336R (SEQ ID NO:27).

The invention belongs to the technical field of aquatic biotechnology and molecular marker-assisted breeding, and particularly relates to a cynoglossus semilaevis vibrio harveyi-resistant KASP molecular marker combination and application thereof in cynoglossus semilaevis disease-resistant breeding. The KASP molecular marker combination comprises three SNP (Single Nucleotide Polymorphism) loci which are respectively positioned at 17314099, 17325496 and 17355989 loci of the 14 # chromosome of the cynoglossus semilaevis, when the genotypes of the three SNP loci are respectively CC, AA and GG, the disease resistance of the cynoglossus semilaevis is high, and the invention also provides a primer for detecting the genotype polymorphism of the SNP loci. By utilizing the KASP molecular marker combination, the vibrio harveyi resistance genotype in a cynoglossus semilaevis breeding population can be quickly detected, and disease-resistant individuals are screened, so that the disease-resistant breeding efficiency of the cynoglossus semilaevis is greatly improved, and a new variety of the cynoglossus semilaevis with strong disease resistance can be quickly cultivated.

The invention relates to the technical field of molecular markers, in particular to KASP primers of 112 carya illinoensis varieties, an SNP molecular marker, a fingerprint spectrum and application of the KASP primers and the SNP molecular marker. By adopting the technical scheme provided by the invention, the carya illinoensis fingerprint spectrum can be drawn, is used for accurate identification of carya illinoensis varieties, and has important significance on germplasm resource identification and molecular marker breeding of carya illinoensis.

The invention discloses a KASP molecular marker for identifying soybean hundred-grain weight and application thereof, and relates to the technical field of biology and application of an SNP locus, the SNP locus takes a soybean Wm82. A2. V1 genome sequence as a reference genome, the SNP is the 16321243th SNP on a soybean 11 # chromosome, and the SNP is the 16321243th SNP on the soybean 11 # chromosome. Corresponding to the 20th basic group from the 5'terminal of the sequence shown in SEQ ID NO: 1, when the locus is CC homozygosis, the corresponding genotype is A; when the locus is TT homozygous, the corresponding genotype is B; according to the application, soybeans with different hundred-grain weights are screened or screened in an auxiliary mode, and the different hundred-grain weights of the soybeans are as follows: the homozygous soybeans with the genotype A are higher than or candidate to be higher than the homozygous soybeans with the genotype B. The molecular marker has important theoretical significance and economic value for molecular marker-assisted selection of soybean germplasm or breeding progeny materials with high hundred-grain weight.

The invention provides an expansible ring enzyme mutant and an application of the expansible ring enzyme mutant in synthesis of G-7-ADCA. A series of mutants with penicillin G ring expansion activity are obtained through ancestor enzyme sequence reconstruction, directed evolution and the like, and the mutants can directly expand the ring of the substrate penicillin G to generate G-7-ADCA. Therefore, the expansive ring enzyme mutants have important application value in industry.

The invention discloses a sheep whole genome SNP molecular marker, which comprises 50000 SNP molecular markers, the SNP molecular marker is composed of 5235 functional SNP molecular markers and 44765 SNP molecular markers, the functional SNP molecular markers mainly select variation sites related to economic traits (such as growth, slaughtering, meat quality, breeding, lamb and the like) of sheep, and the variation sites are selected from the variation sites. And the background SNP molecular marker is used for improving the typing precision and polymorphism coverage of the chip, so that the applicability of the chip in a large-scale group is ensured.

Among other things, the present disclosure provides oligonucleotides, compositions and methods thereof that can bring about specific editing of a target adenosine in a target RNA molecule. Such oligonucleotides, compositions and methods are useful to treat, prevent, or ameliorate MECP2 associated disorders, diseases and syndromes that can benefit from adenosine modification.

The invention belongs to the technical field of biology, and particularly provides a reverse transcriptase UCRT v6 mutant with improved thermal stability, the reverse transcriptase UCRT v6 mutant comprises four single-point mutants, six double mutants and four three-point mutants, and compared with wild type reverse transcriptase UCRT v6, the half-life period of the mutant is longer at 65 DEG C; the three mutants are better in effect, and the half-life period of the three mutants is about 5 times that of the wild reverse transcriptase UCRT v6. The invention also provides a genetically engineered bacterium containing the mutant. The reverse transcriptase UCRT v6 mutant obtained by the construction method provided by the invention has better thermal stability, shows higher thermal stability when being used for reverse transcription of RNA (Ribonucleic Acid) into cDNA (Complementary Deoxyribonucleic Acid) at higher temperature, and has higher application potential.