141 results about "Molecular probe" patented technology

The application relates to a DNAzyme-based gene chip biosensor and application thereof, and belongs to the technical field of electrochemical sensors. In order to solve the technical problems of low specificity and accuracy, long processing time and high cost in the single nucleotide polymorphism detection method in the prior art, a DNAzyme-based gene chip biosensor is provided, which comprises a DNAzyme molecular probe SNP recognition system and a solid-liquid phase mixed catalytic network system. The application provides a proteinase-free and space-oriented biosensing strategy, utilizes a molecular switch based on a DNAzyme probe to realize accurate SNP recognition, utilizes a space steric hindrance parallel amplification system to enhance signal amplification and simultaneously inhibit background noise, so that the detection performance is remarkably improved; and the gene chip biosensor can be applied to genotyping and trait selection in crop improvement and other applications.

The invention discloses a near-infrared two-region aggregation-induced emission probe, nanoparticles, a preparation method and application, and relates to the technical field of fluorescent molecular probes. The structural formula of the near-infrared two-region aggregation-induced emission probe is shown in the specification, wherein X1, X2 and Y are respectively and independently selected from one of O, S, Se and Te; r1, R2 and R3 are respectively and independently selected from one of H, F, cyano, trifluoromethyl and saturated alkane. The probe provided by the invention has relatively high luminous efficiency, photo-thermal efficiency and active oxygen generation capability in an aggregation state, and can realize fluorescence, photo-acoustic and photo-thermal multi-mode imaging and photo-thermal / photodynamic synergistic treatment.

This invention discloses an AfBPP-based active molecular probe for thiostreptin, its preparation, and its application, belonging to the field of chemical biology. This invention introduces a bifunctional tag integrating a photocrosslinking group (bisacrididine) and a bioorthogonal reactive group (alkynyl group) into the structure of thiostreptin. While retaining the original biological activity of the parent compound, it endows the probe with highly efficient probe function, solving the technical problem of target loss during washing and purification of traditional non-covalent probes. After target labeling, the probe can specifically connect to reporter groups such as fluorescein or biotin through click-chemical reactions, achieving efficient enrichment of drug targets. Combined with mass spectrometry analysis, it enables global identification of potential targets in cells or complex biological samples at the omics level, such as chemical proteomics, providing a powerful molecular tool for in-depth revelation of the potential targets and pharmacological mechanisms of thiostreptin.

The present invention provides a compound with which a molecular probe capable of distinguishing NSCs from CSCs can be produced, and a method for detecting cancer stem cells using the compound. The present invention relates to a compound having a formyl group, a substrate moiety, a quenching moiety, and a luminescent moiety that is non-luminous due to the quenching moiety, wherein the luminescent moiety may be rendered luminous by detaching the substrate moiety by a substrate-degrading enzyme and also converting the formyl group to a carboxy group by an aldehyde dehydrogenase to allow the quenching moiety to dissociate.

The invention relates to the technical field of tumor molecular imaging, nuclear medicine diagnosis and targeted therapy, and discloses a Trop2-targeted polypeptide, a Trop2-targeted molecular probe and application of the Trop2-targeted polypeptide and the Trop2-targeted molecular probe. The polypeptide can be coupled with different imaging elements to construct molecular probes or radiopharmaceuticals for tumor targeted imaging, so that noninvasive visual detection on the Trop2 expression level in tumor tissues is realized. The probe can specifically recognize Trop2 positive solid tumors, and a new technical means is provided for noninvasive diagnosis and dynamic monitoring of various Trop2 high-expression tumors such as pancreatic cancer, lung cancer, breast cancer and thyroid cancer. The probe disclosed by the invention has the advantages of simple preparation process, low cost, high specificity and stability, short imaging period, low radiation dosage, easiness in clinical transformation and the like, and has a wide application prospect in precise diagnosis and individualized treatment of tumors.

The application discloses a specific nucleic acid aptamer for a heterogeneous core ribonucleoprotein hnRNPA2B1 and application, and belongs to the technical field of nucleic acid aptamer. The hnRNPA2B1 aptamer is a small single-stranded DNA screened from a designed oligonucleotide library through an in-vitro exponential enrichment ligand system evolution (SELEX), is composed of 80 nucleotides, and has high affinity to the target. The hnRNPA2B1 aptamer can be applied in a detection method or a detection product of the hnRNPA2B1 protein, and a targeted regulation drug or a biological preparation, and can be specifically made into a molecular probe, used as a detection reagent, used as a targeted preparation and the like for diagnosis and treatment of diseases related to the hnRNPA2B1 protein. The hnRNPA2B1 aptamer has strong affinity and specificity, has the advantages of low production cost, stability, convenient synthesis and easy modification compared with an antibody, and is a potential substitute for the antibody in disease diagnosis and treatment.

The invention provides a molecular probe targeting EDB-FN as well as a preparation method and application of the molecular probe. The structural formula of the molecular probe targeting EDB-FN is shown in the specification, according to the molecular probe targeting EDB-FN, the probe is based on a cyclic peptide probe with Lys-Glu side chain lactam bridge conformation fixed, the connection mode of a DOTA chelating agent is optimized, and the clinical transformation bottlenecks that an existing probe is poor in stability, low in affinity, insufficient in targeting specificity, poor in diagnosis and treatment integration compatibility and the like are solved. The probe can be used for early diagnosis and molecular imaging research of tumors, and provides a reliable basis for tumor malignancy degree evaluation and treatment effect monitoring.

This application discloses a linker and its preparation method. The linker includes pharmacophore P, pharmacophore Q, and deoxynucleotides and / or deoxynucleotide derivatives connecting pharmacophore P and pharmacophore Q. Pharmacophore P is obtained by removing the protecting group from group B, and pharmacophore Q is obtained by removing the protecting group from group X. Group B is obtained by chemically modifying the benzylquinolone carboxylic acid pharmacophore, and group X is obtained by chemically modifying the zenomeprazole pharmacophore. The linker is prepared by linking group B and group X with deoxynucleotides and / or deoxynucleotide derivatives to form the linker, including solid-phase synthesis technology. The method of this application can automatically synthesize molecular probes or bivalent drugs containing two pharmacophores, thereby replacing the traditional stepwise linking method, enabling efficient construction of screening libraries, and allowing control of the spatial distance and spatial orientation of the pharmacophores by adjusting the number and base arrangement of deoxynucleotides.

This invention discloses a naphthimide-based fluorescent probe for detecting HClO and its preparation method. The fluorescent molecular probe uses naphthimide as the fluorophore and C=N as the specific reaction site for HClO. Due to the presence of the naphthimide fluorophore, the probe molecule exhibits two-photon properties, enabling deep tissue penetration, reducing background noise, and facilitating fluorescence imaging of biological systems. Furthermore, the introduced morpholine group allows for lysosomal targeting, enabling the monitoring of hypochlorous acid concentration levels in lysosomes, making it a valuable tool for early disease diagnosis. In addition, this type of probe molecule is simple to synthesize, operates under mild reaction conditions, exhibits stable optical properties, and has a high synthesis yield. It can detect a wide range of HClO, making it practically valuable in fields such as biochemistry and environmental science.

The invention belongs to the technical field of early diagnosis of diseases, and discloses a polypeptide targeting macrophage PLIN2, a PET molecular probe and a preparation method and application thereof.Firstly, the polypeptide P672-1 targeting the PLIN2 is synthesized, and 64Cu-NOTA-P672-1 is prepared on the basis of the polypeptide P672-1. The molecular probe has the good chemical yield and stability, has the good targeting performance on the PLIN2, and can be used for preparing 64Cu-NOTA-P672-1. And the biological safety performance is good. Compared with the conventional 18F-FDG, the 64Cu-NOTA-P672-1 prepared by the preparation method disclosed by the invention has a good early diagnosis effect. The preparation method of the 64Cu-NOTA-P672-1 is simple and easy to implement, and the 64Cu-NOTA-P672-1 can be used for diagnosing early abdominal metastasis of ovarian cancer with high expression of PLIN2 The nuclide 64Cu-NOTA-P672-1 disclosed by the invention has important clinical significance on early diagnosis of ovarian cancer metastasis.

This invention relates to nucleic acid aptamers for lipoprotein-associated phospholipase A2 (Lp-PLA2), wherein the nucleic acid aptamer is at least one of B76-2, B76-4, and B76-5, with sequences shown in SEQ ID NO.1, SEQ ID NO.2, and SEQ ID NO.3, respectively. The Lp-PLA2 aptamers provided by this invention fill a gap in the field of Lp-PLA2 aptamers and exhibit high affinity and specificity. Three aptamers with good affinity and specificity for Lp-PLA2 have been screened and verified. These aptamers can be formulated into molecular probes or used as detection reagents in methods or kits for detecting Lp-PLA2, improving accuracy. They can also be used to develop novel Lp-PLA2 biosensing methods for molecular identification, showing great application potential in the early warning of cardiovascular disease events.

The invention relates to the technical field of biomedical detection and functional organic fluorescent materials, in particular to synthesis and application of a single-molecule probe combining BODIPY (boron dipyrromethene) and a semi-cyano dye. Comprising the following steps: preparing an intermediate 1: reacting 4-hydroxybenzaldehyde with 2, 4-dimethyl pyrrole to generate BDP-OMe, and then reacting with pyridinium chlorochromate to obtain the intermediate 1; preparation of an intermediate 2: carrying out reflux reaction on 2, 3, 3-trimethyl-3H-indole and 2-bromoethanol in ethanol to obtain the intermediate 2; and synthesizing a final product: heating the intermediate 1 and the intermediate 2 in toluene to react, and performing post-treatment and column chromatography purification to obtain the probe CyB-C. The bimodal detection capability enables the probe to simultaneously distinguish the microenvironment difference between normal cells and cancer cells and monitor diseases related to viscosity abnormity.

The application discloses a polypeptide PET molecular probe for targeting breast cancer and a preparation method and application thereof. The polypeptide PET molecular probe for targeting breast cancer is obtained by labeling an AR polypeptide modified by a bifunctional chelating agent with a positron emitting radionuclide, and has the advantages of simple synthesis, small molecular weight, high specificity, no immunogenicity and good biological safety. In addition, the polypeptide PET molecular probe has high radiochemical performance and in-vivo and in-vitro stability, can better realize breast cancer targeted PET imaging compared with current commonly used imaging agents, can be used for preparing an imaging agent for breast cancer diagnosis, and is helpful for clinical diagnosis of breast cancer.

The application relates to the field of biological medicine, and particularly discloses application of a polypeptide in preparation of a specific targeting molecular probe for tumors with DLL3 overexpression. The application finds that a molecular probe prepared from a polypeptide shown in SEQ ID No. 4 can effectively target tumor tissues with DLL3 overexpression, thereby providing application of a polypeptide or a dimer or a multimer thereof in preparation of a specific targeting molecular probe for tumors with DLL3 overexpression, wherein the amino acid sequence of the polypeptide is shown in SEQ ID No. 4. The molecular probe of the application has high imaging precision, good affinity with DLL3, can realize early diagnosis and imaging of tumor tissues, and can also be used for preoperative and intraoperative image navigation, thereby improving the operation accuracy.

This invention discloses a radioactive molecular probe for targeting CSPG4 / NG2, its preparation method, and its application. The radioactive molecular probe comprises the structure shown in Formula I or Formula II. The technical solution of this invention uses a radioactive molecular probe to specifically recognize CSPG4 / NG2 in vivo through the target polypeptide portion, thereby achieving specific nuclear medicine positron emission tomography (PET) or single-photon emission computed tomography (SPECT) imaging diagnosis of CSPG4 / NG2 positive tumors or CSPG4 / NG2 positive lesions, such as autoimmune diseases and cardiovascular and cerebrovascular diseases, thereby realizing early diagnosis and efficacy evaluation of malignant tumors.

This invention discloses a dihydroisoxazole molecule, a method for synthesizing the compound, and its pharmaceutical applications. The dihydroisoxazole molecule of this invention contains a pyridine group, which is significant for molecule modification in the later stages of pyridine drug synthesis and for accelerating the screening of clinical drug candidates. The dihydroisoxazole molecule contains coordinating nitrogen and oxygen atoms; as a ligand, the synthesized nitrogen-containing bicyclic compound can be used to design metal catalysts; as a molecular probe, the dihydroisoxazole molecule can be used for drug candidate discovery; and as a scaffold, the dihydroisoxazole molecule can be used in materials science.

The application discloses an expression vector of a biosynthetic metabolic pathway gene of 2-azacyclohexanone and a synthesis method of 2-azacyclohexanone. The expression vector comprises a first promoter and, sequentially inserted downstream of the first promoter, davA-davB genes or davB-davA genes, a Pb promoter or a mutant thereof, an act gene or an orf26 gene or a caiC gene, a ChnR gene or a mutant thereof. The expression vector is further transferred into glutamic acid corynebacterium with high lysine yield, and is subjected to fermentation culture to efficiently synthesize 2-azacyclohexanone. The application synthesizes 2-azacyclohexanone in glutamic acid corynebacterium for the first time, and utilizes an optimized 2-azacyclohexanone molecular probe system to start the expression of key genes in the 2-azacyclohexanone biosynthetic metabolic pathway for the first time, so that high yield of 2-azacyclohexanone is realized. The synthesis method reduces the production cost required by using lysine as a synthesis raw material, eliminates a rate-limiting step, and greatly improves the biosynthesis yield of 2-azacyclohexanone.

The invention relates to the technical field of biosystem molecular probes, in particular to a lipid droplet protein in-situ labeling probe as well as a preparation method and application thereof. The structure of the probe is shown in the specification. The synthesis method of the probe is simple and easy to implement, the probe can be specifically positioned at the lipophilic end of the phospholipid monomolecular layer of the lipid droplet and the lipid center area, and the probe can be used for in-situ and real-time marking of lipid droplet protein in living cells and animal bodies. According to the technology, the in-situ analysis technology of the lipid droplet protein from a cell level to an animal level is realized for the first time, a powerful tool is provided for systematically analyzing the protein composition and the dynamic evolution rule of the lipid droplet, and the technology has a wide application prospect.

The invention discloses an RPA-CRISPR / Cas12a system for detecting a cowfish based on environmental DNA (Deoxyribonucleic Acid) and a specific primer, and belongs to the technical field of biological detection. Aiming at the sequence of a COI fragment of a mitochondrial genome of the Jiangsu, an RPA primer with the species specificity of the Jiangsu is designed so as to amplify a target fragment; designing a high-activity and specific crRNA sequence by searching a Cas12a protein recognition site in a corresponding amplification sequence so as to guide the Cas12a protein to recognize a target fragment and activate the trans-cleavage activity of the Cas12a protein; by designing a molecular probe with stable performance, the probe is stably and efficiently cut by a Cas12a-crRNA active compound, and a detection result can be observed in real time through a luminoscope. The detection method is easy to operate, high in sensitivity and good in specificity, detection can be completed only through constant-temperature heating equipment, and a new technology and method are provided for rapid detection and protection of the cowfish.

The invention discloses a double-target small-molecule probe, a radionuclide-labeled double-target small-molecule probe as well as a preparation method and application of the radionuclide-labeled double-target small-molecule probe. The medicine < 68 > Ga-DOTA-PRE / RGD is obtained by labeling < 68 > Ga radionuclide, and is applied to PET / MR (positron emission tomography / magnetic resonance imaging) imaging of pancreatic cancer tumors. A radioactive product with high purity and high stability (greater than 93%) is obtained through a simple marking method; the obtained double-target radioactive small molecular probe 68Ga-DOTA-PRE / RGD has a huge potential for the accurate diagnosis of pancreatic cancer.

This invention discloses a tandem-responsive self-assembled molecular probe and its applications. The HPO-CYFF-TPP of this invention is converted to HO-CYFF-TPP under the dephosphorylation of overexpressed ALP, restoring the intramolecular charge transfer effect. It then self-assembles into nanoparticles in situ through π-π stacking, exhibiting fluorescence signal quenching and photoacoustic signal enhancement at low viscosity. Upon laser irradiation, the NPs generate a large amount of singlet oxygen in the mitochondria, thereby achieving efficient photodynamic therapy (PDT) and leading to increased viscosity. At high viscosity, due to the suppression of the distorted intramolecular charge transfer effect of HO-CYFF-TPP, the NPs activate the FL signal with a high signal-to-noise ratio and further enhance the PA signal. Therefore, the HPO-CYFF-TPP of this invention can achieve efficient PDT and enable real-time therapeutic effect assessment through FL / PA signals.

The present application relates to a kind of water-soluble carbohydrazide derivative and its preparation method, the method for detecting ion or amino acid, belong to organic small molecule probe technical field.The present application introduces different pyridyl on the hydrazine group of carbohydrazide, obtains a kind of pyridyl substituted carbohydrazide derivative.Compared with existing carbohydrazide compound, the recognition function of pyridyl substituted carbohydrazide derivative provided by the present application has been expanded and regulated, can be used for Cu 2+ , Hg 2+ , Ca 2+ , Cd 2+ , Zn 2+ , AcO ‑ , HSO4 ‑ And glutamic acid (Glu) / L-Asp, lysine (Lys) / arginine (Arg) recognition detection.The water-soluble carbohydrazide derivative provided by the present application as molecular probe, its structure is stable, synthesis method is simple, has the ability of responding to multiple substrates, and water-soluble, has wide application prospect.

The application provides a method for monitoring lipid oxidation tendency, and belongs to the technical field of fluorescent probe detection. The method utilizes the fluorescence characteristics of a BODIPY molecular probe, and through selecting a molecular probe with a specific structure, directional reaction occurs between the BODIPY molecular probe and oxygen free radicals generated by lipid oxidation at a specific pH value. The conjugated system of the BODIPY molecular probe is destroyed, the half-chlorin structure is broken, and the fluorescence color changes from red to blue-green. According to the change of the fluorescence color before and after the reaction, the visualization monitoring of the lipid oxidation tendency and spatial distribution is realized through fluorescence imaging.

The invention relates to the technical field of biosensing, and discloses a pH / viscosity double-lock activated afterglow luminescent small molecule probe as well as a preparation method and application thereof. The structural formula of the afterglow luminescent small-molecule probe is shown in the specification. A rhodamine light-emitting unit and an afterglow light-emitting matrix unit SO are covalently connected through a chemical bond. The luminescent mechanism is as follows: under illumination, molecules generate singlet oxygen to oxidize and activate adjacent SO units, so that active oxygen mediated afterglow luminescence is generated. The afterglow luminescent small-molecule probe has a double-lock activation characteristic, that is, a strong afterglow signal can be generated only when a subacid environment and a high-viscosity condition are met at the same time. And the signal is weak under a single condition or a normal physiological environment. The probe provided by the invention has excellent environmental specificity and high signal-to-noise ratio imaging capability, and provides a powerful tool for realizing accurate imaging in a complex biological system.

The application discloses an organic light-emitting material with chiral and narrow-band emission performance, a preparation method and application thereof, and the core is that multiple resonance structures can effectively inhibit the vibration coupling between S1-S0 transition and excited state structure relaxation, form significant narrow-band emission, and simultaneously utilize the axial chirality characteristics of BINOL, utilize a chiral disturbance strategy to form circularly polarized luminescence. The application further provides a preparation method of the material, wherein compound B is obtained by reacting compound C with compound D, and the organic light-emitting material is obtained by reacting compound A with compound B. The material has the characteristics of thermal activated delayed fluorescence induced by multiple resonance effect, and simultaneously has the characteristics of circularly polarized luminescence, and can be applied to the fields of 3D display, molecular probe, spin information, optical data storage and processing and optoelectronic devices, and the like. The synthesis process is simple, raw materials are widely sourced and inexpensive, and the material is suitable for wide application.

This invention relates to the field of molecular probe technology for biological systems, and particularly to an in-situ labeling probe for lipid droplet proteins, its preparation method, and its applications. The probe of this invention has the following structure: [structure omitted]. The synthesis method of this probe is simple and easy to implement, and it can specifically locate the lipophilic ends and lipid center regions of the phospholipid monolayer of lipid droplets. It can be used for in-situ, real-time labeling of lipid droplet proteins in living cells and animals. This technology represents the first significant leap from cellular to animal-level in-situ analysis of lipid droplet proteins, providing a powerful tool for systematically elucidating the protein composition and dynamic evolution of lipid droplets, and has broad application prospects.

This invention belongs to the field of fungal detection technology, specifically relating to a micro-platform for rapid electrolysis and highly sensitive electrochemical nucleic acid detection of Cryptococcus neoformans and its application. The micro-platform includes an integrated microfluidic chip, an external power supply, a sample introduction system, and / or an imaging system. The integrated microfluidic chip includes an electrolysis region, an electrochemical detection region, and an electrode array region. The electrolysis region includes multiple vertically arranged counter electrodes arranged in pairs, and multiple horizontally arranged hollow channels in a serpentine pattern, bonded to the electrode pairs above them. The connection point between every two electroporated channels is the electroporation lysis point. The electrochemical detection region consists of a working electrode, a reference electrode, and a counter electrode. The working electrode is modified with rGO / AuNPs nanomaterials and molecular probes.

The invention discloses an ROS (reactive oxygen species) efficient synchronous recognition system combined with multiple molecular probes and application. The system comprises a table type vertical rotating device, a first reaction device and a detection device, the first reaction device is detachably fixed on the table type vertical rotating device in a dark place and comprises a reaction medium and a pollutant-probe mixed solution; the reaction medium is periodically kept in a wetting-exposure alternating state in the vertical rotation process; the first reaction device comprises three parallel and independent pollutant-probe mixed solutions which are respectively used for identifying. OH, O2. <-> and < 1 > O2; and the detection device is used for quantitatively determining the residual concentration of a stable product or a probe reagent after the reaction of each probe and the free radical. The method has the advantages of being high in scene adaptability, easy and convenient to operate, low in cost, high in sensitivity, high in matrix interference resistance and the like, and can be widely applied to exploration and research of natural attenuation or enhanced attenuation mechanisms and the like of pollutants in saturated or non-(variable) saturated scenes.

The present application relates to the field of biological medicine, and provide a kind of targeting PSMA dimer compound and its derivative and application, the structure general formula of the dimer compound is as shown in formula (I).The dimer compound and its derivative of the present application have very high affinity and targeting to PSMA, after being labeled with suitable radionuclide, can be used as nuclear medicine molecular probe, to realize early diagnosis, staging and treatment of prostate cancer, 68 Ga]diPSMA-1-DOTA showed good tumor uptake in PET imaging of tumor mice.Therefore, this kind of radioactive complex has good clinical application prospect in tumor imaging targeting PSMA (especially PET imaging), and lays an important foundation for the radiotherapy application of [ 177 Lu]diPSMA-1-DOTA and [ 225 Ac]diPSMA-1-DOTA.

The invention relates to the technical field of biological medicine, in particular to a cell membrane labeled molecular probe, a preparation method and application of the cell membrane labeled molecular probe in the imaging direction of drug-resistant bacteria, and the preparation method of the cell membrane labeled molecular probe comprises the following steps: step 1, mixing N, N '-carbonyldiimidazole and nonanoic acid for reaction to obtain active ester, tert-butyl (2-aminoethyl) carbamate is added for a reaction, and tert-butyl (2-nonanoylamido ethyl) carbamate is obtained; step 2, the tert-butyl (2-nonanoylamido ethyl) carbamate and trifluoroacetic acid are subjected to a reaction, and N-(2-aminoethyl) nonanoylamides are obtained; 3, at the room temperature, fluorescein isothiocyanate ester, N-(2-aminoethyl) nonanamide and triethylamine are subjected to a reaction, and the molecular probe is obtained. The molecular probe prepared by the invention has a stable chemical structure, is not easy to degrade or decompose in a physiological environment, can maintain long-time fluorescence performance, and is suitable for long-time cell membrane marking and imaging experiments.