141 results about "Mixed culture" patented technology

The use of an SNP site combination of Litopenaeus vannamei in trait evaluation of mixed-family culture, a probe and a kit. The SNP site combination of Litopenaeus vannamei comprises 1125 SNP sites, wherein the physical positions are determined on the basis of alignment with a reference genome sequence of Litopenaeus vannamei. Also disclosed are a molecular probe for capturing the SNP site combination and a kit comprising the molecular probe. By means of using the SNP site combination for paternity identification, the present invention can achieve the mixed culture of individuals of different families in the early stage, so that the common environmental effect caused by separate family culture in the early stage is effectively reduced, and the accuracy of a trait evaluation result of mixed culture is improved; moreover, the genetic relationship between individuals can be accurately calculated. Compared with physical marks, the present invention avoids the phenomena of falling and dislocation of marks on individuals and human identification errors, thus obtaining accurate pedigrees.

A preparation method of a rice straw biochar loaded with Bacillus cereus, comprising: (1) washing, drying, grinding and sieving rice straws; (2) performing anaerobic pyrolysis treatment on the rice straws treated in the step (1) at 300-700° C. to obtain a biochar; (3) treating the biochar obtained in the step (2) with hydrochloric acid, then washing until a washing solution is neutral, drying, grinding, sieving and sterilizing to obtain a sterilized biochar; and (4) performing mixed culture on the sterilized biochar and a solution of Bacillus cereus subjected to activation culture, and then centrifuging after the end of culture to remove supernatant, so as to obtain a rice straw biochar loaded with Bacillus cereus having a carbon immobilizing capability.

The invention relates to the technical field of microorganisms, in particular to compound probiotics capable of degrading indole and preventing or relieving nephropathy and application of the compound probiotics. The compound probiotics comprise bifidobacterium animalis subsp. Lactis YYS-J9 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) NO.28185 and bifidobacterium longum subsp. Longum YYS-BSM8 with the preservation number of CGMCC NO.28611. The invention further discloses a preparation method of the compound probiotics. Compared with the use of a single YYS-J9 strain or YYS-BSM8 strain, the two strains have synergistic effects after being compounded, the viable count is remarkably increased after mixed culture, the inhibition effect on pathogenic enterobacteria and the adhesion to intestinal cells are remarkably enhanced, the degradation capacity on indole and oxalic acid is higher, the proliferation and phagocytic activity of macrophages is remarkably improved, and the application range of the YYS-J9 strain and the YYS-BSM8 strain is widened. And when being applied to food, health-care products or medicines, the exopolysaccharide can be used for effectively preventing or improving nephropathy and kidney stone, regulating intestinal health and improving intestinal immunity.

The invention discloses a compound microbial agent for preventing and treating fusarium wilt of cotton and application of the compound microbial agent, and belongs to the technical field of biological prevention and treatment of plant diseases. The prepared compound microbial agent is obtained by mixed culture of clostridium butyricum JBH-BD1 fermentation centrifugate, enterococcus faecium JBHBIO-BSC1, glucose and bacillus subtilis JBH-BK8, the preparation method is simple, and industrialized production, popularization and application are facilitated. The compound microbial agent has an obvious inhibition effect on cotton fusarium wilt caused by fusarium oxysporum, and the bacteriostasis rate reaches 66.7%. The harm of the cotton fusarium wilt to cotton is effectively reduced by utilizing a biotechnology, meanwhile, compared with a chemical agent, the problems of environmental pollution and the like are effectively avoided, and the application prospect is very wide.

The invention discloses a bioreactor for stretching culture of artificial tendons, and relates to the technical field of bioreactors, the bioreactor comprises an incubator, a supporting plate and a rectangular sliding groove formed in the supporting plate, one side of the incubator is fixedly provided with a rotary swing mechanism, one end of the rotary swing mechanism is connected in series with a plurality of groups of stretching culture modules, and the stretching culture modules are arranged in the rectangular sliding groove. When a strip-shaped toothed plate slides in a reciprocating mode, a push-pull rod on a culture solution balancing mechanism and a piston assembly can be driven to move in a reciprocating mode in a cylinder through a connecting rod piece, and when the piston assembly moves in the cylinder in a reciprocating mode, culture solutions at all positions in a rectangular culture dish can be sucked into the cylinder to be mixed through a liquid suction pipe fitting; then the mixed culture solution in the cylinder can be circularly discharged into each part in the rectangular culture dish through the liquid outlet pipe fitting, so that the hydrodynamic environment in the reactor can be improved, and the influence on the stretching culture effect of the artificial tendon due to non-uniform deposition and distribution of the culture solution can be effectively avoided.

The invention relates to a method and system for in-situ treatment of fluorine-containing mine water in cooperation with a coal mine underground reservoir. The method comprises the following steps: S1, screening coal gangue of which the coal content is 5-20% and the particle size is less than or equal to 5mm; s2, fluorine-containing mine water or sediment collected near the site to be treated is used as a sample, the sample is subjected to screening, enrichment and domestication of mixed anaerobic flora, and efficient defluorination mixed bacteria are obtained; s3, the bacterial liquid of the efficient defluorination mixed bacteria and the coal gangue are subjected to mixed culture, and microbial biofilm culturing coal gangue is obtained; and S4, adding the microbial biofilm formation coal gangue into a material adding mechanism, and adding the microbial biofilm formation coal gangue into mine water of a coal mine underground reservoir through the material adding mechanism for defluorination treatment. According to the invention, in-situ defluorination can be carried out on the mine water of the coal mine underground reservoir, and the purification treatment effect of the coal mine underground reservoir on the mine water is greatly improved.

The invention discloses a multi-species mutualism-based mussel and fish polyculture ecological regulation and control method, and relates to the technical field of ecological culture, and the method comprises the following steps: S1, facility preparation; s2, putting species; s3, breeding regulation and control; s4, feeding control; and S5, performing final-period management. Through strict screening of the culture pond, thorough disinfection and scientific cultivation of water quality, an excellent starting end is created for culture, the disease risk is reduced, beneficial microbial communities enhance the self-cleaning capacity of a water body, the feeding quantity and proportion are accurately calculated, the ecological capacity is fully utilized, the fingerlings are reasonably matched, stable operation of an ecological system is guaranteed, and the ecological system is more intelligent and reliable through real-time monitoring and intelligent prediction. The feeding amount and the water changing frequency are adjusted in time, the water quality is effectively maintained, the breeding loss caused by water quality deterioration is reduced, grass carp feeding is adjusted according to the aquatic plant coverage rate, reasonable growth of aquatic plants is maintained, ecological balance is promoted, the win-win situation of ecological benefits and economic benefits is achieved, and an effective mode is provided for sustainable aquaculture.

The invention discloses a probiotic double-layer coating film applied to aquatic products and a preparation method of the probiotic double-layer coating film, and relates to the field of aquatic product processing and preservation. The method comprises the following specific steps: preparing a film coating solution A and a film coating solution B, soaking an aquatic product in the film coating solution A containing lactobacillus rhamnosus and lactobacillus plantarum for primary film coating, drying, and soaking the aquatic product in the film coating solution B containing zein for secondary film coating. Wherein the coating solution A is prepared by mixing a lactobacillus rhamnosus culture solution and a lactobacillus plantarum culture solution, dissolving sodium alginate in a pre-fermented mixed culture solution, uniformly stirring, adding sterile glycerol, and fully and uniformly mixing; the film coating solution B is prepared by dissolving zein in ethanol, stirring at 50-60 DEG C for 1-2 hours, then adding sterile glycerol, fully and uniformly mixing, and cooling to room temperature. The aquatic products are treated by adopting a probiotic double-layer coating film, so that the aquatic products have a fresh-keeping effect and a probiotic effect, water loss of the aquatic products can be delayed, flavor substances in the aquatic products can be prevented from volatilizing, microorganism growth and protein oxidation in the storage period can be inhibited, and odor and taste deterioration in the storage process of the aquatic products can be delayed; the storage quality of aquatic products is effectively improved and the shelf life is prolonged.

The invention discloses a siraitia grosvenorii pomace extract as well as an extraction method and application thereof. Relates to the technical field of biological fermentation and natural product extraction. Comprising the following steps: strain activation and mixed culture; biological fermentation pretreatment; decoloring and eluting; and enriching and drying. Through enzyme system complementation of compound microorganisms, cell wall structures of the siraitia grosvenorii residues are degraded in stages, saponin, polyphenol, flavone and polysaccharide are synchronously released, and the biological activity is remarkably improved. The layer-by-layer decomposition of the siraitia grosvenorii residue fiber structure is realized by adopting sectional fermentation, and the dissolution of functional components is promoted; ultrasonic synergistic auxiliary extraction is combined, so that efficient wall breaking and component dissolution are realized; the steps of ultrasonic extraction, macroporous resin decoloration and the like are designed in series, so that the solvent dosage is reduced by 50%, and the energy consumption is reduced by 35%; the gradient elution realizes simultaneous collection of functional components such as saponin, flavone, polyphenol and the like. The extract can be directly used for functional food, medicines or cosmetics, and the fermentation liquor can be processed into the momordica grosvenori flavored lactic acid bacteria drink.

The invention belongs to the technical field of biological manufacturing, and relates to an Euglena gracilis algal strain ZC-1007 with high yield of beta-1, 3-glucan and a preparation method of the Euglena gracilis algal strain ZC-1007. The preservation number of the algal strain ZC-1007 is GDMCC (China General Microbiological Culture Collection Center) No.66479. The algal strain is high in biomass density, high in growth speed and high in beta-1, 3-glucan yield under the light polyculture condition. The preparation method of the euglena strain comprises the steps of optimizing culture conditions and efficiently screening, and obtaining the euglena strain ZC-1007 with high yield of beta-1, 3-glucan through a specific culture medium and environment control. The strain has a good application prospect in the aspect of efficiently preparing a functional component beta-1, 3-glucan.

The invention relates to a method for producing higher alcohols. The invention also relates to a reaction mixture and a method for producing at least one higher alcohol, said method comprising a reaction mixture comprising a mixed culture of a first and a second microorganism in an aqueous medium comprising carbon monoxide gas, wherein the first microorganism is an acetogenic microorganism capable of converting a carbon source to acetic acid and / or ethanol; and-the second microorganism is selected from the group consisting of Clostridium kluyveri capable of converting acetic acid and / or ethanol to form an acid, and C. carboxidivorans; wherein the first microorganism is further capable of converting the acid to the corresponding higher alcohol, and the higher alcohol comprises at least 6 carbon atoms.

The invention belongs to the technical field of aquaculture tail water treatment, and discloses a marine aquaculture tail water denitrification device based on elemental sulfur conversion and a method thereof. According to the device, agricultural wastes or high-molecular polymers are added into elemental sulfur to form an anaerobic sulfenyl polyculture denitrification system, and the anaerobic sulfenyl polyculture denitrification system is used for bearing filler and water pressure through a bearing layer arranged from the bottom; a sulfur autotrophy and heterotrophy synergistic polyculture denitrification system is formed through a functional layer arranged in the middle, and the pH value in the polyculture denitrification system is maintained; and intercepting the fallen biological membrane and suspended matters through a filter layer arranged at the top. According to the invention, heterotrophic denitrification and sulfur autotrophic denitrification technologies are combined to form a solid-phase sulfur-based polyculture denitrification system, elemental sulfur is converted into polysulfide / sulfide, conversion of sulfur is promoted, biocompatibility is improved, denitrification efficiency is improved, starting time of equipment is shortened, and the problem of carbon source dependence is effectively solved.

The application belongs to the technical field of microbial fermentation, and particularly relates to a kind of cytolysis product of mixed bacteria fermentation and a preparation method. The cytolysis product is prepared by mixed bacteria fermentation of two kinds of aerobic probiotics, i.e. Lactobacillus plantarum and Aspergillus oryzae are used as fermentation strains to carry out mixed bacteria fermentation treatment on rice milk, and membrane filtration and column chromatography system are used for filtration and enrichment, so as to obtain a cytolysis fermentation product filtrate rich in kojic acid, the content of kojic acid is greater than 200 g / L, which is significantly improved compared with conventional process. The obtained cytolysis product has no bad smell and clear color, so that the beneficial function of kojic acid can be played only with a low addition amount in cosmetics, and the addition of odor masking and color correcting auxiliaries is reduced, and the cytolysis product can be used for preparing cosmetics with skin repair, whitening and other functions.

The invention relates to a method for producing a water environment protective agent by fermenting hermetia illucens larva slurry, and belongs to the technical field of microbial fermentation. According to the present invention, the hermetia illucens larvae are adopted as the main raw material, the main raw material is ground into the slurry through the colloid mill, the small amount of the carbon source is supplemented, the Phaffia rhodozyma CGMCC NO.29217, the Bacillus coagulans CGMCC NO.5233 and the Clostridium butyricum CGMCC NO.4729 are subjected to the mixed bacteria synergistic fermentation, and the produced product can be widely used in the aquaculture and other application scenarios. Fermented hermetia illucens larva serous fluid is rich in astaxanthin, a fermented product has obvious acid fragrance and no peculiar smell, the total number of probiotics is larger than or equal to 5.0 * 10 < 8 > cfu / g, the pH value is smaller than 4.50, and the content of small molecule active polypeptide (acid soluble protein) of the fermented product is increased by 2.5-3 times compared with that before fermentation. Bacillus subtilis CGMCC (B) 63501 is used as indicator bacteria to detect that the content of bacteriostatic active substances after fermentation of hermetia illucens larva serous fluid is larger than 1.0 g / kg, pathogenic bacteria such as salmonella, vibrio parahaemolyticus and vibrio cholerae are not detected, and normal-temperature storage can be achieved. The preparation method is low in production cost, easy to operate, safe and free of residues, and the economic benefits of aquaculture can be improved.

The application provides a solid substrate for soilless culture prepared by fermentation of coffee residue and mixed bacteria, wherein the mixed bacteria include yeast, bacillus subtilis and trichoderma viride; the weight ratio of the yeast, the bacillus subtilis and the trichoderma viride is 2:1:3. The solid substrate for soilless culture is rich in trace elements required by plant growth, has low bulk density, high water holding porosity, and is controllable. The solid substrate for soilless culture can effectively improve the plant growth environment, has disease resistance and insect resistance, and improves the survival rate of plants.

The invention discloses a fish and shellfish polyculture experiment system and a verification method, and the system comprises at least two groups of experiment units which operate independently, an intelligent water distribution unit, a water quality on-line monitoring unit, a waste automatic collection unit and a central control unit. Wherein the experiment module comprises a fish culture area, a shellfish culture area, an adjustable mainstream circulation channel and a bypass circulation loop. The verification method comprises the steps of system initialization and experiment grouping, biological putting and feeding calibration, breeding operation under a controlled condition, multi-processing-group synchronous monitoring and sampling and data analysis and efficiency comprehensive evaluation. According to the method, the experimental platform which can accurately simulate the material circulation process of the fish-shellfish polyculture system, realize synchronous comparison of multiple treatment groups and quantitatively evaluate the nutrient interstage material coupling mechanism is constructed, and a scientific basis is provided for an ecological culture mode with low emission and high resource utilization rate.

The invention discloses a method for culturing fungal-feeding nematodes by using two fungi in a mixed manner and reducing pollution of a culture medium. The method comprises the following steps: A, separating and extracting euphorbia fungal-feeding nematodes; b, identifying the euphorbia fungal nematodes; c, preparing a strain mixture which comprises botrytis cinerea and trichoderma harzianum in a mass ratio of 1: 1; d, inoculating the strain mixture into a PDA liquid culture medium to prepare a nematode PDA culture medium; e, centrifuging the nematodes obtained in the step B; f, disinfecting the nematodes, namely disinfecting the surfaces of the nematodes by using a mixed solution of streptomycin sulfate and actinomycete ketone; g, adding sterile water into the nematodes disinfected in the step F, and centrifuging; h, repeating the step G for multiple times to obtain nematode liquid; i, inoculating the disinfected nematodes into a flat plate of a PDA solid culture medium; and J, observing the growth quantity of the nematodes in the culture medium by using an inverted microscope. According to the method, the nematodes are cultured by culturing the fungus-eating nematode strain mixture, and the method is used for culturing and propagating the nematodes.

This invention relates to the field of microbial fermentation technology, specifically to a Monascus purpureus strain with strong stress resistance and high esterase production, and its applications. The Monascus purpureus strain is *Monascus purpureus* (…). Monascus ruber ZH01, deposited on October 20, 2025, at the China Center for Type Culture Collection (CCTCC), accession number: CCTCC NO:M 20252263. This *Monascus purpureus* strain exhibits strong resistance to adverse conditions and can grow normally in an environment with pH 3 and 25% ethanol. Its fermentation products, after being dried at 50°C for 24 hours, still maintain a higher survival rate compared to the control. Furthermore, it can produce high levels of esterifying enzymes in mixed culture media, with an esterification power of 165.8 mg / g·100h. During the fermentation of baijiu and vinegar, esterification generates lipids, enhancing the flavor of the beverages. The production of esterified red yeast rice using *Monascus purpureus* ZH01 of this invention has advantages such as short production cycle, low cost, and high esterification power.

The present invention is a method for producing a fermentation broth containing colloidal particles having a particle size of not more than 50 nm and short-chain fatty acids, with an acidity of pH 3 to 4. The method is a method comprising the steps of: a step of preparing a fermentation device comprising a plurality of sets of fermentation vessels subjected to temperature management in each stage of a multi-stage fermentation process; a step of using soft water as a starter culture, a spore-forming Clostridium genus-containing primary culture solution containing 7 kinds of fermentation bacteria (International Depositary Accession Nos. NITE BP-02945 to NITE BP-02951) managed at a low temperature, and 3 kinds of fermentation media derived from natural raw materials generated by separately fermenting a first culture medium of dried soybeans, a second culture medium of a mixed culture medium of dried plants containing Chinese jujube, Chinese wolfberry fruit, and turmeric, and a third culture medium of a honey raw material; and a step of producing a fermentation broth by a multi-stage fermentation process.

The invention belongs to the technical field of environmental engineering and pollution treatment, and particularly relates to an ecological intensive mariculture tail water intelligent treatment system and method.The ecological intensive mariculture tail water intelligent treatment system comprises a pretreatment and solid waste recycling module, a primary purification module, a biological collaborative purification module and a deep purification module; the pretreatment and solid waste recycling module comprises a high-density sedimentation tank, the primary purification module comprises a microalgae open pond, the biological collaborative purification module comprises a mixed culture area, and the deep purification module comprises an artificial wetland area. An outlet in the upper end of the microalgae open pond is connected with an inlet in the upper end of the mixed culture area through a pipeline I; according to the system, a biological treatment mode is adopted, the inherent defect of a single method in the prior art is overcome, standard discharge of mariculture tail water is achieved, green, ecological and efficient development of the breeding industry is promoted, and the win-win situation of ecological environment protection and economic benefit improvement is achieved.

The application belongs to the technical field of biological manufacturing, and relates to a kind of high-yield beta-1,3-glucan fine euglena (Euglena gracilis) strain ZC-1007 and its preparation method. Euglena gracilis ​ The preservation number of the strain ZC-1007 is GDMCC No.66479. The strain has high biomass density, fast growth rate and high yield of beta-1,3-glucan under light mixed culture conditions. The preparation method of the strain includes optimization of culture conditions and high-efficiency screening. Through specific culture medium and environmental control, the high-yield beta-1,3-glucan euglena strain ZC-1007 is obtained. The strain has good application prospect in the efficient preparation of functional component beta-1,3-glucan.

The utility model relates to the technical field of organic fertilizer mixed culture, and discloses a soil functional bacteria organic fertilizer mixed culture device which comprises a feeding bin, a feeding conveyor is arranged on one side of the feeding bin, and the bottom of the feeding bin is connected with a smashing assembly, a conveying bin, a conveying motor and an auger shaft through feeding bolts. The raw material smashing device is reasonable in structure, raw materials fall into the smashing assembly in the material bin, the falling raw materials are smashed through the smashing shaft, the granularity of the raw materials is uniform after the raw materials are smashed, the surface area of the raw materials is increased, attachment and reproduction of microorganisms are facilitated, the fixing buckle is opened on the fixing base, and the discharging opening in the conveying bin is placed in the fixing base. The fixing buckles are rotationally closed on the fixing seats to fix the conveying bin, the crushed raw materials fall into the conveying bin, the crushed raw materials can be transported into the culture bin for subsequent work, the conveying bin and the culture bin are rapidly separated, repair and maintenance of the conveying bin and the culture bin are facilitated, and the raw materials are convenient to transport.

The utility model discloses a coarse cereal bread cream compounding device adopting mixed fermentation of saccharomycetes and lactic acid bacteria, and relates to the technical field of food processing. The device comprises a mixing barrel, a top cover and a liquid pump, a defoaming net is clamped and fixed at the top in the mixing barrel, the top cover is clamped at the top of the mixing barrel, a stirring motor is clamped at the top of the top cover, the liquid pump is arranged on one side of the bottom of the mixing barrel, a return pipe is arranged on one side of the liquid pump, and a semiconductor chilling plate is clamped and fixed on the peripheral surface of the return pipe. Through the arrangement of the mixing barrel, the top cover and the liquid pump, the problems that when a compounding device is used for compounding cream, the cream is heated due to friction, the activity of saccharomycetes and the like is possibly damaged, the cream is deteriorated, the compounding device and a cream whipping device need to be arranged in a production workshop at the same time, the production cost is increased, and the production efficiency is increased are solved. The cream is easy to whip during stirring, and the whipped cream is difficult to mix with the flour quickly.

The invention relates to the technical field of fermentation of medicinal material production, and discloses a preservation method of a pseudo-ginseng cell strain, which comprises the following steps: acquiring a working cell strain; obtaining a liquid culture medium; carrying out mixed culture on the working cell strain and the liquid culture medium, and dehydrating; and soaking the dehydrated biomass in a cryoprotectant, and then carrying out cryopreservation or refrigerated preservation. Through cryopreservation or refrigerated preservation of the mixed solution, the preserved working cell strain can be adopted for fermentation in real time according to the requirements of a user, the richness of the strain can be guaranteed, the preparation period of the seed solution is saved, multi-batch fermentation can be realized, and the yield is stable.

The invention relates to a DNA tetrahedron-DNA microcapsule and a preparation method and application thereof in detection of staphylococcus aureus, and the preparation method comprises the following steps: designing four complementary oligonucleotide chains (S1, S2, S3 and S4), modifying an FAM fluorophore at the 5'end of S2, and heating and annealing to form a stable DNA tetrahedron; the method comprises the following steps: mixing a DNA tetrahedron, a Na2CO3 solution and a CaCl2 solution, carrying out coprecipitation to obtain a CaCO3 precipitate entrapping the DNA tetrahedron, and carrying out alternate incubation with an HEPES solution (containing PAH) and a DNA solution and treatment with an EDTA solution to obtain the DNA tetrahedron-DNA microcapsule. Staphylococcus aureus is cultured under proper conditions; and carrying out mixed culture on the DNA tetrahedron-DNA microcapsule and staphylococcus aureus, and carrying out fluorescence detection. The method is simple to operate, does not need complex instruments, is relatively low in cost, and can realize high-sensitivity and rapid detection of staphylococcus aureus.

The invention discloses a nano silicon-graphene-bacterial cellulose composite aerogel and a preparation method thereof, the nano silicon-graphene-bacterial cellulose composite aerogel is formed by compounding nano silicon, graphene and bacterial cellulose, the preparation method adopts a membrane liquid interface culture method, and the preparation method mainly comprises the following steps: preparing a culture medium for preparing bacterial cellulose, and preparing a bacterial cellulose basement membrane; preparing a mixed culture medium solution of nano silicon / graphene / uninoculated culture medium with different volume ratios; the preparation method comprises the following steps: circularly spraying mixed culture media of nano silicon / graphene with different concentrations on a basement membrane for multiple times in a spraying form, and after each spraying, carrying out next spraying after the mixed culture media on the surface of the membrane are completely consumed, so as to prepare the nano silicon-graphene-bacterial cellulose composite aerogel. According to the invention, the defects of huge volume expansion of nano silicon and easy agglomeration and non-uniform dispersion of nano silicon and graphene are effectively inhibited, the preparation process is simple, the composite aerogel has good performance, and the technical field of compounding is expanded.

The application discloses a fish and turtle mixed breeding method, which comprises the following steps of pond reconstruction, anti-escape setting and pond cleaning and disinfection. In the pond reconstruction, a fish pond is changed into a mixed breeding pond with a depth of 0.8-2 m, and the pond slope is designed as a gentle slope with an inclination of 35-40 degrees. The soil at the bottom of the pond should have good water retention and air permeability, preferably clay or loam. In the anti-escape setting, an anti-escape wall is formed around the pond by iron sheets or wires, the anti-escape wall is buried in the ground by 10-15 cm, and the ground height of the anti-escape wall is 80-150 cm. In the pond cleaning and disinfection, quicklime is used for disinfection before hibernation, and the whole pond is sprayed and splashed with water according to the proportion of 30 kg per mu. The application belongs to the technical field of aquaculture, and specifically provides a fish and turtle mixed breeding method, in which soft-shelled turtles crawl on the pond bottom, stir the bottom mud, improve the bottom quality, and provide a better water environment for fish. The soft-shelled turtles eat some dead fish and part of miscellaneous fish in the fish pond, and the fish has almost the same color as wild fish, good taste and high selling price.

The invention belongs to the field of chemical analysis and detection, and particularly relates to a research method for real-time analysis of a molecular mechanism of gold nanoparticles against bacteria by nanoESI-MS. The method comprises the following steps: performing mixed culture on the gold nanoparticles and bacteria, performing centrifugation, collecting supernate, taking the supernate as a sample solution, and performing analysis by using nanoESI-MS to obtain spectrogram data; performing orthogonal partial least square discriminant analysis on the spectrogram data, and establishing a metabolic substance and sample category prediction model; screening differential metabolites based on the prediction model and projection variable importance; and constructing a correlation network diagram based on the differential metabolites, and analyzing the antibacterial mechanism of the gold nanoparticles. According to the method, analysis can be carried out without sacrificing the integrity of the sample, so that the defects caused by the use of an extracting agent in the traditional mass spectrometry technology are avoided, and the analysis efficiency and the coverage range are also improved.

The application discloses a large-specification seawater fry factory grading cultivation system and relates to the technical field of aquaculture. The system comprises a first-stage, a second-stage and a third-stage cultivation pool. The first-stage cultivation pool is connected with the second-stage cultivation pool through a conveying pipeline, and the second-stage cultivation pool is arranged in the third-stage pool. The first-stage pool is provided with an independent first circulating water filtration device and a monitoring unit, and the second-stage and third-stage pools share a second set of circulating water filtration and monitoring units. The monitoring units at all stages are integrated with growth detection, environmental regulation and disease early warning functions. The first-stage pool is used for cultivating newly hatched fry, and when the fry develops to the juvenile stage, the fry is automatically transferred to the second-stage pool through the conveying pipeline; the second-stage pool and the third-stage pool jointly form a mixed culture environment, and when the juvenile fish reaches the target specification, the cage door is opened to transfer the juvenile fish into the third-stage pool. Through the grading environmental control and the automatic transfer mechanism, the system realizes efficient cultivation of fry from fry to large-specification fry, and solves the problems of uneven growth and low sorting efficiency in traditional fry cultivation.