64 results about "Mixed culture" patented technology

The invention relates to a method for producing higher alcohols. The invention also relates to a reaction mixture and a method for producing at least one higher alcohol, said method comprising a reaction mixture comprising a mixed culture of a first and a second microorganism in an aqueous medium comprising carbon monoxide gas, wherein the first microorganism is an acetogenic microorganism capable of converting a carbon source to acetic acid and / or ethanol; and-the second microorganism is selected from the group consisting of Clostridium kluyveri capable of converting acetic acid and / or ethanol to form an acid, and C. carboxidivorans; wherein the first microorganism is further capable of converting the acid to the corresponding higher alcohol, and the higher alcohol comprises at least 6 carbon atoms.

The application belongs to the technical field of microbial fermentation, and particularly relates to a kind of cytolysis product of mixed bacteria fermentation and a preparation method. The cytolysis product is prepared by mixed bacteria fermentation of two kinds of aerobic probiotics, i.e. Lactobacillus plantarum and Aspergillus oryzae are used as fermentation strains to carry out mixed bacteria fermentation treatment on rice milk, and membrane filtration and column chromatography system are used for filtration and enrichment, so as to obtain a cytolysis fermentation product filtrate rich in kojic acid, the content of kojic acid is greater than 200 g / L, which is significantly improved compared with conventional process. The obtained cytolysis product has no bad smell and clear color, so that the beneficial function of kojic acid can be played only with a low addition amount in cosmetics, and the addition of odor masking and color correcting auxiliaries is reduced, and the cytolysis product can be used for preparing cosmetics with skin repair, whitening and other functions.

The invention relates to a method for producing a water environment protective agent by fermenting hermetia illucens larva slurry, and belongs to the technical field of microbial fermentation. According to the present invention, the hermetia illucens larvae are adopted as the main raw material, the main raw material is ground into the slurry through the colloid mill, the small amount of the carbon source is supplemented, the Phaffia rhodozyma CGMCC NO.29217, the Bacillus coagulans CGMCC NO.5233 and the Clostridium butyricum CGMCC NO.4729 are subjected to the mixed bacteria synergistic fermentation, and the produced product can be widely used in the aquaculture and other application scenarios. Fermented hermetia illucens larva serous fluid is rich in astaxanthin, a fermented product has obvious acid fragrance and no peculiar smell, the total number of probiotics is larger than or equal to 5.0 * 10 < 8 > cfu / g, the pH value is smaller than 4.50, and the content of small molecule active polypeptide (acid soluble protein) of the fermented product is increased by 2.5-3 times compared with that before fermentation. Bacillus subtilis CGMCC (B) 63501 is used as indicator bacteria to detect that the content of bacteriostatic active substances after fermentation of hermetia illucens larva serous fluid is larger than 1.0 g / kg, pathogenic bacteria such as salmonella, vibrio parahaemolyticus and vibrio cholerae are not detected, and normal-temperature storage can be achieved. The preparation method is low in production cost, easy to operate, safe and free of residues, and the economic benefits of aquaculture can be improved.

The application provides a solid substrate for soilless culture prepared by fermentation of coffee residue and mixed bacteria, wherein the mixed bacteria include yeast, bacillus subtilis and trichoderma viride; the weight ratio of the yeast, the bacillus subtilis and the trichoderma viride is 2:1:3. The solid substrate for soilless culture is rich in trace elements required by plant growth, has low bulk density, high water holding porosity, and is controllable. The solid substrate for soilless culture can effectively improve the plant growth environment, has disease resistance and insect resistance, and improves the survival rate of plants.

The invention discloses a fish and shellfish polyculture experiment system and a verification method, and the system comprises at least two groups of experiment units which operate independently, an intelligent water distribution unit, a water quality on-line monitoring unit, a waste automatic collection unit and a central control unit. Wherein the experiment module comprises a fish culture area, a shellfish culture area, an adjustable mainstream circulation channel and a bypass circulation loop. The verification method comprises the steps of system initialization and experiment grouping, biological putting and feeding calibration, breeding operation under a controlled condition, multi-processing-group synchronous monitoring and sampling and data analysis and efficiency comprehensive evaluation. According to the method, the experimental platform which can accurately simulate the material circulation process of the fish-shellfish polyculture system, realize synchronous comparison of multiple treatment groups and quantitatively evaluate the nutrient interstage material coupling mechanism is constructed, and a scientific basis is provided for an ecological culture mode with low emission and high resource utilization rate.

This invention relates to the field of microbial fermentation technology, specifically to a Monascus purpureus strain with strong stress resistance and high esterase production, and its applications. The Monascus purpureus strain is *Monascus purpureus* (…). Monascus ruber ZH01, deposited on October 20, 2025, at the China Center for Type Culture Collection (CCTCC), accession number: CCTCC NO:M 20252263. This *Monascus purpureus* strain exhibits strong resistance to adverse conditions and can grow normally in an environment with pH 3 and 25% ethanol. Its fermentation products, after being dried at 50°C for 24 hours, still maintain a higher survival rate compared to the control. Furthermore, it can produce high levels of esterifying enzymes in mixed culture media, with an esterification power of 165.8 mg / g·100h. During the fermentation of baijiu and vinegar, esterification generates lipids, enhancing the flavor of the beverages. The production of esterified red yeast rice using *Monascus purpureus* ZH01 of this invention has advantages such as short production cycle, low cost, and high esterification power.

The invention belongs to the technical field of environmental engineering and pollution treatment, and particularly relates to an ecological intensive mariculture tail water intelligent treatment system and method.The ecological intensive mariculture tail water intelligent treatment system comprises a pretreatment and solid waste recycling module, a primary purification module, a biological collaborative purification module and a deep purification module; the pretreatment and solid waste recycling module comprises a high-density sedimentation tank, the primary purification module comprises a microalgae open pond, the biological collaborative purification module comprises a mixed culture area, and the deep purification module comprises an artificial wetland area. An outlet in the upper end of the microalgae open pond is connected with an inlet in the upper end of the mixed culture area through a pipeline I; according to the system, a biological treatment mode is adopted, the inherent defect of a single method in the prior art is overcome, standard discharge of mariculture tail water is achieved, green, ecological and efficient development of the breeding industry is promoted, and the win-win situation of ecological environment protection and economic benefit improvement is achieved.

The utility model relates to the technical field of organic fertilizer mixed culture, and discloses a soil functional bacteria organic fertilizer mixed culture device which comprises a feeding bin, a feeding conveyor is arranged on one side of the feeding bin, and the bottom of the feeding bin is connected with a smashing assembly, a conveying bin, a conveying motor and an auger shaft through feeding bolts. The raw material smashing device is reasonable in structure, raw materials fall into the smashing assembly in the material bin, the falling raw materials are smashed through the smashing shaft, the granularity of the raw materials is uniform after the raw materials are smashed, the surface area of the raw materials is increased, attachment and reproduction of microorganisms are facilitated, the fixing buckle is opened on the fixing base, and the discharging opening in the conveying bin is placed in the fixing base. The fixing buckles are rotationally closed on the fixing seats to fix the conveying bin, the crushed raw materials fall into the conveying bin, the crushed raw materials can be transported into the culture bin for subsequent work, the conveying bin and the culture bin are rapidly separated, repair and maintenance of the conveying bin and the culture bin are facilitated, and the raw materials are convenient to transport.

The utility model discloses a coarse cereal bread cream compounding device adopting mixed fermentation of saccharomycetes and lactic acid bacteria, and relates to the technical field of food processing. The device comprises a mixing barrel, a top cover and a liquid pump, a defoaming net is clamped and fixed at the top in the mixing barrel, the top cover is clamped at the top of the mixing barrel, a stirring motor is clamped at the top of the top cover, the liquid pump is arranged on one side of the bottom of the mixing barrel, a return pipe is arranged on one side of the liquid pump, and a semiconductor chilling plate is clamped and fixed on the peripheral surface of the return pipe. Through the arrangement of the mixing barrel, the top cover and the liquid pump, the problems that when a compounding device is used for compounding cream, the cream is heated due to friction, the activity of saccharomycetes and the like is possibly damaged, the cream is deteriorated, the compounding device and a cream whipping device need to be arranged in a production workshop at the same time, the production cost is increased, and the production efficiency is increased are solved. The cream is easy to whip during stirring, and the whipped cream is difficult to mix with the flour quickly.

The invention relates to the technical field of fermentation of medicinal material production, and discloses a preservation method of a pseudo-ginseng cell strain, which comprises the following steps: acquiring a working cell strain; obtaining a liquid culture medium; carrying out mixed culture on the working cell strain and the liquid culture medium, and dehydrating; and soaking the dehydrated biomass in a cryoprotectant, and then carrying out cryopreservation or refrigerated preservation. Through cryopreservation or refrigerated preservation of the mixed solution, the preserved working cell strain can be adopted for fermentation in real time according to the requirements of a user, the richness of the strain can be guaranteed, the preparation period of the seed solution is saved, multi-batch fermentation can be realized, and the yield is stable.

The application discloses a fish and turtle mixed breeding method, which comprises the following steps of pond reconstruction, anti-escape setting and pond cleaning and disinfection. In the pond reconstruction, a fish pond is changed into a mixed breeding pond with a depth of 0.8-2 m, and the pond slope is designed as a gentle slope with an inclination of 35-40 degrees. The soil at the bottom of the pond should have good water retention and air permeability, preferably clay or loam. In the anti-escape setting, an anti-escape wall is formed around the pond by iron sheets or wires, the anti-escape wall is buried in the ground by 10-15 cm, and the ground height of the anti-escape wall is 80-150 cm. In the pond cleaning and disinfection, quicklime is used for disinfection before hibernation, and the whole pond is sprayed and splashed with water according to the proportion of 30 kg per mu. The application belongs to the technical field of aquaculture, and specifically provides a fish and turtle mixed breeding method, in which soft-shelled turtles crawl on the pond bottom, stir the bottom mud, improve the bottom quality, and provide a better water environment for fish. The soft-shelled turtles eat some dead fish and part of miscellaneous fish in the fish pond, and the fish has almost the same color as wild fish, good taste and high selling price.

The present application relates to a kind of secondary biochemical effluent ferrous iron synergistic mixed culture denitrification method, to the secondary biochemical effluent of urban sewage or industrial wastewater treatment plant, then enter the baffling denitrification reaction tank of iron shavings-pyrite composite filling and activated sludge floc, occur heterotrophic denitrification process, autotrophic denitrification process under the action of denitrifying bacteria;After treatment, effluent enters the center pipe of vertical flow sedimentation tank, is discharged after precipitation, and part of sludge is discharged as excess sludge.The present application utilizes the heterotrophic denitrification, Fe 0 And Fe 2+ Autotrophic denitrification and sulfur autotrophic denitrification maintain water acid-base balance, improve denitrifying bacteria abundance, promote sulfur, iron autotrophic denitrification bacteria and heterotrophic denitrification bacteria synergistic denitrification effect, so as to improve denitrification efficiency.The method of the present application is easy to operate and manage, and has low operating cost, which can effectively reduce sludge production.

The present invention relates to a culture medium composition containing sugars and amino acids derived from human skin, microorganisms of the genera Staphylococcus, Streptococcus, Cutibacterium, Corynebacterium, and Rothia, and a cosmetic composition containing their lysate or culture medium. The mixed culture medium of human skin-derived microorganisms contained in the cosmetic composition is cultured in the culture medium composition and exhibits a significantly superior skin-improving effect compared to each of the individual culture mediums.

The invention discloses a saccharomyces cerevisiae YSBT-1 which is suitable for fermenting Chinese wolfberry juice, is preserved in the general microbiological center of the China Committee for Culture Collection of Microorganisms, and has the preservation number of CGMCC No.34262. The saccharomyces cerevisiae YSBT-1 can be used for fermenting Chinese wolfberry juice, and can be used for fermenting Chinese wolfberry juice. The strain is separated from a naturally-fermented yak yogurt sample in Qinghai province, has the characteristic of obvious aroma production, and grows well in Chinese wolfberry juice. The bacterium is mixed with plant lactobacillus to inoculate the fermented Chinese wolfberry, and the obtained mixed fermented Chinese wolfberry post-prebiotics beverage is orange red, strong in fruity flavor, has the unique flavor of Chinese wolfberry, and is moderate in sour and sweet taste and sweet in aftertaste. In-vitro and in-vivo experimental results show that the mixed fermentation Chinese wolfberry post-generation beverage has a remarkable anti-oxidation effect. As an excellent fermentation strain, the saccharomyces cerevisiae YSBT-1 has good development and application prospects in the field of functional foods such as fermented fruits and vegetables.

The application discloses a kind of ginseng fermentation mixed culture ferment and application thereof.Belongs to leisure food preparation technical field.The mixed culture ferment includes lactobacillus plantarum LNJ-HQ-01, Leuconostoc mesenteroides LNJ-L-014 and lactobacillus fermentum BNCC194390.The application also discloses a kind of preparation method of hawthorn ginseng leisure food, as follows: hawthorn is washed, cored;after ginseng powder is mixed with water, the mixed culture ferment of the application is used to ferment in dark and sealed;after hawthorn is treated, ginseng is mixed and microwave heating, beating, sieving, and fruit paste is made;fruit paste is mixed with syrup;after being pressed into sheet, microwave intermittent low-speed pulsating airflow drying is used.The beneficial effects obtained by the application are as follows:the mixed culture ferment in the application can effectively avoid bacterial contamination, improve the nutritional value of ginseng, improve the bitter taste of ginseng, and the hawthorn ginseng leisure food prepared by the mixed culture ferment has good nutritional value and palatability.

The application provides a multi-nutrition level mixed culture device for surface layer and bottom layer aquaculture species, which comprises four fixed piles, a fixed frame is fixedly installed between the top ends of the four fixed piles, longitudinal rods are fixedly arranged between the two sides of the fixed frame corresponding to the fixed piles, a transverse rod is fixedly arranged between the bottom ends of the left and right fixed piles, an adjusting mechanism is arranged on one side of the diagonal fixed piles, and a second lifting frame and a first lifting frame are arranged between the four fixed piles from bottom to top through the adjusting mechanism. The marine space can be fully utilized, the utilization rate of feed is improved, the wind and wave resistance is stronger, and the ecological culture of the surface layer and bottom layer aquaculture species is beneficial.

This invention relates to a method for treating high-concentration inorganic nitrogen wastewater using a mixed culture of *Paragonimus* strains wg2 and wg1, belonging to the field of resource and environmental microbiology. This invention utilizes the co-culture of strains wg2 and wg1 to treat simulated wastewater, primarily exploring the effect of the mixed strains wg2 and wg1 on NO2 under anoxic conditions. ‑ -N, NO3 ‑ The study investigated the nitrogen removal capacity of nitrite-nitrogen (N), the optimal ratio of mixed bacteria, and the nitrogen removal performance of the mixed bacteria in treating simulated wastewater. Results showed that *Paragonimus* strains wg2 and wg1 acted synergistically in a mixed culture system, effectively addressing both nitrite accumulation and efficient nitrate degradation, demonstrating high nitrogen removal capacity. This study provides important practical guidance for the development of biological nitrogen-removing agents that simultaneously remove nitrite and nitrate.

The utility model relates to aquatic products breeding equipment technical field especially discloses a kind of breeding ponds for live fish, including pool body, partition mechanism includes connecting plate and hole plate, each fixed mechanism includes limiting slot, connecting hole, plugboard one and plugboard two, the outside of each screw rod is connected with nut, the outside of hole plate is fixedly installed with two long plates, long plate sliding connection is in the outside of screw rod, a little water is placed in pool, first one fish is placed in pool body, the connecting hole of hole plate is aligned with limiting slot, plugboard one and plugboard two are inserted into the inside of connecting hole and limiting slot, at this time hole plate position is fixed, moving connecting plate makes screw rod and long plate connect, then nut is connected with the outside of screw rod, at this time connecting plate and hole plate connection are completed, at this time hole plate and connecting plate divide the space inside pool body into two layers, avoid different varieties of fish mixed culture fight and damage, reduce site and equipment repeated investment, reduce comprehensive breeding cost.

This invention consists of a process for producing bacteria and yeasts from agave sap and juice and honey, and using them as synbiotics in various functional beverages, including some tea-like liquid yogurt beverages. One process involves mixed culture to prepare a functional beverage with synbiotic action, comprising the combination of at least one yeast and one lactic acid bacterium with probiotic function, in a prebiotic solution of natural origin based on oligo- and polysaccharides from agave or honey, or both, for human consumption. The functional beverages described may contain alcohol in low concentrations or be completely alcohol-free. Another described process establishes the separate use of lactic acid bacteria and yeasts to generate fermented alcoholic, acidic, or both types of beverages, with or without live microorganisms, possessing high organoleptic quality.It also establishes the process by which a freeze-dried powder can be prepared, either individually or through a mixture of strains with probiotic activity, from honey or agave syrups.

The application discloses a method for improving autotrophic denitrification contribution of a mixed culture type mainstream anaerobic ammonia oxidation granular sludge process, and comprises the following steps: 1) performing carbon control pretreatment on municipal sewage, regulating and controlling the content of dissolved organic matter in the sewage and the proportion of the dissolved organic matter and total nitrogen, and improving the carbon source quality of the sewage; 2) performing limited oxygen aeration treatment on part of the sewage after the carbon control pretreatment, mixing the sewage with the sewage without aeration treatment to optimize the nitrogen composition of the sewage, and then pumping the sewage into an upflow anaerobic ammonia oxidation reactor; and 3) limiting the heterotrophic denitrifying bacteria mediated nitrite nitrogen reduction activity in the upflow anaerobic ammonia oxidation granular sludge reactor through directional sludge discharge based on microsieve screening. According to the method, the autotrophic denitrification contribution of the anaerobic ammonia oxidation granular sludge reactor can be improved to more than 75%, and the effluent meets the first level A discharge standard of urban sewage, and the method has the advantages of flexibility, high efficiency and wide applicability.

The invention discloses a microalgae polyculture method for adjusting supply of an organic carbon source through dissolved oxygen content feedback and application, and belongs to the technical field of microalgae culture, the method comprises the following steps: an autotrophic stage: introducing air into a culture reactor, and enabling cells to reach a certain concentration through autotrophic growth; in the polyculture stage, ventilation is stopped, dissolved oxygen is monitored in real time, starting and stopping of an organic carbon source pump are dynamically adjusted, and mixed nutrition is achieved; and continuous production: setting a dilution rate, continuously harvesting, supplementing the culture medium, and continuously operating. The addition of an organic carbon source is accurately controlled through the concentration feedback of dissolved oxygen, the photosynthetic and heterotrophic metabolism rates are matched, no net oxygen production (stable DO) is ensured, oxygen self-balance in the system is realized, and finally, the conversion rate of carbon is greatly increased and can reach 0.85 or above. According to the method, the microalgae productivity is remarkably improved under the condition of no gas exchange, and a new thought is provided for large-scale culture with low energy consumption and high carbon efficiency.

The application provides a kind of cluster root fungus agent for improving the cold resistance of durian and a preparation method thereof, and belongs to the technical field of microbial inoculant.The preparation method of the cluster root fungus agent comprises the following steps: collecting subfrigid zone plant rhizosphere soil, and screening cluster root fungus spores in the soil;inoculating the cluster root fungus spores into sand mixed culture medium, then planting white clover or ryegrass and other plants to carry out enrichment culture and obtain culture;mixing the culture with cutting substrate, and cutting one-year-old durian branches to carry out rooting culture and first low-temperature culture;storing the root system of cutting seedlings in cold storage, crushing, mixing with cutting substrate, cutting one-year-old durian branches to carry out second low-temperature culture, and collecting the root system of cutting seedlings and rhizosphere soil to obtain the cluster root fungus agent.The cluster root fungus agent can induce durian to produce low-temperature resistant protective enzymes, thereby improving the cold resistance of durian and providing a new idea for cultivating durian cold-resistant varieties.

The invention provides an improved material based on microorganism and mineral synergy and a preparation method and application thereof, the application comprises a method for improving saline-alkali soil by using the improved material, and the method comprises the following steps: carrying out water bath shaking culture on a thiobacillus ferrooxidans solution to obtain a thiobacillus ferrooxidans microbial inoculum; the method comprises the following steps: removing impurities from high-sulfur coal gangue, air-drying, crushing and grinding to obtain coal gangue powder; mixing the coal gangue powder with water, inoculating the mixture with a thiobacillus ferrooxidans microbial inoculum for culturing, and introducing fungi to obtain biologically activated coal gangue; determining the application mass ratio and the total application amount of the bioactive coal gangue and the low-sulfur coal gangue; mixing the bioactive coal gangue with low-sulfur coal gangue to obtain an improved material; pretreating the soil to obtain a saline-alkali soil sample; performing mixed culture on the improved material and the saline-alkali soil sample, and verifying effectiveness; and after verification, applying the improvement material to the target saline-alkali soil. According to the method, the high-sulfur coal gangue is activated by using a microbial technology, the saline-alkali soil improvement efficiency is improved, and waste is turned into wealth.

The application discloses a method for synthesizing lactic acid from lignocellulose by using a fungus-bacterium mixed culture system with functional complementation, and comprises the following steps: (1) inoculating activated Trichoderma asperellum into a fermentation medium containing microcrystalline cellulose to perform fermentation, and obtaining a fermentation liquor; and (2) inoculating activated Lactobacillus paracasei into the fermentation liquor obtained in the step (1) to perform anaerobic fermentation and produce lactic acid. When the Lactobacillus paracasei is inoculated when the Trichoderma asperellum is cultured for 40 hours, the highest lactic acid yield is achieved, and reaches 57.59 g / L. The method is currently used to obtain the highest lactic acid yield in the mixed culture fermentation by using microcrystalline cellulose as the only carbon source. Meanwhile, the method can also be applied to a fermentation process for directly synthesizing lactic acid from lignocellulose. When the fermentation medium with corn cobs as the substrate is used to perform fermentation, 9.80 g / L of lactic acid can be produced, the substrate cost for industrial production of lactic acid is greatly reduced, and the method has important application value.

The invention discloses a membrane coupling type microalgae reactor in the field of sewage treatment, and the membrane coupling type microalgae reactor comprises a water supply assembly used for supplying to-be-treated sewage; the first reactor is communicated with the water outlet of the water supply assembly, the first reactor comprises a rotatable biological membrane structure and an aeration structure, and the sewage is primarily treated through a biological membrane; the second reactor is communicated with the first reactor; the ceramic membrane separation unit is communicated with the second reactor and is used for carrying out cross-flow filtration on sewage discharged from the second reactor so as to realize collection of a biological membrane and microalgae and conveying the biological membrane back into the first reactor; the invention further discloses a sewage treatment method. The sewage treatment device has the beneficial effects that microalgae growth and biomass accumulation can be enhanced through two-stage mixed culture of the first reaction cylinder body and the second reaction cylinder body, illumination of the biological modified filler is more uniform, the sewage treatment performance is improved, and the device is lower in energy consumption.

This invention relates to the field of aquaculture engineering technology, and discloses a gravity-based three-dimensional polyculture system for fish, shellfish, and algae based on intelligent monitoring, and its control method. The system includes a polyculture system net, which is used to optimize ecological efficiency when fish, shellfish, and algae are polycultured. The net contains polyculture support components. In this invention, the polyculture system net enables three-dimensional, stratified polyculture of fish, shellfish, and algae, establishing a complete trophic cascade relationship. Fish uneaten feed and excrement are filtered and utilized by mid-layer shellfish, shellfish metabolites promote the growth of bottom-layer algae, and algae photosynthesis oxygenates the system and absorbs eutrophic salts, forming a closed-loop, highly efficient ecological cycle. Combined with dissolved oxygen sensors, flow meters, and multi-dimensional water quality sensors for collaborative monitoring, the culture density at each layer can be precisely controlled, increasing the system's material conversion efficiency by more than 30% and reducing feed input by 25% compared to traditional monoculture models.

A method for degrading and reusing discarded cellulose products, including using recycled waste cellulose products and fermenting them with a mixed culture of Trichoderma viride, Trichoderma reesei, Aspergillus niger, and Trichoderma pseudokoningii to obtain crude cellulase products.

The invention discloses a construction method and application of carbon quantum dot-pseudomonas stutzeri engineering bacteria, and belongs to the technical field of crossing of agricultural microorganisms and nano materials. The preparation method comprises the following steps: carrying out gene modification on wild nitrogen-fixing pseudomonas stutzeri A1501 to construct an engineering bacterium A1501 / pLA-nifA over-expressed by a nitrogen-fixing key positive regulation factor nifA, synthesizing iron-nitrogen co-doped carbon quantum dots Fe, N-CDs with efficient electron transfer capability through a one-step hydrothermal method, and carrying out mixed culture on the Fe, N-CDs and the A1501 / pLA-nifA under proper conditions to construct the Fe, N-co-doped carbon quantum dots Fe, N-CDs. According to the N-CDs-A1501 / pLA-nifA hybrid system, photoelectrons generated by photoelectric properties of Fe and N-CDs are used as electron donors, so that the CD-ROM nitrogen fixation efficiency of the N-CDs-A1501 / pLA-nifA hybrid system is enhanced. The constructed Fe, N-CDs-A1501 / pLA-nifA hybrid system can significantly improve the yield of NH4 < + >-N of nitrogen-fixing bacteria, and compared with P.s A1501, the yield of NH4 < + >-N can reach 210.08 mu g / mL and is improved by 3.33 times. The invention provides a new effective way for developing an efficient and green biological nitrogen fixation technology, and has a wide application prospect in the fields of agricultural microbial fertilizers and environmental restoration.

The present application belongs to the field of biotechnology, and particularly relates to a method for improving accumulation of fucoxanthin, oleic acid and arachidonic acid in brown flagellate culture. The method is to add microcystis to brown flagellate for mixed culture, and continuously aerate, and the microcystis is phagocytosed by the brown flagellate. The method of phagocytosis of microcystis by brown flagellate is used to realize higher accumulation of fucoxanthin in brown flagellate. Through the experiment, it is proved that the brown flagellate has higher removal efficiency for non-toxic microcystis, and can also effectively ingest toxic microcystis, and a new way for obtaining higher accumulation of fucoxanthin in biological manufacturing is provided.