33 results about "Phaffia rhodozyma" patented technology

The invention discloses a mesophilic carotenoid-producing recombinant yarrowia lipolytica strain as well as a construction method and application thereof, the recombinant strain exogenously expresses mesophilic original fatty acid desaturase (FAD3) and isocitrate dehydrogenase (IDH2) mutants from phaffia rhodozyma, and efficient synthesis under the condition of 30 DEG C is realized; secondly, a metabolic pathway of the recombinant strain is optimized based on a metabolic flow push-pull strategy, and isopentenyl pyrophosphate isomerase (IDI), farnesyl pyrophosphate synthase (ERG20), plant alkene dehydrogenase (CarB) and bifunctional plant alkene synthase (CarRP) are overexpressed, so that the flux of a precursor is increased; finally, gene multi-copy of astaxanthin synthase (crtS) and cytochrome P450 reductase (crtR) from phaffia rhodozyma is carried out, and the carotenoid production capacity of yarrowia lipolytica is further improved. The construction method of the mesophilic yarrowia lipolytica is simple, efficient synthesis of carotenoid can be better achieved at the higher temperature, the fermentation cost is greatly reduced, and the industrial production potential is achieved.

The invention provides a method for promoting phaffia rhodozyma to produce astaxanthin, and belongs to the technical field of microbial fermentation. The invention provides a phaffia rhodozyma LX6, the phaffia rhodozyma LX6 is preserved in the China Center for Type Culture Collection (CCTCC), the preservation number is CCTCC No. M 20242814, the phaffia rhodozyma LX6 is classified and named as the phaffia rhodozyma LX6, and the preservation time is December 16, 2024. The phaffia rhodozyma LX6 is named as the phaffia rhodozyma LX6. According to the fermentation method for improving the yield of the astaxanthin, provided by the invention, the yield of the biologically produced astaxanthin is promoted to be improved, the astaxanthin extracted by fermentation can be used for feeds, health-care products, cosmetics and the like, and the extracted thalli can be used as proteins to be added into animal feeds, so that the quality of the feeds is improved.

The invention provides a phaffia rhodozyma mutant strain for high yield of astaxanthin as well as a screening method and application of the phaffia rhodozyma mutant strain, belongs to the technical field of fermentation microorganism screening, and can solve the problems of low astaxanthin yield, high cost and low growth temperature of wild type phaffia rhodozyma existing in synthesis of astaxanthin from a phaffia rhodozyma strain. The invention provides a phaffia rhodozyma mutant strain capable of producing astaxanthin at high yield, the phaffia rhodozyma mutant strain is a phaffia rhodozyma AA0529 mutant strain, the phaffia rhodozyma AA0529 mutant strain is preserved in China Center for Type Culture Collection in Wuhan, Hubei on September 7, 2023, and the preservation number is CCTCC (China Center for Type Culture Collection) NO: M20231637. According to the method, the yield of the astaxanthin can be increased, and the yield of the astaxanthin is increased to 3.3 mg / g at 26 DEG C.

The invention discloses a fermented feed prepared from traditional Chinese medicine residues as well as a preparation method and application of the fermented feed. The fermented feed is prepared by mixing and fermenting a mixed bacteria solution formed by fermenting bacillus subtilis, bacillus coagulans, phaffia rhodozyma and lactobacillus plantarum with bran, soybean meal, rice husks, zeolite powder and honeysuckle dregs. The fermented feed disclosed by the invention increases the contents of microbial protein, lactic acid, ethanol, tartaric acid, acetic acid and lactic acid bacteria, contains various fermentation substances, can improve the appetite of animals and improve the palatability, and is high in nutritional value. Honeysuckle herb residues are used as main raw materials, so that the preparation method has the characteristic of low production cost, and the problem of environmental pollution caused by random discarding of herb residue wastes is solved.

The invention discloses a method for extracting astaxanthin from phaffia rhodozyma by combining bacillus fermentation with ionic liquid. The method comprises the following steps: carrying out co-culture on bacillus velezensis Y12 and phaffia rhodozyma, so as to obtain a fermentation solution; mixing the fermentation liquor with ionic liquid in equal volume, stirring, centrifugally separating out the ionic liquid containing astaxanthin, and finally synthesizing the novel astaxanthin emulsion with water and sunflower seed oil. The preservation number of the bacillus velezensis Y12 is CGMCC (China General Microbiological Culture Collection Center) No.27125, and the bacillus velezensis Y12 can be used for preparing the bacillus velezensis. According to the method, the extraction rate of the phaffia rhodozyma astaxanthin is remarkably increased, meanwhile, the antioxidant activity and stability of the astaxanthin are effectively improved, and the method has important industrial application value.

The invention relates to a method for producing a water environment protective agent by fermenting hermetia illucens larva slurry, and belongs to the technical field of microbial fermentation. According to the present invention, the hermetia illucens larvae are adopted as the main raw material, the main raw material is ground into the slurry through the colloid mill, the small amount of the carbon source is supplemented, the Phaffia rhodozyma CGMCC NO.29217, the Bacillus coagulans CGMCC NO.5233 and the Clostridium butyricum CGMCC NO.4729 are subjected to the mixed bacteria synergistic fermentation, and the produced product can be widely used in the aquaculture and other application scenarios. Fermented hermetia illucens larva serous fluid is rich in astaxanthin, a fermented product has obvious acid fragrance and no peculiar smell, the total number of probiotics is larger than or equal to 5.0 * 10 < 8 > cfu / g, the pH value is smaller than 4.50, and the content of small molecule active polypeptide (acid soluble protein) of the fermented product is increased by 2.5-3 times compared with that before fermentation. Bacillus subtilis CGMCC (B) 63501 is used as indicator bacteria to detect that the content of bacteriostatic active substances after fermentation of hermetia illucens larva serous fluid is larger than 1.0 g / kg, pathogenic bacteria such as salmonella, vibrio parahaemolyticus and vibrio cholerae are not detected, and normal-temperature storage can be achieved. The preparation method is low in production cost, easy to operate, safe and free of residues, and the economic benefits of aquaculture can be improved.

The invention relates to a method for producing astaxanthin through fermentation of phaffia rhodozyma, and belongs to the technical field of microbial fermentation. The invention provides a method for producing astaxanthin by fermenting phaffia rhodozyma, which specifically comprises the following steps: inoculating an activated phaffia rhodozyma single strain into a seed culture medium, culturing to obtain a primary seed solution, inoculating the primary seed solution into the seed culture medium, culturing to obtain a secondary seed solution, inoculating into a fermentation culture medium, and fermenting at 20 DEG C to obtain the astaxanthin. Centrifuging, discarding supernatant, cleaning thalli with normal saline, performing wall breaking to obtain wall-broken liquid, and extracting to obtain astaxanthin. According to the method, seed culture is performed twice to improve the strain activity and enhance the metabolic activity, fermentation is performed on this basis to promote the synthesis and stability of astaxanthin, so that the phaffia rhodozyma thalli with higher astaxanthin content are obtained, and the phaffia rhodozyma thalli are prepared into liquid preparations or dry powder preparations to be applied to aquaculture.

The invention relates to the technical field of microorganisms, in particular to a method for producing astaxanthin emulsion from phaffia rhodozyma, which specifically comprises the following steps of: performing superfine grinding on dried phaffia rhodozyma, extracting astaxanthin by using a solvent, dissolving the astaxanthin in soybean oil, taking whey protein as a water phase, preparing O / W emulsion, adding tannic acid to protect the astaxanthin from being influenced by enzymatic crosslinking, and preparing the astaxanthin emulsion by using the tannic acid. And a'multilayer 'interfacial film is formed through electrostatic assembly and enzymatic crosslinking. The chitosan-sodium alginate'multi-layer 'interfacial film improves the mechanical strength of the interfacial film, and limits the oxidizing agent from permeating into the internal oil phase to be in contact with the astaxanthin. Flocculation of the astaxanthin emulsion is inhibited through the charge neutralization effect of sodium alginate grafted polyethylene glycol sheltered salt ions, enzymatic crosslinking is conducted on chitosan grafted peptide fragments, and targeted release of the astaxanthin in the intestinal tract is achieved. The use amount of tyrosinase and the enzymatic reaction time are controlled, the residue of tyrosinase with oxidizing property is prevented, and tannic acid prevents the oxidizing substance generated by the reaction of tyrosinase from oxidizing astaxanthin.

The invention discloses a phaffia rhodozyma strain with high astaxanthin yield and application of the phaffia rhodozyma strain. The phaffia rhodozyma strain capable of producing the astaxanthin at the high yield is named as phaffia rhodozyma CL17, and is preserved in the China General Microbiological Culture Collection Center (CGMCC) on October 21, 2025, and the preservation number is CGMCC No.39010. The phaffia rhodozyma strain can be used for producing the astaxanthin at the high yield. The strain obtained through screening has good temperature tolerance (15-45 DEG C) and hereditary stability, after fermentation culture, the astaxanthin content in thalli reaches 5.13 mg / g of dry cell weight, and the strain has excellent application prospects.

The invention belongs to the technical field of molecular biology and biosynthesis, and provides a method for increasing the yield of astaxanthin in phaffia rhodozyma through transcription factors. WC1 and / or WC2 are / is overexpressed in the phaffia rhodozyma. According to the method, WC1 and WC1-WC2 overexpression engineering strains are constructed, so that innovative application of transcription factor engineering in construction of phaffia rhodozyma high-yield strains is realized, the biological synthesis capacity of astaxanthin is remarkably improved, and a new technical approach is provided for metabolic engineering modification of phaffia rhodozyma.

The present application relates to the technical field of chemical preparations, in particular to a method for improving the bioavailability of astaxanthin in phaffia rhodozyma powder and application. The present application uses composite enzymes to pretreat phaffia rhodozyma powder, so that astaxanthin is fully released from the cells, and then specially-made eutectic solvent is used to efficiently extract the pretreated phaffia rhodozyma powder, and the extraction rate can reach more than 90%. The astaxanthin extract prepared by the present application has high antioxidant activity and high bioavailability, and the DPPH free radical scavenging rate can be up to 89.14%, the ABTS free radical scavenging rate can be up to 90.09%, and the bioavailability can be up to 81.41%. In addition, the present application is simple to operate, does not need ultrasonic, microwave and other assisted methods, has low requirements on equipment, can be suitable for industrial large-scale production, provides a feasible scheme for the green and efficient extraction of astaxanthin, and has a wide application prospect in the food, cosmetic and feed industries.

The invention discloses a method for increasing the yield of astaxanthin in phaffia rhodozyma by using an iron reductase gene. Firstly, the invention relates to application of an iron reductase gene in improving the yield of astaxanthin in phaffia rhodozyma; the method comprises the following steps: introducing an iron reductase gene into phaffia rhodozyma cells, independently expressing the iron reductase gene, and carrying out fermentation treatment by using phaffia rhodozyma to produce astaxanthin. According to the invention, the iron reductase gene is independently overexpressed in phaffia rhodozyma cells, so that the biomass of phaffia rhodozyma and the yield of astaxanthin are improved; the phaffia rhodozyma high-yield astaxanthin strain is constructed through a gene overexpression technology, has the characteristics of genetic characters and stable astaxanthin fermentation yield, and is relatively high in feasibility.

The invention discloses a zymophyte enzyme preparation containing phaffia rhodozyma and lactobacillus acidophilus and application of the zymophyte enzyme preparation. The zymophyte enzyme preparation contains phaffia rhodozyma, lactobacillus acidophilus, alkaline protease and cellulase. The zymophyte enzyme preparation is added into the soybean meal, the fermented soybean meal is obtained through fermentation, the content of glutamic acid in the fermented soybean meal can be remarkably increased, the content of antigen protein in the soybean meal can be reduced, the fermented soybean meal replaces 30% by mass of soybean meal and is evenly mixed with corn flour and bran to be directly fed to fattening pigs, the total ammonia content in pork can be remarkably reduced, and the pig fattening efficiency is improved. The fishy smell of the pigs is reduced, the meat quality is improved, and the feed has good application value in fattening pig breeding.

The invention belongs to the field of feeds, and discloses a method for preparing a fermented feed based on bean dregs and shrimp shells and the fermented feed, and the method comprises the following steps: step 1, preparing bean dregs, folium artemisiae argyi hay meal, corn, corn steep liquor and shrimp shell meal into a fermentation substrate; step 2, inoculating a fermentation substrate with phaffia rhodozyma, and carrying out solid-state fermentation culture in an aerobic environment; and step 3, drying the fermentation product to prepare fermentation powder. According to the method, bean dregs serve as a main material and a nitrogen source, corn and corn steep liquor serve as a carbon source, folium artemisiae argyi and shrimp shell meal are used for increasing the content of flavone, polysaccharide and astaxanthin of the feed, improving the immunocompetence of animals and improving intestinal health, bean dreg fermentation products can be better utilized, and replacement of staple food grains by the fermentation products is truly achieved; the fermented feed disclosed by the invention is low in antigen protein content and low in poor oligosaccharide content, and is beneficial to digestion and utilization of bean dregs by animals.

The invention discloses a method for improving the yield of astaxanthin synthesized by microorganisms through double-stage regulation and control by utilizing red / blue light illumination. Red light (RGB: 255, 0, 0) and blue light (RGB: 0, 0, 255) are selected as illumination sources, the illumination intensity of the red light is 1500-2000 Lux, and the illumination intensity of the blue light is 2500-3000 Lux. Red light irradiation is started for two days on the first day of phaffia rhodozyma fermentation, blue light is converted into blue light on the third day of fermentation, and after illumination is conducted for five days, the content and yield of astaxanthin are increased by 176.33% and 151.78% respectively. The research provides a simple and economic regulation and control method for efficient synthesis of astaxanthin from phaffia rhodozyma.

This invention discloses a composition that helps maintain healthy blood lipid levels and its application, belonging to the field of health product technology. The composition comprises 1-3 parts rice bran fatty alkyl alcohol, 0.5-1.5 parts Torreya grandis extract, and 5-7 parts compound fermentation extract. The compound fermentation extract is prepared by fermenting a mixture of sea cucumber extract and fig concentrate powder using a specific preserved strain of Phaffia rhodozyma D312 (CCTCC NO: M 20232149). This invention achieves comprehensive blood lipid regulation through the synergistic effect of the fermentation strain and the fermentation substrate. Furthermore, by scientifically combining it with rice bran fatty alkyl alcohol and Torreya grandis extract, the three components synergistically regulate blood lipid metabolism through different mechanisms of action. The blood lipid-lowering effect of the composition is significantly better than that of any single component or any combination of two components. All raw materials in this invention are of natural origin, and the maximum tolerated dose experiment in mice has proven its good safety profile, making it suitable for long-term use. It has broad application prospects in the preparation of health products with the function of maintaining healthy blood lipid levels.

The invention discloses a method for increasing the yield of astaxanthin in phaffia rhodozyma by using a synergistic gene expression strategy. Firstly, the invention relates to application of a non-homologous terminal connection protein gene and an iron reductase gene in improving the yield of astaxanthin in phaffia rhodozyma; the method comprises the following steps: introducing a non-homologous terminal junction protein gene and an iron reductase gene into phaffia rhodozyma cells, cooperatively expressing the non-homologous terminal junction protein gene and the iron reductase gene, and fermenting by using the phaffia rhodozyma to produce the astaxanthin. According to the method disclosed by the invention, the biomass and the astaxanthin yield of the phaffia rhodozyma are improved by synergistically overexpressing the non-homologous terminal connecting protein and the iron reductase gene in phaffia rhodozyma cells; the phaffia rhodozyma high-yield astaxanthin strain is constructed through a gene overexpression technology, has the characteristics of genetic characters and stable astaxanthin fermentation yield, and is relatively high in feasibility.

The application discloses a strain of Scheffersomyces fibuliger and a microbial agent and application thereof, and belongs to the field of biology.The Scheffersomyces fibuliger (Scheffersomyces fibuliger) Cyberlindnera fabianii ) LZLJ1-6 has a preservation number of CCTCC NO: M 2025590. Pure-strain fermentation of the strain can metabolically produce a plurality of flavor substances such as secondary octanone, nonanal and heptyl alcohol. Application of a microbial agent containing the strain to rice wine fermentation can significantly increase the richness of flavor substances in the rice wine, including a plurality of alcohol, ester, ketone, acid and phenolic compounds, and meanwhile, the content of some original flavor substances is increased. The strain provided by the application has characteristics in flavor metabolism, is particularly outstanding in synthesis of characteristic aroma components such as secondary octanone, and has important significance for enriching flavor levels and improving aroma complexity in wine production, especially rice wine fermentation, and also provides high-quality strain resources for development of new fermented foods.

The invention belongs to the field of microbial engineering, and discloses a wall-broken phaffia rhodozyma feed additive as well as a preparation method and application thereof. The preparation method of the wall-broken phaffia rhodozyma feed additive comprises the following steps: strain culture: inoculating phaffia rhodozyma into a YM liquid culture medium, and culturing to obtain a seed solution; fermenting and expanding culture: transferring the seed solution into a new YM liquid culture medium, fermenting and culturing, and centrifuging to obtain a thallus precipitate; wall breaking treatment: adding hydrochloric acid into the thallus precipitate, heating, cooling, and centrifuging to obtain a precipitate; and freezing and drying to obtain the wall-broken phaffia rhodozyma feed additive. By adopting the wall-broken phaffia rhodozyma feed additive as well as the preparation method and the application thereof, the growth rate and the survival rate of offspring seeds can be remarkably increased, meanwhile, the oxidative stress resistance and the immunity of the offspring seeds are improved, and the stress resistance of the offspring seeds is enhanced.

The invention discloses a screening method and application of phaffia rhodozyma strains. The phaffia rhodozyma strain HSASTA is obtained through screening evolution and has characteristic sequences of five key enzyme genes in the anabolism process of astaxanthin. When the phaffia rhodozyma is used for producing the astaxanthin, the yield can reach 8000 mg / kg or above. The strain can be applied to various industrial fields of health care products, food, food additives and the like, and has wide development and application prospects.

The invention discloses a method for increasing the yield of astaxanthin in phaffia rhodozyma by using a non-homologous terminal junction protein gene. Firstly, the invention relates to application of a non-homologous terminal connection protein gene in improving the yield of astaxanthin in phaffia rhodozyma; according to the method, non-homologous terminal connexin genes are introduced into phaffia rhodozyma cells, non-homologous terminal connexin is independently expressed, and then fermentation treatment is performed by using phaffia rhodozyma to produce astaxanthin. According to the method disclosed by the invention, the biomass of phaffia rhodozyma and the yield of astaxanthin are improved by independently overexpressing the non-homologous terminal junction protein gene in phaffia rhodozyma cells; the phaffia rhodozyma high-yield astaxanthin strain is constructed through a gene overexpression technology, has the characteristics of genetic characters and stable astaxanthin fermentation yield, and is relatively high in feasibility.

The invention relates to the technical field of edible fungus strain breeding, in particular to phaffia rhodozyma capable of producing astaxanthin at high yield and application of the phaffia rhodozyma. The phaffia rhodozyma HFF5011 provided by the invention is preserved in the China Center for Type Culture Collection on December 16, 2024, and the preservation number of the phaffia rhodozyma HFF5011 is CCTCC (China Center for Type Culture Collection) NO: M 20242822. The strain is obtained by domestication and breeding on the basis of phaffia rhodozyma (named as phaffia rhodozyma HFF501) with the preservation number of CCTCC (China Center For Type Culture Collection) NO: M 2020413. The phaffia rhodozyma provided by the invention is a strain with higher astaxanthin yield screened by utilizing a beta-violone culture medium on the basis of an original strain, and the content of astaxanthin fermented by the strain can be up to 630mg / L.

The utility model relates to the technical field of phaffia rhodozyma fermentation, and discloses a fermentation device for phaffia rhodozyma production, which comprises a support, the upper part of the support is fixedly connected with a fermentation barrel, the upper part of the fermentation barrel is provided with a barrel cover, and the right side of the upper part of the barrel cover is fixedly connected with a feed port; mounting grooves are formed in the two sides of the exterior of the feeding opening, mounting assemblies are arranged in the two mounting grooves, a mounting shell is mounted on the upper portion of the feeding opening, a filter screen is mounted in the mounting shell, and a mixing assembly is arranged on the upper portion of the barrel cover; the mounting assembly comprises a first spring, and the first spring is fixedly connected to the interiors of the two mounting grooves. According to the phaffia rhodozyma fermentation tank, before the phaffia rhodozyma is conveyed into the fermentation barrel, the phaffia rhodozyma can be conveniently filtered, dust in air or impurities in materials are prevented from entering the fermentation tank together with the phaffia rhodozyma, and therefore the quality of the phaffia rhodozyma is improved.

The invention belongs to the technical field of meal replacement food, and particularly relates to nutritional meal replacement powder after liposuction based on microbial fermentation and a production method thereof, the product mainly comprises a microbial fermentation group, a grain group, a bean rhizome group and a nutrition enrichment group in parts by mass, and the component proportion is scientific and reasonable; various functional bacteria such as artificial cordyceps sinensis powder, rhodotorula crispa powder and monascus powder are introduced into a fermentation group, and the absorption and utilization rate of nutritional ingredients is increased in a solid-state fermentation mode. The gynostemma pentaphyllum component is beneficial to maintaining the pH value of a fermentation environment to be 4.5-6.0, the micro-ecological environment is optimized, and the postoperative intestinal function is improved. The preparation method comprises the steps of pretreatment, mixing, fermentation, drying, grinding, vacuum packaging and the like, the process is mild, and the activity of the raw materials is reserved. The meal replacement powder is rich in high-quality vegetable proteins, dietary fibers and various vitamin minerals, meets the requirements of postoperative energy supplement and tissue repair, has the advantages of balanced nutrition, high absorption rate, fine and smooth taste and the like, and is suitable for postoperative gastrointestinal sensitive people to eat.

The invention provides recombinant yarrowia lipolytica for co-production of microbial oil and astaxanthin as well as a construction method and application of the recombinant yarrowia lipolytica, and belongs to the technical field of genetic engineering. The recombinant yarrowia lipolytica expresses geranyl geranyl diphosphate synthase CrtE derived from phaffia rhodozyma, phytoene synthetase / lycopene cyclase CrtYB, phytoene desaturase CrtI, 3-hydroxy-3-methylglutaryl CoA reductase tHMGR derived from saccharomyces cerevisiae, 3-hydroxy-3-methylglutaryl CoA reductase tHMGR derived from saccharomyces cerevisiae, 3-hydroxy-3-methylglutaryl CoA reductase tHMGR derived from saccharomyces cerevisiae, and 3-hydroxy-3-methylglutaryl CoA reductase tHMGR derived from saccharomyces cerevisiae. The beta-carotene ketolase CrtW and the beta-carotene hydroxylase CrtZ are derived from the haematococcus pluvialis. According to the method, coproduction of the microbial oil and the astaxanthin by using the yarrowia lipolytica is realized by using the molasses as a cheap raw material in a 5L fermentation tank, the yield of the microbial oil reaches 80.14% and the yield of the astaxanthin reaches 3.09 g / L after fermentation is performed for 168 hours, and a foundation is laid for further industrialization.

The present invention discloses a thermotolerant rhodozyma strain and a method for producing astaxanthin using straw hydrolyzate. The strain is classified and named as rhodozyma GF6. Xanthophyllomyces dendrorhous GF6, with a deposit number of CCTCC NO: M 2025649; the strain is inoculated into a fermentation medium, and straw hydrolyzate is used as a carbon source to efficiently produce astaxanthin at 28-30°C; the present invention utilizes agricultural waste straw hydrolyzate as a cheap crude raw material instead of the entire carbon source to produce astaxanthin, thereby increasing the fermentation temperature, reducing production costs, and being environmentally friendly.

The invention relates to the technical field of animal husbandry, and particularly discloses a schizochytrium limacinum composition for regulating physiological indexes and DHA (docosahexaenoic acid) of dairy cows at high temperature in summer, the schizochytrium limacinum composition is obtained by melt mixing and cold spraying of yucca extract, oregano essential oil, vitamin E, phaffia rhodozyma powder, tea polyphenol, organic selenium and an oil phase mixture. Compared with single use of each component in the composition, the composition can significantly improve various physiological indexes of dairy cows, can simultaneously improve T3 triiodothyronine, T4 thyroxine and blood sugar GLU value and reduce oxidation index MDA value, does not cause adverse effects on other milk component indexes, can significantly improve the physical indexes of dairy cows, and has a good application prospect. The schizochytrium composition is added into daily ration of ruminants in the form of a feed additive by utilizing the transportation and enrichment capabilities of the schizochytrium composition and the DHA of the ruminants, and the liquid native DHA milk with good stability and high DHA content is finally secreted through rumen digestion, small intestine absorption, blood circulation transfer and mammary gland enrichment.

The invention discloses heat-resistant phaffia rhodozyma and a method for producing astaxanthin from straw hydrolysate by using the heat-resistant phaffia rhodozyma. The strain is classified and named as phaffia rhodozyma GF6, and the preservation number is CCTCC (China Center for Type Culture Collection) NO: M 2025649; the strain is inoculated into a fermentation culture medium, straw hydrolysate is used as a carbon source, and astaxanthin is efficiently produced at 28-30 DEG C; according to the method, the agricultural waste straw hydrolysate is used as a cheap crude raw material to replace all carbon sources to produce astaxanthin, so that the fermentation temperature is increased, the production cost is reduced, and the method is environment-friendly.

The invention discloses a preparation method of compound protease-yeast synergistic aroma enhancement seafood flavor powder, which comprises the following steps: firstly, mixing raw materials such as euphausia superba powder, dried oyster powder, skipjack powder, shrimp shell powder, shiitake mushroom powder, kelp powder, fish collagen peptide, glycine, L-alanine and glucose; the papain and bromelain with specific enzyme activity are subjected to composite enzymatic hydrolysis to create a flavor precursor. Then, the enzymatic hydrolysate is fermented through pichia pastoris, disodium 5 '-ribonucleotide (I + G) is added synchronously, and rich aroma components such as pyrazine and sulfide are generated; after fermentation, laver powder, cod-liver oil, a phaffia rhodozyma fermentation extract rich in astaxanthin and ginger oleoresin are added for flavor locking and oil immersion homogeneous emulsification, and finally, a microporous granular product is prepared through compound wall material embedding and spray drying. According to the preparation method, biological enzymolysis, microbial fermentation and flavor targeted locking technologies are integrated, so that the aroma level, strength, stability and cold water instant solubility of the flavor powder are improved.

The present application relates to the field of microbial technology, and particularly to a method for producing astaxanthin emulsion by Phaffia rhodozyma, which comprises the following steps: super-micro grinding dry Phaffia rhodozyma to extract astaxanthin with a solvent, dissolving the astaxanthin in soybean oil, using whey protein as the water phase, preparing O / W emulsion, adding tannic acid to protect astaxanthin from enzymatic cross-linking, and forming a "multi-layer" interface film through electrostatic assembly and enzymatic cross-linking. The "multi-layer" interface film of chitosan-sodium alginate improves the mechanical strength of the interface film and limits the penetration of oxidants into the internal oil phase to contact astaxanthin. The charge neutralization of sodium alginate grafted with polyethylene glycol prevents the flocculation of astaxanthin emulsion, and the enzymatic cross-linking of chitosan grafted with peptide segments realizes the targeted release of astaxanthin in the intestinal tract. The dosage of tyrosinase and the enzymatic reaction time are controlled to prevent the residual of tyrosinase with oxidizing property, and tannic acid prevents the oxidation of astaxanthin by oxidizing substances generated in the tyrosinase reaction.