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Medium temperature type astaxanthin producing bacterial strain and its culture process

A cultivation method and technology of astaxanthin, applied in the direction of fungi, fermentation, etc., can solve the problems of low astaxanthin cell content, complex production process, low cultivation temperature, etc., achieve short cultivation period, simple nutritional requirements, and cell growth quick effect

Inactive Publication Date: 2007-06-27
领先生物农业股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the low content of astaxanthin cells in the original strain of Phaffia yeast and the low temperature required for cultivation, the production process is complicated. At present, only a few large foreign companies can produce
The synthesis of astaxanthin by Phaffia yeast generally requires low-temperature cultivation (20-22°C), and the fermentation and cultivation process must be cooled down, which consumes more energy

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Mutation screening of mesophilic astaxanthin-producing bacteria

[0031] 1. Chemical mutagenesis

[0032] (1) Take the fresh bacterium activated for 30 hours (the starting strain is Phaffia rhodozyma JMC9042, purchased from Japan Institute of Physical and Chemical Engineering), centrifuge, discard the supernatant, suspend with 0.5ml of normal saline, add 0.1ml of NTG saturated saline solution, the effect 10 minutes;

[0033] (2) The reaction solution was centrifuged and washed three times with normal saline;

[0034] (3) The detoxified bacteria are suspended with 1ml of normal saline, and diluted to an appropriate concentration (the dilution is 10 6 -10 8 ) and spread on the PDA plate (part of the PDA medium contains 4-8 mg / L of screening agent simvastatin at an appropriate concentration), pH=6.0. Protect the plate from light, incubate upside down at 24°C for 7-10 days;

[0035](4) After the mutagenized cells were cultured upside down on the plate for 7-1...

Embodiment 2

[0040] Example 2 Cultivation of mesophilic astaxanthin-producing bacteria and determination of astaxanthin content

[0041] 1. Cultivation of mesophilic astaxanthin-producing bacteria:

[0042] (1) Seed culture in fermenter: Pick a single colony of the astaxanthin-producing bacteria, insert it into 50mL liquid seed medium, and cultivate it at 23°C and 200r / min for 36h. 600 =7.5; then according to the 5% inoculum size, the above-mentioned seed culture solution was inserted into 50 mL of the liquid seed medium, and cultivated at 23 ° C for 36 h at 200 r / min, and the culture solution OD 600 =7.5; then transfer the obtained seed culture solution into 100mL of the liquid seed medium according to the inoculum size of 5%, and cultivate at 23°C for 36h at 200r / min, the culture solution OD 600 =7.5;

[0043] (2) Feed-feeding fermentation: according to 5% inoculum size, the seed liquid that described step (1) obtains is inserted in the 7.5L fermentor that 4.0L liquid batch basal mediu...

Embodiment 3

[0050] Embodiment 3 Cultivation of mesophilic astaxanthin-producing bacteria and determination of astaxanthin content

[0051] 1. Cultivation of mesophilic astaxanthin-producing bacteria:

[0052] (1) Seed culture in fermenter: Pick a single colony of the astaxanthin-producing bacteria, insert it into 50mL liquid seed medium, and cultivate it at 24°C and 220r / min for 40h, and the culture medium is OD 600 =8.0; then insert the above seed culture solution into 50mL of the liquid seed medium according to the 5% inoculum size, cultivate at 24°C and 220r / min for 36h, and the culture solution OD 600 =8.0; then transfer the obtained seed culture solution into 100mL of the liquid seed medium according to the inoculum size of 5%, and cultivate at 24°C for 36h at 220r / min, the culture solution OD 600 = 8.0;

[0053] (2) Feed-feeding fermentation: according to 5% inoculum size, the seed liquid that described step (1) obtains is inserted in the 7.5L fermentor that 4.0L liquid batch basa...

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Abstract

The present invention provides Phaffia rhodozyma A-1 CGMCC No.1837 as one kind of medium temperature type astaxanthin producing bacterium. The present invention provides also the culture process of the medium temperature type astaxanthin producing bacterium. The medium temperature type astaxanthin producing bacterium has simple nutrient requirement for production and industrial production power consumption lower than that for low temperature type astaxanthin producing bacterium. The culture process of the medium temperature type astaxanthin producing bacterium is simple, short in culture period, high in astaxanthin yield and favorable to industrial production.

Description

technical field [0001] The invention relates to the field of astaxanthin production, in particular to a mesophilic astaxanthin-producing bacterium and a culture method thereof. Background technique [0002] Phaffia rhodozyma was isolated in early 1967 and named Phaffia rhodozyma in 1976. In 1995, some strains were found to have a sexual cycle and relocated to the Basidiomycota (Xanthophyllomycesdendrorhous). Its biological characteristics mainly include: withered red colony, repeated budding at a single cell site, cell wall containing xylose, diazo B staining positive, unique 18S rRNA sequence, producing astaxanthin in 3R, 3′R′ configuration. [0003] Astaxanthin is a carotenoid with the chemical name 3,3'-dihydroxy-B,B-carotene-4,4'dione. Astaxanthin is a fat-soluble pigment, insoluble in water, but easily soluble in organic solvents such as tetrahydrofuran, methylene chloride, chloroform, methanol, and ethanol. Natural astaxanthin has a special molecular structure. In v...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C12P23/00
Inventor 李颖王永星孟永宏徐志文
Owner 领先生物农业股份有限公司
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